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Evaluating causes of error in landmark-based data collection using scanners

  • Brian M. Shearer,

    Roles Conceptualization, Data curation, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

    Affiliations Ph.D. Program in Anthropology, The Graduate Center (CUNY), New York, New York, United States of America, New York Consortium in Evolutionary Primatology, New York, New York, United States of America, NYCEP Morphometrics Group, New York, New York, United States of America

  • Siobhán B. Cooke,

    Roles Data curation, Investigation, Visualization, Writing – original draft, Writing – review & editing

    Affiliations NYCEP Morphometrics Group, New York, New York, United States of America, Center for Functional Anatomy and Evolution, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

  • Lauren B. Halenar,

    Roles Data curation, Investigation, Writing – review & editing

    Affiliations New York Consortium in Evolutionary Primatology, New York, New York, United States of America, NYCEP Morphometrics Group, New York, New York, United States of America, Department of Biology, Farmingdale State College (SUNY), Farmingdale, New York, United States of America

  • Samantha L. Reber,

    Roles Data curation, Formal analysis, Investigation

    Affiliation School of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom

  • Jeannette E. Plummer,

    Roles Formal analysis, Investigation

    Affiliation Department of Archaeology, University of Sheffield, South Yorkshire, United Kingdom

  • Eric Delson,

    Roles Conceptualization, Funding acquisition, Writing – review & editing

    Affiliations Ph.D. Program in Anthropology, The Graduate Center (CUNY), New York, New York, United States of America, New York Consortium in Evolutionary Primatology, New York, New York, United States of America, NYCEP Morphometrics Group, New York, New York, United States of America, Division of Vertebrate Paleontology, American Museum of Natural History, New York, New York, United States of America, Department of Anthropology, Lehman College (CUNY), Bronx, New York, United States of America

  • Melissa Tallman

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

    tallmame@gvsu.edu

    Affiliations NYCEP Morphometrics Group, New York, New York, United States of America, Department of Biomedical Sciences, Grand Valley State University, Grand Valley, Michigan, United States of America

Evaluating causes of error in landmark-based data collection using scanners

  • Brian M. Shearer, 
  • Siobhán B. Cooke, 
  • Lauren B. Halenar, 
  • Samantha L. Reber, 
  • Jeannette E. Plummer, 
  • Eric Delson, 
  • Melissa Tallman
PLOS
x

Abstract

In this study, we assess the precision, accuracy, and repeatability of craniodental landmarks (Types I, II, and III, plus curves of semilandmarks) on a single macaque cranium digitally reconstructed with three different surface scanners and a microCT scanner. Nine researchers with varying degrees of osteological and geometric morphometric knowledge landmarked ten iterations of each scan (40 total) to test the effects of scan quality, researcher experience, and landmark type on levels of intra- and interobserver error. Two researchers additionally landmarked ten specimens from seven different macaque species using the same landmark protocol to test the effects of the previously listed variables relative to species-level morphological differences (i.e., observer variance versus real biological variance). Error rates within and among researchers by scan type were calculated to determine whether or not data collected by different individuals or on different digitally rendered crania are consistent enough to be used in a single dataset. Results indicate that scan type does not impact rate of intra- or interobserver error. Interobserver error is far greater than intraobserver error among all individuals, and is similar in variance to that found among different macaque species. Additionally, experience with osteology and morphometrics both positively contribute to precision in multiple landmarking sessions, even where less experienced researchers have been trained in point acquisition. Individual training increases precision (although not necessarily accuracy), and is highly recommended in any situation where multiple researchers will be collecting data for a single project.

Introduction

Over the last decade, landmark based three-dimensional geometric morphometrics (3DGM) utilizing digital specimen scans has become an increasingly integral tool in the fields of physical anthropology and paleontology. 3DGM allows researchers to analyze complex (i.e., non-linear) shape data through the application of landmarks to anatomically homologous points on multiple specimens [1]. Landmarks can be acquired either directly from a physical specimen, as with a Microscribe digitizer, or digitally via a computer program, such as Landmark Editor [2], on a virtual rendition of a bone. The latter method has become popular recently with the decreased price and increased ease-of-use of surface scanners, which allow researchers to create a permanent digital copy of a specimen for later use in landmark-based analyses and/or for storage and sharing with other researchers via an online database (e.g., www.morphosource.org). Many researchers have also begun using computed tomography scanners (CT) to digitally render their specimens when interested in both internal and external morphology, as dramatic increases in processing power of commercial computers and greater access to CT scanners has made this technology more practical in non-medical research (see [3,4,5] for reviews). Digital renderings of bony tissue from both surface and CT scanners are often treated as equivalent by researchers (e.g., [6]) and are used interchangeably based upon availability. However, there is no broadly consistent protocol for rendering digital scans or for applying landmarks to digital models, and the possibility that landmark-based 3DGM studies can potentially suffer from problems of inter- and intraobserver error as a result of these variables has not been thoroughly investigated (but see [7]).

In any landmark-based study using digitally rendered specimens there are multiple factors which may introduce error. Technological sources of error potentially include scanner type and brand (which inherently vary in their surface capture abilities based on design features) resolution at which a specimen is scanned, and the fitting and smoothing algorithms that may be used in post-processing of the surfaces that may differ per proprietary software programming idiosyncrasies. Scanning protocol-based sources of error result from the individual choices made by a researcher regardless of what scan technology they choose to utilize, and may include scanning methods (e.g., particular number of frames, scanning angle, or overall number of image families used at the discretion of the researcher), or reconstruction/rendering methods used that may include differences in a particular scan model refinement method (e.g., to what extent the “Mesh Doctor” function in Geomagic Studio or Wrap is used rather than a targeted refinement protocol using other available tools). User-based sources of error include differences in data collection experience among researchers, inherent researcher tendencies for precision and accuracy, and comprehension of instructions. Data collection-based sources of error involve repeatability of landmark protocols.

Landmarks are traditionally classified into three different types based on potential for anatomical homology. Type I landmarks are generally the most desirable type of landmark because of their ease of reproducibility and in identification of anatomical homology. They can be defined as points where multiple tissues intersect [8], for example, where the coronal and sagittal sutures meet (Bregm(A). Type II landmarks can be defined as points of potential homology that are based only on geometric evidence. Type II landmarks are often placed on the maxima or minima of structures, such as the tip of the canine. Type III landmarks are mathematically deficient in at least one coordinate, and are generally defined only with respect to other landmarks in that they characterize more than a single region of an object’s form [8]. Landmark types II and III are less desirable than Type I, as they are more difficult to accurately find and precisely mark, and generally describe structures that are not necessarily homologous in the traditional sense of the word [8], but are more likely to be mathematically or geometrically homologous. More recent research has introduced semilandmarks from 2D morphometrics [9,10] to 3DGM studies (e.g., [11]). Semilandmarks are used to compare the shapes of biological curves that are suspected to hold some functional or phylogenetic information but present an even more difficult case of repeatability. These curves are usually anchored with anatomically homologous landmarks which are also spaced equidistantly between the anchoring points. These points are then “slid” into their most “homologous” positions prior to multivariate analyses by minimizing either the bending energy or Procrustes distances in the sample (see [12] for an example of how both of these methods affect data processing). Semilandmark curves have been demonstrated to be most useful when applied over large surfaces that do not contain numerous traditional landmarks (e.g., the occipital bone of the cranium [13] or the trochlear surface of the tibia [14]).

Several researchers have conducted small-scale error studies examining between-scanner error and interobserver error with non-GM data and their results mostly suggest these types of error are of minimal concern. For example, Tocheri et al. [15] conducted an error study using non-landmark-based methods, in which they examined the variance in surface shape metrics of gorilla tarsals as collected by two researchers on virtual 3D models generated from both CT and laser surface scanners. They found that laser scan surfaces and those extracted from CT scans were not distinguishable, and that the two individuals who rendered and collected the data did not do so in a statistically different fashion. Likewise, Sholts et al. [16] measured scan model area and volume when constructed with multiple protocols and by two different individuals. They report intra- and interobserver error in scan construction at 0.2% and 2% variance, respectively, which they interpret as non-significant for scan sharing.

In a study conceived concurrently with this one, Robinson and Terhune [17] compared both inter- and intraobserver error rates between the two researchers on 14 differently sized crania of 11 primate taxa using traditional linear measurements, tactile 3D landmarking (i.e., Microscribe), and digital landmarking of computer rendered models. In regards to variance levels when applying landmarks to digital 3D models for morphometric analyses, they demonstrate negligible differences in rates of error between how scans were created (e.g., NextEngine vs CT), and that interobserver variation is higher than both intraobserver and intraspecific variation. Conversely, Fruciano and colleagues [18] also compared intra- and interobserver rates between two researchers using three different surface scan methodologies for a series of marsupial crania. These researchers found significant differences in landmark protocols both between observers and among the different scan types, and found that the differences in landmark collection protocols led to statistically different results when estimating phylogenetic signal in their dataset.

These studies demonstrate that training and a consistently applied protocol could reduce some technological and user-based error, although many of these results are contradictory. All previous studies thus far fail to address the possibility that in-person training may be impractical or impossible in some cases, and they use only three scan types while a wide variety of scanners is currently available on the market. Additionally, with the involvement of many more researchers of varying expertise levels, this study will provide more robust results regarding the magnitude of potential interobserver error.

As landmark-based studies increasingly move toward the use of surface scanners for creating virtual specimens of fossil (e.g., [19,20, 21, 22]) and extant (e.g., [23, 24, 25]) organisms that can be archived for sharing and future use, questions addressing the compatibility of data collected by different researchers with inherently different methods and equipment are paramount if truly collaborative and accurate research is to be achieved. Quantifying and understanding how intra- and interobserver error are affected by both technology and user error is especially relevant now as data sharing efforts are becoming common in the paleoanthropology and paleontology communities through open-access web databases like PRIMO (http://primo.nycep.org) and MorphoSource (www.morphosource.org), where both morphometric data and raw scans are shared freely among researchers.

Given the multiple potential sources of error in any landmark-based study, our goal here is to investigate whether landmarks can be placed at truly homologous points given the inherent differences in researcher experience, landmarking techniques, and the quality of a digital model resulting from different scanners and scanning protocols. To evaluate the gravity of some of these issues, we assess the compatibility of landmark data gathered by nine researchers with varying degrees of experience on scans of a single macaque cranium digitally rendered by four different scanners (see Table 1). We apply multivariate statistics to evaluate rates of precision and accuracy among researchers, and test the following three predictions:

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Table 1. List of scanners and scanner types used for this project.

Faces refers to the number of triangles in a surface.

https://doi.org/10.1371/journal.pone.0187452.t001

  1. Higher scan quality (as determined by higher resolution and point density) will reduce both intra- and interobserver error.
    We here aim to test if the differences in surface rendering inherent to different scanners will influence the ability of a researcher to both precisely and accurately landmark a digital scan model. We predict that higher scan quality will enable researchers to more accurately and precisely landmark digital specimens, regardless of training or levels of experience.
  2. Increased experience with 3DGM and/or osteology will decrease both intra- and interobserver error.
    We here assess whether experience positively correlates with both accuracy and precision in the ability of a researcher to apply landmarks to a 3D model. We predict that users with more osteological and morphometric experience will have lower rates of intraobserver error, and also that rates of interobserver error will be significantly less among these experienced individuals. We expect researchers with low levels of experience to have high rates of both inter- and intraobserver error. We predict a positive correlation with experience and precision/accuracy.
  3. In-person training provided by a single, experienced researcher will decrease both intra- and interobserver error rates of researchers that receive it.
    We here test whether personal instruction on how to collect landmarks has any influence on rates of variance. We predict that training will cause a reduction in interobserver error among those individuals that received it, and that it will significantly reduce intraobserver error for those trained individuals as compared to those without in-person training.

Finally, we also evaluate the efficacy of sliding semilandmarks for inter- and intraobserver error reduction.

Materials and methods

Materials

Digital models of an adult male Tibetan macaque (Macaca thibetan(A) cranium (American Museum of Natural History [AMNH] Mammalogy Department 129) were generated with two laser surface scanners (NextEngine Desktop 3D Scanner HD and Minolta Vivid 910), a structured white light scanner (the Breuckmann OptoTOP-HE), and a computed tomography (CT) scanner (General Electric Phoenix v|tom|x s240) (See Table 1; Figs 1 and 2). Laser surface scans were digitally processed in 2011 using Geomagic Studio 12 (now 3D Systems), white light scans were processed in OPTOCAT (the native Breuckmann editing software package), and CT scans were processed using VGStudio Max (Volume Graphics). For surface scans, post-processing was limited to the removal of extraneous material digitized by the scanner (e.g., the turntable on which the specimen was placed, any modeling clay used for support, etc.), curve-based hole filling, and refinement of minor mesh artifacts unavoidably generated during the scanning process (e.g., small spikes and poorly fitted surfaces).

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Fig 1. Scan comparison anterior view of Macaca thibetana (AMNH 129).

(A) Breuckmann OptoTOP-HE; (B) GE Phoenix v|tome|x s240 CT scan; (C) Minolta Vivid 910; (D) NextEngine 3D Scanner HD.

https://doi.org/10.1371/journal.pone.0187452.g001

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Fig 2. Scan comparison inferior view of Macaca thibetana (AMNH 129).

(A). Breuckmann OptoTOP-HE; (B) GE phoenix v|tome|x s240 CT scan; (C) Minolta Vivid 910; (D) NextEngine 3D Scanner HD.

https://doi.org/10.1371/journal.pone.0187452.g002

Methods

Scans were imported into the program Landmark Editor [2] where nine researchers (hereafter referred to as R1, R2, R3, etc.) with varying degrees of expertise as denoted by the suffixes (LX) for low experience, (MX) for medium experience, (HX) for high experience, and (T) for trainer (Table 2) placed thirty-seven Type I, II, and III landmarks and three three-dimensional semilandmark curves (Fig 3). The experience designation is based on the overall osteological knowledge and prior exposure to 3D geometric morphometrics methods. Each semilandmark curve was defined using three Type I, II or III landmarks as “anchors”; a series of 10 semilandmarks were automatically generated equidistant from one another along that curve (see Fig 3 and Table 3). The application of semilandmark curves was independent of other landmarks, even though they may share a point as an “anchor”, as Landmark Editor allows for the joining of multiple curves. This dataset was designed to reflect commonly used osteometric points and to cover often-studied areas of the cranium. All researchers who landmarked crania were given a written description of the landmark points (see Table 3), and an illustration of the points as defined by R9. For the researchers trained in person by R9, a pre-landmarked “atlas” cranium was included each project file to serve as a reference for those with less osteological experience and R9 was available to answer any questions and give clarifications. No additional assistance was given beyond these tools during the landmarking trials.

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Fig 3. Landmarks employed in this study.

Digital rendering of an adult male Macaca thibetana cranium (AMNH Mammalogy 129) with points depicting the 37 single landmarks (white dots) and three curves (black dotted lines) used in this study.

https://doi.org/10.1371/journal.pone.0187452.g003

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Table 2. List of observers who collected data, their experience, and the order in which they landmarked the scan replicates (scanner abbreviations from Table 1).

Each observer is designated by both a number (e.g., R1, R2, R3) and an experience abbreviation: LX = low experience, MX = medium experience, HX = High experience, T = Trainer. Experience designations were assigned based on overall osteological knowledge and familiarity with 3D GM methods and practice.

https://doi.org/10.1371/journal.pone.0187452.t002

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Table 3. List of landmarks used in this study.

Bilateral landmarks denoted by (L) and (R) for their respective anatomical sides. Quotation marks indicate identical description to point listed directly above. SLC = Semilandmark curve. For inclusion in sets, F = Full landmark set, R = Reduced landmark set, and S = Semilandmark only set. This landmark definition set and an illustrated atlas were provided to each researcher before their respective landmarking trials.

https://doi.org/10.1371/journal.pone.0187452.t003

Three landmark configurations were analysed to test the relative stability and usefulness of various landmark types:

  1. a “Full” landmark set consisting of all points initially described in the landmark protocol, including Type I, II, and III landmarks, and additionally a series of semilandmark curves.
  2. a “Reduced” landmark set including most Type I, II and III landmarks, but with semilandmarks and the most variable Type II and III landmarks removed (Landmarks 25, 26, 29, 30, 32 and 33). This landmark set was evaluated to test the variance on only relatively ‘stable’ and easily found landmarks, thereby potentially limiting the influence of difficult to find (or easily damage(D) points on dry crania.
  3. a “Semilandmark only” set consisting of only those points joined together by the curve function of Landmark Editor (points 38 through 67). These semilandmarks were applied independently from other landmarks during the initial “Full” landmark set application.

The Reduced landmark set and Semilandmark only set were created post hoc by removing points from the Full landmark set according to the specifics of each protocol as listed above, which were then independently tested to verify the influence of different point configurations. All statistical tests were performed on each of the three landmark sets in independent iterations. Additionally, the amount of variance was calculated for each individual landmark point to assess which discrete landmarks (or landmark types) are most prone to user error.

Each researcher placed the full landmark set on 10 replicates of the macaque cranium from each scanner (i.e., 10 replicates of the Breuckmann OptoTOP-HE scan, 10 replicates of the NextEngine scan, etc.) to assess variation in user accuracy and precision. Each user placed their landmarks on the different scans types in unique orders so as not to bias the results due to practice (see Table 2). The Reduced and Semilandmark only sets were subsequently analyzed by removing points prior to all relevant geometric morphometric analyses (See Table 3). Semilandmark sliding is a technique used with semilandmarks to “slide” them into their most homologous positions by either minimizing the bending energy or Procrustes distance among specimens [9, 26]. The purpose of these analyses was to assess sources of error, and all data were collected on the same cranium; therefore, sliding semilandmark protocols were not employed here as there are no issues with homology between specimens.

Landmark coordinates were exported to morphologika v2.5 [27] which was used to perform a generalized Procrustes analysis (GPA). This analysis translates, scales, and rigidly rotates specimen configurations around a common centroid, using a least-squares algorithm to optimally minimize the distance each shape lies from the origin [28,29,30]. A separate GPA was performed for each observer to assess inter-scan error and intraobserver error. A GPA of the entire pooled dataset was used to assess interobserver error.

In addition to landmarking replicates of the same cranium, Researchers 6 (HX) and 8 (HX) placed the full landmark configuration on a total of 10 female macaque crania from 7 different species to compare the magnitude of interobserver error to normal species and inter-species shape differences (see Table 4). Steps of this second data collection were identical to those previously listed for the adult female M. thibetana cranium (AMNH Mammalogy 129). In this instance, all analyses were performed both with and without sliding the semilandmarks as there were different crania as part of the dataset. For this analysis including specimens of multiple taxa, semilandmarks were slid into their most homologous positions by minimizing the Procrustes distances among the specimens. All analyses were completed in the geomorph package for R [31].

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Table 4. Sample of Macaca used for testing the magnitude of interobserver error.

https://doi.org/10.1371/journal.pone.0187452.t004

Effects of landmark position on error.

The variance for each individual landmark was assessed by computing the average Procrustes distance between the mean landmark position and each individual replicate for each researcher. In this instance, the data collected by each researcher were subject to a separate GPA. The variance for each landmark was also calculated for the entire dataset. In this case, all data from all users were subjected to a single GPA and the same process was followed for computing the mean error for each landmark.

Effects of scan type on error.

The amount of intraobserver error per scan type was calculated for each individual for each landmark configuration. Intraobserver error was calculated as the Procrustes distance (defined as the square root of the sum of squares distances between corresponding landmarks of shapes after superimposition [9]) between each replicate and the mean for all replicates for each scan from a single researcher. Significant differences in error among scan types were assessed using an ANOVA with Tukey’s pairwise post hoc comparisons to determine whether intraobserver error was significantly lower for any particular scanner. Box plots were generated in PAST v 3.0 [32] to illustrate differences in variance among scan types for each researcher; solid lines indicate median variance, the boxes indicate the 25–75% quartile, and the whiskers extend to the farthest data point that is less than 1.5x the height of the box. Finally, all Procrustes distances from the mean from all nine researchers for each scan type were pooled. A boxplot illustrating the distribution of distances for each scan type was produced in PAST [32]. An ANOVA with Tukey’s post hoc comparison was performed to determine if there was an overall mean difference in rates of intraobserver error among the scan types. A two-way ANOVA with Tukey’s post hoc pairwise comparisons was performed to determine whether there were significant differences between scan types when differences among researchers were also part of the model.

The amount of interobserver error for each scan type was recorded as the series of pairwise Procrustes distances between all different users for each scanner. Boxplots were created using PAST [32] to illustrate the range of pairwise Procrustes distances. Significant differences among the ranges of pairwise Procrustes distances were tested using an ANOVA with Tukey’s post hoc pairwise comparisons.

Effects of experience on error.

To compare the degree of intraobserver error among researchers, we examined the total intraobserver error for each individual using the range of Procrustes distances from the mean using all forty replicates. Box plots of these data were generated in PAST [32] to illustrate differences in intraobserver error among users as described previously. An ANOVA with Tukey’s post hoc pairwise comparisons was performed to determine if there were significant differences among users in the degree of intraobserver error.

In order to explore whether experience influenced patterns of intraobserver error, principal components analyses (PC(A) were generated with MorphoJ [33]. Percent variance on the first three axes was also recorded. If the percent variance accounted for by each axis is low, variation in landmark placement is occurring isotropically as variance is occurring in many different directions. If percent variance is high on the first axis, it indicates that error is occurring anisotropically for certain landmarks.

Effects of training on error.

A PCA of the Procrustes aligned coordinates for all trials for all users was performed and the first two principal components were visualized. If in-person training had a positive effect on landmark consistency, those individuals who received training should appear in a common area of the morphospace. In addition, a UPGMA dendrogram constructed using average Procrustes distances among researchers was also created using PAST [32] to see if users receiving in-person training formed a single cluster.

Interobserver error vs. shape variability in multiple species.

Interobserver error was calculated as the Procrustes distance between each replicate and the mean of the entire dataset. To assess whether rates of interobserver error (with and without training) were larger than a real biological signal, the pooled interobserver error rates for all researchers and trials on the single M. thibetana cranium were plotted in three boxplots with the pooled error rates for the seven different macaque species landmarked by R6 (HX) and R8 (HX).

Results

Effects of landmark type on error

The results for intra- and interobserver error at each landmark are presented in Table 5. In terms of intraobserver error, there was no discernable pattern for which landmarks were always the most or least error prone. However, Landmarks 25, 26, 29 and 30 commonly had relatively high levels of intraobserver error. Landmark 3 had one of the lowest intraobserver errors in seven out of nine researchers, and landmarks 14, 21 and 35 also commonly had relatively low levels of intraobserver error. There were six landmarks that had much higher interobserver errors when compared to all of the other landmarks. Those landmarks were 25, 26, 29, 30, 32 and 33 and were removed from the Reduced landmark configuration in all subsequent analyses. These are all Type III landmarks and as such were expected to be the most error prone.

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Table 5. Average Procrustes distance from the centroid to each replicate for every Type I, II or III landmark in the analysis.

Data for individual Procrustes alignment indicate that only the 40 replicates for each individual were used in the calculation; full Procrustes alignment includes all replicates for all individuals in a single Procrustes alignment. Bolded values indicate the six largest average Procrustes distances for the alignment using all users; these were the landmarks removed in the Reduced Landmark dataset.

https://doi.org/10.1371/journal.pone.0187452.t005

The effects of scan type on error

Table 6 tabulates the average Procrustes distances from the mean shape among replicates for each user and each scan type for all three landmark configurations. These results can also be visualized as box plots in Fig 4. The results from one way ANOVAs indicate that there were some significant differences in variance among the scan types for a single researcher; however, post hoc pairwise comparisons revealed no consistent pattern explaining which pairs of scan types were significantly different from one another. Some users exhibited a trend toward similar levels of variance for scans which were landmarked in sequential order (R1 (LX), R3 (LX), and R4 (MX)), while others (R2 (MX), R3 (LX), R6 (HX), R7 (MX), and R8 (HX)) exhibited no discernible pattern in their landmarking variability. When all trials from all researchers were pooled, results of ANOVAs showed that there were no significant differences present among scanning types (p = 0.12 for the Full configuration, p = 0.88 for the Reduced configuration and p = 0.13 for the Semilandmark only configuration; Fig 5 and Tables 79). Thus, average intraobserver error was statistically uniform across scan types and for all three landmark configurations when users are considered as one group.

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Table 6. Average variance for intraobserver trials for different scan types for the entire landmark protocol.

https://doi.org/10.1371/journal.pone.0187452.t006

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Fig 4. Box plot illustrating the amount of intraobserver error for each user with each scanner using each landmark set.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set. See Table 6 for numerical data.

https://doi.org/10.1371/journal.pone.0187452.g004

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Fig 5. Box plot illustrating the amount of intraobserver error for each scanner type for each landmark set.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set.

https://doi.org/10.1371/journal.pone.0187452.g005

When both user and scanner are taken into account, two-way ANOVAs show that there is a significant difference in levels of intraobserver error between the NextEngine and both the CT and Minolta scanners for the Full and Semilandmark data sets (Tables 1018). However, the effect size (as measured by the mean difference in intraobserver error between scanners) is smaller than the average intraobserver error for any user (Table 6). There is no significant difference among scanners for the Reduced landmark dataset.

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Table 10. Results of a two-way ANOVA for user and scanner for the Full landmark set.

https://doi.org/10.1371/journal.pone.0187452.t010

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Table 11. Tukey’s post hoc pairwise comparisons for scanners for the Full landmark set.

https://doi.org/10.1371/journal.pone.0187452.t011

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Table 12. Tukey’s post hoc pairwise comparisons for users for the Full landmark set.

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Table 14. Tukey’s post hoc pairwise comparisons for scanners for the Reduced landmark set.

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Table 15. Tukey’s post hoc pairwise comparisons for users for the Reduced landmark set.

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Table 17. Tukey’s post hoc pairwise comparisons of scanning types for the Semilandmark dataset.

https://doi.org/10.1371/journal.pone.0187452.t017

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Table 18. Tukey’s post hoc pairwise comparisons of users for the Semilandmark dataset.

https://doi.org/10.1371/journal.pone.0187452.t018

Fig 6 illustrates the distribution of pairwise Procrustes distances among different users–the equivalent in this case to interobserver error—among scan types for each of the three configurations. ANOVAs show no significant differences in the distribution of interobserver error among the four scanners tested for any of the three landmark configurations.

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Fig 6. Boxplot of the distribution of pairwise Procrustes distances between different users for each scanner and landmark configuration.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set.

https://doi.org/10.1371/journal.pone.0187452.g006

Effects of user experience on error

Fig 7 and Table 6 illustrate the variance in pairwise Procrustes distances for each researcher by landmark configuration. In most cases, researcher experience strongly correlated with levels of variance; less experienced researchers had higher levels of variance (e.g., R2 (MX) and R3 (LX); Table 18) and more experienced researchers had lower levels (e.g., R5 (HX), R6 (HX) and R9 (T)). Interestingly, Researcher 4 also had low levels of variance overall despite having equivalent experience as R2 (MX) and R7 (MX), so factors other than experience can play a role in obtaining a higher level of precision. R1 (LX) had the least experience and had relatively high levels of variance except in semilandmark placement where the researcher had lower variance than the others. R8 (HX) has intermediate levels of variance, sometimes being quite low and other times being quite high. For instance, R8 (HX) had lower levels of variance for the Reduced landmark set, except for the NextEngine trials, but much higher levels of variance for the curve set, regardless of scan type (Fig 7).

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Fig 7. Boxplot illustrating the range of intraobserver error for each researcher for all forty trials.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set.

https://doi.org/10.1371/journal.pone.0187452.g007

To examine rates of intraobserver error, we used ANOVA analyses with Tukey’s post hoc pairwise comparisons. For the Full landmark configuration, R4 (MX) and R6 (HX) were not significantly different from each other in landmark placement, but both had significantly lower rates of intraobserver error than other researchers. R3 (LX) and R7 (MX) were also not significantly different from each other, but both had significantly higher rates of intraobserver error. In the Reduced landmark set, there were no significant differences between R4 (MX), R5 (HX), R6 (HX) and R9 (T), but all four had significantly lower intraobserver error rates than the rest of the researchers. For the Semilandmark set, R3 (LX) had significantly higher values than all other researchers. R1 (LX), R3 (LX) and R6 (HX) were all not significantly different from each other, and all had significantly lower intraobserver rates than R5 (HX), R7 (MX), and R8 (HX) (in addition to R3 (LX)). The other researchers had mid-range values and did not form any cohesive groups.

Variability on the level of the individual can be seen in the results of the percent variance on the first three axes of our principal components analyses for all scans (Table 19). In most cases, the percent variance on the first three axes was relatively uniform; however, both R5 (HX) and R7 (MX) showed a higher proportion of variance on the first PC axis. Landmarks 1, 2, 13, 22, 23, 32 and 33 commonly had the greatest variance, and landmarks 3 and 31 the least; however, there was no consistent pattern as to the direction in which these landmarks varied for each user and no correlation between variance in location of these landmarks and scan type, suggesting these differences were stochastic in nature. In addition, no consistent pattern emerged when visualizing which landmarks contributed most to differences in landmark positions among scanners for each user along the first three principal axes.

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Table 19. Percent of variance on the first three axes from principal component analyses by user for each landmark set combining all scan types and replicates (n = 40 combined scans per user).

https://doi.org/10.1371/journal.pone.0187452.t019

Effects of in-person training on error

Fig 8 depicts a PCA plot of all the iterations of the full landmark set for all researchers. R2 (MX), R3 (LX), R4 (MX), and R7 (MX) all received individual training from R9 (T) and broadly overlap in their landmark placements towards the center of the PC axes for the full landmark set (Fig 8(A). R1 (LX) also received in-person training, but falls farther away from R9 (T) on PC 2. R6 (HX) has similar values to the training group on PC 2 but falls more towards the negative axis of PC 1. R8 (HX) is different from the training group on both PC 1 and PC 2. For the Reduced landmark set (Fig 8(B), there is almost complete overlap between R2 (MX), R3 (LX), R4 (MX) and R7 (MX), all of whom had in-person training. R1 (LX) partially overlaps with this group. Two of the trials from R9 (T) fall with this group, but most of R9 (T)’s trials are separated from the training group on both PC 1 and PC 2. R5 (HX) and R6 (HX) fall with the training group on PC 1 but not PC 2. Again, R8 (HX) is farther away on both axes. In the Semilandmark only set (Fig 8(C), PC 1 accounts for the differences among researchers while PC 2 represents variation related to intraobserver error. Most of the researchers with in-person training fall with R9 (T) on this axis. R6 (HX) and R8 (HX) are most distant from this cluster at the positive end of PC 1, while R5 (HX) with just in-person clarification of details falls on the negative end of this axis.

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Fig 8. PCA plots of all trials from all users.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set.

https://doi.org/10.1371/journal.pone.0187452.g008

Removing users who had no in-person training from R9 (T) did improve average interobserver error for two of the datasets. Average interobserver error was improved for the Full landmark (0.12 to 0.10) and Semilandmark only sets (0.14 to 0.11) but not for the Reduced landmark set (0.08) (Fig 9). A dendrogram (Fig 10) based on each landmark set of all trial iterations indicates that most users who received in-person training from R9 (T) clustered with R9 (T) for the Full and Semilandmark only datasets. In the Full dataset (Fig 10(A), two experienced users with no input from R9 (T) (i.e. R6 (HX), R8 (HX)) form an outgroup cluster to the remaining researchers that did receive training, excepting R5 (HX), who clusters as a sister group of R9 (T) plus trainees to the exclusion of R1 (LX) and R3 (LX), who also received in person training from R9 (T). For the Reduced landmark set, four of five users who received training (R2 (MX), R3 (LX), R4 (MX), and R7 (MX)) from R9 (T) form a cluster with each other, and R9 (T) forms a group with R1 (LX) (trainee) in a separate cluster. R5 (HX) and R8 (HX) (who received no in-person training) fall outside the trainee group, although R6 (HX) falls as sister to the main trainee cluster, suggesting some similarity in marking with the Reduced landmark set. Using the Semilandmark only set, the dendrogram clusters all trainees except for R1 (LX) close to the trainer R9 (T), although R5 (HX) (non-trainee) splits the two groups.

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Fig 9. Boxplots illustrating the change in interobserver error when those without in-person training were removed.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set.

https://doi.org/10.1371/journal.pone.0187452.g009

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Fig 10. UPGMA dendrograms illustrating how different researchers cluster.

(A) Full landmark set; (B) Reduced landmark set; (C) Semilandmark set. Gray areas represent individuals that received in-person training by R9.

https://doi.org/10.1371/journal.pone.0187452.g010

Interobserver error vs. shape variance among multiple specimens

Fig 11 illustrates a comparison between the range of inter- and intraobserver error for two researchers (R6 (HX) and R8 (HX)) compared to the range of shape difference among the crania of ten different macaques from seven different species. For the Full data set, average interobserver error was greater than the differences between different macaques. However, for both the Reduced and the Semilandmark only set, the average difference between different macaques was greater than interobserver error (Table 20). That said, in all three landmark configurations the range of pairwise Procrustes distances representing interobserver error overlapped substantially with the range of pairwise Procrustes distances between the different macaque crania. In addition, the distribution of pairwise Procrustes distances representing intraobserver error also overlapped with the distribution of pairwise Procrustes distances between different macaques for the Semilandmark only set for both researchers. Intraobserver error for R8 (HX) also slightly overlapped the differences among macaques for the Full and Reduced landmark configurations; intraobserver error for R6 (HX) did not overlap the distribution of pairwise Procrustes distances for different macaques at all for these two datasets (Fig 11).

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Fig 11. Boxplots comparing inter- and intraobserver for Researchers 6 and 8 relative to the variation found among difference species of Macaca.

These data are from the full landmark set.

https://doi.org/10.1371/journal.pone.0187452.g011

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Table 20. Average pairwise Procrustes distance between landmarked trials by the same user (intraobserver error), landmarked trials between two different users (interobserver error) and between different macaques.

https://doi.org/10.1371/journal.pone.0187452.t020

In both landmark sets, sliding semilandmarks reduced intraobserver error as well as the differences among the different macaques (Fig 12). Sliding the semilandmarks seemed to have the most obvious impact on intraobserver error vs. the differences among the macaque crania for each of the users separately. For instance, for R6 (HX), after semilandmark sliding there was almost no overlap between the range of Procrustes distances among the repetitions and among the different macaques for the Semilandmark only set. However, sliding the semilandmarks did not have an appreciable effect on lowering the interobserver error; in fact, for the Full configuration, mean interobserver error increased as compared to no semilandmark sliding (Table 20). In both landmark sets mean interobserver error is close to the mean Procrustes distance between different macaque crania.

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Fig 12. Boxplots comparing inter- and intraobserver error Researchers 6 and 8 to the variation in different species of Macaca for the Full and Semilandmark only configurations after semilandmark sliding.

(A) Full landmarkset; (B) Semilandmark set. Note the low amount of pooled intraobserver error relative to the large amount of interobserver error between the researchers and relative to the amount of variation in different species of macaques for both landmark configurations.

https://doi.org/10.1371/journal.pone.0187452.g012

Discussion

Here, we present results of an error study comparing compatibility of scan types–which vary by instruments and scan acquisition protocol–on user-gathered landmark data to determine the extent to which error within and among individuals can influence the outcome of a geometric morphometric study. We evaluated these factors to determine whether or not it is sound practice to combine data collected from multiple scanners and/or by multiple individuals. The trend of data sharing and increased availability of both scan and landmark data present challenging questions about both compatibility of datasets and repeatability of landmarks given the potential that a researcher may use multiple scanners for a project and involve multiple co-workers in data collection. Overall, we observed three major trends in our data and offer suggestions on how to mitigate the problems arising from such trends:

(1) Error rates appear to remain consistent among and within users regardless of overall scan quality or type

Based purely on visual assessment, distinctly different digital models result from all the surface scanners and CT scanner tested here (see Figs 2 and 3), each with clearly observable differences in surface texture and resolution. For example, the two laser surface scanners do not capture the morphology of the teeth well, most likely due to the refractive properties of enamel and/or lower inherent resolving power. Similarly, complex structures like the basicranium are not captured as well by the laser surface scanners when compared to the white light scanner and the CT scanner.

When all researchers are considered together, no distinct pattern emerges to designate a clearly superior scan type to reduce landmark error. There were significant differences among scan types at the level of an individual researcher, but there was no pattern as to which scan types were significantly different from one another, or which scan types resulted in the lowest levels of intraobserver error. In other words, any statistically significant differences in any researcher's trials do not reflect a broad pattern, but rather more likely reflect individual inconsistencies in landmarking. Thus, despite the visible differences, scan model was not found to significantly influence most researchers' abilities to place landmarks and did not affect overall intra- and interobserver error rates (see Table 18 and Figs 4 and 5). This finding is consistent with that of Terhune and Robinson [17] although not with Fruciano and colleagues [18]. That said, Fruciano and colleagues [18] used a different set of scan types than this study or Terhune and Robinson [17]. Additionally, Fruciano and colleauges [18] reduced the complexity of their higher resolution scan (taken by a Solutionix Rexcan CS+ scanner) to match the triangle count of the Nextengine scanner, which is a protocol that neither Terhune and Robinson [17] or we report as part of our model construction protocol. This difference in post-processing may account for some of the reported differences. Finally, we did find some significant differences among surface scanners in this study, though the effect size was similar to (or smaller than) intraobserver error. Similar metrics are not reported in Fruciano et al. [18], so it is difficult to determine whether their results match this study in term of effect size. However, differences in initial design are apparent, and have undoubtedly influenced the results of our separate studies. As Fruciano et al. [18] differed from our study in several ways (e.g., smaller number of participants, narrow range of participant experience, exclusive use of Type I landmarks), we expect that the discrepancies with our results are likely the downstream effects of differences in basic design features.

In this study, as higher scan quality did not consistently reduce error and lower scan quality did not increase error, we believe that scanner type may reflect a case of diminishing returns, whereby even the lowest quality modern scanner will maintain a resolution sufficient for accurate and precise landmarking, while higher resolution scanners may not improve on this model resolution drastically enough to influence results. On the other hand, such differences in resolution may impact the clarity of the scan when used in observations of morphology, e.g., for scoring characters to be used in a cladistic analysis, a question not addressed here.

(2) Users with more osteology and 3DGM experience generally had less intraobserver error, but experience with osteology or morphometrics did not improve interobserver error

Researchers with little experience were less likely to be consistent within their own scan iterations, but researchers with extensive levels of experience did not necessarily agree on point collection protocol, and therefore have similar levels of interobserver variance as the inexperienced users. For example, R1 (LX), R4 (MX), R6 (HX), and R9 (T) maintained high precision throughout their trials but disagreed on what constituted accurate landmark placement. The data clusters for R1 (LX) and R4 (MX) occupy a similar morphospace on PC 1, but are on opposite ends of PC 2, a trend that R6 (HX) and R8 (HX) also share, although both R6 (HX) and R8 (HX) are shifted to the positive end of PC 1 relative to R1 (LX) and R4 (MX).

However, if broken into two groups—those that received in-person training in point collection from R9 (T) and those that did not—individuals who received training in landmark placement had lower average interobserver error rates when compared with each other than those that did not for the landmark configurations including semilandmarks. This trend persists despite the fact that the group that received training had relatively greater intraobserver error and less overall experience. These results suggest that in-person training for a particular landmark collection protocol could be critical in mitigating the effects of interobserver error, but we acknowledge that this is an impractical step for researchers interested in sharing their landmark data via digital media. We therefore suggest planning ahead if intending to combine landmark data from multiple researchers by providing at the start of a project extremely detailed data collection guides where relevant with photographs and clear written descriptions, i.e., a higher level of training than was provided by R9 (T) in this study, especially for datasets that include semilandmarks. Additionally, a pre-landmarked “Atlas” specimen provided by the dataset’s originator may prove useful as a template exemplar for less experienced users or for complex point arrangements, although to what extent this may improve rates of interobserver error remains to be tested. We recommend that any study using landmark data from multiple researchers must be carefully designed with these potential sources of error in mind from the start; it is not advisable to simply mine online databases, or make requests of colleagues for previously collected landmark data to combine into one master data set. Detailed guides and initial supervision are critical for any study combining data from multiple sources.

(3) Interobserver error was consistently higher than all other potential error types observed among researchers in this study

Our results suggest that interobserver error is of much greater concern than intraobserver error for different scan types or scan iterations. The average amount of variance between users landmarking a single cranium was roughly equivalent to, and in some cases greater than, the average amount of shape variation found among single cranial representatives from ten different macaques (Fig 12). R6 (HX) and R8 (HX) were chosen among the HX researchers to complete this trial; it is possible that interobserver error would have been substantially lower had different researchers completed this set of trials. Sliding semilandmarks improved intraobserver error in these trials, but actually increased interobserver error, so we do not recommend using semilandmark sliding as a strategy to decrease interobserver error. This finding impels caution in combining scan-based 3DGM datasets without first conducting numerous error tests to minimize variance. The potential for noise to mask real biological differences is a genuine concern for many researchers, and combining data collected by multiple individuals may in fact overwhelm any real signal in data.

Conclusions

Overall, our results suggest that interobserver error is of much greater concern than intraobserver error for different scanners or scan iterations in 3DGM studies using landmarks collected on virtual specimens. The average amount of interobserver error on the same specimen was approximately equivalent to the average pairwise Procrustes differences among ten different macaques, suggesting that interobserver error may be mistaken for real biological differences where none actually exist if data collected by multiple users are combined in a study. As such, our results impel caution when attempting to combine landmark-based datasets from multiple individuals, and we suggest that multiple error studies be conducted within and among involved researchers to mitigate both intra- and interobserver error before data collection intended for publication is conducted. Our results also suggest that error rates can be reduced if researchers participating in a study receive specific, in-person instruction from one individual or agree via consensus on data collection protocols. Digital data sharing efforts in morphometrics should be approached with great caution unless the consistency of a landmarking protocol is carefully verified in this way. Moreover, as scanner type appears to have minimal influence on landmark variance, we encourage that scans, rather than landmarks, should be shared.

Acknowledgments

We thank the Department of Mammalogy of the American Museum of Natural History and senior scientific assistant Eileen Westwig for access to specimens. We thank the Division of Paleontology, American Museum of Natural History for the space allocated to NYCEP to perform this study. We also thank the AMNH Microscopy and Imaging Facility managers (especially Morgan Hill and James Thostenson) for their assistance with CT imaging. Finally, this study could not have been completed without assistance from some of our volunteer researchers: Diandra Kay Atkinson, Jacquelyn Spiegel and Brian Schilder.

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