SHARPIN (Shank-Associated RH Domain-Interacting Protein) is a component of the linear ubiquitin chain assembly complex (LUBAC), which enhances TNF-induced NF-κB activity. SHARPIN-deficient (Sharpincpdm/cpdm) mice display multi-organ inflammation and chronic proliferative dermatitis (cpdm) due to TNF-induced keratinocyte apoptosis. In cells, SHARPIN also inhibits integrins independently of LUBAC, but it has remained enigmatic whether elevated integrin activity levels in the dermis of Sharpincpdm/cpdm mice is due to increased integrin activity or is secondary to inflammation. In addition, the functional contribution of increased integrin activation to the Sharpincpdm/cpdm phenotype has not been investigated. Here, we find increased integrin activity in keratinocytes from Tnfr1-/- Sharpincpdm/cpdm double knockout mice, which do not display chronic inflammation or proliferative dermatitis, thus suggesting that SHARPIN indeed acts as an integrin inhibitor in vivo. In addition, we present evidence for a functional contribution of integrin activity to the Sharpincpdm/cpdm skin phenotype. Treatment with an integrin beta 1 function blocking antibody reduced epidermal hyperproliferation and epidermal thickness in Sharpincpdm/cpdm mice. Our data indicate that, while TNF-induced cell death triggers the chronic inflammation and proliferative dermatitis, absence of SHARPIN-dependent integrin inhibition exacerbates the epidermal hyperproliferation in Sharpincpdm/cpdm mice.
Citation: Peuhu E, Salomaa SI, De Franceschi N, Potter CS, Sundberg JP, Pouwels J (2017) Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice. PLoS ONE 12(10): e0186628. https://doi.org/10.1371/journal.pone.0186628
Editor: Donald Gullberg, University of Bergen, NORWAY
Received: April 13, 2017; Accepted: October 4, 2017; Published: October 17, 2017
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: We gratefully acknowledge these funding sources: E.P. - Academy of Finland Postdoc Grant; S.S. - University of Turku Graduate School (Drug Research Doctoral Programme), Instrumentarium Foundation; N.d.F. - FinPharmaNet Doctoral Program, Intrumentarium Foundation, Orion Research Foundation, Finsk-Norska Medicinska Stiftelsen and the Magnus Ehrnrooth Foundation; C.S.P. and J.P.S. - National Institutes of Health (T32 DK07449-28 and R01-AR049288); J.P. - Finnish Cancer Institute, Instrumentarium Foundation and Academy of Finland. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: Dr. J.P. Sundberg has a sponsored research project with BIOCON LLC that is unrelated to this research. All other authors declare that they have no conflict of interest. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Mice that lack the multifunctional adaptor protein SHARPIN (Sharpincpdm/cpdm) display progressive multi-organ inflammation, which most prominently manifests with chronic eosinophilic hyperproliferative dermatitis characterized by skin thickening, keratinocyte hyperproliferation and severe inflammation [1,2], which are characteristics of psoriasiform dermatitis. Importantly, these pathological features in the skin are cell autonomous, as Sharpincpdm/cpdm grafts on wild type mice maintain the inflammatory phenotype . As an important component of the Linear Ubiquitination Assembly Complex (LUBAC) [4–6], SHARPIN regulates canonical NF-κB (Nuclear factor-kappaB) signaling downstream of several receptors, such as Tumor Necrosis Factor receptor (TNFR) , Toll-like receptor 2 , Toll-like receptor 3 , Interleukin1 receptor [4–6] and CD40 [5,10]. The role of NF-κB in the skin epidermis is controversial as some studies point to a pivotal role for NF-κB signaling in restraining epidermal growth (for example [11–13]), while other studies suggest that NF-κB signaling does not regulate development and differentiation of the epidermis (such as [14,15]). Importantly, crosses between Sharpincpdm/cpdm mice and Tnf-/- (Tnftm1Jods) or Tnfr1-/- (Tnfrsf1atm1Imx) mice almost fully rescue the inflammatory and dermatitis phenotypes of Sharpincpdm/cpdm mice [6,16,17], although other phenotypes were not rescued, such as abnormal leukocyte cell count, the absence of Peyer’s patches and absence of marginal zones in spleen [6,16]. Mechanistically, an increase in TNF-induced keratinocyte apoptosis in the absence of LUBAC-mediated NF-kB signaling drives the systemic inflammation and hyperproliferative dermatitis in Sharpincpdm/cpdm mice [6,16,17].
Integrins are the major cell adhesion receptors that mediate the interaction of a cell with the surrounding extracellular matrix, including the basement membrane. Binding of integrins to extracellular ligands triggers a conformational change in integrin structure that allows recruitment of a plethora of cellular factors resulting in activation of several signaling pathways . SHARPIN acts as an integrin inhibitor through binding to the integrin cytoplasmic domain, preventing the recruitment of integrin activating proteins and supporting the integrin inactive conformation . Functionally, SHARPIN-mediated integrin inhibition regulates cell adhesion and migration , as well as lymphocyte detachment during transmigration . Importantly, SHARPIN plays mutually exclusive roles in regulating integrins and LUBAC such that SHARPIN inhibits integrins independent of LUBAC .
Deregulated integrin activity is implicated in many human pathological conditions, including immune diseases, skin blistering, bleeding disorders, and cancer . In the skin, integrin expression is predominantly confined to the basal keratinocytes that anchor the epidermis to the basal lamina [22,23]. Transgenic mice overexpressing integrin beta 1 (Itgb1) in the suprabasal layer of the epidermis (Tg(Itgb1)0869Fmw) exhibit epidermal hyperproliferation, perturbed keratinocyte differentiation and skin inflammation , which resembles the Sharpincpdm/cpdm phenotype. Furthermore, integrin blocking therapies have been shown to alleviate psoriasis in mice  and in human patients . These data suggest that increased integrin activation in Sharpincpdm/cpdm mice may contribute to the dermatitis phenotype. We have previously demonstrated increased Itgb1 activity in the basal layer of the epidermis of Sharpincpdm/cpdm mice . However, whether this was due to the absence of SHARPIN-mediated integrin inhibition or secondary to the chronic inflammation-driven proliferative dermatitis has remained unclear.
Using Tnfr1-/- Sharpincpdm/cpdm double knockout mice we now present data suggesting that SHARPIN acts as an integrin inhibitor in vivo also in the absence of chronic inflammation. In addition, we demonstrate that Itgb1 inhibition with a function blocking antibody alleviates the excessive proliferation and apoptosis observed in Sharpincpdm/cpdm epidermis, but does not ameliorate the chronic and systemic inflammation in Sharpincpdm/cpdm mice, suggesting that increased integrin activity in the absence of SHARPIN exacerbates the hyperproliferative skin phenotype in Sharpincpdm/cpdm mice.
Itgb1 activation is elevated in basal keratinocytes from Tnfr1-/- Sharpincpdm/cpdm mice with normal epidermal thickness
We previously showed increased levels of active Itgb1 in the basal layer of the epidermis of Sharpincpdm/cpdm mouse skin , consistent with SHARPIN-mediated integrin inhibition. However, the increase in integrin activation could be secondary to the chronic inflammation-driven proliferative dermatitis in Sharpincpdm/cpdm mice. To address whether SHARPIN truly acts as an integrin inhibitor in vivo, we made use of the Tnfr1-/- Sharpincpdm/cpdm double knockout mice, in which the skin thickening (Fig 1A), chronic inflammation and dermatitis, typical to Sharpincpdm/cpdm mice, are absent [16,17]. This allowed us to investigate the role of SHARPIN in vivo without the consequences of increased Tnfr1-induced inflammation and apoptosis in Sharpincpdm/cpdm skin.
(A) Representative frozen skin sections from 6 weeks old Tnfr1+/+ Sharpin+/?, Tnfr1+/+ Sharpincpdm/cpdm, Tnfr1-/- Sharpin+/? and Tnfr1-/- Sharpincpdm/cpdm mice (n = 3 animals per genotype) stained for Keratin-14 (Krt14) and active Itgb1 (clone 9EG7). Scale bars represent 50 μm. Arrows indicate the basal cell layer. (B) Representative frozen skin sections from 6 weeks old Tnfr1-/- Sharpincpdm/cpdm mice stained for Krt14 (epidermis) and Itga6 (basal keratinocytes). Scale bars represent 10 μm. (C) Primary keratinocytes were isolated from Tnfr1-/- Sharpin+/?, Tnfr1-/- Sharpincpdm/cpdm and Tnfr1+/+ Sharpincpdm/cpdm mice and studied by flow cytometry. Live cells (FSC/SSC; left panel) were sorted for expression of Itga6 (CD49f) and lineage (Lin) markers CD31 and CD45 (central panel). Cell surface expression of active Itgb1 (CD29; clone 9EG7) was plotted (right panel) from the gated basal keratinocyte population (live, Itga6+Lin-). (D,E) Relative geometric mean fluorescence intensities of (D) active Itgb1 (n = 6, 5 and 4 animals per genotype) and (E) total Itgb1 (n = 4, 4 and 3 animals per genotype) cell surface staining on Tnfr1-/- Sharpin+/?, Tnfr1-/- Sharpincpdm/cpdm and Tnfr1+/+ Sharpincpdm/cpdm basal keratinocytes sorted as in (C). Numerical data are mean ± s.e.m.
Labeling skin sections from Tnfr1+/+ Sharpin+/?, Tnfr1+/+ Sharpincpdm/cpdm, Tnfr1-/- Sharpin+/? and Tnfr1-/- Sharpincpdm/cpdm mice with an antibody that specifically recognizes active Itgb1 [9EG7; ] confirmed the increased active Itgb1 levels in Tnfr1+/+ Sharpincpdm/cpdm epidermis  but also revealed elevated Itgb1 activity in Tnfr1-/- Sharpincpdm/cpdm basal keratinocytes despite normal skin thickness (Fig 1A).
To analyze this observation quantitatively and without the interference of high dermal Itgb1 activity (Fig 1A), we isolated keratinocytes from the epidermal layer of Tnfr1+/+ Sharpincpdm/cpdm, Tnfr1-/- Sharpin+/? and Tnfr1-/- Sharpincpdm/cpdm mice, and investigated Itgb1 activity specifically in basal cell layer keratinocytes that also express Integrin alpha 6 (Itga6, also known as CD49f; Fig 1B) (see Methods for details). In order to distinguish basal keratinocytes by flow cytometry, any residual cells expressing lineage markers for leukocytes [CD45, Protein Tyrosine Phosphatase, Receptor Type C (PTPRC)] or endothelial cells [CD31, Platelet And Endothelial Cell Adhesion Molecule 1 (PECAM1)] were gated out by flow cytometry, and cells expressing Itga6 were further analyzed for binding active Itgb1 (9EG7) or total Itgb1 (HMβ1–1) antibodies (Fig 1C). Quantification of these data showed that the amount of active Itgb1 on the surface of the double knockout Tnfr1-/- Sharpincpdm/cpdm basal keratinocytes was indeed significantly increased compared to Tnfr1-/- Sharpin+/? keratinocytes, although it was lower than in the hyperproliferative Tnfr1+/+ Sharpincpdm/cpdm cells (Fig 1D). The total Itgb1 levels were not significantly altered even though total Itgb1 levels seem elevated in Tnfr1+/+ Sharpincpdm/cpdm basal keratinocytes (Fig 1E). Importantly, FACS experiments with primary keratinocytes from four individual mice showed that staining with the 9EG7 antibody results in approximately 6 fold higher signals than isotype control (Panel A and B in S1 Fig). Altogether, these data suggest that increased Itgb1 activity in Sharpincpdm/cpdm mice is not solely due to chronic inflammation and proliferative dermatitis but that SHARPIN functions as an integrin inhibitor in vivo.
Itgb1 inhibition ameliorates the epidermal hyperproliferation phenotype in Sharpincpdm/cpdm mice
Given that SHARPIN indeed inhibits integrin activity in mouse epidermis (Fig 1), we hypothesized that the increased Itgb1 activity might contribute to the Sharpincpdm/cpdm dermatitis phenotype. Since epidermal Itgb1 depletion is lethal , Sharpincpdm/cpdm and age- and gender-matched control (Sharpin+/+ or Sharpin+/cpdm) mice were systemically treated with an Itgb1 function-blocking antibody or with PBS. Tnfr1-/- Sharpin+/? and Tnfr1-/- Sharpincpdm/cpdm mice were not included in this experiment as they display a normal skin phenotype. As expected, the epidermis of Sharpincpdm/cpdm mice was much thicker than in control treated mice (Fig 2A–2C). Interestingly, blocking Itgb1 function significantly reduced epidermal thickness in Sharpincpdm/cpdm, but not in control mice (Fig 2A–2C), without significantly affecting dermal thickness (Panel C in S1 Fig). From each animal the epidermal thickness was measured from five individual tissue sections with two measurements per skin section, thus minimizing the chances of recording experimental outliers. Importantly, plotting all data showed equal variation between measurements and a large difference between experimental conditions (Panel D in S1 Fig).
(A) Representative H&E-stained skin sections from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1 (Itgb1 function blocking antibody). Arrows indicate the epidermis. (B) Quantification of epidermal skin thickness from skin sections as depicted in (A) (n = 3 and 4 Sharpin+/? animals and n = 5 and 5 Sharpincpdm/cpdm animals per control and anti-Itgb1, respectively; five individual tissue sections were measured per animal with two measurements per skin section). Data from one out of two experiments with similar results are shown. (C,D) Representative frozen skin sections from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1, stained for Krt14 (C) or proliferation (Ki67) and basal keratinocytes (Itga6) (D). (E) Quantification of epidermal proliferation from skin sections as depicted in (D) (n = 2 Sharpin+/? and 3 Sharpincpdm/cpdm animals; 4–6 and 19–20 measurements per animal, respectively). (F, G, H) Sharpin+/? and Sharpincpdm/cpdm mouse primary keratinocytes were isolated from 6 weeks old mice and SHARPIN expression (F) and cell proliferation rate (G) were evaluated by western blotting and Cell Proliferation Reagent WST-1, respectively. (H) Relative proliferation of Sharpincpdm/cpdm and Sharpin+/? keratinocytes was quantified after 2 days incubation with 10 μg/ml anti-Itgb1 antibody or isotype IgG control (n = 7 animals per genotype). Scale bars are 50 (A) and 20 μm (C,D). All numerical data are mean ± s.e.m.
Staining for the proliferation marker Ki67 indicated that inhibition of Itgb1 in Sharpincpdm/cpdm mice reduced the number of proliferating epidermal cells (Fig 2D and 2E), suggesting that Itgb1 inhibition reduces keratinocyte proliferation. To determine if the reduced keratinocyte proliferation is an autonomous effect of Itgb1 inhibition, we cultured Sharpincpdm/cpdm and Sharpin+/? primary mouse keratinocytes, isolated from 6 weeks old mice (Fig 2F and 2G; Panel E in S1 Fig), and evaluated the effect of either Itgb1 function blocking antibody or isotype control antibody on cell proliferation (Fig 2H). While Sharpincpdm/cpdm keratinocytes demonstrated comparable or even higher proliferation rate than Sharpin+/? cells in vitro (Fig 2G), integrin inhibition with the Itgb1 function blocking antibody significantly reduced both Sharpincpdm/cpdm and Sharpin+/? primary keratinocyte proliferation (Fig 2H), showing that the Itgb1 blocking antibody inhibits keratinocyte proliferation autonomously and suggesting that amelioration of the chronic proliferative dermatitis (Fig 2A–2C) is, at least partly, linked to decreased keratinocyte proliferation.
Itgb1 inhibition does not reduce the chronic inflammation in Sharpincpdm/cpdm mice
Chronic inflammation in Sharpincpdm/cpdm mice is characterized by enhanced infiltration of immune cells to many tissues including the skin [1,29], as well as increased numbers of white blood cells in the peripheral blood . Interestingly, leukocyte (CD45+; Fig 3A and 3B), macrophage (F4/80+; Fig 3C and 3D) and mast cell (Toluidine blue+; Fig 3E and 3F) infiltration into the dermis of Sharpincpdm/cpdm mice was not affected by the Itgb1 function-blocking antibody, indicating that inflammation was not reduced. Also, the increased numbers of white blood cells in the peripheral blood of Sharpincpdm/cpdm mice remained elevated or even increased further upon Itgb1 inhibition (Panel A in S2 Fig), further suggesting that the chronic inflammation in Sharpincpdm/cpdm mice does not depend on Itgb1.
(A, C, and E) Representative skin sections from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1 antibody, and stained for (A) leukocytes (CD45), (C) macrophages (F4/80) and Krt14, or (E) mast cells (Toluidine blue). Nuclei were stained with DAPI (A, C), red arrows indicate toluidine blue positive mast cells (E). Scale bars represent 20 μm (A, C) and 50 μm (E). (B, D, and F) Quantification of (B) dermal leukocyte infiltration (n = 3 or 4 animals; 10–18 measurements per animal), (D) dermal macrophage infiltration (n = 3 or 4 animals; 9–19 measurements per animal) and (F) mast cell infiltration (amount of mast cells/skin section; n = 4 or 5 animals with 10 measurements per animal) from skin sections as depicted in (A, C, and E). Numerical data are mean ± s.e.m.
Elevated keratinocyte apoptosis in Sharpincpdm/cpdm mouse skin is partially rescued by Itgb1 inhibition
While the chronic proliferative dermatitis of Sharpincpdm/cpdm mice fully depends on TNF-induced keratinocyte apoptosis [6,16,17], Itgb1 antibody treatment did not affect the previously reported  elevation of Tnf expression levels in the Sharpincpdm/cpdm skin (Panel B and C in S2 Fig), suggesting that altered Tnf expression levels are not mediating the effects of the Itgb1 antibody treatment. However, systemic Itgb1 antibody treatment reduced the increased apoptosis in Sharpincpdm/cpdm epidermis (Fig 4A and 4B). Since Tnf levels remained high despite Itgb1 antibody treatment (Panel B and C in S2 Fig), the reduction in apoptotic cell numbers could rather be related to the decreased proliferation as keratinocyte apoptosis balances cell proliferation to control epidermal thickness . These data suggest that inhibition of integrin activity in Sharpincpdm/cpdm mice with an anti-Itgb1 antibody ameliorates the chain reaction leading to hyperproliferative dermatitis in the absence of SHARPIN.
(A) Representative frozen skin sections from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1, stained for apoptotic cells [cleaved caspase-3 (Casp3)]. Red arrows indicate cleaved caspase-3 positive apoptotic cells. Scale bars represent 20 μm. (B) Quantification of epidermal apoptosis from skin sections as depicted in (A) (n = 4 Sharpin+/? and 3 Sharpincpdm/cpdm animals; 13–18 measurements per animal). Numerical data are mean ± s.e.m.
The multifunctional adaptor protein SHARPIN regulates integrins [19,20] and TNF-induced NF-kB signaling [4–6], but whether defective integrin regulation also contributes to the Sharpincpdm/cpdm skin phenotype has remained unknown . This study now demonstrates that Itgb1 inhibition in Sharpincpdm/cpdm mice significantly reduces epidermal proliferation and apoptosis, suggesting a modulatory role for integrins in the chronic proliferative dermatitis phenotype.
Through direct interaction with the integrin cytoplasmic domain SHARPIN inhibits integrin activity, which plays a role in cell adhesion and migration [19,20]. Consistently, increased levels of active Itgb1 were observed in the epidermis of Sharpincpdm/cpdm mice . However, as Sharpincpdm/cpdm mice suffer from chronic proliferative dermatitis, it has remained unclear whether increased integrin activity in the Sharpincpdm/cpdm epidermis is due to lack of SHARPIN or merely a side effect of the dermatitis. Using Tnfr1-/-Sharpincpdm/cpdm double knock out mice  we now report that Itgb1 activity is also increased in basal Tnfr1-/- Sharpincpdm/cpdm keratinocytes in the absence of proliferative dermatitis, supportive of a direct role for SHARPIN in integrin inhibition in vivo. The Tnfr1-/- Sharpincpdm/cpdm mice and other double knock out mice that rescue the Sharpincpdm/cpdm inflammatory phenotype [6,16,17] are excellent tools to dissect which in vivo functions of SHARPIN are a side effect of the inflammatory phenotype and which phenotypes are independent of the inflammation. In addition, as Tnfr1-/- Sharpincpdm/cpdm mice have a considerably longer life span than Sharpincpdm/cpdm mice, which need to be sacrificed by 8 weeks due to severe skin inflammation, these double knock out mice provide means to address whether SHARPIN has important functions later in development and in the development of diseases, such as cancer.
Sharpincpdm/cpdm mice display skin thickening (hyperkeratosis), increased keratinocyte proliferation and apoptosis, as well as increased inflammatory cell infiltration [1,2,29]. The phenotype has been shown to originate from defective NF-kB signaling and increased apoptosis induction in response to Tnfr1 activation in keratinocytes, as crosses between Sharpincpdm/cpdm mice and Tnf-/- or Tnf1r-/- mice fully rescued the chronic proliferative dermatitis [6,16,17]. Transgenic mice with suprabasal expression of Itgb1 displayed similar chronic proliferative dermatitis with deregulated keratinocyte proliferation and skin inflammation, although apoptosis was not significantly affected . In Sharpincpdm/cpdm mice, however, the increased Itgb1 activity was primarily observed in basal keratinocytes negative for Keratin-10 or involucrin expression . Our study now shows that inhibition of Itgb1 in Sharpincpdm/cpdm mice decreases both keratinocyte proliferation and apoptosis. Importantly, inflammation was not affected, suggesting that the cause of the alleviated phenotype is different from that in the aforementioned Tnf-/- Sharpincpdm/cpdm and Tnfr1-/- Sharpincpdm/cpdm double knockout mice. Thus, while Tnf-induced keratinocyte death drives chronic proliferative dermatitis in Sharpincpdm/cpdm mice, increased Itgb1 activity appears to exacerbate the hyperproliferative skin phenotype, consistent with the important role of Itgb1 in keratinocyte proliferation .
Recently, SHARPIN was also shown to bind and inhibit the T cell receptor (TCR) in a LUBAC-independent fashion, thereby playing an intrinsic role in the generation of regulatory T cells (Treg cells) . Importantly, transfer of wild type Treg cells into Sharpincpdm/cpdm mice considerably alleviated their systemic inflammation . These and our data together show that, though chronic inflammation in Sharpincpdm/cpdm mice is triggered by Tnf-mediated apoptosis [6,16,17], other regulatory roles of SHARPIN contribute to different aspects of the complex Sharpincpdm/cpdm phenotype.
In summary, our data suggest that Sharpin functions as an integrin inhibitor in vivo and that integrins contribute to the chronic proliferative dermatitis phenotype in Sharpincpdm/cpdm mice. We therefore postulate that different aspects of the complex Sharpincpdm/cpdm phenotype, also in tissues other than skin, could be a result of SHARPIN’s ability to regulate several proteins.
Materials and methods
The C57BL/KaLawRij-Sharpincpdm/RijSunJ mouse strain (Stock No: 007599 |) with a spontaneous mutation leading to the complete loss of SHARPIN protein [1,2] was acquired from The Jackson Laboratory (Bar Harbor, ME). The colony was maintained in heterozygote breeding and genotyped for the Sharpincpdm/cpdm mutation to obtain Sharpincpdm/cdpm homozygous mice and littermate control mice (Sharpin+/+ or Sharpin +/cpdm) for experiments. DNA was extracted with KAPA Mouse Genotyping Kit (KK7302) and the Sharpincpdm mutation detected using 40x genotyping assay mix (TaqMan SNP Genotyping Assays, 5793982, Applied Biosystems) and TaqMan Universal PCR Master Mix. Tnfrsf1atm1Imx Sharpincpdm/cdpm mice  (Tnfrsf1atm1Imx Stock No: 003242; hereafter called Tnfr1-/-), obtained from Prof. H. Walczak, were also maintained in heterozygote breeding. PCR amplification was used for detection of the deletion in Tnfr1 gene in somatic DNA. Age-matched mice were used in all the experiments described here. As both male and female Sharpincpdm/cdpm mice develop the same symptoms and we have to perform heterozygous breedings, both genders were used in the experiments.
Mice were housed in standard conditions (12-h light/dark cycle) with food and water available ad libitum. The viability, clinical signs and behaviour of the mice were monitored daily. For euthanasia, cervical dislocation was used in conjunction with CO2. All animal experiments were ethically assessed and authorised by the National Animal Experiment Board and in accordance with The Finnish Act on Animal Experimentation (Animal licence numbers 7522/04.10.03/2012, ESAVI-5588-04.10.07–2014, ESAVI-9339-04.10.07–2016) and The Jackson Laboratory Animal Care and Use Committee (approval number 07005).
Antibody treatment of mice
Five pairs of 5-week old gender-matched Sharpincpdm/cpdm and Sharpin+/? littermates were injected intraperitoneally with 0.1 ml of 0.05 mg/ml function-blocking anti-mouse ITGB1 antibody (LEAF™ purified anti-mouse CD29 Armenian hamster IgG (clone HMB1-1, Biolegend) or PBS twice a week. Mice were injected for a total of 3 weeks (6 injections) and then euthanized by CO2 asphyxiation. Complete necropsies were performed as described . Skin was collected in Fekete’s acid-alcohol-formalin. Dorsal skin was also collected in 4% paraformaldehyde for immunofluorescence or fresh frozen for IHC. Blood was collected in EDTA tubes for analysis using the ADVIA 120 Hematology System (Siemens Healthcare Global). Four pairs of 5-week old littermates injected with 0.1 ml of 0.5 mg/ml anti-mouse CD29 antibody or Armenian hamster IgG negative control antibody (Biolegend) showed highly similar results.
The antibodies used in this study are LEAF™ purified Anti-mouse CD29 Armenian hamster IgG (Biolegend; HMB1-1), Armenian hamster IgG negative control (Biolegend), Itga6 (Serotec; NKI-GoH3; 1:200 in immunohistochemistry of frozen sections (IHC-Fr), cleaved Casp3 (Cell Signaling; 5A1E; 1:400 IHC-Fr), Ki67 (Abcam; ab66155; 1:300 IHC-Fr), CD45 (PTPRC) (BD Pharmingen; 30-F11; 1:250 in IHC-Fr, 1:200 in FACS), F4/80 (CiteAb; MF48020; 1:50 in IHC-Fr), Krt14 (Biosite; PRB-155P; 1:600 in IHC-Fr), Tnf (Abcam; ab6671; 1:200 in IHC-Fr), CD31 (PECAM1) (Biolegend; 1:50 in FACS), active Itgb1 (BD Pharmingen; 9EG7; 1:100 in IHC-Fr, 1:50 in FACS), total Itgb1 (CD29-Alexa Fluor 647) (Biolegend; HMβ1–1; 1:50 in FACS), CD49f (Itga6) (Biolegend; GoH3; 1:20 in FACS), SHARPIN (Proteintech; 1:1000 WB), Krt14 (Covance; 1:1000 WB), Vimentin (D21H3, Cell Signalling; 1:1000 WB), GAPDH (5G4 Mab 6C5, Hytest; 1:1000 WB), DyLight 680- or 800-conjugated IgGs (Thermo Scientific; 1:5000 WB) and AlexaFluor 488/568/647-conjugated secondary Igs (Invitrogen; 1:300 in IHC-Fr and FACS).
O.C.T. (Sakura) embedded frozen skin sections were fixed (4% paraformaldehyde), quenched (100mM glycine in PBS), and blocked/permeabilized (2% BSA, 0.1% Triton X100 in PBS). Samples were labeled with primary antibodies (1 hour at RT in 2% BSA in PBS), washed three times and labeled with fluorescent secondary antibodies. After washes, samples were mounted in Vectashield mounting medium (Vector Laboratories) and viewed using a 20x objective on either a 3i Marianas Spinning disk confocal microscope (Intelligent Imaging Innovations) or Zeiss Axiovert 200M with Yokogawa CSU22 spinning disk confocal microscope unit with Hamamatsu Orca ER CCD camera (Hamamatsu Photonics K.K.). Images were processed and quantified using Fiji image analysis software .
Isolation and culture of mouse primary keratinocytes
6–8 weeks old littermate mice were sacrificed, dorsal skin was shaved and a piece of dorsal skin (1.5 cm x 1.5 cm) was harvested in cold PBS. Tissue was cut further to smaller pieces, rinsed with Hank’s Balanced Salt Solution without Ca2+ and Mg2+ (HBSS; Sigma), and incubated overnight in 0,25% porcine trypsin (Sigma) in HBSS at +4°C on a shaker. Next day, the dermis was removed with forceps, the remaining epidermis was chopped with a scalpel and incubated in 0.2% Collagenase XI (Sigma) at 37°C incubator for 30 min. Keratinocytes were dissociated from the tissue by pipetting the suspension every 5–10 minutes. Keratinocytes were filtered through a cell strainer (70 μm; BD Biosciences). Finally, cells were incubated with 20 U/ml DNase I (Roche) for 5 min on ice and spun down.
Primary keratinocytes were cultured on a 6-well plate coated with 20 μg/ml Collagen Type I from rat tail (Merck Millipore) in FAD medium [Dulbecco’s Modified Eagle’s Medium:Ham’s F12 Nutrient Mixture (GIBCO) 3.5:1.1] supplemented with 10% fetal bovine serum (FBS) (chelated using Bio-Rad Chelex® 100 Chelating Resin), 5 μg/ml insulin, 100 pM choleratoxin, 10 ng/ml epidermal growth factor (EGF), 100 U/ml sodium pyruvate, 100 μg/ml penicillin-streptomycin and 0.5 μg/ml hydrocortisone (all from Sigma Aldrich if not otherwise mentioned) in 5% CO2 and 32°C.
Keratinocyte proliferation assay
Equal amounts of primary mouse keratinocytes were plated on 96-well plates coated with 20 μg/ml rat tail Collagen Type I. One day after seeding, the keratinocytes were treated with 10 μg/ml anti-Itgb1(Anti-mouse CD29 Armenian hamster IgG) or isotype IgG control antibody. Theamount of cells) in each well was quantified with Cell Proliferation Reagent WST-1 (Roche, Sigma Aldrich) according to the manufacturer’s instructions and a BioTek Synergy H1 Hybrid Multi-Mode Microplate Reader. The relative proliferation was calculated by normalizing the amount of cells in each time point to day zero (Fig 2G) or by subtracting the amount of cells at day zero from the value at day two (corrected cell amount), followed by normalizing to the corrected cell amount of IgG control. A minimum of two wells per keratinocyte primary cell line were analysed per time point.
The active conformation specific Itgb1 antibody (clone 9EG7, BD Pharmingen) was concentrated with an Amicon® Ultra filter centrifugation and directly conjugated to Alexa Fluor 647 with APEX® antibody labelling kit (Thermo Fischer Scientific). Cells were fixed with 2% paraformaldehyde in PBS for 10 min at RT, after which they were centrifuged and washed with cold Tyrodes buffer (10 mM Hepes-NaOH pH 7.5, 137 mM NaCl, 2.68 mM KCl, 0.42 mM NaH2PO4, 1.7 mM MgCl2, 11.9 mM NaHCO3, 5 mM glucose). The cells were stained with directly fluorochrome-conjugated antibodies [CD31-Pacific Blue (PECAM1), CD45-Pacific Blue (PTPRC), CD49f-488 (Itga6, Clone: GoH3) (all from BioLegend), and CD29-AlexaFluor 647 or active Itgb1 (clone 9EG7) -647] in 100 μl Tyrodes for 30 min RT, washed with Tyrodes and resuspended in PBS. Filtered cells were analysed using BD LSRFortessa (BD Biosciences) and FlowJo analysis software (Tree Star).
Protein content in primary mouse keratinocytes was analysed by immunoblotting using standard western blotting techniques and the Odyssey LICOR imaging system.
S1 Fig. Experiments in (C-E) show data from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1 (Itgb1 function blocking antibody).
(A,B) FACS analysis (A) and quantification (B) of active Itgb1 (10 μg/ml Rat Anti-Mouse CD29, Clone 9EG7) labelling specificity compared to isotype IgG control (10 μg/ml Rat IgG2a, κ isotype control) in primary mouse keratinocytes using AlexaFluor647 conjugated anti-rat secondary antibody. Samples were analyzed by flow cytometry at SSC/FSC and APC-A channels, and background fluorescence was subtracted before normalization to Isotype IgG (mean +/-SEM; n = 4 mice). (C) Quantification of dermal skin thickness from skin sections similar to those depicted in Fig 2A (n = 4 or 5 animals with 10 measurements per animal). (D) Dot plot of all individual measurements of epidermal skin thickness from skin sections similar to those depicted in Fig 2A (five individual tissue sections were measured per animal with two measurements per skin section). The plot in Fig 2B shows averages per animal. (E) Krt-14 (marker for keratinocytes), vimentin (marker for fibroblasts) and GAPDH expression levels in keratinocytes isolated from Sharpin+/+, Sharpin+/- and Sharpincpdm/cpdm mice, as well as mouse embryonic fibroblast (MEF). Keratinocyte isolation purity was evaluated by measuring the Krt14/vimentin ratio, normalized to GAPDH, with MEFs serving as a control fibroblast cell line. Molecular weight markers are indicated (KDa). All numerical data are mean ± s.e.m. Scale bars represent 20 μm.
S2 Fig. Blocking Itgb1 activity does not affect inflammation or Tnf levels in Sharpincpdm/cpdm mice.
Experiments show data from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1 (Itgb1 function blocking antibody). (A) Quantification of immune cell populations from peripheral blood. WBC, white blood cells; LUC, large unstained cells. (B,C) Representative skin sections stained for Tnf and nuclei (DAPI) (B) and quantification of dermal Tnf levels (C) (n = 2 and 4 Sharpin+/? animals in control and anti-CD29 group, respectively, and 5 in each Sharpincpdm/cpdm group, 10–40 measurements per animal). Dotted line marks the basement membrane. All numerical data are mean ± s.e.m. Scale bars represent 20 μm.
We thank J. Jukkala, P. Laasola, L. Lahtinen and J. Siivonen for technical assistance and the Cell Imaging Core at Turku Centre for Biotechnology for assistance with flow cytometry. M. Khan and Turku Centre for Disease Modelling (TCDM) for assistance in mouse genotyping. H. Walczak (University College London) is acknowledged for generously sharing the Tnfr1-/- Sharpincpdm/cpdm mice.
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