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Paleoproteomics of the Dental Pulp: The plague paradigm

  • Rémi Barbieri ,

    Contributed equally to this work with: Rémi Barbieri, Rania Mekni

    Roles Data curation, Writing – review & editing

    Affiliation Aix-Marseille Université, URMITE, CNRS, Faculté de Médecine IHU Méditerranée-Infection, Marseille, France

  • Rania Mekni ,

    Contributed equally to this work with: Rémi Barbieri, Rania Mekni

    Roles Data curation, Writing – review & editing

    Affiliation Aix-Marseille Université, URMITE, CNRS, Faculté de Médecine IHU Méditerranée-Infection, Marseille, France

  • Anthony Levasseur,

    Roles Formal analysis

    Affiliation Aix-Marseille Université, URMITE, CNRS, Faculté de Médecine IHU Méditerranée-Infection, Marseille, France

  • Eric Chabrière,

    Roles Formal analysis

    Affiliation Aix-Marseille Université, URMITE, CNRS, Faculté de Médecine IHU Méditerranée-Infection, Marseille, France

  • Michel Signoli,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Aix-Marseille Université, EFS-CNRS, Marseille, France

  • Stéfan Tzortzis,

    Roles Data curation, Writing – review & editing

    Affiliation Aix-Marseille Université, EFS-CNRS, Marseille, France

  • Gérard Aboudharam,

    Roles Conceptualization, Writing – review & editing

    Affiliation Aix-Marseille Université, URMITE, CNRS, Faculté de Médecine IHU Méditerranée-Infection, Marseille, France

  • Michel Drancourt

    Roles Conceptualization, Supervision, Writing – review & editing

    Michel.Drancourt@univ-amu.fr

    Affiliation Aix-Marseille Université, URMITE, CNRS, Faculté de Médecine IHU Méditerranée-Infection, Marseille, France

Paleoproteomics of the Dental Pulp: The plague paradigm

  • Rémi Barbieri, 
  • Rania Mekni, 
  • Anthony Levasseur, 
  • Eric Chabrière, 
  • Michel Signoli, 
  • Stéfan Tzortzis, 
  • Gérard Aboudharam, 
  • Michel Drancourt
PLOS
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Abstract

Chemical decomposition and fragmentation may limit the detection of ancient host and microbial DNA while some proteins can be detected for extended periods of time. We applied paleoproteomics on 300-year-old dental pulp specimens recovered from 16 individuals in two archeological funeral sites in France, comprising one documented plague site and one documented plague-negative site. The dental pulp paleoproteome of the 16 teeth comprised 439 peptides representative of 30 proteins of human origin and 211 peptides representative of 27 proteins of non-human origin. Human proteins consisted of conjunctive tissue and blood proteins including IgA immunoglobulins. Four peptides were indicative of three presumable Yersinia pestis proteins detected in 3/8 dental pulp specimens from the plague-positive site but not in the eight dental pulp specimens collected in the plague-negative site. Paleoproteomics applied to the dental pulp is a new and innovative approach to screen ancient individuals for the detection of blood-borne pathogens and host inflammatory response.

Introduction

The discovery and the characterization of microbes in ancient environmental and human specimens expanded the knowledge about the evolution of microbiota and pathogens and rose new paradigms concerning the dynamics of deadly epidemics [1]. New insights into bacteria and archaea constituting past microbiota have been gained notably by analyzing ancient dental calculus microbiota [24] and digestive tract microbiota [5]. Furthermore, an expanding knowledge of the evolution of pathogens such as Yersinia pestis [6, 7], Mycobacterium tuberculosis and Mycobacterium leprae [8] and variola [9] helped reconstitute the dynamics of past devastating epidemics caused by these pathogens.

These paleomicrobiological studies have been mainly based on the classical detection of DNA sequences by using targeted PCR-sequencing [10] 16S rRNA gene PCR-sequencing [11], 16S rRNA gene PCR-NGS [12] and DNA-array-based capture and NGS [7, 13]. Later studies culminated in the reconstitution of the complete genome of ancient strains of Y. pestis in Bronze Age individuals [6] and historical plague pandemic victims [7, 14, 15], Vibrio cholerae [16], Mycobacterium tuberculosis [8], Borrelia burgdorferi [17] and Treponema denticola [18]; and host-associated viruses [19] including smallpox virus from human specimens buried for 300 years [9].

While ancient DNA decay may limit the fate of discovery of ancient microbes [20], proteins have been shown to resist alteration for hundreds of thousands of years [21, 22]. For example, no less than 126 different proteins were retrieved from the femur of a 43,000-year-old mammoth preserved in permafrost in Siberia [23]. For instance, mass spectrometry analyses of proteins resolved the question of the sheep and cattle sources of the 5,300-year-old Tyrolean Iceman’s clothes [24]. As for the discovery of microbes, this approach has been limited to the study of the dental calculus [25, 26].

We tested the hypothesis that microbes could leave identifiable protein signatures in ancient dental pulp by using ancient plague as a paradigm.

Results

Ancient dental pulp contains peptides

In a first step, dental pulp was collected from 16 teeth collected in 16 individuals buried in two different archaeological sites in France. These sites chosen were one negative control site without any historical, anthropological and microbiological evidence for plague (Nancy, dated 1793–1795); and one positive control site with anthropological and historical pieces of evidence of plague confirmed by previous PCR-sequencing of Y. pestis (Le Delos, dated 1720–1721) [27]. After protein extraction and purification, we observed that the concentration of proteins varied from 0.08 to 1.5 g/L. Then, mass spectrometry analysis of the 16 teeth yielded a total of 650 peptides ≥ 10 residues. The analysis of these peptides identified a total of 57 proteins in addition to trypsin used for peptidic digestion and keratins discarded as probable contaminants, whereas negative controls run in parallel yielded no peaks.

Ancient dental pulp peptides identifying proteins of human origin

In a second step, we observed that 439 peptides were indicative of proteins of human origin, retrieved in 6/16 teeth under investigation. These peptides were indicative of a total of 30 different human proteins, comprising blood proteins (10) including immunoglobulins; connective tissue proteins (6) including collagen 1 and collagen 2; and proteins of other sources (14) like orexin and dermicin [28] (Table 1). Moreover, 14/30 proteins derived from the paleoproteomic analysis of the ancient dental pulp proteomes had been previously detected in modern-day dental pulp [29]. Keratin type 1 was assigned as a skin contaminant as keratin type 2 had been previously interpreted as a skin contamination in the modern-day dental pulp proteome [29]. In addition, five proteins detected in ancient dental pulp but not in modern-day dental pulp are deriving from blood, comprising lipocalin, immunoglobulin A and the coagulation factor.

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Table 1. List of human proteins (except for keratins, interpreted as contaminants) identified by paleoproteomic investigations of 16 ancient dental pulp specimens collected in two archeological sites, France.

https://doi.org/10.1371/journal.pone.0180552.t001

Ancient dental pulp peptides identifying Y. pestis proteins.

A total of 211 peptides of bacterial origin were identified in eight ancient dental pulp specimens (S1 Table). Four peptides detected in three different individuals S16, S22 and S23 in the positive plague Delos site were found to be representative of three different proteins i.e. Blast comparisons showed that EIR43209.1, WP_002222869.1 and WP_002210283.1 exhibited 100% identity and 100% coverage with Yersinia spp. genome (Table 2). In particular, one peptide retrieved twice from individual S22 was found to Blast only with 100% identity and 100% coverage with Yersinia pseudotuberculosis and Y. pestis. No peptide similar to Y. pestis proteome was retrieved from any of the eight specimens collected in the negative control site. According to the Pearson’s chi-squared test, the probability to find peptides from Yersinia exclusively in the Delos site is only equal to a P value of 0.05466394 with a X2 indicator of 3.69230769.

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Table 2. List of four peptides retrieved from three individuals in a documented 18th century plague site, France; exhibiting 100% identity and 100% coverage (Blast on NCBI) with at least Y. pestis.

* This peptide was found twice in the S22 individual.

https://doi.org/10.1371/journal.pone.0180552.t002

Discussion

We applied paleoproteomics to the dental pulp collected from buried individuals in order to develop a new diagnostic approach for ancient infectious diseases, using plague as an illustrative situation. Data here reported indicate that four peptides corresponding among others to Y. pestis proteins have been detected in three individuals exhumed from a documented 18th century plague site in France, while no such peptide was detected in a negative control 18th century site in France. In particular, one of these four peptides found twice in one individual yielded significant blast results only with Y. pestis and Y. pseudotuberculosis, which are undistinguishable when using this approach. In light of the historical, archeological and anthropological data from the archeological site of Le Delos [27] in which the presence Y. pestis was already confirmed by F1 antigen detection and suicide PCR [30, 31], we interpreted these four peptides as indicative of Y. pestis in these three individuals who died during the plague epidemic of 1720–1722.

Twenty years ago, we introduced the dental pulp as a suitable specimen on which to base the DNA detection of ancient blood-borne pathogens, chiefly Y. pestis [1, 10]. Following these pioneering works, the dental pulp has been used to recover the complete Y. pestis genome from individuals of the Bronze Age [6], medieval individuals [14] and 18th century individuals [13]. Appropriate PCR-sequencing strategies also enabled us to retrieve specific DNA sequences in ancient dental pulp specimens, including the trench fever agent Bartonella quintana [32], the typhus agent Rickettsia prowazekii [33] and the typhoid fever agent Salmonella enterica Typhi [34].

Here, we report that the dental pulp preserves ancient peptides detectable by paleoproteomics. In this tissue, ancient peptides can be detected by highly sensitive chromatographic methods hence eliminating any amplification process and limiting potential in-laboratory contamination. Accordingly, recovered host proteins included conjunctive tissue proteins and a few blood proteins such as immunoglobulins. This expands the spectrum of retrievable inflammatory proteins previously generated by the paleoproteomic analysis of the human dental calculus [35]. Obviously, the dental calculus and the dental pulp were readily available contrary to brain tissue in which proteins had been previously analyzed in the Tyrolean Iceman [36]. The fact that immunoglobulins have been easily detected in ancient dental pulp suggests that studying ancient dental pulp proteome may give information on both the pathogen and the host inflammatory response and be used for direct and indirect serological diagnoses. This may connect with the seroepidemiology of past infections and past conditions currently diagnosed by the detection of specific immunoglobulins and other protein markers.

Not only host proteins could be recovered but also pathogen-specific ones as demonstrated in this report. As for Y. pestis, the century-long preservation of the F1 antigen in the dental pulp has been previously reported [30]. Accordingly, the pathogen and its antigens were detected in the dental pulp by using immuno-PCR [37]. Indeed, the detection of specific protein sequences is a step towards the detection of ancient pathogens. This could be of particular interest for the detection of RNA virus as the conservation of viral RNA in ancient specimens is poorly documented apart from an exceptional observation of the Ancient Northwest Territories cripavirus in 700-year-old caribou frozen feces [19].

Paleoproteomics of the dental pulp opens a new area of research in paleopathology, allowing for the diagnosis of both a blood-borne pathogen and the host inflammatory response to this pathogen.

Materials and methods

Ancient teeth collection

A total of 16 teeth were collected from individuals buried in two different sites in France. These teeth have been further preserved in the Regional ostéothèque, Marseille Medical School, Marseille, France.

The specimen numbers used for this study are:

For Nancy site (Usine Berger Levrault, 1794,1795): 1, 2, 3, 4, 5, 6, 7, 8

For Le Délos site (Martigues, 1720,1721): 6, 8, 10, 13, 16, 20, 22, 23

These teeth have been further preserved in the Regional ostéothèque DRAC-PACA, Marseille Medical School, North sector, Batiment A—CS80011, Bd Pierre Dramard, 13344 MARSEILLE Cedex 15, France, under the direction of Yann Ardagna and Emeline Sperandio.

No permits were required for the described study, which complied with all relevant regulations.

Eight teeth were collected from eight individuals found at the site of the Berger-Levrault factory, Nancy, used as a plague-negative control site. In 2010, rehabilitation works allowed for the discovery of a vast graveyard of the eighteenth and nineteenth centuries, part of which was the subject of an archaeological excavation operation. Historical archives indicated that this cemetery had been established ex nihilo in 1732; and that the excavated area dated from 1779–1842. The excavated area was located along the walls of the cemetery fence and comprised wide-trench burials for burial beds hosting several hundred individuals. Anthropological studies and archives indicated that they were French soldiers who died in a hospital setting between June 1793 and February-March 1795 [38]. Anthropological data and historical sources indicated no history of plague.

Then, eight teeth were collected from eight individuals buried in two ditches in a mass grave excavated in 1994 in the Delos site, Martigues, used as the plague-positive control site [26]. A total of 39 skeletons were exhumed (21 adults and 18 immature individuals). Historical sources indicated this was an emergency burial site dated from 1720, at a time when plague swept over Provence; and formally confirmed as a plague site by PCR-sequencing [1, 10]. Accordingly, the Delos site is one of the best characterized plague mass graves in Southern France. The dental pulp was extracted individually using new disposable instruments as previously described [1]. Extirpated dental pulps were stored no more than five days at 20°C prior to protein extraction.

Dental pulp protein extraction

Proteins were extracted from every dental pulp specimen as previously described by Cappellini [39] with minor modifications. Briefly, the dental pulp was first powdered by sonication. Protein extraction was performed on 1.3 mg of dental pulp powder suspended in 200 μL of a 0.5 M EDTA (pH 8.01) solution incubated overnight at 4°C. After 15 min of centrifugation at maximum speed on a bench-top centrifuge at 4°C, the supernatant (referred as the EDTA fraction) was stored at -20°C. Pellets were washed twice with 200 μL of distilled water, then re-suspended in 100 μL of a 50 mM ammonium bicarbonate solution (pH 7.40) and incubated 48 h at 75°C. The specimen was then centrifuged for 15 min at maximum speed at 4°C and the supernatant (referred as bicarbonate fraction) was collected and stored at -20°C. Pellets were collected separately and re-suspended in TS buffer (urea 7M, thiourea 2M, CHAPS 4%) and incubated at 30°C for 4 h. After centrifugation for 15 min at maximum speed, the supernatant (referred as TS Fraction) was stored at -20°C. All fractions (EDTA, bicarbonate and TS) were dialyzed using Slide-ALyzer Dialysis Cassettes 2K MWCO (Pierce Biotechnology, Rockford, USA) with 2L of dialysis solution (50 mM ammonium bicarbonate Ph 7.40, urea 1M solution) for 4h. Then the dialysis solution was changed and a new dialysis was performed overnight. Dialyzed fractions were collected and quantified by Bradford Assay using Coomassie (Bradford). This mixture was reduced by 1 h-incubation at 60°C with 5 mM final concentration of dithiothreitol. The reduced cysteines were then alkylated by a 45 min-incubation in the dark at room temperature in a 15 mM iodoacetamide solution. Final pH was adjusted between 7.40 and 7.60 using concentrated sodium hydroxide. Soluble proteins were reduced and alkylated with iodoacetamide. Alkylated proteins were digested using 0.5 μg of sequencing grade trypsin (overnight incubation at 37°C). The three fractions were washed and de-salted using Detergent Removal Procedure and stored at -20°C.

LC/MS analysis

For protein identification by LC-ESI-MS/MS, purified proteins were digested with trypsin and trypsin digests were analyzed using a nanoAcquity UPLC system connected to a Synapt G2Si Q-TOF ion mobility hybrid spectrometer. Peptides were eluted onto a trapping column (nanoAcquity UPLC 2G-V/M Trap 5μm Symmetry C18 180μm x 20mm, Waters) for concentration and desalting, at 10 μL/min of 99.9% water 0.1% formic acid and 0.1% acetonitrile 0.1% formic acid. Peptides were eluted on a C18 100 μm x 100 mm column (nanoAcquity UPLC 1.7μm BEH C18, Waters) and separated using a 100 minutes gradient (300 nl/min, 5 to 40% acetonitrile 0.1% formic acid). Data independent MS/MS monitoring (HDMSe) was performed in positive Resolution Mobility TOF mode. Capillary voltage was set to 3 kV, sampling cone to 40 V and source temperature to 90 degrees. MS range was 50–2000 m/z, Trap cell energy was 4 V, Transfer cell low energy was 5 V and high fragmentation energy was a 19–45 V ramp. Typical on-column specimen load was approximately 400 ng per specimen. Raw MS data was processed using PLGS 3.0.1 software. GFP lock mass correction was applied to all spectra. Processing thresholds were set as follow: low energy = 250 counts, elevated energy = 100 counts, intensity = 750 counts. The following workflow parameters were set for protein database searching: monoisotopic masses, 1+ minimum peptide charge, Trypsin, peptide and fragment automatic tolerances, 1 missed cleavage, carbamidomethyl C as fixed modification, deamination NQ and oxidation M as variable modification, 4% false discovery rate, 1 minimum peptide per protein, 1 minimum fragment ion matches per peptide, 3 minimum fragment ion matches per protein. NCBI and Swissprot online protein sequences were used for protein identification. All specimens MS datasets were compared to the entire Swissprot database (November 2014) and a concatenated NCBI database containing Homo sapiens, Yersinia and common environment contaminant sequences (November 2014). Keratines were excluded from the results. Proteins presenting one or more peptides were considered as identified.

Pearson’s chi-squared test

A pearson’s chi squared test was realized between the sample of Delos and the sample of Nancy to probe the probability that the repartition of Y. pestis peptides detected had nothing to do with chance. Values used were of dd l = 1 and P<0.1for the analysis.

Supporting information

S1 Table. List of bacterial peptides identified in ancient dental pulp specimens collected in two archeological sites, France.

https://doi.org/10.1371/journal.pone.0180552.s001

(DOCX)

References

  1. 1. Drancourt M, Raoult D (2005) Palaeomicrobiology: current issues and perspectives. Nat Rev Microbiol 3: 23–35. pmid:15608697
  2. 2. Warinner C, Rodrigues JF, Vyas R, Trachsel C, Shved N, Grossmann J et al. (2014) Pathogens and host immunity in the ancient human oral cavity. Nat Genet 46: 336–344. pmid:24562188
  3. 3. Warinner C, Speller C, Collins MJ (2015) A new era in palaeomicrobiology: prospects for ancient dental calculus as a long-term record of the human oral microbiome. Philos Trans R Soc Lond B Biol Sci 370: 20130376. pmid:25487328
  4. 4. Huynh HTT Huynh HT, Nkamga VD, Signoli M, Tzortzis S, Pinguet R, Audoly G et al. (2016) Restricted diversity of dental calculus methanogens over five centuries, France. Sci Rep, in press.
  5. 5. Appelt S, Armougom F, Le Bailly M, Robert C, Drancourt M (2014) Polyphasic analysis of a middle ages coprolite microbiota, Belgium. PLoS One 9: e88376. pmid:24586319
  6. 6. Rasmussen S, Allentoft ME, Nielsen K, Orlando L, Sikora M, Sjögren KG et al. (2015) Early divergent strains of Yersinia pestis in Eurasia 5,000 years ago. Cell 163: 571–582. pmid:26496604
  7. 7. Bos KI, Jäger G, Schuenemann VJ, Vågene ÅJ, Spyrou MA, Herbig A et al. (2015) Parallel detection of ancient pathogens via array-based DNA capture. Philos Trans R Soc Lond B Biol Sci 370: 20130375. pmid:25487327
  8. 8. Donoghue HD, Spigelman M, O'Grady J, Szikossy I, Pap I, Lee OY et al. (2015) Ancient DNA analysis—An established technique in charting the evolution of tuberculosis and leprosy. Tuberculosis (Edinb) 95 Suppl 1: S140–S144.
  9. 9. Biagini P, Thèves C, Balaresque P, Géraut A, Cannet C, Keyser C et al. (2012) Variola virus in a 300-year-old Siberian mummy. N Engl J Med 367: 2057–2059. pmid:23171117
  10. 10. Raoult D, Aboudharam G, Crubézy E, Larrouy G, Ludes B, Drancourt M et al. (2000) Molecular identification by “suicide PCR” of Yersinia pestis as the agent of medieval black death. Proc Natl Acad Sci U S A 97: 12800–12803. pmid:11058154
  11. 11. Tran-Hung L, Tran-Thi N, Aboudharam G, Raoult D, Drancourt M (2007) A new method to extract dental pulp DNA: application to universal detection of bacteria. PLoS One 2: e1062. pmid:17957246
  12. 12. Tito RY, Knights D, Metcalf J, Obregon-Tito AJ, Cleeland L, Najar F et al. (2012) Insights from characterizing extinct human gut microbiomes. PLoS One 7: e51146. pmid:23251439
  13. 13. Bos KI, Herbig A, Sahl J, Waglechner N, Fourment M, Forrest SA et al. (2016) Eighteenth century Yersinia pestis genomes reveal the long-term persistence of an historical plague focus. Elife 5: pii e12994.
  14. 14. Bos KI, Schuenemann VJ, Golding GB, Burbano HA, Waglechner N, Coombes BK et al. (2011) A draft genome of Yersinia pestis from victims of the Black Death. Nature 478: 506–510. pmid:21993626
  15. 15. Wagner DM, Klunk J, Harbeck M, Devault A, Waglechner N, Sahl JW et al. (2014) Yersinia pestis and the plague of Justinian 541–543 AD: a genomic analysis. Lancet Infect Dis 14: 319–326. pmid:24480148
  16. 16. Devault AM, Golding GB, Waglechner N, Enk JM, Kuch M, Tien JH et al. (2014) Second-pandemic strain of Vibrio cholerae from the Philadelphia cholera outbreak of 1849. N Engl J Med 370: 334–340. pmid:24401020
  17. 17. Keller A, Graefen A, Ball M, Matzas M, Boisguerin V, Maixner F et al. (2012) New insights into the Tyrolean Iceman's origin and phenotype as inferred by whole-genome sequencing. Nat Commun 3: 698. pmid:22426219
  18. 18. Maixner F, Thomma A, Cipollini G, Widder S, Rattei T, Zink A. (2014) Metagenomic analysis reveals presence of Treponema denticola in a tissue biopsy of the Iceman. PLoS One 9: e99994. pmid:24941044
  19. 19. Ng TF, Chen LF, Zhou Y, Shapiro B, Stiller M, Heintzman PD et al. (2014) Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch. Proc Natl Acad Sci U S A 111: 16842–16847. pmid:25349412
  20. 20. Allentoft ME, Collins M, Harker D, Haile J, Oskam CL, Hale ML et al. (2012) The half-life of DNA in bone: measuring decay kinetics in 158 dated fossils. Proc R Soc B 279: 4724–4733. pmid:23055061
  21. 21. Wadsworth C, Buckley M (2014) Proteome degradation in fossils: investigating the longevity of protein survival in ancient bone. Rapid Commun Mass Spectrom 28: 605–615. pmid:24519823
  22. 22. Buckley M, Wadsworth C (2014) Proteome degradation in ancient bone; what ancient proteins can tell us. Palaeogeography, Palaeoclimatology, Palaeoecology 416: 69–79.
  23. 23. Lowenstein JM (1993) In Organic Geochemistry: Immunospecificity of fossil proteins. 817–827 (Springer).
  24. 24. Hollemeyer K, Altmeyer W, Heinzle E, Pitra C (2008) Species identification of Oetzi’s clothing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on peptide pattern similarities of hair digests. Rapid Commun Mass Spectrom 22: 2751–2767. pmid:18720427
  25. 25. Huynh HT, Verneau J, Levasseur A, Drancourt M, Aboudharam G (2015) Bacteria and archaea paleomicrobiology of the dental calculus: a review. Mol Oral Microbiol pmid:26194817
  26. 26. Gurley LR, Valdez JG, Spall WD, Smith BF, Gillette DD (1991) Proteins in the fossil bone of the dinosaur, Seismosaurus. J Protein Chem 10: 75–90. pmid:2054066
  27. 27. Signoli M, Chausserie-Laprée J, Dutour O (1995) Etude anthropologique d’un charnier de peste de 1720–1721 à Martigues. Préhistoire et anthropologie méditerranéennes. 4, 173–189.
  28. 28. Tsujino N, Sakurai T (2009) Orexin/hypocretin: a neuropeptide at the interface of sleep, energy homeostasis, and reward system. Pharmacol Rev 61: 162–176. pmid:19549926
  29. 29. Eckhardt A, Jágr M, Pataridis S, Mikšík I (2014) Proteomic Analysis of Human Tooth Pulp: Proteomics of Human Tooth. J Endodontics 40: 1961–1966.
  30. 30. Bianucci R, Rahalison L, Ferroglio E, Massa ER, Signoli M (2007) A rapid diagnostic test for plague detects Yersinia pestis F1 antigen in ancient human remains. C R Biol 330: 747–754. pmid:17905394
  31. 31. Drancourt M, Signoli M, Dang LV, Bizot B, Roux V, Tzortzis S et al. (2007) Yersinia pestis Orientalis in remains of ancient plague patients. Emerg Infect Dis 13: 332–333. pmid:17479906
  32. 32. Aboudharam G, La VD, Davoust B, Drancourt M, Raoult D (2005) Molecular detection of Bartonella spp. in the dental pulp of stray cats buried for a year. Microbial Pathogenesis 38: 47–51. pmid:15652295
  33. 33. Nguyen-Hieu T, Aboudharam G, Signoli M, Rigeade C, Drancourt M, Raoult D (2010) Evidence of a louse-borne outbreak involving typhus in Douai, 1710–1712 during the war of Spanish succession. PLoS One 5: 1710–1712.
  34. 34. Papagrigorakis MJ, Yapijakis C, Synodinos PN, Baziotopoulou-Valavani E (2006) DNA examination of ancient dental pulp incriminates typhoid fever as a probable cause of the Plague of Athens. Int J Infect Dis 10: 206–214. pmid:16412683
  35. 35. Corthals A, Koller A, Martin DW, Rieger R, Chen EI, Bernaski M et al. (2012) Detecting the Immune System Response of a 500 Year-Old Inca Mummy. PLoS One 7: e41244. pmid:22848450
  36. 36. Maixner F, Overath T, Linke D, Janko M, Guerriero G, van den Berg BH et al. (2013) Paleoproteomic study of the Iceman’s brain tissue. Cell Mol Life Sci 70: 3709–3722. pmid:23739949
  37. 37. Malou N, Tran TN, Nappez C, Signoli M, Le Forestier C, Castex D et al. (2012) Immuno-PCR—a new tool for paleomicrobiology: The plague paradigm. PLoS One 7: e31744. pmid:22347507
  38. 38. Dorm M (2012) Nancy, Meurthe-et-Moselle. Ilôt Berger-Levrault. Du village Saint-Dizier au cimetière des Trois Maisons. Rapport Final d’Opération de fouille préventive 2010. INRAP Grand Est Nord, Service Régional de l’Archéologie de Lorraine, vol 4.
  39. 39. Cappellini E, Jensen LJ, Szklarczyk D, Ginolhac A, da Fonseca RA, Stafford TW et al. (2012) Proteomic analysis of a pleistocene mammoth femur reveals more than one hundred ancient bone proteins. J Proteome Res 11: 917–926. pmid:22103443