A. No effects observed in the wound-healing assay, following siRNA transfections. Confluent monolayers of Mero-14 cells transfected with 40 nM of siCtrl, or siMSLN-1, respectively. Two different experiments were carried out, each performed in triplicate. B. Trans-well cell invasion assay on Mero-14 cells transfected with 40 nM of the siCtrl (top), or siMSLN-1 (bottom). Pictures were taken using a fluorescence microscope at 10X magnification and are reported as negative of the originals to enhance the contrast between the background and the DAPI-stained cells. The bar chart shows the average of invasive cells (error bars represent SEM of two independent experiments, each done in triplicate, *P = 0.0044).
Citation: Melaiu O, Stebbing J, Lombardo Y, Bracci E, Uehara N, Bonotti A, et al. (2017) Correction: MSLN gene silencing has an anti-malignant effect on cell lines overexpressing mesothelin deriving from malignant pleural mesothelioma. PLoS ONE 12(6): e0180317. https://doi.org/10.1371/journal.pone.0180317
Published: June 22, 2017
Copyright: © 2017 Melaiu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.