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Association between tooth loss and cognitive decline: A 13-year longitudinal study of Chinese older adults

  • Juan Li,

    Affiliations Nursing School of Second Military Medical University, Shanghai, China, Duke University School of Nursing, Durham, United States of America

  • Hanzhang Xu,

    Affiliations Duke University School of Nursing, Durham, United States of America, Duke Global Health Institute, Durham, United States of America

  • Wei Pan,

    Affiliation Duke University School of Nursing, Durham, United States of America

  • Bei Wu

    Affiliation New York University Rory Meyers College of Nursing, New York, United States of America

Association between tooth loss and cognitive decline: A 13-year longitudinal study of Chinese older adults

  • Juan Li, 
  • Hanzhang Xu, 
  • Wei Pan, 
  • Bei Wu



To examine the association between the number of teeth remaining and cognitive decline among Chinese older adults over a 13-year period.


A large national longitudinal survey of Chinese older adults


The Chinese Longitudinal Healthy Longevity Survey (CLHLS) (1998–2011).


A total of 8,153 eligible participants aged 60+ interviewed in up to six waves.


Cognitive function and teeth number were measured at each interview. Cognitive function was measured by the Mini-Mental Status Examination (MMSE). Number of natural teeth was self-reported. Individuals with severe cognitive impairment were excluded. Covariates included demographic characteristics, adult socioeconomic status characteristics, childhood socioeconomic status, health conditions, and health behaviors. Linear mixed models were applied in the analysis.


The mean teeth number at baseline was 17.5(SD = 0.1), and the mean of baseline cognitive function was 27.3(SD = 0.0). Cognitive function declined over time (β = -0.19, P < .001) after controlling covariates. But, regardless of time, more teeth were associated with better cognitive function (β = 0.01, P < .001). The interaction of teeth number and time was significant (β = 0.01, P < .001), suggesting that the participants who had more teeth showed a slower pace of cognitive decline over time than those with fewer teeth after controlling for other covariates.


This study showed that tooth loss was associated with cognitive decline among Chinese older adults. Further studies are needed to examine the linkages between cognitive decline and oral health status using clinical examination data.


Cognitive impairment is common among older adults. The prevalence of mild cognitive impairment (MCI) in China was 20.1% among older adults aged 60 and over in 2010 [1].

MCI constitutes an intermediate stage between normal aging and dementia, and is considered a pre-dementia syndrome [2], as the probability of MCI in adults progressing to dementia is higher than adults without MCI. The annual progression rate from MCI to dementia at 5.9% is much higher than normal cognition to dementia, of 0.6%, based on the findings from U.S studies [3], The burden of cognitive impairment in China will continue to increase in the future, as almost 290 million people are over 55[4,5].

It is important to identify risk factors of MCI in order to delay the onset and decline of cognitive impairment. Limited education, depression, chronic diseases, lack of physical activity, and poor dietary habits have all been identified as possible risk factors in previous studies[68]. There is increasing evidence that suggests poor oral health may be a potential risk factor for cognitive decline[68]. In recent years, growing number of studies conducted in developed countries have focused on the relationship between oral health and dementia or cognitive decline[615]. Some studies showed a negative relationship between good oral health and cognitive decline or dementia[10,13].

There is little data from longitudinal research, and studies in the field have been inconclusive in their findings. A 5-year prospective cohort study of 11,140 participants aged 55–88 with type 2 diabetes showed that those with 0 teeth or 1–21 teeth had higher risk of dementia and cognitive decline than those with 22 and more teeth [10]. However, other studies did not find the significant relationship between number of teeth and dementia or cognitive impairment [11,12,16]. An 8-year prospective cohort study of 5468 older adults showed that men and women who were edentulous or had 1–15 teeth or 16–25 teeth at baseline did not have a significantly higher risk of dementia compared to those with 26–32 natural teeth [11]. These studies used the baseline teeth number to predict the later dementia onset or cognitive decline, and the teeth number was typically coded as a categorical variable that the categorization varied by studies. Very limited studies were conducted to examine the relationship between number of teeth and the trajectory of cognitive function or decline over time. Further studies are needed to use time-specific data of teeth number and cognition and treat them as continuous variables to capture how the decrease in teeth number is related to cognitive decline. In addition, most previous studies used shorter length of the study period, while our study used 6 waves of data over a period of 13 years.

Further, no studies have examined the association between tooth loss and cognitive impairment among Chinese populations using longitudinal data. Some factors influencing oral health and cognitive function in China could be very different from those of developed countries. These factors consist of knowledge and awareness of oral health problems, oral diseases and cognitive decline, medical insurance policies, access to dental care, nutrition intake, and exposure to risk factors [17,18]. Given the rapid increase in the older adults population and related increase in the prevalence of cognitive impairment in China, it is important to use empirical evidence to examine the association between tooth loss and cognitive decline.

Our study also controlled some important covariates, such as childhood socioeconomic status (SES) in the analysis. Tooth loss is an accumulative process across life span [19]. Childhood SES has an impact on health outcomes in later life, and lower childhood SES is associated specifically with poor adult oral health [20,21]. Additionally, lower childhood SES has been found to be associated with cognitive decline in old age [22,23]. A test of tooth loss’ relationship with trajectory of cognitive function while controlling for childhood SES is particularly interesting. The purpose of this study is to explore the association between tooth number and cognitive decline among Chinese older adults over a 13-year period controlling for a number of covariates.


Study population

This study used Chinese Longitudinal Healthy Longevity Survey (CLHLS) data collected in the period between 1998 and 2011 with the following data collection points: 1998, 2000, 2002, 2005, 2008, and 2011. The goal of CLHLS was to better understand the determinants of healthy longevity of Chinese elderly. Participants in the CLHLS were selected from counties and cities in 22 of China’s 31 provinces. The population in these survey areas constituted approximately 85% of the total population in China. The first and second waves of CLHLS, conducted in 1998 and 2000, respectively, were limited to persons aged 80 and above. A relatively younger sample—older adults aged 60–79—were added to the CLHLS survey since 2002 and had been included in all subsequent waves. Surviving participants were re-interviewed at each follow-up wave of the CLHLS, and new participants were enrolled to make up for attrition due to loss to follow-up and death. In the CLHLS, a weight of age–sex–urban/rural residence in the sample with the distribution of the total population in the sampled 22 provinces was employed to reflect the unique sample design. Detailed information about the CLHLS sampling design and data quality were reported elsewhere [24].

Our study used a combined sample based on the five cohorts of data (1998, 2000, 2002, 2005, and 2008) to get a sufficiently large enough sample size and provide robust estimates for the 13-year study period (1998–2011) [2527]. The first interview of each participant was at baseline, and interview of participants ranged from two to six separate waves of the data. In order to measure the association between the change in teeth number and cognitive decline, we excluded the following participants: 1) 20,643, had only one wave data due to death, loss to follow-up, or system missing; 2) 6,141, completely edentulous at initial wave; 3) 6,948, had logistic error of changes of tooth number; and 4) 262, had severe cognitive impairment with MMSE score less than ten [28]. This study included 8,153 eligible participants aged 60 and above which had at least two wave data.

The CLHLS study was approved by research ethics committees of Duke University and Peking University (IRB00001052-13074). All participants provided written informed consent. No experimental interventions were performed. An exempted Institutional Review Board (IRB) protocol was approved by Duke University (Pro00062871).

Outcome variable

Cognitive function was measured by the Chinese version of Mini-Mental State Examination (MMSE) at each wave, which pertained to orientation, registration, attention, calculation, recall, and language [29]. MMSE score ranges from 0 to 30, with lower score indicating poorer cognition. There are three possible answers for each question: correct = 1, wrong = 0, and unable to answer. We coded responses of “unable to answer” as incorrect answers based on previous literature [23].

Independent variable

Self-reported number of teeth was collected at each wave using the following question: “How many natural teeth do you still have?” The teeth number ranges from 0 to 32 including the third molar.


The main covariates were demographic characteristics, adult SES, childhood SES, health conditions and health behaviors. Demographic variables include age, gender (male = 1, female = 0), ethnicity (Han = 1, Non-Han = 0), and marital status (married = 1, others = 0). Adult SES was a continuous variable ranging from 0 to 3 with 1 point each if the respondent answered yes to the questions:(1) having 1+years education;(2) primary lifetime occupation was professional;(3) urban resident [30]. Childhood SES was a continuous variable ranging from 0 to 5 with 1 point each if the respondent answered yes to the questions: (1) obtained adequate medical services at childhood; (2) went to bed without hunger at childhood; (3) both parents were alive when the participant was age 14;(4) the participant’s father’s occupation was professional/administration; (5) place of birth was urban [22]. Health conditions included self-rated health status, self-reported chronic disease (hypertension, diabetes, heart disease, stroke or cerebrovascular disease, lung disease), and activities of daily life (ADL). Self-rated health status had two options (good = 1, poor = 0). Individual ADL was measured by the Katz index scale that consisted of six items: bathing, dressing, indoor transferring, toileting, eating, and continence [31]. The ADL disability was the number of activities that individuals needed help performing, ranging from 0 to 6. Health behaviors included current smoking (coded as 1) and current drinking (coded as 1). A time variable was also derived to indicate the time of each interview since entering into CLHLS study (time = 0,2,3,4,5,6,7,8,9,10,11,13) in relation to the CLHLS baseline and follow-up. A cohort variable was generated to indicate which cohort the participants were from.

Age, gender, ethnicity, and education, primary lifetime occupation, childhood SES were measured at baseline. All other covariates (marital status, adult SES, self-rated health, chronic diseases, ADL disabilities, current smoking and drinking levels) were measured at baseline and at each follow-up interview.

Statistical analysis

Descriptive statistics were used to summarize baseline socio-demographic status, medical conditions, and health behaviors. A series of linear mixed models were used to estimate the association between change of teeth number and cognitive decline (time = 0,2,3,4,5,6,7,8,9,10,11,13) over time. The procedure of PROC MIXED in SAS Version 9.3 (SAS Institute Inc., Cary, NC) was used to estimate the parameters of the linear mixed models. Weights were applied to reflect the unique sampling design of the CLHLS. The missing data accounted for less than 5% of the dataset, but were not completely at random as evidenced by using Missing Completely at Random test. Therefore, a multiple imputation approach was used to reduce the potential for inferential bias and minimize the loss of subjects due to missing items [32]. All continuous variables among independent variable and covariates were centered at their grand mean values, except time and the dummy variables before entering the linear mixed models, so the estimates were more interpretable [33].

Baseline characteristics of subjects were reported as mean and standard deviation (SD) for continuous variables and numbers and proportion for categorical variables. All analyses were conducted using the programs of SAS 9.3 (SAS Institute, Inc., Cary, NC, USA).P< .01 was considered to be statistically significant because of the large sample size [34].


Descriptive statistics

Table 1 shows the baseline socio-demographic characteristics of the study participants (N = 8153). The “unweighted” outcome in Table 1 meant the outcome from the original sample. The “weighted” outcome in Table 1 meant the outcome after using the weight to adjust the age-sex-urban/rural residence sampling strategy in order to represent the total population in the sampled 22 provinces. For the weighted outcomes, the mean age of all subjects was 74.4, and the percentage of male participants was 46.4%. About 54.8% participants were married, 50.3% participants had at least 1 year of education, and 64.6% subjects lived in rural areas. Ten percent of subjects had a professional or administrative occupation. More than half of the participants reported good health status (54.5%). The mean ADL disability was 0.1. The percentages of current smoker and drinker were 25.3% and 23.0%, respectively. The mean of baseline teeth number was 17.5(SD = 0.1), and the mean of baseline cognitive function was 27.3(SD = 0.0).

Table 1. Description of characteristics of the participants at baseline (N = 8153).

Linear mixed model analyses

Table 2 summarizes the results of linear mixed models of multiple factors on cognitive function. Results from Model I showed that cognitive function declined with time over the follow-up period (β = -0.12, P < .001), which meant that when time increased about 1 year, the MMSE score decreased by 0.12 points. But, regardless of time, more teeth were associated with better cognitive function (β = 0.09, P < .001), which meant that for patients who had one more tooth than the other patients, their MMSE score increased by 0.09 points. Model II showed that after adding socio-demographic variables in the model, tooth number and time were still significant. Subjects who were female, older, unmarried, with lower adult SES and lower childhood SES were more likely to have poorer cognitive function. Model III showed results controlling for health status, ADL, current smoking, current drinking, and cohort effects. Participants who reported poorer self-rated health, suffered from stroke or cerebrovascular disease, or had ADL disability were more likely to have poorer cognitive function. After adding the interaction between tooth number and time, Model IV showed that cognitive function declined over time (β = -0.19, P < .001) after controlling for covariates, which meant that when time increased about 1 year, the MMSE score decreased by 0.19 points. Fewer teeth were associated with poorer cognitive function regardless of time (β = 0.01, P < .001), which meant that patients who lose one tooth, the MMSE score decreased by 0.01 points. The interaction of tooth number and time was significant (β = 0.01, P < .001), which meant that for patients who had one more tooth than the other patients, their MMSE score increased by 0.01 points about every year. It further suggests that the participants who had more teeth showed a slower pace of cognitive decline over time than those with fewer teeth after controlling for other covariates.

Table 2. The results from linear mixed models of the covariates and tooth number on cognitive function: 13-year follow-up data.


This study is the first longitudinal study to examine the association between teeth number and cognitive decline in Chinese older adults. Teeth number and cognitive function were measured at each time point and treated as continuous variables, in order to explore the trajectory of cognitive decline and the association with tooth loss. Overall, cognitive function declined over the study period after controlling for covariates. Tooth loss was prospectively associated with cognitive decline in Chinese older adults. The higher number of tooth loss, the faster cognitive function declined. This finding added new evidence to the relationship between oral health and cognitive function.

The result of the present study is consistent with previous longitudinal studies. Case-control studies showed that history of tooth loss was a significant risk factor for poor cognitive function [6,35]. In one study, each tooth loss per decade since the baseline dental examination increased the likelihood of a low MMSE score in 597 men aged 28–70 at baseline and followed–up to 32 years in the USA [7]. Other longitudinal studies also showed the association between tooth loss and the increased risk of cognitive decline [10,13,36]. These results demonstrated that tooth loss is a risk factor for cognitive decline.

However, one longitudinal study didn’t find the association between tooth loss and cognitive decline [14]. Stewart and colleagues reported that fewer teeth was not significantly associated with cognitive decline according to quartile of teeth number at baseline (OR = 0.88, 95%CI 0.77–1.00) in a sample of 1053 Black and White men and women aged 70–79 in the USA. This inconsistency may be due to difference in race/ethnicity, age range, follow-up period, definition of cognitive decline and the statistical methods used. The follow-up period to evaluate cognitive decline in the study was short, only 2 years. The study used the quartile of teeth number at baseline and applied a logistic regression model. In contrast, our study had relatively long follow-up period (13 years), used both teeth number and cognitive function as continuous and time-varying variables, which were up to 6 waves, and applied linear mixed models. So our study may provide a more robust outcome.

There are several possible physiological mechanisms to explain the pathways between tooth loss and cognitive decline. One possibility is periodontitis, which is one of the main causes of tooth loss [3739]. Inflammatory factors derived from the body’s response to periodontal infection may disseminate to brain through the systemic circulation and exacerbate inflammatory process and vascular pathologies [4044]. The second possible pathway is poor nutrition due to tooth loss, including intake of insufficient recommended levels of foods, nutrients and B vitamins, which may be linked to cognitive decline [4547]. A third possible pathway may be the decreased masticatory function as a result of tooth loss. Several clinical and animal studies had suggested that mastication was effective in sending sensory information to the brain and in maintaining learning and memory functions of the hippocampus [4850]. Reduced masticatory function is associated with the hippocampal morphological impairments and the hippocampus-dependent spatial memory deficits, and cognitive decline especially in elderly [51].

Oral diseases are very common in China, particularly among older adults. The Third National Oral Health Epidemiological Survey in China conducted in 30 provinces had reported that 98.4% of older adults aged 65 to 74 years had cavities, and 54% of older adults thought their teeth needed to be treated [52]. But the needs for dental treatment among Chinese elderly people have been unmet. Among older adults, 98.1% of the cavities had not been treated. There are several reasons for the high percentage of oral health problems that are untreated in older adult population in China. They include a general lack of knowledge of the importance of oral health, public healthcare policy, primary care providers who are not trained in oral healthcare, and education of the general population. The percentages of older adults having a dental visit in the past 12 months were 19%. About 30% of older adults had never visited a dentist during their lifetime, and only about 7% of older adults received preventive oral services during the past one year in 2005 [53]. Only 26% of the 65-74-year-old adults brushed their teeth twice a day, and 27% of the older adults used toothpaste with fluoride [52,53]. Oral health knowledge education programs are urgently needed among older adults population. Another additional problem with good oral health care in China, is that medical insurance policies do not cover much if any dental care service. In 2003, the Urban Residents Basic Medical Insurance Scheme (URBMI) and the New Rural Cooperative Medical Scheme(NRCMS) were created by National Health and Family Planning Commission of the People’s Republic of China, but the problems of low compensation rate to healthcare providers, no reimbursement for outpatient care (including dental care) and the absence of preventative healthcare for the population insured under URBMI and NRCMS programmes, has resulted in minimal improvement of oral care [54]. About 83% of older adults must pay for the whole cost of dental care by themselves, and 26.3% of older adults are not able to afford dental treatment in 2005 [53]. There is also a shortage of trained dentists in China, 8.64 dentists per 100,000 people, which is lower than the average level in the world, 26.32 dentists per 100,000 people [55]. Establishing a preventive oral health care system, implementing community-based oral health education, oral examination and intervention, encouraging more attendance to dental schools to help bring up the dentist number, would begin to address the oral healthcare problem in China. For health care policy reform in China, it would be important to expand medical insurance coverage to include dental care.

The strengths of the present study were relatively large sample size and long follow-up period. Meanwhile, teeth number and cognitive function were measured for every wave and treated as continuous and time-varying variable, so that we could observe the trajectory of cognitive decline and the significant association between tooth loss and cognitive decline.

One limitation of the study was Mini-Mental State Examination (MMSE). MMSE is the most widely used instrument for the screening of cognitive impairment worldwide, but it is age, race, and education biased, and has a ceiling effect, which may not be sensitive for MCI [5658]. Another limitation was self-reported teeth number. However, self-reported teeth number has been widely used as a measure of oral health in epidemiological surveys exploring the relationship between oral health and cognitive function [10,11,59,60]. Previous studies conducted in the USA demonstrated that self-reported numbers of remaining teeth was strongly correlated with clinical records(r = 0.74–1.0) [61,62]. To avoid recall bias of the participants, especially those with severe cognitive impairment, the observations’ data whose MMSE score was 0–9 indicating severe cognitive impairment in the data cleaning process, was excluded. If there was a logistic error in teeth number change, after review, the cases were excluded. Thus, the teeth number information in the study should be reliable. A total of 32 teeth (including the third molar) was used as the tooth number because extracting the third molar is not common in China. Another limitation is the reporting of general oral health in our study. There was no detailed information about periodontal disease, remaining teeth’s position and quality, denture size and type, and inflammatory biomarkers.


This is the first longitudinal study reporting the association between teeth number and cognitive decline in Chinese elderly. The results from this study showed that more teeth were associated with better cognitive function, and participants who had fewer teeth tended to show a quicker pace of cognitive decline in Chinese older adults. The findings may have clinical implications on improvement of oral health and cognitive function. Further studies are needed to examine the linkages between cognitive decline and oral health status from more detailed clinical examination data.


We thank Dr. Huashuai Chen for his interpretation about the CLHLS database, Dr. Qing Yang for her advice on data analysis.

Author Contributions

  1. Conceptualization: JL BW.
  2. Data curation: JL HZ.
  3. Formal analysis: JL HZ.
  4. Methodology: JL HZ WP BW.
  5. Project administration: BW.
  6. Resources: BW.
  7. Software: JL HZ.
  8. Supervision: BW.
  9. Validation: HZ WP.
  10. Writing – original draft: JL.
  11. Writing – review & editing: HZ BW.


  1. 1. Ding D, Zhao Q, Guo Q, Meng H, Wang B, Luo J, et al. Prevalence of mild cognitive impairment in an urban community in China: A cross-sectional analysis of the Shanghai Aging Study. Alzheimer’s Dement. Elsevier Ltd; 2015;11: 300–309.e2.
  2. 2. Langa KM, Levine DA. The diagnosis and management of mild cognitive impairment: a clinical review. Jama. 2014;312: 2551–61. pmid:25514304
  3. 3. Gao S, Unverzagt FW, Hall KS, Lane KA, Murrell JR, Hake AM, et al. Mild cognitive impairment, incidence, progression, and reversion: findings from a community-based cohort of elderly African Americans. Am J Geriatr Psychiatry. 2014;22: 670–81. pmid:23831172
  4. 4. Jia J, Zhou A, Wei C, Jia X, Wang F, Li F, et al. The prevalence of mild cognitive impairment and its etiological subtypes in elderly Chinese. Alzheimer’s Dement. Elsevier Ltd; 2014;10: 439–447.
  5. 5. The world factbook-China. In: Central Intelligence Agency [Internet]. [cited 3 Jul 2016].
  6. 6. Gatz M, Mortimer JA, Fratiglioni L, Johansson B, Berg S, Reynolds CA, et al. Potentially modifiable risk factors for dementia in identical twins. Alzheimers Dement. 2006;2: 110–7. pmid:19595867
  7. 7. Kaye EK, Valencia A, Baba N, Spiro A, Dietrich T, Garcia RI. Tooth loss and periodontal disease predict poor cognitive function in older men. J Am Geriatr Soc. 2010;58: 713–8. pmid:20398152
  8. 8. Tsakos G, Watt R, Rouxel PL, de Oliveira C, Demakakos P. Tooth Loss Associated with Physical and Cognitive Decline in Older Adults. J Am Geriatr Soc. 2004;324: 1417–216.
  9. 9. Arrivé E, Letenneur L, Matharan F, Laporte C, Helmer C, Barberger-Gateau P, et al. Oral health condition of French elderly and risk of dementia: A longitudinal cohort study. Community Dent Oral Epidemiol. 2012;40: 230–238. pmid:22059867
  10. 10. Batty G-D, Li Q, Huxley R, Zoungas S, Taylor B-A, Neal B, et al. Oral disease in relation to future risk of dementia and cognitive decline: prospective cohort study based on the Action in Diabetes and Vascular Disease: Preterax and Diamicron Modified-Release Controlled Evaluation (ADVANCE) trial. Eur Psychiatry. 2013;28: 49–52. pmid:21964484
  11. 11. Paganini-Hill A, White SC, Atchison KA. Dentition, dental health habits, and dementia: the Leisure World Cohort Study. J Am Geriatr Soc. 2012;60: 1556–1563. pmid:22860988
  12. 12. Shimazaki Y, Soh I, Saito T, Yamashita Y, Koga T, Miyazaki H, et al. Influence of Dentition Status on Physical Disability, Mental Impairment, and Mortality in Institutionalized Elderly People. J Dent Res. 2001;80: 340–345. pmid:11269726
  13. 13. Stein PS, Desrosiers M, Donegan SJ, Yepes JF, Kryscio RJ. Tooth loss, dementia and neuropathology in the Nun study. J Am Dent Assoc. American Dental Association; 2007;138: 1314–1322-1382.
  14. 14. Stewart R, Weyant RJ, Garcia ME, Harris T, Launer LJ, Satterfield S, et al. Adverse oral health and cognitive decline: the health, aging and body composition study. J Am Geriatr Soc. 2013;61: 177–84. pmid:23405916
  15. 15. Stewart R, Stenman U, Hakeberg M, Hägglin C, Gustafson D, Skoog I. Associations between oral health and risk of dementia in a 37-year follow-up study: the prospective population study of women in Gothenburg. J Am Geriatr Soc. 2015;63: 100–5. pmid:25597561
  16. 16. Stewart R, Stenman U, Hakeberg M, Catharina H. Associations Between Oral Health and Risk of Dementia in a 37-Year Follow-Up Study : The Prospective Population Study of Women in Gothenburg. 2015; 100–105.
  17. 17. Stewart R, Sabbah W, Tsakos G, D’Aiuto F, Watt RG. Oral health and cognitive function in the Third National Health and Nutrition Examination Survey (NHANES III). Psychosom Med. 2008;70: 936–41. pmid:18842752
  18. 18. Wu B, Plassman BL, Liang J, Wei L. Cognitive function and dental care utilization among community-dwelling older adults. Am J Public Health. 2007;97: 2216–21. pmid:17971546
  19. 19. Thorstensson H, Johansson B. Why do some people lose teeth across their lifespan whereas others retain a functional dentition into very old age? Gerodontology. 2010;27: 19–25. pmid:19545321
  20. 20. Thomson WM, Poulton R, Milne BJ, Caspi A, Broughton JR, Ayers KMS. Socioeconomic inequalities in oral health in childhood and adulthood in a birth cohort. Community Dent Oral Epidemiol. 2004;32: 345–53. pmid:15341619
  21. 21. Starr JM, Hall R. Predictors and correlates of edentulism in healthy older people. Curr Opin Clin Nutr Metab Care. 2010;13: 19–23. pmid:19898233
  22. 22. Han W-J, Shibusawa T. Trajectory of physical health, cognitive status, and psychological well-being among Chinese elderly. Arch Gerontol Geriatr. Elsevier Ireland Ltd; 2015;60: 168–177.
  23. 23. Zhang Z, Gu D, Hayward MD. Childhood nutritional deprivation and cognitive impairment among older Chinese people. Soc Sci Med. Elsevier Ltd; 2010;71: 941–9.
  24. 24. Gu D. General Data Quality Assessment for the 2005 CLHLS wave. Cent Study Aging Hum Dev Med Cent, Duke Univ. 2008;2002: 1–20.
  25. 25. Wen M, Gu D. The effects of childhood, adult, and community socioeconomic conditions on health and mortality among older adults in China. Demography. 2011;48: 153–181. pmid:21394657
  26. 26. Yi Z, Gu D. Chapter 4 Reliability of Age Reporting among the Chinese Oldest-old in the CLHLS datasets. Dordr Springer. 2008; 61–78.
  27. 27. Zimmer Z, Korinek K. Shifting coresidence near the end of life: Comparing decedents and survivors of a follow-up study in China. Demography. 2010;47: 537–554. pmid:20879676
  28. 28. Yin Z-X, Shi X-M, Kraus VB, Fitzgerald SM, Qian H-Z, Xu J-W, et al. High normal plasma triglycerides are associated with preserved cognitive function in Chinese oldest-old. Age Ageing. 2012;41: 600–6. pmid:22447910
  29. 29. Katzman R, Zhang MY, Ouang-Ya-Qu , Wang ZY, Liu WT, Yu E, et al. A Chinese version of the Mini-Mental State Examination; impact of illiteracy in a Shanghai dementia survey. J Clin Epidemiol. 1988;41: 971–8. pmid:3193141
  30. 30. Luo Y, Waite LJ. The impact of childhood and adult SES on physical, mental, and cognitive well-being in later life. J Gerontol B Psychol Sci Soc Sci. 2005;60: S93–S101. pmid:15746030
  31. 31. Katz S., Branch L. G., Branson M. H., Papsidero J. A., Beck J. C., & Greer DS. Active life expectance. N Engl J Med. 1983;309: 1218–1224. pmid:6633571
  32. 32. Allison PD. Missing Data. In: SAGE Publications Inc. 2001.
  33. 33. Singer JD, Willett JB. Applied longitudinal data analysis: Modeling change and event occurrence. New York: Oxford University Press; 2003.
  34. 34. Nuzzo R. Scientific method: Statistical errors. Nature. 2014;506: 150–152. pmid:24522584
  35. 35. Kondo K, Niino M, Shido K. A case-control study of Alzheimer’s disease in Japan—significance of life-styles. Dementia. 1994;5: 314–26. pmid:7866485
  36. 36. Stein PS, Kryscio RJ, Desrosiers M, Donegan SJ, Gibbs MB. Tooth Loss, Apolipoprotein E, and Decline in Delayed Word Recall. J Dent Res. 2010;89: 473–477. pmid:20139337
  37. 37. Al-Shammari KF, Al-Khabbaz AK, Al-Ansari JM, Neiva R, Wang H-L. Risk indicators for tooth loss due to periodontal disease. J Periodontol. 2005;76: 1910–8. pmid:16274310
  38. 38. Martinez-Canut P. Predictors of tooth loss due to periodontal disease in patients following long-term periodontal maintenance. J Clin Periodontol. 2015;42: 1115–1125. pmid:26498672
  39. 39. Chambrone L, Chambrone D, Lima LA, Chambrone LA. Predictors of tooth loss during long-term periodontal maintenance: A systematic review of observational studies. J Clin Periodontol. 2010;37: 675–684. pmid:20528960
  40. 40. Kamer AR, Craig RG, Dasanayake AP, Brys M, Glodzik-Sobanska L, de Leon MJ. Inflammation and Alzheimer’s disease: Possible role of periodontal diseases. Alzheimer’s Dement. 2008;4: 242–250.
  41. 41. Watts A, Crimmins EM, Gatz M. Inflammation as a potential mediator for the association between periodontal disease and Alzheimer’s disease. Neuropsychiatr Dis Treat. 2008;4: 865–76. pmid:19183779
  42. 42. Bretz W a., Weyant RJ, Corby PM, Ren D, Weissfeld L, Kritchevsky SB, et al. Systemic inflammatory markers, periodontal diseases, and periodontal infections in an elderly population. J Am Geriatr Soc. 2005;53: 1532–1537. pmid:16137283
  43. 43. Pink C, Kocher T, Meisel P, Dörr M, Markus MRP, Jablonowski L, et al. Longitudinal effects of systemic inflammation markers on periodontitis. J Clin Periodontol. 2015;
  44. 44. Noack B, Genco RJ, Trevisan M, Grossi S, Zambon JJ, De Nardin E. Periodontal infections contribute to elevated systemic C-reactive protein level. J Periodontol. 2001;72: 1221–7. pmid:11577954
  45. 45. Tucker KL, Qiao N, Scott T, Rosenberg I, Spiro A. High homocysteine and low B vitamins predict cognitive decline in aging men: The Veterans Affairs Normative Aging Study. Am J Clin Nutr. 2005;82: 627–635. pmid:16155277
  46. 46. Krall E, Hayes C, Garcia R. How dentition status and masticatory function affect nutrient intake. J Am Dent Assoc. 1998;129: 1261–9. pmid:9766107
  47. 47. Kim J-M, Stewart R, Prince M, Kim S-W, Yang S-J, Shin I-S, et al. Dental health, nutritional status and recent-onset dementia in a Korean community population. Int J Geriatr Psychiatry. 2007;22: 850–5. pmid:17266172
  48. 48. De Cicco V, Barresi M, Fantozzi MPT, Cataldo E, Parisi V, Manzoni D. Oral implant-prostheses: New teeth for a brighter brain. PLoS One. 2016;11: 1–21.
  49. 49. Ono Y, Yamamoto T, Kubo K, Onozuka M. Occlusion and brain function: mastication as a prevention of cognitive dysfunction. J Oral Rehabil. 2010;37: 624–640. pmid:20236235
  50. 50. Miyake S, Wada-Takahashi S, Honda H, Takahashi S, Sasaguri K, Sato S, et al. Stress and chewing affect blood flow and oxygen levels in the rat brain. Arch Oral Biol. Elsevier Ltd; 2012;57: 1491–1497.
  51. 51. Chen H, Iinuma M, Onozuka M, Kubo K-Y. Chewing Maintains Hippocampus-Dependent Cognitive Function. Int J Med Sci. 2015;12: 502–509. pmid:26078711
  52. 52. Prevention CC for DC and. China third oral epidemiological survey outcome [Internet]. 2007 [cited 8 Dec 2015].
  53. 53. Qi X. The third National Oral Health Survey. 1st ed. Beijing: People’s Medical Publishing House; 2008.
  54. 54. Meng Q, Tang S. Universal Coverage of Health Care in China: Challenges and Opportunities. Heal Syst Financ path to Univers Cover. 2010;
  55. 55. Gao B, Li G, Wang Y, Guo J, Hao Y, Ding C. The investigationon dentist structure in some provinces and a city of China. J Pract Stomatol. 2011;27: 279–282.
  56. 56. Spencer RJ, Wendell CR, Giggey PP, Katzel LI, Lefkowitz DM, Siegel EL, et al. Psychometric Limitations of the Mini-Mental State Examination Among Nondemented Older Adults: An Evaluation of Neurocognitive and Magnetic Resonance Imaging Correlates. Exp Aging Res. 2013;39: 382–397. pmid:23875837
  57. 57. Trzepacz T, Hochstetler H, Wang S, Walker B, Saykin J, Alzheimer’s Initiative D. Relationship between the Montreal Cognitive Assessment and Mini-mental State Examination for assessment of mild cognitive impairment in older adults. BMC Geriatr. BMC Geriatrics; 2015;15: 107. pmid:26346644
  58. 58. Scazufca M, Almeida OP, Vallada HP, Tasse W a, Menezes PR. Limitations of the Mini-Mental State Examination for screening dementia in a community with low socioeconomic status: results from the Sao Paulo Ageing & Health Study. Eur Arch Psychiatry Clin Neurosci. 2009;259: 8–15. pmid:18560791
  59. 59. Wu B, Plassman BL, Liang J, Remle RC, Bai L, Crout RJ. Differences in Self-Reported Oral Health Among Community-Dwelling Black, Hispanic, and White Elders. J Aging Health. 2011;23: 267–288. pmid:20858912
  60. 60. Luo J, Wu B, Zhao Q, Guo Q, Meng H, Yu L, et al. Association between Tooth Loss and Cognitive Function among 3063 Chinese Older Adults : A Community-Based Study. PLoS One. 2015;10: 1–11.
  61. 61. Douglass CW, Berlin J, Tennstedt S. The validity of self-reported oral health status in the elderly. J Public Health Dent. 1991;51: 220–2. pmid:1941773
  62. 62. Pitiphat W, Garcia RI, Douglass CW, Joshipura KJ. Validation of self-reported oral health measures. J Public Health Dent. 2002;62: 122–8. pmid:11989207