(A) Real-time RT-PCR analysis of Pdrg1 expression using the 18s gene as reference in the stable silenced clones (3–44 and 4–18) and the negative control clone (CN-10) prepared in H35 cells. The results shown are the mean ± SEM of four independent experiments carried out in triplicate. (B) Growth curves for H35 wild type cells (♦), the CN-10 (■) and 4–18 (●) clones; the figure shows the mean ± SEM of eight replicates of a representative independent experiment from the four carried out. (C) Pathway analysis of genes exhibiting expression changes ≥2-fold using Gene Ontology; only pathways with p<0.05 are indicated. (D) Real-time RT-PCR verification of expression changes (mean ± SEM; N = 4) in selected genes using the Rn18s gene as reference.
Citation: Pérez C, Pérez-Zúñiga FJ, Garrido F, Reytor E, Portillo F, Pajares MA (2016) Correction: The Oncogene PDRG1 Is an Interaction Target of Methionine Adenosyltransferases. PLoS ONE 11(9): e0163761. https://doi.org/10.1371/journal.pone.0163761
Published: September 22, 2016
Copyright: © 2016 Pérez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.