Variation in local environmental conditions can have pronounced effects on the population structure and dynamics of marine organisms. Previous studies on crown-of-thorns starfish, Acanthaster planci, have primarily focused on effects of water quality and nutrient availability on larval growth and survival, while the role of maternal nutrition on reproduction and larval development has been overlooked. To examine the effects of maternal nutrition on oocyte size and early larval development in A. planci, we pre-conditioned females for 60 days on alternative diets of preferred coral prey (Acropora abrotanoides) versus non-preferred coral prey (Porites rus) and compared resulting gametes and progeny to those produced by females that were starved over the same period. Females fed ad libitum with Acropora increased in weight, produced heavier gonads and produced larger oocytes compared to Porites-fed and starved females. Fed starfish (regardless of whether it was Acropora or Porites) produced bigger larvae with larger stomachs and had a higher frequency of normal larvae that reached the late bipinnaria / early brachiolaria stage compared to starved starfish. Females on Acropora diet also produced a higher proportion of larvae that progressed to more advanced stages faster compared to Porites-fed starfish, which progressed faster than starved starfish. These results suggest that maternal provisioning can have important consequences for the quality and quantity of progeny. Because food quality (coral community structure) and quantity (coral abundance) varies widely among reef locations and habitats, local variation in maternal nutrition of A. planci is likely to moderate reproductive success and may explain temporal and spatial fluctuations in abundance of this species.
Citation: Caballes CF, Pratchett MS, Kerr AM, Rivera-Posada JA (2016) The Role of Maternal Nutrition on Oocyte Size and Quality, with Respect to Early Larval Development in The Coral-Eating Starfish, Acanthaster planci. PLoS ONE 11(6): e0158007. https://doi.org/10.1371/journal.pone.0158007
Editor: Jason Glenn Knott, Michigan State University, UNITED STATES
Received: March 8, 2016; Accepted: June 8, 2016; Published: June 21, 2016
Copyright: © 2016 Caballes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are available in the Tropical Data Hub - James Cook University's Research Data Repository (DOI: http://dx.doi.org/10.4225/28/56E622E699A75).
Funding: This study was funded by the Australian Research Council Centre of Excellence for Coral Reef Studies PhD research allocation to CFC and annual research appropriation to MSP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Episodic population outbreaks of the crown-of-thorns starfish, Acanthaster planci, have resulted in widespread degradation of Indo-Pacific coral reefs . While the ultimate cause of outbreaks is still the subject of debate, most researchers agree that exploring the reproductive biology and early life history of A. planci is essential in understanding mechanisms that lead to outbreaks. The two most prominent hypotheses that seek to explain the cause(s) of outbreaks, the ‘terrestrial runoff hypothesis’ [2–5] and ‘predator removal hypothesis’ [6,7] are built upon variations in larval survival, growth, and development in response to starvation and predation. Populations of A. planci are predisposed to major fluctuations due to inherent properties of their life history such as high fecundity [8,9], high fertilization rates [10,11], and short generation times [12,13]. Small environmental and biological changes, therefore, could potentially lead to rapid increases in the abundance of A. planci .
Fluctuations in larval survival and development can have pronounced effects on recruitment rates and hence the dynamics of adult populations [5,15]. Increased larval nutrition has a positive effect on the condition of the larvae of A. planci, as it does on other marine organisms with planktotrophic larvae. . Past studies on the survival and development of larvae of A. planci have primarily been centered on the direct effects of nutrient concentrations and exogenous food availability in the water column [3,16–18]. However, the role of maternal nutrition on reproduction and larval development of A. planci has generally been overlooked. The few studies that have explored effects of maternal condition in A. planci examined the effects of food availability on gametogenesis. Cheney  reported that total deprivation of coral food for one month by caging resulted in shrinking of gonads, deterioration of pyloric caeca, and decrease in total diameter. Conversely, gravid A. planci collected from Okinawa and starved for 90 days showed no change in the size and condition of gonads even though pyloric caeca were reduced to thin ribbons .
The quantity and quality of food available to adult starfish can have flow-on effects on overall reproductive capacity . Coral composition and abundance are often variable in nature [22,23] and local conditions can influence the nutritional status of corallivores, like A. planci. It is well established that A. planci have distinct feeding preferences and Acropora (along with Montipora) are among the most preferred genera, consistently eaten in preference to other locally abundant corals [1,24,25]. Although Porites are much less preferred, they are not totally avoided and are often consumed when more preferred species have been depleted . Starved individuals have also been observed towards the end of outbreak events when live coral prey becomes scarce .
Natural variation in food availability and nutrient assimilation can influence gamete production in echinoderms . Starvation has been found to result in the failure of gonads to achieve normal size increments . In asteroids, most nutrients used in gametogenesis are processed and stored in the pyloric caecum and delivered directly to the gonads . Echinoderms found in favorable habitats with abundant supply of preferred food items often respond by increasing body weight, gonad size, and pyloric caeca index . For example, Scheibling and Lawrence  found that starfish (Echinaster sp.) found on seawalls with abundant supply of oysters, sponges, ascidians, and bryozoans were almost three times heavier than individuals from less favorable sites. Moreover, green sea urchins (Strongylocentrotus droebachiensis) from shallower depths, where preferred macroalgal food was more abundant, had larger gonads compared to females from food-limited deeper sites . The weight of the pyloric caeca for Pisaster ochraceus from wave-exposed sites, where food items are more abundant, was significantly higher compared to starfish from wave-protected sites . Laboratory studies also confirmed that adults on rich food diets during active gametogenesis have higher body weight, gonad size, and pyloric caeca index . This was demonstrated by experimental manipulation of diet in the New Zealand starfish, Sclarasterias mollis, where body weight, and gonad and pyloric caeca index increased significantly compared to starved starfish [34,35]. The lipid content of fed starfish was also higher than in starved groups, although protein and carbohydrate contents in gonads did not vary significantly between feeding treatments .
Maternal nutrition can alter resource allocation mechanisms and affect nutrient investment in oocytes [36,37]. Oocyte size, fecundity, and oocyte quality can vary with the nutritional history of adults [38–41]. Because oocyte size is a function of maternal investment, selection on oocyte size is a function of maternal fitness . Echinoderms found at sites with abundant food supply and in high food laboratory treatments mostly produced higher numbers of large, high quality oocytes . Female starfish (Leptasterias epichlora) from high food availability, exposed sites produced higher numbers of larger oocytes with higher protein content compared to those from sheltered sites . Starfish collected from less favorable sheltered sites subsequently placed under high food ration treatments in the laboratory had more oocytes with larger diameter compared to starfish in low food treatments .
For free-spawning invertebrates, the entire maternal contribution to subsequent generations is provided in the oocyte . Following fertilization and gastrulation, the digestive tract differentiates (early bipinnaria stage) and larvae enter the facultative feeding period (FFP); at this stage, larvae are able to feed but do not necessarily require food because maternal provisions from the oocyte are still available . During the initial larval stages, maternal provisioning can have important consequences for starvation resistance, mortality risk from predation, and developmental rates [46,47], ultimately leading to reduced planktonic duration and increased settlement success. In the absence of exogenous food, larvae from large oocytes (high maternal investment) have significant buffering from longer periods of starvation during the FFP . Predation rates during the vulnerable stage of larval development are also moderated by rapid larval development .
In this study, we examined the role of experimental variation in maternal nutrition (comparing between individuals that were starved, fed on preferred corals and fed on generally non-preferred coral prey) on the larval growth and early development prior to exogenous feeding by larvae. The effect of maternal nutrition on the following aspects of reproduction and larval development in A. planci are specifically addressed in this study: (1) adult female morphometrics before and after treatment; (2) gonad and pyloric caeca indexes; (3) oocyte size and shape; (4) fertilization rates; (5) early larval growth; (6) larval survival; and (7) larval development. Few studies have investigated the effect of the nutritional state of the adult A. planci on oocyte size, fertilization, larval growth and development. This has important implications on the survival of larvae when exogenous food supply is low or absent. Development and growth rates of the larvae of A. planci are predicted to be low in the absence of enhanced phytoplankton levels [3–5,18]. However, it is not known whether increased maternal investment allows larvae to withstand prolonged periods of starvation and proceed with normal development. Because food quality (coral community structure) and quantity (coral abundance) varies widely between adult populations of A. planci in coral reefs, any effect of maternal nutrition on larval quality and survivorship may influence the overall reproductive success of A. planci and help explain marked fluctuations in abundance.
Specimen Collection and Ethics Statement
All experiments were conducted at the University of Guam Marine Laboratory (UOGML) in accordance with regulations set out by the University of Guam and James Cook University. Crown-of-thorns starfish were collected on SCUBA from reefs at the southern end of Ague Point (13.565360°N, 144.819119°E) on the northwest coast of Guam and immediately transported to UOGML. No permits were required to collect A. planci. Sex was determined by examining contents drawn from gonads along the arm junction using a syringe with a large-bore biopsy needle . Male and female A. planci were maintained in separate flow-through tanks. Male A. planci were maintained on a mix of Acropora spp., Porites spp., and Pocillopora spp. corals as soon as they were introduced to holding tanks, while female A. planci were starved for 10 days prior to being assigned to one of three different feeding treatments (described below). Coral collections were done under a special license issued by the Guam Department of Agriculture–Division of Aquatic and Wildlife Resources to UOGML (in accordance with Section 63123 of Title 5, Guam Code Annotated). Acropora abrotanoides colonies were collected from Pago Bay and Porites rus colonies were collected from Western Shoals, Guam. Aside from being abundant in these collection sites , these species were selected because A. abrotanoides is among the most highly preferred prey coral species in Guam, whereas P. rus is one of the least preferred species . Columnar and tabular/plate growth forms of these corals were collected to make surface area measurements easier. Coral infauna (e.g., Trapezia crabs) were physically removed from all coral fragments so as not to deter feeding by A. planci .
Nine female A. planci with intact arms and approximately similar diameter (ca. 320 ± 7 mm) were placed in individual plastic bins with flow through ambient seawater (temperature = 29.07 ± 0.47°C; salinity = 32.97 ± 0.06 psu; pH = 8.30 ± 0.05). All females were nearing reproductive maturity based on microscopic examination of oocytes drawn from starfish using the biopsy procedure described in the previous section. Oogenesis in A. planci usually takes between two and three months, although some oocytes can complete oogenesis within a month . Starfish were assigned to one of three different feeding treatments (n = 3) for 60 days: i) Starved (no food), ii) Acropora (fed with Acropora abrotanoides), and iii) Porites (fed with Porites rus). Supply of coral food for fed treatments was replenished as soon as the piece of coral provided has been completely consumed. Since live coral was used in the diet of ad libitum fed starfish, the only way to reduce the amount of coral used was to minimize the sample size. Growth of each individual starfish was quantified based on changes in diameter (Δd) and weight (Δw) from day 0 to day 60. At the end of the feeding experiment (day 60) we also calculated the gonad index (GI) and the pyloric caeca index (PCI) for each individual. The average weight of gonads and pyloric caeca from three arms was multiplied with the total number of arms of each starfish to estimate the total gonad or pyloric caeca weight. GI and PCI were expressed as the ratio of gonad or pyloric caeca weight to the total weight of the starfish .
To relate differences in physiological and reproductive condition to food intake, we calculated the rate of feeding for each individual starfish by measuring the total surface area of coral consumed throughout the 60 day period. Consumed coral fragments were weighed dry and the surface area of each fragment was estimated following the foil-wrapping technique. Each piece of coral was tightly molded with heavy-duty aluminum foil to fit depressions and projections, following Marsh . The aluminum foil molds were flattened and digital photographs were taken using a ruler as scale. These pictures were analyzed by calculating the area (cm2) of the flattened molds using the image analysis software, Image J .
Spawning Induction and Oocyte Dimensions
To test the effect of feeding treatments on oocyte metrics, gonads were dissected from the nine females and ovaries were rinsed in 0.2-μm filtered seawater (FSW) to remove loose oocytes. Ovary lobes were treated in 10−5 M 1-methyladenine to induce ovulation. Released oocytes were transferred into containers with filtered seawater and wet mounted on glass slides for microscopic examination. Oocytes were photographed with a camera (Canon EOS 60D) mounted on a microscope (Leica DM300) with a calibrated ocular micrometer. Ayukai et al.  developed a criterion to estimate oocyte quality in A. planci based on morphometric characteristics. Oocytes that were relatively large (> 0.15 mm in diameter), spherical (round), and uniform in size and shape often achieved successful fertilization, embryogenesis, and gastrulation . Diameters (doocyte) of the long and short axes of 100 randomly selected mature oocytes (have undergone germinal vesicle breakdown) from each treatment were measured using Image J . Oocyte volume (voocyte) was calculated using the formula for an oblate spheroid: 4/3 × π × (long axis radius)2 × short axis radius. Oocyte sphericity is the ratio of the long and short axis diameter measurements.
Oocytes from each female were placed in separate 1 L beakers with FSW kept at 28°C. Approximately 200 oocytes from each female were transferred into triplicate 250-ml beakers using a glass pipette. Spermatozoa were collected from the testes of 5 males and checked for motility under a microscope. Approximately equal amounts of spermatozoa from each male were combined and counted using a haemocytometer. Oocytes were fertilized with spermatozoa diluted to achieve a spermatozoa-to-oocyte ratio of 100:1. After 10 minutes, an aliquot of eggs was subsampled from each replicate and further development halted with 7% formalin before examination. One hundred eggs were examined and eggs with raised fertilization envelopes were counted and percent fertilization was calculated.
Fertilized eggs from the nine females were separately reared in triplicates at 28°C and after 24 hours, 50 actively swimming gastrulae were siphoned into separate glass culture jars with 200 ml FSW. Each jar was equipped with a plastic stirring paddle attached to a 20-rpm synchronous motor. Water changes with fresh FSW were performed three times daily. Surviving larvae in each jar, regardless of developmental stage, were counted daily for eight days during the second water change. After four days, 10 normally developing larvae from each jar were placed in a relaxing agent (7% MgCl2) for 10 minutes and fixed in 10% formalin in FSW. Larvae were immediately photographed using a camera mounted on a microscope shortly after fixation and total length, width, and stomach area were measured using ImageJ (Fig 1).
Image analysis measurements of length, width, and stomach area of four-day old larvae.
After eight days, all surviving larvae were categorized into the following developmental stages: (1) early bipinnaria–preoral and anal lobes present, coelomic pouches below or close to mouth; (2) advanced bipinnaria–coelomic pouches above the mouth and almost touching, anterodorsal and posterolateral arms start to form; (3) late bipinnaria / early brachiolaria–anterior extension of fused coelomic sacs, anterodorsal and posterolateral arms longer, posterodorsal arms start to elongate, preoral arms start to form; and (–) abnormal (stunted, deformed) development [12,13,33,55]. The percentage of normally developing larvae after eight days was also calculated. No food was provided to the larvae during the entire duration of the experiment to control for variation in the food intake of individual larvae.
Mean daily consumption by each female was analyzed using one-way analysis of variance (ANOVA) to test for differences in total area of live coral consumed on exclusive diets of Acropora versus Porites. Change in diameter and weight (= post-treatment–pre-treatment), post-treatment gonad index, and post-treatment pyloric caeca index were compared between feeding treatments (3 levels, fixed) using one-way ANOVA followed by post hoc Tukey’s pairwise comparisons in SPSS 22.0 (IBM Corporation, NY, USA). Proportion of fertilized eggs were arcsine square-root transformed prior to nested ANOVA analysis with ‘Maternal Nutrition’ as fixed effects and ‘Female’ as a random factor nested under ‘Maternal Nutrition’. Normality and homogeneity of variance of data on oocyte dimensions, proportion of normal larvae, and proportion of larvae reaching late bipinnaria / early brachiolaria stage after eight days did not improve after transformations. A nested (hierarchical) permutational analysis of variance (PERMANOVA) with ‘Female’ (3 levels, random) nested within ‘Maternal Nutrition’ (3 levels, fixed) was run to analyze differences between and within feeding treatments. PERMANOVA is a non-parametric technique that may also be used in analyzing univariate data (Anderson et al. 2008). Analyses were conducted using the PERMANOVA+ add-on for PRIMER v.6 (Primer-E Ltd., Plymouth, UK), and used the Euclidean distance measure, Type III sums of squares, and 9999 permutations of the residuals under a reduced model to calculate the significance of the pseudo-F statistic. In cases where not enough unique permutations (< 100) were possible to determine permutational p-values (pperm), Monte-Carlo asymptotic p-values (pMC) were used instead . Pairwise comparisons between Maternal Nutrition treatments were analyzed using Benjamini-Hochberg corrected pMC-values . Data on the proportion of surviving larvae were arcsine square-root transformed prior to daily comparisons using nested ANOVA with a similar model described for fertilization data. Statistical comparison of larval length, width, and stomach area were made using a three-factor nested ANOVA with ‘Maternal Nutrition’ as a fixed effect (3 levels), ‘Female’ (3 levels, random) nested within ‘Maternal Nutrition’ and ‘Jar’ (3 levels, random) nested within ‘Female’ and ‘Maternal Nutrition’. A post hoc Tukey’s Test was used for pairwise comparisons of fixed factor means. Variation in the proportion of larvae under 4 larval development categories between feeding treatments was analyzed using G-test of independence followed by post hoc pairwise comparisons applying Benjamini-Hochberg correction for multiple comparisons .
Coral Consumption and Morphometrics
Female A. planci provided with ad libitum rations of coral food exhibited a significant difference in consumption rates depending on the coral species (F1,5 = 55.309, p = 0.002). Starfish provided with A. abrotanoides consumed an average of 157.64 ± 10.71 cm2 of coral tissue per day while P. rus was consumed at rate of 101.81 ± 7.37 cm2 per day (Table 1). There was no significant difference in Δd between treatments (F2,8 = 3.413, p = 0.102); however, the change in weight (Δw) between treatments was significantly different (F2,8 = 5.816, p = 0.039) and post hoc pairwise comparisons show that weight gain in Acropora-fed females was significantly higher than in starved females (Table 1). Post-treatment gonad index (GI) was also significantly different between treatments (F2,8 = 7.530, p = 0.023), with values from Acropora-fed A. planci significantly higher than for starved starfish. Mean Δw and GI was not significantly different between Acropora- and Porites-fed starfish, nor between Porites-fed and starved females. Maternal treatments also had a significant effect on pyloric caeca index (PCI) values (F2,8 = 12.846, p = 0.007) and pairwise comparisons between treatments indicate that coral-fed starfish (regardless of whether they were maintained on Acropora or Porites) had higher pyloric caeca index values compared to starved A. planci. There was no significant difference in PCI between starfish in coral food treatments.
Oocyte Dimensions and Fertilization
Significant variation in oocyte diameter (pseudo-F2,891 = 11.463, pperm = 0.009) and calculated oocyte volume (pseudo-F2,891 = 15.316, pperm = 0.007) was noted among starfish in each of the three feeding treatments. The fixed effects accounted for 31% and 33.5% of variation in oocyte diameter and oocyte volume, respectively, while around 50% of variation was due to differences at the replicate level. Pairwise comparisons (Fig 2) showed that the diameter and volume of oocytes from Acropora-fed females (doocyte = 0.25 ± 0.02 mm; Voocyte = 8.27×10−3 ± 1.95×10−3 mm3) was significantly higher than oocytes from Porites-fed (doocyte = 0.23 ± 0.02 mm; Voocyte = 6.54×10−3 ± 1.77×10−3 mm3) and starved females (doocyte = 0.22 ± 0.04 mm; Voocyte = 5.52×10−3 ± 2.63×10−3 mm3). Oocyte diameter in this study was relatively higher compared to previously reported oocyte sizes of A. planci and other coral reef asteroids with planktotrophic mode of development (Table 2). Maternal nutrition also had a significant treatment effect on oocyte sphericity (pseudo-F2,891 = 9.675, pperm = 0.012), which accounted for 33.7% of variation while 47.1% of variation was due to differences at the replicate level. Oocytes from fed females (Acropora = 0.97 ± 0.02; Porites = 0.95 ± 0.03) were predominantly more spherical (ratio of long and short axis ≈ 1) than oocytes from starved females (0.90 ± 0.07), which were mostly ellipsoidal in shape. Oocyte diameter (pseudo-F2,891 = 9.759, pperm < 0.001), volume (pseudo-F2,891 = 8.625, pperm < 0.001), and sphericity (pseudo-F2,891 = 17.709, pperm < 0.001) also differed significantly among females within maternal nutrition treatments, but only accounted for less than 20% of the total variation in each parameter. Overall, the size and shape of oocytes was less uniform in starved starfish compared to oocytes from fed starfish.
Plots show median (dashed line), 25th and 75th percentile range in the grey box, 5th and 95th percentile range as error bars, and outliers as solid circles for oocyte (A) maximum diameter, (B) volume, and (C) sphericity index (n = 100). Different letters are significantly different based on post hoc pairwise comparisons.
Fertilization rates of eggs from individual females were high (88%-100%) across all treatment levels (Fig 3). Maternal nutrition did not have a significant effect on fertilization rates (F2,18) = 2.318, p = 0.180), despite significantly different oocytes sizes. There was also no significant variation in fertilization rates of eggs from individual female starfish within treatments (F2,18) = 2.477, p = 0.063).
Larval Growth, Survival and Development
After four days, surviving larvae were in the bipinnaria stage. Maternal nutrition had a significant effect on larval morphometrics at this stage (Fig 4). Larvae from females fed with Acropora (0.75 ± 0.12 mm) and Porites corals (0.72 ± 0.15 mm) were significantly longer than larvae from starved females (0.53 ± 0.14 mm) (Table 3: F2,243 = 20.351, p = 0.002; Fig 4A). Maternal nutrition also had a significant effect on the width of larvae at this stage (Table 3: F2,243 = 23.321, p = 0.001, Fig 4B). Larvae from fed females (Acropora: 0.52 ± 0.09 mm; Porites: 0.50 ± 0.11 mm) were significantly wider than larvae from starved females (0.37 ± 0.10 mm). A similar pattern was also observed in terms of stomach area (Table 3: F2,243 = 23.321, p = 0.001, Fig 4C), where larvae from Acropora- (5.28×10−3 ± 1.49×10−3 mm2) and Porites-fed (4.94×10−3 ± 1.64×10−3 mm2) females had larger stomachs than larvae from starved females (4.10×10−3 ± 1.68×10−3 mm2). It is interesting to consider that 4 days may not have been sufficient time to discern larval growth trajectories between the two fed treatments.
Image analysis measurements of (A) length, (B) width, and (C) stomach area (n = 10). Error bars are + 1 SD and different letters are significantly different based on Tukey’s post hoc test.
Maternal nutrition had no significant effect on daily survival rates (Fig 5), which remained above 60% across all levels after eight days of rearing. The highest mortality across all treatments was recorded four days after fertilization; starved females, in particular, decreased by an average of 8% at day four. Conversely, maternal nutrition had a significant effect on the proportion of larvae that developed normally (pseudo-F2,18 = 8.192, pperm = 0.011; Fig 6A). Among the surviving larvae after eight days, there was a higher proportion of normally developing larvae in the Acropora- (96 ± 2%) and Porites-fed (94 ± 3%) treatments compared to starved (68 ± 15%) treatments. This pattern was even more apparent when the proportion of larvae that reached the late bipinnaria / early brachiolaria stage was compared (pseudo-F2,18 = 177.720, pperm = 0.004; Fig 6B). The proportion that reached the late bipinnaria / early brachiolaria stage was ten times higher in the Acropora treatment (50 ± 6%) and nine times higher in the Porites treatment (44 ± 5%) compared to the starved treatment (5 ± 2%). Overall, the proportion of larvae that progressed to a new developmental stage was significantly different between treatments (G = 339.555, df = 6, p < 0.001; Fig 7). All pairwise comparisons were significant at Benjamini-Hochberg corrected alpha levels (Fig 7). There was a higher proportion of abnormal (31%) and early bipinnaria (43%) larvae in the starved treatment compared to the fed treatments. Larvae under the Acropora treatment were in relatively advanced stages with 32% at advanced bipinnaria and 50% at late bipinnaria / early brachiolaria stage. All normal larval stages were represented in the Porites treatment consisting of 22% at early bipinnaria, 29% at advanced bipinnaria, and 44% at late bipinnaria / early brachiolaria stage.
Data points are mean values ± 1SD of pooled proportions of surviving larvae from all females and rearing jars under each maternal nutrition treatment (n = 9).
Proportion of (A) normal larvae and (B) late bipinnaria / early brachiolaria larvae at day eight. Error bars represent + 1SD and n = 9 for each maternal treatment. Different letters are significantly different based on Tukey’s post hoc tests.
Proportion of larvae under 4 development categories: (1) early bipinnaria, (2) advanced bipinnaria, (3) late bipinnaria / early brachiolaria, and (–) abnormal larvae. Arrows and p-values represent post hoc G-test pairwise comparisons with Benjamini-Hochberg-corrected significance levels.
Average daily coral consumption of A. planci in this study falls within feeding rates observed in the field during summer months on the Great Barrier Reef . Consistent with field observations [24,25], consumption rates on A. abrotanoides was significantly higher compared to feeding rates on P. rus. One possible explanation for marked feeding preferences among corallivorous organisms (given that the order of preferences is not always consistent with energetic value of the different corals, e.g., Keesing ) is that preferred corals are those upon which feeding is most efficient, e.g. Cole and Pratchett . Accordingly, we found that the consumption rates of A. planci on A. abrotanoides (based on estimated area of live coral consumed each day) were 50% higher compared to similar sized starfish feeding on P. rus. This is because A. planci can digest the tissues of Acropora much more efficiently than tissues of Porites . Moreover, Acropora corals tend to have much deeper tissue layers than Porites, owing to their perforate skeleton . Given that Acropora corals have higher energetic content and greater tissue depth compared to Porites, and can also feed at greater rates on Acropora, it is expected that A. planci on Acropora would be in much better nutritional condition.
Despite differences in diet and food intake, no differences were apparent in the physical appearance of female A. planci that were starved for 60 days versus individuals maintained on exclusive diets of A. abrotanoides or P. rus. This reflects the considerable resilience of starfish to shortages in prey . However, differences in prey intake did appear to have a significant effect on the physiological condition of crown-of-thorns starfish, with flow-on effects for individual reproductive capacity. Most notably, the starfish that were fed (even if on sub-optimal coral prey, P. rus) tended to increase in weight over the course of the study, whereas starved individuals consistently lost weight. Fed individuals also had heavier gonads and pyloric caeca compared to starved individuals, even when standardized for overall body weight, as shown for other echinoderms . Xu and Barker  suggest that the pyloric caeca act as nutrient reservoirs to support reproductive and maintenance activities under conditions of nutritional stress.
Increases in gonad weight of well-fed echinoderms can be attributed to increased oocyte size and/or higher maternal fecundity . Similarly, the starfish, L. epichlora, had higher fecundity and produced bigger oocytes at sites with increased food availability . Bertram and Strathmann  found that gonad volume increased as a function of oocyte size in S. droebachiensis. The weight of A.planci gonads also partly reflects increased fecundity, whereby Conand  estimated that A.planci consistently produce 90,190 oocytes per gram of ovary. Our study clearly shows that individual starfish feeding on A. abrotanoides had proportionally larger gonads, and produced larger oocytes (diameter and volume) compared to Porites-fed and starved females. Oocytes from A. planci that were fed with A. abrotanoides were also more uniform and more spherical in shape compared to oocytes from starved females, which is generally reflective of higher oocyte quality, as well as leading to increased rates of fertilization and larval development .
It is also noteworthy that average oocyte diameter measurements in this study were relatively bigger than previously reported measurements for A. planci (Table 2). Since the mean oocyte diameter of starved treatments (0.22 ± 0.04 mm) in this study was still marginally higher than measurements from other localities, this variation may largely be from the source population and only partially due to the experimental manipulation of diet. More importantly, the biochemical and energetic composition of oocytes warrant further investigation because these measurements show that oocytes of A. planci are relatively bigger compared to oocytes from echinoderms that are obligate planktotrophs . Yolk-rich planktotrophic larvae of the Antarctic starfish, Porania antarctica have been found to differentiate to brachiolaria stage and increase in length even in the absence particulate food . This may help explain extreme population fluctuations in A. planci compared to other asteroids with similar planktonic life histories.
For A. planci, fertilization rates were not significantly different among treatments and were consistently high across all females regardless of differences in nutritional conditions, oocyte size and shape. Similarly, no significant variation in fertilization success was observed when the sea urchin, S. droebachiensis, was fed with artificial diets containing different levels of dietary protein and additives . However, Levitan  has shown that for the sea urchin Strongylocentrotus franciscanus, fertilization rates were higher on larger eggs, but only under sperm-limiting conditions. Variation in oocyte shape may also result in constrained or arrested development. For example, experiments in which echinoderm oocytes were artificially deformed resulted in abnormal cleavage patterns . Conversely, Podolsky and Strathmann  suggested that varying oocyte shape could provide a mechanism to increase oocyte-spermatozoa collisions without increasing oocyte volume. Our results warrant further investigation into whether any oocyte size and shape has an effect on fertilization rates at lower spermatozoa concentrations and whether the effect of environmental conditions could swamp the influence of gamete traits. High spermatozoa concentrations were used in this study to evaluate whether low maternal investment reduces fertilization rates. Spawning in turbulent water conditions, lack of synchrony, and low proximity to other spawning individuals could potentially limit spermatozoa concentrations and oocyte traits may be under intense selection to increase fertilization rates in the field.
Females that were fed (regardless of whether they were fed with A. abrotanoides or P. rus) produced larger larvae with larger stomachs compared to starved females. Maternally derived energetic lipids, particularly triglycerides, fuel larval development in planktotrophic starfish . High feeding rates by A. planci on a lipid-rich food source, such as corals [77,78], may allow excess resources for gametogenesis and provide increased maternal provisioning of lipids as a buffer against unfavorable nutritional conditions during the planktonic larval stage . For instance, differences in oocyte triglyceride levels were still observable at the bipinnaria stage for the planktotrophic starfish, Meridiastra mortenseni, indicating flow-on effects for larval fitness . The same major energetic lipid class (i.e. triglycerides) sequestered by feeding echinoderm larvae to support early juveniles is also provided by the female parent to fuel early development . Hence, additional energetic reserves allow larvae to withstand prolonged periods of starvation  and also may be used to produce larger larvae with morphologies that improve feeding effectiveness . These early larval stage metrics are useful indicators since larger larvae usually progress much faster to advanced stages and have higher survival later in development even at unfavorable food conditions for larvae . Although exogenous food supply may still be necessary to complete metamorphosis, faster growth reduces planktonic larval duration and exposure to larval predators .
Overall, survival rates were high across all treatments (>60%). Abnormal larvae continue swimming in the water column and remain alive for extended periods, but do not develop further . The proportion of stunted or deformed larvae from starved females was significantly higher and most larvae under this treatment remained at the early bipinarria stage after eight days. Even at starved larval conditions, as is the case in this study, a large proportion of larvae from fed females progressed from early bipinnaria to late bipinnaria / early brachiolaria stage. This implies that any surplus of maternally derived energetic lipids may support development even at conditions when exogenous food resources are limited. In assessing the role of exogenous food availability on larvae from these different conditions of maternal diet, the patchy nature of planktonic food resources must be considered . At low phytoplankton levels, developing larvae of A. planci may also exploit other sources of food to supplement endogenous nutrient reserves . When planktonic food is abundant, larvae of S. droebachiensis adults from nutritionally rich habitats have been shown to metamorphose sooner than larvae from adults collected from habitats with low food availability .
In summary, A. planci given almost limitless access to A. abrotanoides, which is among the most preferred coral prey, increased in weight and had heavier gonads compared to starved females. Acropora-fed females also produced larger oocytes compared to Porites-fed and starved females. Fed starfish produced bigger larvae with larger stomachs and had a higher frequency of normally developing larvae. Females on Acropora diet also produced larvae that progressed to more advanced stages faster compared to Porites-fed starfish, which progressed faster than starved starfish. These results show that the influence of maternal diet on oocyte characteristics was carried over to early larval stages, affecting both larval size and development. Variability at these earlier stages of development has been known to persist even after metamorphosis and impact juvenile quality [43,82]. This has significant implications for the reproductive capacity of female starfish living in reef habitats with varying coral community structure and abundance. Importantly, the local abundance of preferred prey (e.g., Acropora) could have an important impact on the overall reproductive capacity and resistance to larval starvation in the absence of phytoplankton blooms. Dense aggregations of brachiolaria-stage larvae of A. planci were detected in areas where phytoplankton concentrations were low , suggesting that apart from larval nutrition provided by nutrient-rich waters, other factors may play a role in larval survival and development. Future studies need to carefully consider the nutritional condition of females from which oocytes are collected in looking at the effect of larval nutrition on development. Future work should also measure the biochemical composition of oocytes from females under different nutritional states and also contrast the magnitude of the effects of maternal nutrition (endogenous) and larval nutrition (exogenous) on larval vitality and morphometry to see if these differences disappear through compensation or persist throughout development.
We thank the ARC Centre of Excellence for Coral Reef Studies for providing support and the University of Guam Marine Laboratory, through Prof. Laurie Raymundo, for providing workspace and assistance in specimen collection. We are grateful to Prof. Maria Byrne and Dr. Makoto Kitamura for providing technical advice. We also thank Joseph Cummings, Jason Miller, Travis Reynolds, Jordan Gault, Samuel Nietze, Mareen Moller, Andres Reyes and Maia Raymundo for laboratory and field assistance.
Conceived and designed the experiments: CFC MSP. Performed the experiments: CFC. Analyzed the data: CFC MSP AMK. Contributed reagents/materials/analysis tools: MSP JRP AMK. Wrote the paper: CFC MSP AMK JRP.
- 1. Pratchett MS, Caballes CF, Rivera-Posada JA, Sweatman HPA. Limits to understanding and managing outbreaks of crown-of-thorns starfish (Acanthaster spp.). Oceanogr Mar Biol An Annu Rev. 2014;52: 133–200.
- 2. Birkeland C. Terrestrial runoff as a cause of outbreaks of Acanthaster planci (Echinodermata: Asteroidea). Mar Biol. 1982;69: 175–185.
- 3. Lucas JS. Quantitative studies of feeding and nutrition during larval development of the coral reef asteroid Acanthaster planci (L.). J Exp Mar Bio Ecol. 1982;65: 173–193.
- 4. Brodie JE, Fabricius KE, De’ath G, Okaji K. Are increased nutrient inputs responsible for more outbreaks of crown-of-thorns starfish? An appraisal of the evidence. Mar Pollut Bull. 2005;51: 266–78. pmid:15757727
- 5. Fabricius KE, Okaji K, De’ath G. Three lines of evidence to link outbreaks of the crown-of-thorns seastar Acanthaster planci to the release of larval food limitation. Coral Reefs. 2010;29: 593–605.
- 6. Endean R. Acanthaster planci infestations of reefs of the Great Barrier Reef. In: Taylor DL, editor. Proceedings of the 3rd International Coral Reef Symposium. Miami, USA: Rosential School of Marine and Atmospheric Science—University of Miami; 1977. pp. 185–191.
- 7. Sweatman HPA. No-take reserves protect coral reefs from predatory starfish. Curr Biol. 2008;18: R598–R599. pmid:18644332
- 8. Conand C. Distribution, reproductive cycle and morphometric relationships of Acanthaster planci (Echinodermata: Asteroidea) in New Caledonia, western tropical Pacific. Proceedings of the 5th International Echinoderm Conference. Galway; 1985. pp. 499–506.
- 9. Kettle BT, Lucas JS. Biometric relationships between organ indices, fecundity, oxygen consumption and body size in Acanthaster planci (L.) (Echinodermata; Asteroidea). Bull Mar Sci. 1987;41: 541–551.
- 10. Babcock RC, Mundy CN, Whitehead D. Sperm diffusion models and in situ confirmation of long-distance fertilization in the free-spawning asteroid Acanthaster planci. Biol Bull. 1994;186: 17–28.
- 11. Benzie JAH, Dixon P. The effects of sperm concentration, sperm:egg ratio, and gamete age on fertilization success in crown-of-thorns starfish (Acanthaster planci) in the laboratory. Biol Bull. 1994;186: 139–152.
- 12. Yamaguchi M. Early life histories of coral reef asteroids, with special reference to Acanthaster planci (L.). In: Jones OA, Endean R, editors. Biology and Geology of Coral Reefs. New York: Academic Press, Inc.; 1973. pp. 369–387.
- 13. Caballes CF, Pratchett MS. Reproductive biology and early life history of the crown-of-thorns starfish. In: Whitmore E, editor. Echinoderms: Ecology, Habitats and Reproductive Biology. New Yor: Nova Science Publishers, Inc.; 2014. pp. 101–146. https://doi.org/10.13140/RG.2.1.3719.5129
- 14. Uthicke S, Schaffelke B, Byrne M. A boom–bust phylum? Ecological and evolutionary consequences of density variations in echinoderms. Ecol Monogr. 2009;79: 3–24.
- 15. Balch T, Scheibling RE. Larval supply, settlement and recruitment in echinoderms. In: Lawrence JM, Jangoux M, editors. Echinoderms Studies, Vol 6. Rotterdam: A.A. Balkema; 2001. pp. 1–83.
- 16. Olson RR. In situ culturing as a test of the larval starvation hypothesis for the crown-of-thorns starfish, Acanthaster planci. Limnol Oceanogr. 1987;32: 895–904.
- 17. Okaji K, Ayukai T, Lucas JS. Selective feeding by larvae of the crown-of-thorns starfish, Acanthaster planci (L.). Coral Reefs. 1997;16: 47–50.
- 18. Wolfe K, Graba-Landry A, Dworjanyn SA, Byrne M. Larval starvation to satiation: Influence of nutrient regime on the success of Acanthaster planci. PLoS One. 2015;10: 1–18.
- 19. Cheney DP. Spawning and aggregation of Acanthaster planci in Micronesia. Proceedings of the Second International Coral Reef Symposium. Brisbane: Great Barrier Reef Committee; 1974. pp. 591–594.
- 20. Okaji K. Delayed spawning activity in dispersed individuals of Acanthaster planci in Okinawa. In: Yanagisawa T, Yasumasu I, Oguro C, Motokawa N, Suzuki T, editors. Biology of Echinodermata. Atami, Japan: Balkema, Rotterdam; 1991. pp. 291–295.
- 21. George SB. Echinoderm egg and larval quality as a function of adult nutritional state. Oceanol Acta. 1996;19: 297–308.
- 22. Veron JEN. Natural Heritage Attribute: Hard Corals. In: Lucas P, Webb T, Valentine P, Marsh H, editors. The Outstanding Universal Value of the Great Barrier Reef World Heritage Area. Townsville, Australia: Great Barrier Reef Marine Park Authority; 1997. pp. 152–154.
- 23. Burdick D, Brown V, Asher J, Caballes CF, Gawel M, Goldman L, et al. Status of the coral reef ecosystems of Guam. Guam: Bureau of Statistics and Plans—Guam Coastal Management Program; 2008. https://doi.org/10.13140/RG.2.1.2183.8960
- 24. De’ath G, Moran PJ. Factors affecting the behaviour of crown-of-thorns starfish (Acanthaster planci L.) on the Great Barrier Reef:: 1: Patterns of activity. J Exp Mar Bio Ecol. 1998;220: 83–106.
- 25. Pratchett MS, Schenk TJ, Baine M, Syms C, Baird AH. Selective coral mortality associated with outbreaks of Acanthaster planci L. in Bootless Bay, Papua New Guinea. Mar Environ Res. Elsevier Ltd; 2009;67: 230–236. pmid:19327821
- 26. Kayal M, Vercelloni J, Lison de Loma T, Bosserelle P, Chancerelle Y, Geoffroy S, et al. Predator crown-of-thorns starfish (Acanthaster planci) outbreak, mass mortality of corals, and cascading effects on reef fish and benthic communities. PLoS One. 2012;7: e47363. pmid:23056635
- 27. Zann LP. Crown-of-thorns starfish, Acanthaster planci, in the proposed Ofu Marine Park, American Samoa. Honolulu, Hawaii: Cooperative National Park Resources Studies Unit, University of Hawaii; 1992.
- 28. Mercier A, Hamel J. Endogenous and Exogenous Control of Gametogenesis and Spawning in Echinoderms. Sims DW, editor. Advances in Marine Biology. London: Academic Press, Inc.; 2009. https://doi.org/10.1016/S0065-2881(09)55001-8
- 29. Nimitz MA. Histochemical changes in gonadal nutrient reserves correlated with nutrition in the sea stars, Pisaster ochraceus and Patiria miniata. Biol Bull. 1976;151: 357. pmid:974164
- 30. Shirai H, Walker CW. Chemical control of asexual and sexual reproduction in echinoderms. In: Laufer H, Downer RGH, editors. Invertebrate Endocrinology, Vol 2 Endocrinology of Selected Invertebrate Types. New York: Alan R. Liss, Inc.; 1988. pp. 453–476.
- 31. Scheibling RE, Lawrence JM. Differences in reproductive strategies of morphs of the genus Echinaster (Echinodermata: Asteroidea) from the Eastern Gulf of Mexico. Mar Biol. 1982;70: 51–62.
- 32. Bertram DF, Strathmann RR. Effects of maternal and larval nutrition on growth and form of planktotrophic larve. Ecology. 1998;79: 315–327.
- 33. George SB. Egg quality, larval growth and phenotypic plasticity in a forcipulate seastar. J Exp Mar Bio Ecol. 1999;237: 203–224.
- 34. Xu RA, Barker MF. Effect of diet on steroid levels and reproduction in the starfish, Sclerasterias mollis. Comp Biochem Physiol Part A Physiol. 1990;96: 33–39.
- 35. Xu RA, Barker MF. Laboratory experiments on the effects of diet on the gonad and pyloric caeca indices and biochemical composition of tissues of the New Zealand starfish Sclerasterias mollis (Hutton) (Echinodermata: Asteroidea). J Exp Mar Bio Ecol. 1990;136: 23–45.
- 36. Russell MP. Resource allocation plasticity in sea urchins: rapid, diet induced, phenotypic changes in the green sea urchin, Strongylocentrotus droebachiensis (Müller). J Exp Mar Bio Ecol. 1998;220: 1–14.
- 37. Lawrence JM, Plank LR, Lawrence AL. The effect of feeding frequency on consumption of food, absorption efficiency, and gonad production in the sea urchin Lytechinus variegatus. Comp Biochem Physiol Part A Mol Integr Physiol. 2003;134: 69–75.
- 38. George SB. Population and seasonal differences in egg quality of Arbacia lixula (Echinodermata: Echinoidea). Invertebr Reprod Dev. 1990;17: 111–121.
- 39. George SB, Cellario C, Fenaux L. Population differences in egg quality of Arbacia lixula (Echinodermata: Echinoidea): proximate composition of eggs and larval development. J Exp Mar Bio Ecol. 1990;141: 107–118.
- 40. George SB, Lawrence JM, Fenaux L. The effect of food ration on the quality of eggs of Luidia clathrata (Say) (Echinodermata: Asteroidea). Invertebr Reprod Dev. 1991;20: 237–242.
- 41. de Jong-Westman M, Qian P-Y, March BE, Carefoot TH. Artificial diets in sea urchin culture: Effects of dietary protein level and other additives on egg quality, larval morphometrics, and larval survival in the green sea urchin, Strongylocentrotus droebachiensis. Can J Zool. 1995;73: 2080–2090.
- 42. Levitan DR. Effects of gamete traits on fertilization in the sea and evolution of sexual dimorphism. Nature. 1996;382: 153–155.
- 43. George SB. Population differences in maternal size and offspring quality for Leptasterias epichlora (Brandt) (Echinodermata: Asteroidea). J Exp Mar Bio Ecol. 1994;175: 121–131.
- 44. Jaeckle WB. Variation in size, energy content, and biochemical composition of invertebrate eggs: Correlates to the mode of larval development. In: McEdward L, editor. Ecology of Marine Invertebrate Larvae. Boca Raton: CRC Press, Inc.; 1995. pp. 49–77.
- 45. Byrne M, Sewell MA, Prowse TAA. Nutritional ecology of sea urchin larvae: Influence of endogenous and exogenous nutrition on echinopluteal growth and phenotypic plasticity in Tripneustes gratilla. Funct Ecol. 2008;22: 643–648.
- 46. Reitzel AM, Miles CM, Heyland A, Cowart JD, McEdward LR. The contribution of the facultative feeding period to echinoid larval development and size at metamorphosis: A comparative approach. J Exp Mar Bio Ecol. 2005;317: 189–201.
- 47. Prowse TAA, Sewell MA, Byrne M. Fuels for development: Evolution of maternal provisioning in asterinid sea stars. Mar Biol. 2008;153: 337–349.
- 48. Sinervo B, McEdward LR. Developmental consequences of an evolutionary change in egg size: An experimental test. Evolution (N Y). 1988;42: 885.
- 49. Caballes CF. The role of chemical signals on the feeding behavior of the crown-of-thorns seastar, Acanthaster planci (Linnaeus, 1758). MSc Thesis. University of Guam. 2009.
- 50. Pratchett MS. Influence of coral symbionys on feeding preferences of crown-of thorns starfish Acanthaster planci in the western Pacific. Mar Ecol Prog Ser. 2001;214: 111–119.
- 51. Lucas JS. Reproductive and larval biology of Acanthaster planci (L.) in Great Barrier Reef Waters. Micronesica. 1973;9: 197–203.
- 52. Marsh JA. Primary productivity of reef-building calcareous red algae. Ecology. 1970;51: 255–263.
- 53. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9: 671–675. pmid:22930834
- 54. Ayukai T, Keesing JK, Cartwright CM, Halford AR, Hocking D, Okaji K. Practical handbook for large scale rearing of the larvae and juveniles of crown-of-thorns starfish. AIMS Report. 1996.
- 55. Byrne M, Barker MF. Embryogenesis and larval development of the asteroid Patiriella regularis viewed by light and scanning electron microscopy. Biol Bull. 1991;180: 332–345.
- 56. Anderson MJ, Gorley RN, Clarke KR. PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods. Plymouth, UK: PRIMER-E Ltd.; 2008.
- 57. Benjamini Y, Hochberg Y. Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Stat Soc Ser B. 1995;57: 289–300.
- 58. Henderson JA. Preliminary observations on the rearing and development of Acanthaster planci (L) (Asteroidea) larvae. Fish Notes, Queensl Dep Harbours Mar. 1969;3: 69–79.
- 59. Hoegh-Guldberg O, Pearse JS. Temperature, food availability, and the development of marine invertebrate larvae. Am Zool. The Oxford University Press; 1995;35: 415–425.
- 60. Wolfe K, Graba-Landry A, Dworjanyn SA, Byrne M. Larval phenotypic plasticity in the boom-and-bust crown-of-thorns seastar, Acanthaster planci. Mar Ecol Prog Ser. 2015;539: 179–189.
- 61. Mortensen T. Contributions to the study of the development and larval forms of echinoderms I-II. Det K Danske Vidensk Selsk Skr Naturvidenskabelig og Math Afd Ser 9. København: Blanco Lunos; 1931;4: 1–286.
- 62. Yamaguchi M. Larval behavior and geographic distribution of coral reef asteroids in the Indo-West Pacific. Micronesica. 1977;13: 283–296.
- 63. Hayashi R, Komatsu M, Oguro C. Wrinkled blastula of the sea star, Acanthaster planci (Linnaeus). Proc Japanese Soc Syst Zool. 1973;9: 59–61.
- 64. Mortensen T. Contributions to the study of the development and larval forms of echinoderms IV. Det K Danske Vidensk Selsk Skr Naturvidenskabelig og Math Afd Ser 9. København: A.F. Hølst & Søn; 1938;7: 1–59.
- 65. Yamaguchi M. Larval life span of the coral reef asteroid Gomophia egyptiaca. Micronesica. 1974;10: 57–64.
- 66. Yamaguchi M, Lucas JS. Natural parthenogenesis, larval and juvenile development, and geographical distribution of the coral reef asteroid Ophidiaster granifer. Mar Biol. 1984;83: 33–42.
- 67. Keesing JK, Lucas JS. Field measurement of feeding and movement rates of the crown-of thorns starfish Acanthasther planci (L.). J Exp Mar Bio Ecol. 1992;156: 89–104.
- 68. Keesing JK. Feeding biology of the crown-of-thorns starfish, Acanthaster planci (Linnaeus). PhD Thesis. James Cook University. 1990.
- 69. Cole AJ, Pratchett MS. Inter-specific variation in susceptibility to grazing among common reef corals. Mar Ecol Prog Ser. 2011;422: 155–164.
- 70. Hughes TP. Skeletal density and growth form of corals. Mar Ecol Prog Ser. 1987;35: 259–266.
- 71. Pearson RG, Endean R. A preliminary study of the coral predator Acanthaster planci (L.) (Asteroidea) on the Great Barrier Reef. Fish Notes, Queensl Dep Harbours Mar. 1969;3: 27–55.
- 72. Venable DL. Size-number trade-offs and the variation of seed size with plant resource status. Am Nat. 1992;140: 287–304.
- 73. Byrne M. The Echinodermata. In: Anderson DT, editor. Invertebrate Zoology. 2nd ed. Melbourne: Oxford University Press; 2001. pp. 374–402.
- 74. Bosch I, Colwell SJ, Pearse JS, Pearse VB. Nutritional flexibility in yolk-rich planktotrophic larvae of an antarctic echinoderm. Antarct J United States. 1991;26: 168–170.
- 75. Rappaport R, Rappaport BN. Cleavage in conical sand dollar eggs. Dev Biol. 1994;164: 258–266. pmid:8026628
- 76. Podolsky RD, Strathmann RR. Evolution of egg size in free-spawners: Consequences of the fertilization-fecundity trade-off. Am Nat. 1996;148: 160–173.
- 77. Patton JS, Abraham S, Benson AA. Lipogenesis in the intact coral Pocillopora capitata and its isolated zooxanthellae: Evidence for a light-driven carbon cycle between symbiont and host. Mar Biol. 1977;44: 235–247.
- 78. Stimson JS. Location, quantity and rate of change in quantity of lipids in tissue of Hawaiian hermatypic corals. Bull Mar Sci. 1987;41: 889–904.
- 79. Byrne M, Prowse TAA, Sewell MA, Dworjanyn SA, Williamson JE, Vaïtilingon D. Maternal provisioning for larvae and larval provisioning for juveniles in the toxopneustid sea urchin Tripneustes gratilla. Mar Biol. 2008;155: 473–482.
- 80. Boidron-Métairon IF. Larval nutrition. In: McEdward LR, editor. Ecology of Marine Invertebrate Larvae. Boca Raton: CRC Press, Inc.; 1995. pp. 223–248.
- 81. Meidel SK, Scheibling RE, Metaxas A. Relative importance of parental and larval nutrition on larval development and metamorphosis of the sea urchin Strongylocentrotus droebachiensis. J Exp Mar Bio Ecol. 1999;240: 161–178.
- 82. Podolsky RD, Moran AL. Integrating function across marine life cycles. Integr Comp Biol. 2006;46: 577–586. pmid:21672768
- 83. Suzuki G, Yasuda N, Ikehara K, Fukuoka K, Kameda T, Kai S, et al. Detection of a high-density brachiolaria-stage larval population of crown-of-thorns sea star (Acanthaster planci) in Sekisei Lagoon (Okinawa, Japan). Diversity. Multidisciplinary Digital Publishing Institute; 2016;8: 9.