(A) Summary table of GO Terms and component gene perturbation by time pre-seroconversion. Only GO terms and their genes with Bayesian score ≥|2.24| are included in analysis. (B-C) Heat maps of perturbed GO terms described by time pre-seroconversion and identified from the Early Phase (time –6, –5, –4, –3 pSN) (B) and the Later Phase (time –2, –1, 0, 1 pSN) (C). Red color indicates activation, green color indicates repression. Intensity of color represents amplitude of perturbation. The list of GO terms shown represent a subset of all perturbed terms that were selected as being most relevant to innate and adaptive immune response.

https://doi.org/10.1371/journal.pone.0156469.g001

(A) Visualization of sliding window correlation approach. (i) Hypothetical Log10(PRNT₈₀) data taken from time points capturing neutralizing antibody levels during time period in which animals reached threshold for protection (log10 = 1.903) (dark green bars). (i-ii)The trajectory of the Log10(PRNT₈₀) data is applied to other time points prior to serum neutralization (light green bars) to identify pathway with complementary or antithetical trajectories (A-ii for hypothetical Pathway Bayesian Z-score data, dark blue bars). (iii) Graphical representation of the R correlation coefficient value between Log10(PRNT₈₀) and Pathway Bayesian Z-score. (B) Pathways correlated to PRNT₈₀values at the time of seroconversion listed by pathway at incremented window times (each consisting of three time points), with significant correlation p-values (i), or plotted as log(PRNT₈₀) vs normalized Bayesian Z-score (ii) for six selected pathways having highest correlations.

https://doi.org/10.1371/journal.pone.0156469.g002