Birth synchrony is well documented among ungulates and is hypothesised to maximize neonate survival, either by minimizing the risk of predation through predator swamping or by synchronising birthing with increased seasonal food availability. We used encapsulated vaginal implant transmitters to locate and capture neonatal moose calves and document the seasonal and diel timing of parturition in two adjacent study areas with different predation pressure in central Ontario, Canada. We tested the hypothesis that predation promotes earlier and more synchronous birth of moose calves. Across both areas, proportionately more births occurred during the afternoon and fewer than expected occurred overnight. Mean date of calving averaged 1.5 days earlier and calving was also more synchronous in the study area with heavier predation pressure, despite average green-up date and peak Normalized Difference Vegetation Index date occurring 2 days later in this study area than in the area receiving lighter predation pressure. We encourage analysis of data on timing of parturition from additional study areas experiencing varying degrees of predation pressure to better clarify the influence of predation in driving seasonal and diel timing of parturition in temperate ungulates.
Citation: Patterson BR, Mills KJ, Middel KR, Benson JF, Obbard ME (2016) Does Predation Influence the Seasonal and Diel Timing of Moose Calving in Central Ontario, Canada? PLoS ONE 11(4): e0150730. https://doi.org/10.1371/journal.pone.0150730
Editor: Cheryl S. Rosenfeld, University of Missouri, UNITED STATES
Received: December 30, 2014; Accepted: February 18, 2016; Published: April 15, 2016
Copyright: © 2016 Patterson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All data used in the analyses are either publicly available (e.g. NDVI data), reproducible from figures (e.g., frequency data presented in Figs 2 & 4) or provided in Supporting Information (S1 Table).
Funding: Major funding for this work was provided by the Ontario Ministry of Natural Resources & Forestry through Ontario Parks and the Wildlife Research and Monitoring Section. Additional funding was provided by the Natural Sciences & Research Council of Canada (STPGP 321950-05 awarded to Dennis Murray). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Birth synchrony is well documented among ungulates [1, 2, 3, 4, 5] and is generally hypothesised to maximize neonate survival, either by minimizing the risk of predation through predator swamping [6, 7, 8, 9] or by synchronising birthing with increased seasonal food availability [10, 11, 12]. The timing of parturition, independent of synchrony, may also influence the survival of neonates. Early-born neonates may experience reduced predation relative to later born individuals by achieving greater size and reduced vulnerability to predators by the time predators recognize the birth pulse and begin to focus on the present year’s cohort of neonates [13, 14]. Alternatively, Keech et al.  provided evidence that moose timed parturition to maximize the use of the growing season, as previously suggested by Bowyer et al. . These authors suggested that moose time parturition as early as possible following winter to maximize the growing season for calves and speculated that young moose born too late in spring would have insufficient time to accrue body reserves necessary to survive harsh conditions in winter [10, 14]. Indeed, late born calves tend to be smaller in autumn and winter than early born calves .
Despite the potential influence of timing of parturition on calf survival, most studies have failed to detect an influence of inter-annual variation in environmental conditions on timing of moose parturition [16, 17]; but see also . In fact, although intraspecific variation in the timing of mating has been documented for some ungulate populations [1, 2, 15] the timing of mating and calving among North American moose (Alces alces) populations exhibits relatively low variation across >15° of latitude [16, 17].
There is ongoing debate regarding the relative importance of various selective pressures in shaping timing and synchrony of ungulate births within and among species. As part of a broader assessment of moose viability near the southern extent of their range in Ontario [18, 19, 20], we documented the seasonal and diel timing of parturition in 2 adjacent study areas. During a companion study of calf mortality, we documented a marked difference in predation rates on calves between the two areas (23.3% versus 9.0%; ). Here, we tested the hypothesis that predation promotes more synchronous, and earlier, birth of moose calves. Specifically, we predicted that mean date of birthing would be earlier and more synchronous (i.e. less variable) in the area receiving greater predation pressure, despite this area being subject to a later date of green up (see discussion). Additionally, although seasonal synchrony in birthing has been well documented, the recent advent of vaginal implant transmitters (VITs) containing coded signals that indicate the precise timing of expulsion (e.g. [22, 23, 24]) enabled us to objectively determine diel timing of parturition. Accordingly, we further predicted that if predation influenced the timing of parturition, proportionately more births would occur during the day-time when wolves (Canis spp.) and American black bears (Ursus americanus) tend to be less active [25, 26, 27, 28, 29].
Materials and Methods
We studied moose in 2 nearby study sites in central Ontario, Canada (45°N, 78°W)–one in the western portion of Algonquin Provincial Park (APP) (2,000 km2) and the other in the southeastern portion of Wildlife Management Unit 49 (WMU49; 1,500 km2) (Fig 1). The overall study area was near the southern distribution of moose range in Ontario , and lay within the northern portion of the Great Lakes-St. Lawrence Forest Region . The two study sites were separated by roughly 50 km, with western APP consisting of a protected forest with limited forest management and no moose harvest, whereas WMU49 included public and private lands where both logging and moose harvest occurred. Forest cover in APP was dominated by sugar maple (Acer saccharum), poplar (Populus spp.), American beech (Fagus grandifolia), yellow birch (Betula alleghaniensis), eastern hemlock (Tsuga canadensis), spruce (Picea spp.) and fir (Abies spp.); the forest composition in WMU49 was similar, although with lower abundance of hemlock and more developed and agricultural land and habitat fragmentation . The study sites differed in elevation, with APP (320–580 m ASL) being approximately 200 m higher than WMU49 (73–549 m ASL). Environmental conditions during our study were typical for the area. The long term normal for daily temperatures in January and July averaged -11°C and 18°C, respectively for APP (East Gate Station: 45° 31.8’N 78° 16.2’W) and about 1°C warmer during winter and summer in WMU49 (Huntsville: 45° 20.2’N 79° 13.4’W; ). Potential predators of moose in the region included eastern wolves (Canis lycaon), eastern coyotes (Canis latrans var.) and American black bears. Wolves were relatively abundant in APP (2.3–3.0/ 100 km2 ) but uncommon in WMU49 where most free-ranging canids were smaller eastern coyotes and wolf-coyote hybrids . Black bear densities were estimated at 37 bears/100 km2 (95% CI 21–66) and 32 bears/100 km2 (95% CI 15–57) for WMU49 and APP respectively .
The 2 study sites are hatched.
During 2007–2009 we used encapsulated vaginal implant transmitters (VITs, model M3970, Advanced Telemetry Systems, Inc Isanti, MN 55040 USA) to locate and capture calves from a random sampling of cows in both study areas. From January through March, 2007–2009 we captured 119 different adult female moose via darting or aerial net gunning and fitted them with either VHF or GPS radiocollars (see [18, 20]; Lotek 3300L, Lotek Wireless Inc., Newmarket, ON, Canada; Telemetry Solutions Quantum 5000; 5000b, Telemetry Solutions, Concord California, USA). We chemically immobilized moose in 2007 and 2008 by remote delivery of capture darts from a helicopter using a mixture of carfentanil (Wildlife Pharmaceuticals Inc., Ft. Collins, Colorado, USA) at approximately 0.0070 mg/kg combined with xylazine hydrochloride (Rompun®; Bayer Inc., Etobicoke, Ontario, Canada) at approximately 0.2 mg/kg . We reversed this drug combination with naltrexone at approximately 0.7 mg/kg. In 2009 all captured cows were net-gunned from a helicopter (Pathfinder helicopters, Salt Lake City, Utah, USA) and manually restrained without use of chemical immobilization.
We fitted 73 of these captured adult females with VITs on 91 occasions (i.e., 18 moose were fitted with VITs during multiple years). Prior to deployment, VITs were cold sterilized for 24h in Nolvasan solution, rinsed with sterile saline, and stored individually in sterile Whirl-Pak® bags. Each VIT was placed in a sterile disposable, lubricated speculum (Animal Reproductive Systems, Chino California, USA) which was then inserted in the vagina to the cervix. VITs were held in place at the cervix by a 19 mm diameter PVC tube as the speculum was slowly pulled back. Disposable speculums were discarded, and the smaller diameter PVC tube washed with soap and water and cold sterilized in Nolvasan solution between deployments. VITs were typically shed from moose at parturition and the pulse rate changed from 40 PPM to 80 PPM when the temperature dropped below 32°C. We recovered most expelled VITS from within or immediately proximate to obvious calving beds and were only aware of two cases were VITs were expelled prior to the onset of parturition. In addition to the pulse rate change, the VITs emitted a coded pulse that enabled us to determine how long each had been shed, in increments of 30 minutes, for periods of up to 5.5 days. During the course of the study we documented a few cases where VITs had their temperature sensitive mortality switches reset by exposure to direct sunlight. VITs that had been reset by the sun were detected through repeat monitoring. For example, in rare cases we would discover that a VIT known to have been out for multiple days was suddenly sending a signal indicating more recent expulsion. Checking the timing code for VITs that had been “reset” in this manner indicated they had all been reset during the warmest part of the day and this was only ever documented for VITs shed in open meadows and exposed to direct sunlight–whereas most calving sites were in forested cover (A. McLaren and B. R. Patterson, unpublished data) where resetting of the timer was not a risk.
To avoid pseudoreplication, only a single calving date was recorded in cases where twinning was documented. All capture and handling methods followed the guidelines of the American Society of Mammalogists  and were approved by the Trent University Animal Care Committee as well as the Animal Care Committee of the Ontario Ministry of Natural Resources (OMNR; Permit Nos. 07–66, 08–66, 09–66). The OMNR permits were required to handle moose in both study areas; additionally we were authorized by Ontario Parks to conduct this research in Algonquin Park. No other permits or authorizations were required to conduct this research and our research did not involve endangered or protected species.
We aerially radio-tracked moose that had been fitted with VITs biweekly following capture until early-May, when daily monitoring began. At the onset of calving these moose were checked daily from the air or by a ground crew to determine calving status. When a VIT was shed, a crew of 2–4 located the calving site using a hand-held antenna and portable receiver. Calf handling and subsequent monitoring are described elsewhere .
We used a 2-tailed t-test assuming unequal variances to test whether mean calving dates differed between APP and WMU49. We used this test because it is robust to slight departures from normality , and the distribution of calving dates was normal in APP (Ks = 0.102, P = 0.50) and only slightly skewed in the right tail in WMU49 (Ks = 0.134, P = 0. 035; Fig 2). Although a 1-tailed test would have been more appropriate for testing the specific hypothesis that calving occurred earlier in APP owing to greater predation pressure, earlier calving in WMU49 owing to an earlier mean date of green up was also a possibility, hence the 2-tailed test. Synchrony of births is best quantified as the variance around birth date [38, 39]. Therefore, we used a 2-sample F-test to determine differences in variance of birth dates between the 2 areas. To test for diel patterns in the timing of parturition we used circular statistics to find the mean time of day of calving, and used Rayleigh’s z test for circular uniformity to test the null hypothesis of a uniform distribution. Small sample sizes precluded our testing for differences in diel timing of births between study areas. All Statistical testing was conducted using R (Version 2.15.3; ).
We successfully recorded the date of parturition of 77 calves born to cows fitted with VITs (28 in APP and 49 in WMU49; Table 1). We censored one outlying birth date in WMU49 (June 3) as this likely represented conception during second estrous and the date was > 6 standard deviations later than the mean date. In APP, an additional 44 neonatal calves were encountered without the use of VITs. However, the mean date of encountering these calves (May 17) was approximately 5 days later than the mean parturition date determined for this study area using VITs (May 12) and many of these calves were estimated to be >72 hours old upon encounter. To avoid biasing our estimate of mean parturition date in subsequent analyses we included only data from 9 calves (including 2 sets of twins, so n = 7 additional data points) encountered without use of VITs that we were confident were < 24 hours old at time of encounter. This determination was based published criteria  and experience gained while observing the calves born to VITted females at various time periods following their birth. Our observations corroborated those of Larsen et al.  that “.a calf < 1 day old was unable to stand, or was unsteady on its feet, or the umbilical cord was still attached, or the calf was still wet;.” In our experience a calf that was still wet or had a wet umbilical cord attached was < 12 hours old. Based on those criteria we were not only confident that each of the 9 calves in question was <24 hours old at time of discovery, but also in our ability to determine the most likely day of their birth based on their estimated age and the time of day the assessment was conducted. These additional calves increased the sample size of known parturition dates in APP to 35 (Table 1).
Across both study areas the first date of successful calving was May 8, with an overall mean date of calving of May 13 (Table 2). Eighty percent of calves were born by May 16 each year (Fig 2). Although small sample sizes precluded rigorous testing for inter-annual variation in parturition dates, visual inspection of the data suggested that the distribution of calving dates was similar from 2007–2009 (Table 2). From 2007–2009, mean date of calving averaged 1.5 days earlier in APP than WMU49 (t = -2.25, df = 81, P = 0.013). Calving was also more synchronous in APP, with calving dates being significantly more variable in WMU49 (variance = 12.4 versus 5.72; F34, 47 = 0.463, P = 0.010).
Based on the coded pulse emitted by the VITs, and excluding cases where field personal did not record the coded signal indicating time of expulsion, or where warm temperatures apparently reset the coded timer in the VITs, we recorded precise time of birth for 39 moose calves (18 and 21 in APP and WMU49, respectively). The timing of birth was not uniform throughout the 24–hr period; mean calving time was 14:49 with an angular dispersion of r = 0.299 (Z = 3.486, P = 0.0294; Fig 3).
The median parturition dates we documented (May 12 in APP (mean = 12.4), May 13 in WMU49 (mean = 13.9)) were earlier than documented in northwestern Ontario (May 14–18; ), Québec (May 18–June 8; ), or the interior of Alaska, USA (May 20–27; [10, 44, 45]). The median parturition dates we documented were also considerably earlier than the median dates of first observation of cows with neonates in part of our APP study area during 1981–84 (May 18–20; ). However, the observations by Addison et al.  were restricted to islands in APP, used by an estimated <20% of calving moose in the park, and first observation of cows with calves may not accurately reflect the actual day of parturition, as suggested by the portion of our data on age of captured moose calves for which we used similar capture techniques as Addison et al. . We suggest that the use of VITs deployed in a random sample of cows in winter provided a less biased measure of parturition dates for moose in our study areas.
We also observed later and less synchronous parturition of moose calves in the study area with lighter predation pressure (WMU49) than observed in nearby APP which, at only 50 km to the east, had similar habitat and climatic features. Wolves and black bears were the only significant predators of moose during this study. Our assertion that predation pressure was lighter in WMU49, despite potentially higher bear densities there, was based primarily on the results of a companion study of calf mortality ; however, other lines of evidence also suggest this was the case. Wolves were relatively abundant in APP, and although wolf-like canids were likely more abundant in WMU49, most free-ranging canids in the area were smaller eastern coyotes and wolf-coyote hybrids  that are less likely to prey on moose calves (e.g. ).
Among black bears, large males may be more likely to prey on ungulates [48, 49], including moose calves , and although bear densities were similar between areas the population in WMU49 was hunted. Harvest of bears across Ontario is skewed towards males, especially subadult males . Therefore, hunting in WMU49 may have altered both the sex and age ratios of the bear population relative to that in APP such that there were fewer large male bears capable of successfully preying on moose calves in the area. Adult female black bears do prey on moose calves, but such predation attempts are potentially risky and a defensive cow moose can be dangerous prey for much smaller adult female black bears . Such predation attempts are likely less risky for the much larger adult male black bears, and adult male black bears can even prey successfully on adult cow moose , so a black bear population with a lower proportion of adult males would be expected to exert less predation pressure on moose.
That predation pressure differed between the two areas was also consistent with the marked differences in defensive behavior exhibited by cows in the 2 study areas. Maternal defense of calves was almost absent in WMU49, with cows generally retreating when they first observed our approach to their calf . In APP, maternal defense became increasingly common during the course of our study, to the extent that by 2008 we were unable to handle the calves of cows without first chemically immobilizing the cows because of strong aggression . Although the lack of maternal defensive behavior in WMU49 may also reflect a learned flight response to hunting by humans occurring in this study area but not APP, hunted moose in other jurisdictions experiencing significant predation on calves exhibit similarly aggressive defense of their calves (e.g., ) suggesting that higher rates of predation on calves in APP was a more likely cause of this defensive behavior.
Algonquin Provincial Park was established as a protected area in 1893. However, given that wolves were lethally controlled in APP until 1958  it is not clear how long predation pressure by wolves on moose calves has been appreciably different between the 2 study areas. Regardless, it may seem difficult to imagine measurable shifts in the timing and synchrony of births by moose in such a short period of time (i.e., since the establishment of APP as a protected area in 1893, or since the establishment of wolf protection in APP in 1958). However, moose captured in the Northern Hemisphere and introduced to New Zealand quickly adjusted their timing of births to occur in spring in New Zealand, which was 6 months different from their capture location . Similarly, bighorn sheep (Ovis canadensis) captured from established populations in Utah, Alberta, Montana, and Colorado and translocated into two separate populations in northern Utah adjusted timing and synchrony of parturition to the environmental conditions of their release sites within 5 years of reintroduction .
The mean calving date in APP was only 1.5 days earlier than in WMU49, and similar to the discussion above, some might find it difficult to envision such a subtle difference in parturition date conferring much of a selective advantage in terms of predator avoidance. However, calves during our study gained mobility quickly after birth and by ca. 3 days of age were often difficult to capture by hand on the ground . Furthermore, in an area of south-central Alaska that received heavy predation pressure, moose calves that survived to autumn (n = 86) were born an average of 1.8 days earlier than the mean calving date for the overall population (n = 248 calves ). As discussed by Testa , the selective force of predation on birth dates over generations is expected to be cumulative and the optimum birth date in any given year is always earlier than that actually occurring. Over time, the selective pressure of predation should push heritable variation in timing of parturition to the earliest possible date until balanced out by opposing selective forces (e.g. timing of green-up [9, 10]).
Bowyer et al.  noted that extirpation of wolves and grizzly bears (Ursus arctos) from much of their original distribution in North America has produced a patchy distribution in the primary predators of moose across the continent. They suggested that the continental synchrony in onset and duration of parturition among North American moose, despite spatially variable predation pressure, indicates moose are tracking long-term patterns of climate to time reproduction . However, the observed differences in timing and synchrony of parturition observed between our 2 study areas were not likely due to differences in climatic conditions or plant phenology because temperatures were actually slightly warmer in WMU49 [32, 56]. Further, between 2007 and 2009, average green-up date and peak NDVI date occurred two days earlier in WMU49 compared to APP (13 April and 15 April, and 3 and 5 July, respectively ; yet moose calved later and less synchronously in WMU49. We also discount differences in the genetic composition or nutritional plane of moose between the 2 study areas as an explanation for some or all of the differences in parturition we observed. Although the genetic composition of a population has been implicated as a driver for timing and synchrony of births in reintroduced bighorn sheep , moose in our two study areas exhibited no genetic differences . Pregnancy rates, blood-based condition indices , twinning rates , and weights of calves <36 hours old at time of handling (APP = 15.5 kg, n = 18; WMU49 = 14.9 kg, n = 30; t 18, 30 = 0.880, P = 0.383) were all similar between areas indicating no apparent differences in condition of females between areas.
Another alternative hypothesis warranting consideration relates to maternal age. On the island of Vega in northern Norway, younger female moose gave birth later than older females [15, 60]. Based on analysis of cementum annuli in extracted vestigial teeth of cow moose captured during our study (n = 84) the mean age of cows from APP was 4.8 ± 0.42 versus 4.0 ± 0.40 years in WMU49 (; Fig 4). However, the difference in age distributions between the 2 areas was largely a result of substantially more yearling cows in WMU49 (Fig 4), probably owing to the higher calf survival documented for this area . Also, all of the known yearling female moose radiocollared during this study were captured in winter 2006 (7 in APP, 2 in WMU49), a year before the present study began, meaning all female radiocollared moose recruited into the study in 2007 would have been at least 2 years old. Beginning in 2007 we explicitly instructed capture crews to focus on mature female moose, and none of 15 newly captured and aged cows in 2007 (8 and 7 in APP and WMU49, respectively) were yearlings. We did not section teeth for aging in 2008 or 2009 but based on crew instruction and pictures of the captures we believe few if any yearlings were included in the samples of parturient cows during these years. Regardless, given that differences in birthing dates with age are usually driven by the first few primiparous age classes (>2 years old for moose; [15, 60, 61]), and that the distribution of these age classes was essentially the same between the two study areas even when the 2006 captures are considered (Fig 4), we suggest there were insufficient differences in age structure between the two study areas to drive the earlier parturition dates in APP.
Diel timing of births
Although birth dates are well documented for many ungulate species, given the recent advent of VITs, there are few data on precise diel timing of births. Small sample sizes precluded our testing for differences in diel timing of births between study areas, but in pooling study areas we documented a disproportionate number of births in the afternoon. Among mammals the onset of labour is immediately preceded by a sharp increase in free circulating oestrogens . The proximate triggers of this increase in free circulating oestrogens are not definitively known, but cortisol is also involved in the process and Nathanielsz  suggests that animals tend to give birth when they feel the most secure and comfortable. Changes in plasma cortisol levels likely represent the link between stress related to perceived predation risk and the cascading hormonal changes that may influence the onset of labour [62, 63].
Throughout much of the year wolves tend to be less active during mid-day and most active during crepuscular and dark periods [26, 27, 29]. Though diel activity patterns of black bears are more variable across their range, they often have reduced activity levels during the mid-day period and a spike in activity in early evening [25, 28]. Thus moose may be less stressed and more likely to enter labor at the time of day when they are more likely to be resting and least likely to be discovered by wolves or bears. We note however that our data provide only weak support for this hypothesis given that our pooled sample of birth times contained slightly more data from WMU49 where predation pressure was relatively light. This suggests either that several factors are influencing this trait and/or that there is not strong selection against having calves during the afternoon such that the trait persists even in areas with relatively light predation pressure. Nonetheless, predation risk clearly influences other aspects of moose life history [64, 65] and Addison et al.  previously suggested that predator avoidance likely exerted a strong influence on birthing site selection by moose in APP. Accordingly, it seems reasonable that predation risk may also influence the diel timing of parturition.
Alternatively, the disproportionate number of afternoon births may simply reflect selection for birthing during the warmest time of day, as temperatures occasionally reached freezing over night during calving in this study and neonatal calves can be sensitive to exposure during harsh weather [67, 68]. However, Bowyer et al.  were dismissive of the temperature hypothesis based on the large body size of moose calves relative to other smaller-bodied ungulates born under similar conditions. Indeed, non-predation-related mortalities are rare among neonatal moose calves in Alaska and the Yukon [41, 69]. Finally, a disproportionate number of afternoon births may simply reflect the fact that moose themselves tend to be less active at this time of day, potentially favouring the onset of labour [62, 63]. Additional investigations of calving by captive and free-ranging moose are required to clarify the potential influence of predation risk in driving the seasonal and diel timing of parturition.
S1 Table. Date and time of day of parturition for moose calves in central Ontario Canada, 2007–2009.
This study was part of a larger assessment of moose viability funded primarily by a Strategic Natural Sciences and Engineering Research Council of Canada (NSERC) grant, and an equipment grant from the Canadian Foundation for Innovation (CFI), both granted to Dennis Murray, Trent University, Peterborough, Ontario. Primary funding for the work described here was provided by the Ontario Ministry of Natural Resources & Forestry, through Ontario Parks and the Wildlife Research and Monitoring Section. Andrew Silver and Mark Gibson assisted greatly with logistical arrangements related to calf captures. Erica Newton assisted with the calculation of NDVI values for our study areas and Sarah Jamieson assisted with the literature search regarding onset of labor. We further thank the many students, volunteers and technicians who participated in field searches for moose calves. Art Rodgers and J. Ward Testa provided useful comments on a previous draft of the manuscript.
Conceived and designed the experiments: BRP KJM KRM JFB MEO. Performed the experiments: BRP KJM KRM JFB MEO. Analyzed the data: BRP KRM. Wrote the paper: BRP KJM KRM JFB MEO.
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