We present here the first cases in Neandertals of congenital clefts of the arch of the atlas. Two atlases from El Sidrón, northern Spain, present respectively a defect of the posterior (frequency in extant modern human populations ranging from 0.73% to 3.84%), and anterior (frequency in extant modern human populations ranging from 0.087% to 0.1%) arch, a condition in most cases not associated with any clinical manifestation. The fact that two out of three observable atlases present a low frequency congenital condition, together with previously reported evidence of retained deciduous mandibular canine in two out of ten dentitions from El Sidrón, supports the previous observation based on genetic evidence that these Neandertals constituted a group with close genetic relations. Some have proposed for humans and other species that the presence of skeletal congenital conditions, although without clinical significance, could be used as a signal of endogamy or inbreeding. In the present case this interpretation would fit the general scenario of high incidence of rare conditions among Pleistocene humans and the specific scenariothat emerges from Neandertal paleogenetics, which points to long-term small and decreasing population size with reduced and isolated groups. Adverse environmental factors affecting early pregnancies would constitute an alternative, non-exclusive, explanation for a high incidence of congenital conditions. Further support or rejection of these interpretations will come from new genetic and skeletal evidence from Neandertal remains.
Citation: Ríos L, Rosas A, Estalrrich A, García-Tabernero A, Bastir M, Huguet R, et al. (2015) Possible Further Evidence of Low Genetic Diversity in the El Sidrón (Asturias, Spain) Neandertal Group: Congenital Clefts of the Atlas. PLoS ONE 10(9): e0136550. https://doi.org/10.1371/journal.pone.0136550
Editor: David Frayer, University of Kansas, UNITED STATES
Received: June 12, 2015; Accepted: August 5, 2015; Published: September 29, 2015
Copyright: © 2015 Ríos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: Fossils are from the "El Sidrón (Asturias, Spain) Neandertal site. The fossils are under study at the Museo Nacional de Ciencias Naturales, Madrid, Spain. Interested researchers may contact the authors, or contact Gobierno del Principado de Asturias, Patrimonio Histórico y Cultural Plaza Sol, 8, 33009 Oviedo (Asturias).
Funding: Funding was provided by Ministerio de Economía y Competitividad, Spain, projects CGL2012-36682 and CGL2012-37279. Convenio Principado de Asturias-Universidad de Oviedo CN-09-084. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
A better understanding of the process of origin, change and extinction of Homo neanderthalensisis accomplished through the study of its population dynamics. This objective can now be addressed through paleodemographic studies based on osteological data from the fossil record , through analysis of Neandertal genetic data [2, 3] and through a combination of statistical methods considering both kinds of information [4, 5].Besides this approach, based on gathering available data from chronologically and geographically dispersed fossils, it is also informative to investigate the structure and dynamics of groups composed of contemporaneous individuals, which represent a more fundamental unit of genetic and social organization of Neandertal populations. The peculiarities of the El Sidrón site (Asturias, Northern Spain), allow us to address these questions.
The El Sidrón Neandertal assemblage is composed of more than 2400 human fossils recovered ina secondary context in a karst system [6, 7], and dated to 49000 years ago. A minimum number of thirteen individuals have been identified in this sample, including seven adults, three adolescents, two juveniles and one infant, with representation of all skeletal regions[9, 10].The genetic analysis of the sample indicated the presence of a small patrilocal community with low genetic diversity , corroborating previous archaeological and geological data that pointed to a contemporaneous social Neandertal group. Thus, besides genetic studies associated with diverse functional traits in Neandertals (i.e. pigmentation, blood group, language and taste perception) , the presence of this assemblage has allowed us to address important questions at the intraspecific and intra-group levels. The analysis of dental calculus has revealed evidence of cooking and plant use, further studies of the dentition have addressed sexual division of labor  and handedness, and morphological variability has been studied in different bone elements at the cranial [16, 17] and postcranial[18, 19]levels.
The presence of a contemporaneous Neandertal group with close genetic relations, together with recent studies in Neandertalpaleogenetics pointing to long-term small and decreasing population size with reduced and isolated groups , opens the possibility to explore the presence of skeletal traits related to kinship , and/or considered as potential signals of inbreeding[22, 23], supplementing genetic research in Neandertals regarding their biological variability and demography. In this regard, evidence of a retained deciduous mandibular canine, a condition with a probable familial basis, was reported for two dentitions from El Sidrón. We present here the study of congenital clefts in the first cervical vertebrae within this groupand discuss its potential implications for Neandertal demography.
Material and Methods
SD-1643 is an almost complete atlas reconstructed from three bony elements (Fig 1). It only lacks the right lamina or right posterior arch of the atlas due to a post-mortem fracture (transversal, regular surface of breakage with exposure of trabeculae) observed immediately posterior to the right lateral mass. The left lamina is truncated at the sagittal midline, the location of the posterior synchondrosis of the atlas (hereafter PS).
(A) Superior view of SD-1643, bar represents 1 cm. (B) Left transverse foramen completely closed, bar represents 1 cm. (C) Visible epiphyseal surface on the tip of the left transverse process. (D) Medial view of the tip of the left lamina, where no bone breakage and a smooth continuity of the cortical bone can be observed. (E) ESEM image, detail of the tip, with continuous cortical bone. (F) ESEM image, detail of another area of the tip with continuity of the cortical bone along its edge.
SD-1094 is a 33.1 mm length fragment whose location corresponds to the right anterolateral quadrant of a first cervical vertebra. The anterior half of the superior and inferior articular surfaces, as well as the facet for the dens of the axis, are recognizable (Fig 2). The anterior arch of the atlas truncates approximately at the sagittal midplane, whereas during development the anterior arch presents two synchondrosis (hereafter AS), each one located symmetrically medial to the lateral masses of the atlas.
(A) Superior view of SD-1094, placed on its anatomical location within a modern human atlas, bar represents 1 cm. (B) Posterior view of SD-1094, the right superior and inferior articular surfaces can be observed. The facet for the dens of the axis can be observed in the anterior arch, bar represents 1 cm. (C) Anterior view of SD-1094, the beginning of the anterior tubercle of the transverse foramen can be observed on the left. (D) Medial anterior view of the midsagittal cleft, slightly tilted to superior in order to view its anterior inferior corner. Continuity of the cortical bone from the anterior to the sagittal surface can be observed. (E) ESEM image, with clearer view of the anterior inferior corner, with continuity of the cortical bone from the anterior to the sagittal surfaces. (F) Medial view of the anterior sagittal cleft. Cortical bone can be observed in the inferior and superior thirds. Bone breakage can be observed in the central third. (G) ESEM image, with clearer view of the cortical bone and the areas of bone breakage. (H) Medial posterior view with continuity of cortical bone from the posterior to the anterior surfaces of the posterior arch of the atlas. (I) ESEM image of the superior corner of the midsagittal cleft, with clearer view of the cortical continuity between the posterior and anterior surface along the sagittal cleft.
SD-1605/1595 is a complete adult atlas with no significant observable feature (Fig 3)
(A) Superior view of SD-1605/1595, bar represents 1 cm. (B) Radiography of SD-1605/1595.
An extant modern human subadult sample comprised of documented and archaeological skeletons was gathered for comparative purposes. The documented skeletons come from the Museu Nacional de Historia Natural of Lisbon (Portugal), and from the Museo Anatómico from the Faculty of Medicine of Valladolid (Spain), with a total of 47 skeletons with an age range from birth to 14 years old. These known sex and age skeletonscomprised the skeletal documented sample (SDS). The 25 archaeological skeletons, ranging in dental age from 3.5 to 11.5 years, come from three different collections: Leiria (Portugal, 13th to 16thCenturies) , Lagos (Portugal, 15th to 17th Centuries) ,and Baza (Spain, 11th to 16th Centuries) . The degree of closure of the AS and PS, and the transverse foramen (hereafter TF) of the atlas were recorded according to a three grade scale (0: absence of fusion; 1: active fusion; 2 complete fusion). Three adult human atlases with congenital clefts at the PS were collected for comparative purposes from the anatomical collection curated at the Anatomical Museum from the Faculty of Medicine, Valladolid (Spain).
The regions of interest of SD-1643, SD-1094 and selected modern human atlases were inspected under binocular lens and Environmental Scanning Electron Microscope (ESEM Fei-Quanta 200) located at the National Museum of Natural History (MNCN-CSIC). Selected atlases were examined at 25.0 kv accelerating voltage and low vacuum mode. The magnification observations ranged from 40x to 1000x. Conventional radiographs were taken of the three Neandertal atlases and selected modern human atlases.
Results and Discussion
The explanation of the truncation of the left lamina of SD-1643, and the anterior arch of SD-1094 is discussed via a differential diagnosis including normal-for-age lack of fusion of the PS and AS, ante-, peri- and post-mortem fractures, and congenital clefts of the arch of the atlas. Each of these possibilities is discussed separately.
Normal-for-age lack of fusion of PS and AS
A normal-for-age lack of fusion of the PS for SD-1643 is excluded based on three observations. First, a universal feature of the pattern of maturation of the atlas in humans is the fact that the PS fuses invariably before the two AS [29–33], except in some atlases with only one sagittal AS, which is not the case of SD-1643. Second, the continuity of the cortical surface along the tip of the lamina can be observed in the radiograph(Fig 4). Third, absence of a synchondrosis-like surface on the tip of the lamina can be observed, indicating that there was no cartilage bridging the left lamina to the right lamina and thus there was no actual synchondrosis (Fig 5).Additional observations can be added regarding the maturation and chronological age of SD-1643 by modern human references, based on the degree of closure of the AS, the TF, and the degree of maturation of the epiphyseal surface located in the tip of the left transverse process (Fig 1). With regard to the AS, diverse CT scan studies indicate that the earliest age of closure is reported to be at 3–4 years [31, 33], while the oldest age at which the AS is apparent ranges from 6.83 years to 7 years and 1 month  to 7 years and 3 months . Our own observations in the SDS indicate that the oldest case of lack of fusion of the AS was 5.8 years, active fusion was observed between 5.3 to 7.2 years, and the earliest case of complete fusion was 6.1 years. With regard to the TF, it has been stated that it is “usually near completion by years 3–4” . Our own observations in the SDS indicate that active formation of the TF was observed between 1.5 and 9 years, while the earliest case of complete TF was observed at 4.75 years. With regard to the epiphyseal surface of the tip of the transverse process, it has been observed that its presence indicates an age below 18 years, while the transformation of this surface into cortical bone indicates an age older than 15 years . A broad age range from 4.75 years to 18 years can thus be assigned to SD-1643 based both on the complete closure of the TF and on the presence of the epiphyseal surface in the tip of the transverse process.
(A) Radiography of SD-1643, with continuity of the cortical bone through the irregularly-shaped tip of the left lamina. (B) Radiography of SD-1094, the arrow indicates the continuity of cortical bone at the anterior inferior corner of the midsagittal cleft (see Fig 2D and 2E), while the triangle indicates the continuity of cortical bone at the superior corner of the cleft (see Fig 2H and 2I). Lack of continuation of cortical bone through the entire cleft is due to post mortem breakage (see Fig 2F and 2G).
(A)The PS of the atlas of a 3 year old skeleton from the anatomical collection curated at University of Valladolid. Cartilage was left in place in this preparation and it can be observed bridging both tips of the posterior arches. Numbers are located in an area of the synchondrosis where a small portion of the cartilage has been lost. Numbers indicate: 1, cortical bone; 2, synchondrosis surface; 3, cartilage. (B) ESEM image with detail of the aforementioned area. Smooth surface of the cortical bone can be observed on the left (1). The surface of the synchondrosis presents a uniform porous aspect formed by numerous and small pore-like structures (2). The cartilage presents a uniform opaque surface (3). (C) ESEM image with further detail of the image presented in B. The porous-like surface of the synchondrosis (2) can be clearly observed, associated to the anchorage of the cartilage. (D, E, F, G, H)The PS (left posterior arch) of non-fused atlases from modern human subadult skeletons. The limit between the cortical bone and the synchondrosis surface is very diverse as can be observed in this small sample, but the differentiation between both surfaces is clear.
With regard to SD-1094, from a maturation perspective the frequency of mid-sagittal anterior clefts of the atlas in subadult samples ranges from 0.7% , to 16.5% , to even 21.8% , (observations limited to ages below 7 years  or below a maximum of 12 years ), indicating that during the subadult period the presence of only one AS at the anterior sagittal midline represents a normal variant of the maturation of the anterior arch of the atlas. Frequency values for adult samples range from 0.087%  to 0.1% , a fact that indicates that most of the anterior clefts observed in the subadult samples eventually ossify before adulthood . Again, a normal-for-age lack of fusion of the AS for SD-1094 is excluded based on the continuity of the cortical bone and the absence of a synchondrosis-like surface on the midsagittal plane of the anterior arch, as observed in the ESEM and radiographic images (Figs 2, 4 and 5).
Fractures of the atlas
For SD-1643, the possibility of an ante-, peri- or post-mortem fracture is discarded based on several observations. Different classifications of fractures of the atlas in the living have been developed based on their location [39–44], but no one includes a single fracture at the posterior sagittal midline. This fracture can occur, but in all the cases reviewed it is accompanied by at least one other fracture generally at the anterior sagittal midline , a combination included in some of the aforementioned classification systems . Furthermore, as indicated above, radiography and observations through binocular lens and ESEM show two features: First, a clear smooth continuity of the cortex along the tip of the lamina of SD-1643, without exposure of the inner trabeculae, thus excluding peri- and post-mortem fractures; second, absence of osteogenic reaction thus excluding a healed but unfused ante-mortem fracture  (Fig 1).
With regard to SD-1094, fractures at the anterior sagittal midline have been described , but the augmented images again indicate a smooth continuity of the bony cortex from the ventral and dorsal surface of the anterior arch to the sagittal midplane (Fig 2). This continuity is only interrupted due to post-mortem erosion with exposed trabeculae in the central part of the midplane, and absence of osteogenic response compatible with a healed but unfused ante-mortem fracture is observed also for SD-1094 (Fig 2). These observations exclude ante-, peri- or post-mortem fractures as possible explanations for the presence of the sagittal midplane truncation of the anterior arch of the atlas of SD-1094.
Congenital clefts of the atlas
The above findings indicate that the truncation of the left lamina of SD-1643 at the sagittal midline correspond to a congenital defect that could be classified as type A according to the system of Currarino et al. . A comparison of SD-1643 with modern human cases with type A defects is presented in Fig 6. A type B defect is defined as a condition affecting only one lamina and ranging from a small gap to a complete absence of the lamina, and it cannot be excluded for SD-1643 due to postmortem absence of the right lamina (Fig 1). The frequency of type A defects ranges from 0.73%  to 3.84% , accounting for more than 77% of all of the defects reported for the posterior arch of the atlas [49–55] (Table 1).
Comparison between SD-1643 and three cases of a type A posterior cleft of the atlas from the anatomical collection curated at University of Valladolid (UVA). In this superior view the truncation of the anterior arches at the midsagittal line can be observed. As indicated in the text, the post mortem breakage immediately posterior to the right lateral mass of SD-1643 opens the possibility of a type A or type B (absence of one lamina) defect.
With regard to SD-1094, the above findings indicate that the truncation of the anterior arch at the sagittal midplane corresponds to a congenital cleft, in this case a very rare condition in adults according to previous work, with reported frequencies from 0.087% , to 0.09%  to 0.1% . It is also interesting to note that in most cases, an anterior cleft is associated with the presence of a posterior cleft resulting in a bipartite atlas [56–72]. With regard to clinical manifestations of defects of the arch of the atlas, they range from lack of symptoms even in cases of total aplasia of the posterior arch  to atlantoaxial instability  and cervical myelopathy (types C and D). Defects of the arch of the atlas have been also observed associated with conditions such as Down syndrome [76, 77], Chiari malformation , thalassemia minor , Klippel-Feil syndrome [80–82]and other skeletal dysplasias like Goldenhar syndrome, Conradi syndrome, and atlas assimilation . Focusing on the type A defect, most of the reported cases correspond to incidental findings during routine medical examination [37, 51, 83], with some exceptional cases of a type A associated with clinical manifestations (torticollis, facial asymmetry) . With regard to anterior clefts of the atlas, in most cases a bipartite atlas or an isolated anterior cleft again constitutes an incidental finding during clinical examination subsequent to trauma to head and neck [56, 57, 61–72, 85, 86], and only in a few cases is an anterior cleft of the atlas associated with clinical symptoms, for instance due to hyperostosis caused by hypertrophy of the anterior arch . Asrecently summarized , the review of the literature indicates that most of the bifid dorsal and ventral atlantal arches could be considered incidental findings, and thus likely no clinical symptoms would have been associated with the congenital clefts of SD-1643 and SD-1094.
Interestingly, defects of the arch of the atlas have been observed in two family groups, a mother (type B) and her sibling (type E) , and a mother (type A) and her two siblings (type E plus anterior cleft of the atlas in both cases) , in the latter case a condition compatible with autosomal dominant inheritance. At El Sidrón, a dental anomaly (retained left mandibular deciduous canine) with a potential heritable basis has been previously described for two individuals who presented the same mitochondrial haplotype , and in this regard statistically significant higher frequencies of type A defects of the atlas have been associated consistently with cleft palate [88, 89]and palatally displaced canines . Other dental anomalies (i.e. uneruption and transposition of teeth) have been incidentally associated with both anterior and posterior clefts of the atlas [91, 92]. The association between clefts of the atlas with dental and other cervical anomalies [60, 62, 64, 80], most likely indicates a disturbance common to the close periods of embryogenesis of these structures. In this regard, a thorough and explanatory embryology-based classification of bony malformations of the craniovertebral junction has been elaborated by Pang and Thompson , who indicate that malformations of the arch of the atlas emerge from disturbances of the lateral zone of the upper cervical sclerotome (posterior arch) and the hypochordal bow (anterior arch). Further research in the embryological origin and schedule of cervical and craniofacial structures would shed light on these associations.
Potential implications for Neandertal demography
We present here the first report of congenital clefts of the arch of the atlas in Neandertals. No other cases of such defects have been observed in studies of Neandertal cervical vertebrae which include those cases with best preservation of the atlas such as La Ferrassie 1 , Krapina 100–101[94, 95], and Regourdou 1 , or in studies of other well preserved hominin atlases . Based solely on the findings for the atlas presented here and on the previously reported cases of dental anomalies , the number of individuals from El Sidrón presenting congenital conditions would range from a minimum of two (Adolescent 3/SD-1643, an association supported by compatible dental and bone age; Adult 2/SD-1094), to a maximum of four (each atlas and tooth representing a different individual). The observation that at least two out of three observable atlases present low frequency congenital anomalies supports previous genetic  and dental  evidence from El Sidrón indicating that these Neandertals constituted a group with close genetic relations. Familial relationships have been previously proposed for human skeletal remains in archaeological  and forensic  contexts, based on the common presence of anatomical variants of low population frequency. Although most of the variants used in the detection of kin groups are dental traits, cranial  and postcranial variations including traits of the first cervical vertebra [100, 101] have been also reported to be associated to familial relations. But beyond the usefulness of these traits for recognition of kin groups, it has been suggested in conservation biology that some anomalies without direct effect on fitness (e.g. minor tooth anomalies and thoracolumbar and sacrococygeal transitional vertebrae), could be used as indication of inbreeding . In this regard, González-Reimers et al.  observed a bipartite atlas, an atlas with a type B defect, and two cervical blocks in a prehispanic ossuary of the island of El Hierro (Canary Islands), pointing to possible familial relations or to an unusually high prevalence of these conditions in this islander population. Similar observations have been reported by Merbs in his study of vertebral developmental errors in Canadian Inuit skeletons, where a higher frequency and intensity of several spine defects were observed in the smaller and more genetically isolated of the two compared populations. While for North American islander prehistoric populations, it has been suggested that the high prevalence of maxillary canine-premolar transposition could be a signal of endogamy. A more direct approach to inbreeding has been presented by Palma and Carini and Alt et al. The former authors observed a high frequency of cervical ribs at the 7thcervical vertebra in an isolated population from Sicily, while the latter observed a high frequency (35.7%) of congenitally missing maxillary lateral incisors in a 9000-year-old late Pre-Pottery Neolithic community in Southern Jordan. After a thorough review of the literature, these authors suggest that this could only be explained by close familial relationships, in this case due to socio-cultural choice of endogamy.
In general, skeletal findings suggesting inbreeding would fit the demographic scenario for early humans, with small size groups, population dispersal and potentially significant levels of intragroup and intrafamily mating resulting in high levels of consanguinity through time . In this respect, and with regard to a very rare anomaly observed in the parietal bones of Xujiayao 11, an early Late Pleistocene fossil from China, Wu et al.  review the unusually high incidence of rare conditions among Pleistocene humans. These authors suggest the possibility that the high frequency of these conditions could reflect small and highly inbred populations during the Pleistocene, and the congenital clefts of the atlas from the Neandertals of El Sidrón would fit this broader scenario for human population dynamics during the Pleistocene. Specifically for Neandertals, as recently summarized , a similar general picture emerges from recent advances in paleogenetics, which posits a long-term small and decreasing population size sometime after 0.5–1.0 million years ago, with reduced and isolated groups. The consequence would be increased inbreeding at times. For instance, genetic analysis from two Neandertal individuals, from El Sidrón and Vindija respectively, indicate that mating among related individuals may have been more common in Neandertals than in present-day humans . This observation would join with genetic evidence from a Neandertal woman from Siberia indicating a close familial relation between her parents (e.g. half-siblings with a common mother), further analysis pointing to frequent mating between closely related individuals in Neandertals from this geographic area . Thus, an additional potential interpretation of the findings of dental and skeletal congenital anomalies at El Sidrón could be a signal of inbreeding in Neandertals from this geographic area. Although this inference would fit the general scenario emerging from skeletal studies  of Pleistocene humans and Neandertal paleogenetics , it is clear that further skeletal findings are needed in order to support this interpretation. In this regard, supernumerary ribs associated to the first lumbar vertebra, a low frequency developmental defect in modern humans, have been documented for two Neandertal individuals, Shanidar 3 and Kebara 2 [109, 110].
It is important to note, however, that inbreeding is not the only possible explanation for a high incidence of congenital conditions, which could also be explained by adverse environmental conditions impacting early pregnancies. For instance, congenital defects such as neural tube defects and orofacial clefts have been associated with socioeconomic status and maternal dietary patterns in epidemiological studies[111–113], while supernumerary ribs constitute a common finding in standard developmental toxicology bioassays . Following both possibilities, it has been suggested that a combination of harsh environmental conditions and inbreeding was the most likely explanation for the high incidence in Late Pleistocene mammoths of cervical ribs, a congenital condition that in humans has been associated with multiple and major congenital abnormalities . Studies of enamel hypoplasiaand dental fluctuating asymmetry indicate that Neandertal populations possibly suffered similar , or greater [118, 119] developmental stress than comparative prehistoric modern human samples.Specific evidence from El Sidrón indicates that all the dental individuals showed enamel hypoplasia, with well-marked defects on the incisors (59%), canines (50%), premolars (58%), and molars (32%) . These data only partially support harsh environmental conditions as an explanatory factor for the presence of congenital conditions since they refer specifically to the period of crown formation, and not to the later period of pregnancy.
We present the first two cases in Neandertals of a congenital posterior and anterior cleft of the arch of the atlas respectively. This observation, together with the previously reported presence of dental anomalies for two individuals from this site, could be interpreted as further evidence of the presence of a group with close genetic relations at El Sidrón, as a possible signal of inbreeding in this Neandertal group, and as an indication of harsh environmental conditions. Previous findings of high incidence of rare conditions among Pleistocene humans, and the general demographic scenario for Neandertals that emerges from paleogenetics would be compatible with inbreeding as an explanation of the presence of these low frequency clefts of the atlas in two of three observable atlases from El Sidrón. Further support or rejection ofthis and the aforementioned interpretations will come from new genetic and skeletal evidence from Neandertal remains.
We thank all the people who worked during the El Sidrón fieldwork seasons. We also thank the students working at the Group of Paleoanthropology MNCN-CSIC. Hugo Cardoso and Susana García granted access to the documented and archaeological subadult skeletons curated at the Museo Nacional de Historia Natural, Lisbon, Portugal. Sofia Wasterlain, Teresa Ferreira and the staff of DRYAS granted access to the Lagos archaeological collection and kindly collaborated with the short staying of LR at Coimbra. Eugenia Cunha granted access to the Universidade de Coimbra facilities. Thanks to Asier Gómez-Olivencia for the kind and interesting discussion about the Neandertal atlas. Thanks to the medical and technical staff from the Service of Radiology, Ruber Hospital, Madrid. The comments of two referees and the academic editor were very useful to the authors.
Conceived and designed the experiments: LR AR. Performed the experiments: LR AR. Analyzed the data: LR AR AE AGT MB RH. Contributed reagents/materials/analysis tools: LR AR AE AG MB RH FP JSG MR. Wrote the paper: LR AR.
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