Intestinal infection with the intracellular parasite Toxoplasma gondii results in the translocation of commensal bacteria to peripheral organs and the development of a T cell response specific to the microbiota. In naïve mice, the recently described RORγt+ group 3 innate lymphoid cell (ILC) population plays a critical role in promoting intestinal barrier function and limiting responses to gut-resident commensal bacteria. Given this role for group 3 ILCs, studies were performed to evaluate whether these cells might influence the immune response to mucosal infection with T. gondii. Phenotypic characterization of RORγt+ ILCs in T. gondii infected mice revealed that this population decreased following challenge but the population that remained expressed costimulatory molecules and IL-22. One factor that influences the maintenance of RORγt+ ILCs is the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, and Ahr-/- mice have a marked defect in the lamina propria group 3 ILC population. When Ahr-/- mice were challenged with T. gondii, they lost more weight than wild type controls. This disease course in Ahr-/- animals was associated with increased T cell responses to Toxoplasma antigen and crude commensal antigen preparations. Together, these data suggest that group 3 ILCs have a role in limiting T cell activation during intestinal infection.
Citation: Wagage S, Harms Pritchard G, Dawson L, Buza EL, Sonnenberg GF, Hunter CA (2015) The Group 3 Innate Lymphoid Cell Defect in Aryl Hydrocarbon Receptor Deficient Mice Is Associated with T Cell Hyperactivation during Intestinal Infection. PLoS ONE 10(5): e0128335. https://doi.org/10.1371/journal.pone.0128335
Academic Editor: Markus M. Heimesaat, Charité, Campus Benjamin Franklin, GERMANY
Received: January 22, 2015; Accepted: April 24, 2015; Published: May 26, 2015
Copyright: © 2015 Wagage et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are within the paper.
Funding: This work was supported ny the NIHR01AI-110201 (http://www.niaid.nih.gov). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The ability of cells of the immune system to sense and react to environmental stimuli plays a crucial role in the maintenance of steady state conditions and in the effective control of infection while limiting immune-mediated damage to the host. Immune cells use a variety of sensors to respond to environmental cues, and the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, provides these cells with one means to detect and respond to environmental signals [1, 2]. The AHR binds to structurally diverse agonists that include endogenous compounds such as certain tryptophan metabolites and synthetic molecules exemplified by 2,3,7,8-tetrachlorodibenzo-p-dioxin . AHR ligands derived from plants can also be obtained through the diet , which makes the intestines a major site of exposure to AHR agonists. Accordingly, signaling through this transcription factor has multiple effects on intestinal immune responses. AHR activity in the intestine is required for the development of isolated lymphoid follicles and cryptopatches, and contributes to the maintenance of intraepithelial lymphocytes and group 3 innate lymphoid cells (ILCs) [4–7]. This RORγt+ ILC population has a critical role in the regulation of intestinal barrier function in naïve mice and IL-22 expression by these cells limits the dissemination of the microbiota to distal sites . Group 3 ILCs are also able to present antigen but because they typically lack the expression of costimulatory molecules, they are thought to promote T cell tolerance to commensal bacteria .
The gastrointestinal microbiota influences normal host physiology as well as the immune response to infection with a variety of pathogenic microorganisms . For example, oral infection with the intracellular parasite Toxoplasma gondii can lead to immune-mediated local tissue damage associated with the translocation of commensal bacteria and the development of a T cell response specific to the microbiota [11, 12]. This infection-induced dissemination of commensal bacteria is controlled by neutrophils, which form structures in the lumen that restrict contact between the microbiota and the intestinal epithelium .
Given the role for group 3 ILCs in limiting responses to commensal bacteria at the steady state, studies were performed to evaluate whether these cells might influence the immune response to mucosal infection with T. gondii. Following infection, there was a marked decrease in the frequency of RORγt+ ILCs, but the population that remained expressed costimulatory molecules and IL-22. In order to study the possible effects of RORγt+ ILCs during infection, Ahr-/- mice, which have a prominent defect in this population, were challenged with T. gondii. Infected Ahr-/- animals lost more weight than wild type controls and exhibited increased T cell responses when stimulated with Toxoplasma antigen and crude commensal antigen preparations. These results suggest that group 3 ILCs have a role in limiting T cell activation during infection.
Materials and Methods
Mice and infections
Ahr-/- mice that had been backcrossed onto a C57/Bl6 background for 21 generations were obtained from Dr. Christopher A. Bradfield (University of Wisconsin School of Medicine and Public Health, Madison, WI). C57/Bl6 mice were purchased from the Jackson Laboratory (Bar Harbor, ME). CBA/J mice were purchased from the National Cancer Institute Animal Production Program (Frederick, MD). All mice were bred and housed in specific pathogen-free facilities at the University of Pennsylvania in accordance with institutional guidelines, and these specific studies were approved by the University of Pennsylvania Institutional Animal Care and Use Committee (Protocol 805432). Mice were euthanized using CO2 in a custom flow metered chamber. For infections, Me49 cysts were harvested from the brains of chronically infected CBA/J mice and experimental animals were orally gavaged with 20 or 100 cysts. Infected mice were monitored daily and at the timepoints assessed in this study these animals did not show adverse effects that necessitated sacrifice or the use of analgesics. For the analysis of parasite burdens, DNA was isolated from tissues using the High Pure PCR Template Preparation kit (Roche, Indianapolis, IN). Parasite DNA levels were determined by RT-PCR as previously described .
Spleens and lymph nodes were dissociated through a 40μm filter. Red blood cells in the spleens were lysed with 0.86% ammonium chloride (Sigma) in sterile water. For cell isolation from the gut, small intestines were cut longitudinally after removing the Peyer’s patches and the contents were washed in RPMI. Epithelial cells were stripped with 5mM EDTA and 1mM DTT in RPMI, and the tissue was digested with 0.16U/mL Liberase TL (Roche) and 0.05% DNAse. The suspension was passed through a 70μm filter, and then passed through a 40μm filter.
Cells were cultured at 1–2.5x106 cells/ml in complete RPMI (10% heat inactivated FCS, 2mM glutamine, 10U/ml penicillin, 10μg/ml streptomycin, 1mM sodium pyruvate, 1% nonessential amino acids, 5x10-5M 2-ME) with 62μg/ml soluble Toxoplasma antigen. Supernatants were collected after 48 hours and cytokines were detected by ELISA. For IL-10 ELISAs, Immulon 4HBX plates (Thermo Fisher Scientific, Waltham, MA) were coated with anti-IL-10 (clone JES5-2A5) (BD Pharmingen San Diego, CA), blocked in 5% FBS in PBS, and loaded with samples. Biotinylated anti-IL-10 (clone JES5-16E3) was used for detection followed by peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories, West Grove, PA), SureBlue (KPL, Gaithersburg, MD) and TMB Stop Solution (KPL).
Stimulation with antigen preparations
Crude commensal antigen preparations were generated as previously described . Briefly, small intestinal contents were collected in PBS and centrifuged at 1000 rpm to eliminate large aggregates. The supernatant containing the majority of bacteria was collected and centrifuged at 8000 rpm to pellet the bacteria. The pellet was resuspended in PBS, sonicated on ice, centrifuged at 20000g for 10 minutes, and the supernatant was used as a source of bacterial antigens. To evaluate T cell IFN-γ production, splenocytes were stimulated with 10μg/mL of the commensal antigen preparations or with 10μg/mL soluble Toxoplasma antigen for 5 hours. Brefeldin A was then added to the cultures overnight. The cells were surface stained, fixed, and stained for IFN-γ.
For intracellular cytokine staining, cells were incubated with brefeldin A (Sigma) and PMA and ionomycin for 4 hours, surface stained, and fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Cells were then permeabilized with 0.5% saponin (Sigma) and stained for cytokines. For surface staining, samples were washed in flow cytometry buffer containing 1% BSA (Sigma) and 2mM EDTA (Invitrogen) in PBS, Fc blocked with 2.4G2 and normal rat IgG (Invitrogen), and stained with monoclonal antibodies. For the detection of T-bet, RORγt, FoxP3, and Ki67, cells were surface stained and then stained intracellularly for these proteins using the FoxP3/transcription factor staining buffer set (eBioscience). The following antibodies were purchased from eBioscience: T-bet eFluor660, CD3 APC-eFluor780, TNF-α FITC, IFN-γ PeCy7, IFN-γ PE, FoxP3 eFluor450, RORγt PE, CD3 PerCP-Cy5.5, CD5 PerCP-Cy5.5, CD11c APC-eFluor780, B220 APC-eFluor780, CD80 FITC, MHC II APC. The following antibodies were purchased from BD Pharmingen: Ki67 FITC, CD4 PE, and CD8 PerCP-Cy5.5. The following antibodies were purchased from BioLegend: CXCR3 PeCy7, NK1.1 PerCP-Cy5.5, CD90.2 AF700, IL-22 Alexa Fluor 647. Live/Dead Fixable Aqua dead cell stain (Life Technologies) was used to discriminate between live and dead cells. Samples were run on a FACSCanto II or LSRFortessa and data was analyzed using Flowjo software (Tree Star, Inc., Ashland, OR).
To evaluate a possible role for group 3 ILCs during T. gondii infection, experiments were performed to assess the phenotype of these cells following oral challenge with this parasite. Group 3 ILCs in naive mice expressed IL-22, and this was not significantly altered during toxoplasmosis (Fig 1A). Since RORγt+ ILCs are able to present antigen , their expression of MHC class II and the costimulatory molecule CD80 was evaluated. An increased frequency of group 3 ILCs expressed MHC class II during toxoplasmosis (Fig 1B). In naïve mice, CD80 was not widely expressed by RORγt+ ILCs, but after infection this costimulatory molecule was expressed by a subset of group 3 ILCs (Fig 1B). Notably, oral infection with T. gondii led to a decrease in the frequency and number of RORγt+ ILCs in the lamina propria (Fig 1C), which was associated with decreased expression of RORγt by these cells in infected mice and increased expression of Ki67 (Fig 1D). These results show that challenge with T. gondii led to marked changes in the group 3 ILC population, and suggest that despite the overall reduction in this population, there was an increased frequency of these cells undergoing proliferation.
Wild type mice were infected orally with 20 Me49 cysts for 9 days, and cells were isolated from the lamina propria of infected mice or naïve controls. Group 3 ILCs were gated as live NK1.1-CD3-CD5-CD11c-B220-CD90.2+RORγt+ cells. Results are pooled from 2–5 separate experiments. (A) IL-22 production by group 3 ILCs following stimulation with PMA/ionomycin. (B) MHC II and CD80 expression by group 3 ILCs. (C) Frequency and number of group 3 ILCs in the lamina propria. The plots on the left are gated on live CD90.2+CD11c-B220- cells. (D) RORγt and Ki67 expression by group 3 ILCs.
The infection-induced changes in the RORγt+ ILC population raised the question of whether these cells influence the immune response to toxoplasmosis. Notably, naïve Ahr-/- mice have a marked reduction in group 3 ILCs in the lamina propria [5–7], and therefore experiments were performed to evaluate the responses of these mice to T. gondii. The defect in the RORγt+ ILC population seen in naïve mice in the absence of the AHR was also apparent in infected Ahr-/- animals (Fig 2A). Following oral challenge, weight loss in wild type and Ahr-/- mice was monitored to determine disease progression, which indicated that infected Ahr-/- mice lost more weight than wild type animals (Fig 2B). Histological evaluation of small intestinal tissue from multiple experiments with a total of 16 wild type and 12 Ahr-/- mice that had been infected for 9 days indicated that all infected animals had lymphoplasmacytic inflammation expanding the lamina propria of the small intestine with areas of enterocyte loss and necrosis. The Peyer’s patches exhibited mild to severe lymphocytolysis (Fig 2C–2F). Grading of histological sections of the small intestine by an anatomical pathologist revealed that wild type mice typically had mild to moderate inflammation whereas Ahr-/- animals had moderate to severe disease, consistent with the increased weight loss observed in these mice.
Wild type or Ahr-/- mice were orally infected with T. gondii for 9 days. (A) Group 3 ILC frequency in the lamina propria of wild type or Ahr-/- mice following infection. The plots on the left are gated on live CD90.2+CD11c-B220- cells. (B) Weight loss was monitored at various days post-infection. Data are pooled from 2 experiments with 6–8 mice per group. (C) H&E staining of small intestinal tissue sections. (D) H&E staining of small intestinal tissue from an infected Ahr-/- mouse. The Peyer’s patch exhibits severe lymphocytolysis (*) and the lamina propria of adjacent villi is expanded by primarily lymphocytes and plasma cells (➜). (E) A higher magnification image of the section in Fig 2C shows that the Peyer’s patch exhibits severe lymphocytolysis characterized by pyknosis and other cellular debris. (F) H&E staining of small intestinal tissue from an infected Ahr-/- mouse shows crypt loss (*) and multifocal necrotic enterocytes (➜). The lamina propria of the villi is expanded by lymphocytes and plasma cells (❋). (G) Expression of T-bet and Ki67 by FoxP3- CD4+ T cells in the mesenteric lymph nodes of infected mice. Results are pooled from 3 separate experiments. (H) Cytokine production by CD4+ T cells following stimulation with PMA/ionomycin. Data are pooled from 2 experiments.
Since the development of a Th1 CD4+ T cell response contributes to immune-mediated pathology during oral toxoplasmosis , studies were performed to assess whether the absence of the AHR influenced T cell responses. In naive wild type and Ahr-/- animals, similar frequencies of CD4+ T cells expressed Ki67 and the Th1 associated transcription factor T-bet (data not shown). However, compared to wild type mice, the CD4+ T cell population in infected Ahr-/- animals expressed higher levels of T-bet and elevated amounts of Ki67 (Fig 2G). Consistent with the increased activation phenotype of Ahr-/- CD4+ T cells during infection, these lymphocytes produced increased amounts of IFN-γ following stimulation with PMA/ionomycin (Fig 2H). Collectively, these results indicated that Ahr-/- mice developed a hyperactivated CD4+ T cell response during toxoplasmosis and were more susceptible to this challenge.
Since AHR signaling can promote Treg cell development [16, 17] and the production of IL-10 [18–21], it was possible that alterations in these pathways would contribute to the increased T cell activation seen in Ahr-/- mice. However, it should be noted that Treg frequencies were similar in infected wild type and Ahr-/- animals (Fig 3A). In addition, Treg expression of T-bet and CXCR3, which promote Treg function by allowing these cells to migrate to sites of inflammation and limit Th1 responses [22, 23], was not impaired in the spleen, mesenteric lymph nodes, or lamina propria of Ahr-/- animals (Fig 3B and data not shown). Similarly, no major defect in IL-10 production following restimulation with Toxoplasma antigen was detected in infected Ahr-/- mice (Fig 3C). These results indicate that the increased CD4+ T cell activation seen in these animals was not mediated by overt defects in the Treg cell population or the production of IL-10.
Ahr-/- mice or wild type controls were infected orally with 20 Me49 cysts for 9 days. Results are pooled from 2–3 separate experiments. (A) Frequency of Tregs in the indicated tissues of wild type or Ahr-/- mice. In the lamina propria, the plots shown are gated on live CD45+CD3+CD4+ cells, and Tregs were gated as live CD45+CD3+CD4+FoxP3+ cells. For the mesenteric lymph node and spleen, the plots shown are gated on CD3+CD4+ cells, and Tregs were gated as CD3+CD4+FoxP3+ cells. (B) Expression of T-bet, CXCR3, and Ki67 on Tregs in the spleens of wild type or Ahr-/- mice. The plots are gated on CD3+CD4+FoxP3+ cells. (C) IL-10 secretion by cells isolated from the lamina propria or the spleen following restimulation with soluble Toxoplasma antigen. Results are pooled from 2 separate experiments with a total of 5–7 mice per group.
Since infected Ahr-/- mice have a marked reduction in group 3 ILCs in the lamina propria and RORγt+ ILCs promote tolerance to the microbiota , experiments were performed to assess whether the hyperactive T cell response in Ahr-/- animals was related to increased responses to commensals during infection. Splenocytes from infected mice were stimulated with parasite antigen or with commensal antigen preparations generated from small intestinal luminal contents, and T cell production of IFN-γ production was assessed. Since the composition of the microbiota changes markedly following infection with T. gondii [13, 24], crude commensal antigen preparations were made from the luminal contents of naïve and infected animals. However, it was possible that the commensal antigen preparation generated from infected animals contained a low level of T. gondii, since the parasite can be found in the intestinal lumen following infection . To evaluate this possibility, mice were immunized intraperitoneally with a replication deficient strain of T. gondii, which would not lead to disruption of the intestinal barrier or the generation of a microbiota-specific T cell response. Splenocytes from these mice were stimulated with different antigen preparations, and IFN-γ production was analyzed. Stimulation with Toxoplasma antigen induced IFN-γ expression by these cells, but stimulation with the commensal antigen preparation from infected mice elicited negligible levels of IFN-γ compared to cells that were unstimulated or treated with commensal antigen from naïve mice (data not shown). These results indicated that the commensal antigen generated from infected mice did not contain sufficient levels of parasite protein to induce a large T. gondii-specific response.
To evaluate the responses to the commensal antigen in wild type and Ahr-/- mice, splenocytes from mice that had been orally infected with the Me49 strain of T. gondii were stimulated. Interestingly, stimulation of splenocytes from infected wild type mice with crude commensal antigen generated from naïve mice induced a low level of IFN-γ production, while stimulation with commensal antigen from infected mice elicited a higher frequency of IFN-γ + CD4+ T cells (Fig 4A and 4B). When splenocytes from Ahr-/- mice were stimulated with commensal antigen from infected mice, there was an increased percentage of CD4+ T cells that produced IFN-γ (Fig 4A and 4B). An increased frequency of CD4+ T cells from Ahr-/- animals also expressed IFN-γ upon stimulation with Toxoplasma antigen (Fig 4A and 4B). At this timepoint, parasite burdens were similar between infected wild type and Ahr-/- animals (Fig 4C). It should be noted that more significant differences in T cell IFN-γ expression from wild type and Ahr-/- mice were observed when infecting with a higher dose of 100 T. gondii cysts (data not shown), consistent with the notion that increased parasite doses induce more severe intestinal damage [26, 27]. Together, these results suggest that increased T cell responses to commensal antigen contributed to the T cell hyperactivation seen in Ahr-/- mice.
Wild type or Ahr-/- mice were orally infected with 100 T. gondii cysts for 7 days. (A, B) Splenocytes were stimulated with the indicated antigen preparations for 5 hours and then incubated overnight with brefeldin A. The cells were stained to assay IFN-γ expression by CD4+ T cells. The graph shows pooled data from 3 independent experiments. (C) Parasite burdens in the terminal ileum were assayed by RT-PCR. Results are pooled from 2 separate experiments.
Oral challenge of wild type mice with T. gondii led to a reduction in the frequency and number of group 3 ILCs in the lamina propria as well as decreased RORγt expression by these cells. Previous adoptive transfer experiments have found that a subset of group 3 ILCs can downregulate RORγt expression and develop the ability to produce IFN-γ, a process that is promoted by IL-12 signals . IL-12 expression during oral toxoplasmosis may induce a similar conversion of RORγt+ ILCs into IFN-γ expressing ILCs, which were recently shown to have a protective role in the context of this infection . Notably, downregulation of RORγt in group 3 ILCs is associated with the proliferation of these cells , and an increased frequency of group 3 ILCs expressed Ki67 following challenge with T. gondii. Therefore, the reduction in the group 3 ILC population during infection may be due a conversion of these cells into IFN-γ producing ILCs. Alternatively, the decrease in the RORγt+ ILC population in spite of its increase in Ki67 expression may be due to an increased level of apoptosis in these cells. Given the role of RORγt+ ILCs in promoting the anatomical containment of commensal bacteria , a reduction in this cell population could contribute to the barrier dysfunction that occurs during toxoplasmosis [11, 12]. Since RORγt+ ILCs promote tolerance to commensal bacteria in naïve mice , decreases in this cell population might facilitate the induction of immune responses to infection. This effect would be analogous to the suggestion that the Treg “crash” seen during acute toxoplasmosis facilitates the emergence of an effective T cell response .
Although the RORγt+ ILC population was markedly reduced during toxoplasmosis, some of the cells that remained at 9 days post-infection expressed MHC II and CD80. The increased frequency of MHC II expressing RORγt+ ILCs seen after challenge may be due to a selective enrichment of this subset of cells, as MHC II is expressed by a sub-population of group 3 ILCs in naïve mice . Alternatively, a recent study found that stimulation with IL-1β or Toll-like receptor (TLR) ligands can promote the expression of costimulatory molecules by RORγt+ ILCs . Since IL-1β is expressed in the intestine following T. gondii infection , and there are TLR ligands produced by the parasite  or by commensal bacteria , it is possible that these signals contribute to the group 3 ILC CD80 expression seen during toxoplasmosis. Regardless, additional studies are needed to understand how CD80 expression impacts RORγt+ ILC function during infection.
Although the AHR can contribute to Treg development  and T cell IL-10 production , processes that constrain the immune response during toxoplasmosis [34, 35], no deficiencies in these pathways were observed in infected Ahr-/- animals. Notably, FoxP3- T-bet+ Th1 cells are thought to be the critical source of IL-10 during toxoplasmosis , but since previous studies have shown that the AHR is not expressed at detectable levels in Th1 cells it was unlikely that AHR activity would directly affect IL-10 production by this population . The group 3 ILC defects seen in Ahr-/- mice raised the possibility that this phenotype could contribute to the increased T cell activation seen in AHR deficient animals following infection. Consistent with a role for these innate cells in limiting microbiota-specific responses during toxoplasmosis, an increased frequency of CD4+ T cells in Ahr-/- mice produced IFN-γ following stimulation with crude commensal antigen preparations. However, it is important to note that the AHR affects multiple aspects of the immune response, and other AHR functions may contribute to the phenotype of Ahr-/- mice during T. gondii infection. For example, AHR activity also affects the function of macrophages and dendritic cells [18, 19].
Since IL-22 has been shown to promote tissue pathology during toxoplasmosis [38–40], it was unexpected that Ahr-/- mice with a marked defect in IL-22-producing RORγt+ ILCs would develop increased intestinal pathology. These phenotypes may be related to differences in the microbiomes of Ahr-/- and IL-22 deficient mice. IL-22 deficient animals exhibit an altered microbiome compared to wild type mice , but the composition of the microbiota in wild type and Ahr-/- mice is comparable . Alternatively, these phenotypic differences may be due to additional effects of the AHR in immunity beyond IL-22 induction. For example, functions of RORγt+ ILCs other than their IL-22 expression, such as antigen presentation, may influence the phenotype of infected Ahr-/- animals.
A previous report in which Ahr-/- mice were infected intraperitoneally with T. gondii found that these mice were more susceptible during chronic infection, which was associated with decreased levels of serum IL-10 and decreased parasite burdens in the brain . In contrast, following oral infection, there were no overt defects in IL-10 expression and wild type and Ahr-/- animals had similar parasite burdens during the acute stage. Together, these results suggest that the AHR has context-dependent effects following different routes of challenge and/or in acute and chronic infection. One potential explanation for these different roles is that relevant sources of AHR ligands are likely to vary in distinct settings and can have disparate immunological effects . In the gut, the ability of dietary compounds to activate the AHR contributes to the maintenance of group 3 ILCs , while other AHR agonists can be produced by commensal bacteria . It is unclear whether the infection-induced perturbations to the gut microbiota that occur during toxoplasmosis influence AHR activity [13, 24], but T. gondii can also produce the AHR agonist lipoxin A4 [44, 45], and the AHR has been implicated in the direct sensing of pathogen-associated molecular patterns . In addition to these exogenous sources of AHR ligands, compounds that activate this transcription factor can be produced as part of the host immune response to the parasite. For example, L-kynurenine, which is generated by tryptophan degradation catalyzed by the IFN-γ induced enzyme indoleamine 2,3 dioxygenase, is found in multiple tissues during toxoplasmosis and acts as an AHR agonist [3, 47]. Thus, sensing through the AHR of environmental, pathogen, or host-derived compounds could potentially contribute to its effects during oral infection with T. gondii.
Conceived and designed the experiments: SW GFS CAH. Performed the experiments: SW GHP LD. Analyzed the data: SW GHP ELB. Wrote the paper: SW CAH.
- 1. Stockinger B, Di Meglio P, Gialitakis M, Duarte JH. The aryl hydrocarbon receptor: multitasking in the immune system. Annual review of immunology. 2014;32:403–32. Epub 2014/03/25. pmid:24655296.
- 2. Wagage S, Hunter CA. Interrelated Roles for the Aryl Hydrocarbon Receptor and Hypoxia Inducible Factor-1α in the Immune Response to Infection. Current Immunology Reviews. 2015:in press.
- 3. Denison MS, Nagy SR. Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemicals. Annual review of pharmacology and toxicology. 2003;43:309–34. Epub 2003/01/24. pmid:12540743.
- 4. Li Y, Innocentin S, Withers DR, Roberts NA, Gallagher AR, Grigorieva EF, et al. Exogenous stimuli maintain intraepithelial lymphocytes via aryl hydrocarbon receptor activation. Cell. 2011;147(3):629–40. Epub 2011/10/18. pmid:21999944.
- 5. Qiu J, Heller JJ, Guo X, Chen ZM, Fish K, Fu YX, et al. The Aryl Hydrocarbon Receptor Regulates Gut Immunity through Modulation of Innate Lymphoid Cells. Immunity. 2012;36(1):92–104. Epub 2011/12/20. pmid:22177117; PubMed Central PMCID: PMC3268875.
- 6. Kiss EA, Vonarbourg C, Kopfmann S, Hobeika E, Finke D, Esser C, et al. Natural aryl hydrocarbon receptor ligands control organogenesis of intestinal lymphoid follicles. Science. 2011;334(6062):1561–5. Epub 2011/10/29. pmid:22033518.
- 7. Lee JS, Cella M, McDonald KG, Garlanda C, Kennedy GD, Nukaya M, et al. AHR drives the development of gut ILC22 cells and postnatal lymphoid tissues via pathways dependent on and independent of Notch. Nature immunology. 2012;13(2):144–51. Epub 2011/11/22. pmid:22101730.
- 8. Sonnenberg GF, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, et al. Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria. Science. 2012;336(6086):1321–5. Epub 2012/06/08. pmid:22674331; PubMed Central PMCID: PMC3659421.
- 9. Hepworth MR, Monticelli LA, Fung TC, Ziegler CG, Grunberg S, Sinha R, et al. Innate lymphoid cells regulate CD4+ T-cell responses to intestinal commensal bacteria. Nature. 2013;498(7452):113–7. Epub 2013/05/24. pmid:23698371; PubMed Central PMCID: PMC3699860.
- 10. Abt MC, Artis D. The dynamic influence of commensal bacteria on the immune response to pathogens. Current opinion in microbiology. 2013;16(1):4–9. Epub 2013/01/22. pmid:23332724; PubMed Central PMCID: PMC3622187.
- 11. Hand TW, Dos Santos LM, Bouladoux N, Molloy MJ, Pagan AJ, Pepper M, et al. Acute gastrointestinal infection induces long-lived microbiota-specific T cell responses. Science. 2012;337(6101):1553–6. Epub 2012/08/28. pmid:22923434; PubMed Central PMCID: PMC3784339.
- 12. Benson A, Pifer R, Behrendt CL, Hooper LV, Yarovinsky F. Gut commensal bacteria direct a protective immune response against Toxoplasma gondii. Cell host & microbe. 2009;6(2):187–96. Epub 2009/08/18. pmid:19683684; PubMed Central PMCID: PMC2746820.
- 13. Molloy MJ, Grainger JR, Bouladoux N, Hand TW, Koo LY, Naik S, et al. Intraluminal containment of commensal outgrowth in the gut during infection-induced dysbiosis. Cell host & microbe. 2013;14(3):318–28. Epub 2013/09/17. pmid:24034617.
- 14. Silver JS, Stumhofer JS, Passos S, Ernst M, Hunter CA. IL-6 mediates the susceptibility of glycoprotein 130 hypermorphs to Toxoplasma gondii. J Immunol. 2011;187(1):350–60. Epub 2011/05/25. pmid:21606248; PubMed Central PMCID: PMC3119722.
- 15. Liesenfeld O, Kosek J, Remington JS, Suzuki Y. Association of CD4+ T cell-dependent, interferon-gamma-mediated necrosis of the small intestine with genetic susceptibility of mice to peroral infection with Toxoplasma gondii. The Journal of experimental medicine. 1996;184(2):597–607. Epub 1996/08/01. pmid:8760813; PubMed Central PMCID: PMC2192709.
- 16. Kimura A, Naka T, Nohara K, Fujii-Kuriyama Y, Kishimoto T. Aryl hydrocarbon receptor regulates Stat1 activation and participates in the development of Th17 cells. Proceedings of the National Academy of Sciences of the United States of America. 2008;105(28):9721–6. Epub 2008/07/09. pmid:18607004; PubMed Central PMCID: PMC2474493.
- 17. Quintana FJ, Basso AS, Iglesias AH, Korn T, Farez MF, Bettelli E, et al. Control of T(reg) and T(H)17 cell differentiation by the aryl hydrocarbon receptor. Nature. 2008;453(7191):65–71. Epub 2008/03/26. pmid:18362915.
- 18. Nguyen NT, Kimura A, Nakahama T, Chinen I, Masuda K, Nohara K, et al. Aryl hydrocarbon receptor negatively regulates dendritic cell immunogenicity via a kynurenine-dependent mechanism. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(46):19961–6. Epub 2010/11/03. pmid:21041655; PubMed Central PMCID: PMC2993339.
- 19. Kimura A, Naka T, Nakahama T, Chinen I, Masuda K, Nohara K, et al. Aryl hydrocarbon receptor in combination with Stat1 regulates LPS-induced inflammatory responses. The Journal of experimental medicine. 2009;206(9):2027–35. Epub 2009/08/26. pmid:19703987; PubMed Central PMCID: PMC2737163.
- 20. Apetoh L, Quintana FJ, Pot C, Joller N, Xiao S, Kumar D, et al. The aryl hydrocarbon receptor interacts with c-Maf to promote the differentiation of type 1 regulatory T cells induced by IL-27. Nature immunology. 2010;11(9):854–61. Epub 2010/08/03. pmid:20676095; PubMed Central PMCID: PMC2940320.
- 21. Wagage S, John B, Krock BL, Hall AO, Randall LM, Karp CL, et al. The aryl hydrocarbon receptor promotes IL-10 production by NK cells. J Immunol. 2014;192(4):1661–70. Epub 2014/01/10. pmid:24403534.
- 22. Koch MA, Tucker-Heard G, Perdue NR, Killebrew JR, Urdahl KB, Campbell DJ. The transcription factor T-bet controls regulatory T cell homeostasis and function during type 1 inflammation. Nature immunology. 2009;10(6):595–602. Epub 2009/05/05. pmid:19412181; PubMed Central PMCID: PMC2712126.
- 23. Hall AO, Beiting DP, Tato C, John B, Oldenhove G, Lombana CG, et al. The cytokines interleukin 27 and interferon-gamma promote distinct Treg cell populations required to limit infection-induced pathology. Immunity. 2012;37(3):511–23. Epub 2012/09/18. pmid:22981537; PubMed Central PMCID: PMC3477519.
- 24. Heimesaat MM, Bereswill S, Fischer A, Fuchs D, Struck D, Niebergall J, et al. Gram-negative bacteria aggravate murine small intestinal Th1-type immunopathology following oral infection with Toxoplasma gondii. J Immunol. 2006;177(12):8785–95. Epub 2006/12/05. pmid:17142781.
- 25. Gregg B, Taylor BC, John B, Tait-Wojno ED, Girgis NM, Miller N, et al. Replication and distribution of Toxoplasma gondii in the small intestine after oral infection with tissue cysts. Infection and immunity. 2013;81(5):1635–43. Epub 2013/03/06. pmid:23460516; PubMed Central PMCID: PMC3647985.
- 26. Dubey JP, Ferreira LR, Martins J, McLeod R. Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortality. Parasitology. 2012;139(1):1–13. Epub 2011/11/15. pmid:22078010; PubMed Central PMCID: PMC3683600.
- 27. Schreiner M, Liesenfeld O. Small intestinal inflammation following oral infection with Toxoplasma gondii does not occur exclusively in C57BL/6 mice: review of 70 reports from the literature. Memorias do Instituto Oswaldo Cruz. 2009;104(2):221–33. Epub 2009/05/12. pmid:19430647.
- 28. Vonarbourg C, Mortha A, Bui VL, Hernandez PP, Kiss EA, Hoyler T, et al. Regulated expression of nuclear receptor RORgammat confers distinct functional fates to NK cell receptor-expressing RORgammat(+) innate lymphocytes. Immunity. 2010;33(5):736–51. Epub 2010/11/26. pmid:21093318; PubMed Central PMCID: PMC3042726.
- 29. Klose CS, Flach M, Mohle L, Rogell L, Hoyler T, Ebert K, et al. Differentiation of type 1 ILCs from a common progenitor to all helper-like innate lymphoid cell lineages. Cell. 2014;157(2):340–56. Epub 2014/04/15. pmid:24725403.
- 30. Benson A, Murray S, Divakar P, Burnaevskiy N, Pifer R, Forman J, et al. Microbial infection-induced expansion of effector T cells overcomes the suppressive effects of regulatory T cells via an IL-2 deprivation mechanism. J Immunol. 2012;188(2):800–10. Epub 2011/12/08. pmid:22147768; PubMed Central PMCID: PMC3253229.
- 31. von Burg N, Chappaz S, Baerenwaldt A, Horvath E, Bose Dasgupta S, Ashok D, et al. Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses. Proceedings of the National Academy of Sciences of the United States of America. 2014;111(35):12835–40. Epub 2014/08/20. pmid:25136120; PubMed Central PMCID: PMC4156721.
- 32. Villeret B, Brault L, Couturier-Maillard A, Robinet P, Vasseur V, Secher T, et al. Blockade of IL-1R signaling diminishes Paneth cell depletion and Toxoplasma gondii induced ileitis in mice. American journal of clinical and experimental immunology. 2013;2(1):107–16. Epub 2013/07/26. pmid:23885328; PubMed Central PMCID: PMC3714202.
- 33. Yarovinsky F, Zhang D, Andersen JF, Bannenberg GL, Serhan CN, Hayden MS, et al. TLR11 activation of dendritic cells by a protozoan profilin-like protein. Science. 2005;308(5728):1626–9. Epub 2005/04/30. pmid:15860593.
- 34. Gazzinelli RT, Wysocka M, Hieny S, Scharton-Kersten T, Cheever A, Kuhn R, et al. In the absence of endogenous IL-10, mice acutely infected with Toxoplasma gondii succumb to a lethal immune response dependent on CD4+ T cells and accompanied by overproduction of IL-12, IFN-gamma and TNF-alpha. J Immunol. 1996;157(2):798–805. Epub 1996/07/15. pmid:8752931.
- 35. Oldenhove G, Bouladoux N, Wohlfert EA, Hall JA, Chou D, Dos Santos L, et al. Decrease of Foxp3+ Treg cell number and acquisition of effector cell phenotype during lethal infection. Immunity. 2009;31(5):772–86. Epub 2009/11/10. pmid:19896394; PubMed Central PMCID: PMC2814877.
- 36. Jankovic D, Kullberg MC, Feng CG, Goldszmid RS, Collazo CM, Wilson M, et al. Conventional T-bet(+)Foxp3(-) Th1 cells are the major source of host-protective regulatory IL-10 during intracellular protozoan infection. The Journal of experimental medicine. 2007;204(2):273–83. Epub 2007/02/07. pmid:17283209; PubMed Central PMCID: PMC2118735.
- 37. Veldhoen M, Hirota K, Westendorf AM, Buer J, Dumoutier L, Renauld JC, et al. The aryl hydrocarbon receptor links TH17-cell-mediated autoimmunity to environmental toxins. Nature. 2008;453(7191):106–9. Epub 2008/03/26. pmid:18362914.
- 38. Munoz M, Heimesaat MM, Danker K, Struck D, Lohmann U, Plickert R, et al. Interleukin (IL)-23 mediates Toxoplasma gondii-induced immunopathology in the gut via matrixmetalloproteinase-2 and IL-22 but independent of IL-17. The Journal of experimental medicine. 2009;206(13):3047–59. Epub 2009/12/10. pmid:19995958; PubMed Central PMCID: PMC2806449.
- 39. Wilson MS, Feng CG, Barber DL, Yarovinsky F, Cheever AW, Sher A, et al. Redundant and pathogenic roles for IL-22 in mycobacterial, protozoan, and helminth infections. J Immunol. 2010;184(8):4378–90. Epub 2010/03/12. pmid:20220096; PubMed Central PMCID: PMC3170015.
- 40. Munoz M, Eidenschenk C, Ota N, Wong K, Lohmann U, Kuhl AA, et al. Interleukin-22 induces interleukin-18 expression from epithelial cells during intestinal infection. Immunity. 2015;42(2):321–31. Epub 2015/02/15. pmid:25680273.
- 41. Zenewicz LA, Yin X, Wang G, Elinav E, Hao L, Zhao L, et al. IL-22 deficiency alters colonic microbiota to be transmissible and colitogenic. J Immunol. 2013;190(10):5306–12. Epub 2013/04/16. pmid:23585682; PubMed Central PMCID: PMC3646987.
- 42. Sanchez Y, Rosado Jde D, Vega L, Elizondo G, Estrada-Muniz E, Saavedra R, et al. The unexpected role for the aryl hydrocarbon receptor on susceptibility to experimental toxoplasmosis. Journal of biomedicine & biotechnology. 2010;2010:505694. Epub 2010/01/30. pmid:20111744; PubMed Central PMCID: PMC2810477.
- 43. Zelante T, Iannitti RG, Cunha C, De Luca A, Giovannini G, Pieraccini G, et al. Tryptophan catabolites from microbiota engage aryl hydrocarbon receptor and balance mucosal reactivity via interleukin-22. Immunity. 2013;39(2):372–85. Epub 2013/08/27. pmid:23973224.
- 44. Bannenberg GL, Aliberti J, Hong S, Sher A, Serhan C. Exogenous pathogen and plant 15-lipoxygenase initiate endogenous lipoxin A4 biosynthesis. The Journal of experimental medicine. 2004;199(4):515–23. Epub 2004/02/19. pmid:14970178; PubMed Central PMCID: PMC2211821.
- 45. Machado FS, Johndrow JE, Esper L, Dias A, Bafica A, Serhan CN, et al. Anti-inflammatory actions of lipoxin A4 and aspirin-triggered lipoxin are SOCS-2 dependent. Nature medicine. 2006;12(3):330–4. Epub 2006/01/18. pmid:16415877.
- 46. Moura-Alves P, Fae K, Houthuys E, Dorhoi A, Kreuchwig A, Furkert J, et al. AhR sensing of bacterial pigments regulates antibacterial defence. Nature. 2014;512(7515):387–92. Epub 2014/08/15. pmid:25119038.
- 47. Silva NM, Rodrigues CV, Santoro MM, Reis LF, Alvarez-Leite JI, Gazzinelli RT. Expression of indoleamine 2,3-dioxygenase, tryptophan degradation, and kynurenine formation during in vivo infection with Toxoplasma gondii: induction by endogenous gamma interferon and requirement of interferon regulatory factor 1. Infection and immunity. 2002;70(2):859–68. Epub 2002/01/18. pmid:11796621; PubMed Central PMCID: PMC127654.