Melan-a melanocytes were submitted to sequential cycles of anchorage impediment in the presence (Mn clones) or not (control clones) of 50 μM Mn(III)TBAP. Almost control clones showed spindle morphology (A) whereas Mn clones senescent-like aspect (B). Cell proliferation was analyzed by MTT assay in control (C) and Mn clones (D). The clonogenic capability of control (E) and Mn clones (F) was evaluated before escape from senescent-like phenotype by plating 200 cells on 60 mm-dishes. After 9 days, the cell number was estimated by measuring the absorbance after lysing the cells stained with Toluidine blue.
Citation: Molognoni F, de Melo FHM, da Silva CT, Jasiulionis MG (2015) Correction: Ras and Rac1, Frequently Mutated in Melanomas, Are Activated by Superoxide Anion, Modulate Dnmt1 Level and Are Causally Related to Melanocyte Malignant Transformation. PLoS ONE 10(4): e0124983. https://doi.org/10.1371/journal.pone.0124983
Published: April 13, 2015
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