Ecological validity implicates in how far clinical assessments refer to real life. Short clinical gait tests up to ten meters and 2- or 6-Minutes Walking Tests (2MWT/6MWT) are used as performance-based outcomes in Multiple Sclerosis (MS) studies and considered as moderately associated with real life mobility.
Persons with MS performed 10mWT, 6MWT including 2MWT and 7 recorded days by accelerometry. Ecological validity was assumed if walking tests represented a typical walking sequence in real-life and correlations with accelerometry parameters were strong.
In this cohort (n=28, medians: age=45, EDSS=3.2, disease duration=9 years), uninterrupted walking of 2 or 6 minutes occurred not frequent in real life (2.61 and 0.35 sequences/day). 10mWT correlated only with slow walking speed quantiles in real life. 2MWT and 6MWT correlated moderately with most real life walking parameters.
Citation: Stellmann JP, Neuhaus A, Götze N, Briken S, Lederer C, Schimpl M, et al. (2015) Ecological Validity of Walking Capacity Tests in Multiple Sclerosis. PLoS ONE 10(4): e0123822. https://doi.org/10.1371/journal.pone.0123822
Academic Editor: Markus Reindl, Medical University of Innsbruck, AUSTRIA
Received: August 28, 2014; Accepted: March 7, 2015; Published: April 16, 2015
Copyright: © 2015 Stellmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: Data are available from the Sylvia Lawry Centre for researchers who meet the criteria for access to confidential data. The SLC data warehouse, in particular the Ian MacDonald database, was built with the need to contractually accept certain restrictions of the data donors, in particular to make sure that no data is transferred to third parties and the origin of the data is removed from the data sets. Requests to access data should be submitted to Dr. Martin Daumer, Director, SLCMSR e.V. - The Human Motion Institute, Hohenlindener Str. 1, 81677 Munich, email@example.com.
Funding: These authors have no support or funding to report.
Competing interests: The authors of this manuscript have the following competing interests: Martin Daumer is an Academic Editor for PeerJ and has invented the "free heel running pad". He is also the Director of the Sylvia Lawry Centre for MS Research. He is one of the two managing directors of Trium Analysis Online GmbH, together with Michael Scholz (50% ownership each). Trium is a manufacturer of CTG monitoring systems. Dr. Daumer has served on the scientific advisory board for the EPOSA study; has received funding for travel from ECTRIMS; serves on the editorial board of MedNous; is co-author with Michael Scholz on patents re: Apparatus for measuring activity (Trium Analysis Online GmbH), method and device for detecting a movement pattern (Trium Analysis Online GmbH), device and method to measure the activity of a person (Trium Analysis Online GmbH), co-Author with Christian Lederer of device and method to determine the fetal heart rate from ultrasound signals (Trium Analysis Online GmbH), author of method and device for detecting drifts, jumps and/or outliers of measurement values, coauthor of patent applications with Michael Scholz of device and method to determine the global alarm state of a patient monitoring system, method of communication of units in a patient monitoring system, and system and method for patient monitoring; serves as a consultant for University of Oxford, Imperial College London, University of Southampton, Charite, Berlin, University of Vienna, Greencoat Ltd, Biopartners, Biogen Idec, Bayer Schering Pharma, Roche, and Novartis; and receives/has received research support from the EU-FP7, BMBF, BWiMi, and Hertie Foundation. Christian Lederer and Anneke Neuhaus were employed at the SLC - The Human Motion Institute when the study was performed. Michaela Schimpl were employed at TRIUM GmBH when the study was performed. Sven Briken and Nele Götze have declared that no competing interests exist. Jan-Patrick Stellmann and Christoph Heesen report study, travel and educational grants from Merck-Serono, Biogen-Idec, Teva- Sanofi-Aventis and Bayer-Schering. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.
Persons with Multiple Sclerosis (pwMS) rate mobility as one of their most important bodily functions. Multiple Sclerosis (MS) affects mobility in about three out of four patients and mobility impairment increases with disease duration.[2,3] The accepted standard of disability measurement in MS, the Extended Disability Status Scale (EDSS) refers to walking ability in the range between 20 and 500 meters. Mobility restriction in MS is known to be associated with annual productivity loss, annual caregiver time and patients quality of life. Physical inactivity might as well explain a higher risk for cardiovascular diseases in MS. Beside MS, several studies have proven an association between mobility and morbidity, such as risk for dementia and cardiovascular diseases. While the importance of mobility is widely accepted, the ideal measurement approach is still under discussion.[8,9]
Up to now, mobility assessment in MS is based mainly on short clinical gait test, i.e. performance tests as the Timed 25 foot walk (T25FW), the 10 m Walking Test (10mWT) and the 2- respectively 6-Minute Walking Test (2MWT/6MWT).[8,10] The use of performance tests is already restricted as they show high day-to-day variability and are influenced by the time of day.[11,12] Further on, it is unknown, how often 2 or 6 minutes of uninterrupted walking occur in real life and if changes in the performance of walking tests are associated with a change in real life mobility of pwMS. However, treatment approvals already rely on these tests and the T25FW as well as the 6MWT have been recommended as patient relevant mobility outcomes.[9,10,13–15] Mobile accelerometry offers the possibility to gain ecologic valid, objective and reliable mobility data from pwMS in their daily life environment.[16–20] Over a period of 7 days, which might be most representative for peoples’ habits, it allows the assessment of gait speed and other parameters as distance, steps or sequences of a defined walking period. Gijbels et al. found a moderate association between clinical gait tests and the amount of steps within 7 days. Motl et al. reported good correlations between total daily movement counts on the vertical axis and standard measurements as EDSS, T25FW, 6MWT and the self-reported walking scale MSWS-12. Overall, short walking tests have shown only limited ecological validity.
To the best of our knowledge, it has not been investigated in depth, if short clinical tests might be ecologic valid for single aspects of real life mobility. We were interested how often per day pwMS perform uninterrupted walking sequences comparable to the clinical gait tests (e.g. 2 or 6 minutes) and if standard tests might represent specific walking speeds e.g. if a 10 m walk might represents short and faster walks in real life.
The study was designed to investigate the length and frequencies of uninterrupted walking sequences during a 7-day frame. In a second step, cross-sectional correlations between gait tests and accelerometry data should clarify which aspects of real-life mobility might be represented by the clinical walking tests. We performed a data-driven explorative analysis of clinical gait tests and real-life walking speed parameters without any adjustment for multiple testing or a validation strategy with a second cohort.
30 consecutive pwMS with an Expanded Disability Status Scale (EDSS) below 7 from the MS outpatient clinic of the UMC Hamburg were recruited for an explorative comparison of 10mWT, 2MWT and 6MWT with mobile accelerometry.[4,23] All participants gave written informed consent and the regional ethical review board (medical association Hamburg “Ethik-Kommission der Ärztekammer Hamburg”, study-ID: PV4405) approved the investigation. Patients were asked to perform the 10mWT and the 6MWT together with clinical scoring (EDSS) and wear the actibelt during the measurement and afterwards for another 7 days. EDSS rating was performed according to the guidelines from neurostatus.net by neurologists with an experience in MS. For the 10mWT patients were asked to walk ‘at fastest but safe speed’ from a static start. Start and stop time was measured with a stopwatch by a single trained research assistant. For the 6MWT, patients were instructed ‘to walk at fastest speed, and to cover as much distance as possible’ according to published guidelines. Subjects performed the test on a 20 m corridor with turning around at each end. After each minute passed, patients were informed about the time left but not encouraged. As described by Gijbels et al. we defined the distance covered after 2 minutes of the 6MWT as distance for the 2MWT distance. All participants were instructed how to use the actibelt. A trained instructor switched the actibelt on, to start the measurement over the next 7 days. Patients were asked to wear the actibelt except from showering, swimming or while sleeping. At the end of the 7 days period, patients switched off the actibelt and sent it back by mail. Two patients were excluded due to corrupted actibelt data preventing a meaningful analysis.
The actibelt is a tri-axial accelerometer with 100Hz sampling frequency; it has 512 megabytes of memory corresponding to 10 days continuous recording and a battery life of 20 days. The accelerometer is placed inside a belt buckle, which the wearer fixes around the waist by an elasticated belt. With this design, the device is discreet and unobtrusive, is located close to the subject’s centre of mass and on the sagittal symmetry plane of the body. It can either be used for long- term monitoring in a free-living environment (‘‘week-in-a-box”) or activity assessment in a clinical setting (‘‘rapid tests”).[18,25]
We performed descriptive statistics of demographic variables for the cohort. We calculated mean walking speeds for the 10mWT and 6MWT (distance/time measured by the investigator).[26,27] Beside accelerometry adherence parameters (time of measurement, time of adherence, proportion of “up-side-down” time) we extracted the frequencies of uninterrupted walking sequences of 6 minutes, 2 minutes, 30 seconds and 15 seconds within one week. Standard gait parameters were extracted from the raw actibelt week measurement: distance per day, number of steps per day and hour, walking speed (mean = 50%-quantile, max, min) and walking speed in sequences with 50 or 100 consecutive steps. We assumed, that different clinical gait tests might be associated with different tasks in real-life mobility. Those tasks might be represented by different walking speeds, e.g. fast walking speeds to reach a ringing phone or slower speeds while taking a longer walk. We extracted for each patient a spectrum of walking speeds in quantiles of 5% steps. 0% indicated the lowest measurable individual walking speed, 50% the mean walking speed and 100% the highest individual measurable walking speed. Real-life walking speeds were calculated based on the most frequent uninterrupted walking sequences. Quantiles of walking speeds could only be calculated if at least ten walking sequences were available. We used the investigator measured 10mWT and 6MWT for analysis.[25,26]
We performed univariate linear regression analyses for the extracted actibelt data with 10mWT walking speed, 2MWT and 6MWT independently and calculated coefficient of determination R² and Pearson correlation coefficient as measures of association between the two included variables. Data were analysed using R software for statistical computing and graphics.
The 28 patients of the cohort represented a typical moderately disabled MS population (median EDSS 3.2). Participants were predominantly female (64%), had a median age of 45 and were mainly in a relapsing-remitting MS (RRMS) disease course. The complete descriptive statistics of the cohort is presented in Table 1. The adherence to the 7-days accelerometry was very good with a median measurement time per day of 11.1 hours (range 6.5, 20.3).
Descriptive statistics of the accelerometry measurement and the clinical gait tests are presented in Table 2. 10mWT walking speed (1.4 m/s) was higher than in the other tests (2MWT: 1.3 m/s; 6MWT: 1.4 m/s).
6-minutes sequences of uninterrupted walking occurred only occasionally in real life (median 0.35 sequences per day). Even sequences of 2 minutes were registered less than 10 times a day (median 2.61 sequences per day) and sequences of 30 seconds 11.3 times per day. (Fig 1) For calculations of real-life walking speed we choose sequences of 15 seconds with a median of 22 sequences per day. Median walking speeds were 1 m/s (range 0.87–1.12) at the 5%-quantile of walking speed, 1.29 m/s (range 1.11–1.60) at the mean-50%-quantile and 1.57 (range 1.25, 2.01) at the 95%-quantile.
Two Patients from the cohort denied completing the 6MWT and another 3 actibelt measurements had to be excluded from correlation analyses as the number of walking sequences was to low to calculate reliable walking speeds. These five patients had an EDSS of 6 or above indicating the need of a walking assistance or maximal walking range of less than 100m. Therefore only patients with an EDSS below 6.0 (n = 23) were available for correlation analyses.
Mean number of uninterrupted walking sequences per day for each patient during a 7-day assessment of real life mobility with a mobile accelerometer. Data presented as boxplots with median, 25%-/75%-quantiles and whiskers representing 5%-/95%-quantiles.
The results of the correlations between 10mWT and accelerometry data are shown in Table 3. Only lower quantiles (10 and 30%) of absolute walking speed, the mean number of steps per hour and walking speed in sequences with at least 50 steps were significantly correlated with the 10mWT walking speed. The best correlation was found for walking speed in sequences with at least 50 steps (R2 = 0.61, p < 0.01). Dotplots for different quantiles are presented in Fig 2. All other significant models had a low to moderate coefficient of determination R² (0.20–0.56) and the linear models explained only a small amount of the variability.
Walking speeds are based on sequences of at least 15 seconds uninterrupted walking, 6MWT = 6 Minute Walking Test, 2MWT = 2 Minute Walking Test, 10mWT = 10 meter Walking Test.
Correlations between 6MWT and accelerometry parameters are included in Table 3. Most accelerometry walking speed parameters were significantly correlated with 6MWT, while mean distance per hour and mean number of steps per hour was not. The correlation of 6MWT with different walking speeds was consistently moderate (R2: 0.47–0.52). (Fig 2) The best correlations were found for sequences with at least 50 or 100 steps (R2 = 0.68 respectively 0.71).
The results of the correlations between 2MWT and accelerometry parameters are as well summarized in Table 3. All but one accelerometry walking speed parameter were significantly correlated with 2MWT. Only mean distance per day was not. 6MWT, 2MWT correlated moderately with all real-life walking speeds. (Fig 2) The best correlation was found for sequences with at least 50 steps (R2 = 0.79). Results of the 2MWT and 6MWT were therefore comparable.
This study explored the ecological validity of standard clinical mobility tests in MS patients with a serial assessment of real-life mobility. Sequences of 6 minutes uninterrupted walking could hardly be detected in a representative mildly disabled MS cohort, implicating that the 6MWT does not represent a typical daily-life walking task. Even sequences of 2 minutes occurred only three times a day. The most frequent uninterrupted walking sequences in our cohort were not much longer than 15 seconds. These findings indicate that even if accelerometry data are assessed in real life the method itself must be studied in detail to guarantee that extracted data are meaningful and ecologic valid. Further investigations need to clarify which accelerometry data show the best association with patient relevant outcomes as quality of life, loss of productivity, need for care and mortality.
We used walking speed as primary outcome from the 7-day measurement as it is known that there is a continuous and robust decline of real life accelerometry gait speed with age and a lower variability compared to distance per day or number of steps. Total movement counts were not included in this study as they are an established measure of physical activity rather than a specific mobility outcome.[29,30] Even if walking abilities contribute to total movement counts, motivational or behavioural factors have a major impact on this measures. Our data set did not allow a distinguished analysis of these factors. For the first time, we analysed precisely the association between different walking speed levels and clinical gait tests. The 10mWT showed only a moderate correlation with lower daily-life walking speeds, while speeds above the 30%-quantile of walking speed were not significantly associated. This contrasts the observation that walking speed during the clinical tests is about 1.4 m/s what represents the 75%-quantile of absolute daily-life walking speeds in our cohort, but are in line with the small analysis (n = 10) in traumatic brain injury patients by Moseley et al. They compared 6MWT and 10m-walks with gait speed assessments in three real life situations (car park, shopping centre and hospital corridor) and observed a higher gait speed in the standard clinical tests. Moseley suggested that these differences might rely on the “closed task” of clinical tests. A clear start and stop or the given time frame might represent a high motivational input to walk faster than usual.
Overall, 2MWT and 6MWT had a better and consistent correlation with all quantiles of real life walking speeds than the 10mWT but still explain only about a half of the variance of daily life walking speed. This moderate association confirms previous findings from Gijbels et al, who found similar correlations for habitual walking steps and 2/6MWT (R2: 0.42 and 0.46) while T25FW was even less predictive than the EDSS (R2: 0.31 and 0.33). The findings from Motl et al. support as well a better correlation of accelerometry data with 6MWT than with T25FW. The high association measured by spearman’s Rho in their study for the 6MWT (Rho: 0.63) with total movement counts might point towards the capability of the 6MWT to be more a measure of physical activity than of pure walking, which is as well closer to its original development as a measure of exercise capacity in cardiac failure.  Our analyses did not reveal major differences between 2MWT and 6MWT. This supports the suggestion, that the 2MWT might be seen as an alternative to the 6MWT.
Due to the low to moderate correlation with real-life walking speed, the ecological validity of all performance based gait tests as generalizable measures for mobility is restricted but seems much better for 2MWT and 6MWT than for 10mWT. A precise estimate of real life walking speed seems a very robust putative outcome as it has been shown, that gait speed decreases with age and is inversely associated with quality of life and health status.[17,23,26,27] This is in line with previous studies that found a moderate but robust association between disability, 6MWT and real-life mean walking speed.[8,9,20,24,27,33] Short clinical tests might be ecologic valid measures of physical activity, while accelerometry represents mobility in daily life. This finding challenges the recommendation for short clinical gait tests like the T25FW as valid mobility outcome in MS.[9,10,34] An extensive analysis of T25FW data from the two dalfampidrine trials revealed that a 20% increase in speed in this test is clinically meaningful based on changes in the MSWS-12 self-reported walking scale. In addition, benchmarks for disability levels, restriction in activities of daily living as well as the need for government support was associated with benchmarks of the T25FW. However, this applies to more disabled patients and may not hold through in other cohorts.
We used mobile accelerometry as reference for ecological valid mobility assessments even though this approach has certain restrictions. Calculation of real-life walking speeds failed in more severely disabled patients. Therefore our findings are not transferable to patients needing a walking aid. Patients are still aware of “being measured”—at least initially—and the belt cannot be worn completely unseen with usual slim fit clothes. Further on, we were not able to compare the frequencies of uninterrupted walking periods with healthy individuals. Although, walking speed has been proven as robust marker for mobility with substantial correlation to strong outcomes such as morbidity or mortality, its relationship with as e.g. complex social behaviour has not been studied in depth.[5,7,36] As a limitation, we did not investigate the association between accelerometry data with self-assessment scales as the MSWS-12. Ideally, serial self-assessment e.g. on a smart phone should be combined with a parallel real-life mobility assessment to gain further insight to the ecologic validity of self-assessment scales in MS. Accelerometry might be used in such a setting as a feedback mechanism to enhance physical activity.
Further studies are needed to determine the value of various potential optimizations of the technology platform using mobile accelerometry. For example, single real-life accelerometry parameters must be evaluated concerning their importance for social function, health and quality of life. However, the mobile accelerometry devices we have used in our study and similar devices allow now for the first time a statistically and theoretically meaningful, efficient and objective estimation of what is happening in real life. Motivational factors, personal background (e.g. profession) or time of year as potential covariates of clinical test performance and mobile accelerometry must be further examined in larger and prospective cohorts and its sensitivity to short and long-term changes needs further investigations. Mobile accelerometry might be promising outcome and monitoring tool in MS studies as well as in other neurological diseases. Chabrol and Harrer have recently highlighted this.
2MWT and 6MWT showed a moderate and similar correlation pattern with real-life walking speeds but uninterrupted 2- or 6-minutes walks are uncommon in real life. The 10mWT correlated only with lower quantiles of real life walking speeds and has poor ecologic validity while 2MWT and 6MWT correlated moderately. Mobile accelerometry offers the opportunity to control and improve the ecological validity of MS mobility outcomes.
Conceived and designed the experiments: JPS AN NG SB CL MS CH MD. Performed the experiments: NG JPS CH. Analyzed the data: JPS CH NG SB MS AN MD CL. Wrote the paper: JPS CH AN SB MD CL.
- 1. Heesen C, Böhm J, Reich C, Kasper J, Goebel M, Gold SM (2008) Patient perception of bodily functions in multiple sclerosis: gait and visual function are the most valuable. Mult Scler 14: 988–991. pmid:18505775
- 2. Hobart J, Riazi A, Lamping D, Fitzpatrick R, Thompson A (2003) Measuring the impact of MS on walking ability: The 12-Item MS Walking Scale (MSWS-12). Neurology 60: 31. pmid:12525714
- 3. Paltamaa J, Sarasoja T, Wikström J, Mälkiä E (2006) Physical functioning in multiple sclerosis: a population-based study in central Finland. J Rehabil Med 38: 339–345. pmid:17067966
- 4. Kurtzke JF (1983) Rating neurologic impairment in multiple sclerosis: An expanded disability status scale (EDSS). Neurology 33: 14–44. pmid:6681888
- 5. Cohen JT (2010) Walking speed and economic outcomes for walking- impaired patients with multiple sclerosis. Expert Rev Pharmacoeconomics Outcomes Res 10: 595–604. pmid:20950074
- 6. Wens I, Dalgas U, Stenager E, Eijnde BO (2013) Risk factors related to cardiovascular diseases and the metabolic syndrome in multiple sclerosis − a systematic review. Mult Scler J 19: 1556–1564. pmid:24048545
- 7. Kaltenbach G, Berthel M, Lonsdorfer J, Vogel T, Brechat P, Lepre P (2009) Health benefits of physical activity in older patients: a review. Int J Clin Pr 63: 303–320.
- 8. Gijbels D, Eijnde BO, Feys P (2011) Comparison of the 2- and 6-minute walk test in multiple sclerosis. Mult Scler 17: 1269–1272. pmid:21642370
- 9. Kieseier BC, Pozzilli C (2012) Assessing walking disability in multiple sclerosis. Mult Scler 18: 914–924. pmid:22740603
- 10. Gijbels D, Dalgas U, Romberg A, de Groot V, Bethoux F, Vaney C, et al. (2012) Which walking capacity tests to use in multiple sclerosis? A multicentre study providing the basis for a core set. Mult Scler 18: 364–371. pmid:21952098
- 11. Albrecht H, Wotzel C, Erasmus LP, Kleinpeter M, Konig N, Pollmann W (2001) Day-to-day variability of maximum walking distance in MS patients can mislead to relevant changes in the Expanded Disability Status Scale (EDSS): average walking speed is a more constant parameter. Mult Scler 7: 105–109. pmid:11424630
- 12. Feys P, Gijbels D, Romberg A, Santoyo C, Gebara B, de Noordhout B, et al. (2012) Effect of time of day on walking capacity and self-reported fatigue in persons with multiple sclerosis: a multi-center trial. Mult Scler 18: 351–357. pmid:21969239
- 13. Coleman C, Sobieraj D, Marinucci L (2012) Minimally important clinical difference of the Timed 25-Foot Walk Test: results from a randomized controlled trial in patients with multiple sclerosis. Curr Med Res Opin 28: 49–56. pmid:22073939
- 14. Goodman AD, Brown TR, Edwards KR, Krupp LB, Schapiro RT, Cohen R, et al. (2010) A phase 3 trial of extended release oral dalfampridine in multiple sclerosis. Ann Neurol 68: 494–502. pmid:20976768
- 15. Goodman AD, Brown TR, Krupp LB, Schapiro RT, Schwid SR, Cohen R, et al. (2009) Sustained-release oral fampridine in multiple sclerosis: a randomised, double-blind, controlled trial. Lancet 373: 732–738. pmid:19249634
- 16. Schimpl M, Moore C, Lederer C, Neuhaus A, Sambrook J, Danesh J, et al. (2011) Association between Walking Speed and Age in Healthy, Free-Living Individuals Using Mobile Accelerometry-A Cross-Sectional Study. PLoS One 6: e23299. pmid:21853107
- 17. Motl RW, Weikert M, Suh Y, Sosnoff JJ, Pula J, Soaz C, et al. (2011) Accuracy of the actibelt accelerometer for measuring walking speed in a controlled environment among persons with multiple sclerosis. Gait Posture: 1–5. pmid:21944877
- 18. Schimpl M, Lederer C, Daumer M (2011) Development and validation of a new method to measure walking speed in free-living environments using the actibelt® platform. PLoS One 6: e23080. pmid:21850254
Schlesinger S, Neuhaus A, Thiele A, Kippnich M, Rashid A, Griewing B, et al. Sind Mobilitätseinschränkungen bei Patienten mit Multipler Sklerose messbar? Klinische Neurophysiologie. 2011. pp. 17–21. https://doi.org/10.1055/s-0031-1271750
- 20. Stellmann J-P, Neuhaus A, Stolle C, Goetze N, Soaz C, Schimpl M, et al. (2011) Physical activity in multiple sclerosis: association of 3D-accelerometry data with subjective and rater-based disease status. Mult Scler 17: S13.
- 21. Gijbels D, Alders G, Van Hoof E, Charlier C, Roelants M, Broekmans T, et al. (2010) Predicting habitual walking performance in multiple sclerosis: relevance of capacity and self-report measures. Mult Scler 16: 618–626. pmid:20207785
- 22. Motl RW, Pilutti L, Sandroff B, Dlugonski D, Sosnoff J, Pula JH (2013) Accelerometry as a measure of walking behavior in multiple sclerosis. Acta Neurol Scand 127: 384–390. pmid:23240822
- 23. Daumer M, Thaler K, Kruis E, Feneberg W, Staude G, Scholz M (2007) Steps towards a miniaturized, robust and autonomous measurement device for the long-term monitoring of patient activity: ActiBelt. Biomed Tech (Berl) 52: 149–155. pmid:17313352
- 24. Goldman MD, Marrie RA, Cohen JA (2008) Evaluation of the six-minute walk in multiple sclerosis subjects and healthy controls. Mult Scler 14: 383–390. pmid:17942508
- 25. Soaz C, Daumer M (2012) A feasibility study for the integration of 3D accelerometry in fall risk assessment. Biomed Tech (Berl) 57: 165–168.
- 26. Fischer JS, Rudick RA, Cutter GR, Reingold SC (1999) The Multiple Sclerosis Functional Composite measure (MSFC): an integrated approach to MS clinical outcome assessment. Mult Scler 5: 244–250. pmid:10467383
- 27. Savci S, Inal-Ince D, Arikan H, Guclu-Gunduz A, Cetisli-Korkmaz N, Armutlu K, et al. (2005) Six-minute walk distance as a measure of functional exercise capacity in multiple sclerosis. Disabil Rehabil 27: 1365–1371. pmid:16372431
R Development Core Team (2010) R: A language and environment for statistical computing.
- 29. Butte NF, Ekelund U, Westerterp KR (2012) Assessing physical activity using wearable monitors: measures of physical activity. Med Sci Sports Exerc 44: S5–S12. pmid:22157774
- 30. Sandroff BM, Riskin BJ, Agiovlasitis S, Motl RW (2014) Accelerometer cut-points derived during over-ground walking in persons with mild, moderate, and severe multiple sclerosis. J Neurol Sci 340: 50–57. pmid:24635890
- 31. Moseley AM, Lanzarone S, Bosman JM, Van Loo MA, De Bie RA, Hassett L (2004) Ecological Validity of Walking Speed Assessment After Traumatic Brain Injury A Pilot Study. Lloydia (Cincinnati) 19: 341–348.
- 32. Guyatt GH, Sullivan MJ, Thompson PJ, Fallen EL, Pugsley SO, Taylor DW et al. (1985) The 6-minute walk: a new measure of exercise capacity in patients with chronic heart failure. Can Med Assoc J 132: 919–923. pmid:3978515
- 33. Wetzel JL, Fry DK, Pfalzer LA (2011) Six-minute walk test for persons with mild or moderate disability from multiple sclerosis: performance and explanatory factors. Physiother Can 63: 166–180. pmid:22379256
- 34. Hobart J, Blight AR, Goodman A, Lynn F, Putzki N (2013) Timed 25-Foot Walk: Direct evidence that improving 20% or greater is clinically meaningful in MS. Neurology.
- 35. Goldman MD, Motl RW, Scagnelli J, Pula JH, Sosnoff JJ, Cadavid D (2013) Clinically meaningful performance benchmarks in MS: Timed 25-Foot Walk and the real world. Neurology 81: 1856–1863. pmid:24174581
- 36. Ekblom-bak E, Ekblom B, Vikström M (2013) The importance of non-exercise physical activity for cardiovascular health and longevity. pmid:24167194
- 37. Chabrol H, Harrer JU (2012) Comment: Mobile technology in neurology. Neurology 78: 323. pmid:22218272