Protein P7 is a component of the cystovirus viral polymerase complex. In the unpackaged procapsid, the protein is situated in close proximity to the viral directed RNA polymerase, P2. Cryo-electron microscopy difference maps from the species ϕ6 procapsid have demonstrated that P7 and P2 likely interact prior to viral RNA packaging. The location of P7 in the post-packaged nucleocapsid (NC) remains unknown. P7 may translocate closer to the five-fold axis of a filled procapsid but this has not been directly visualized. We propose that monoclonal antibodies (Mabs) can be selected that serve as probe- reagents for viral assembly and structure. A set of Mabs have been isolated that recognize and bind to the ϕ6 P7. The antibody set contains five unique Mabs, four of which recognize a linear epitope and one which recognizes a conformational epitope. The four unique Mabs that recognize a linear epitope display restricted utilization of Vκ and VH genes. The restricted genetic range among 4 of the 5 antibodies implies that the antibody repertoire is limited. The limitation could be the consequence of a paucity of exposed antigenic sites on the ϕ6 P7 surface. It is further demonstrated that within ϕ6 nucleocapsids that are primed for early-phase transcription, P7 is partially accessible to the Mabs, indicating that the nucleocapsid shell (protein P8) has undergone partial disassembly exposing the protein’s antigenic sites.
Citation: Alimova A, Wei H, Katz A, Spatz L, Gottlieb P (2015) The ϕ6 Cystovirus Protein P7 Becomes Accessible to Antibodies in the Transcribing Nucleocapsid: A Probe for Viral Structural Elements. PLoS ONE 10(3): e0122160. https://doi.org/10.1371/journal.pone.0122160
Academic Editor: Nicholas J. Mantis, New York State Dept. Health, UNITED STATES
Received: December 29, 2014; Accepted: February 15, 2015; Published: March 23, 2015
Copyright: © 2015 Alimova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All data are contained within the paper.
Funding: Funding provided by National Institute of General Medical Science Grant SC1-GM092781, www.nigms.nih.gov (PG); National Institutes of Health Research Centers in Minority Institutions (NIH/NCRR/RCMI) CCNY/Grant G12-RR03060, www.nih.gov (PG). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The cystoviridae family of viruses, of which ϕ6 was the first discovered species, contain three segments of double stranded RNA. Bacteriophage ϕ6 and its relatives are model systems for virus assembly, genome packaging and dsRNA polymerization. The RNA packaging, replication, transcription mechanism, and overall structure resembles that of reoviruses making the species an excellent model system to study these important pathogens. The initial step in cystoviridae replication is the assembly of a closed and unexpanded, dodecahedral-shaped procapsid (PC). The RNA packaging proceeds in a specific order with the small (2948 bp) viral RNA segment packaged first, followed by the middle (4063 bp) and large (6374 bp) segments [1–3]. Step-wise expansion of the PC accompanies the RNA packaging . Ultimately all three ds-RNA segments are enclosed into a nucleocapsid (NC) surrounded by a lipoprotein envelope to constitute the mature viral particle. The outer layer of the NC is a shell composed of a matrix assembled of protein P8 [5–7] that upon cell penetration facilitates an endocytic plasma membrane penetration and is thought to disassemble during viral entry . The P8 shell is composed of 200 trimers arranged as a T = 13 lattice that partially covers the filled PC [5,9,10].
During genome packaging the PC undergoes significant conformational morphogenesis with the sequential expansion revealing unique binding sites for each of the three viral RNA segments [11,12]. The PC is composed of four proteins, P1, P2, P4, and P7, which are responsible for RNA packaging, transcription, and genome replication [11,13]. Three of the four proteins (P1, P2 and P4) are known to have specific functions in regard to the packaging and replication of viral RNA. The entire PC framework is composed of P1 which has RNA binding activity. The atomic structure of P1 for both ϕ6 and ϕ8 has recently been determined and shown to be a flattened trapezoid in shape that adapts to two conformations, P1A and P1B, that undergo conformational changes when maturing from the unexpanded PC to the RNA packaged NC [14–16]. A hexamer of the nucleotide triphosphorylase, P4, forms the packaging portal responsible for RNA transport into the expanding PC. The viral RNA-directed RNA polymerase (RdRP), P2, is required for the replication of the single stranded RNA to the double-stranded RNA (dsRNA) genome .
P7 is the least characterized of the PC proteins and its precise function still remains undetermined. It is required for efficient PC assembly and transcription , and RNA packaging [18,19]. In ϕ6, P7 has a molecular mass of 17168 Da. The ϕ6 virion can potentially contain 60 copies of P7 (three copies at each of the 20 three-fold symmetry axes); but there is a controversy regarding occupancy in recombinant PC particles: SunBamford and Poranen  noted that the same amount of P7 is in recombinant PC particles as in the complete virion. Our previous publication described approximately 20 copies of P7 protein per PC particle ; while NemecekQiaoMindichSteven and Heymann  observed even less occupancy for P7, at only 12 copies in a complete PC. The occupancy of P7 in mature viruses has not been determined and may differ from recombinant PC particles. There is evidence that P7 forms an elongated dimer in solution , but in both the PC and NC, P7 is seen to exist as a monomer. PoranenButcherSimonovLaurinmaki and Bamford  observed that an excess concentration of P7 accelerated assembly of P1 in vitro, indicating that P7 may stabilize P1. Study of self-assembly of PC particles shows potential competition between P2 and P7 proteins . In the presence of an excess of P7, the number of P2 copies decreases from 12 to 6 copies, whereas the number of P7 copies increases from 36 to 60 copies per particle . In prior work, we proposed that P7 is located “inside” the unexpanded PC core near the three-fold axes and that P7 stabilizes P2 in position close to the three-fold axis .
The structure of P7 from the related cystovirus ϕ12 has been determined by X-ray crystallography . Most interestingly, the protein is seen to be composed of two distinct domains in which the N-terminal core region (1–129) of P7 forms a homodimeric α/β-fold, having structural similarities with breast cancer gene 1 (BRCA1) C-terminal domains implicated in multiple protein–protein interactions in DNA repair proteins. The C-terminal tail consisting of approximately 30 to 40 amino acids is less ordered structurally and nuclear magnetic resonance analysis strongly suggests that it is capable of interacting with the viral RNA and the RdRP. Recently, difference maps generated from single particle reconstructions show that in ϕ6, P7 is located very near the inner three-fold axis of symmetry in the unpackaged and unexpanded PC . In that work, it was noted that P7 interacts with P2, and it is proposed that the protein stabilizes the location of P2 near the inner three-fold axis.
Juuti and Bamford  noted that P7 is accessible to polyclonal antibodies from the NC surface and that protein P8 is released by the polyclonal antibodies. While the NC structure is well-established [5,10], actively transcribing NCs have not been directly observed and the precise position and translocation of the RNA portal protein elements have not been determined. In this paper, we report the isolation of a set of monoclonal antibodies (Mabs) with specificity to unique epitopes of P7. These Mabs are utilized as reagents for a first determination of P7 accessibility in a transcribing NC structure. We note that under conditions that favor early-phase ϕ6 transcription, P7 is accessible to antibodies in situ. The Mabs show potential as reagents to reveal viral structure during assembly and disassembly.
Materials and Methods
Cloning, expression, and purification of P7
The ϕ6 P7 coding region was polymerase chain reaction (PCR) amplified from a pLM687 plasmid template  using oligonucleotides OL161 5’-TACTGACAATCCTGTCGTTA-3’ and OL162 5’-TCATCGACATCGAACGGTTG-3’ as upstream and downstream primers, respectively. The PCR product was initially inserted into PCR2.1 TOPO and the recombinant plasmid, designated pPG126, was double digested with restriction endonucleases Ndel and EcoRI. A 498 bp fragment was isolated and ligated with pET28-a vector (Novagen, Madison, WI) at the Ndel and EcoRI sites. The ligation product was transformed in E. coli XL-1 blue supercompetent cells (Novagen, Madison, WI) and selected for kanamycin resistance (50 μg/ml). The positive clone selected was designated pPG128 and was subsequently transformed into T7 Express lysY/Iq Competent E. coli (New England Biolabs, Ipswich, MA). Five ml of overnight culture of the transformants were inoculated in 250 ml Luria-Bertani liquid media, supplemented with 50 μg/ml of kanamycin, and grown at 27°C for 14 h. P7 protein expression was then induced by adding 1 mM of isopropyl-thio-galactoside (IPTG) and the cells were grown at 27°C for an additional 4 h. The expressed protein was visualized by using 12% SDS-PAGE gel of the cell’s samples before and after induction. Induced cells were pelleted by centrifugation at 7000g for 30 min at 4°C and the pellets were stored at −80°C.
The frozen pellets were thawed on ice and re-suspended in 5 ml of lysis buffer consisting of 50 mM of NaH2PO4, 500 mM of NaCl, 1 mM of MgCl2, 10 mM of imidazole and protease inhibitor cocktail (Roche, Nutley, NJ). The cells were sonicated and lysed on ice by using a Microtip Misonix S-4000 sonicator (Qsonica LLC Newtown, CT), at 50% power (13 W) for 10 min, with a repeating power cycle of 10 s ON, 10 s OFF. In order to remove cells debris, the sonicated samples were centrifuged at 20000g for 30 min. The supernatant containing soluble P7 was mixed with Ni-NTA His-Bind resin (Novagen, Madison, WI) pre-equilibrated with washing buffer (50 mM of NaH2PO4, 540 mM of NaCl, 1 mM of MgCl2, 1% glycerol, 20 mM of Imidazole, pH 8.0) and incubated for 2 h in a shaker at room temperature. A gravity flow column was packed with beads incubated with the cell’s supernatant and washed with 50 ml of washing buffer. P7 was eluted with 3 ml of elution buffer (50 mM of NaH2PO4, 540 mM of NaCl, 1 mM of MgCl2, 1% glycerol, 250 mM of imidazol, pH 8.0) and the sample was desalted and concentrated by using Amicron (Millipore, Billerica, MA) 10 kDa cutoff filter.
The P7 concentration was estimated from the density of the band on a Coomassie-stained SDS-PAGE gel. The density data were compared with known amounts of lysozyme protein (MW 14.3 kDa) loaded on the same gel. The amount of protein was estimated by integrating the area under the band curve and applying a background correction.
The growth and purification of ϕ6 followed the procedure described by Mindich et al. . The viral envelope was removed with 1% Triton X-100 to yield isolated NCs by the procedure similar to the one outlined by de HaasPaateroMindichBamford and Fuller . Analysis of virion size (NC diameter is ~58 nm; complete virion diameter is ~86 nm) in TEM micrographs indicated that this procedure resulted in near total removal of the viral envelope.
Four BALB/c female mice were each immunized with 50 μg of P7 in complete Freund’s adjuvant (CFA) on day 0. The mice were boosted on days 21 and 51 with 25 μg and 50 μg of native P7 in incomplete Freund’s adjuvant (IFA) respectively. The mice were bled immediately before each immunization and the sera samples were tested for anti-P7 antibodies by ELISA. One week after the first immunization, the mice were bled and the sera samples were tested for the presence of anti-P7 IgM antibody. The mice were then bled four days after the 1st boost and tested for the presence of anti-P7 IgG antibody. The second boost was performed four days before fusion. Spleen cells from immunized mice were fused with NSO myeloma cell (Sigma Aldrich, St. Louis, MO) using polyethylene glycol 4000 and the hybridoma cells were grown in hypoxanthine aminopteran thymidine (HAT) selection medium.
Hybridomas secreting antibodies that were positive by ELISA for P7 antigen were subcloned by limiting dilution in complete HAT-DME (Dulbecco’s Modified Eagles) medium supplemented with 10% fetal bovine serum, 1% glutamine, 10% NCTC-109 (Life Technologies, Grand Island, NY), 1% of MEM nonessential amino acids (Corning cellgro, Manassas, VA), and 1% penicillin-streptomycin. Hybridoma Cloning Factor (Thermo Fisher Scientific, Waltham, MA) was used to support cell growth. Only subclones, whose secreted antibodies bound P7 but were non-reactive to BSA and a 6-histidine tagged EBNA-1 protein were selected for future propagation. The His-tag EBNA protein was used as a negative control for 6x-his antigen. Initially five selected subcloned hybridomas were grown in Serum-Free DME medium (Sigma-Aldrich, St Louis, MO) to obtain a total of 500 ml of supernatants. All the supernatants were tested by ELISA for anti-P7 IgG antibody and the Mabs labeled as 6C3, 2D11, 1F11, 1B6 and 1C10. A germline analysis search demonstrates that 2D11 is similar to 1B6; 1C10 is similar to 1F11; and therefore, could be derived from the same myeloma fusion (Table 1). In Table 1, the germline gene with the highest alignment is recorded.
Anti-P7 IgG Mabs were purified from the supernatants by using Gamma Bind Plus Sepharose (GE Healthcare Life Sciences, Pittsburgh, PA). The antibody was eluted with 8 ml of 0.1 M glycine at pH 3.0 and immediately adjusted to neutral pH with 800 μl of 1 M Tris-HCl at pH 8.0. The antibody was de-salted and concentrated by an Amicron 10 kDa cut-off filter. Antibody concentration was determined from optical density with a NanoDrop spectrophotometer (NanoDrop division of ThermoScientific, Wilmington, DE).
The IACUC of The City College of New York/CUNY Medical School approved this study—Approval Number 832.2. Mice were anesthetized with isofluorane prior to blood drawing and the mice are euthanized by deep isofluorane inhalation followed by cervical dislocation or CO2 asphyxiation.
The Mabs were isotyped by using an indirect ELISA method. Serial dilutions of the Mabs, starting from an initial concentration of 500 ng/ml were added to wells, and plates were incubated for 1 h at 37°C. The isotype specificity of the Mabs was detected by incubating coated plates with the following goat anti-mouse antibodies conjugated to alkaline phosphatase: anti-IgG1, anti-IgG2a, anti-IgG2b, anti-IgG3 anti-kappa and anti-lambda for 1 h at 37°C. Phosphatase Substrate (Sigma-Aldrich) was added and color development was measured using a Titertek ELISA reader at 405 nm.
Total RNA from 100% confluent T25 flasks were extracted for all four hybridoma cultures by using the TRIzol method, iso-propanol concentrated, and air-dried. The RNA was resuspended in 50 μl of Diethylpyrocarbonate (DEPC) water. The first strand of cDNA was synthesized from the RNA template by using the SuperScript III System for RT-PCR (Invitrogen, Thermo Fisher Scientific, Waltham, MA). Briefly, the 2 μl of total RNA isolated from T25 flasks was reverse transcribed in a reaction volume of 20 μl containing 1 mM of oligo(dT)20, 10 nmol of each of four dNTPs, 40 U of RNaseOUT, 1x RT buffer, 100 nmol of MgCl2, 100 U of SuperScript III reverse transcriptase and 0.2 nmol of dithiothreitol (DTT). Reverse transcription reactions were performed at 50°C for 50 min followed by 85°C for 5 min. Then 1 μl of RNase H was added and samples were incubated at 37°C for 20 min.
Immunoglobulin VH and Vκ transcripts were amplified independently by PCR (High-Fidelity Phusion PCR kit by Finnzymes, Fisher Scientific, Pittsburgh, PA) using 2 μl of RT reaction product as a template. Universal VH forward and reverse γ1 primers and universal forward Vκ and reverse κ primers. were designed by TillerBusse and Wardemann . All PCRs were performed in 50 μl total reaction volume at 94°C for 15 s followed by 50 cycles at 94°C for 30 s; 60°C (VH) or 50°C (Vκ) for 30 s; 72°C for 45 s (VH) or 55 s (Vκ); and a final extension at 72°C for 10 min. Amplified PCR products were gel purified by using QIAquick Gel Extraction Kit (Qiagen, Valencia, CA). Purified products were sequenced (Macrogen Service, Rockville, MD).
For the western blot analysis, 0.3 μg of purified P7 was loaded on to a 12% SDS-PAGE gel. Identical loadings were performed for each of the five Mabs. After gel separation, the proteins were transferred onto a nitrocellulose transfer membrane (BA-S 85 by OPTITRAN) and stained with 0.1% Ponceau S (Sigma-Aldrich, St. Louis, MO) in 5% glacial acetic acid to visualize the transferred bands. The membranes were then cut into five sections for each of the Mabs. The membranes were blocked in 5% skim milk and 0.01% Tween20 in PBS, and then incubated overnight in the presence of 1 μg/ml of each Mab in PBS and 0.01% Tween20. The membranes were then incubated in the presence of a 1:20000 dilution of goat anti-mouse IgG conjugated with HRP (horse radish peroxidase) added to 3% skim milk and 0.01% Tween20 in PBS. To develop the Western Blot Marker (Magic Mark XP Western Standard by Invitrogen) the 1 μl of Precision Protein StrepTactin-HRP conjugated (Bio-Rad Life Sciences, Hercules, CA) was added to each of 10 ml of secondary antibody solution. The membranes were incubated in Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, Rockford, IL) and then Kodak X-OMAT LS film was exposed to visualize the HRP luminescence.
ELISAs to determine whether two Mabs compete for the same or different epitopes on P7
To determine whether two Mabs bound to the same or different epitopes on P7, a mixture of two anti-P7 Mabs were applied to P7 coated ELISA plates. The ELISA plates were coated with 25 ng/ml (1.25 ng per well of native P7). The optimal concentration of P7 antigen per well was obtained by titrating P7 to determine the linear range of activity. One of the Mabs was kept at a constant concentration of 1000 ng/ml (saturation concentration) while the concentration of the other competing Mab was varied in the range of 3 ng/ml to 1000 ng/ml. As a control, a non-competing ELISA was performed for each of the five Mabs in a range of concentrations from 3 ng/ml to 1000 ng/ml. Secondary anti-mouse IgG antibodies conjugated to alkaline phosphatase were added followed by substrate solution.
6C3 Mab biotinylation
The 6C3 Mab was biotinylated by the ChromaLink Biotin One-shot antibody labeling kit, product of Solulink Inc. (San Diego, CA, USA). The biotinylation process was monitored by measuring changes in the optical absorption of the reaction mixture. The calculated biotin molar substitution ratio is 8.35, indicating efficient binding of 6C3. A total of 86 μg of antibody was recovered after the reaction.
The transcription reaction was initiated by incubating 200 μg of NC in 200 μl of transcription reaction mixture [pH 8.8; 70 mM of bicine; 1 mM of Mg Acetate; 3 mM of MnCl2; 125 mM of NH4Cl; 20 mM of DTT; 0.25 U/L of RNasin; approximately 100 μg of macaloid (bentonite clay); and 4 mM of each of the rNTPs] for 1 h at 20°C . As a control, the same amount of NC was utilized in the transcription reaction mixture but without the addition of rNTPs. For each sample, 45 μL was used for RNA transcript extraction. Samples were electrophoresed in 1% agarose gels and stained with ethidium bromide. The cryo-TEM visualization of transcribing NCs used 5 μl each of the control and reaction samples. Electron micrographs were acquired with a JEOL 2100 electron microscope (JEOL Inc. Peabody, MA, USA) operating at 200 keV and a Gatan Orius 2048 × 2048 pixel CCD (Gatan Inc., Pleasanton, CA, USA).
Sandwich ELISA of transcribing NCs
Two Mabs with different epitope recognition, 1C10 and 6C3, were chosen for the sandwich-based ELISA of the NC. The capture antibody was 1C10 and the detecting antibody was biotinylated 6C3. ELISA plates were coated with 5 μg/ml of 1C10 (the Mabs were dissolved in 0.2 M of carbonate-bicarbonate buffer at pH 9.4). The plates were then blocked with 1% BSA in PBS. The transcription reaction mixture (50 μl) was applied to the first well and subsequent 2-fold dilutions with PBS were applied to the remaining wells. Two control samples consisted of: (1) NCs in 100 mM Tris buffer and (2) NCs in transcription buffer but lacking rNTPs were employed for comparison. Following the addition of biotinylated 6C3, plates were incubated with a 1:1000 dilution of streptavidin conjugated with alkaline phosphatase. Substrate solution was added and plates were read at 405 nm using a Titertek Multiscan ELISA reader (TiterTek, Berthold, Pforzheim, Germany).
Results and Discussion
Isolation of ϕ6 P7, inoculation of mice, and genetic classification of the monoclonal antibodies
The SDS-PAGE gel taken at different steps of the P7 purification procedure is shown in Fig. 1 and demonstrates that recombinant his-tagged protein P7 is produced to a high percentage in E. coli in a soluble state. The protein was bound to Ni-NTA beads efficiently eluted with 250 mM of imidazole. Purity was estimated from the coomassie-stained SDS-PAGE and the P7 protein was observed to migrate at a molecular mass of approximately 17 kDa. 50 μg of native form P7 in CFA was used to intraperitoneally inoculate 6-week-old female, BALB/c mice. Two booster inoculations which included P7 emulsified inIFA were administered at days 21 and 51.
Lanes are: Marker (wide range protein ladder p7708); Control—undisrupted cells after IPTG induction; SN—supernatant after the pelleting of lysed cell’s debris; Pellet—resuspended cell debris pellet; Flow-Through—proteins unbound to Ni-NTA beads; 1st and 2nd Wash—proteins eluted from the Ni-NTA agarose column after 1st and 2nd rinses with washing buffer; Eluate—P7 protein eluted from the column.
The Mabs were selected, purified, and their reactivity with P7 investigated. Fig. 2 shows a 12% SDS-PAGE gel of the Mabs revealing the heavy and light chains of the purified IgG monoclonals. The heavy chain molecular weights are 52 KDa for 6C3; and 49 KDa for 2D11, 1B6, 1F11 and 1C10. The light chain molecular weights are 28 KDa for 6C3; and 23 KDa for 2D11, 1B6, 1F11 and 1C10. All five antibodies utilize the IgG1 subclass and the κ light chain as determined by ELISA. The variable region cDNA sequences derived from mRNA of the hybridomas were analyzed by IgBLAST and classification comparisons were made to the mouse database . Sequence analysis reveals that Mabs 1C10, 1B6, 2D11, and 1F11 all utilize a Vκ derived from the same germline gene, Vκ4-59*01 and a VH gene derived from the VH5 family, either the VH5-9-3*01 or VH5-17*02 genes (Table 1). A germline analysis search demonstrates that 2D11 is similar to 1B6, and 1C10 is similar to 1F11 and therefore could be derived from the same myeloma fusion. In Table 1, the germline gene with the highest alignment is recorded. The restricted utilization of Vκ genes and VH genes among 1F11, 1B6, 1C10, and 2D11 suggests that P7 elicits a limited antibody repertoire. We acknowledge that the mutational differences between the similar Mabs could be PCR artifacts, however, the lower binding affinities of 1B6 compared to 2D11, and 1F11 compared to 1C10, suggests that they are independent clones.
MW of the heavy chains are 52 kDa for 6C3; and 49 kDa for 2D11, 1F11 and 1C10. MW of the light chains are 28 kDa for 6C3; and 23 kDa for 2D11, 1F11 and 1C10.
Mab 6C3 differs significantly from the other Mabs and utilizes the VK8–21*01 gene and the V2-9-1*01 heavy chain gene. Query of the IMGT gene database  for Mus musculus indicated that a significant number of antibodies utilizing the VK8-21*01 light chain variable region gene and the V2-9-1*01 gene have specificity for dsDNA. Antibody 6C3 most likely binds a strictly conformational epitope on P7 (see below) that could structurally mimic double stranded nucleic acid polymers thereby eliciting cross-reactivity with dsDNA or dsRNA. Antibody to another viral protein, EBNA-1 has previously been shown to cross-react with dsDNA [31,32]. However 6C3 displayed no cross-reactivity to either dsRNA or dsDNA in nucleic- acid based ELISA tests. It should be noted as well that a search of the database for antibodies utilizing the Vκ4-59*01, or the VH5-9-2*01 or VH5-17*02 genes showed virtually no anti-DNA specificity.
P7 binding characteristics of the monoclonal antibodies
Fig. 3 shows the calibration curve for the binding affinity of each Mab to the native P7 antigen. The ELISA plates were coated with P7 native protein with concentrations ranging from 5 ng/ml to 1000 ng/ml (0.25 ng to 50 ng of P7 per ELISA well). The coating was performed at 4°C overnight to limit the rate of protein denaturation. We first performed preliminary assays to determine the optimal Mab concentration that produced a maximum optical response with the lowest background signal and found that concentration to be 1 μg/ml. Two distinct groups of antibodies, based on antigen affinity, were isolated which had the highest dynamic range for optical absorption. The groups were classified based upon the P7 antigen ELISA response with the following range of linear increase: 1) 20 ng/ml to 100 ng/ml for 6C3 antibody; and 2) 10 ng/ml to 50 ng/ml for the other four Mabs (Fig. 3).
The background absorption for each Mab was determined from the optical density of ELISA plate wells lacking the P7 antigen, and the background optical density was subtracted from the absorption data.
In order to categorize the epitopes as potentially linear or conformational for anti-P7 antibody, equal amounts of either native or denatured proteins were applied to ELISA plate wells. Each experimental group was performed in triplicate. Fig. 4 shows the optical density at 405 nm for an ELISA performed using both native and denatured P7 antigens. No significant difference in the reactivity of 1C10, 2D11, and 1F11 Mabs to native and denatured forms of P7 were observed. This suggests that these Mabs recognize linear epitopes of the P7 antigen. Significant differences in the reactivity of 6C3 and 1B6 between native and denatured P7 indicates that they likely recognizes a conformational epitope. Additional proof of this hypothesis is noted by the result of the Western Blot analysis in that the P7 band is visualized after 1C10, 2D11 and 1F11 labeling, but weak with 1B6, and almost no affinity with 6C3 labeling (Fig. 4B).
A) Comparison of the reactivity of the Mabs 1C10, 6C3, 2D11 and 1F11 to the native and denatured forms of P7. P7 was coated at a concentration of 31.2 ng/ml (1.6 ng per ELISA plate well). Optical density is measured at 405 nm. B) Western Blot of P7 protein.
A competitive ELISA analysis was performed to determine if the Mabs competed for the same epitopes on P7. In the assays, 1.25 ng of native P7 was placed into each of four sets of wells of an ELISA plate. One μg/ml of each Mab was applied to each set of ELISA plate wells. Increasing concentrations of the other three competitor Mabs, were applied separately to each of the ELISA sets. Since all the Mabs are of the γ1 isotype, we cannot directly measure inhibition. Therefore if the saturating and the competitor antibodies are binding the same epitope, we would expect no change in net γ1 antibody binding to P7 even at very high concentrations of the competitor. However, if the saturating and the competitor antibodies are not competing for the same site and are binding different epitopes on P7, then one would expect to see an increase in γ1 antibody binding to P7 with increasing concentration of the competitor. As a control, increasing concentrations of individual Mabs were applied to sets of plates. The control absorption data is presented in Fig. 5A. Optical absorption for wells with saturating Mabs are shown in panels 5B through 5E (B: 6C3; C: 1C10; D: 2D11; E: 1F11). It is evident from Fig. 5B that the reactivity of 6C3 increases regardless of the competitor antibody, indicating a lack of competition between 6C3 and the other three Mabs. For each of the other three sets of wells containing saturating Mabs concentrations, absorption only increases for increased 6C3 concentration—consistent with the results presented in panel B. For none of the other combinations of Mabs does the absorption increase indicating that 1C10, 2D11 and 1F11 are all competing for the same sites on P7. Interestingly, the 6C3 reactivity is not additive with the other three antibodies as evidenced by the fact that optical densities of the mixtures of 6C3 with the other three Mabs is greater than the sum of optical densities of the individual reactions (Fig. 5A). This suggests there could be a synergistic cooperation between 6C3 and each of the other Mabs. It is conceivable that the antigen is structurally altered after binding with the initially added antibody better revealing the second epitope.
Transcribing nucleocapsids can be captured in ELISA
The standard in vitro NC transcription reaction described by EmoriIba and Okada  was established to resemble the early-stage pattern of in vivo viral RNA synthesis in which greater proportion of l transcript is synthesized. The standard reaction is defined by its strict requirement for cations, pH, and l synthesis was found to be exceptionally sensitive to Mn2+ concentration. In addition the early stage of transcription could be associated with a limited or partially assembled P8 matrix. We suggest that the partial disassembly of the NC could occur in the standard reaction and if so the P7 would be in an exposed position. The monoclonal antibodies are ideal reagents for the capture of NC particles primed for transcription assuming the P7 is no longer shielded in the inner 3 fold axis of the procapsid  but has translocated to an open NC position post-RNA packaging.
In order to determine if the Mabs were able to bind to the P7 protein in situ, we performed a sandwich—based ELISA. We chose as a capture antibodies 1C10, 2D11, and 1F11. Antibody 6C3—that recognizes a P7 epitope distinct from 1C10, 2D11, and 1F11- was biotinylated to serve as the detector. The ELISA plate wells were coated with capture antibody at a 5 ug/ml concentration. 50 ul of the NC early phase transcription reaction was applied to the first well and serial 2-fold dilutions in PBS of the reaction mixture were applied to the remaining wells in the ELISA plate row. Two control samples consisted of (1) NC in 100 mM Tris buffer (pH 7.5) and (2) NC in early phase transcription buffer but lacking rNTPs. Following the addition of the biotinylated 6C3 the plates were incubated with a 1:1000 streptavidin conjugated alkaline phosphatase. The substrate solution was added and the degree of NC capture was determined at 405 nm with a Titertek Multiscan ELISA reader. Fig. 6 shows the binding of the Mabs to ϕ6 NC samples after the transcription reaction and that only NCs that were placed into the early phase reaction were captured in the ELISA wells and detected with antibody 6C3. The capture was achieved in early phase buffer in the presence of absence of rNTPs indicating that the NC partial disassembly was nucleotide independent.
Absorption is measured at 405 nm.
We confirmed the partial disassembly of the NC using cryo-EM imaging of early phase transcription reaction mixtures. Cryo-EM projection images of the control (Fig. 7A) shows a large number of intact NCs which appear as 58 nm diameter particles with a high electron density due to the large contribution from the P8 matrix. A corresponding cryo-EM projection image of transcribing NCs (Fig. 7B) reveals that approximately 1/3 of the particles are in an intermediate state in which the P8 matrix is disassembled. NC transcription results in a virion appearing as an expanded hexagonal-shaped PC structure with a reduced internal electron density. Several of these transcribing particles are indicated by black arrows in Fig. 7B.
A) Control sample (no rNTP in transcription reaction mixture); B) NC in 4 mM of rNTP in transcription reaction mixture. Arrows in (B) indicate some of the transcribing NCs (hexagonal configuration with a lower electron density).
We note that the transcribing NC particles and controls (that are primed for transcription in the standard reaction conditions but lacking rNTPs) showed the increasing amount of available P7 in the ELISA reaction. The NCs maintained and tested in 100 mM Tris-HCl buffer (pH 8.0) did not have any evident immunolabeling.
The results of both the projection EM and sandwich assay analysis indicate that the P8 matrix is destabilized and likely completely or partially disassembled under in vitro transcribing conditions- either with or without 4mM rNTPs. The precise position of the transcribing viral RNA within the partially disassembled NC is not evident and its transit path through the portal remains to be determined. Notably the addition of Mabs 6C3 or 1C10 to NC transcribing reactions had no effect on synthesis of viral RNA transcripts.
We conclude the following from the assays: (1) monoclonal antibodies can be elicited from mice that recognize unique linear and conformational epitopes of protein P7; (2) Three of the four Mabs derived from distinct B-cell clones utilize identical Vκ and VH genes of the same gene family demonstrating restricted gene utilization, while one, 6C3, which recognizes a conformational epitope, utilizes VH and Vκ genes frequently associated with anti-DNA antibodies; and (3) monoclonal antibodies can be utilized alone or in combination as structural probes for the disassembly or assembly of the ϕ6 nucleocapsid during transcription in the standard early-stage reaction conditions. During NC transcription P7 is in an exposed position or in proximity to the NC surface which is accessible to antibodies.
Conceived and designed the experiments: PG LS AK. Performed the experiments: AA HW. Analyzed the data: AA HW AK LS PG. Contributed reagents/materials/analysis tools: AA HW LS PG. Wrote the paper: AK LS PG.
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