Ligand Binding to the FA3-FA4 Cleft Inhibits the Esterase-Like Activity of Human Serum Albumin

Paolo Ascenzi; Loris Leboffe; Alessandra di Masi; Viviana Trezza; Gabriella Fanali; Magda Gioia; Massimo Coletta; Mauro Fasano

doi:10.1371/journal.pone.0120603

Abstract

The hydrolysis of 4-nitrophenyl esters of hexanoate (NphOHe) and decanoate (NphODe) by human serum albumin (HSA) at Tyr411, located at the FA3-FA4 site, has been investigated between pH 5.8 and 9.5, at 22.0°C. Values of K_s, k₊₂, and k₊₂/K_s obtained at [HSA] ≥ 5×[NphOXx] and [NphOXx] ≥ 5×[HSA] (Xx is NphOHe or NphODe) match very well each other; moreover, the deacylation step turns out to be the rate limiting step in catalysis (i.e., k₊₃ << k₊₂). The pH dependence of the kinetic parameters for the hydrolysis of NphOHe and NphODe can be described by the acidic pK_a-shift of a single amino acid residue, which varies from 8.9 in the free HSA to 7.6 and 7.0 in the HSA:NphOHe and HSA:NphODe complex, respectively; the pK>_a-shift appears to be correlated to the length of the fatty acid tail of the substrate. The inhibition of the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and 4-nitrophenyl myristate (NphOMy) by five inhibitors (i.e., diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol) has been investigated at pH 7.5 and 22.0°C, resulting competitive. The affinity of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol for HSA reflects the selectivity of the FA3-FA4 cleft. Under conditions where Tyr411 is not acylated, the molar fraction of diazepam, diflunisal, ibuprofen, and 3-indoxyl-sulfate bound to HSA is higher than 0.9 whereas the molar fraction of propofol bound to HSA is ca. 0.5.

Citation: Ascenzi P, Leboffe L, di Masi A, Trezza V, Fanali G, Gioia M, et al. (2015) Ligand Binding to the FA3-FA4 Cleft Inhibits the Esterase-Like Activity of Human Serum Albumin. PLoS ONE 10(3): e0120603. https://doi.org/10.1371/journal.pone.0120603

Academic Editor: Eugene A. Permyakov, Russian Academy of Sciences, Institute for Biological Instrumentation, RUSSIAN FEDERATION

Received: November 6, 2014; Accepted: January 24, 2015; Published: March 19, 2015

Copyright: © 2015 Ascenzi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: This work was supported by Ministero dell’Istruzione, dell’Università e della Ricerca of Italy (Università Roma Tre, Roma, Italy; CLAR 2014 to PA) and Consorzio Interuniversitario Italiano per l’Argentina (CUIA 2014 to PA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

The (pseudo-)enzymatic activity of human serum albumin (HSA) was first reported in 1951 and investigated extensively for decades. Among others, HSA displays esterase, RNA-hydrolyzing, enolase, glucuronidase, lipid peroxidase, aldolase glutathione-linked thiol peroxidase, and anti-oxidant activities. Moreover, heme binding confers to HSA globin-like (pseudo-)enzymatic properties, including detoxification of reactive nitrogen and oxygen species as well as catalase and peroxidase activities. Remarkably, HSA(-heme) (pseudo-)enzymatic properties are modulated allosterically and inhibited competitively [1,2].

Although the physiological importance of the esterase activity of HSA is obscure and the natural substrates are still unknown, HSA displays esterase activity towards several substrates including 4-nitrophenyl acetate (NphOAc), α-naphthyl acetate, phenyl acetate, 1-naphthyl N-methylcarbamate, β-naphthyl acetate, aspirin, ketoprofen glucuronide, carprofen acylglucuronide, cyclophosphamide, nicotinate esters, long and short-chain FA esters (e.g., 4-nitrophenyl myristate; NphOMy), octanoyl ghrelin, organophosphorus pesticides, carbaryl, 2-nitrotrifluoroacetanilide, 2-nitroacetanilide, and nerve agents [1–3].

The Lys199 and Tyr411 residues, placed in the fatty acid (FA) 7 (FA7) and 3–4 (FA3-FA4) site, respectively, are pivotal for the esterase activity of HSA; however, mechanisms for the Lys199- and Tyr411-assisted catalysis are substantially different [1–8].

At Lys199, the substrate (e.g., acetylsalicylic acid, trinitrobenzeno-sulfonates, and penicillin) is cleaved in two products; while one product is released, the other one binds covalently to the Lys199 residue [1,4,7]. Although the molecular mechanism underlying the Lys199 acetylation is unknown, it seems that its ability to attack the substrate is due to the proximity of the Lys195 residue, these two residues playing a combined and comparable chemical role. In fact, the basic form of Lys199 is likely connected to the acid form of Lys195 through a network of H-bonding water molecules with a donor-acceptor character. The presence of these water bridges is relevant for stabilizing the configuration of the FA7 site and/or promoting a potential Lys195-Lys199 proton-transfer process [6]. Since Lys199 is placed at the entrance of the FA7 site (i.e., Sudlow’s site I), ligand binding inhibits the Lys199-dependent esterase activity [3,8].

The catalytic mechanism involving the Tyr411 residue appears to be substrate-dependent. Of note, the hydrolysis of the most suitable substrate 4-nitrophenyl propionate leads to the release of both 4-nitrophenol and propionate [9]. This mechanism also applies to the hydrolysis of N-trans-cinnamoylimidazoles [10] and 4-nitrophenil esters of amino acids [11]. However, the Tyr411-assisted hydrolysis of NphOAc and NphOMy leads to the release of 4-nitrophenol and to Tyr411-acetylation and-myristoylation, respectively [12,13]. The strong nucleophilic nature of the phenolic oxygen of the Tyr411 residue is due to the close proximity of the Arg410 guanidine moiety that electrostatically stabilizes the reactive anionic form of the Tyr411 residue [5,14]. Since both the Arg410 and Tyr411 residues are placed in the FA3-FA4 site (i.e., Sudlow’s site II), ligand binding inhibits the HSA esterase activity [3,9,12,13]. Remarkably, the esterase activity of HSA could play a role in the inactivation of several toxins including organophosphorus compounds [3].

Present study largely extends previous investigations concerning the hydrolysis of 4-nitrophenyl esters by HSA [9,12–14]. In particular, kinetics of the HSA pseudo-enzymatic hydrolysis of 4-nitrophenyl hexanoate (NphOHe) and 4-nitrophenyl decanoate (NphODe) have been investigated between pH 5.8 and 9.5, under conditions where [HSA] ≥ 5×[NphOXx] and [NphOXx] ≥ 5×[HSA] (Xx indicates He or De). The rationale behind this selection is to investigate how the FA tail length affects the pK_a values of the ionizing group that modulates the catalysis. Furthermore, diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol have been reported to inhibit competitively the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and 4-nitrophenyl myristate (NphOMy) (see [12] and present study). Remarkably, the molar fraction of diazepam, diflunisal, ibuprofen, and 3-indoxyl-sulfate bound to not acylated HSA is higher than 0.9 whereas the molar fraction of propofol bound to HSA is ca. 0.5.

Materials and Methods

NphOHe, NphODe, NphOMy, 4-nitrophenol (NphOH), diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, propofol, and 1,3-bis(tris(hydroxymethyl)methylamino)propane (Bis-tris propane) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical or reagent grade and were used without further purification.

HSA (from Sigma-Aldrich, St Louis, MO, USA) was essentially FA free and was used without further purification. The HSA stock solution ([HSA] = 1.2×10^-2 M) was prepared by dissolving HSA in 2.0×10^-2 M Bis-tris propane buffer solution (pH 7.5). The HSA concentration was determined spectrophotometrically at 279 nm (ε = 3.6×10⁴ M^–1 cm^–1) [1]. Then, the HSA stock solution was diluted in the Bis-tris-propane buffer (0.1 M), at the desired pH; the final pH ranged between 5.8 and 9.5. The final HSA concentration ranged between 2.0×10^-6 M and 10×10^-4 M.

The NphOHe, NphODe, and NphOMy solutions were prepared by dissolving the substrates in a 2.0×10^-2 M Bis-tris propane buffer solution (pH 7.5) in the presence of 10% acetonitrile. The NphOHe, NphODe, and NphOMy concentration was determined spectrophotometrically at 400 nm (ε = 1.8×10⁴ M^–1 cm^–l; pH > 8.5 and 22.0°C), allowing to calculate the amount of 4-nitrophenol released from the substrate [15]. The final NphOHe, NphODe, and NphOMy concentration ranged between 2.0×10^-6 M and 1.0×10^-4 M. The final acetonitrile concentration was 0.5% (v/v) [9,12,13].

Kinetics and thermodynamics of the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy were followed spectrophotometrically at 405 nm by rapid mixing the HSA solution with the NphOHe, NphODe, and NphOMy solutions [9,12,13,15].

Kinetics and thermodynamics of the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy, obtained under conditions where [NphOXx] ≥ 5×[HSA] and [HSA] ≥ 5×[NphOXx], were analyzed in the framework of the minimum three step-mechanism reported in Fig. 1 [9,12–16].

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Fig 1. The minimum three step-mechanism for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy.

HSA is the substrate-free protein, NphOXx is the substrate, HSA:NphOXx is the reversible protein-substrate complex, HSA-OXx is considered to be an ester formed between the acyl moiety of the substrate and the O atom of the Tyr411 phenoxyl group [14], XxOH is hexanoate or decanoate or myristate, K_s is the pre-equilibrium constant for the formation of the HSA:NphOXx complex, k₊₂ is the first-order acylation rate constant, and k₊₃ is the first-order deacylation rate constant. Xx indicates Ac or He or De or My.

https://doi.org/10.1371/journal.pone.0120603.g001

Kinetics and thermodynamics of the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy were also determined in the presence of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate and propofol; the ligand concentration ranged between 4.0×10^-6 M and 8.0×10^-3 M.

Kinetic and thermodynamic parameters were obtained between pH 5.8 and 9.5, at 22.0°C.

Experiments were carried out with the SFM-200 rapid-mixing stopped-flow apparatus (BioLogic Science Instruments, Claix, France) and the Cary 50 Bio spectrophotometer (Varian Inc., Palo Alto, CA, USA).

Kinetics and thermodynamics of the HSA pseudo-esterase activity were analyzed using the GraphPad Prism program (GraphPad Software, Inc., La Jolla, CA, USA). The results are given as mean values of at least four experiments plus or minus the corresponding standard deviation.

Results

Pseudo-esterase activity of HSA-Tyr411

As previously reported for the HSA-Tyr411-catalyzed hydrolysis of NphOAc and NphOMy [12,13], the determination of kinetic parameters of Fig. 1 is simplified by the fact that the formation of the HSA:NphOHe and HSA:NphODe complexes can be treated as a rapid equilibrium process. Indeed, no lag phase occurs in the release of NphOH from NphOHe and NphODe in the presence of HSA (Fig. 2), indicating that the equilibration of HSA:NphOHe and HSA:NphODe with HSA and NphOHe and NphODe, respectively, is complete within 1.4 ms (i.e., the “dead-time” of the rapid-mixing stopped-flow apparatus). Moreover, the rate of NphOH release from NphOHe and NphODe catalyzed by HSA-Tyr411 is unaffected by the addition of NphOH (up to 1.0×10^-4 M) in the reaction mixtures (data not shown), indicating that the acylation step is essentially irreversible. If NphOH had affected the HSA-Tyr411 catalyzed hydrolysis of NphOHe and NphODe, the classical product (i.e., NphOH) inhibition behavior would have been observed.

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Fig 2. HSA-mediated hydrolysis of NphOHe and NphODe.

Time course of the reaction of 2.0×10^-6 M NphOHe (panel A) and NphODe (panel B) with 5.0×10^-5 M HSA, i.e. [HSA] ≥ 5×[NphOHe] and [HSA] ≥ 5×[NphODe], respectively. The continuous lines were calculated according equation (1) with k_app = (1.8±0.2)×10^-3 s^–1 (panel A) and (5.8±0.6)×10^-4 s^–1 (panel B). Dependence of k_app on the HSA concentration at [HSA] ≥ 5×[NphOHe] (panel C) and [HSA] ≥ 5×[NphODe] (panel D), respectively. The continuous lines were obtained according to equation (2) with the following parameters k₊₂ = (2.8±0.3)×10^-3 s^–1 and K_s = (2.9±0.3)×10^-5 M (panel C), and k₊₂ = (8.1±0.8)×10^-4 s^–1 and K_s = (2.3±0.3)×10^-5 M (panel D). [NphOHe] and [NphODe] was 2.010^-6 M and [HSA] ranged from 1.0×10^-5 M to 1.0×10^-4 M. Time course of the reaction of 2.0×10^-6 M HSA with 5.0×10^-4 M NphOHe (panel E) and NphODe (panel F)., i.e. [NphOHe] ≥ 5×[HSA] and [NphOHe] ≥ 5×[HSA], respectively. The continuous lines were calculated according to equation (6) with k_obs = (1.9±0.2)×10^-3 s^–1 (panel E) and (4.8±0.5)×10^-4 s^–1 (panel F). Dependence of k_obs on the NphOHe and NphODe concentration at [NphOHe] ≥ 5×[HSA] (panel G) and [NphODe] ≥ 5×[HSA] (panel H). The continuous lines were obtained according to equation (6) with the following parameters k₊₂ = (2.9±0.3)×10^-3 s^–1 and K_s = (2.8±0.3)×10^-5 M (panel G), and k₊₂ = (7.1±0.8)×10^-4 s^–1 and K_s = (2.2±0.2)×10^-5 M (panel H). The value of k₊₃ approximates to 0 s^–1. [HSA] was 2.0×10^-6 M, and [NphOHe] and [NphODe] ranged from 1.0×10^-5 M to 1.0×10^-4 M. Where not shown, the standard deviation is smaller than the symbol. For details, see text.

https://doi.org/10.1371/journal.pone.0120603.g002

When [HSA] ≥ 5×[NphOHe] and [HSA] ≥ 5×[NphODe], the reaction of HSA with NphOHe and NphODe displays a mono-exponential time-course (Fig. 2, panels A and B). Values of the pseudo-first order rate constant for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe (i.e., of NphOH release; k_app) were obtained according to equation (1) [9,12,13]: (1) where Xx is De or He. Values of k_app were independent of the NphOHe and NphODe concentration under conditions where [HSA] ≥ 5×[NphOHe] and [HSA] ≥ 5×[NphODe].

Values of K_s and k₊₂ for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe (see Table 1) were obtained from the hyperbolic plots of k_app as a function of the HSA concentration (Fig. 2, panels C and D) according to equation (2) [9,12,13]:

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Table 1. Values of catalytic parameters for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy, at pH 7.5 and 22.0°C.

https://doi.org/10.1371/journal.pone.0120603.t001

(2)

When [NphOHe] ≥ 5×[HSA] and [NphODe] ≥ 5×[HSA], the reaction of NphOHe and NphODe with HSA displays a mono-exponential time course (Fig. 2, panels E and F). Values of the pseudo-first-order rate constant for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe (i.e., of NphOH release; k_obs) were obtained according to equation (3) [9,12,13]:

(3)

Values of k_obs are independent of the HSA concentration when [NphOHe] ≥ 5×[HSA] and [NphODe] ≥ 5×[HSA].

When k₊₂ ≥ 5×k₊₃, the differential equations arising from Fig. 1 may be solved [12,13,16,17] to describe the time course of NphOH release at the early stages of the reaction. The resulting expression is given in eqs (4)–(6) [12,13,16,17]: (4) where (5) and (6)

As predicted from eqs (4)–(6), a “burst” phase of NphOH release of amplitude α with the first order rate constant k_obs occurs. Values of α, obtained at [NphOHe] ≥ 5×[HSA] and [NphODe] ≥ 5×[HSA], range between 0.99 and 1.03 (S1 Table), indicating that the HSA:NphOXx:NphOH stoichiometry is 1:1:1. Moreover, the time course of the “burst” phase of NphOH release is a first-order process for more than 95% of its course (Fig. 2, panels E and F) as estimated from residual analysis. Values of k_obs are independent of the HSA concentration when [NphOHe] ≥ 5×[HSA] and [NphODe] ≥ 5×[HSA]. Values of K_s and k₊₂ (see Table 1) were determined from hyperbolic plots of k_obs versus [NphOXx] (Fig. 2, panels G and H) according to equation (6) [16,17]. Under all the experimental conditions, the y-intercept of the hyperbola described by equation (6) was < 2×10^-6 s^–1, thus indicating that the value of k₊₃ is at least 100-fold smaller than that of k_obs obtained at the lowest NphOHe and NphODe concentration (i.e., k₊₃ < 2×10^-6 s^–1).

As predicted from Fig. 1, values of K_s and k₊₂ obtained under conditions where [HSA] ≥ 5×[NphOHe] and [HSA] ≥ 5×[NphODe] from equation (2) are in excellent agreement with those obtained under conditions where [NphOHe] ≥ 5×[HSA] and [NphODe] ≥ 5×[HSA] from equation (6) (Table 1). Moreover, data here reported indicate that the deacylation process is rate limiting in the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe, as previously reported for NphOAc and NphOMy [12,13] (i.e., k₊₃ << k₊₂). Furthermore, values of K_s and k₊₂ here obtained for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe agree with those previously reported [9].

pH effects on the pseudo-esterase activity of HSA-Tyr411

Values of catalytic parameters for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe obtained between pH 5.8 and 9.5 (at 22.0°C) are summarized in S2 and S3 Tables. Fig. 3 shows the pH dependence of K_s, k₊₂, and k₊₂/K_s values for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODe. Values of pK_a modulating the pH dependence of k₊₂/K_s, k₊₂, and K_s were determined by data analysis according to eqs (7)–(9) [9,12,13,15–17]: (7) (8) (9) where K_s^lim, k₊₂^lim, and (k₊₂/K_s)^lim are the values corresponding to the alkaline asymptotes of K_s, k₊₂, and k₊₂/K_s.

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Fig 3. pH dependence of Log K_s (panels A and B), k₊₂ (panels C and D), and k₊₂/K_s (panels E and F) for HSA-mediated hydrolysis of NphOHe (panels A, C, and E) and NphODe (panels B, D, and F), at 22.0°C.

Circles indicate data obtained under conditions where [HSA]≥5×[NphOHe] and [HSA]≥5×[NphODe]. Squares indicate data obtained under conditions where [NphOHe]≥5×[HSA] and [NphOHe]≥5×[HSA]. The continuous lines in panels A and B, C and D, and E and F were obtained from data analysis according to eqs (7)–(9), respectively, with values of parameters given in Table 2. Where not shown, the standard deviation is smaller than the symbol. For details, see text.

https://doi.org/10.1371/journal.pone.0120603.g003

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Table 2. pK_unl and pK_lig values as well as of alkaline limiting values of k₊₂, K_s, and k₊₂/K_s for the HSA-Tyr411-catalyzed hydrolysis of NphOAc; NphOHe, NphODe, and NphOMy, at 22.0°C.

https://doi.org/10.1371/journal.pone.0120603.t002

According to linked functions [12,13,16–18], the pH dependence of K_s reflects the acidic pK_a-shift of a single amino acid residue from free HSA (i.e., pK_unl) to the HSA:NphOHe and HSA:NphODe complexes (i.e., pK_lig). Moreover, the pH dependence of k₊₂ and k₊₂/K_s reflects the acid-base equilibrium of one apparent ionizing group in the HSA:NphOHe and HSA:NphODe complexes (i.e., pK_lig) and in free HSA (i.e., pK_unl), respectively. As expected [12,13,16–18], the pK_a value of free HSA (i.e., pK_unl) is independent of the substrate whereas the pK_a values of the HSA:NphOHe and HSA:NphODe complexes (i.e., pK_lig) depend on the substrate (Table 2).

Competitive inhibition of the pseudo-esterase activity of HSA-Tyr411

As shown in Fig. 4, diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol inhibit the Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy. As expected for the pure competitive inhibition mechanism [19], values of K_s for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, NphODe, and NphOMy (Fig. 4) increase with the diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol concentration whereas values of k₊₂ are unaffected by the ligand concentration (S1, S2, and S3 Figs.). The analysis of the linear dependence of the K_s^app/K_s ratio on the ligand concentration (i.e., [ligand]) according to equation (10) [19]: (10) allowed to determine the values of the equilibrium dissociation constant for diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol binding to the FA3-FA4 cleft of HSA (i.e., K_I, corresponding to the absolute value of the x intercept of the linear plot). As expected for the pure competitive inhibition mechanism [19], values of K_I for diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and/or propofol binding to the FA3-FA4 cleft of HSA are independent of NphOAc [13], NphOHe (present study), NphODe (present study), and NphOMy (see [12] and present study) (Fig. 5). Values of K_I for diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol binding to HSA here obtained (Fig. 5) agree with those reported previously [2,12,13,20–26].

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Fig 4. Competitive inhibitory effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol on the HSA-Tyr411-catalyzed hydrolysis of NphOHe (squares), NphODe (circles), and NphOMy (triangles).

Data were obtained under conditions where [NphOHe] > 5×[HSA]. The analysis of data according to equation (10) allowed to determine values of K_I (indicated by arrows) reported in Fig. 5. Where not shown, the standard deviation is smaller than the symbol. For details, see text.

https://doi.org/10.1371/journal.pone.0120603.g004

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Fig 5. Values of K_I for the competitive inhibition of the HSA-Tyr411-catalyzed hydrolysis of NphOAc, NphOHe, NphODe, and NphOMy by diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and/or propofol, at pH 7.5 and 22.0°C.

^a From [13]. ^b Present study. ^c From [12].

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Discussion

The hydrolysis of NphOAc, NphOHe, NphODe, and NphOMy by HSA-Tyr411 (see [12–15] and present study) is reminiscent of that observed for acylating agents with proteases [27]. In fact, NphOAc [13], NphOHe (present study), NphODe (present study), and NphOMy [12] act as suicide substrates of HSA-Tyr411, values of the deacylation rate constant for all four substrates (i.e., k₊₃) being lower by several orders of magnitude than those of the acylation rate constant (i.e., k₊₂). Remarkably, HSA acylation appears to modulate ligand binding. In fact, HSA acylation by aspirin [28] increases the affinity of phenylbutazone and inhibits bilirubin and prostaglandin binding, thus accelerating the clearance of prostaglandins, which represents an additional mechanism of the aspirin anti-inflammatory action [29].

Kinetics for the hydrolysis of NphOHe and NphODe by HSA are pH dependent, reflecting the acidic pK_a shift of an apparently single ionizing group of HSA upon substrate binding. This could reflect the reduced solvent accessibility of the Tyr411 residue, representing the primary esterase site of HSA (see [9,12–14]), although long range effects could not be ruled out. The Tyr411 catalytic residue is located in the FA3-FA4 cleft, which is made by an apolar region forming the FA3 site and a polar patch contributing the FA4 site. The polar patch is centered on the Tyr411 side chain and includes Arg410, Lys414, and Ser489 residues [8,30,31]. The inspection of the three-dimensional structure of the ligand-free HSA [32] and of the molecular model of the HSA:4-nitrophenyl propionate complex [9] suggests that the observed pH effects (Fig. 3) could reflect the acidic pK_a shift of the Tyr411 residue upon substrate binding. This would render more stable the negative charge on the phenoxyl O atom of Tyr411, which appears to be hydrogen bonded to the carbonyl O atom of 4-nitrophenyl propionate [9], potentiating its nucleophilic role as an electron donor in the pseudo-esterase activity of HSA. Of note, the acidic shift of the pK_a value of the ionizing group affecting catalysis from 8.9±0.1 in ligand free-HSA to 8.1±0.2, 7.6±0.2, 7.0±0.2, and 6.8±0.2 in the HSA:NphOAc, HSA:NphOHe HSA:NphODe, and HSA:NphOMy complexes (see Table 2), respectively, depends on the length of the fatty acid tail. Therefore, it appears as the increase of the FA tail length brings about the progressive reduction of the water solvent accessibility, thus enhancing the hydrophobicity of the catalytic site and leading to a decreased pK_a of the ionizing group modulating the catalysis. Of note, the pH dependence of the Tyr411-associated esterase activity parallels the pH-dependent neutral-to-basic allosteric transition of HSA [3].

Diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and/or propofol inhibit competitively the hydrolysis of NphOAc [13], NphOHe (present study), NphODe (present study), and NphOMy (see [12] and present study) (Fig. 5) by impairing the accessibility of 4-nitroplenyl esters to the Tyr411 catalytic center. In particular, diazepam, diflunisal, ibuprofen, and 3-indoxyl-sulfate bind to the center of the FA3-FA4 cleft, with one O atom being hydrogen bonded to the Tyr411 OH group (Fig. 6). On the other hand, propofol binds to the apolar region of the FA3-FA4 cleft with the phenolic OH group making a hydrogen bond with the carbonyl O atom of Leu430. Moreover, the aromatic ring of the propofol is sandwiched between the Asn391 and Leu453 side chains. Furthermore, one of the two isopropyl groups of propofol makes several apolar contacts at one end of the pocket, whereas the other is solvent exposed at the cleft entrance making close contacts with Asn391, Leu407, Arg410, and Tyr411 (Fig. 6) [8,30,31].

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Fig 6. Three-dimensional structures of the HSA:diazepam, :diflunisal, :ibuprofen, :3-indoxyl-sulfate, and :propofol complexes.

The ligands are shown in the ball-and-stick representation. The Tyr411 phenoxyl O atom of HSA is hydrogen bond to a O atom of diazepam, diflunisal, ibuprofen, and 3-indoxyl-sulfate. The Leu430 carbonyl O atom of HSA is hydrogen bond to the O atom of propofol. The hydrogen bonds are represented as red dashed lines. The PDB ID codes of HSA:diazepam, :diflunisal, :ibuprofen, :3-indoxyl-sulfate, and :propofol complexes are 2BXE, 2BXF, 2BXG, 2BXH, and 1E7A, respectively [8,30]. The pictures were drawn with the UCSF-Chimera package [46]. For details, see text.

https://doi.org/10.1371/journal.pone.0120603.g006

The different K_I values for diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol binding to HSA (Fig. 5) agree with the selectivity of the FA3-FA4 cleft of HSA, which can be ascribed to the presence of a basic polar patch located at one end of the apolar FA3-FA4 cleft. Remarkably, diazepam, diflunisal, ibuprofen, and 3-indoxyl-sulfate are oriented with at least one O atom in the vicinity of the polar patch. On the other hand, the single polar hydroxyl group in the center of propofol does not interact with the polar patch of the FA3-FA4 cleft. Moreover, the FA3-FA4 cleft appears to adopt different ligand-dependent shapes, thus paying different free energy contributions for structural rearrangements [8].

Conclusion

Due to the relevant physiological role of HSA in human plasma, in vivo implications can be argued from the present results. Accounting for the plasma levels of HSA (i.e., [HSA] = 7.5×10^-4 M) [2] as well as of diflunisal, ibuprofen, and 3-indoxyl-sulfate plasma levels (i.e., [ligand]; see below) and K_I values for ligand binding (Fig. 4 and Fig. 5), the molar fraction (i.e., Y) of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulfate, and propofol bound to plasmatic HSA was calculated according to equation (11) [33]:

(11)

The following therapeutic plasma levels of the drugs investigated are commonly reported at steady-state: (i) 0.3–0.4 μg/mL (i.e., 1.1×10^-6 M to 1.1×10^-6 M) of diazepam are recommended for anxiolytic effects, and ca. 0.6 μg/mL (i.e., 2.1×10^-6 M) for control of seizures; higher concentrations might suggest misuse or abuse [34–36]; (ii) 90–110 μg/mL (i.e., 3.6×10^-4 M to 5.3×10^-4 M) of diflunisal are usually required for anti-inflammatory effects [37,38]; (iii) 20–40 μg/mL (i.e., 9.7×10^-5 M to 1.9×10^-4 M) are observed after a single oral anti-inflammatory dose of ibuprofen [39,40]; and (iv) 1–2 μg/mL (i.e., 5.6×10^-6 M to 1.1×10^-5 M) of propofol are necessary to maintain sleep [41,42]. Conversely, 3-indoxyl-sulfate is an uremic toxin accumulated in the plasma of chronic kidney disease patients and induces an oxidative stress in a variety of cells such as renal tubular cells, glomerular mesangial cells, vascular smooth muscle cells, vascular endothelial cells, and osteoblasts. Under pathological conditions, the plasma concentration of 3-indoxyl-sulfate ranges between 4 and 60 μg/mL (i.e., 1.9×10^-5 M to 2.8×10^-4 M) [43,44].

Since the plasma levels of diflunisal, ibuprofen, and 3-indoxyl-sulfate (see above) are higher than values of K_I for ligand binding to HSA by about 100 folds (see Fig. 4 and Fig. 5), the molar fraction of diflunisal, ibuprofen, and 3-indoxyl-sulfate bound to HSA is higher than 0.9, according to equation, (11). Although the commonly reported diazepam and propofol plasma levels (see above) are lower than the corresponding values of K_I for drug binding to HSA (see Fig. 4 and Fig. 5) by about 5 and 100 folds, respectively, the plasma HSA concentration (see above) is higher than K_I for by about 70 and 2 folds, respectively. According to equation (11), the molar fraction of diazepam and propofol bound to HSA in plasma is higher than 0.9 and 0.5, respectively.

As a whole, data here reported highlight the role of drugs diazepam, diflunisal, ibuprofen, and propofol as well as of the uremic toxin 3-indoxyl-sulfate to inhibit competitively the pseudo-esterase activity of HSA, Tyr411 representing the nucleophile. This aspect is appropriate since HSA acylation appears to modulate ligand binding [28,29] and the detoxification of several compounds [2,3]. Last, HSA not only acts as a carrier and as a detoxifier but also displays transient drug- and toxin-based properties, representing a case for “chronosteric effects” [45]. This opens the scenario toward the possibility of a drug- and toxin-dependent multiplicity of roles for HSA.

Supporting Information

S1 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k₊₂ value for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, at pH 7.5 and 22.0°C.

The filled square on the ordinate indicates the k₊₂ value obtained in the absence of the ligands.

https://doi.org/10.1371/journal.pone.0120603.s001

(DOC)

S2 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k₊₂ value for the HSA-Tyr411-catalyzed hydrolysis of NphODe, at pH 7.5 and 22.0°C.

The filled square on the ordinate indicates the k₊₂ value obtained in the absence of the ligands.

https://doi.org/10.1371/journal.pone.0120603.s002

(DOCX)

S3 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k₊₂ value for the HSA-Tyr411-catalyzed hydrolysis of NphOMy, at pH 7.5 and 22.0°C.

The filled square on the ordinate indicates the k₊₂ value obtained in the absence of the ligands.

https://doi.org/10.1371/journal.pone.0120603.s003

(DOCX)

S1 Table. Values of α for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODE, at 22.0°C.

https://doi.org/10.1371/journal.pone.0120603.s004

(DOC)

S2 Table. Values of catalytic parameters for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, at 22.0°C.

https://doi.org/10.1371/journal.pone.0120603.s005

(DOCX)

S3 Table. Values of catalytic parameters for the HSA-Tyr411-catalyzed hydrolysis of NphODe, at 22.0°C.

https://doi.org/10.1371/journal.pone.0120603.s006

(DOC)

Author Contributions

Conceived and designed the experiments: PA VT MC MF. Performed the experiments: LL MG ADM. Analyzed the data: LL GF MG ADM. Contributed reagents/materials/analysis tools: PA. Wrote the paper: PA VT MF.

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Figures

Abstract

Introduction

Materials and Methods

Results

Pseudo-esterase activity of HSA-Tyr411

pH effects on the pseudo-esterase activity of HSA-Tyr411

Competitive inhibition of the pseudo-esterase activity of HSA-Tyr411

Discussion

Conclusion

Supporting Information

S1 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k+2 value for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, at pH 7.5 and 22.0°C.

S2 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k+2 value for the HSA-Tyr411-catalyzed hydrolysis of NphODe, at pH 7.5 and 22.0°C.

S3 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k+2 value for the HSA-Tyr411-catalyzed hydrolysis of NphOMy, at pH 7.5 and 22.0°C.

S1 Table. Values of α for the HSA-Tyr411-catalyzed hydrolysis of NphOHe and NphODE, at 22.0°C.

S2 Table. Values of catalytic parameters for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, at 22.0°C.

S3 Table. Values of catalytic parameters for the HSA-Tyr411-catalyzed hydrolysis of NphODe, at 22.0°C.

Author Contributions

References

S1 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k₊₂ value for the HSA-Tyr411-catalyzed hydrolysis of NphOHe, at pH 7.5 and 22.0°C.

S2 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k₊₂ value for the HSA-Tyr411-catalyzed hydrolysis of NphODe, at pH 7.5 and 22.0°C.

S3 Fig. Effect of diazepam, diflunisal, ibuprofen, 3-indoxyl-sulphate, and propofol on the k₊₂ value for the HSA-Tyr411-catalyzed hydrolysis of NphOMy, at pH 7.5 and 22.0°C.