A. Three cell states a,b,c with distinct gene expression (xa,ya),(xb,yb) and (xc,yc). B. The gene regulatory circuit of X and Y determines three cell states a,b,c. C. Each state is associated with a growth rate ga,gb,gc respectively. Three states transition to each other with the interconversion rates kab,kba,kac,kca,kbc,kcb.
There is an error in the first sentence of the seventh paragraph of subsection “Elementary model: two-phenotype cell population dynamics” of section “Cell Population Model for Transition and Growth Dynamics: Two-Phenotypes”. The correct sentence is: We can determine the population ratio r between the two subpopulations in this regime.
Leukemia cell line HL60 has two subpopulations, MDRHigh and MDRLow, based on their abilities to retain CalceinAM fluorescent dye (flow cytometry profiles), as measured by flow cytometry. The flow cytometry histograms correspond to a snapshot of the cell population at a given time point. In this particular case the parameter is the accumulation of a fluorescent dye, CalceinAM, which works as a surrogate for ABC transporters activity and multidrug resistance: if the cells retain the dye, ABC transporters are not active and the cell is sensitive to drug; if the cells do not accumulate the dye, ABC transporters are active and the cell is resistant to drug treatment. A. In the absence of drug the two subpopulations co-exist at a stable cell ratio, MDRHigh = 2% and MDRLow = 98%. B. When the cells are treated with 10 nM of vincristine for 72 h the proportions change to MDRHigh = 40% and MDRLow = 60%. For further details please refer to Pisco et al .
A. Illustration of cell growth and transition for breast cancer cell line with three distinct cell phenotypes: luminal cell, basal cell and mammary stem cell. B1-B6. After FACS sorting, each isolated subpopulation of cell line SUM159, stem-like, basal and luminal cells, re-equilibrate to the stable cell-state ratio r*. Upper panels are the dynamics for cell numbers of three subpopulations; Lower panels are the dynamics for the cell ratios of three subpopulations. C1-C6. After FACS sorting, each isolated subpopulation of cell line SUM149, stem-like, basal and luminal cells, re-equilibrate to the stable cell-state ratio r* Upper panels are the dynamics for cell numbers of three subpopulations; Lower panels are the dynamics for the cell ratios of three subpopulations.
There are errors in the Author Contributions. The correct contributions are: Conceived and designed the experiments: JZ, HQ and SH. Performed the experiments: JZ and AOP. Analyzed the data: JZ and AOP. Contributed reagents/materials/analysis tools: HQ and AOP. Wrote the paper: JZ and AOP. All authors participated in editing the manuscript.
Citation: The PLOS ONE Staff (2015) Correction: Nonequilibrium Population Dynamics of Phenotype Conversion of Cancer Cells. PLoS ONE 10(3): e0118133. https://doi.org/10.1371/journal.pone.0118133
Published: March 26, 2015
Copyright: © 2015 The PLOS ONE Staff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited