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Abstract
Neuroplasticity in the mesolimbic dopaminergic system is critical for behavioral adaptations associated with opioid reward and addiction. These processes may be influenced by cholinergic transmission arising from the laterodorsal tegmental nucleus (LDTg), a main source of acetylcholine to mesolimbic dopaminergic neurons. To examine this possibility we asked if chronic systemic morphine administration affects expression of genes in ventral and ventrolateral periaqueductal gray at the level of the LDTg using rtPCR. Specifically, we examined gene expression changes in the area of interest using Neurotransmitters and Receptors PCR array between chronic morphine and saline control groups. Analysis suggested that chronic morphine administration led to changes in expression of genes associated, in part, with cholinergic neurotransmission. Furthermore, using a quantitative immunofluorescent technique, we found that chronic morphine treatment produced a significant increase in immunolabeling of the cholinergic marker (vesicular acetylcholine transporter) in neurons of the LDTg. Finally, systemic administration of the nonselective and noncompetitive neuronal nicotinic antagonist mecamylamine (0.5 or 2 mg/kg) dose-dependently blocked the expression, and to a lesser extent the development, of locomotor sensitization. The same treatment had no effect on acute morphine antinociception, antinociceptive tolerance or dependence to chronic morphine. Taken together, the results suggest that endogenous nicotinic cholinergic neurotransmission selectively contributes to behavioral sensitization to morphine and this process may, in part, involve cholinergic neurons within the LDTg.
Citation: Bajic D, Soiza-Reilly M, Spalding AL, Berde CB, Commons KG (2015) Endogenous Cholinergic Neurotransmission Contributes to Behavioral Sensitization to Morphine. PLoS ONE 10(2): e0117601. https://doi.org/10.1371/journal.pone.0117601
Academic Editor: Yong-hui Dang, Xi’an Jiaotong University School of Medicine, CHINA
Received: April 3, 2014; Accepted: December 29, 2014; Published: February 3, 2015
Copyright: © 2015 Bajic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are within the paper.
Funding: Presented work was supported by Foundation for Anesthesia Education and Research, Endo Pharmaceuticals, and NIH R03 DA030874 (DB), Mayo Family Research Endowment Fund (CBB), and NIH R01 DA021801 (KGC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: Funding from Endo Pharmaceuticals does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials.
Introduction
Opioid analgesic abuse has evolved into a national epidemic as a significant clinical problem with devastating consequences [1]. The mesolimbic dopaminergic system originating from the ventral tegmental area (VTA), and its major projection to the anterior part of the basal forebrain, the nucleus accumbens, plays a central role in motivation, reward, and addiction of drugs of abuse, including morphine [2–6]. Specifically, high-frequency bursts of action potentials in VTA neurons are critical for reward-dependent learning [7]. Similarly, changes in the properties of excitatory synapses on VTA dopaminergic neurons (A10) [8] are considered by some the most important neural circuit changes that lead to the development of addiction [9].
However, neuroplasticity in mesolimbic circuitry following chronic administration of morphine remains under investigation. Previous work demonstrated that electrical stimulation of the cholinergic nucleus, laterodorsal tegmental nucleus (LDTg) located in the ventrolateral periaqueductal gray (PAG), promotes burst firing of VTA dopaminergic neurons and increases dopamine release in the nucleus accumbens [10,11]. Similarly, LDTg lesions attenuate dopamine efflux in nucleus accumbens [12] and striatum [13] in response to morphine. This is consistent with anatomical tracing studies, which revealed that the LDTg projects mainly to the VTA [14] and makes excitatory synapses on dopaminergic neurons projecting to the nucleus accumbens [15]. Interestingly, cholinergic neurons of LDTg/pedunculopontine tegmentum provide the only known cholinergic input to the VTA [14,16,17]. Furthermore, LDTg is located in the midbrain ventrolateral PAG, a major brainstem site of analgesic actions of systemic morphine [18,19]. Plasticity in the ventrolateral PAG appears critical not only for the development of antinociceptive tolerance [20–22] and physical dependence [23], but also for sensitization to morphine [24,25]. The latter corresponds to certain aspects of drug addiction in animal models. In our recent study [26], by using Fos immunohistochemistry, we showed that chronic systemic morphine exposure is associated with a region-specific neuroplasticity in the rat ventrolateral PAG. This effect was specific to a selective region of the ventrolateral PAG at the level of the inferior colliculus where LDTg nucleus is located. Since cholinergic neurons of the LDTg are in a position to critically influence the expression of reward and addictive behavior following chronic opioid exposure [27], we hypothesized that cholinergic neuroplasticity at the level of the LDTg can underlie, at least in part, the sensitization effects of morphine.
High-throughput studies that looked into potential gene networks associated with chronic morphine administration are limited [28]. Thus, our first goal was to analyze expression of a select panel of neurotransmitter-related genes in the ventrolateral and ventral PAG following chronic morphine treatment. We hypothesized that chronic morphine administration would be associated with gene expression changes involving, in part, cholinergic system. Furthermore, we used quantitative fluorescent immunohistochemistry to test the hypothesis that chronic morphine administration is associated with an increase of cholinergic marker, vesicular acetylcholine transporter (vAChT) at the level of the LDTg. Finally, our third goal was to examine a possible cholinergic nicotinic role on development of (1) analgesic tolerance, (2) locomotor sensitization, and (3) dependence using the nonspecific and noncompetitive neuronal nicotinic antagonist, mecamylamine. All together, presented results support the role of LDTg in enhancing reward and motor-activity function of midbrain dopamine neurons following chronic morphine administration.
Materials and Methods
2.1. Animal Care and Use
Adult male Sprague-Dawley rats (250–300 g) derived from Sasco (Charles River Laboratories International, Inc., Wilmington, MA) were maintained on a 12 hours light/dark cycle. Food and water were given ad libitum. The Institutional Animal Care and Use Committee at Boston Children’s Hospital approved the experimental protocols for the use of vertebrate animals in this study (IACUC#12–03–2172R). Experiments were conducted according to the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals and the Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80–23, revised 1996). All efforts were made to minimize the number of animals used and their discomfort.
2.2. Quantitative Gene Expression Analysis using Polymerase Chain Reaction (PCR) Array
Molecular analysis included two groups that received twice-daily (9 AM and 5 PM) subcutaneous (sc) injections for 6.5 days: (1) saline control group that received only normal saline, and (2) chronic morphine group that received only morphine (10 mg/kg; Baxter Healthcare Corp., Deerfield, IL). This injection protocol was previously described [26,29]. We used a total of 40 adult rats (5 animals/group with 3–4 replicates per group). One hour following the last injection in the morning of day 7, animals were deeply anesthetized with sodium pentobarbital 100 mg/kg, intraperitoneally (ip) and decapitated. This time point was selected because antinociceptive effects following systemic administration of morphine in rats peak at around 60 min [30]. Brains were removed, and coronal tissue blocks were dissected on ice. Dissected blocks included ventrolateral and ventral PAG at the level of the inferior colliculus, where LDTg and dorsal raphe nucleus are located, respectively (Fig. 1). Tissue blocks corresponded to distances from Bregma of-7.64 to-9.16 according to the adult rat brain atlas [31]. Average weight (mg ± SD) of dissected tissue block was 26 ± 18 mg. Tissue blocks from animals of the same group (n = 5 animals/replicate/group) were collected and homogenized in 1 ml of Trizol Reagent (Life Technologies Corp., NY) for total RNA isolation using the phenol-chloroform method [32], followed by addition of 0.2 ml chloroform per 1 ml Trizol used. After incubation for 3 minutes at room temperature, samples were centrifuged at 11.000 g for 15 minutes at 4°C. The upper aqueous phase was transferred to another vial, and RNA was precipitated by adding 1 mcL glycogen (Invitrogen, CA) and 0.5 ml isopropyl alcohol (Sigma, St. Louis, MO) per 1 ml Trizol used. After incubation of samples for 10 minutes at room temperature, separation of RNA was accomplished by centrifugation at 11.000 g for 15 minutes at 4°C. Pellets containing total RNA were washed once with 1 ml 75% ethanol centrifuged at 7.500 g for 5 minutes at 4°C. After that, pellets containing washed RNA were dried for 3 minutes at room temperature, and then dissolved in RNAse-free water (50 μl water per 100 mg brain tissue homogenized). Samples were aliquoted and stored at-80°C. The RNA yield was evaluated in all the samples by analyzing the spectrophotometric ratios 260/280 using a Nanodrop 2000c Spectrophotometer (ThermoScientific, West Palm Beach, FL). RNA integrity was corroborated using 2% agarose gels with ethidium bromide (3 μl/50 ml TRIS) and visualized in a UV transilluminator, showing two main bands corresponding to 18S and 28S rRNAs (data not shown). Samples containing a minimum of 5 mcg of RNA (at a concentration greater than 0.5 mg/ml) were sent on dry ice to SABiosciences (Frederick, MD) for quantitative PCR analysis using The Rat Neurotransmitter Receptors and Regulators RT² Profiler PCR Array (PARN-060; SABiosciences, a QIAGEN company). The Rat Neurotransmitter Receptors and Regulators PCR Array profiles the expression of 84 genes involved in modulating the biological processes of neurotransmitter biosynthesis, uptake, transport, and signaling through neurotransmitter receptors. This array contains receptors for specific neurotransmitters, such as acetylcholine, benzodiazepine, dopamine, gamma-aminobutyric acid (GABA), glutamate, serotonin, somatostatin and several neuropeptides. Genes involved in the regulation of neurotransmitter levels are included as well. Refer to Table 1 for the list of all 84 genes included in the Array and analyzed in this study. Reported genes included genes exhibiting any expression change that was significant (p<0.05) or had more than 2-fold change (100%) but was statistically marginally non-significant (p = 0.05–0.2).
Schematic drawing illustrates rat transverse midbrain section at the level of the inferior colliculus (IC). It corresponds to Plates 52–56 of the adult rat brain atlas [31]. Rectangle encloses area dissected for the isolation of the tissue used in molecular experiments. It includes laterodorsal tegmental nucleus (LDTg) of the ventrolateral periaqueductal gray (vlPAG) and dorsal raphe (DR). Numbers in the upper right corner illustrate distance from Bregma. Abbreviations: Aq, aqueduct (Sylvius); CnF, cuneiform nucleus; LDTgV, laterodorsal tegmental nucleus, ventral part; PnO, pontine reticular nucleus, oral part.
Gene Expression Changes in Ventral and Ventrolateral Periaqueductal Gray with Chronic Morphine Treatment at the level of Inferior Colliculus (for anatomical location of region of analysis, see Fig. 1). Out of 84 total genes in the Neurotransmitter Receptors and Regulators PCR Array (SABiosciences, MD), 4 genes showed statistically significant change (p<0.05), while additional 4 genes more than two-fold (100%) change that was statistically marginally non-significant (p = 0.05–0.2; two-tailed t-test). Total of 5 housekeeping genes were used as a reference and showed no changes between treatments. These included: Actb, Hprt1, Ldha, Rpl13a, Rplp1. Gene nomenclature was adopted from SABiosciences and corresponds to PubMed. Abbreviations: Abat, 4-aminobutyrate aminotransferase; AchE, acetylcholinesterase; Anxa9, annexin A9;Brs3, Bombesin-like receptor 3; Cckar, cholecystokinin A receptor; Cckbr, cholecystokinin B receptor; Chat, choline acetyltransferase; Chrm1–5, cholinergic receptor, muscarinic 1–5 (muscle); Chrna1–6, cholinergic receptor, nicotinic, alpha 1–6 (neuronal); Chrnb1–4, cholinergic receptor, nicotinic, beta 1; Chrnd, cholinergic receptor, nicotinic, delta; Chrne, cholinergic receptor, nicotinic, epsilon; Chrng, cholinergic receptor, nicotinic, gamma; Comt, catechol-O-methyltransferase; Drd1a, dopamine receptor D1A; Drd2–5, dopamine receptor D2–5; Gabra1–6, gamma-aminobutyric acid (GABA) A receptor, alpha 1–6; Gabrb2–3, gamma-aminobutyric acid (GABA) A receptor, beta 2–3; Gabrd, gamma-aminobutyric acid (GABA) A receptor, delta; Gabre, gamma-aminobutyric acid (GABA) A receptor, epsilon; Gabrg1–2, gamma-aminobutyric acid (GABA) A receptor, gamma 1–1; Gabrp, gamma-aminobutyric acid (GABA) A receptor, pi; Gabrq, gamma-aminobutyric acid (GABA) A receptor, theta; Gabrr1–2, gamma-aminobutyric acid (GABA) A receptor, rho 1–2; Gad1–2, glutamate decarboxylase 1–2; Galr1–3, galanin receptor 1–3; Glra1–3, glycine receptor, alpha 1–3; Glrb, glycine receptor, beta; Grpr, gastrin releasing peptide receptor; Htr3a, 5-hydroxytryptamine (serotonin) receptor 3a; Maoa, monoamine oxidase A; Mc2r, melanocortin 2 receptor; Nmur1–2, neuromedin U receptor 1–2; Npffr1–2, neuropeptides FF receptor1–2; Npy1r-2r, 5r, neuropeptide Y receptor 1–2,5; Ppyr1, pancreatic polypeptide receptor 1; Prima1, proline rich membrane anchor 1; Prlhr, prolactin releasing hormone receptor; Prokr1–2, prokineticin receptor 1–2; Qrfpr, proglutamylated RFamide peptide receptor; Slc5a7, solute carrier family 5 (choline transporter) member 7; Sstr1–5, somatostatin receptor 1–5; Tacr1–3, tachykinin receptor 1–3; Th, tyrosine hydroxylase.
2.3. Anatomical Experiments
An additional set of animals (n = 6/group) was used for vesicular acetylcholine transporter (vAChT) immunohistochemistry. Animals received the same pharmacological treatment for 6.5 days as described above. Experimental groups were matched and individuals from different groups were processed in parallel. Animals were anesthetized with 100 mg/kg ip sodium pentobarbital and transcardially perfused through ascending aorta with 50 ml of NS, followed by 250 ml of 4% paraformaldehyde in 0.1M phosphate buffer (PB, pH 7.4, room temperature) on the morning of day 7. Brains were removed and stored in the same fixative solution overnight at 4°C before cryoprotection in 30% sucrose solution in 0.1M PB for at least 48 hours. Subsequently, brains were frozen and 40-micron coronal sections were cut on a freezing microtome (Leica Microsystems, Wetzlar, Germany). Free-floating sections were collected in 0.1M PB in saline and subsequently processed for vAChT immunohistochemistry. Primary (goat anti vAChT, 1:1000 dilution; Cat# ABN100; EMD Millipore, Billerica, MA) and secondary antisera (Cy3 conjugated anti-goat, 1:200 dilution; red fluorophore; Jackson ImmunoResearch Labs, Inc., West Grove, PA) were diluted in 0.1M PB with NS, 0.3% Triton X-100, 0.04% bovine serum albumin, and 0.1% sodium azide. Brain sections were incubated in primary antibodies for 2 days at 4°C, and subsequently in secondary antibodies for 1 to 2 hours at room temperature. Sections were rinsed in 0.1M PB in saline (3 times for 10 minutes) in between immunocytochemical processing. Finally, sections were rinsed in 0.1M PB in saline solution prior to mounting on slides in 0.05M PB. After drying, mounted sections were coverslipped with 90% glycerol solution. We used fluorescent/bright-field microscope (Olympus IX81; Olympus America Inc., Melville, NY, USA) equipped with a camera and digital microscopy software used for the analysis (Slidebook v4.2; Olympus). Immunolabeling with anti-vAChT antisera produced characteristic staining of the neuronal cytoplasm (Fig. 2A and B). Note that complementary sets of brain tissue were immuno-processed simultaneously and photographed with the same exposure time to minimize inter-assay variability. Specifically, bilateral pictures of the LDTg were taken uniformly with 10x magnification lens at 400 ms exposure for all brains. Average intensity labeling of vAChT immunohistochemistry per individual neuron was calculated based on analysis of intensity labeling of 5 individual neurons per picture of LDTg (total 6 pictures/brain, n = 6 brains/treatment group). Background labeling intensity (non-labeled neuropil) in each picture was subtracted from total intensity of vAChT labeling of each individual neuron that was analyzed. An observer blinded to the treatment group performed the analysis.
Photomicrographs illustrate vesicular acetylcholine transporter (vAChT) immunofluorescence in the laterodorsal tegmental nucleus. Cholinergic neurons are labeled more intensely following chronic morphine treatment (B) in comparison to saline control group (A). Graph in Panel C illustrates average percentage (%) intensity of vAChT immunoreactivity per individual cholinergic neuron in the laterodorsal tegmental nucleus (± SD; n = 6/group). There is a statistically significant increase (*) in intensity of vAChT immulabeling/neuron following chronic morphine administration when compared to control (99% ± 43.31; t(10) = -5.61, two-tailed p<0.001). Scale bar = 100 μm.
2.4. Behavioral Analysis
Additional sets of animals underwent behavioral experiments following 6.5 days of treatment with different drugs (saline sc, morphine (10mg/kg sc), and/or mecamylamine (0.5 or 2 mg/kg via intraperitoneal (ip) route; Cat#M9020; Sigma-Aldrich, St. Louis, MO). Mecamylamine is widely used as a broad-spectrum noncompetitive antagonist of neuronal nicotinic acetylcholine receptors [33]. It is clinically applicable ganglionic blocker that crosses blood-brain barrier. Mecamylamine was administered either alone or immediately following morphine administration as a separate injection. All injections were done in the home cage. Antinociceptive effects of morphine were tested using the Hot Plate test in the afternoon of day 7. To minimize the number of animals used, as well as to optimize the data collection, additional set of animals underwent locomotor recordings one hour after the last injection on day 7 followed by recordings of precipitated withdrawal as described below.
2.4.1. Hot Plate Test. We analyzed 4 pharmacological groups that received twice-daily sc injections for 6.5 days: (1) NS control group (n = 6), (2) chronic morphine group (10 mg/kg; n = 6), (3) chronic mecamylamine group (2 mg/kg; n = 4), and (4) chronic morphine (10 mg/kg) and mecamylamine (2 mg/kg) group (n = 5). Since repeated analgesic testing exaggerates the magnitude of antinociceptive tolerance in adult rats [34–36], we performed antinociceptive tolerance assessments using the Hot-Plate Latency Test only in the afternoon of day 7. After adaptation baseline trials, and trials following injection of NS (equivalent volume to 10 mg/kg morphine) each rat was injected with a low dose of morphine (0.1 mg/kg sc). Thirty minutes later, the rat was re-tested and injected with the next dose of morphine that was increased in a logarithmic manner using a dose range of 0.1–10 mg/kg, and increments of approximately half a log unit each time (such that each animal received 0.1, 0.3, 1, 3, and 10 mg/kg of morphine sequentially) as described before [26,29]. We used a modified Hot Plate test [37] (Temperature: 56°C and 12 s cutoff latency) to measure distal 2/3 hindpaw withdrawal latency in seconds. Testing began 20 minutes following the last administration of the drug. The withdrawal latency of the hindpaw of each animal was measured 3 times on both sides after each drug injection (with 10 s pause interval) and the final withdrawal latency value was averaged among 6 recordings. In none of these cases was any tissue damaged. An individual blinded to the treatment group did the behavioral testing. Hot Plate test data in the form of cumulative morphine dose-response curves are presented as a percentage of maximum possible effect (%MPE = (Test Latency—Baseline Latency/Cutoff Time—Baseline Latency) x100) ± SD according to the method of Harris and Pierson [38].
2.4.2. Locomotor Activity Assay. Drug sensitization is defined as an increased effect of drug following repeated doses (the opposite of drug tolerance). Classic study of sensitization relate to ‘incentive salience’, and the rewarding, addictive qualities of drugs of abuse [39]. However, there is also sensitization to the locomotor activating properties morphine, and these can be demonstrated as a consequence of repeated exposure without an intervening period of abstinence [40]. Our study of locomotor activity evaluation included another set of pharmacological groups that received twice-daily sc injections for 6.5 days: (1) saline control (n = 9), (2) acute morphine group (saline sc for 6 days and only morphine (10mg/kg sc) on the morning of day 7; n = 9), (3) mecamylamine control (2 mg/kg twice-daily for 6.5 days; n = 5), (4) chronic morphine group (10 mg/kg; n = 11). Mecamylamine effect on expression of locomotor activation was tested with (5) chronic morphine + acute mecamylamine group (morphine for 6 days and both morphine and mecamylamine (0.5 mg/kg or 2 mg/kg) on the morning of day 7; n = 7/group). Finally, mecamylamine effect on development of locomotor activation was evaluated by (6) chronic morphine + chronic mecamylamine group (morphine and mecamylamine (0.5 mg/kg or 2 mg/kg) for 6 days and only morphine on the morning of day 7; n = 7/group). All injections were done in the home cage. One hour after the last injection on day 7 (end of 6.5 days of pharmacological treatment), locomotor activity was measured in clear plexiglass cages (10”x19”x8”h; Photobeam Activity System, San Diego Instruments, San Diego, CA) over twelve 5-min intervals for 60 minutes. Analysis excluded the first 5-min interval to minimize differences in locomotor activity during accommodation to a new environment. Total locomotor activity data (ambulatory, fine, and rearing movements) are expressed as mean ± SEM per pharmacological group.
2.4.3. Morphine Withdrawal Paradigm. Physical dependence is manifested indirectly as a myriad of physiological disturbances and physical symptoms of withdrawal that result from abrupt morphine discontinuation or dosage reduction (spontaneous withdrawal), or as a result of opioid antagonist administration (precipitated withdrawal). The following pharmacological groups included in locomotor analysis were also used for the withdrawal analysis: (1) saline control (n = 10), (2) chronic morphine group (10 mg/kg; n = 5), (3) chronic morphine + acute mecamylamine group (0.5 mg/kg; n = 7 or 2 mg/kg; n = 8), and (4) chronic morphine + chronic mecamylamine group (0.5 mg/kg or 2 mg/kg; n = 7/group). Precipitated withdrawal was induced by a single injection of an opioid receptor antagonist, naloxone (20 mg/kg ip; N7758, Sigma-Aldrich Co., St. Louis, MO) immediately after completion of locomotor testing (2 hours after the last morphine and/or mecamylamine injections). The withdrawal was videotaped for 30 minutes. Recorded videos were analyzed and 13 behaviors were scored every 15 seconds according to the previous studies [41] by an observer blind to the treatment group. Briefly, 13 scored withdrawal behaviors corresponded to 2 different types: ‘checked signs’ and ‘graded signs’. The former are behaviors for which only the absence or presence of the behavior is evaluated and includes facial fasciculation/teeth chattering, profuse salivation, abnormal posture, erection/ejaculation, ptosis, chromodacryorrhea, and irritability. “Graded signs” are scored based on the frequency (# of events) and include escape attempts, abdominal constrictions/writhing, “wet dog” shakes, rearing, grooming, and diarrhea (# fecal boli). Modifications of the Gellert-Holtzmann method included the elimination of two behaviors (weight loss and swallowing movements), and the addition of two graded behaviors (rearing and grooming). Other modification included scoring diarrhea (#fecal boli) as a graded sign. Note that naloxone-induced withdrawal hyperalgesia (decrease in nociceptive threshold), which also refers to expression of dependence, was not performed. Withdrawal scores were expressed as individual sign means ± SD as well as the sum of all behavioral scores for each animal (total global score mean ± SD) for the 30 minutes of precipitated withdrawal.
2.5. Data Analysis
For gene expression analysis and average vAChT intensity labeling we used two-tailed Student’s t-test. For the Hot Plate test, locomotor testing, and morphine withdrawal behavioral assays, we used a one- or two-way analysis of variance (ANOVA) followed by either Tukey or LSD post-hoc test. Significance was established at p<0.05. All statistics were performed using IBM SPSS Statistical Software.
Results
3.1. Gene expression changes in the periaqueductal gray following chronic morphine
We performed chronic opioid administration study to determine the counteradaptive molecular mechanisms that underlie neural plasticity induced by chronic morphine exposure. To determine how chronic morphine administration alters gene expression in the PAG, we analyzed gene expression differences following chronic morphine treatment in comparison to saline control using Neurotransmitters and Receptors PCR array. Area of analysis included ventral and ventrolateral PAG at the level of the inferior colliculus where serotonin neurons comprise dorsal raphe nucleus and cholinergic neurons comprise LDTg (Fig. 1). Molecular analysis showed that out of a total of 84 genes surveyed, only 8 genes exhibited expression changes that were either significant (p<0.05) or had more than 2-fold change (100%) but were statistically marginally non-significant (p = 0.05–0.2) (Table 1). Specifically, we report significant increase in the expression of acetylcholine esterase (AChe), gene encoding the enzyme responsible for the acetylcholine degradation (7.98 fold; t(4) = -2.4; p = 0.03), as well as significant decrease in gene expression following chronic morphine administration for melanocortin 2 receptor (Mc2r; -2.9 fold; t(4) = 5.77; p<0.01), GABAergic receptor subunit rho-1 (Gabrr1; -2.7 fold; t(5) = 2.02; p = 0.04), and cholinergic nicotinic alpha 3 receptor subunit (Chrna3; -2.5 fold, t(5) = 2.25; p = 0.03). Additional changes included a large fold increase with marginal non-significance in expression of genes encoding for GABA-A receptor, subunit alpha 6 (Gabra6; 7.4 fold, t(5) = -1.08; p = 0.16) and that of choline acetyltransferase (Chat; 4.4 fold; t(6) = -0.86; p = 0.2), the gene encoding the enzyme that synthesizes acetylcholine. Similarly, we also report a large fold decrease with marginal non-significance in gene expression of cholinergic nicotinic beta 4 receptor subunit (Chrnb4; -6.48 fold, t(5) = 1.66; p = 0.08) and dopamine receptor D4 (Drd4; -3.9 fold, t(6) = 1.33; p = 0.12). In contrast, none of the genes related to glutamatergic, noradrenergic, serotonergic, cholecystokinin, somatostatin, galanin, or glycine neurotransmission were expressed differently following chronic morphine treatment in adult rats.
3.2 Chronic morphine increases the expression of the cholinergic marker, vAChT, in the Laterodorsal Tegmental Nucleus
Our molecular data suggested that the cholinergic system in the region of analysis (Fig. 1) might be affected by chronic morphine administration. Specifically, 2 genes were significantly different (upregulation of AChe and downregulation of Chrna3), while 2 other genes showed a large magnitude of difference, but only a trend toward significance (upregulation of Chat, and downregulation of Chrnb4). Our next aim was to quantify protein expression of the cholinergic marker vAChT at the level of the LDTg using a neuroanatomical approach. Specifically, we measured % intensity of vAChT fluorescent immunoreactivity per individual cholinergic neuron of the LDTg (Fig. 2). Chronic morphine treatment led to a significant increase of about 100% in vAChT immunoreactivity/neuron following chronic morphine administration when compared to control values (99% ± 43.31; t(10) = -5.61, p<0.001). This result provides additional anatomical evidence of cholinergic system alterations in the LDTg after chronic morphine administration.
3.3. Behavioral implications of the cholinergic system in chronic morphine effects
Our next goal was to test a possible functional role of transcriptional adaptations found in the cholinergic system upon different aspects of chronic morphine exposure. Our general working hypothesis for behavioral experiments is that nicotinic receptors are involved in chronic morphine-induced neural and behavioral plasticity. Specifically, we evaluated the effects of the selective neuronal nicotinic receptor antagonist mecamylamine on three negative behavioral aspects involved in chronic morphine treatment: antinociceptive tolerance, locomotor sensitization, and opioid dependence.
3.3.1. Antinociceptive Tolerance. To evaluate the development of antinociceptive tolerance in the presence of mecamylamine (2 mg/kg), we used the Hot Plate test (Fig. 3). Chronic mecamylamine administration did not change antinociceptive effects of acute morphine. In addition, chronic administration of mecamylamine together with morphine did not have any effect on development of antinociceptive tolerance (Fig. 3). Specifically, two-way ANOVA shows a significant effect of morphine (F(1,17) = 377.69, p<0.0001 at 10 mg/kg testing dose), while there is no significant effect of mecamylamine nor interaction effect. These findings indicate that the nicotinic cholinergic system would not be involved in the development of antinociceptive tolerance induced by chronic morphine administration.
Hot Plate test was done in the afternoon on day 7 of treatment to evaluate development of antinociceptive tolerance. Chronic morphine administration (10 mg/kg sc twice-daily for 6.5 days; n = 6) was associated with development of antinociceptive tolerance in comparison to saline control group (n = 6). Chronic mecamylamine (Mec) administration (2 mg/kg sc twice-daily for 6.5 days; n = 4) did not change morphine antinociceptive effect. In addition, when given chronically with morphine, mecamylamine did not change development of antinociceptive tolerance (n = 5). Results are presented as a percentage of maximum possible effect (%MPE ± SD) according to the method of Harris and Pierson [38] to construct dose-response curves for morphine’s antinociceptive effect. Two-way ANOVA shows a significant effect of morphine (F(1,17) = 377.69, p<0.0001 at 10 mg/kg testing dose), while there is no significant effect of mecamylamine nor interaction effect. **, p<0.01.
3.3.2. Locomotor Sensitization. We confirmed that following chronic morphine administration locomotor activity in rats increased in comparison to saline control and acute morphine treatment (Fig. 4A; F(3,30) = 26.05, p<0.001) validating our locomotor sensitization model. Furthermore, chronic mecamylamine administration alone had no effect on locomotor activity. In contrast, an acute dose of mecamylamine (0.5 mg/kg) on the day of testing (day 7 of morphine treatment) attenuated the expression of locomotor sensitization induced by morphine. Moreover, a higher dose of the nicotinic blocker (2 mg/kg) fully blocked the sensitizing effects of chronic morphine on locomotor activity (Fig. 4B; F(3,30) = 15.21, p<0.001). When we examined the effects of chronic mecamylamine on the development of locomotor sensitization induced by morphine, we found a much smaller effect of the nicotinic antagonist, only significant for the high dose tested (2 mg/kg) in comparison to chronic morphine animals (Fig. 4C; F(3,30) = 12.37, P<0.001). This chronic mecamylamine treatment did not fully reverse locomotor sensitization induced by morphine (Fig. 4C). Presented findings indicate that the nicotinic cholinergic system could contribute both to expression and development of locomotor sensitization induced by chronic morphine. Endogenous nicotinic transmission, however, would have a more important role in the expression than in the development phase of morphine’s sensitizing effects.
Graphs illustrate average total locomotor activity (ambulatory, fine, and rearing movements) ± SEM. (A) Chronic morphine administration (10 mg/kg sc twice-daily for 6.5 days; n = 11) was associated with locomotor sensitization when measured on day 7. It was significantly different (F(3,30) = 26.05, p<0.001) in comparison to saline control (n = 9; p<0.001), acute morphine group (saline sc twice-daily for 6 days and morphine 10 mg/kg sc in the morning on day 7; n = 9; p<0.001), and chronic mecamylamine (Mec) administration (2 mg/kg sc twice-daily for 6.5 days; n = 5; p<0.001). Panel B illustrates acute Mec effect on expression of locomotor activation (F(3,30) = 15.21, p<0.001). Mec was administered in a single dose on day 7 (0.5 or 2 mg/kg dose) to animals that were chronically treated with morphine. Although 0.5 mg/kg acute Mec dose (n = 7) statistically decreased locomotor sensitization associated with chronic morphine administration (p = 0.036), it was the 2 mg/kg dose (n = 7; p<0.001) that decreased it to the saline control level. Panel C illustrates chronic Mec effect on development of locomotor sensitization (F(3,30) = 12.37, P<0.001). Mec was administered twice daily in 0.5 or 2 mg/kg dose along morphine for 6 days. Prior to the locomotor testing in the morning of day 7, animals received only morphine. Smaller Mec dose (n = 7) had no effect, while 2 mg/kg chronic Mec administration (n = 7) significantly decreased development of locomotor sensitization in comparison to the chronic morphine group (p>0.006). However, it was still significantly higher in comparison to saline control (p<0.02). Data for saline control and chronic morphine group is the same in A–C. One-way ANOVA with LSD post-hoc test; *, statistically different from all other groups; #, statistical difference only between marked groups.
3.3.3. Opioid Dependence. In order to further evaluate the specificity of mecamylamine’s effect on locomotor sensitization, we also evaluated physical dependence following chronic morphine administration by studying withdrawal. We induced precipitated withdrawal by administering an acute injection of the opioid antagonist naloxone following the last morphine injection on day 7. After a 30 min observation session, animals were scored for global withdrawal symptoms. Global withdrawal score means [41,42] were significantly higher in all groups that received chronic morphine (F(5,38) = 22.40; p<0.001). However, administration of either acute or chronic mecamylamine (0.5 or 2 mg/kg dose) had no effect on withdrawal (Fig. 5A). There were no differences in the means of individual behaviors among groups (not shown), except for the average number of fecal boli (Fig. 5B; F(5,38) = 41.068, p<0.001). Specifically, only 2 mg/kg acute dose of mecamylamine significantly decreased average number of fecal boli following chronic morphine administration (p<0.001) in comparison to other groups with chronic morphine administration (Fig. 5B). Although average number of fecal boli is significantly decreased following 0.5 mg/kg acute dose of Mec in comparison to its chronic application (p<0.003), it was still not different from chronic morphine group (Fig. 5B). The results show that of all the graded behaviors (weight loss, escape attempts, abdominal contractions, wet dog shakes) and checked behaviors (facial fasciculation’s or teeth chattering, swallowing movements, profuse salivation, ptosis, abnormal posture), only diarrhea (# fecal boli) was attenuated in a dose-dependent manner by an acute dose of mecamylamine.
Panel A graph illustrates average global withdrawal score ± SD. It is significantly higher (F(5,38) = 22.40; p<0.001) following chronic morphine administration (n = 5) in comparison to saline control group (n = 10). However, it was not different from either acute mecamylamine (Mec) (n = 7 for 0.5 mg/kg dose; n = 8 for 2 mg/kg dose group), or chronic Mec groups (n = 7/group). (B) There were no differences in average number of individual behaviors among groups (not shown), except for the average number of fecal boli (#fecal boli/animal/30 min withdrawal ± SD; F(5,38) = 41.068, p<0.001). Only 2 mg/kg acute dose of Mec significantly decreased average diarrhea following chronic morphine administration (p<0.001) to a saline control level. Although average number of fecal boli is significantly decreased following 0.5 mg/kg acute dose of Mec in comparison to its chronic application (p<0.003), it was still not different from chronic MSO4 group. One-way ANOVA with LSD post-hoc test. *, statistically different from all other groups except each other; #, statistical difference only between marked groups.
Discussion
In the present study, molecular analysis was done in conjunction with anatomical and behavioral studies to identify candidate neurotransmitters in the ventrolateral and ventral PAG affected by systemic chronic morphine administration. We report gene expression changes, as well as vAChT immunoreactivity changes possibly associated with an increased metabolic turnover of the cholinergic system in the area of interest, the LDTg. Furthermore, our behavioral findings support a selective role of endogenous nicotinic cholinergic neurotransmission in the expression and development of sensitizing effects of chronic morphine. On the other hand, disruption of the nicotinic cholinergic neurotransmission did not affect antinociceptive tolerance or dependence to morphine. Behavioral results indicate a clear dissociation of behavioral effects mediated by nicotinic cholinergic system following chronic morphine administration.
4.1. Gene Expression Changes in the Ventral and Ventrolateral PAG Following Chronic Morphine Administration
PCR is a sensitive method for detection of RNA expression levels in selected tissue. We used a pooling strategy combined with biological and technical replication to optimize this approach. In particular, pooling was used to minimize individual variation as well as technical variation caused by the dissections themselves (n = 5 rats/replicate/group). We minimized technical variation by dissecting the region of interest specifically at the level of the inferior colliculus. While our statistical approach was subject to false-positive error, relatively few differences in gene expression were detected related to treatment (morphine vs. NS control). Our study looked into the gene expression changes related to the chronic morphine paradigm. We cannot rule out that some gene transcriptional effects may not require chronic exposure. Interestingly, despite the neurochemical diversity of the region of interest, out of a total of 84 genes related to different neurotransmitter receptors and regulators, we report statistically significant changes in 4 genes (up-regulation of AChe, and down-regulation of Mc2r, Gabrr1, and Chrna3), and a large fold increase with marginal non-significance in expression of 4 additional genes (up-regulation of Gabra6 and Chat; down-regulation of Chrnb4 and Drd4). According to a genome-wide, high-resolution atlas of gene expression throughout the adult mouse brain (Allen Brain Atlas[43]), in situ hybridization analyses showed baseline patterned expression of mRNA of all genes (with exception of no data for Chrnb4) within the ventral and ventrolateral PAG, with Chat selectively expressed within neurons corresponding to anatomical location of cholinergic neurons comprising the LDTg [44,45]. It was also reported that alpha 7 (not included in the array) and beta 2 nicotinic acetylcholine receptor mRNAs are also co-expressed in almost all rat cholinergic cells including those of mesopontine tegmentum [46]. However, high-throughput studies that looked into potential gene networks associated with chronic morphine administration are limited. A gene expression profiling and pattern matching study in different mouse strains by Tapocik et al. [28], identified 64 genotype signature genes at the PAG that are postulated to predispose animals to morphine induced tolerance. Indeed, one of these ‘predisposition genes’ at the level of the PAG was Ache. These authors also reported 81 genes (positive and negative) at the PAG that are postulated to represent potential mediators of analgesic tolerance whose expression is modulated by chronic morphine administration. Three of these ‘tolerant genes’ that included Gad1 (glutamate decarboxylase 67 kDa isoform), Glrb (glycine receptor, beta subunit), and Cnr1 (cannabinoid receptor 1, brain) showed up-regulations following chronic morphine treatment. None of the genes related to glutamatergic or glycine neurotransmission were detected in our study in rats. Neither our nor study by Tapocik et al. [28] reported gene changes related to noradrenergic, serotonergic, cholecystokinin, somatostatin, or galanin neurotransmission following chronic morphine treatment in rats or mice respectively. Methodological differences (PCR Array vs. microarray gene expression profiling), different animal species (rat vs. mouse), areas included in dissected tissue (ventral and ventrolateral PAG vs. PAG), and/or pharmacological treatment schedules (6 ½ twice-daily sc injections vs. three or five sc injections/3 days) could explain differences between their study and ours. Finally, future studies should include quantitative rtPCR of genes present in cholinergic neurons of the LDTg: Chat that showed large fold increase with marginal non-significance following chronic morphine administration, and genes that were not included in the array (e.g. vAchT; high-affinity choline transported (ChT); cholinergic nicotinic receptor, alpha 7 (Chrna7)).
4.2. Neuroplasticity of the Cholinergic Neurotransmission in the LDTg Following Chronic Morphine Administration
Since our molecular data raised the possibility of increased cholinergic neurotransmission following chronic morphine administration at the level of the ventral and ventrolateral PAG, we extended PCR findings by analyzing protein expression levels of a specific cholinergic marker within neurons of the LTDg using a quantitative immunofluorescent technique. Although widely used for the last 50 years [47–49], histochemical technique to visualize acetylcholinesterase [50] was difficult to use as a quantitative measurement in the area of LDTg (not shown) because of its primary association with cholinergic terminals rather than cell bodies. Instead, we used VAChT, a proton-dependent transporter that facilitates packaging of acetylcholine into synaptic vesicles, as a useful marker for identifying both cholinergic neurons and terminals [51]. Our data show significant quantitative increase in vAChT immunolabeling in LDTg neurons at the ventrolateral PAG following administration of chronic morphine in comparison to saline control. These anatomical data support the hypothesis of an increased turnover of the cholinergic transmission following chronic morphine administration. Future studies should include quantification of LDTg protein expression with chronic morphine administration using a Western Blot technique to further support presented findings.
Cholinergic neurons of LDTg/pedunculopontine tegmentum are known to provide the only cholinergic input to the VTA [14,16,17]. Specifically, cholinergic neurons of the LDTg project mainly to the VTA [14] where they make excitatory synapses on dopaminergic neurons, which in turn project to the nucleus accumbens [15]. In fact, a major portion of cholinergic input to VTA likely involves an excitatory influence on dopaminergic mesoaccumbens neurons [52]. Furthermore, electrical stimulation of the LDTg promotes burst firing of VTA dopaminergic neurons and increases dopamine release in the nucleus accumbens [10,11]. Therefore, cholinergic neurons originating from LDTg have a pivotal role in the modulation of motivated behavior, reward pathways, and addiction (see reviews [27,53]). Demonstrated cholinergic neuroplasticity as shown by our anatomical data, could in part be implicated in the mediation of reward and addiction processes following chronic morphine administration.
4.3. Cholinergic Nicotinic Role in Behavioral Effects of Chronic Morphine
Presented behavioral studies confirmed that chronic morphine administration in rats [26,29] is associated with negative behavioral effects: antinociceptive tolerance (Fig. 3), locomotor sensitization (Fig. 4A), as well as opioid dependence (Fig. 5A) (see review [54]). There is a well-known dissociation in the mechanisms that underlie each of these behavioral aspects associated with chronic morphine exposure. Certainly, it is long known that the central cholinergic system is, in part, involved in opiate-induced effects [55]. Our results indicate that mecamylamine, nicotinic receptor antagonist, inhibits the neural plasticity underlying morphine locomotor sensitization but not morphine tolerance or physical dependence. Results are suggestive that nicotinic receptors are involved in mediation of specific chronic morphine induced changes in behavior, locomotor sensitization.
4.3.1. Locomotor Sensitization to Morphine. Behavioral findings support a selective role of nicotinic cholinergic neurotransmission in the expression, more than the development of sensitizing effects of chronic morphine, without affecting antinociceptive tolerance or dependence to morphine. Locomotor sensitization has been proposed to correspond to certain aspects of drug addiction such as compulsive drug-seeking behavior, and is associated with activation of the mesolimbic dopamine system (see above) [39,56,57]. This may reflect learning, however, increased locomotor response that is conditioned or context dependent is only observed if drugs of interest were administered in an environment that is similar or same to the test environment [58]. Considering that we used unpaired paradigm (animals tested in an environment that differed (unpaired) from that used for injection), recorded locomotor activity is more likely context-independent sensitization [58]. Traditionally, development of sensitization to drugs of abuse is commonly linked to the VTA, while expression of sensitization is associated with the nucleus accumbens [59]. In addition to the mesocorticolimbic system (i.e., VTA, nucleus accumbens, and medial prefrontal cortex), the ventral pallidum, hippocampus, amygdala, LDTg, and the paraventricular nucleus of the thalamus have all been suggested to play a role in the development of sensitization to drugs of abuse. We report that an acute dose of mecamylamine on the day of testing attenuated the expression, while chronic mecamylamine administration to a smaller degree decreased the development of locomotor sensitization induced by chronic morphine. These findings are supportive of different cholinergic mechanism on development and expression of behavioral sensitization to morphine (see review [59]). These results are also highly suggestive of distinct sensitivity of the involved neural networks to cholinergic modulation.
VTA was demonstrated to be critical for behavioral activation/sensitization by morphine [60–63]. It was demonstrated that nicotinic acetylcholine receptors regulate the neuronal activity in the VTA [64,65], and critically mediate the rewarding effects of morphine [66]. Activation of nicotinic acetylcholine receptors (nAChRs) in the VTA excites dopamine neurons via somatic receptors and by modulating both excitatory and inhibitory inputs [64,65,67–69]. Indeed, dopamine release in the nucleus accumbens is modulated by cholinergic activation of the VTA from brainstem cholinergic nuclei [70]. The high levels of nAChR expression in the VTA [68], along with the strong endogenous cholinergic input from the brainstem, implicated LDTg as an important cholinergic modulator of midbrain dopaminergic systems [24,27,71]. However, other brain regions with either cholinergic cells or receiving cholinergic innervation show changes in neuronal activity following morphine exposure. Morphine-induced changes in giant cholinergic interneurons in rat nucleus accumbens and striatum may serve as a model for postsynaptic plasticity involved in the long-term effects of drugs of abuse. Specifically, presynaptic opioid receptors directly modulate dopamine release in the striatum [72,73], which in turn tonically inhibits striatal cholinergic interneurons [74]. Furthermore, chronic morphine administration leads to a long-lasting augmentation of the electrically evoked acetylcholine release in slices of both nucleus accumbens and striatum [75]. Such results implicate cholinergic neurotransmission of the forebrain in the long-lasting psychomotor sensitization and enhanced vulnerability towards the acquisition of drug addiction (see review [39]). In contrast, work by Nakashini’s group investigated the role of nucleus accumbens cholinergic circuit by selectively ablating the cholinergic interneurons within the nucleus accumbens with the use of immunotoxin-mediated cell targeting techniques [76,77]. Authors reported that acetylcholine regulates the nucleus accumbens circuit jointly with dopamine but in the opposite fashion by preventing long-lasting behavioral changes of cocaine [76,77] and morphine addiction [78]. In other words, enhanced cholinergic neurotransmission in the nucleus accumbens inhibits morphine addiction. Taken together, reported opposing effects of cholinergic system on morphine addiction are possibly related to different brain areas. Future pharmacological studies (e.g. stereotaxic injections of mecamylamine directly into the VTA or selective ablation of the cholinergic neurons of the LDTg) should elucidate whether nicotinic cholinergic mechanisms that are responsible for locomotor sensitization following chronic morphine administration are mediated by cholinergic neurons originating from LDTg (as implicated by our molecular and anatomical data), as opposed to cholinergic neurons of the forebrain. In addition, these future studies might elucidate cholinergic role in development versus expression of locomotor sensitization to chronic morphine administration.
4.3.2. Antinociceptive Tolerance. Our behavioral data implicate that cholinergic nicotinic neurotransmission would not be involved in neither the antinociceptive effect of acute morphine nor the development of antinociceptive tolerance to morphine. Previous studies demonstrated that acetylcholine is involved in the manifestation of analgesia [79], as well as acute morphine effects, but had no direct role in development of morphine antinociceptive tolerance [80,81]. Although mecamylamine in the mesolimbic system antagonizes analgesia with lower doses of systemically administered morphine (5 mg/kg), this effect is “washed out” at higher doses (10 mg/kg) [82], in agreement with our findings. Together with presented molecular findings, data suggest that neuroplasticity of the cholinergic system at the level of ventral and ventrolateral PAG does not affect (1) the descending pain modulatory pathways known to be involved morphine-mediated modulation of nociception [22,83,84], or neurocircuitry critical for development of antinociceptive tolerance to morphine [20–22,85–88].
4.3.3. Opioid Dependence. Morphine dependence and withdrawal have been associated with alterations in cholinergic signaling pathways [89–91]. Our data demonstrate that chronic mecamylamine does not alter withdrawal syndrome in adult rats chronically treated with morphine. Although we did not differentiate between development and expression of opioid dependence, co-treatment of mecamylamine with chronic morphine exposure appeared to have no effect on withdrawal. These results indicate a lack of nicotinic effects in mediating morphine’s dependent state. The only individual sign that showed attenuation with acute mecamylamine administration at 2 mg/kg dose was diarrhea. Similarly to our findings, a previous study by Taraschenko et al. [92] showed that administration of mecamylamine attenuated diarrhea during withdrawal to morphine. Locus coeruleus is involved in behavioral and neurochemical changes associated with naloxone-precipitated withdrawal [93,94]. In the absence of attenuation of any other withdrawal symptoms by mecamylamine, it is highly unlikely that the neuronal nicotinic acetylcholine receptors [33] at the locus coeruleus mediate expression of diarrhea. More probable explanation is that mecamylamine reverses fecal boli excretion by blockade of densely expressed nicotinic receptors in the myenteric neurons of the gut [95] that critically regulate peristalsis [96]. In contrast to our findings, Taraschenko et al. [92] also reported attenuation in rearing and teeth chattering. Differences between their study and ours could be related to animal gender (female vs. male), or differences in morphine dosing schedule (incremental dosing [97] vs. single dose over 7 days). Finally, a recent study showed that genetic blockade of cholinergic nicotinic transmission in the medial habenula nucleus does not modify the effects of cholinergic drugs on morphine’s withdrawal [98]. Similar analysis should investigate specific administration of mecamylamine effect in the VTA. Future studies should also elucidate if mecamylamine could have a selective role in the treatment of somatic sigs of withdrawal to opioid drugs of abuse [99].
Conclusions
Our findings contribute to a better understanding of the gene expression changes underlying systemic chronic action of morphine at the level of the PAG. Our behavioral findings support a selective role of endogenous nicotinic cholinergic neurotransmission in expression and development of locomotor sensitization to chronic morphine. Future studies should elucidate changes in cholinergic neurotransmission at the ventrolateral PAG with the selective role of LDTg in locomotor sensitization to morphine, as well as therapeutic potential of drugs acting on the nicotinic receptors in the co-treatment of addiction.
Acknowledgments
Presented work was supported by Foundation for Anesthesia Education and Research (FAER), Endo Pharmaceuticals, and NIH R03 DA030874 (DB), Mayo Family Research Endowment Fund (CBB), and NIH R01 DA021801 (KGC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. In addition, funding from Endo Pharmaceuticals does not alter our adherence to PLoS ONE policies on sharing data and materials. Authors would also like to thank Dr. Jia-Ren Liu for technical expertise in molecular techniques, and Dr. Sulpicio G. Soriano for the use of the equipment for the total RNA extraction.
Author Contributions
Conceived and designed the experiments: DB CBB KGC. Performed the experiments: DB MS-R ALS. Analyzed the data: DB MS-R ALS KGC. Contributed reagents/materials/analysis tools: DB MS-R CBB KGC. Wrote the paper: DB MS-R ALS CBB KGC.
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