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Correction: Genetic and Metabolomic Dissection of the Ergothioneine and Selenoneine Biosynthetic Pathway in the Fission Yeast, S. pombe, and Construction of an Overproduction System

  • The PLOS ONE Staff

Correction: Genetic and Metabolomic Dissection of the Ergothioneine and Selenoneine Biosynthetic Pathway in the Fission Yeast, S. pombe, and Construction of an Overproduction System

  • The PLOS ONE Staff
PLOS
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There are formatting errors in Tables 1, 3, and 4 of the published article. The correct tables can be viewed here.

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Table 1. Normalized peak areas of the four compounds composing the EGT biosynthetic pathway obtained by metabolomic analysis of WT and newly constructed strains. Values were measured from metabolome samples of three different S. pombe strains in three different cultivation conditions, as indicated. Mass values (m/z) and LC retention times (min) of each peak are included for reference.

https://doi.org/10.1371/journal.pone.0097774.t001

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Table 3. Absolute intracellular EGT concentrations (µM) in S. pombe cells. Intracellular concentrations were derived from measured normalized peak areas using a calibration curve generated by injections of pure EGT in 10-fold dilution steps. The detailed calculation method is described in Figure S6.

https://doi.org/10.1371/journal.pone.0097774.t003

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Table 4. S. pombe strains used in this manuscript. Strains from the Bioneer haploid deletion mutant collection [24] were backcrossed with WT 972 to remove auxotrophic markers.

https://doi.org/10.1371/journal.pone.0097774.t004

Reference

  1. 1. Pluskal T, Ueno M, Yanagida M (2014) Genetic and Metabolomic Dissection of the Ergothioneine and Selenoneine Biosynthetic Pathway in the Fission Yeast, S. pombe, and Construction of an Overproduction System. PLoS ONE 9(5): e97774