Geographical distribution of eIF4E variants

A total of 2803 pea accessions were screened for eIF4E intron 3 length polymorphism using two sets of primer combinations, resulting in the detection of four respective eIF4E^A-B-C-S variants (Table 1, Fig. 1). The first corresponded to the already known eIF4E^A resistance [31] variant (amplified 243 bp fragment with primer combination A and 536 bp with primer combination B), the second to the susceptible eIF4E^S [31] (293 and 586 bp fragments), the third to the novel eIF4E^B (293 and 536 bp fragments) and the fourth to the novel eIF4E^C (293 and 592 bp fragments). The resistance eIF4E^A variant was found in seven accessions from Ethiopia-Sudan, fourteen accessions from India, five accessions from Pakistan-Nepal and one accession each from China, Russia and Afghanistan. In addition, this variant was detected in fifteen USA and Canadian, two South American and eleven European modern varieties or breeding lines, which have the Ethiopian line PI193835 in their pedigree. In total, the eIF4E^A variant was detected in 53 accessions, which represent 1.9% of those tested. The eIF4E^B variant was abundant in accessions of Ethiopian (29%), Afghan (23%), Nepal-Indian (20%), Caucasus (6.4%) and particularly Chinese (36.5%) origin (Table 2, Fig. 2). Moreover, it was found in five (e.g. 20%) accessions of South American origin. In contrast, this variant was under-represented in landraces of European origin, except in the Balkans (4 acc.), and absent from modern pea varieties. The eIF4E^C variant was the most frequent in Iberian peninsula (25%) and India-Nepal (16.6%), followed by Balkan (9%), Ethiopia (6%), and Afghan (6.2%) locations, and occurring in total of 91 (3.2%) accessions (Table 2). Due to bulking of 10 plants per sample in the case of Chinese origin accessions (ATFCC), we detected high proportion (15%) of sample heterogeneity. This heterogeneity was not possible to test in USDA, IPK, CGN and JIC samples, as they originated from single plants, while in CzNPC and VIR samples (originated again from bulks of 10 plants per samples) prevailed susceptible alleles. Finally, the susceptible eIF4E^S variant was found in 53.8% to 100% proportion within studied regions, in total 2331 accessions (83%). The lowest occurrence was in 145 studied accessions from India (53.8%), followed by China (63%) and Ethiopia (63%). In contrast, within the 1145 analyzed European origin accessions, including modern pea varieties, it was predominant (97%) (Fig. 2, Table 2). It is clear that there is bias towards European (1145 acc.) compare to other geographical regions, affecting likely allele distribution resulting in S allele over-representation.

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Figure 1. Schematic representation of sequence alignment of the all 19 identified protein eIF4E alleles.

Four principal eIF4E variants identified by intron 3 polymorphism are designated A,B,C and S, while numbers indicate the respective allelic variant. Black bars indicate polymorphism nucleotides within both exons and introns, while red bars indicate polymorphism leading to amino acid exchanges. Horizontal lines indicate insertions/deletions. The heading line indicates nucleotide numbers and exon (solid black boxes)-intron (lines) positions and sizes. Blue arrows indicate primer A combination (Ps-eIF4E-750F and Ps-eIF4E-586gR) and green arrows indicate primer B combination (Ps-eIF4E-750F and Ps-eIF4E-1270R).

https://doi.org/10.1371/journal.pone.0090394.g001

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Figure 2. Geographical distribution of four eIF4E alleles expressed as percentage of total.

Comparison of allele frequencies in 13 geographical regions, as detailed in Table 2.

https://doi.org/10.1371/journal.pone.0090394.g002

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Table 1. Allele designation based on intron 3 length polymorphism, indicating size of obtained fragments with primer combinations A and B, number of repeat motives, intron 3 difference and total length.

https://doi.org/10.1371/journal.pone.0090394.t001

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Table 2. Summary of germplasm screening of 2803 pea accessions, indicating four principal eIF4E intron 3 length variants (A-B-C and S), distribution in total numbers and percentage over 13 geographical regions.

https://doi.org/10.1371/journal.pone.0090394.t002

Isolation of eIF4E sequences from the selected accessions

In order to assess the genetic diversity within the four classes (A, B, C and S) and to test whether intron 3 lengths correspond to DNA and amino acid haplotypes; we sequenced 32, 25, 9 and 7 accessions with respective eIF4E^A-B-C-S variants from selected geographically diverse regions: Turkey, Nepal, Pakistan, Afghanistan, Ethiopia and China (Fig. 2, Table 2, 3, Table S2). The total length of genomic DNA clones of eIF4E^A-B-C-S variants from ATG to stop codons was 2102, 2080, 2208, 1973–2892 bp long, respectively (Table S2), and length polymorphism was largely conferred by 50 and 56 bp insertions/deletions of minisatellite-like repeat sequences located at 102 and 421 bp respectively from the beginning of intron 3 (Fig. 1, Table 1). The extensive variation of eIF4E ^S was due to wild Pisum samples, especially of more distant subspecies (P. abyssinicum) and species (P. fulvum). Beside intron 3 polymorphism, extensive nucleotide variation was found; altogether, there were 156 SNPs identified in 73 sequences (Fig. 1, Table S2) resulting in 34 alleles at whole gene (exones-intrones), 26 at cDNA and 19 alleles at protein levels, respectively. Of these 156 SNPs, 62 were unique, e.g. occurred only in once within the sequenced samples.

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Table 3. List of sequenced 73 accessions assigned to identified 19 protein alleles of four eIF4E ^A-B-C-S intron 3 length variants with indicated country of origin.

https://doi.org/10.1371/journal.pone.0090394.t003

There were four different sequences of susceptible eIF4E^S found within 25 sequenced samples of the cultivated Pisum sativum gene pool and three additional sequences within 7 samples of wild pea species (Table 3, Table S2). Translation of computationally spliced cDNA has resulted in four (eIF4E^{S-1-2-3 and 7}) protein variants within the cultivated genepool and an additional three (eIF4E^S-4-5-6) in P. fulvum and P. abyssinicum (Fig. 3, Table S2). The typically resistant eIF4E^A-1 allele [31], [39], as represented by the PI193835 accession from Ethiopia (genebank sequence number GU289735), differed from the eIF4E^S-1 allele (represented by JI194, genebank sequence number KF053441) by five nucleotide (G185T, C218A, C221A in exon 1, G410A in exon 2 and T687G in exon 3) exchanges, while the eIF4E^A-6 allele (PI269818 accession, genebank sequence number KF053455) differed by an additional two nucleotide (G217C in exon 1, C993T in intron 3) exchanges, one AGC triplet (AGC229-231) deletion, and one single base (C768T) deletion, in addition to 1200 bp intron 3 length similar to susceptible 1201 bp (Table 2, Table S2). Within the 28 sequenced accessions with eIF4E^B intron length variant, we detected seven alleles, both at nucleotide (Fig. 1) and amino acid (Fig. 3) levels. The three eIF4E^B-1-2-3 alleles found in nine sequenced samples differed from the eIF4E^S alleles in 40 SNPs and one nucleotide insertion, resulting in four DNA and three protein variants. The deduced amino acid sequence of the eIF4E^B-1 allele differed from the susceptible eIF4E^S allele at three amino acid (M207I and LD218-219QE) exchanges in exons 4 and 5. Two accessions from Turkey (JI1370, JI1090) differed by an additional three SNPs in exon 1, leading to two amino acid exchanges, V23D and V49A, resulting in two eIF4E^B-2-3 alleles (Table 3). We have detected two eIF4E^C alleles among seven sequenced samples. The eIF4E^C-2 allele of the VIR1859 accession, besides having a 1257bp long intron 3 due to its extra copy of a second 56 bp repeat, differed by two (A56T in exon 1, G1872A in intron 3) nucleotides from the eIF4E^S allele, leading to single amino acid (N19I) exchange at exon 1 (Fig. 3). The remaining six sequences of eIF4E^C were identical to the eIF4E^S allele, except for a 56 bp insertion in intron 3 and a single nucleotide exchange at intron 3 in PI505122 and PI639981, accessions from Albania and Bulgaria (Table 2, S2). We were interested in establishing which, if any, of the eIF4E A, B or C alleles can be found in wild pea. It turned out that all analyzed wild pea accesions displayed the eIF4E^S variant as assessed by intron 3 length. Similarly, sequencing analysis of the seven selected accessions had shown that all P. sativum subsp. elatius, except for P. fulvum JI1007, JI1010 and P. abyssinicum JI1632 accessions with one or two amino acid exchanges, had the typically susceptible eIF4E^S-1 allele (Table 2, 3, S2). All had substantial sequence polymorphism compare to cultivated pea except for P. sativum subsp. elatius JI2630 from the Crimea.

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Figure 3. Summarized amino acid exchanges of identified 19 eIF4E alleles.

Asterix indicates the position of exchanges previously identified as crucial for resistance.

https://doi.org/10.1371/journal.pone.0090394.g003

Haplotype network analysis

Haplotype network analysis was used to reveal relationships between sequences. This showed the clear separation of Pisum sativum subsp. elatius (JI3157, JI1091, JI2630), Pisum fulvum (JI1007, JI1010) and Pisum abyssinicum (JI1632) accessions from cultivated germplasm (Fig. 4). The only exception was Pisum sativum subsp. elatius JI261 (genebank sequence number KF053440) from Turkey, which had a sensitive allele at the amino acid level, while exhibiting a transitory stage towards the eIF4E^B allele at the nucleotide level (Table S2). Moreover, intron 3 was 36 bp longer, with a length of 1187 bp. Interestingly, the JI261 sequence was identical to eIF4E^S until position 956 of intron 3, from which point forward it was similar to the eIF4E^B allele. An important difference was the absence of the exon 4 and 5 exchanges, where it was again identical to the susceptible eIF4E^S allele. The JI261 sequence occurred between the eIF4E^S and eIF4E^B alleles, with 23 mutational steps. A further 24 mutations separated the eIF4E^B alleles of JI1194 (genebank sequence number KF053439) of Afghanistan, ATC7134 (KF053433) of China, and JI1090 (KF053434) and JI1370 (KF053432) of Turkey (Fig. 4). The eIF4E^A alleles identified in Indian accessions (PI347464, PI347494, PI378158) proved to be derived from Ethiopian (PI193835, PIS479), Afghan (PIS468) and Indian (PI356991, ATC1044) accessions, and were identical. A further cluster contained Chinese (ATC6931, ATC6927) and Indian (PI347484, PI347422 and PI347328) origin S alleles, both at nucleotide and amino acid levels (Fig. 4).

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Figure 4. Haplotype network of 34 alleles identified at eIF4E whole gene level, using median-joining network algorithm, implemented in NETWORK.

It is based on total of 156 SNP characters, and excludes 50, 56Table 1. Red colour of symbols indicates accessions tested resistant to P1 PSbMV. Size of symbols is proportional to number of accessions with given haplotype.

https://doi.org/10.1371/journal.pone.0090394.g004

Testing for response to PSbMV infection

The functional status of the 15 alleles of all four detected variants was verified through biological testing of their response to P1 PSbMV infection. All 19 of the pea accessions bearing eIF4E^A-1 tested showed resistance to viral infection i.e. the same phenotype as the non-infected or resistant controls (Table S2). DAS-ELISA testing proved negative, indicating the absence of viral coat protein. Six samples had eIF4E^{A-2-3-4-5-6-7} alleles with single (PI116056, PI193584, JI1788) or multiple (PI269818, PI269774) amino acid exchanges, but none of these changed the expression of resistance in these accessions. The eight tested accessions bearing the eIF4E^B-1 allele split into two phenotypic groups. Three accessions (ATC7134 from China and CGN3311, PIS477 from Pakistan, all eIF4E^B-1) were resistant to the P1 pathotype of PSbMV, as the virus was not able to replicate and spread. The second group of accessions (ATC7140, ATC6928 from China, CGN3319 from Pakistan, all eIF4E^B-1; and JI1090, eIF4E^B-2, from Turkey), showed the typical susceptibility symptoms of infection, vein clearing and chlorotic leaf mosaics, leaf size decrease, internode shortening, and sporadically mild leaf-rolling, 14 days after inoculation by PSbMV. The symptoms were comparable with the response of the sensitive genotypes with the eIF4E^S-1 allele. All tested eIF4E^S-1 (18 accesions), eIF4E^S-2 (1 acc.) and eIF4E^S-3 (3 acc.) displayed sensitive response to P1 PSbMV pathotype (Table S2). We have not been able to test remaining eIF4E^{S-4,5,6 and 7} alleles, due to the shortage of homogenous plant material. Finally, of the three tested accessions with the eIF4E^C-1-2 alleles, only VIR1589 from the Sverdlovsk region of Russia was resistant, while JI1104 and JI1108 from Nepal proved susceptible (Table S2). This corresponded to an amino acid exchange at position N19I in exon 1 between the eIF4E^C-1 and eIF4E^C-2 alleles (Table S2).

eIF4E gene structure and diversity

The pea eIF4E gene is comprised of five exons, interrupted by four intron sequences, with a total length of 2.1 kb [31], which is spliced into a 687 nucleotide long (open reading frame) mRNA [32]. The size polymorphism of intron 3 is caused by 50 and 56 bp insertions of minisatellite-like repeat sequences [31], separated by 259 bp of common intron sequence. This polymorphism led to the initial mapping of the sbm-1 locus [35] and the development of a co-dominant marker for breeding [31]. Previous sequencing of 43 accessions of pea varieties showed four haplotypes of intron 1, eight haplotypes of the intron 2 and three haplotypes of intron 4, while 17 haplotypes were detected in intron 3 sequences ([31] and unpublished). Since all, except PI269774 (Sankia) and PI269818 (Aa134), previously tested susceptible and resistant accessions differed by 50 or 56 bp indels in intron 3 [31], we exploited this length polymorphism to screen a wider pea germplasm population. This led to the identification of novel polymorphisms in four principal variants (eIF4E^S, eIF4E^A-B-C). Another functional eIF4E resistance allele found in the PI269818 and PI269774 accessions was not directly tested in this study, as its polymorphism is more difficult to analyze. It is clear that this type of analysis does not provide a comprehensive dataset, as more subtle, single-nucleotide mutations are missed, as documented by sequenced samples, whereas in 73 sequences, 19 eIF4E alleles were found (Fig. 1, Table 3, S1).

The mutant eIF4E^A-1 allele of pea gene eIF4E differs from its eIF4E^S-1 counterpart in five non-conservative amino acid exchanges in and around the cap-binding pocket of pea eIF4E, which impacts infection by PSbMV [32]. Previously reported mutations in pea eIF4E were localized on the β1 (W62L), β1-β2 (AA73-74DD), β3-β4 (G107R) and β5 (N169K) loops [42]. Positions of the newly identified amino acid (V23D, V49A and LD218-219QE) changes in eIF4E^B-1-2-3 alleles do not correspond with the known mutations leading to resistance and are not localized on the variable β loops regions, with the exception of the M207I change located on the α3-β7 loop. The only resistance determinant mapped to the β7 loop is for BaYMV infection in barley [54]. In the Capsicum species, several eIF4E haplotypes with amino acid changes were reported, some of which included amino acid changes in positions 218 and 219 similar to those in the pea eIF4E^B allele. Systematic testing of individual mutations by in planta assay showed that only a specific combination of mutations leads to resistance [42]. This hypothesis confirms previously reported amino acid changes in pea eIF4E in PI269818 and PI269774 accessions [31] with eIF4E^A6-7 alleles, which have amino acid changes in the cap-binding pocket [31] and lack one (S78) amino acid (Fig. 3, Table 3 and Table S2, Supporting information). Amino acid changes in the loop near the cap-recognition pocket were shown to be directly associated with resistance to potyviruses in pepper, lettuce, and pea [35], [55]. Moreover, not all eIF4E proteins encoded by the resistance alleles are defective in their ability to bind the m7-GTP cap, suggesting that disrupted cap binding is not always required for potyvirus resistance [56]. Thus mutations conferring sbm-1 resistance to PSbMV act combinatorially [42]. The interactions between particular eIF4E and particular viral VPgs are highly specific for host-virus interactions. Higher plants are unique in that they encode two distinct isoforms of eIF4F that have both overlapping and isoform-specific roles, eIF4F protein complex, which contains eIF4E and eIF4G, and eIF(iso)4F protein complex, which contains eIF(iso)4E and eIF(iso)4G [25]. Although these two complexes seem to be equivalent for the in vitro translation, they differ in their in vivo expression patterns and specificity for cellular mRNAs and likely also viral RNA. Potyviruses could selectively use either eIF(iso)4E or eIF4E to infect plants, while some are able to use both [39]. In pepper, the combination of eIF4E (pvr2 locus on chromosome 4) and eIF(iso)4E (pvr6 locus on chromosome 3) resistance alleles showed a complementary effect [45]. Also in pea, the eIF(iso)4E homologous gene exists, mapped to LG II ([35] and close to the sbm-2 locus (not shown). However, its direct involvement in potyvirus resistance still needs to be demonstrated. Thus we cannot exclude the possibility that interaction between eIF4E and eIF(iso)4E proteins may also play a role in pea. This might partly explain the discrepancies in virological testing of eIF4E^B accessions. Finally, the result of the interaction, the resistance or tolerance, depends not only on the genetic background but also on the virus pathotype [43], [57]. It would be interesting to test region-specific potyvirus isolates [58] on the alleles identified in this study. The current phylogeny of potyviruses indicates that the progenitor probably infected plants growing in southwestern Eurasia and northern Africa, which evolved from a rymovirus by acquiring the ability to be transmitted by aphids [48]. It is notable that we did not find the resistant eIF4E^A variant in any of the 146 tested wild pea samples, nor have we been able to detect any intermediate alleles between the susceptible eIF4E^S-1 and resistant eIF4E^A-1, except in JI261 accessions already separated by 23 mutations between eIF4E^S-1 and eIF4E^B-1. Such an intermediate allele was found for Pm3 powdery mildew resistance in wheat from the Himalayan Range, supporting the hypothesis that a recent evolution of these alleles took place in the hexaploid wheat gene pool [8]. This is a departure from the situation with other diseases for which resistance can often be found within the wild genepool, in contrast to the domestic gene pool. i.e. pathogens overcame resistance in the domestic gene pool. Haplotype analysis indicated the separate evolution of eIF4E^A alleles, with Ethiopian and Indian accessions being the most distantly related, while Chinese and some Afghan and Nepalese accessions were closer to eIF4E^S-1 (Fig. 4). Thus the evolution of the eIF4E gene is demonstrated in the available pea genepool.

Geographical distribution of eIF4E variants versus PSbMV diversity

Pea genotypes resistant to PSbMV were identified by Hagedorn and Gritton [59] in two Ethiopian lines (PI193586 and PI193835). Later, Hampton [60] found several resistant accessions from India. Since they are all identical in sequence [31], it raises the question of their origin. Hampton [60] proposed that India may have been the center of origin for the virus, and consequently also the center of PSbMV-immune germplasm. Despite this resistant germplasm, pea has not been systematically bred to include PSbMV resistance until recently; as a consequence, the vast majority of pea varieties are susceptible to this virus [31], especially when contrasted with landraces where the possibility of both natural and human selection for resistance exists. A relationship between pea host and PSbMV pathogen diversity in northern Pakistan and Afghanistan has been proposed [60], [61]. More recently, phylogenetic analysis of PSbMV isolates from Australia and China grouped them on separate clades [55]. These results suggest the operation of co-evolutionary forces, which could be tested using the eIF4E alleles identified in this study. Interestingly, similar results were found in a study of barley eIF4E haplotype diversity, which was also found to be considerably higher in Central and East Asia, both regions with a long history of the bymovirus disease [3]. It is intriguing to see that in different crops, the resistance diversity center is outside the original place of domestication, especially in Central and Eastern Asia. It might be hypothesized that only some environments may be conducive for pathogen development in crops, hence co-evolution may be geographically limited. This agrees with results showing that the eIF4E gene is under positive selection pressure [3], [44]. There is strong selection for the eIF4E^B allele in China and less elsewhere (Fig. 2, Table 2), but no A or C alleles in China, possibly consistent with a genetic bottleneck in the later migration of pea to China, compared with central/western Asia [18]. Moreover, assignment of Chinese accessions to provinces showed preferential occurrence of B alleles in the autumn/winter-sown provinces of Henan, Anhui, Hubei and Sichuan (Table S1) that experience high rainfall, long growing seasons and cold, frost-prone vegetative phases. Conversely, S alleles predominated in spring-sown types grown in northern provinces with low rainfall and high, variable temperatures that result in severe drought [62]. Analysis of pea germplasm diversity, using retrotransposon-based insertion polymorphism [13], [14], [63] showed that although there is substantial genetic diversity present, it is only partially geographically structured. Especially in case of the eIF4E^B alleles, there is substantial clustering of Indian and Ethiopian accessions, indicating a close relationship between materials from these areas (Fig. 5). The number of mutations makes independent origin unlikely, unless stringent positive selection is operating, as detected by Cavatorta [44]. We have also found evidence of positive selection in a set of 27 tested pea cDNA haplotypes (not shown). As proposed for barley eIF4E, the explanation for the unusually high overall degree of eIF4E sequence variation and haplotype diversity may be that these are important for adaptation to different local habitats [3]. The second, more plausible scenario is introgression and maintenance in populations by selective advantage. Based on the number of accesions with detected eIF4E^A and eIF4E^B alleles in the northern Indian subcontinent versus Ethiopia/eastern Africa, we speculate that these more likely originated in the Africa and were then brought by oversea trade to Afghanistan-Pakistan-Nepal-India region. The Silk Route, Amber Road and trans-Saharan trade routes were all instrumental in establishing links between Africa, India and beyond. This scenario, while not proven, is supported by a haplotype analysis network, where Indian eIF4E^A alleles were shown to be derived from Ethiopian accessions (Fig. 4). There is bias towards European origin accesions, comprising 40.8% of samples, followed by Ethiopia and East Africa (12.4%) and Russia (10.5%) which consequently affects proportion (57%) of S alleles (Table 2). However, these samples were included to provide more complete view on eIF4E^A diversity, including modern pea varieties. However this affects only percentage in total dataset and not within studied regions (Fig. 2, Table 2).

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Figure 5. Visualization of genetic relationship of four identified eIF4E variants on the background of 14 BAPS identified clusters of 3,029 JIC accessions genotyped by 45 retrotransposon-insertion loci [13], [63].

Notably, clusters 3 and 4 contain 145 acc. of wild P. fulvum, P. s. subsp. elatius and P. abyssinicum; cluster 7 contains 95 acc. from Afghanistan; cluster 8 has 225 acc. from Ethiopia; and cluster 9 contains 247 acc. from India and Ethiopia, while remaining clusters are more diverse (A). 18 accessions with the eIF4E^A (B), 241 accessions with the eIF4E^B (C), 81 accessions with the eIF4E^C variant (D).

https://doi.org/10.1371/journal.pone.0090394.g005

Various approaches have been applied to identify variants of the eIF4E gene in several crops. These showed different levels of diversity [3], [43], [46], [47]. In the most comprehensive study, 1090 barley samples from 84 countries screened using the high-resolution melting PCR method of cDNA, led to the identification of 47 eIF4E haplotypes [3]. In our study, we have identified 34 alleles on exon-intron level with 156 single-nucleotide polymorphic sites in total of 73 sequences. This suggests that substantial diversity within the eIF4E gene can be expected within available pea germplasm, which might be fully revealed only by sequencing. It is notable that none of the 146 tested wild pea samples had eIF4E^A or eIF4E^B alleles, but at the amino acid level, all corresponded to the susceptible eIF4E^S-1 allele, despite substantial polymorphism both in introns and exons, indicating selection for functionality. The discovery of the intermediate allele in wild P. sativum subsp. elatius JI261 from Turkey is similar to data obtained in Arabidopsis thaliana [25], which suggests a distinct mode of evolution of resistance in wild species as opposed to crop species and supports the scenario in which potyviruses spread at the advent of agriculture, which brought plants into dense monocultures [48]. Very little is known about the selective forces that drive viral evolution in natural ecosystems, which contrasts with the more detailed population genetics studies in crop plants that have revealed the importance of mutation rates, recombination, genetic drift and migration in virus evolution [64]. Although we can not fully excluded the possibility, that JI261 allele could have resulted from domestic to wild introgression and not independent mutations, the analysis of 45 RBIP loci (Fig. 5) clustered it with typical P. s. subsp. elatius accessions [63]. The existence of resistance alleles only in the domesticated pea genepool leads us to speculate that the mutation originated during early cultivation. The series of alleles identified in this study provide an excellent basis for testing various potyviruses and pathotypes in which to study the co-evolution of potyviruses and their pea host.

Plant material

The 2803 pea accessions used in this study were drawn from the following collections: the Czech National Pea Collection (CzNPC, 1252 accessions); the USDA core collection (384); 119 accessions of Chinese origin from the Australian Temperate Field Crops Collection [(ATFCC) now reconstituted as Australian Grains Genebank, (AGG)], the Vavilov Institute (VIR) Russian Federation (69); the Centre for Genetic Resources (CGN), The Netherlands (14); Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany (35); and John Innes Centre (JIC), UK (836). The permission from the relevant institutions was obtained to access the collections, and the respective pea germplasm accessions were donated for this study under Standard Material Transfer Agreement of germplasm resources. Although we specifically targeted local landraces rather than globally grown, modern varieties, the analyses of 1252 accessions from the Czech National Pea collection represent the diversity present in the genepool of cultivated pea in the 19^th to 21^st centuries [16], [49]. This collection is composed of 972 commercial varieties, 226 breeding lines and 54 landraces, which originate largely from Europe (925), the former Soviet Union (177), and the USA and Canada (77). In contrast, the John Innes Centre pea collection has, in addition to varieties (1071) and breeding lines (61), a large proportion of landraces (600), mutant stocks (585) and wild peas (445). Geographically, this collection is dominated by accessions of Ethiopian (388), Mediterranean (199), Indian (53) and Chinese (37) origin. The Australian ATFCC and USDA pea collections are partially complementary. The former includes the diversity core set of Chinese origin [18], of which 119 were studied here, while the latter has over 6000 accessions, of which a core set of 384 [19] was analyzed in this study. Finally, 69 accessions selected from the VIR collection originated in the Caucasus and Central Asia (Turkmenistan, Kazakhstan, Georgia, and Armenia) as well as in North Africa (Morocco and Algeria). It is worth mentioning that although Ethiopia is known as the origin and occurrence for P. abyssinicum, the accessions used in this study were mainly P. sativum subsp. sativum. This set was complemented with wild P. fulvum (9), P. sativum subsp. elatius (86), P. sativum subsp. sativum (19) (formerly P. humile/P.syriacum) (see Smýkal et al. [13] for taxonomical classification) and P. abyssinicum (32) (Table S1). These represent both primary and secondary centers of pea diversity as well as primary and secondary gene pools.

DNA isolation and PCR analysis

Young leaves were harvested from ten (in the case of the Czech, ATFCC, CGN, IPK and VIR collections) or single (in the case of the JIC and USDA collections) randomly chosen plants per accession and stored at −80°C. Genomic DNA was isolated and PCR performed using standard protocols [31]. Products were resolved on 1.5% TBE agarose gel and visualized using ethidium bromide staining under UV-light.

eIF4E variants amplification

Intron 3 length polymorphism was used as screening criteria, as developed by Smýkal et al. [31]. In short, differences in intron 3 length were assayed using PCR with the following pairs of primers: Ps-eIF4E-750F (5′-GGACTAAGAATGCTTCAAATGAAGCTGC-3′) and Ps-eIF4E-586gR (5′-GAATCATTTAAGAAGCTCGTGAAGTG-3′) primers (combination A, nested within the combination of B) that amplified 243 bp in the resistant and 293 bp in the susceptible accessions [31]; and (combination B) Ps-eIF4E-750F and Ps-eIF4E-1270R (5′-ATTCTCGATCACACTAGCCCCCTCC-3′) [35] primers that amplified 536 bp versus 586 bp fragment. The combination of these two assays resulted in the detection of respective eIF4E^A-B-C-S variants (Fig. 1, Table 1). PCR amplification of 1000 bp product from start codon to intron 3 was carried out using primers Ps-eIF4E-1F start codon (5′-ATGGTTGTAGAAGACACCCCCAAATC-3′) and Ps-eIF4E-586gR. Primers Ps-eIF4E-794F (5′-GCTAGATGGTTGTTATGATGTTTATCAG-3′) and Ps-eIF4E-2188R stop codon (5′-TTGCTAGTTTGCTACCATGTAAGAACG-3′) were used to amplify a 1500 bp PCR product spanning the rest of eIF4E gene from intron 3 to exon 5. The PCR products were purified according to Werle et al. [50] protocol and sequenced using a BigDye Terminator kit (Applied Biosystems, UK) by Macrogene (Amsterdam, The Netherlands).

Bioinformatics

Primer design and restriction analyses were performed using FastPCR software version 5.1.83 (PrimerDigital Ltd., Finland). The DNA sequences were viewed and edited using Sequence Scanner version 2.0 (Applied Biosystems, UK). CLUSTALW alignment was performed using BioEdit version 7.09.0 [51]. The haplotype alignments were performed using a median-joining network algorithm, implemented in NETWORK 4.5.1.6 [52]. To reveal the genetic relationship of samples, we used previously made Bayesian clustering analysis of the genetic diversity of 3,029 JIC accessions genotyped by 45 retrotransposon-insertion loci [13]. Identified clusters were used as framework to visualize individual eIF4E alleles within the set of 836 JIC accessions analyzed in this study. Single nucleotide polymorphism mapping to reference sensitive allele was done using Geneious 6.1.6 analysis software (Biomatters, USA).

Virological testing

Plants were grown in a substrate Klassman no. 4 (Klasmann-Deilmann GmbH, Germany) in a growth chamber (Microclima 1000, Snijders Scientific, Holland) under a 16/8-h and 22/18°C day/night cycle. Evaluation of resistance/susceptibility to the PSbMV pathotype P-1 was conducted through mechanical inoculation using isolate PSB117CZ [31], [53]. All together, 50 accessions were tested. Ten plants per accession were tested in same block, under the same conditions. Sensitive pea cultivars, Merkur and Raman, were used as a sensitive and B99 as a resistant controls [31]. Symptoms were observed at one-week intervals, and systemic infection was confirmed three weeks after infection with DAS-ELISA (Loewe Biochemica, Germany).