The reemergence of dengue as an important public health problem reflects the difficulties in sustaining vertically organized, effective, control programs and the need for community-based strategies for Aedes aegypti control that result in behavioral change. We aimed to disentangle the relationships between underlying determinants of dengue related practices. We conducted a cross-sectional study in 780 households in La Lisa, Havana, Cuba. A questionnaire and an observation guide were administrated to collect information on variables related to economic status, knowledge on dengue, risk perception and practices associated with Aedes aegypti breading sites. To test a conceptual model that hypothesized direct relationships among all these constructs, we first used Exploratory Factor Analysis with Principal Component Analysis to establish the relationship between observed variables and the underlying latent variables. Subsequently, we tested whether the observed data supported the conceptual model through Confirmatory Factor Analysis. Exploratory Factor Analysis indicated that the items measured could be reduced into five factors with an eigenvalue >1.0: Knowledge on dengue, Intradomiciliar risk practices, Peridomiciliar risk practices, Risk perception and Economic status. The proportion of the total variance in the data explained by these five factors was 74.3%. The Confirmatory Factor Analysis model differed from our hypothesized conceptual model. Only Knowledge on dengue had a significant, direct, positive, effect on Practices. There was also a direct association of Economic status with Knowledge on dengue, but not with Risk perception and Practices. Clarifying the relationship between direct and indirect determinants of dengue related practices contributes to a better understanding of the potential effect of Information Education and Communication on practices and on the reduction of Aedes aegypti breeding sites and provides inputs for designing a community based strategy for dengue control.
Citation: Castro M, Sánchez L, Pérez D, Sebrango C, Shkedy Z, Van der Stuyft P (2013) The Relationship between Economic Status, Knowledge on Dengue, Risk Perceptions and Practices. PLoS ONE 8(12): e81875. https://doi.org/10.1371/journal.pone.0081875
Editor: Julian W. Tang, Alberta Provincial Laboratory for Public Health/University of Alberta, Canada
Received: June 28, 2013; Accepted: October 19, 2013; Published: December 12, 2013
Copyright: © 2013 Castro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was funded by the Cuban Ministry of Health and through a framework agreement between the Institute of Tropical Medicine ‘Pedro Kouri’ (Havana City, Cuba), the Institute of Tropical Medicine (Antwerp, Belgium) and the Belgium Directorate-General for Development Cooperation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Dengue infections currently occur in more than 100 countries in the Asia-Pacific region, the Americas, the Middle East, and Africa . The reemergence of dengue as an important public health problem reflects the difficulties in maintaining vertically organized, effective, control programs and the need to develop sustainable, integrated approaches to Aedes aegypti control , . Community participation and changes in human behaviors are essential components , , , since programs that include information, education and communication and strengthen active participation achieve better results –.
Dengue KAP surveys have frequently been used to describe knowledge, attitudes and practices of the community towards prevention –. They can identify knowledge gaps, cultural beliefs or behavioral patterns that form barriers for action, and assist in the design of promotion activities –. They have also been used as a tool to assess the effectiveness of health education programs and community based strategies –, –. However, a review on dengue prevention indicates that there is little evidence on how information from KAP surveys was used to plan for subsequent participatory strategies . Furthermore, while education campaigns have increased people’s knowledge on dengue, it remains unclear to what extent this led to behavioral change and actually reduced mosquito populations .
KAP studies indicate that the relationships between determinants of human behaviour with bearing on the control of Ae. aegypti are complex . Some studies used regression models to examinate the direct relationships , –, , , but the challenge is to characterize the behavioral system and to understand the direct and indirect effects of the determinants. Latent variable structural equation modeling provides a tool to address this challenge and allows for the quantification and testing of hypothesized relationships among latent and observed variables .
The present study was carried out as part of the formative research prior to developing a community-based strategy for dengue prevention , . Our aim was to disentangle the relationships between Economic status, Knowledge on dengue, Risk perception and dengue related Practices.
Materials and Methods
Study Area and Design
The study was conducted in La Lisa municipality, Havana, Cuba. The municipality has about 30 000 inhabitants, and an area of 37.5 km2. The population is concentrated in residential neighborhoods with urban characteristics; at the periphery the territory is semi-urban to rural. Three amongst the seven “Consejos Populares” (CP: an intermediate governance structure between the municipal level and the “circunscripción”, the lowest level of local government that covers about 1 000 inhabitants), Versalles-Coronela, Alturas de La Lisa and Balcon Arimao are high-risk areas for dengue transmission, with House Index (number of houses with at least one container with Ae. aegypti larvae/100 houses examined) higher than 2% . We conducted a descriptive cross-sectional survey in these CPs in November 2004. 32 circumscriptions were randomly selected and the heads (or, if absent, another adult resident) of 780 randomly selected households were interviewed.
Underlying Conceptual Model
Based on previous research , , , , , measure of Economic status, Knowledge on dengue, Risk perception and Risk practices for dengue were collected. The underlying hypothesis (Figure 1) were that better economic status is directly associated with decreased risk practices for dengue, with higher knowledge on dengue and with increased risk perception. Secondly, that knowledge is associated with a higher risk perception and better practices. Finally, that higher risk perception is associated with decreased risk practices for dengue.
Trained local health volunteers used two instruments for the study: a questionnaire created and validated previously  and an observation guide elaborated to assess risk behavior associated with Ae. aegypti breading sites.
The questionnaire covered socio-demographic information (age, education); knowledge on dengue symptoms (measured as the number of correct symptoms that respondents mentioned amongst fever, headache, nausea/vomiting, muscular pain, rash or bleeding); knowledge of preventive measures (the number of measures mentioned amongst adding temephos to water storage containers, covering other useful containers, eliminating useless containers, spraying insecticide); and risk perception (the risk to contract dengue fever and whether dengue infection could be fatal, both scaled as none(1), moderate(2), or high(3)). In addition, we ascertained the respondents number of information sources on dengue (e.g. television, radio, newspaper, local leaders, doctors, nurses, local meetings). Finally, a scale was developed for owning items related to economic status. An air-conditioning system, video recorder, telephone, car or microwave were given 3 points each; a refrigerator, fan, washing machine or mixer 2 points; and a radio, sewing machine, pressure cooker or tape recorder each 1 point. All points were summed to obtain a household asset score.
The practices related to dengue that we observed were the number of water storage containers that were badly covered or in bad condition, the number of other small containers outside the house and the number of useless containers in the backyard.
Firstly, we carried out a descriptive analysis of the KAP survey. After that, an Exploratory Factor Analysis (EFA) was conducted to determine the best factor structure to represent the relationships between economic status, knowledge on dengue, risk perception and dengue related practices. A Principal Component Analysis (PCA) was applied using orthogonal rotation (Varimax). Those factors with an eigenvalue larger than 1 were retained. We tested whether the observed data supported the underlying conceptual model with a Confirmatory Factor Analysis (CFA) . To perform the CFA we followed six basic steps according Hatcher : 1. Define the factor model (the underlying conceptual model); 2. Collect measurements; 3. Obtain the correlation matrix; 4. Fit the model to the data (Maximum likelihood estimation); 5. Evaluate model adequacy (chi-square test and goodness-of-fit test); and 6. Compare with other models (comparing a full model with a reduced model by examining the difference between their X2 statistics).
All statistics were performed with SPSS 15.0 for Windows except the CFA for which SAS PROC CALIS was used. We drew the path diagram with Graphviz 2.26.3. All p-values are two-tailed and considered statistically significant at the 5% level.
The study was approved by the Research and Ethics Committee of the Institute Pedro Kouri, the Infectious Diseases Research Committee of the Cuban Ministry of Health and the municipal health authorities. The participants were provided with a verbal and written explanation of the objectives and procedures of the study. Written informed consent was obtained from all respondents prior to the interview. The ethic committee waived the need for informed consent from the next of kin, caretakers, or guardians on behalf of respondents aged 16 or 17. The topic explored was not considered as a personal or sensitive issue for such ages. During the investigation process, no information that could distinguish individual respondents was collected.
Table 1 summarizes the characteristic of the 779/780 respondents for whom complete information was available. Approximately 50% of the respondents were between 30–59 years old and 60% had completed secondary education. They mentioned, on average, correctly 3 dengue symptoms and 4 preventive measures. 39% of the respondents thought they were not at risk to contract dengue, but more than 87% referred that dengue could be highly fatal. The mean number of badly covered water storage containers and of useless containers in the backyard was one and 5 per household, respectively. The mean household asset score was 15.
Exploratory and Confirmatory Factor Analysis
EFA (Table 2) showed that the 10 items measured could be reduced into five factors with an eigenvalue >1.0: Knowledge on dengue, Intradomiciliar risk practices, Peridomiciliar risk practices, Risk perception and Economic status. The proportion of the total variance in the data explained by these five factors was 74.34%.
Figure 2 present the results of the CFA and tests the relationships hypothesized in the underlying conceptual model. The CFA model with the five factors from EFA fitted the data well (χ2 = 30.28, df = 32; p = 0.553). The Goodness-of-fit was also revealed by high values of the adjusted goodness-of-fit index (0.98).
La Lisa municipality, Havana City, Cuba.
There were statistically significant correlations between the five factors (latent variables) and all the corresponding observed variables. We confirmed part of the hypothesized direct relationships between factors. Knowledge on dengue had high negative correlations with Intradomiciliar and Peridomiciliar risk practices and a positive correlation one with Risk perception. However, Economic status had a direct significant relationship with Knowledge on dengue only. Although Risk perception in itself was positively related with Peridomiciliary risk practices, it was not related with Intradomiciliry risk.
The CFA model differs from our hypothesized conceptual model, with only Knowledge on dengue having a significant and beneficial direct effect on Practices. However, our results have been obtained with a cross-sectional design and the observed relationships should be interpreted with caution. Still, the study included a large random sample of respondents, and the detailed measurements should strengthen the evidence obtained.
Previous KAP studies on dengue provide conflicting results. Some showed that gaining knowledge does not lead to changing practices , , . Others provided evidence of a relationship between knowledge of preventive measures and best household practices –, , , , . Notwithstanding, studies in Thailand , Trinidad and Tobago  and Brazil  found little or no correlation between knowledge on dengue and Ae. aegypti infestation levels. Furthermore, studies presenting evidence on a relationships between knowledge and practices and Aedes populations are scarce , , . It should be noted that there are methodological differences among the cited studies. The main variables were operationalized in various ways. Some authors determined knowledge on dengue as knowledge of symptoms and other disease related aspects such severity or as transmission modes , , , , , , . Other authors included knowledge on the vector breeding sites and a wide range of prevention measures (i.e. covering waters containers, using repellents, and bed nets) , , . Few others used a score to measure overall dengue knowledge , . Something similar is observed in the way actual dengue preventive practices, or their effects, were defined and assessed. Some studies used practices reported by respondents or observed at the time of the study , , , , whereas others used information on Ae. aegypti larvae, pupal or adult surveys , , , , . On the other hand, the methods used to explore the relation between knowledge and practices ranged from calculating association measures to fitting regression models. This should also influence the obtained results.
Perhaps more importantly, associations observed in a particular context may possibly not be extrapolated to other settings. The results of this study suggest a direct association of economic status with dengue knowledge. People with higher economic status could have better access to information through multiple channels (television, radio, newspapers). Combined with the effect of generalized education, this could assure a better understanding and comprehension of information when accessed. On the other hand, economic status was not associated with risk perception and practices.
The way economic status was assessed in this study could be considered a study limitation. Durable material goods owned household level might not reflect the current income of the households. Furthermore, in the Cuban context determinants of health related the economic status, such as access to health services, to social security, to education and to basic food are free or heavily subsidized. Besides, it is difficult to obtain reliable information on real income. However, it is very expensive to acquire durable material goods with an average Cuban monthly salary and supplementary sources of income are needed to do so. Therefore these goods constitute proxy indicators of economic status.
Risk perception was not significantly associated with dengue related practices. This could have several possible explanations. Populatiońs personal experience with the disease could be limited since dengue is not endemic in Cuba and outbreaks occur only from time to time and are rapidly controlled . Besides, communication activities on dengue decrease during inter-epidemic periods. On the other hand, there are contextual cultural factors that influence the practices. In general, there is a technocratic and paternalistic approach to health care. As a result the population does not recognize the importance of its actions in controlling Ae. aegypti ,  and has shifted the responsibility to the health sector and other governmental institutions .
With regard to increased knowledge and practices for dengue prevention, studies conducted in Santiago de Cuba and Havana , ,  showed increased knowledge and adoption of best practices for the reduction of Ae. aegypti breeding sites after health education activities. Our study confirms this relationship and suggests that emphasis should be put on concrete ways to prevent dengue.
More generally, we showed that the use of EFA and CFA can clarify the relationship between direct and indirect determinants of dengue related practices and contribute to a better understanding of the potential effect of Information, Education and Communication strategies on the reduction of Ae. aegypti breeding sites. In doing so, our study provided inputs for designing a community based strategy for vector control ,  and offers a tool for assessing perceptions and practices related to dengue at household level.
Conceived and designed the experiments: LS DP PVDS. Performed the experiments: MC LS DP. Analyzed the data: MC LS CS ZS PVDS. Contributed reagents/materials/analysis tools: LS CS ZS. Wrote the paper: MC LS DP CS PVDS.
- 1. OMS (2012) Dengue and Dengue haemorrhagic fever: fact sheet 117. Available: http://www.who.int/mediacentre/factsheets/fs117/en/index.html. Accessed 2012 Apr 28.
- 2. Gluber DJ (2002) Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol 10: 100–103.
- 3. Elder J, Lloyd LS (2006) Achieving Behaviour Change for Dengue Control: Methods, Scaling-Up, and Sustainability. In: Report of the scientific Working Group on Dengue. WHO, Geneva: 140–149. Available: http://www.who.int/tdr/publications/publications/swg_dengue_2.htm Accessed 2008 Jan 12.
- 4. Sanchez L, Perez D, Pérez T, Sosa T, Cruz G, et al. (2005) Intersectoral coordination in Aedes aegypti control. A pilot project in Havana City, Cuba. Trop Med Int Health 10: 1–10.
- 5. Sanchez L, Perez D, Cruz G, Castro M, Kouri G, et al. (2009) Intersectoral coordination, community empowerment and dengue prevention: six years of controlled interventions in Playa Municipality, Havana, Cuba. Trop Med Int Health 14(11): 1356–64.
- 6. Toledo ME, Vanlerberghe V, Baly A, Ceballos E, Valdes L, et al. (2007) Towards active community participation in dengue vector control: results from action research in Santiago de Cuba, Cuba. Trans R Soc Trop Med Hyg 101: 56–63.
- 7. Toledo Romani ME, Vanlerberghe V, Perez D, Lefevre P, Ceballos E, et al. (2007) Achieving sustainability of community-based dengue control in Santiago de Cuba. Soc Sci Med 64: 976–88.
- 8. Parks WJ, Lloyd LS (2004) Planificación de la movilización y comunicación social para la prevención del Dengue: guía paso a paso. Washington, DC: OPS.
- 9. Donalisio MR, Alves MJ, Visockas A (2001) Inquérito sobre conhecimentos e atitudes da população sobre a transmissão do dengue-região de Campinas São Paulo, Brasil-1998. Rev Soc Bras Med Trop 34: 197–201.
- 10. Acharya A, Goswami K, Srinath S, Goswami A (2005) Awareness about dengue syndrome and related preventive practices amongst residents of an urban resettlement colony of South Delhi. J Vector Borne Dis 42: 122–127.
- 11. Koenraadt CJ, Tuiten W, Sithiprasasna R, Kijchalao U, Jones JW, et al. (2006) Dengue knowledge and practices and their impact on Aedes aegypti populations in Kamphaeng Phet, Thailand. Am J Trop Med Hyg 74(4): 692–700.
- 12. Itrat A, Khan A, Javaid S, Kamal M, Khan H, et al. (2008) Knowledge, Awareness and Practices regarding dengue fever among the adult population of Dengue Hit Cosmopolitan. PLoS ONE 3(7): e2620 Doi:https://doi.org/10.1371/journal.pone.0002620.
- 13. Shuaib F, Todd D, Campbell-Stennett D, Ehiri J, Jolly PE (2010) Knowledge, attitudes and practices regarding dengue infection in Westmoreland, Jamaica. W Indian Med J 59(2): 139–146.
- 14. Naeem-Ullah U, Akram W (2009) Dengue knowledge, attitudes and practices in Multan, Pakistan: An urban area at the verge of dengue infestation. Public Health 123: 452–453.
- 15. Parks WJ, Lloyd LS, Nathan MB, Hosein E, Odugleh A, et al.. (2004) International experiences in Social Mobilization and Communication for Dengue Prevention and Control Dengue Bull: 1–7.
- 16. O’ Donell CR, Yamauchi LA (2005) Culture and context in human behaviour change: Theory, research and application. New York: Peter Lang Publishing.
- 17. Heintze C, Garrido MV, Kroeger A (2007) What do community- based dengue control programmes achieve? A systematic review of published evaluations. Trans R Soc Trop Med Hyg 101: 317–325.
- 18. Swaddiwudhipong W, Chaovakiratipong C, Nguntra P, Koonchote S, Khumklam P, et al. (1992) Effect of health education on community participation in control of Dengue hemorrhagic fever in an urban area of Thailand. Southeast Asian J Trop Med Public Health 23: 200–206.
- 19. Rosenbaum J, Nathan MB, Ragoonanansingh R (1995) Community participation in dengue prevention and control: a survey of knowledge, attitudes, and practice in Trinidad and Tobago. Am J Trop Med Hyg 53: 111–117.
- 20. Lloyd LS, Winch P, Ortega-Canto J, Kendall C (1992) Results of a community-based Aedes aegypti control program in Merida, Yucatan, Mexico. Am J Trop Med Hyg 46: 635–42.
- 21. Leontsini E, Gil E, Kendall C, Clark GG (1993) Effect of a community- based Aedes aegypti control programme on mosquito larval production sites in El Progreso, Honduras. Trans R Soc of Trop Med Hyg 87: 267–271.
- 22. Chiaravalloti F, de Moraes MS, Fernandes MA (1998) Result of activities encouraging community participation in dengue controlin an outlying neighborhood of São Jose do Rio Preto, São Paulo, and the relationship between the population’s knowledge and habits. Cad Saude Publica 14: 101–109.
- 23. Winch PJ, Leontsini E, Rigau-Perez JG, Ruiz-Perez M, Clark GG, et al. (2002) Community-based dengue prevention programs in Puerto Rico: impact on knowledge, behavior, and residential mosquito infestation. Am J Trop Med Hyg 67: 363–370.
- 24. Nam V, Kay B, Yen N, Ryan P, Bektas A (2005) Community mobilization, behaviour change and biological control in the prevention and control of dengue fever in Viet Nam. Dengue Bull 28: 57–61.
- 25. Kay BH, Nam VS, Tien TV, Yen NT, Phong TV, et al. (2002) Control of Aedes vectors of dengue in three provinces of Vietnam by use of Mesocyclops (Copepoda) and community-based methods validated by entomologic, clinical, and serological surveillance. Am J Trop Med Hyg. 66(1): 40–8.
- 26. Van Benthem BHB, Khantikul N, Panart K, Kessels PJ, Somboon P, et al. (2002) Knowledge and use of prevention measures related to dengue in nothern Thailand. Trop Med Int Health 7: 993–1000.
- 27. MacCallum RC, Austin JT (2000) Applications of structural equation modeling in psychological research. Annu Rev Psychol 51: 201–226.
- 28. Castro M, Pérez D, Pérez K, Polo V, López M, et al. (2008) Contextualization of a comprehensive community-based strategy for dengue prevention [in Spanish]. Rev Cubana Med Trop 60: 83–91.
- 29. Castro M, Sánchez L, Pérez D, Carbonell N, Vanlerberghe V, et al. (2012) A community empowerment strategy embedded in a routine dengue vector control programme: a cluster randomised controlled trial. Trans R Soc of Trop Med Hyg 106: 315–321.
- 30. Bollen KA (1989) Structural equations with latent variable, New York: John Wiley & Sons.
- 31. Hatcher L (1994) A step-by-step approach to using the SAS system for factor analysis and structural equation modeling. Cary, NC: SAS Institute Press.
- 32. Degallier N, Vilarinhos PT, de Carvalho MS, Knox MS, Caetano Jr J (2000) People’s knowledge and practice about dengue, its vectors, and control means in Brasilia (DF), Brazil: its relevance with entomological factors. Journal of the American Mosquito Control Association 16: 114–123.
- 33. Ballenger-Browning K, Elder JP (2009) Multi-modal Aedes aegypti mosquito reduction interventions and dengue fever prevention. Trop Med Int Health 14(12): 1542–51.
- 34. Guzman MG, Kouri G (2009) Dengue in Cuba: research strategy to support dengue control. Lancet 14 374(9702): 1660–1661.
- 35. De la Cruz AM, Figueres D, Chacón L, Gómez M, Díaz M, et al. (1999) Conocimientos, opiniones y prácticas sobre Aedes aegypti.. Rev Cubana Med Trop 51: 135–137.
- 36. Toledo-Romaní ME, Baly-Gil A, Ceballos-Ursula E, Boelaert M, Van der Stuyft P (2006) Participación comunitaria en la prevención del dengue: un abordaje desde la perspectiva de los diferentes actores sociales. Salud Publica Mex 48: 39–44.
- 37. Acosta OS, Chalgub AM, Baydes RO, Abraham EA (1999) Modificación de los conocimientos, actitudes y prácticas de la población sobre la prevención de los mosquitos. Rev Cubana Hig Epidemiol 37: 6–12.