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Stream Noise, Hybridization, and Uncoupled Evolution of Call Traits in Two Lineages of Poison Frogs: Oophaga histrionica and Oophaga lehmanni

  • Fernando Vargas-Salinas ,

    vargassalinasf@yahoo.com

    Current address: Programa de Biología, Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, Colombia

    Affiliation Department of Biological Sciences, Universidad de los Andes, Bogotá DC, Colombia

  • Adolfo Amézquita

    Affiliation Department of Biological Sciences, Universidad de los Andes, Bogotá DC, Colombia

Abstract

According to the acoustic adaptation hypothesis, communication signals are evolutionary shaped in a way that minimizes its degradation and maximizes its contrast against the background noise. To compare the importance for call divergence of acoustic adaptation and hybridization, an evolutionary force allegedly promoting phenotypic variation, we compared the mate recognition signal of two species of poison frogs (Oophaga histrionica and O. lehmanni) at five localities: two (one per species) alongside noisy streams, two away from streams, and one interspecific hybrid. We recorded the calls of 47 males and characterized the microgeographic variation in their spectral and temporal features, measuring ambient noise level, body size, and body temperature as covariates. As predicted, frogs living in noisy habitats uttered high frequency calls and, in one species, were much smaller in size. These results support a previously unconsidered role of noise on streams as a selective force promoting an increase in call frequency and pleiotropic effects in body size. Regarding hybrid frogs, their calls overlapped in the signal space with the calls of one of the parental lineages. Our data support acoustic adaptation following two evolutionary routes but do not support the presumed role of hybridization in promoting phenotypic diversity.

Introduction

Species exhibiting intraspecific geographic or microgeographic variation in habitat use offer excellent opportunities to understand the very initial steps of evolutionary divergence in auditory signals [1], [2]. After all, microgeographic variation is probably the minimum amount of evolution that can be detected in nature. Sensory drive is widely recognized as the selective pressure of habitat characteristics on the evolution of communication systems [3], [4]. Since habitats do vary geographically, local adaptations in mate recognition signals may promote reproductive isolation among populations and thereby speciation [5]. For instance, habitat color background is thought to promote the evolution of contrasting colors in dewlaps of visually displaying lizards Anolis [6] and in body parts of birds and cichlid fish [7], [8]. A form of sensory drive known as acoustic adaptation hypothesis (also termed signal structure hypothesis) implies that habitat characteristics such as background noise may evolutionarily shape auditory signals in a way that maximizes their contrast against the background noise [9][11]. The empirical evidence supporting adaptation in acoustic mating signals is surprisingly sparse compared to visual signals [6], [12][16].

Cross-breeding between taxa (hybridization) also can favor the evolution of communication signal diversity because hybrids often exhibit distinctive phenotypes [17], [18]. Distinctive communication traits in the “hybrid” offspring may help to maintain the integrity of hybrid lineages by reducing the chances of back crossbreeding with any parental species [18], [19] which may result in hybrid speciation [20]. Indeed, recent evidence suggests that hybridization can play an unsuspected important role in animal diversification [21][24]. However, hybridization events can also be costly if hybrid offspring is less adapted than parental individuals, thus promoting the evolution of strong premating isolation mechanisms (i.e. reinforcement [17], [25], [26]. Last but not least, hybridization has been also considered as a homogenizing force reversing initial divergence between lineages [21], [27]. Summing up, hybridization has been recognized as a widespread phenomenon in nature for decades [23], [28], but the variety of hybridization effects preclude strong generalizations about its role in the evolution of communication signals diversity.

The role of natural selection on the divergence of auditory communication signals has been compared to the role of other evolutionary forces such as sexual selection and genetic drift [29][32]. However, we are not aware of any attempt to simultaneously contrast the role of natural selection and hybridization in the evolution of an auditory signal. Whereas natural selection is expected to promote gradual divergence in communication signals, hybridization is considered an evolutionary mechanism capable of generating large phenotypic variation in short time intervals compared to natural selection [33], [34]. Thus, where both processes have recently occurred, one should expect either (1) a signal phenotype representing a tradeoff between adaptation and hybridization or (2) uncoupled evolution of signal characteristics, i.e. some signal traits will reflect the effect of natural selection whereas others will just represent the genetic consequences of hybridization.

Among vertebrates, poison frogs (Dendrobatidae) represent an increasingly important model in which to study the evolutionary biology of complex signals and its implications in speciation. They use multimodal (e.g. visual and acoustic) and multicomponent (e.g. color and pattern) communication signals [32], [35], [36] that are heritable ([37], [38], Amézquita, unpublished data) and often exhibit intraspecific geographic variation [32], [39]. Among the mechanisms allegedly promoting geographic variation in their complex signals are divergent female choice preferences, adaptation to local predators, mimetic processes, and genetic drift [32], [39][41]. We expect here to add to this knowledge by testing the simultaneous effect of natural selection represented by stream noise and hybridization in the evolution of an auditory signal.

The frogs Oophaga histrionica and O. lehmanni inhabit tropical wet forests where males usually establish territories, call, and mate near forest gaps and forest edges [42], [43]. At some localities, however, territorial males are heard from riparian forests, where the acoustic environment is strongly dominated by the noise produced by fast flowing streams. Streams produce a continuous broadband low-frequency noise, which should favor the evolution of high frequency calls in frogs as an adaptation that reduces masking interference by abiotic noise [44][46]. Hence, individuals of O. histrionica and O. lehmanni living alongside noisy streams should exhibit higher call frequencies than individuals living away from streams, where the broadband low-frequency noise is significantly lower in intensity. On the other hand, cross-breeding experiments and microsatellite analyses have shown that at least one population currently assigned to O. histrionica resulted from hybridization between O. histrionica and O. lehmanni [47]. Therefore, the hybrid population of O. histrionica could exhibit either intermediate or novel call traits relative to the parental lineages, perhaps distinctive enough to increase the probability of incipient speciation. Our aim here was to estimate the effect of stream noise and hybridization on microgeographic divergence of an acoustic mating signal. To reach this goal, we 1) characterized geographic variation on spectral and temporal traits of the advertisement calls of O. histrionica and O. lehmanni, and then used multivariate analyses to 2) compare each species' calls between individuals occurring at streams and away from them, and 3) compare the calls of a hybrid population against the call of the parental lineages.

Materials and Methods

Ethics statement

Procedures for recordings, capture and handling of live animals in the field were approved by the Colombian Ministry of Environment under the research permit 004 of July 27–2007. The Unidad Administrativa Especial de Parques Nacionales Naturales de Colombia and the Corporación Autónoma Regional del Valle del Cauca CVC gave us permission and logistic support for working in all study localities.

Study species

The poison frogs Oophaga histrionica and O. lehmanni (Dendrobatidae) inhabit tropical wet forests, on the pacific slope of the western Colombian Andes. Whereas O. histrionica is widely distributed between sea level and 1000 m elevation, O. lehmanni is scattered within less than 400 km2, between 550–1010 m elevation [43], [48]. Here we studied three populations currently assigned to O. histrionica and two populations to O. lehmanni, all of them in the Departamento del Valle del Cauca. We publish here the approximate position rather than the precise coordinates of the study sites because both species are heavily trafficked for the international pet market and O. lehmanni (Critically Endangered according to [49]) is almost extinct throughout its formerly known distribution range [50]. One population of each species occurs in riparian habitats (hereafter Oh-Stream, Ol-Stream) where the acoustic environment is highly influenced by high-intensity and low-frequency noise produced by rocky streams. Another population of each species occurs away from fast flowing streams (hereafter Oh-Away, Ol-Away), in less noisy habitats (Fig. 1). The third population of O. histrionica (hereafter Oh-Hybrid) has arisen from natural cross-breeding between the latter two populations of O. histrionica and O. lehmanni [47] and has been found predominantly away from noisy streams (Fig. 1).

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Figure 1. Study sampling scheme.

Calls were recorded from two populations of Oophaga histrionica, two of O. lehmanni and a hybrid population between both species [47], which is currently assigned to O. histrionica (Oh-Hybrid). One population of each species (Oh-Stream, Ol-Stream) occurs along streams where environmental noise (median and range expressed in dB, re 20 µPa) is higher than for three other populations occurring away from streams (Oh-Away, Oh-Hybrid, Ol-Away). Tree topology from [47].

https://doi.org/10.1371/journal.pone.0077545.g001

Recording and analysis of advertisement calls

Most individuals (40/47) were recorded in the field during ten field trips between April 2009 and September 2012; seven were recorded under lab conditions due to significant security concerns in the study area, which has been historically occupied by guerrillas. Calling males were generally found during daytime hours in elevated positions on the forest floor upon fallen trunks, leaves, and abundant leaflitter; in Oh-Stream, males called from the ground and from crevices in rocky areas along the streams. Once a calling male was located, we recorded its advertisement calls by positioning a unidirectional microphone (Sennheiser K6/ME66), connected to a digital recorder (Marantz PMD660), 30–150 cm from a calling male. Immediately after recording we measured male body temperature to the nearest 0.1°C with an infrared thermometer (Oakton model 35629) and captured him to measure body size (snout-vent length, SVL) to the nearest 0.1 mm with a digital caliper. At the end of each recording session, we measured ambient noise level (maximum value during 10 s) at the calling position to the nearest dB (re 20 µPa) with a sound level meter Roline RO-1350.

Digital recordings were analyzed with Raven 1.4 sound analysis software [51]. The terminology and procedures for measuring call traits were based on [52]. From oscillographic representations of the call, we measured pulse cycle duration (pulse duration + interpulse interval duration), call duration, number of pulses per call and then calculated pulse repetition rate; from spectrograms we measured peak frequency as the call frequency with the highest energy content. Because call traits such as peak frequency and pulse cycle duration can vary within a single call in O. histrionica and O. lehmanni, we repeated these measurements in three pulses located at the initial, middle and final portions of a single call and then used the corresponding average values for further analyses. From power spectra (Window: Blackman, DFT: 2048 samples), we measured peak frequency. For each call parameter, the average of measurements taken on three calls per male was used as the smallest unit of statistical analysis.

Statistical analyses

Since sound pressure levels were measured in dB, which represent a logarithmic scale, the calculation of the average values and further statistical analyses were conducted after converting dB values to a linear scale (pressure, Pa). Differences in ambient noise level among populations were tested with an Analysis of Variance ANOVA using significant level of α<0.05. Since the other measured acoustic traits are usually intercorrelated, we reduced redundancy by conducting a principal component analysis (PCA) with Varimax-rotation. Because body temperature may affect calling performance and thereby acoustic trait values, we removed its effect by conducting linear regression on PCAs, and then used the regression residuals as new temperature-independent acoustic traits in subsequent statistical analyses. To estimate the degree of acoustic divergence among the five studied populations we ran a canonical discriminant analysis. To test whether abiotic noise originated from streams was correlated with high call frequency in Oh-Stream and Ol-Stream, we ran an ANOVA. Because part of the call divergence could be merely explained by co-variation in body size, we repeated the ANOVA after removing concomitant variation in male body size [37], [53]. To test for intra-locality relationships between ambient noise level and call characteristics (PCAs after statistically controlling effect of temperature) we used an analysis of covariance (ANCOVA).

Results

As expected, we detected significant non-overlapping differences in environmental noise between the habitats of streamside frogs (Oh-Stream and Ol-Stream) and frogs living away from streams (Oh-Away, Ol-Away, and Oh-Hybrid) (F = 20.546, DF = 4, P<0.001; Fig. 1). Variation in the measured call traits (Table S1) was successfully summarized by three principal components (Table 1) mainly correlated with call frequency (PC1), call duration (PC2), and pulse number (PC3). After removing the effect of temperature, we detected significant microgeographic divergence in spectral (ANOVA, resPC1-Frequency: F = 7.995, P<0.001) and one of the temporal call features (resPC2-Duration: F = 5.786, P = 0.001; PC3: F = 0.799, P = 0.533) (Fig. 2). Raw data of call features for every frog are available on request to authors.

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Figure 2. Geographic variation in advertisement calls among populations of Oophaga histrionica (Oh-Stream, Oh-Away), O. lehmanni (Ol-Away, Ol-Stream), and a hybrid population between both species.

Boxplots represent principal component scores (see Table 1) that summarize co-variation in original call traits after controlling for temperature (left column), or temperature and body size (right column), except for PC3-Pulse number which was not correlated with either variable. Sample size: Oh-Stream  = 9 males, Oh-Away  = 8, Oh-Hybrid  = 8, Ol-Away  = 12, and Ol-Stream  = 10.

https://doi.org/10.1371/journal.pone.0077545.g002

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Table 1. Principal component analysis summarizing variation in advertisement calls of Oophaga histrionica (Oh-Stream, Oh-Away, Oh-Hybrid) and O. lehmanni (Ol-Away, Ol-Stream).

https://doi.org/10.1371/journal.pone.0077545.t001

The first discriminant function (Table 2), mostly related to resPC1-Frequency, explained 58.8% of call variation. It separated very well two populations with high frequency calls (Oh-Stream and Ol-Stream) from three populations with low frequency calls (Oh-Away, Ol-Away and Oh-Hybrid) (Fig. 3A). The second discriminant function, mostly related to resPC2-Duration, explained an additional 37.1% of the variation. Along the corresponding axis, it tended to separate two populations with relatively long calls (Oh-Stream and Oh-Away) from three populations with shorter calls (Ol-Stream, Ol-Away, and Oh-Hybrid). Considering both functions, the calls of hybrid frogs, Oh-Hybrid, overlapped almost completely with the calls of Ol-Away. Since populations differed in average body size (F = 91.52, DF = 4, P<0.001; Fig. 4), we repeated the discriminant analysis after controlling for both temperature and body size effects (Multiple regression, PC1: R2 = 0.431, F = 16,673, P<0.001; PC2: R2 = 0.278, F = 8.453, P = 0.001). As a result (Table 2), one of the riparian populations (Oh-Stream) moved towards the signal spaces of Ol-Away and Oh-Hybrid and overlapped completely within them (Fig. 3B). The other riparian population (Ol-Stream) remained statistically distinguishable from any other population, because of its high frequency calls. There was no intrapopulation relationship between ambient noise level and any of the call characteristics (resPC1-Frequency: Ancova F = 0.572, DF = 1, P = 0.455, N = 40; resPC2-Duration: F = 0.682, DF = 1, P = 0.415; PC3-Pulse number: F = 0.214, DF = 1, P = 0.647).

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Figure 3. Variation in advertisement call among populations.

Discriminant plots of microgeographic differences in advertisement calls of two populations of Oophaga histrionica (Oh-Stream, Oh-Away), two of O. lehmanni (Ol-Away, Ol-Stream), and a hybrid population between both species (Oh-Hybrid). The first discriminant function mainly represents call frequency whereas the second represents call duration (see also Table 2 for values). A: After controlling for covariation in body temperature. B. After controlling for covariation in body temperature and body size. Dots represent recorded males, squares represent the bivariate centroids for each population, and the shadowed ellipses encompass the 95% confidence limit for each centroid. Populations that are significantly different should have non-intersecting ellipses.

https://doi.org/10.1371/journal.pone.0077545.g003

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Figure 4. Geographic variation in body size.

Difference in body size (Snout-vent length, SVL) among populations of Oophaga histrionica (Oh-Stream, Oh-Away), O. lehmanni (Ol-Away, Ol-Stream), and a hybrid population between both species (Oh-Hybrid). Sample size: Oh-Stream  = 9 males, Oh-Away  = 8, Oh-Hybrid  = 8, Ol-Away  = 12, Ol-Stream  = 10.

https://doi.org/10.1371/journal.pone.0077545.g004

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Table 2. Discriminant analyses to predict population membership of male advertisement calls of Oophaga histrionica and O. lehmanni.

https://doi.org/10.1371/journal.pone.0077545.t002

Discussion

Our results demonstrate uncoupled divergence in call traits among the studied populations. Concerning the spectral call traits, populations were grouped independently of their phylogenetic relationship, but according to similarities on their habitat acoustic environment. In contrast, concerning temporal call traits, populations were grouped according to their phylogenetic affinity rather than their acoustic environment. Hybridization did not appear to promote call diversity in our study system.

Supporting our first prediction and the acoustic adaptation hypothesis, males from the two populations occurring along noisy streams (one per species, Oh-Stream and Ol-Stream) produced calls with higher frequency than males living away from streams. The pattern suggests parallel adaptation in both species. Since stream noise contains most of its energy at low frequencies ([44], [45], [54]; see Fig. 5), calling at higher frequencies should increase the signal-to-noise ratio and thereby the probability of being detected and recognized by conespecific receivers [55]. Vegetation complexity is also thought to affect the evolution of auditory signals, e.g. in birds [26], [56]. However, the empirical evidence supporting this effect in anurans is still weak and most comparisons have been made between populations inhabiting strikingly different habitats, such as open and forested areas [57][59]. Although we did not measure habitat complexity, variability between frogs in the microhabitat around calling perches appeared to be much higher than the corresponding differences between populations. In contrast, noise is obviously a more continuous and predictable environmental pressure, especially where frogs are restricted to narrow forest bands along the streams.

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Figure 5. Power spectra of the abiotic noise on streams.

Symbols denote mean values (squares) and standard deviations based on eight recordings (Window: Blackman, DFT: 2048 samples).

https://doi.org/10.1371/journal.pone.0077545.g005

An upward shift in call frequency may have been evolutionary attained in two ways. First, call frequency is largely determined by vocal cord mass, which, in turn, is often correlated with male body size [53], [60]. Thus, stream noise in riparian habitats may actually confer a selective advantage to small males. Males of Oh-Stream are smaller than any other studied male (Fig. 4) and, once we statistically removed the negative effect of body size on call frequency, their calls are not longer distinguishable from the calls of males living away from streams (Fig. 3). Of course, the pleiotropical link between call frequency and body size can confound the effect of selection on either trait [37]. Numerous selective pressures are thought to act mainly on body size and pleiotropically affect call frequency [61][63]. Conversely, selective pressures on detection and recognition of mate recognition signals could be strong enough to promote concomitant changes in body size, given their tremendous importance for fitness [64], [65]. Interestingly, the latter possibility has been very rarely explored [31], [37].

The upward shift in call frequency may also have arisen from morphological variation in the vocal apparatus that is not pleiotropically linked to body size. A shift in call frequency without concomitant variation in body size was observed in O. lehmanni (Ol-Stream). Their calls remained distinguishable in frequency from the calls of any other population even after statistically removing the negative effect of body size (Fig. 3B). A morphological analysis of the frogs' vocal apparatus is beyond the scope of this study; however, previous studies indicate that novel fibrous masses adhered to the vocal cords may effectively increase their mass and thereby lead to the evolution of low frequency calls [64]. Another possibility, much less explored, is the modification of vocal chords' tension without concomitant effects on their mass.

Increasing call frequency implies that the acoustic signal is more locatable by receivers, but it propagates at a shorter distances [37]. It is assumed that signals covering larger distances can increase mating chances to the emissary because it can be detected by a higher number of females. However, it is possible that calls of many frog species are adapted for ease of localization at short distances instead that for long range propagation [65]. In fact, several streamside breeding anurans are characterized by weak calls and/or lack of auditory signals [66], [67]. Increasing signal's call frequency also can imply higher scattering and excess attenuation compared with lower call frequency signals [37]. However, recent evidence indicates that vegetation density affects the temporal rather than the spectral features of anuran calls [68], and studies on anuran assemblages have generally failed to find evidence supporting a role of habitat structure in the evolution of call traits [57], [59], [61], [69].

Our data failed to support the second prediction: hybrid calls were not intermediate between the parental lineages' calls nor were they distinctive enough to increase the probability of incipient speciation. Although both species' calls could be differentiated on the basis of their temporal parameters (call duration, pulses per call), Oh-Hybrid calls overlapped almost completely with Ol-Away calls (Fig. 3). Studies in other taxa have also found that hybrid calls are very similar to those produced by one of the parental species [65], [70]. The absence of distinctive call traits in the hybrid population implies that the hybrid population may well continue back-crossing with one of its parental species [47]. Alternatively, mate recognition might depend upon a combination of auditory signals and visual cues (color pattern) as has been suggested for Oophaga pumilio, a phylogenetically close taxon [40], [71]. This possibility, however, remains to be tested.

Summing up, our data support the hypothesis that stream noise has favored the evolution of high frequency calls in the poison frogs O. histrionica and O. lehmanni on a microgeographic scale. The evolutionary route, however, apparently differed between the species: it implied pleiotropical differences in body size in O. histrionica but not in O. lehmanni. Stream noise appears to be a selective force strong enough to promote microgeographic divergence in calls despite the counteracting effect of genetic flow at the microgeographic scale [47]. The potential for speciation of this environmentally driven signal diversification deserves further study.

Supporting Information

Table S1.

Statistical summary of advertisement call traits, made for the studied populations of poison frogs: Oophaga histrionica (Oh-Stream, Oh-Away), O. lehmanni (Ol-Away, Ol-Stream), and a hybrid population between both species (Oh-Hybrid). Values are Mean ± standard deviation.

https://doi.org/10.1371/journal.pone.0077545.s001

(DOC)

Acknowledgments

For additional support with field and lab logistics, the authors express their gratitude to Germán Corredor (Fundación Zoológico de Cali), the Wildlife Conservation Society (WCS), and the Corporación Autónoma Regional del Valle del Cauca (CVC). Beatriz Velázquez, C. Silva, I. Medina, A. Batista, D. Mejia, J. Velasco, and Carlos Mamián offered invaluable help during field work. Previous version of the manuscript was highly improved by commentaries of the reviewers Peter Narins and Thierry Aubin.

Author Contributions

Conceived and designed the experiments: FVS AA. Analyzed the data: FVS AA. Contributed reagents/materials/analysis tools: FVS AA. Wrote the paper: FVS AA.

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