Urothelial carcinoma shows frequent amplifications at 6p22 and 1q21–24. The main target gene at 6p22 is believed to be E2F3, frequently co-amplified with CDKAL1 and SOX4. There are however reports on 6p22 amplifications that do not include E2F3. Previous analyses have identified frequent aberrations occurring at 1q21–24. However, due to complex rearrangements it has been difficult to identify specific 1q21–24 target regions and genes.
We selected 29 cases with 6p and 37 cases with 1q focal genomic amplifications from 261 cases of urothelial carcinoma analyzed by array-CGH for high resolution zoom-in oligonucleotide array analyses. Genomic analyses were combined with gene expression data and genomic sequence analyses to characterize and fine map 6p22 and 1q21–24 amplifications.
We show that the most frequently amplified gene at 6p22 is SOX4 and that SOX4 can be amplified and overexpressed without the E2F3 or CDKAL1 genes being included in the amplicon. Hence, our data point to SOX4 as an auxiliary amplification target at 6p22. We further show that at least three amplified regions are observed at 1q21–24. Copy number data, combined with gene expression data, highlighted BCL9 and CHD1L as possible targets in the most proximal region and MCL1, SETDB1, and HIF1B as putative targets in the middle region, whereas no obvious targets could be determined in the most distal amplicon. We highlight enrichment of G4 quadruplex sequence motifs and a high number of intraregional sequence duplications, both known to contribute to genomic instability, as prominent features of the 1q21–24 region.
Citation: Eriksson P, Aine M, Sjödahl G, Staaf J, Lindgren D, Höglund M (2013) Detailed Analysis of Focal Chromosome Arm 1q and 6p Amplifications in Urothelial Carcinoma Reveals Complex Genomic Events on 1q, and SOX4 as a Possible Auxiliary Target on 6p. PLoS ONE 8(6): e67222. https://doi.org/10.1371/journal.pone.0067222
Editor: Jörg D. Hoheisel, Deutsches Krebsforschungszentrum, Germany
Received: September 20, 2012; Accepted: May 20, 2013; Published: June 18, 2013
Copyright: © 2013 Eriksson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work supported by The Swedish Cancer Society, http://www.vr.se/(grant number 10 0517) The Swedish Research Council, (www.cancerfonden.se/) (grant number 2011-17816-83790-87), The Crafoord Foundation, (http://www.crafoord.se/vetenskaplig_forskning.asp), The Gunnar Nilsson Foundation, (http://www.cancerstiftelsen.com/), The Medical Faculty of Lund University Hospital, (http://www.med.lu.se/), and The Swedish Society for Medical Research, (http://www.ssmf.se/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Urothelial carcinoma (UC) is the sixth most common malignancy and the fourth most common cancer among males. UC originates from the epithelial cells of the inner lining of the bladder wall. Most tumors (70%) are papillary and confined to the urothelial mucosa (stage Ta) or to the lamina propria (stage T1), whereas the remaining are muscle invasive (T2–T4). Most Ta tumors are of low grade, rarely progress, and are associated with a favorable prognosis whereas high grade Ta (TaG3) and T1 tumors have a significant risk of tumor progression. UC has been studied by gene expression profiling – and recently Lindgren et al.  classified UC based on gene expression and genomic alterations. Several genes are known to be mutated in UC, of which activating mutations in FGFR3 and inactivating mutations in TP53 are the most frequent. Accumulated data has shown that FGFR3 mutations are characteristic for low grade and low stage tumors whereas TP53 mutations are characteristic for invasive tumors –. Apart from gene mutations, cytogenetic studies have revealed several recurring chromosomal changes and comparative genome hybridization (CGH) methods have corroborated many of these findings, but also defined several recurrent high level amplifications and deletions , –. Key findings of these investigations are frequent losses of chromosome arms 9p and 9q, and frequent amplifications on 6p and 1q. Losses of chromosome 9, and of 9p in particular, are highly characteristic for low stage and low grade UC. Deletions affecting 9p are commonly attributed to loss of the tumor suppressor gene CDKN2A at 9p21 . High-level amplifications on 6p are commonly localized to the 6p22.3 region and are frequent in advanced stage UC. The genes most frequently encompassed by 6p22 amplifications are E2F3, CDKAL1, and SOX4. Amplifications at 1q21–24 are frequent but heterogeneous. The heterogeneity of 1q21–24 amplifications has most likely precluded the identification of bona fide target genes. In order to clarify some of the genomic features of 6p and 1q amplifications in UC we have applied high-resolution array CGH focused at regions commonly altered in UC combined with gene expression analysis.
Materials and Methods
Patients and tumor tissue samples
Samples were obtained by cold-cup biopsies from the exophytic part of the bladder tumor from patients undergoing transurethral resection at hospitals of the Southern Healthcare Region of Sweden. Pathological evaluation was based on WHO 1999. Written informed consent was obtained from all patients and the study was approved by the Local Ethical Committee at Lund University. Using previous information on genomic imbalances in 261 cases of urothelial carcinoma , , , , 68 cases were selected based on the presence of focal genomic aberrations. Among the samples, 48 harbored focal genomic alterations either at 6p22, at 1q21–24, or both (Table S1). Alterations at 6p22 and 1q21–24 co-occurred in 18 samples. Alterations of the 6p22 and 1q21–24 region alone occurred in 11 and 19 samples, respectively, for a total of 29 samples with 6p22 alterations and 37 samples with 1q21–24 alteration. The 20 remaining samples lacking aberrations at 6p or 1q were selected based on the presence of other commonly recurring genomic alterations. Gene expression data was available for 212 of the original 261 samples, and for 58 out of the 68 samples selected for zoom-in analyses .
A custom design 180 k Agilent G3 Sureprint (Agilent Technologies, Santa Clara, CA, USA) array was used, which covers the genome and contains increased probe densities at selected regions of the genome (Table S2). The average probe spacing was 17 bp and between 7000–12000 bp in selected target regions. Target regions were selected based on previous array CGH analyses using a 32 K BAC platform. Tumor sample and male reference DNA (Promega, Madison, WI, USA) were labeled and hybridized to arrays as described . Tumor samples with a low DNA quantity were amplified using the GenomePlex WGA2 amplification kit (Sigma-Aldrich, St Louis, MO, USA) according to manufacturer's protocol with 20–40 ng of input DNA prior to labeling. The reference DNA for these samples was also subjected to whole genome amplification.
Copy number analysis
Raw data was extracted from the scanned images using Agilent Feature Extraction 10.7.3.1 (Agilent Technologies, Santa Clara, CA, USA). The data was filtered from control probes and probes that did not pass Agilent's default "well above background" condition. Remaining probes were corrected for background signal and log 2 ratios (log 2 (Signal sample/Signal reference)) were calculated from the adjusted signal intensities for each array. The log 2 ratios were normalized and centered using popLowess . The log 2 values of replicate probes were merged to their median value. Segmentation was performed on normalized log 2 ratios for each sample using Circular Binary Segmentation (CBS)  (Settings: 10 000 permutations, significance level for accepting change-points, α, set to 0.01, and a minimum of 5 consecutive probes for calling a segment). Gains and losses were called at regions where the segmentation value exceeded a sample adaptive threshold (SAT) . The SAT ranged from 0.15 to 0.59, with a median value of 0.20. Copy number gain frequencies were calculated using segmented data at an individual probe level by dividing the number of times the probe was observed above the SAT with the number of samples investigated. Average copy number gain amplitudes (log 2) were calculated by measuring the summed segmentation line amplitude of each probe above SAT divided by the number of times the probe was observed above the SAT. RefSeq gene locations were downloaded from the UCSC genome browser (GRCh37/HG19 Assembly). MicroRNA (miRNA) data was obtained from miRBase (http://www.mirbase.org, Release 18). Copy number variant (CNV) data generated by Conrad et al.  was used to account for naturally occurring variations. Gene specific copy number was measured as the mean segmentation value spanning each RefSeq gene position. The correlation between gene specific copy number and gene expression levels was determined using Spearman correlation in the 58 samples with matched gene expression, and p-values were FDR corrected to account for multiple testing . The gene expression levels in samples with amplifications were compared to the remainder of the 212 samples where expression data was available using the Mann-Whitney Test, in order to determine whether there was a significant difference in expression levels. Raw and processed data, together with array design and sample annotations, are deposited in the Gene Expression Omnibus (GSE40938).
Breakpoint and sequence element analyses
Breakpoints were called at positions where the segmentation shifts exceed the SAT or occurred above the SAT. Breakpoints were manually curated in selected regions to account for outlier probes. In order to test for an uneven distribution of chromosomal breaks within the 1q and 6p target regions, the observed breakpoint distribution was compared to that of 10000 random permutations in 50 kb windows. Significance levels were determined by rank statistics. Data on repetitive genomic features (LINE, SINE, and LTR) was downloaded from the UCSC genome browser RepeatMasker track . Locations of segmental duplications were obtained from the UCSC genome browser (Duplications of >1000 Bases of Non-RepeatMasked Sequence). G4 quadruplex locations were obtained using the Quadparser algorithm, which identifies d(G3N1–7G3N1–7G3N1–7G3) sequence motifs postulated to fold into a quadruplex structure . LINE, SINE, LTR, and G4 sequence element content was measured in 50 kb non-overlapping windows across the genome. In order to assess the association between element content and breakpoint occurrence, the breakpoint frequency in windows that harbored an above median element content was compared to that of windows with a below median element content. Only regions with array coverage were included, and windows with CNVs were excluded. Fisher's exact test was used to assess the significance of repetitive sequence enrichment in the 1q and 6p amplicon peak regions.
The 6p22 region
Of the 261 cases analyzed by 32 K BAC array-CGH 29 cases showed focal copy number alterations occurring within the 6p22.3 region (Chr6:14.9–24.8 Mb). The frequency plot (Figure 1A) places E2F3 at the slope of the amplification frequency peak, with the most frequently amplified gene being SOX4. When amplified, however, both genes show similar amplification amplitudes (Figure 1B). Although the focal genomic amplifications usually included all three genes (E2F3, CDKAL1, and SOX4), we detected four cases (14%) in which E2F3 was not included in the amplified segments (Figure 2). These four cases showed amplification breakpoints between E2F3 and SOX4: within the CDKAL1 coding region in three cases and in the CDKAL1 promoter region in one. Hence, the only intact amplified gene in these four cases was SOX4. No cases with E2F3 amplification without concomitant SOX4 amplification were found. This strongly argues for SOX4 as an auxiliary target to E2F3 in 6p22.
A) Amplification frequency plot and B) average log 2 ratios for probes when amplified. Tracks for location of CNVs, genes, and microRNAs are given. Genomic positions in Mb (HG19).
Amplification breakpoints occur within the coding region of CDKAL1 in A), B), and C), and within the CDKAL1 promoter region in D).
A total of 213 segmentation shifts indicating chromosomal breaks were identified within the 6p22.3 region (Figure 3). The breaks were binned in 50 kb non-overlapping windows and tested for an uneven distribution within the region. Enrichment of breaks was observed between E2F3 and CDKAL1 (p<1×10−3) and to a lower extent at the proximal side of SOX4 (p<1×10−2). To assess whether sequence elements were associated with breakpoint occurrence, the content of LINE, SINE, LTR, and G4 sequences was measured in 50 kb windows across the genome. The median genome-wide sequence element content per 50 kb window was 19.2% LINE, 10.8% SINE, 7.5% LTR, and 28 bp of G4 motif sequence. Genome-wide, breakpoints occurred preferentially in segments with an above median number of SINE and G4 elements, 1.8 and 1.4 fold higher frequency of breakpoints, respectively (p<3×10−16, Mann-Whitney test), and less frequently in segments enriched for LINE and LTR elements (0.8 and 0.8 fold, p<3×10−16). The 6p22.3 amplicon region showed a significantly higher frequency of SINE sequences but a significantly lower frequency of G4 sequences, compared to the genome as a whole (Table 1). No apparent association between breakpoints in the E2F3-SOX4 region and the presence of the investigated sequence elements was observed (Figure 3).
Breakpoint occurrence within 50 kb non-overlapping windows across the 6p target region. Significance thresholds, red line, p<10−3; blue line, p<10−2, determined by permutations (10000 fold) of breakpoints in the 6p22.3 region (Chr6:14.9–24.8 Mb). Tracks for LINE, SINE, LTR, and G4 element frequencies within 50 kb windows are given, as well as tracks for intraregional sequence duplications, CNVs, and genes. LINE, SINE, and LTR are displayed as percentage of window, while G4 is displayed as the number of base pairs of G4 sequence per window. No intraregional sequence duplications were located within the 6p22.3 peak region. Genomic positions in Mb (HG19).
Correlations between DNA amplification and mRNA levels were found to be high for all genes within the amplified region, except for ID4. MBOAT1 expression followed gene copy levels closely (ρ = 0.66, p<5×10−6) but was not always included in the amplified regions. E2F3 showed strong correlation (ρ = 0.82, p<3×10−16) and the highest mRNA fold-changes. SOX4, the most proximal gene showed a highly significant association between gene copy numbers and gene expression (ρ = 0.59, p<8×10−5), as did CDKAL1 (ρ = 0.78, p<3×10−16). SOX4 was overexpressed in cases where E2F3 was not a part of the amplicon. Hence, both increased E2F3 and SOX4 gene copy numbers are strongly associated with increased mRNA expression (Figure 4A and 4B).
The 212 samples with both gene expression and genomic data were rank ordered based on A) E2F3, B), SOX4 C) CHD1L, D) BCL9, E) MCL1, and F) SETDB1 mRNA expression. Cases with focal genomic amplification of the respective gene are indicated with red. For each gene the difference in gene expression between amplified and non-amplified cases were tested by a Mann-Whitney test. The obtained p-values are indicated in each sub graph.
The 1q21–24 region
Thirty-seven of the 261 cases analyzed by 32 K BAC array-CGH harbored 1q copy number aberrations occurring within a 29 Mb genomic segment (Chr1:143.6–172.3 Mb). Although the high resolution zoom-in array further highlighted the heterogeneity of 1q alterations, three regions emerged as candidates for amplification: amplicon 1 at chr1:143.9–148.5 Mb, amplicon 2 at chr1:149.8–152.9 Mb, and a distal amplicon (amplicon 3) at chr1:159.7–161.7 Mb (Figure 5). These regions appear as concomitant amplifications in most cases: in 17 cases (46%) all three regions were amplified, in 6 cases (16%) amplicon 2 and 3, and in 2 cases (5%) amplicon 1 and 2. In no instance were amplicons 1 and 3 co-amplified without amplification of amplicon 2 (Figure 6). Only amplicon 3 was found amplified as a single unit, seen in 12 cases (34%).
A) Amplification frequency plot and B) average log 2 ratios for probes when amplified. Tracks for location of CNVs, genes, and microRNAs are given. Gray boxes indicate the extension of the three 1q amplicons. Arrows indicate the positions of CHD1L, BCL9, MCL1, ARNT, and SETDB1. Genomic positions in Mb (HG19).
A) Each of the three amplicons amplified to a different extent, with a CNV loss occurring between amplicon 1 and amplicon 2. B) Amplicon 2 MCL1 region amplified. C) Similar event as in A but with varying copy number levels in amplicon 2. D) Amplicon region 2 and 3 amplified independently. E) Amplicon 3 amplified alone. F) Amplicon region 1 and 2 amplified as a single unit.
Amplicon 1, observed in 19 of the 37 cases (51%) with 1q gains, always included the genes BCL9 and CHD1L. A strong correlation between BCL9 and CHD1L mRNA expression and gene copy numbers was also observed, (ρ = 0.63, p<2×10−5, and ρ = 0.53, p<2×10−4 respectively). Cases with amplified BCL9 and CHD1L were highly enriched among the high expressing cases (Figure 4C and 4D). Amplicon 2 showed two possible sub-peaks that occasionally appeared as separate amplifications (Figures 6A, 6B, 6C and 6D). The anti-apoptotic gene MCL1 was amplified in 25 out of the 37 (68%) cases, including one case with MCL1 only. Two additional genes included in the peak region were: ARNT, also known as HIF1B, and SETDB1. ARNT/HIF1B was amplified in 24 (65%) of the cases, while SETDB1 was amplified in 23 (62%) cases. All three genes showed a significant correlation between gene copy numbers and gene expression; MCL1 (ρ = 0.73, p<3×10−16), ARNT/HIF1B (ρ = 0.54, p<3×10−4), and SETDB1 (ρ = 0.64, p<6× 10−6). Cases with MCL1, and SETDB1 amplifications where highly enriched among the high expressing cases (Figure 4E and 4F), as was ARNT/HIF1B (not shown). The third amplicon region harbored copy number aberrations in 35 out of 37 cases (95%). The amplicon region spans approximately 68 genes but the amplification frequency peaks around 25 genes located at chr1:160.84–161.35 Mb (Table S3). Eleven of these genes showed strong association (ρ≥0.55, p<3×10−4) between gene copy number and gene expression (Table 2), including the tight junction adhesion related F11R, the death effector domain containing DEDD, and the transcription factor USF1, as well as four genes associated with mitochondrial functions: PPOX, NDUFS2, TOMM40L, and SDHC.
A total of 599 segmentation shifts indicating chromosomal breaks were detected within the 1q amplification region (Figure 7). One region, located within amplicon 1, showed a strong enrichment for breakpoints (p<10−4). No clear association between the clustering of breakpoints and specific sequence elements could be established. However, compared to the whole genome, the 1q region shows higher frequencies of G4 and SINE elements, and lower frequencies of LTR sequences. Furthermore, the 1q region differed significantly from 6p amplification regions with respect to G4 element content (Table 1). A notable feature of the 1q region is the high frequency of intraregional sequence duplications (Figure 7), particularly within the amplicon 1 segment. Similar occurrences of intraregional sequence duplications were not observed in the 6p region (Figure 3).
A) Breakpoint occurrence within 50 kb non-overlapping windows across the 1q target region. Significance thresholds, red line, p<10−3; blue line, p<10−2, determined by permutation (10000 fold) of breakpoints in the 1q region (Chr1:140.0–184.0 Mb). Tracks for LINE, SINE, LTR, and G4 element frequencies within 50 kb windows are given. LINE, SINE, and LTR are displayed as percentage of window, while G4 is displayed as the number of base pairs of G4 sequence per window. Intraregional sequence duplications are connected with green lines in the DupSeq track. Locations of CNVs and genes are given in individual tracks. Genomic positions in Mb (HG19).
The most frequent genomic copy number gains in UC occur on 6p and 1q. The 6p amplification, mostly seen in high grade tumors, has been extensively studied and E2F3 is believed to be the main target. There are however cases with 6p amplifications that do not cover E2F3 . Aberrations of 1q occur both in low and high grade tumors. However, whereas whole chromosome arm gains are seen in low grade tumors, high grade tumors frequently show complex focal amplifications , . In addition, no bona fide target genes have so far been assigned to the 1q region in UC. To resolve some of these issues we selected 29 cases with 6p22 and 37 cases with 1q21–24 focal amplifications from a series of 261 cases analyzed by 32 K BAC array-CGH for high resolution zoom-in array CGH analyses. The applied zoom-in platform has an approximately ten-fold increase in resolution with a design that makes it possible to identify intragenic breakpoints.
The abundance and the high sequence similarity among repetitive elements make them potential driving factors for genomic instability . Mechanisms suggested to be in operation include un-equal crossing-over and non-allelic homologous recombination repair events –. Both the 6p and the 1q regions contained higher frequencies of SINE elements that may contribute to the nature of the amplifications. Alternative forms of secondary DNA structures have also been linked to genomic instability – such as G4 quadruplexes, formed by guanine-rich sequences that adopt four-stranded secondary DNA structures . Regions rich in G4 sequence motifs have been shown to be enriched for DNA breaks in cancer , something we also observe in the present study. Furthermore, hypomethylation, a common feature of cancer genomes, potentially aids the formation of G4 quadruplex structures . In contrast to 6p, the 1q region showed a high frequency of G4 quadruplex sequence motifs, particularly in the amplicon regions 2 and 3. Amplicon 1, on the other hand, showed a large number of intraregional sequence duplications, a feature that is absent in the 6p region. Hence, our data suggest that the observed heterogeneity of 1q amplifications may be a consequence of an underlying regional instability caused by an accumulation of specific sequence motifs. Regions with similarly high density of regional sequence duplications are also seen in other peri-centromeric regions e.g., in chromosomes 7, 9, and 16.
Several investigations have indicated E2F3 as the major target gene for 6p22 amplifications –. E2F3 has a central role in cell cycle regulation  and the frequent E2F3 amplifications are consistent with the frequent RB1 alterations seen in UC, both affecting the same key transition in cell cycle regulation . Hurst et al.  have pointed to an intimate link between E2F3 and RB1 in UC and we have recently identified an E2F3/RB1 genomic circuit operating in a subset of UCs . In light of this, it is intriguing that E2F3 is not the most frequently amplified gene at 6p22. The finding of 6p22 amplifications not spanning the E2F3 gene, with genomic breaks within the CDKAL1 gene, strongly suggests SOX4 as possible auxiliary target gene within 6p22. Intriguingly, both depletion and overexpression of SOX4 may have unfavorable effects on cell survival , . Recent investigations have reported SOX4 as a part of the pro-apoptotic TP53 pathway in which SOX4 expression is induced during DNA damage and stabilizes TP53 by blocking MDM2-mediated ubiquitination and degradation . This function could explain why SOX4 overexpression has been linked to apoptosis and been associated with better patient survival , . In contrast to these findings, SOX4 has also been reported to have positive effect on cellular survival , . SOX4 expression has been linked to increased proliferation through modulation of β-catenin/TCF activity in TP53 mutated cell lines . In addition, SOX4 expression activates EGFR expression and influences the NOTCH pathway , . Taken together these findings indicate SOX4 as a multifunctional protein that may have a context dependent cellular function. All four cases with SOX4 but not E2F3 amplification harbored TP53 mutations. This leaves the question open whether SOX4 could have oncogenic properties when amplified in TP53 mutated cases of UC. Recent investigations have shown that SOX4 is regulated through rapid protein degradation . This indicates that SOX4 function may, in analogy with TP53, be required or triggered at specific cellular conditions or transitions. As a consequence, SOX4 gene copy number alterations resulting in increased mRNA levels does not necessarily have to result in increased steady state SOX4 protein levels. Accordingly, our attempts to establish a link between SOX4 gene copy numbers and increased protein levels by IHC did not show any convincing results. This does however not exclude an oncogenic potential of the SOX4 protein.
Even though many studies identify 1q amplifications as a frequent event in UC, few studies report on specific target genes. This is probably due to the fact that the 1q target region is large and gene dense, and as a consequence, may harbor several target genes. Furthermore, 1q amplifications are heterogeneous and occur in a large genomic region, spanning more than 29 Mb. At least three regions could be identified based on the copy number frequency profiles in the current study. The most proximal region was amplified in close to 60% of the cases with 1q alterations. This region contains at least two genes with potential tumor promoting characteristics: BCL9 and CHD1L. BCL9 acts as a nuclear component of the Wnt pathway in association with LEF/TCF family members . BCL9 overexpression has been linked to increased tumor cell proliferation, survival, migration, and invasion by enhancing β-catenin-mediated transcriptional activity , . Furthermore BCL9 knock-down tumors show a less aggressive phenotype and result in increased host survival in mouse xenograft models of multiple myeloma and colon carcinoma . Overexpression of CHD1L, also known as ALC1 (amplified in liver cancer 1), has been found to inhibit apoptosis, promote G1/S transition, and promote tissue invasion and metastasis –. Furthermore, CHD1L-transgenic mice develop spontaneous tumors in various organs, including liver, neck, and colon . Hence, increased expression of both BCL9 and CHD1L may have tumor promoting effects. The analysis highlighted three genes within the central amplified region on 1q: MCL1, ARNT/HIF1B, and SETDB1. MCL1 is a member of the BCL2 anti-apoptotic gene family and a part of a commonly amplified region containing at least six additional genes that are altered in several cancer types . siRNA knockdown of MCL1 results in increased apoptosis, clearly indicating MCL1 as a target for amplification . HIF1B forms a hetero-dimer with HIF1A and EPAS1/HIF2A that functions as a transcriptional regulator of the adaptive response to hypoxia , . Adaptation to hypoxic conditions may be a prerequisite for tumor progression and metastasis . A recent large-scale study identified a region spanning from MCL1 to SETDB1, as a key amplified region in malignant melanoma, and suggested SETDB1 as the target gene . This was motivated by the finding that overexpression of SETDB1 in an animal model resulted in accelerated melanoma onset and formation . The SETDB1 gene was, however, not always included in the 1q amplifications in the present cohort of UCs. The best established oncogene of the three genes in the central amplicon is MCL1 . As SOX4, MCL1 protein is rapidly degraded by the proteasome which makes an association between gene copy numbers and protein expression hard to establish . However, cells with MCL1 amplification show a more pronounced response to shRNA knock-down of MCL1 than cells wild-type for the gene . In conclusion our analysis of the 1q and 6p regions highlights intrinsic features of the genome such as repetitive element and G4-sequence content as putative enablers of chromosomal instability. The stark contrast between the 1q and 6p amplification patterns suggests that different mechanisms and selection pressures may dictate the appearance of the respective genomic alterations. Further studies are needed to resolve the question of whether the heterogeneous appearance of the 1q region is the result of complex rearrangements in an unstable region or the result of clonal heterogeneity at the population level.
Stage, Grade, and aberrations. Information on stage, grade and genomic aberrations for the samples included in the study.
Regions of increased array coverage, based on frequently occurring alterations in UC.
Conceived and designed the experiments: JS DL MH. Performed the experiments: PE MA GS. Analyzed the data: PE MA. Contributed reagents/materials/analysis tools: JS DL. Wrote the paper: PE MA GS JS DL MH.
- 1. Sanchez-Carbayo M, Socci ND, Lozano J, Saint F, Cordon-Cardo C (2006) Defining molecular profiles of poor outcome in patients with invasive bladder cancer using oligonucleotide microarrays. J Clin Oncol 24: 778–789.
- 2. Blaveri E, Simko JP, Korkola JE, Brewer JL, Baehner F, et al. (2005) Bladder cancer outcome and subtype classification by gene expression. Clin Cancer Res 11: 4044–4055.
- 3. Lindgren D, Frigyesi A, Gudjonsson S, Sjodahl G, Hallden C, et al. (2010) Combined gene expression and genomic profiling define two intrinsic molecular subtypes of urothelial carcinoma and gene signatures for molecular grading and outcome. Cancer Res 70: 3463–3472.
- 4. Dyrskjot L, Thykjaer T, Kruhoffer M, Jensen JL, Marcussen N, et al. (2003) Identifying distinct classes of bladder carcinoma using microarrays. Nat Genet 33: 90–96.
- 5. Kim WJ, Kim EJ, Kim SK, Kim YJ, Ha YS, et al. (2010) Predictive value of progression-related gene classifier in primary non-muscle invasive bladder cancer. Mol Cancer 9: 3.
- 6. Sjodahl G, Lauss M, Lovgren K, Chebil G, Gudjonsson S, et al.. (2012) A Molecular Taxonomy for Urothelial Carcinoma. Clin Cancer Res.
- 7. Lindgren D, Sjodahl G, Lauss M, Staaf J, Chebil G, et al. (2012) Integrated genomic and gene expression profiling identifies two major genomic circuits in urothelial carcinoma. PLoS One 7: e38863.
- 8. Sjodahl G, Lauss M, Gudjonsson S, Liedberg F, Hallden C, et al. (2011) A systematic study of gene mutations in urothelial carcinoma; inactivating mutations in TSC2 and PIK3R1. PLoS One 6: e18583.
- 9. Billerey C, Chopin D, Aubriot-Lorton MH, Ricol D, Gil Diez de Medina S, et al. (2001) Frequent FGFR3 mutations in papillary non-invasive bladder (pTa) tumors. Am J Pathol 158: 1955–1959.
- 10. Wu XR (2005) Urothelial tumorigenesis: a tale of divergent pathways. Nat Rev Cancer 5: 713–725.
- 11. Fadl-Elmula I (2005) Chromosomal changes in uroepithelial carcinomas. Cell Chromosome 4: 1.
- 12. Hoglund M, Sall T, Heim S, Mitelman F, Mandahl N, et al. (2001) Identification of cytogenetic subgroups and karyotypic pathways in transitional cell carcinoma. Cancer Res 61: 8241–8246.
- 13. Richter J, Jiang F, Gorog JP, Sartorius G, Egenter C, et al. (1997) Marked genetic differences between stage pTa and stage pT1 papillary bladder cancer detected by comparative genomic hybridization. Cancer Res 57: 2860–2864.
- 14. Richter J, Beffa L, Wagner U, Schraml P, Gasser TC, et al. (1998) Patterns of chromosomal imbalances in advanced urinary bladder cancer detected by comparative genomic hybridization. Am J Pathol 153: 1615–1621.
- 15. Zhao J, Richter J, Wagner U, Roth B, Schraml P, et al. (1999) Chromosomal imbalances in noninvasive papillary bladder neoplasms (pTa). Cancer Res 59: 4658–4661.
- 16. Veltman JA, Fridlyand J, Pejavar S, Olshen AB, Korkola JE, et al. (2003) Array-based comparative genomic hybridization for genome-wide screening of DNA copy number in bladder tumors. Cancer Res 63: 2872–2880.
- 17. Blaveri E, Brewer JL, Roydasgupta R, Fridlyand J, DeVries S, et al. (2005) Bladder cancer stage and outcome by array-based comparative genomic hybridization. Clin Cancer Res 11: 7012–7022.
- 18. Heidenblad M, Lindgren D, Jonson T, Liedberg F, Veerla S, et al. (2008) Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors. BMC Med Genomics 1: 3.
- 19. Hurst CD, Platt F, Taylor CF, Knowles MA (2012) Novel tumor subgroups of urothelial carcinoma of the bladder defined by integrated genomic analysis. Clin Cancer Res.
- 20. Knowles MA (1999) Identification of novel bladder tumour suppressor genes. Electrophoresis 20: 269–279.
- 21. Lauss M, Aine M, Sjodahl G, Veerla S, Patschan O, et al.. (2012) DNA methylation analyses of urothelial carcinoma reveal distinct epigenetic subtypes and an association between gene copy number and methylation status. Epigenetics 7.
- 22. Staaf J, Torngren T, Rambech E, Johansson U, Persson C, et al. (2008) Detection and precise mapping of germline rearrangements in BRCA1, BRCA2, MSH2, and MLH1 using zoom-in array comparative genomic hybridization (aCGH). Hum Mutat 29: 555–564.
- 23. Staaf J, Jonsson G, Ringner M, Vallon-Christersson J (2007) Normalization of array-CGH data: influence of copy number imbalances. BMC Genomics 8: 382.
- 24. Venkatraman ES, Olshen AB (2007) A faster circular binary segmentation algorithm for the analysis of array CGH data. Bioinformatics 23: 657–663.
- 25. Conrad DF, Pinto D, Redon R, Feuk L, Gokcumen O, et al. (2010) Origins and functional impact of copy number variation in the human genome. Nature 464: 704–712.
- 26. Benjamini Y, Hochberg Y (1995) Controlling the False Discovery Rate - a Practical and Powerful Approach to Multiple Testing. Journal of the Royal Statistical Society Series B-Methodological 57: 289–300.
- 27. Fujita PA, Rhead B, Zweig AS, Hinrichs AS, Karolchik D, et al. (2011) The UCSC Genome Browser database: update 2011. Nucleic Acids Res 39: D876–882.
- 28. Huppert JL, Balasubramanian S (2005) Prevalence of quadruplexes in the human genome. Nucleic Acids Res 33: 2908–2916.
- 29. Shen MR, Batzer MA, Deininger PL (1991) Evolution of the master Alu gene(s). J Mol Evol 33: 311–320.
- 30. Hastings PJ, Lupski JR, Rosenberg SM, Ira G (2009) Mechanisms of change in gene copy number. Nat Rev Genet 10: 551–564.
- 31. Belancio VP, Roy-Engel AM, Deininger PL (2010) All y'all need to know 'bout retroelements in cancer. Semin Cancer Biol 20: 200–210.
- 32. Konkel MK, Batzer MA (2010) A mobile threat to genome stability: The impact of non-LTR retrotransposons upon the human genome. Semin Cancer Biol 20: 211–221.
- 33. Bailey JA, Liu G, Eichler EE (2003) An Alu transposition model for the origin and expansion of human segmental duplications. Am J Hum Genet 73: 823–834.
- 34. Wang G, Christensen LA, Vasquez KM (2006) Z-DNA-forming sequences generate large-scale deletions in mammalian cells. Proc Natl Acad Sci U S A 103: 2677–2682.
- 35. Wang G, Vasquez KM (2004) Naturally occurring H-DNA-forming sequences are mutagenic in mammalian cells. Proc Natl Acad Sci U S A 101: 13448–13453.
- 36. Zhao J, Bacolla A, Wang G, Vasquez KM (2010) Non-B DNA structure-induced genetic instability and evolution. Cell Mol Life Sci 67: 43–62.
- 37. Boan F, Gomez-Marquez J (2010) In vitro recombination mediated by G-quadruplexes. Chembiochem 11: 331–334.
- 38. De S, Michor F (2011) DNA secondary structures and epigenetic determinants of cancer genome evolution. Nat Struct Mol Biol 18: 950–955.
- 39. Sen D, Gilbert W (1988) Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosis. Nature 334: 364–366.
- 40. Hurst CD, Tomlinson DC, Williams SV, Platt FM, Knowles MA (2008) Inactivation of the Rb pathway and overexpression of both isoforms of E2F3 are obligate events in bladder tumours with 6p22 amplification. Oncogene 27: 2716–2727.
- 41. Oeggerli M, Tomovska S, Schraml P, Calvano-Forte D, Schafroth S, et al. (2004) E2F3 amplification and overexpression is associated with invasive tumor growth and rapid tumor cell proliferation in urinary bladder cancer. Oncogene 23: 5616–5623.
- 42. Feber A, Clark J, Goodwin G, Dodson AR, Smith PH, et al. (2004) Amplification and overexpression of E2F3 in human bladder cancer. Oncogene 23: 1627–1630.
- 43. Olsson AY, Feber A, Edwards S, Te Poele R, Giddings I, et al. (2007) Role of E2F3 expression in modulating cellular proliferation rate in human bladder and prostate cancer cells. Oncogene 26: 1028–1037.
- 44. Oeggerli M, Schraml P, Ruiz C, Bloch M, Novotny H, et al. (2006) E2F3 is the main target gene of the 6p22 amplicon with high specificity for human bladder cancer. Oncogene 25: 6538–6543.
- 45. Chen HZ, Tsai SY, Leone G (2009) Emerging roles of E2Fs in cancer: an exit from cell cycle control. Nat Rev Cancer 9: 785–797.
- 46. Knowles MA (2001) What we could do now: molecular pathology of bladder cancer. Mol Pathol 54: 215–221.
- 47. Aaboe M, Birkenkamp-Demtroder K, Wiuf C, Sorensen FB, Thykjaer T, et al. (2006) SOX4 expression in bladder carcinoma: clinical aspects and in vitro functional characterization. Cancer Res 66: 3434–3442.
- 48. Pramoonjago P, Baras AS, Moskaluk CA (2006) Knockdown of Sox4 expression by RNAi induces apoptosis in ACC3 cells. Oncogene 25: 5626–5639.
- 49. Pan X, Zhao J, Zhang WN, Li HY, Mu R, et al. (2009) Induction of SOX4 by DNA damage is critical for p53 stabilization and function. Proc Natl Acad Sci U S A 106: 3788–3793.
- 50. de Bont JM, Kros JM, Passier MM, Reddingius RE, Sillevis Smitt PA, et al. (2008) Differential expression and prognostic significance of SOX genes in pediatric medulloblastoma and ependymoma identified by microarray analysis. Neuro Oncol 10: 648–660.
- 51. Liu P, Ramachandran S, Ali Seyed M, Scharer CD, Laycock N, et al. (2006) Sex-determining region Y box 4 is a transforming oncogene in human prostate cancer cells. Cancer Res 66: 4011–4019.
- 52. Sinner D, Kordich JJ, Spence JR, Opoka R, Rankin S, et al. (2007) Sox17 and Sox4 differentially regulate beta-catenin/T-cell factor activity and proliferation of colon carcinoma cells. Mol Cell Biol 27: 7802–7815.
- 53. Scharer CD, McCabe CD, Ali-Seyed M, Berger MF, Bulyk ML, et al. (2009) Genome-wide promoter analysis of the SOX4 transcriptional network in prostate cancer cells. Cancer Res 69: 709–717.
- 54. Beekman JM, Vervoort SJ, Dekkers F, van Vessem ME, Vendelbosch S, et al. (2012) Syntenin-mediated regulation of Sox4 proteasomal degradation modulates transcriptional output. Oncogene 31: 2668–2679.
- 55. Kramps T, Peter O, Brunner E, Nellen D, Froesch B, et al. (2002) Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex. Cell 109: 47–60.
- 56. Mani M, Carrasco DE, Zhang Y, Takada K, Gatt ME, et al. (2009) BCL9 promotes tumor progression by conferring enhanced proliferative, metastatic, and angiogenic properties to cancer cells. Cancer Res 69: 7577–7586.
- 57. Deka J, Wiedemann N, Anderle P, Murphy-Seiler F, Bultinck J, et al. (2010) Bcl9/Bcl9l are critical for Wnt-mediated regulation of stem cell traits in colon epithelium and adenocarcinomas. Cancer Res 70: 6619–6628.
- 58. Chen L, Chan TH, Guan XY (2010) Chromosome 1q21 amplification and oncogenes in hepatocellular carcinoma. Acta Pharmacol Sin 31: 1165–1171.
- 59. Chen L, Chan TH, Yuan YF, Hu L, Huang J, et al. (2010) CHD1L promotes hepatocellular carcinoma progression and metastasis in mice and is associated with these processes in human patients. J Clin Invest 120: 1178–1191.
- 60. Ma NF, Hu L, Fung JM, Xie D, Zheng BJ, et al. (2008) Isolation and characterization of a novel oncogene, amplified in liver cancer 1, within a commonly amplified region at 1q21 in hepatocellular carcinoma. Hepatology 47: 503–510.
- 61. Chen M, Huang JD, Hu L, Zheng BJ, Chen L, et al. (2009) Transgenic CHD1L expression in mouse induces spontaneous tumors. PLoS One 4: e6727.
- 62. Beroukhim R, Mermel CH, Porter D, Wei G, Raychaudhuri S, et al. (2010) The landscape of somatic copy-number alteration across human cancers. Nature 463: 899–905.
- 63. Wang GL, Jiang BH, Rue EA, Semenza GL (1995) Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci U S A 92: 5510–5514.
- 64. Tian H, McKnight SL, Russell DW (1997) Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells. Genes Dev 11: 72–82.
- 65. Denko NC (2008) Hypoxia, HIF1 and glucose metabolism in the solid tumour. Nat Rev Cancer 8: 705–713.
- 66. Macgregor S, Montgomery GW, Liu JZ, Zhao ZZ, Henders AK, et al. (2011) Genome-wide association study identifies a new melanoma susceptibility locus at 1q21.3. Nat Genet 43: 1114–1118.
- 67. Ceol CJ, Houvras Y, Jane-Valbuena J, Bilodeau S, Orlando DA, et al. (2011) The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset. Nature 471: 513–517.
- 68. Schwickart M, Huang X, Lill JR, Liu J, Ferrando R, et al. (2010) Deubiquitinase USP9× stabilizes MCL1 and promotes tumour cell survival. Nature 463: 103–107.