In a rapidly changing biosphere, approaches to understanding the ecology and evolution of forest species will be critical to predict and mitigate the effects of anthropogenic global change on forest ecosystems. Utilizing 26 forest species in a factorial experiment with two levels each of atmospheric CO2 and soil nitrogen, we examined the hypothesis that phylogeny would influence plant performance in response to elevated CO2 and nitrogen fertilization. We found highly idiosyncratic responses at the species level. However, significant, among-genetic lineage responses were present across a molecularly determined phylogeny, indicating that past evolutionary history may have an important role in the response of whole genetic lineages to future global change. These data imply that some genetic lineages will perform well and that others will not, depending upon the environmental context.
Citation: Senior JK, Schweitzer JA, O’Reilly-Wapstra J, Chapman SK, Steane D, Langley A, et al. (2013) Phylogenetic Responses of Forest Trees to Global Change. PLoS ONE 8(4): e60088. https://doi.org/10.1371/journal.pone.0060088
Editor: Han YH. Chen, Lakehead University, Canada
Received: December 4, 2012; Accepted: February 21, 2013; Published: April 4, 2013
Copyright: © 2013 Senior et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: These authors have no support or funding to report.
Competing interests: The authors have declared that no competing interests exist.
Elevated carbon dioxide (CO2) and nitrogen (N) availability are expected to have important consequences for forest ecosystem dynamics –, where soil N scarcity may limit the CO2 fertilisation effect , . Atmospheric CO2 concentrations are expected to double by the turn of the century , while anthropogenic N fixation rates have already doubled preindustrial rates , . Tree species have vital roles in carbon (C) and N cycling and are expected to act as important sinks for anthropogenic CO2 emissions . As the dominant constituents of forest ecosystems, tree species also have important extended community effects such as plant-plant and plant-herbivore interactions , . It is, therefore, vital that we understand the consequences of anthropogenic CO2 and N fertilisation on tree species to predict future impacts of these important global change factors on forest ecosystems.
A recent direction in global climate change research is the utilization of phylogenetics to better understand and predict the impacts of global change –. Closely related taxa have the potential to respond in a similar manner to global environmental changes, due to shared evolutionary histories, genetic background, and phenotypic traits. Thus, taking phylogeny into account may provide generality that is more appropriate for modelling the impacts of large-scale global climate change than generalising across species that share fundamental niches. For example, Davis et al.  assessed the flowering time of plant clades occurring in both the United States and the United Kingdom and found that phenological responses to global climate change were shared within clades. Similar trends are likely to occur in the responses of other plant traits to other large-scale perturbation. For example, the magnitude of CO2-induced increases in biomass may vary much more within functional types (e.g. herbaceous vs woody species) than among them  though consistent differences in response may arise when functional groups align with major phylogenetic differences such as gymnosperms vs. angiosperms . However, few studies utilise an explicit phylogenetic framework (see , –) to assess the importance of phylogeny. Such an approach is important as the differences amongst plant functional groups that are currently being explored in global change studies, likely represent the evolutionary consequences of phylogenetic divergence –. Examining phylogenetic responses to climate change may capture a broader range of variation  among taxa for better understanding how plants can respond to increasing CO2, N or other environmental factors.
If a phylogenetic approach is useful to understanding the consequences of global change, Eucalyptus represents a model genus in which to test it. Eucalyptus is a globally important forest plantation species that is planted worldwide and is the dominant genus in many Australian ecosystems, occurring in subalpine woodlands, cool and warm temperate forests, rainforests and tropical savannahs . Having evolved under a large range of climatic and edaphic conditions the genus is also highly diverse with over 700 species displaying a wide range of growth forms, from giant forest to dwarf coastal trees and stunted, multi-stemmed, “mallee” forms in semi-arid areas . This genus is of great ecological and economic importance, yet relatively few studies have been concerned with the effects of climate change on eucalypt forests in native or non-native habitats around the world. Studies to date have found that eucalypt species are responsive to elevated CO2 and N fertilization but responses differ in direction and magnitude, with negative, neutral and positive growth responses documented –. Consequently, the response of eucalypt species to elevated CO2 and N fertilization may not be as general as expected and a phylogenetic approach may be useful to better inform responses to global change.
On the island of Tasmania (Australia), there are 29 species of Eucalyptus that occur in a range of habitats from coastal wet and dry forests to alpine environments. The species belong to the two main subgenera of Eucalyptus (i.e., subgenus Symphyomyrtus and subgenus Eucalyptus; ), but lower taxonomic classifications are unresolved, with a number of authors grouping species in different ways , , . In this paper we follow the classification of Brooker  in which the classification of eucalypt species is based predominantly on morphological traits, including bark, leaf, floral and fruit morphologies, and is largely supported by the available molecular data , .
Using 26 of the 29 species of Eucalyptus found in Tasmania, we used a phylogenetic approach to better understand plant performance in response to the global change factors of elevated CO2 and soil N fertilization. All species were exposed to factorial treatments of ambient and elevated CO2 and low and high soil N concentrations, where it was hypothesised that plant performance of closely related species to these two global change factors would be similar. Specifically, we hypothesized that eucalypt species would respond differentially to elevated CO2 and N fertilization based on past, shared evolutionary history. We found that; 1) individual species’ growth responses are largely idiosyncratic, however, 2) when species were nested within genetic lineages, species within a particular genetic lineage shared similar responses to elevated CO2 and high soil N concentrations, significantly differing from other genetic lineages. Utilising a phylogenetic approach may, therefore, provide a potential framework by which the responses of individual forest species to global climate change may be generalized across groups of closely related species. These results overall suggest that there may be phylogenetic “winners” and “losers” in response to global change factors based on past evolutionary dynamics and/or history.
Seed of 26 native Tasmanian eucalypt species was purchased from Forestry Tasmania (http://www.forestrytas.com.au/); E. archeri, E. morrisbyi and E. coccifera, were not included in the study because seed was not available. Seed of each species was obtained from one to six individual maternal trees from a single population. To enhance germination, the seed of each species was vernalised before sowing. Seed of each species was folded in paper towel, wrapped in cheese cloth and soaked in a solution of water containing a drop of dishwashing detergent overnight. Each seed bundle was then squeezed the following morning to remove excess water and refrigerated for 30 days at 4°C. After this period, the seed of each species was dispersed on the surface of 26 separate trays filled with commercial potting mix, which consisted of eight parts composted fine pine bark and three parts coarse river sand with added macro- and micro-nutrients from Nutricote Grey (Langley Australia Pty Ltd., Welshpool WA), which included N, phosphorus (P) and potassium (K) in the weight ratio of 19∶2.6∶10, at a rate of 3 kg/m−3. The surface of the potting mix was then disturbed gently and covered with vermiculite for water retention. The trays received a daily soaking of water, in equal volumes, in a greenhouse while seeds germinated. Germinants were grown for three weeks until the majority of seedlings of each species had developed the first pair of true leaves and were uniform in size.
Phylogenetic Framework for Tasmanian Eucalypts
A phylogenetic framework for this study was devised using the classification of Brooker  with adjustments using recent molecular genetic information on the subgenera Symphyomyrtus  and Eucalyptus , . Hence, we have devised a recent, genetically informed taxonomical classification. The Tasmanian species belonging to subgenus Eucalyptus were placed in Genetic Lineage 1 (GL1). The four Tasmanian species of series Foveolatae (E. barberi, E. brookeriana, E. ovata, and E. rodwayi) formed Genetic Lineage 2 (GL2); Tasmanian endemics belonging to series Orbiculares and Semiunicolores formed GL3. The remaining (non-endemic) species of subgenus Symphyomyrtus (E. globulus, series Globulares; E. perriniana, series Orbiculares; E. viminalis, E. rubida and E. dalrympleana, series Viminales) were place in GL4. These genetic lineages were used to conduct nested analyses to test for the effects of phylogenetic group and subgenus (Table 1).
Further support for these groupings was found in broad analyses of all eucalypt subgenera (that did not include all the Tasmanian species that were included in this study; , ) that used a relatively new molecular marker called Diversity Arrays Technology (DArT; ). In this study, we used DArT to check the genetic integrity of the groupings that we defined and found that genotyping supported the genetic lineages (see ).
Elevated CO2 and Soil N Fertilization Study
To determine responses to the global change factors of elevated CO2 and soil N fertilization, eucalypt seedlings were grown under all factorial combinations of ambient and elevated CO2 and high and low soil N fertilization. Twelve seedlings of each species were transplanted into forestry tubes filled with the same commercial potting mix (described above). These twelve seedlings of each species were divided randomly into three replicates for each of the four factorial treatments of CO2 (ambient or elevated) and soil N (low and high). The seedlings for each level of CO2 were then placed randomly, via random number generation, into forestry tube racks.
Seedling racks for each CO2 treatment were placed randomly into separate, air-tight, controlled greenhouse chambers maintained at 23°C with a natural photoperiod; half of which were then fertilized with N at the soil surface (details below). The CO2 treatments and their respective seedlings were exchanged between two greenhouse chambers each week; greenhouse chamber effects were avoided further by moving the CO2 tanks and regulator as well as monitoring CO2 concentrations with an infra-red gas analyser (IRGA) device (LiCor 6200, LiCor Inc., Lincoln, NE, USA). The forestry tube racks were also repositioned randomly during these periods to avoid seedling positional effects in the greenhouse. In the elevated CO2 treatment carbon dioxide was elevated to 720 ppm using a CO2 control unit (Thermoline Scientific Equipment, Smithfield, Australia) and compressed CO2. The low CO2 treatment was maintained at ambient CO2 (∼400 ppm) and monitored frequently for leakage of CO2 from the neighbouring high CO2 chamber with the LiCor (none occurred). Seedlings in each treatment were watered on a daily basis until the eighth week of the study, at which time water was applied as needed. At the same time that the CO2 treatments were initiated, pellets of urea at an approximate concentration of 30 kg N ha−1, were applied each month to the high N treatment seedlings to replicate the approximate N addition of forestry practices that alleviates N limitation .
At the end of the experiment (approximately 5 months later; before seedlings became root bound) each seedling was harvested destructively by carefully removing each seedling, gently shaking off as much soil as possible, and severing the aboveground from the belowground biomass at the root collar. The belowground biomass of each individual of each species was sealed in separate plastic bags after collection and the aboveground biomass was placed in separate brown paper bags. The belowground biomass was refrigerated (6°C) and the aboveground biomass was stored at ambient conditions until oven-drying (at the end of the harvest). The aboveground biomass samples were oven-dried for 48 hours at 60°C and then weighed (g). The belowground biomass of each seedling was carefully rinsed, separately, over 2 and 0.5 mm sieves to remove as much soil as possible whilst retaining as much of the fine root biomass as possible. The washed belowground biomass samples were then oven-dried for 48 hours at 60°C and weighed (g). Oven-dried belowground biomass was divided by aboveground biomass to yield root:shoot and above- and belowground biomass was summed to determine total biomass.
All statistical analyses were conducted using the statistical package SAS (version 9.2, SAS Institute Inc., Cary USA). Other traits were measured (height, leaf biomass, leaf area and relative growth rate) but were not included in the analysis due to strong inter-correlations with all traits (R2>0.7; data not presented). Separate analyses were run for each phylogenetic level to maintain statistical power. The responses of seedling aboveground biomass, belowground biomass, total biomass and root:shoot to elevated CO2 and soil N fertilization were analysed to determine how each eucalypt species responded to these environmental variables. Due to mortalities in the greenhouse, whole treatment groups within particular species were absent, making analysis of these species impossible. Consequently, these species were removed from the analysis. There was a highly significant effect of subgenus on species mortality (P<0.001), where 54 seedlings were lost from subgenus Eucalyptus and 31 from subgenus Symphyomyrtus. Five species were removed from subgenus Eucalyptus (GL1) and one species was removed from subgenus Symphyomyrtus (GL4) due to the death of whole treatments (n = 3). However, no significant interactions between mortality and environmental treatments were present. General linear models were used to analyse species, CO2 (2 levels of treatment, ambient and elevated) and N (2 levels of treatment, low and high) effects for each morphological variable (total biomass, aboveground biomass, belowground biomass, and root:shoot; PROC GLM). All interaction terms were included; species × CO2, species × N, CO2 × N and species × CO2 × N, where all main effects and interaction terms were treated as fixed effects. Data were tested for the assumptions of normality and homoscedasticity and appropriate transformations were applied to meet the Shapiro-Wilk test when required. Diagnostic graphical representations were also checked for normality and homoscedasticity. Aboveground, belowground and total biomass data were square root transformed while root:shoot data were power transformed (0.3). A power transformation was applied when log or square-root transformed data did not satisfy the assumptions of normality and homoscedasticity.
To test for phylogenetic patterns in the responses of the eucalypt species, mixed models were conducted in SAS (PROC MIXED). Models tested genetic lineage (four genetic lineages), CO2 (two levels of treatment, ambient and elevated) and N fertilization (two levels of treatment, low and high) effects for each variable (total biomass, aboveground biomass, belowground biomass and root:shoot). These main effects were fixed effects while species(genetic lineage) was used as a random term to test the genetic lineage effect. All interaction terms were also included as fixed effects; genetic lineage × CO2, genetic lineage × N, CO2 × N and genetic lineage × CO2 × N.
To test for the effects of subgenus, CO2 and N fertilization, mixed models were used to test for subgenus (two subgenera; Symphyomyrtus and Eucalyptus), CO2 (two levels of treatment, ambient and elevated) and soil N fertilization (two levels of treatment, low and high) effects for each variable (aboveground biomass, belowground biomass, total biomass and root:shoot). These main effects were treated as fixed effects while species(subgenus) was used as a random term to test the subgenus effect. Species(subgenus) was chosen over genetic lineage(subgenus) as this random term conserves a larger proportion of variation. All interaction terms were included; subgenus×CO2, subgenus×N, CO2×N and subgenus×CO2×N as fixed effects.
There was a three-way interaction between CO2, N fertilization and species for aboveground biomass, indicating that the response of plant species to the combination of CO2 and soil N fertilization is variable (Table 2; Figure 1A, B). For example, N addition resulted in a nearly three-fold increase in the aboveground biomass of E. dalrympleana under ambient CO2, whereas under elevated CO2, N addition had less impact, resulting only in a 12% increase in aboveground biomass. In contrast, N addition under ambient CO2 resulted in a 78% decrease in the aboveground biomass of E. ovata, but a large, 3.5-fold, increase under elevated CO2.
Interaction plots of least squares means (± standard error for C–F) of the aboveground biomass of species (A and B), genetic lineages (C and D) and subgenera (E and F) with control soil N and high soil N (30 kg ha−1 of N added) under both ambient (left panels) and elevated CO2 (right panels). Standard errors are not presented on the species panels (A–B) due to space constraints. Each colour and line (solid/dashed) combination represents each species, genetic lineage or subgenus analysed, which are represented in the respective legends.
Similar to aboveground biomass there was also a three-way interaction for belowground biomass. Species belowground biomass displayed a variety of responses to elevated CO2 and soil N, whereby species responded differently to soil N availability depending on the concentration of CO2 (Figure 2A, B). For example, under ambient CO2, N addition resulted in a six-fold decrease in the belowground biomass of E. barberi whereas under elevated CO2, N addition resulted in a 248% increase in belowground biomass. In contrast, N addition under ambient CO2 resulted in a 252% increase in the belowground biomass of E. cordata but a decrease of 48% under elevated CO2. It is clear that individual species responses to the treatment factors were highly variable.
Interaction plots of least squares means (± standard error for C–F) of the belowground biomass of species (A and B), genetic lineages (C and D) and subgenera (E and F) with control N and 30 kg ha−1 of N added (High N) under both ambient (left panels) and elevated CO2 (right panels). Standard errors are not presented on the species panels (A–B) due to space constraints. Each colour and line (solid/dashed) combination represents each species, genetic lineage or subgenus.
Similar to the species level analysis, a three-way interaction of genetic lineage, CO2 and soil N was identified in both above- and belowground biomass, indicating the importance of evolutionary history in response to these climate change factors. Both the above- and belowground biomass of genetic lineages responded differently to N availability depending on the concentration of CO2 (Table 3; Figure 1C, D; Figure 2C, D). Under ambient CO2, above- and belowground responses by genetic lineage did not significantly differ, regardless of N availability. However, under elevated CO2, the response of GL2 to N addition significantly differed from other genetic lineages, with large increases in above- and belowground biomass of 213% and 166%, respectively. These results indicate that the response of genetic lineages to CO2 and N are highly variable, but they also show that closely related species respond similarly, providing significantly more predictive ability than individual species responses.
Finally, unlike the genetic lineage level comparison, at the broadest phylogenetic level (subgenus), mixed models found no significant interactive effects of subgenus, CO2 and N in seedling morphological responses (Figure 1E, F; Figure 2E, F), but there were, however, main effects (Table 4). Surprisingly, no significant effects of subgenus, CO2 or N were revealed in aboveground biomass. However, there was a significant effect of subgenus on belowground biomass and root:shoot, whereby species in the subgenus Symphyomyrtus had a significantly larger belowground biomass (143%) and root:shoot (33%) than species from subgenus Eucalyptus. Significant main effects of CO2 were also found in belowground biomass and root:shoot indicating that the two subgenera responded to elevated CO2 in a similar manner.
Independently, elevated CO2 or soil N fertilization generally enhances plant growth (e.g. , , , , ) and two-way interactions between CO2 and N availability are also commonly reported in the literature , where N availability is expected to constrain the CO2-induced stimulation of plant growth . These interactions have been observed across a broad range of tree species from different families and ecological contexts , , , , where studies suggest that tree species allocate C to belowground sinks to alleviate N limitation . However in the present study, species displayed considerable variation in root:shoot in response to CO2 concentration. Variable responses in biomass allocation to elevated CO2 may aid in explaining the variable responses of species to both CO2 and N fertilization. Species that display inherently higher proportions of belowground biomass may more effectively exploit soil N and, therefore, respond more strongly to elevated CO2 . Evolutionary history and local adaptation are likely to be drivers of these differential responses, indicating the presence of evolutionary trade-offs  in the responsiveness of species to either elevated CO2 or soil N. A number of species did not appear to respond to elevated CO2 and N fertilization whereas, due to variability in traits (e.g. larger root:shoot ), others did respond to these factors, either singularly or in combination (interactive effects).
The large variation among species responses is likely to complicate our general understanding of the impacts of global change. For example, Spinnler et al.  found differential impacts of elevated CO2 on tree species within model spruce-beech (Fagus sylvatica-Picea abies) ecosystems growing on either acidic or calcareous soils with either standard or increased nutrient availability. The biomass of P. abies was enhanced by elevated CO2 on both soils, whereas F. sylvatica only responded to elevated CO2 when grown in acidic soil. In this case, the biomass of F. sylvatica decreased by 10% with added nutrients and by a further 24% with no added nutrients in response to elevated CO2. The results of our study indicate that variable species responses to global change may in fact lead to changes in ecosystem dynamics. The highly variable responses among species within this study suggest that species shifts in eucalypt dominated communities could occur under elevated CO2 and changes to soil N due to fertilizer runoff or deposition. These variable species responses also indicate that taking a phylogenetic perspective may provide the generality required to more efficiently predict the growth responses of species to global climate change, rather than assessing the responses of multiple species individually.
Phylogenetic Similarity in an Ecosystem Response
This study is among the first, to our knowledge, to examine the growth responses of any taxa to global change within a phylogenetic context (but see , – for non-growth responses). We found a strong, shared response in plant performance within GL2. Species responses to elevated CO2 and soil N were determined by genetic lineage, where phylogenetically shared traits resulted in similar species responses within this group. Similar to the species level analysis, a number of genetic lineages did not appear to respond to elevated CO2 and N, whereas due to phylogenetically shared traits (e.g. larger root:shoot), GL2 responded strongly to elevated CO2 and soil N fertilization.
Genetic lineage level analyses showed that GL2 responded significantly differently to elevated CO2 and N than other genetic lineages; these species demonstrated strong, similar responses to elevated CO2 and N. The root:shoot differed significantly among genetic lineages, where GL2 displayed, on average, a 50% greater root:shoot. This shared trait possibly allowed for greater nutrient utilisation, thus stimulating the CO2 fertilisation effect . As these species in GL2 generally inhabit nutrient poor areas , this trait may have evolved in response to these environmental conditions.
The shared responses of GL2 to elevated CO2 and N, suggests that the growth responses of species to the global change factors of elevated CO2 and N may be phylogenetically biased, and a result of shared phylogenetic traits among closely related species , . Therefore, under a competitive environment containing a number of phylogenetic lineages, there may be ‘winner’ lineages and ‘loser’ lineages. For example, under elevated atmospheric CO2 concentrations and high soil N availability, in communities containing species from both GL1 and GL2, species from GL2 may outperform those from GL1. Willis et al.  applied similar phylogenetic methods to explain the invasiveness of non-native plant groups in Concord, MA (USA), where non-native groups displayed a greater ability to adjust flowering time in response to climate change; flowering time was postulated to be linked to fitness through ecological mismatches such as pollination. The results of our study indicate that a phylogenetic approach may provide a mechanism whereby highly idiosyncratic species-specific responses to elevated CO2 and N fertilization may be generalised across closely related species, perhaps providing a better understanding of the effects of global change on ecosystem dynamics.
When species responses were analysed for the effects of subgenus, the subgenera differed in belowground biomass and root:shoot but did not significantly interact with CO2 or N. These results indicate that there is a phylogenetic effect and the two subgenera respond to CO2 and N fertilization in the same way; thus this level of phylogeny may be too broad to differentiate effects of CO2 and N on morphological traits. Overall, these results suggest that more recently evolved traits at the lower phylogenetic grouping (genetic lineage) level are likely to be responsible for the large proportion of variation among species in responses to elevated CO2 and N fertilization.
Conclusions and Implications
Predictions of future plant distributions and the sustainability of the services those ecosystems provide are often made using niche-based models that implement correlative methods that relate the presence or absence of species across environmental gradients , . These models may be species-specific or generalised over species with similar fundamental niches. Using species-specific responses to both elevated CO2 and N may not be the ideal tool to use for predicting the responses of ecosystems to global environmental change , for conservation or climate change mitigation. The results of this study suggest that the responses of forest trees to elevated CO2 and N may not be random, but phylogenetically biased. A phylogenetic approach may provide a possible alternative to species-specific studies since it could be applied across many plant groups and ecological contexts. To consolidate our findings, further studies are warranted. For example, field studies would determine if phylogenetic patterns in species responses persist while subject to natural conditions. Understanding the evolutionary relationships of species may help us better understand and predict the future ecology of forest communities and as phylogenetic information continues to become exponentially more available, its utilization could complement the plant functional group approaches that are currently represented in many dynamic global vegetation models , . However, this raises the question of which phylogenetic level should be chosen to best predict species responses? The utilisation of the phylogenetic level which most adequately represents the distribution of the climatically relevant traits would be most pertinent.
Thanks to the support staff at the Central Science Laboratory at UTAS for use of molecular laboratory facilities. Thanks to Brad Potts, René Vaillancourt and Mark Hovenden for helpful discussions. Thanks also to Mark Hovenden for his expertise in climate change experiments and design as well as Tracy Winterbottom for expert assistance in the greenhouse. Finally, thanks to Camilla Bloomfield and Danny Lusk for their assistance in the glasshouse and lab.
Conceived and designed the experiments: JKB JAS AL SKC JO. Performed the experiments: JKS. Analyzed the data: JKS. Wrote the paper: JKS JAS JO SKC DS AL JKB.
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