Knowing the national disease burden of severe influenza in low-income countries can inform policy decisions around influenza treatment and prevention. We present a novel methodology using locally generated data for estimating this burden.
Methods and Findings
This method begins with calculating the hospitalized severe acute respiratory illness (SARI) incidence for children <5 years old and persons ≥5 years old from population-based surveillance in one province. This base rate of SARI is then adjusted for each province based on the prevalence of risk factors and healthcare-seeking behavior. The percentage of SARI with influenza virus detected is determined from provincial-level sentinel surveillance and applied to the adjusted provincial rates of hospitalized SARI. Healthcare-seeking data from healthcare utilization surveys is used to estimate non-hospitalized influenza-associated SARI. Rates of hospitalized and non-hospitalized influenza-associated SARI are applied to census data to calculate the national number of cases. The method was field-tested in Kenya, and validated in Guatemala, using data from August 2009–July 2011. In Kenya (2009 population 38.6 million persons), the annual number of hospitalized influenza-associated SARI cases ranged from 17,129–27,659 for children <5 years old (2.9–4.7 per 1,000 persons) and 6,882–7,836 for persons ≥5 years old (0.21–0.24 per 1,000 persons), depending on year and base rate used. In Guatemala (2011 population 14.7 million persons), the annual number of hospitalized cases of influenza-associated pneumonia ranged from 1,065–2,259 (0.5–1.0 per 1,000 persons) among children <5 years old and 779–2,252 cases (0.1–0.2 per 1,000 persons) for persons ≥5 years old, depending on year and base rate used. In both countries, the number of non-hospitalized influenza-associated cases was several-fold higher than the hospitalized cases.
Citation: Fuller JA, Summers A, Katz MA, Lindblade KA, Njuguna H, Arvelo W, et al. (2013) Estimation of the National Disease Burden of Influenza-Associated Severe Acute Respiratory Illness in Kenya and Guatemala: A Novel Methodology. PLoS ONE 8(2): e56882. https://doi.org/10.1371/journal.pone.0056882
Editor: Benjamin J. Cowling, University of Hong Kong, Hong Kong
Received: November 2, 2012; Accepted: January 15, 2013; Published: February 27, 2013
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: Work in Guatemala was supported by Cooperative Agreement UO1 GH000028-02 from the Centers for Disease Control and Prevention (http://www.cdc.gov/). Work in Kenya was supported by the Centers for Disease Control and Prevention and by the Wellcome Trust (http://www.wellcome.ac.uk/) , . These funders had no role in they study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Influenza disease burden data are sparse in low and lower-middle income countries ,,,,,. Local data on influenza disease incidence and case counts are useful for decision makers in these countries to assess the public health importance of influenza, to identify high risk groups and regions, to allocate resources efficiently, and to consider the cost-effectiveness of preventive strategies, such as vaccination.
Determining the amount of influenza-associated severe disease will likely have greater public health significance in low and lower-middle income countries where mortality from infectious diseases is still high. Estimates of influenza-associated severe acute respiratory illness (SARI) incidence have been calculated in some low and lower-middle income countries from population-based surveillance ,,,,,,. These estimates are usually limited to the area where the surveillance is performed. However, in many countries there is regional variability in risk factors, epidemiology, and healthcare-seeking practices that can affect hospitalized influenza-associated SARI rates and make it challenging to extrapolate rates from a small area to the whole country. The more country-specific data that are utilized to estimate the total number of influenza cases, the more credible this estimate is likely to be to public health decision makers within the country.
We present here a novel, replicable methodology using locally-generated data for estimating the national number of cases of influenza-associated SARI in low and lower-middle income countries and provide results of the initial field test in Kenya. The method was also validated using different case-definitions for influenza-associated pneumonia from Guatemala.
Siaya District Hospital (SDH) is located in Siaya District, Nyanza Province in rural western Kenya. HIV prevalence in Nyanza Province was 13.9% among adults in 2009 . Population-based surveillance at SDH is embedded in a larger Health and Demographic Surveillance System (HDSS) . For this analysis, a case of SARI in children <5 years was defined as a modification of the WHO definition of pneumonia as any child, residing in a defined catchment area, hospitalized with cough or difficulty breathing and any one of the following: tachypnea for age group, unable to drink or breastfeed, vomits everything, convulsions, lethargic or unconscious, nasal flaring, grunting, oxygen saturation <90%, chest indrawing, or stridor in a calm child ,. SARI for persons ≥5 years was defined as any hospitalized case with cough, difficulty breathing, or chest pain during the previous 14 days. The denominator used to calculate rates was from Karemo Division, the division located closest to SDH. No other inpatient facilities exist in Karemo.
Kilifi District Hospital (KDH) is located in Kilifi district, Coast province in eastern Kenya. In 2009 among adults, Coast province had an HIV prevalence of 4.2% . A HDSS is in operation within the district run by the KEMRI-Wellcome Trust Research Programme, which allows accurate determination of age-specific denominators ,[ 16]. For most residents of this study area, KDH is the nearest inpatient facility; however, there are three other inpatient hospitals within the district. KDH only had population-based data for children <5 years for August 2009–July 2010. The same case definition of SARI in children was used as in Siaya. The base rates calculated for Kilifi were calculated using administrative sub-locations within 5 km of KDH.
Sentinel hospital influenza surveillance in Kenya was established in 2007 and took place in all 8 provinces. Specimens were collected from hospitalized patients who met the SARI case definition, which for persons ≥5 years old was defined as a fever of ≥38°C with cough or shortness of breath or difficulty breathing and for children was defined as given above . Influenza vaccine is rarely used in the public or private sector in Kenya, as in other sub-Saharan African countries .
Overview of burden calculation
Figure 1 provides an overview of the methodology.
Data input steps are in white boxes and data output are in shaded boxes.
Step 1. Calculate incidence rate of hospitalized SARI from base province (Appendix S1, Equation 1).
From population-based surveillance sites with known catchment populations, we calculated the rate of hospitalized SARI, referred to as the ‘base rate.’ We used a catchment area of 5 kilometers from the district hospital as our denominator, because we felt persons within this area would be most likely to seek care at this facility and we could define the most accurate incidence, as healthcare utilization has been shown to decrease with greater distance from a health facility in Africa . The number of SARI patients admitted to the surveillance hospital for each year of surveillance was divided by the age-specific hospital catchment populations.
Step 2a. Calculate an adjustment factor for SARI risk factors for each province (Appendix S1, Equation 2a).
We calculated an adjustment factor for each province in the country based on known risk factors for SARI adapted from a method used to calculate the global incidence of pneumonia in children by Rudan et al., which applied adjustments for five risk factors for pneumonia in low and lower-middle income countries, including malnutrition (weight-for-age z-score <−2), low birth weight (≤2500 g), non-exclusive breastfeeding (during the first 4 months of life), household air pollution (defined as using solid fuels), and crowding (defined as ≥5 people per household) . Our adjustments were made based on the prevalence of these risk factors in each province and their relative risk for childhood pneumonia as defined by Rudan et al. based on their review of the literature (Table 1) ,[ 20]. Because of the elevated risk for hospitalized SARI in HIV-infected persons, we added an adjustment for HIV prevalence using the same equation ,,,,,. We did not have data on HIV prevalence in children in Kenya, therefore we calculated this prevalence using an algorithm which took into account the prevalence of HIV-infected mothers from antenatal clinics and the expected vertical transmission rate, accounting for penetration of prevention-of-mother-to-child-transmission programs, and transmission through breastfeeding (Appendix S2). For persons ≥5 years old, the risk factors used for adjustment were household air pollution, crowding, and HIV prevalence. Due to a lack of studies found during a literature review of these risk factors for adult pneumonia, we assumed that the relative risk of SARI for each risk factor was the same as for children.
Step 2b. Apply adjustments for risk factors and for healthcare-seeking for SARI by province (Appendix S1, Equation 2b).
The rate of hospitalized SARI by province was calculated by applying the risk-factor adjustment described in Step 2a and further adjusting for healthcare-seeking practices using a ratio of healthcare-seeking in the base province to each other province. Since 2007, national Demographic and Health Surveys (DHS) have included a standardized question that asks about healthcare-seeking practices for acute respiratory illness (ARI) . The question currently asks caretakers ‘if their children under age five had been ill in the two weeks preceding the survey with a cough accompanied by short, rapid breathing or difficulty breathing which the mother considered to be chest-related.’ If yes, the caretaker is asked if he or she ‘sought advice or treatment from a health facility or a provider’, which excludes pharmacies, shops and traditional healers. The percentage of children with ARI in the past two weeks who sought care at a health facility or provider is reported by administrative region in the country (e.g., province). As there was no question in the DHS asking about healthcare-seeking for adults and since the relative rather than absolute healthcare-seeking by province was more important for the adjustment, we used the same proportion for adults who sought care as for children from the DHS. We also assumed that healthcare-seeking for SARI in a province would be proportional to healthcare-seeking for ARI as defined in the DHS, and applied the ratio of these percentages to further adjust the rate of SARI for each province compared to the base rate province.
Step 3. Apply the percentage of SARI associated with influenza by province.
(Appendix S1, Equation 3.) The percentage of hospitalized SARI associated with influenza A and B viruses was obtained from established sentinel influenza surveillance sites. In Kenya, each province had one sentinel surveillance site and in Guatemala only the two provinces with population-based SARI surveillance had influenza-specific data available for this analysis. In Kenya, for provinces with <25 cases of SARI during a surveillance year, we considered the estimate as unstable due to small numbers or insensitive surveillance, and so used a weighted average of the percentage of hospitalized influenza-associated SARI from provinces with ≥25 cases, weighted by the number of samples taken. We applied these percentages to the rate of hospitalized SARI by province to determine the rate of hospitalized influenza-associated SARI for each province.
Step 4. Calculate and apply an adjustment for the rate of non-hospitalized SARI.
(Appendix S1, Equation 4.) Using data from published healthcare utilization surveys asking about healthcare-seeking practices for pneumonia, we estimated the rate of non-hospitalized SARI ,,. In these surveys, ‘pneumonia’ was defined as cough or difficulty breathing for more than two days or a diagnosis of ‘pneumonia’ by a healthcare worker. This estimation was based on the assumption that those patients have the same severity of illness, but did not seek care due to lack of access. We felt that compared to the DHS, the healthcare utilization survey healthcare-seeking questions were more relevant for SARI, as the questions focused on pneumonia, rather than the more nonspecific ARI question of the DHS; we expected more healthcare-seeking for more severe episodes like SARI than for all ARI. In addition, the healthcare utilization survey questions were asked for both children and adults. In both countries the healthcare utilization survey defined pneumonia as, ‘cough or difficulty breathing that lasted more than 2 days or a diagnosis of pneumonia given by a doctor or professional healthcare provider in the last year’. Respondents who reported a pneumonia episode in the past year were asked about healthcare-seeking. For this analysis, we considered the percentage of patients with pneumonia who sought care at a hospital as indicative of the percentage of persons who would have access to a hospital if they were to have an episode of influenza-associated SARI. Because healthcare utilization survey data was only available in one province in Kenya and two departments in Guatemala, we adjusted the healthcare utilization survey-derived percentage of those who sought care at a hospital for pneumonia to each province by applying the same ratio of healthcare-seeking for ARI from the DHS that we used in Step 2b; although in this case the ratio was for each province divided by the province in which the healthcare utilization survey was done (which may or may not have been the same as the province where the base rate of SARI was obtained).
Step 5. Multiply rates by the provincial populations (Appendix S1, Equation 5.).
The age-specific provincial populations were multiplied by the rates of hospitalized and non-hospitalized influenza-associated SARI to determine the number of cases for each province. The populations of the provinces were obtained from the most recent national census data, with projected annual population growth ,. The numbers of cases in each province were summed to calculate the national number of cases.
Confidence intervals were estimated by bootstrapping each data input in steps 1–4 above 1,000 times which resulted in 1,000 estimates of the number of hospitalized and non-hospitalized influenza-associated cases in the country. The upper and lower limits of the 95% confidence intervals were the 2.5th and 97.5th percentiles of these estimates, respectively. Confidence intervals are not symmetric because some inputs were re-sampled on the margins of the parameter space (e.g. proportions close to 0 or 1), reducing their potential variability in only one direction.
We validated this methodology in Guatemala using the slightly different case definitions used in surveillance there. The Centers for Disease Control and Prevention (CDC), in conjunction with the University of the Valley of Guatemala (UVG) and the Guatemalan Ministry of Public Health and Social Assistance, conducted population-based surveillance for pneumonia in two departments --in Santa Rosa, a rural lowland department in southeast Guatemala, at the National Hospital of Cuilapa, and in Quetzaltenango, a semi-urban highland department in western Guatemala, at Western National Hospital. During the 2009 influenza pandemic year, immunization for pH1N1 was reasonably high, but coverage for seasonal strains is typically low . At both Guatemalan sites, a case of pneumonia was defined as a hospitalized patient, residing in the hospital's pre-defined catchment area with at least one sign of an acute infection (e.g. fever, abnormal white blood cell count, hypothermia) and at least one sign or symptom of a respiratory tract illness (e.g. cough, rapid breathing, production of phlegm, chest pain, difficulty breathing) . All patients meeting the pneumonia case definition were tested for influenza virus using real-time PCR . Besides Quetzaltenango and Santa Rosa, no other sites had complete influenza sentinel surveillance data available. In Guatemala, there were a few deviations from the methodology described above. In Step 1, the base rate included an adjustment for the proportion of SARI cases hospitalized at non-surveillance hospitals within the catchment area. Also, there was no recent DHS data for Guatemala, but a similar survey (National Survey of Maternal and Child Health [ENSMI]) contained data on healthcare-seeking practices (Step 2b) and risk factor prevalence (Step 2a) . Finally, because prevalence of HIV remains low (<1%), no adjustments were made for HIV in Step 2a.
In the first year, the base rates of hospitalized SARI for children <5 years old in Siaya and Kilifi were 55.6 and 27.3 per 1,000 persons, respectively, and for persons ≥5 years old were 3.1 per 1,000 persons in Siaya (Table 2 and Table 3). In the second year, rates were slightly lower in children and slightly higher in persons ≥5 years. Risk factors for SARI and healthcare-seeking practices varied by province. This led to variability in the rates of hospitalized SARI by province. For example, during the first year (Karemo base rate), the point estimates of hospitalized SARI rates by province ranged from 16.6–55.6 per 1,000 persons for children and 1.4–3.1 per 1,000 for persons ≥5 years old.
The percentage of SARI with influenza virus detected varied by year and province (Figure 2). In Kenyan children, this percentage ranged from 3.6% to 16.0%. In Kenyan persons ≥5 years, the percentage ranged from 9.6% to 14.3%. The rates of influenza-associated hospitalized SARI were higher among children <5 years old than among persons ≥5 years old in both sites. Rates varied by province (Table 2 and Table 3). Forty-eight percent of children and 34% of older persons who reported pneumonia sought care at any hospital . After adjustment, the rate of non-hospitalized influenza-associated SARI was higher than hospitalized influenza-associated SARI for persons ≥5 years old in Kenya in all provinces (Table 3), and for children in Kenya in most provinces (Table 2).
August 2009-July 2011. Panel A-Children <5 years old. Panel B- Persons ≥5 years old.
In Kenya (2009 population of 38.6 million persons), the point estimates for the annual number of hospitalized influenza-associated SARI cases ranged from 17,129–27,659 for children <5 years old (2.9–4.7 per 1,000 persons) and 6,882–7,836 for persons ≥5 years old (0.21–0.24 per 1,000 persons), depending on year and base rate used (Table 4). The point estimates for the annual number of non-hospitalized influenza-associated SARI cases ranged from 19,798–30,275 (3.3–5.1 per 1,000 persons) for children <5 years old and 13,592–15,270 for persons ≥5 years old (0.42–0.47 per 1,000 persons), depending on year and base rate used (Table 4). The uncertainty ranges around the annual burden estimates are shown in Table 4.
In Guatemala in the first year, the base rates of hospitalized pneumonia for children <5 years old in Quetzaltenango and Santa Rosa were 9.7 and 8.7 per 1,000 persons, respectively, and for persons ≥5 years old were 0.67 and 0.86 per 1,000 persons, respectively (Table S1, Table S2, Table S3, Table S4). In the second year, rates were slightly higher for children and similar for older persons. Risk factors and healthcare-seeking practices varied by province. This led to variability in rates of hospitalized pneumonia by department. For example, using the Santa Rosa base rate in the first year, hospitalized pneumonia rates by department ranged from 8.8–15.7 per 1,000 persons for children <5 years old and 0.7–1.5 per 1,000 for persons ≥5 years old.
The percentage of pneumonia cases with influenza virus detected varied by year and department (Figure 2). In Guatemalan children, this percentage ranged from 5.5% to 8.6%. In persons ≥5 years, this percentage ranged from 6.6% to 19.9%. In the Santa Rosa area, 28% of children and 11% of older persons who reported pneumonia sought care at any hospital . At the Quetzaltenango site, 40% of children and 16% of older persons who reported pneumonia sought care at any hospital (Oliver Morgan, personal communication).
In Guatemala (2011 population of 14.7 million persons), the point estimates for the annual number of hospitalized cases of influenza-associated pneumonia ranged from 1,065–2,259 (0.5–1.0 per 1,000 persons) among children <5 years old and 779–2,252 cases (0.1–0.2 per 1,000 persons) for persons ≥ 5 years old, depending on year and base rate used. The point estimates for the annual number of non-hospitalized cases of influenza-associated pneumonia ranged from 2,033–4,312 (0.9–2.0 per 1,000 persons) among children <5 years old and 4,592–13,268 cases for persons ≥5 years old (0.4–1.1 per 1,000 persons), depending on year and base rate used.
Our method for calculating the national number of influenza-associated SARI has several advantages. First, it incorporates as much data from within the country as available, adjusting for regional differences in SARI risk factors and influenza prevalence. In order to make informed policy decisions, countries likely perceive data generated within their own country as more relevant and persuasive . For countries without population-based surveillance, however, base rates of SARI can be used from epidemiologically-similar neighboring countries. Second, our approach emphasizes the use of sentinel laboratory-based surveillance data and reinforces the need to assure high-quality influenza surveillance . Third, the estimate incorporates both hospitalized and non-hospitalized cases. Estimates of hospitalized cases alone can be utilized for planning care and treatment costs. However, in most low and lower-middle income countries, healthcare-seeking practices at hospitals can be low, particularly in rural areas where many persons die at home ,,. The full burden of severe influenza disease in low and lower-middle income countries, and the potential impact of interventions like vaccination, requires quantification of non-hospitalized cases as well.
For children <5 years of age, we found incidences of influenza-associated SARI similar in magnitude to those presented for low and lower-middle income countries from the same region in a recent systematic review of global influenza burden in children 3]. For persons ≥5 years old, the rates we found for influenza-associated SARI are similar to estimates from Bangladesh, Thailand, and another part of western Kenya ,,. Of note, our estimates included the period when pandemic H1N1 appeared in both countries, which might have led to changes in healthcare-seeking practices and influenza incidence that were not representative of normal years ,. Kenya was unique in having two different sites performing laboratory-based, population-based surveillance for hospitalized influenza in children, which allowed us to further check the accuracy of our methodology ,. When we used surveillance in Kilifi in Coast Province for the base rate in our method, we estimated a rate of hospitalized influenza-associated SARI of 2.3 per 1,000 (95% CI: 1.6–3.4) in Nyanza Province (Table S5), which is similar, with overlapping confidence intervals, to that found from a published report in Nyanza Province (1.4 per 1,000, 95% CI 1.2–1.7); the differences could be due to slightly different case definitions, catchment designs, and years of inclusion .
Alternate methods have been used to estimate national influenza case counts. One methodology is to use administrative data, such as national hospital discharge summaries based on ICD coding or vital statistics registries, to ascertain the number of hospitalizations and deaths associated with respiratory diseases. Such models have been used in the U.S., Canada, Hong Kong and South Africa ,,,,,. However, reliable longitudinal administrative data is not available in most low and lower-middle income countries. A second methodology is to perform healthcare utilization surveys at each sentinel surveillance site to define the incidence of both hospitalized and non-hospitalized SARI, since healthcare-seeking practices can differ regionally. This approach has been used in Bangladesh and Thailand ,,. While this approach can succeed in a research-oriented, well-resourced setting, this is not readily applicable to most low and lower-middle income countries with recently established sentinel influenza surveillance. Properly done healthcare utilization surveys are both time- and resource-intensive ,,. A more economical and perhaps more accurate approach would be to use data from one well-executed healthcare utilization survey, and adjust the results for every province using readily available data (e.g. DHS surveys), as done in our method .
Our method has several potential limitations. First, the estimates are sensitive to the base rate of SARI used. For example, to calculate incidence fully it is important to capture all the cases within a defined catchment area, which might mean enrolling children from multiple hospitals that serve the catchment area. Also, because healthcare utilization is suboptimal in many low income countries, rates tend to be higher, and perhaps more accurate of the true incidence, when using catchment populations closer to the facilities where cases are captured ,,,. We chose to use catchment areas within a 5 kilometers radius from our base rate surveillance hospitals in Kenya. Second, in Kenya, different case definitions were used for SARI among adults in the population-based surveillance and sentinel influenza surveillance, the main difference being that the latter required documented fever . It has been shown that the adult SARI case definition commonly used for sentinel surveillance misses many cases of influenza-associated hospitalization, and we considered it important for a burden estimate to use a broader, more sensitive case definition for the base rate . Even so, we likely missed some non-respiratory influenza-associated hospitalizations in which influenza might have exacerbated underlying chronic illnesses . Third, we assumed that the severity of non-hospitalized SARI was equivalent to hospitalized SARI, which might not have been the case. In addition, because countries may use different case definitions for SARI, care should be taken when comparing incidence estimated by this tool between countries. For example, we used Guatemalan data to validate our method, despite their use of a pneumonia case definition that differed from the SARI case definitions used in Kenya. While this prevents direct comparison of influenza case counts and incidence between Guatemala and Kenya, or other countries with SARI surveillance, the data are internally consistent for each country and demonstrate flexibility of the methodology in being able to use locally available surveillance definitions. Lastly, our methodology did not produce estimates of influenza-associated mortality, which are useful to decision makers.
Country-level influenza disease estimates will become increasingly important to Ministries of Health over the coming years. As the role of influenza as a major public health concern comes into sharper focus, countries will need representative and ideally country-specific data to make decisions about treatment and preventive strategies, including targeted vaccination with currently available influenza vaccines. Knowing the number of severe influenza cases in their countries, as well as the associated costs of illness, will afford decision makers the type of data needed to formulate sound national policies.
Rates (per 1,000) of hospitalized and non-hospitalized influenza-associated pneumonia in Guatemala among children <5 years of age, August 2009 to July 2011. Santa Rosa (bolded) surveillance was used for the base rate as well as the healthcare utilization survey.
Rates (per 1,000) of hospitalized and non-hospitalized influenza-associated pneumonia in Guatemala among persons ≥5 years of age, August 2009 to July 2011. Santa Rosa (bolded) surveillance was used for the base rate as well as the healthcare utilization survey.
Rates (per 1,000) of hospitalized and non-hospitalized influenza-associated pneumonia in Guatemala among children <5 years of age, August 2009 to July 2011. Quetzaltenango (bolded) surveillance was used for the base rate as well as the healthcare utilization survey.
Rates (per 1,000) of hospitalized and non-hospitalized influenza-associated pneumonia in Guatemala among persons ≥5 years of age, August 2009 to July 2011. Quetzaltenango (bolded) surveillance was used for the base rate as well as the healthcare utilization survey.
Rates and 95% confidence limits (per 1,000) of hospitalized and non-hospitalized influenza-associated severe acute respiratory illness (SARI) in Kenyan children <5 years of age, August 2009 to July 2010. Kilifi, Coast province (bolded) surveillance was used for the base rate.
Equations used in calculation of annual number of cases of influenza-associated severe acute respiratory infection (SARI) cases.
Disclaimer: the findings and conclusions are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention. This paper was published with the permission of the director of the Kenya Medical Research Institute.
Conceived and designed the experiments: JAF AS DRF. Performed the experiments: JAF AS MAK KAL HN WA SK GE NLP JM DJN MN SK JAM SJO MAW DRF. Analyzed the data: JAF AS. Wrote the paper: JAF AS DRF.
- 1. Feikin DR, Ope MO, Aura B, Fuller JA, Gikunju S, et al. (2012) The population-based burden of influenza-associated hospitalization in rural western Kenya, 2007–2009. Bull World Health Organ 90: 256–263A.
- 2. Homaira N, Luby SP, Petri WA, Vainionpaa R, Rahman M, et al. (2012) Incidence of respiratory virus-associated pneumonia in urban poor young children of Dhaka, Bangladesh, 2009–2011. PLoS One 7: e32056.
- 3. Nair H, Brooks WA, Katz M, Roca A, Berkley JA, et al. (2011) Global burden of respiratory infections due to seasonal influenza in young children: a systematic review and meta-analysis. Lancet 378: 1917–1930.
- 4. Razuri H, Romero C, Tinoco Y, Guezala MC, Ortiz E, et al. (2012) Population-based active surveillance cohort studies for influenza: lessons from Peru. Bull World Health Organ 90: 318–320.
- 5. Yazdanbakhsh M, Kremsner PG (2009) Influenza in Africa. PLoS Med 6: e1000182.
- 6. Hammitt LL, Kazungu S, Morpeth SC, Gibson DG, Mvera B, et al. (2012) A preliminary study of pneumonia etiology among hospitalized children in Kenya. Clin Infect Dis 54 Suppl 2S190–199.
- 7. Azziz-Baumgartner E, Alamgir AS, Rahman M, Homaira N, Sohel BM, et al. (2012) Incidence of influenza-like illness and severe acute respiratory infection during three influenza seasons in Bangladesh, 2008–2010. Bull World Health Organ 90: 12–19.
- 8. Cohen SA, Klassen AC, Ahmed S, Agree EM, Louis TA, et al. (2010) Trends for influenza and pneumonia hospitalization in the older population: age, period, and cohort effects. Epidemiol Infect 138: 1135–1145.
- 9. Dawood FS, Fry AM, Muangchana C, Sanasuttipun W, Baggett HC, et al. (2011) A method for estimating vaccine-preventable pediatric influenza pneumonia hospitalizations in developing countries: Thailand as a case study. Vaccine 29: 4416–4421.
- 10. Simmerman JM, Chittaganpitch M, Levy J, Chantra S, Maloney S, et al. (2009) Incidence, seasonality and mortality associated with influenza pneumonia in Thailand: 2005–2008. PLoS One 4: e7776.
- 11. Measure DHS (2010) Measure Demographic and Health Surveys. http://measuredhs.com
- 12. Adazu K, Lindblade KA, Rosen DH, Odhiambo F, Ofware P, et al. (2005) Health and demographic surveillance in rural western Kenya: a platform for evaluating interventions to reduce morbidity and mortality from infectious diseases. Am J Trop Med Hyg 73: 1151–1158.
- 13. Ortiz JR, Sotomayor V, Uez OC, Oliva O, Bettels D, et al. (2009) Strategy to enhance influenza surveillance worldwide. Emerg Infect Dis 15: 1271–1278.
- 14. WHO (2008) Integrated Management of Childhood Illness. World Health Organization. http://www.who.int/maternal_child_adolescent/documents/IMCI_chartbooklet/en/index.html
- 15. Nokes DJ, Ngama M, Bett A, Abwao J, Munywoki P, et al. (2009) Incidence and severity of respiratory syncytial virus pneumonia in rural Kenyan children identified through hospital surveillance. Clin Infect Dis 49: 1341–1349.
- 16. Scott JA, Bauni E, Moisi JC, Ojal J, Gatakaa H, et al. (2012) Profile: The Kilifi Health and Demographic Surveillance System (KHDSS). Int J Epidemiol 41: 650–657.
- 17. Gessner BD, Shindo N, Briand S (2011) Seasonal influenza epidemiology in sub-Saharan Africa: a systematic review. Lancet Infect Dis 11: 223–235.
- 18. Feikin DR, Nguyen LM, Adazu K, Ombok M, Audi A, et al. (2009) The impact of distance of residence from a peripheral health facility on pediatric health utilisation in rural western Kenya. Trop Med Int Health 14: 54–61.
- 19. Rudan I, Boschi-Pinto C, Biloglav Z, Mulholland K, Campbell H (2008) Epidemiology and etiology of childhood pneumonia. Bull World Health Organ 86: 408–416.
- 20. UNICEF (2011) Multiple Indicators Cluster Survey (MICS). New York: UNICEF. http://www.childinfo.org/mics3.html
- 21. Madhi SA, Ramasamy N, Bessellar TG, Saloojee H, Klugman KP (2002) Lower respiratory tract infections associated with influenza A and B viruses in an area with a high prevalence of pediatric human immunodeficiency type 1 infection. Pediatr Infect Dis J 21: 291–297.
- 22. Ope MO, Katz MA, Aura B, Gikunju S, Njenga MK, et al. (2011) Risk factors for hospitalized seasonal influenza in rural western Kenya. PLoS One 6: e20111.
- 23. Rates of mother-to-child transmission of HIV-1 in Africa, America, and Europe: results from 13 perinatal studies. The Working Group on Mother-To-Child Transmission of HIV. J Acquir Immune Defic Syndr Hum Retrovirol 8: 506–510.
- 24. Coutsoudis A, Dabis F, Fawzi W, Gaillard P, Haverkamp G, et al. (2004) Late postnatal transmission of HIV-1 in breast-fed children: an individual patient data meta-analysis. J Infect Dis 189: 2154–2166.
- 25. Iwuji CC, Mayanja BN, Weiss HA, Atuhumuza E, Hughes P, et al. (2011) Morbidity in HIV-1-infected individuals before and after the introduction of antiretroviral therapy: a longitudinal study of a population-based cohort in Uganda. HIV Med 12: 553–561.
- 26. Roca A, Sigauque B, Quinto L, Morais L, Berenguera A, et al. (2010) Estimating the vaccine-preventable burden of hospitalized pneumonia among young Mozambican children. Vaccine 28: 4851–4857.
- 27. Burton DC, Flannery B, Onyango B, Larson C, Alaii J, et al. (2011) Healthcare-seeking behaviour for common infectious disease-related illnesses in rural Kenya: a community-based house-to-house survey. J Health Popul Nutr 29: 61–70.
- 28. Lindblade KA, Johnson AJ, Arvelo W, Zhang X, Jordan HT, et al. (2011) Low usage of government healthcare facilities for acute respiratory infections in guatemala: implications for influenza surveillance. BMC Public Health 11: 885.
- 29. Deutscher M, Van Beneden C, Burton D (2012) Putting Surveillance Data into Context: The Role of Health Care Utilization Surveys in Understanding Population Burden of Pneumonia in Developing Countries. J Epidemiology and Public Health in press.
- 30. Central Bureau of Statistics [Kenya] (2010) 2009 Kenya Housing and Population Census: Volume 1A Population Distribution by Administrative Units. 1A..
- 31. Instituto Nacional de Estadisticas [Guatemala] (2012) 2002 Population Census (projections for 2011). http://www.ine.gob.gt/np/
- 32. Ropero-Alvarez AM, Whittembury A, Kurtis HJ, dos Santos T, Danovaro-Holliday MC, et al. (2012) Pandemic influenza vaccination: lessons learned from Latin America and the Caribbean. Vaccine 30: 916–921.
- 33. Reyes L, Arvelo W, Estevez A, Gray J, Moir JC, et al. (2010) Population-based surveillance for 2009 pandemic influenza A (H1N1) virus in Guatemala, 2009. Influenza Other Respi Viruses 4: 129–140.
- 34. WHO collaborating Centre for Influenza at CDC (2009) CDC protocol of real-time RTPCR for influenza A (H1N1). http://www.who.int/csr/resources/publications/swineflu/CDCRealtimeRTPCR_SwineH1Assay-2009_20090430.pdf
- 35. Instituto Nacional de Estadisticas [Guatemala] (2008) Encuesta Nacional de Salud Materno Infantil (ENSMI). http://www.ine.gob.gt/np/ensmi/index.htm
- 36. Stansfield SK, Walsh J, Prata N, Evans T (2006) Information to Improve Decision Making for Health. In: Jamison DT, Breman JG, Measham AR, Alleyne G, Claeson M et al.., editors. Disease Control Priorities in Developing Countries. Washington DC: The International Bank for Reconstruction and Development/The World Bank Group.
- 37. Introduction and transmission of 2009 pandemic influenza A (H1N1) Virus--Kenya, June-July 2009. MMWR Morb Mortal Wkly Rep 58: 1143–1146.
- 38. Onyango CO, Njeru R, Kazungu S, Achilla R, Bulimo W, et al. (2012) Influenza surveillance among children with pneumonia admitted to a district hospital in coastal kenya, 2007–2010. J Infect Dis 206 Suppl 1S61–67.
- 39. Cohen C, Simonsen L, Kang JW, Miller M, McAnerney J, et al. (2010) Elevated influenza-related excess mortality in South african elderly individuals, 1998–2005. Clin Infect Dis 51: 1362–1369.
- 40. Kwong JC, Stukel TA, Lim J, McGeer AJ, Upshur RE, et al. (2008) The effect of universal influenza immunization on mortality and health care use. PLoS Med 5: e211.
- 41. Li CK, Choi BC, Wong TW (2006) Influenza-related deaths and hospitalizations in Hong Kong: a subtropical area. Public Health 120: 517–524.
- 42. Simonsen L, Clarke MJ, Williamson GD, Stroup DF, Arden NH, et al. (1997) The impact of influenza epidemics on mortality: introducing a severity index. Am J Public Health 87: 1944–1950.
- 43. Thompson WW, Shay DK, Weintraub E, Brammer L, Cox N, et al. (2003) Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 289: 179–186.
- 44. Moisi JC, Nokes DJ, Gatakaa H, Williams TN, Bauni E, et al. (2011) Sensitivity of hospital-based surveillance for severe disease: a geographic information system analysis of access to care in Kilifi district, Kenya. Bull World Health Organ 89: 102–111.
- 45. Weber MW, Milligan P, Sanneh M, Awemoyi A, Dakour R, et al. (2002) An epidemiological study of RSV infection in the Gambia. Bull World Health Organ 80: 562–568.
- 46. Murray EL, Khagayi S, Ope M, Bigogo G, Ochola R, et al. (2012) What are the most sensitive and specific sign and symptom combinations for influenza in patients hospitalized with acute respiratory illness? Results from western Kenya, January 2007–July 2010. Epidemiol Infect 1–11.
- 47. Mullooly JP, Bridges CB, Thompson WW, Chen J, Weintraub E, et al. (2007) Influenza- and RSV-associated hospitalizations among adults. Vaccine 25: 846–855.