We conducted a prospective study in a cohort of short-term travelers assessing the incidence rate of anti-circumsporozoite seroconversion, adherence to chemoprophylaxis, symptoms of malaria during travel, and malaria treatment abroad.
Adults were recruited from the travel clinic of the Public Health Service Amsterdam. They kept a structured daily travel diary and donated blood samples before and after travel. Blood samples were serologically tested for the presence of Plasmodium falciparum anti-circumsporozoite antibodies.
Overall, the incidence rate (IR) of anti-circumsporozoite seroconversion was 0.8 per 100 person-months. Of 945 travelers, 620 (66%) visited high-endemic areas and were advised about both chemoprophylaxis and preventive measures against mosquito bites. Most subjects (520/620 = 84%) took at least 75% of recommended prophylaxis during travel. Travel to Africa, use of mefloquine, travel duration of 14–29 days in endemic areas, and concurrent use of DEET (N,N-diethyl-meta-toluamide) were associated with good adherence practices. Four travelers without fever seroconverted, becoming anti-circumsporozoite antibody-positive. All four had been adherent to chemoprophylaxis; two visited Africa, one Suriname, one India. Ten subjects with fever were tested for malaria while abroad and of these, three received treatment. All three were adherent to chemoprophylaxis and tested negative for anti-circumsporozoite antibodies.
Travel to Africa, using mefloquine, travel duration of 14–29 days in endemic areas, and use of DEET were associated with good adherence to chemoprophylaxis. The combination of chemoprophylaxis and other preventive measures were sufficient to protect seroconverting travelers from clinical malaria. Travelers who were treated for malaria abroad did not seroconvert.
Citation: Belderok S-M, van den Hoek A, Roeffen W, Sauerwein R, Sonder GJB (2013) Adherence to Chemoprophylaxis and Plasmodium falciparum Anti-Circumsporozoite Seroconversion in a Prospective Cohort Study of Dutch Short-Term Travelers. PLoS ONE 8(2): e56863. https://doi.org/10.1371/journal.pone.0056863
Editor: David Joseph Diemert, The George Washington University Medical Center, United States of America
Received: October 22, 2012; Accepted: January 15, 2013; Published: February 25, 2013
Copyright: © 2013 Belderok et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was conducted within the Amsterdam Academic Collaborative Center on Public Health: a collaboration between the Public Health Service of Amsterdam & the Academic Medical Center of the University of Amsterdam, the Netherlands. The Amsterdam Academic Collaborative Center on Public Health is financially supported by the Netherlands Organization for Health Research and Development (ZonMw; grant number 7115 0001, http://www.zonmw.nl/nl/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Half the world’s population is at risk of malaria , . An estimated 216 million malaria cases, and 655,000 deaths, occurred in 2010, mostly among the local population in malaria-endemic regions . Malaria also is a threat to the approximately 80–90 million travelers who visit the 106 endemic countries annually.
Travelers can protect themselves against malaria by using antimalarial chemoprophylaxis and preventive measures against mosquito bites. Recommendations for these preventive measures are based on the anticipated infection rate and drug resistance in Plasmodium falciparum –, and differ by country.
Risk estimates for malaria infection among travelers from nonendemic countries are usually based on transmission rates in endemic populations and reports of infections in returned tourists. Neither estimate correlates well with the risk for travelers , .
Studies that estimate incidence based on these reports lack information on how many travelers were protected by chemoprophylaxis and how many were treated for malaria abroad. They are, however, valid to estimate trends. In the Netherlands, the incidence of imported falciparum malaria among travelers declined from 10.0/10,000 in the year 2000 to 3.4/10,000 travelers in 2007, whereas the proportion of travelers who did not use chemoprophylaxis rose from 47% to 52% . Since most of the malaria cases occur in travelers who fail to use – or adhere to – the appropriate chemoprophylaxis , –, adherence to chemoprophylaxis is associated with protection against malaria. Prospective studies among travelers are more valid to estimate risks . Most prospective studies are incomplete in their assessment of adherence to chemoprophylaxis combined with serological testing for P. falciparum infection –; in fact there is only one study, from 1991–1992, which examined both adherence to chemoprophylaxis in relation to serological testing.
In order to contribute to a more definitive assessment of risks for travelers, we conducted a comprehensive prospective study as to the incidence rate of Plasmodium falciparum anti-circumsporozoite seroconversion, adherence to chemoprophylaxis, symptoms of malaria during travel, and malaria treatment abroad.
Study protocol was approved by the Medical Ethics Committee of the Academic Medical Center Amsterdam (MEC 06/016). Participants were included only with informed and written consent.
A prospective study was performed among persons attending the travel clinic of the Public Health Service Amsterdam from October 2006 to October 2007. All persons 18 years and older were eligible if they were planning to travel for 1 to 13 weeks to one or more malaria-endemic countries. Countries were grouped in continents according to the composition of macro geographical (continental) regions described by the United Nations Statistics Division . All participants were seen by a doctor or nurse specialized in travel medicine. Based on Dutch national guidelines for travelers’ health advice , participants traveling to low-risk malaria-endemic areas (‘low-endemic areas’) were advised about strict preventive measures against mosquito bites without chemoprophylaxis; participants traveling to intermediate- and/or high-risk malaria-endemic areas (‘high-endemic areas’), were advised about strict preventive measures against mosquito bites and antimalarial chemoprophylaxis. Depending on travel destination and travelers’ characteristics, atovaquone-proguanil, mefloquine, doxycycline, or proguanil were recommended in the Netherlands as chemoprophylaxis against infection with P. falciparum malaria . Travelers taking mefloquine were advised to start 3 weeks prior to arrival in high-endemic areas, in case of atovaquone-proguanil one day. Travelers taking proguanil or doxycycline start on the day of arrival. Travelers were advised to continue mefloquine, proguanil, and doxycycline 4 weeks after leaving high-endemic areas, and atovaquone-proguanil for 7 days. Participants received a prescription for the appropriate antimalarial chemoprophylaxis, oral and written information about malaria, the use of chemoprophylaxis, and preventive measures against mosquito bites.
A standard questionnaire in Dutch or English was used before departure to collect data on sociodemographics, travel history, and purpose of travel (tourism, work or education, or visiting friends and/or relatives [VFR]). Participants were given a thermometer and were asked to take their temperature if they felt feverish. They were also asked to keep a structured travel diary, recording itinerary, use of antimalarial chemoprophylaxis, use of preventive measures against mosquito bites such as a mosquito net and/or an insect repellent containing N,N-diethyl-meta-toluamide (DEET) and/or sleeping in air conditioned rooms, signs of disease (fever), doctor visits, and (self)treatment. Participants made daily diary entries from the day they arrived at their destination to 2 weeks after their return, to encompass the incubation period of Plasmodium falciparum malaria and to register adherence to chemoprophylaxis after return. After travel, a nurse checked the diary in the presence of the participant. Participants were then asked if they had taken the advised chemoprophylaxis prior to travel – if applicable.
The number of days spent in malaria-endemic areas was defined as ‘exposure time’. Participants were considered ‘adherent’ to chemoprophylaxis if they took at least 75% of their recommended tablets as prescribed during their stay in high-endemic areas. Adherence before and after travel were separately described. The use of DEET, use of a mosquito net, and sleeping in an air-conditioned room were quantified in percentages by dividing the number of days the preventive measure was used by the number of days spent in a malaria-endemic area; and we dichotomized these variables by using the mean of this proportion in the total study population as the cut-off. Fever was self-reported (feeling feverish or thermometer confirmed fever with a temperature of 38°C or higher) starting 1 week after arriving in a malaria-endemic area.
Before departure and 2 to 6 weeks after return, participants donated venous blood samples for serology. All blood samples were immediately stored at 6°C. Blood samples for serologic testing were centrifuged (Hettich Rotixa 50 S, see APP/407: program 1, 10 min. 3000 rpm (210 g)) and frozen at −80°C within 24 hours until use. The CSP-ELISA (circumsporozoite protein enzyme-linked immunosorbent assay) was used to test for the presence of P. falciparum anti-circumsporozoite antibodies according to standard protocols . The optical density (OD) was read at 450 nm (reference filter 620 nm). Positive-control pooled plasma samples from Tanzanian individuals and negative-control plasma samples from Dutch individuals were included in each plate. The threshold for positivity for anti-circumsporozoite antibodies was calculated as the mean OD of the 7 negative control plasma samples plus 3 standard deviations. If the (OD) in serum samples after travel tested positive and showed a ≥2-fold increase compared to the OD value pretravel, these were defined as anti-circumsporozoite antibody seroconversion. This anti-circumsporozoite antibody seroconversion was considered indicative of exposure to P. falciparum during present travel.
Data analysis was performed with SPSS version 18.104.22.168 (2010, IBM, Somers, USA). Attack rates (ARs) and incidence rates (IRs) were based on positive anti-circumsporozoite antibody seroconversion indicative for exposure to P. falciparum. ARs were calculated by dividing the number of study subjects displaying seroconversion in the CSP-ELISA by the total number of participants at risk. IRs per 100 person-months were calculated by dividing the number of travelers with exposure to P. falciparum by our calculation of their exposure time. If a traveler was exposed to P. falciparum, we used half of their travel duration in endemic area as their exposure time; for travelers without exposure to P. falciparum, we used their total travel duration. Pearson chi-square tests of association were used to compare categorical variables between any two groups. Independent determinants for adherence to chemoprophylaxis and for use of DEET were identified by multiple logistic regression analysis and expressed as odds ratios with 95% confidence intervals. A p-value <0.05 was considered statistically significant.
A total of 945 travelers to malaria-endemic countries were recruited. Of these, 400 (42%) were male (Table 1). The majority (616, 65%) was under 45 years of age, most (783, 83%) had visited tropical or subtropical countries before, and 879 (93%) were born in a Western country; 815 (86%) traveled for holiday; 71 (8%) traveled for work or education, and 59 (6%) were VFR. More than half (529, 56%) stayed less than 14 days in malaria-endemic areas. The most frequently visited continent was Asia (454, 48%); 285 (30%) traveled to Africa and 206 (22%) to Latin America.
Of the 945 travelers, 620 (66%) were advised of both chemoprophylaxis and preventive measures against mosquito bites. The other 325 participants traveled to low-endemic areas.
Adherence to Chemoprophylaxis among Travelers to High-endemic Areas
Of the 620 travelers to high-endemic areas who were prescribed chemoprophylaxis, 449 were prescribed atovaquone-proguanil (48%), 91 proguanil (10%), 70 mefloquine (8%), and 10 (2%) other types of chemoprophylaxis, such as minocyclin, doxycycline, or a combination of chemoprophylaxis.
Of 620 travelers, 520 (84%) took at least 75% of the recommended tablets during their stay in high-endemic areas (Table 2). In multivariable analysis, travelers to Africa were significantly more adherent to chemoprophylaxis than travelers to Asia or Latin America (OR 3.5 (95% CI 1.9–6.5) and 2.7 (95% CI 1.4–5.3) respectively); travelers who spent 14–29 days in endemic areas were significantly more adherent compared to travelers who spent either ≤13 days or ≥29 days in endemic areas (OR 2.2 (95% CI 1.2–3.8) and 2.5 (95% CI 1.1–5.6) respectively); travelers who used DEET as an additional preventive measure in more than 50% of days spent in high-endemic areas were significantly more adherent to chemoprophylaxis compared to those who did not use DEET (OR 2.6 (95% CI 1.4–4.8)); and travelers using mefloquine were significantly more adherent compared to travelers using atovaquone-proguanil or proguanil (OR 5.3 (95% CI 1.2–23.1) and 6.0 (95% CI 1.3–27.5) respectively); Of 620 travelers, 466 (75%) took 100% of the recommended advised tablets during their stay in high-endemic areas, with the same determinants as for 75% adherence.
Table 3 shows the adherence to mefloquine, atovaquone-proguanil and proguanil prior to, during, and after stay in endemic area(s) among travelers who started with prophylaxis. The highest adherence percentage for all categories was found among travelers using mefloquine.
Antimosquito Preventive Measures
Of 945 travelers, 791 (84%) used DEET, 465 (49%) used a mosquito net, and 544 (58%) slept in an air-conditioned room at least once.
Multivariable analysis showed that travelers born in a non-Western country used significantly less DEET and also slept significantly less often under a mosquito net than travelers born in a Western country. Female travelers used significantly more DEET than male travelers. Older travelers used significantly less DEET or mosquito nets than younger travelers. Spending more days in endemic areas was an independent determinant for using less DEET and sleeping less often in an air-conditioned room. Travelers to high-endemic areas in general used a mosquito net significantly more often and slept less often in an air-conditioned room compared to travelers to low-endemic areas. Travelers to Africa or Latin America used a mosquito net significantly more often and slept less often in an air-conditioned room compared to travelers to Asia.
Of 945 travelers, only 938 serum samples were tested using the CSP-ELISA method because the sample was insufficient to test in 7 cases. Of the 938 samples, 4 (AR 0.4% (95% CI 0.1–1.0)) showed an anti-circumsporozoite antibody seroconversion. All 4 travelers were born in the Netherlands, had been 100% adherent to their chemoprophylaxis during travel, and none reported fever or had visited a doctor (Table 4). Of the 4 travelers, 2 stayed in a high-endemic area up to 14 days and 2 between 14 and 29 days; 2 visited Africa, 1 India and 1 Suriname. The overall IR of anti-circumsporozoite antibodies was 0.8 per 100 person-months (95% CI 0.3–2.0).
Of the 945 travelers, 74 (8%) reported fever after a median period of 17 days (IQR 12–26) in endemic areas. Of these 74, 67 (91%) used the provided thermometer and recorded fever with a median temperature of 38.7°C (IQR 38.2°C–39.6°C). Of the 74, 24 (32%) had visited low-endemic areas and 50 (68%) high-endemic areas. Of 24 travelers who reported fever in low-endemic areas, 13 (54%) sought medical attention abroad, 2 of whom were tested negative for malaria. Of 50 travelers to high-endemic areas, 17 (34%) consulted a doctor abroad, of whom 8 were tested for malaria and 3 were actually treated for malaria. These last 3 travelers had been fully adherent to chemoprophylaxis during travel and tested negative for anti-circumsporozoite antibodies.
In this prospective study with short-term travelers to malaria-endemic countries, self reported adherence to chemoprophylaxis was good. Best adherence was found among travelers to Africa, travelers using mefloquine as chemoprophylaxis, travelers who spent 14–29 days in endemic areas and travelers who were more adherent to use of DEET. Based on seroconversions for anti-circumsporozoite antibodies, we found an overall attack rate (AR) for P. falciparum of 0.4% and an overall incidence rate (IR) of 0.8 per 100 person-months.
Adherence to chemoprophylaxis found in our study is in agreement with self reported treatment adherence found in other studies, which ranged from 70%–89% for atovaquone-proguanil and 72%–95% for mefloquine , , , . Travelers to Africa were more adherent to chemoprophylaxis, which is also consistent with other studies , , . Even though in our travel consultation we do not communicate differences in risk between different high-endemic continents, it is possible that travelers know that malaria risk in Africa is higher than in other continents and therefore are more cautious. This may also be the reason that travelers to high-endemic areas used a mosquito net more often than in other areas. Further, we found good adherence to DEET to be independently related to good adherence to chemoprophylaxis, suggesting that those travelers who were more adherent to one preventive measure were also more likely to follow other preventive measures. These results are in agreement with previous reports , . The high level of adherence to mefloquine in our study suggests that this group of travelers experienced minimal side effects, which is supported by data in the daily diaries. One could argue that travelers who did not tolerate mefloquine during previous travel due to side effects chose to use alternative chemoprophylaxis, such as atovaquone-proguanil or doxycycline. However, previous travel experience was univariably not associated with better adherence to mefloquine, atovaquone-proguanil, or doxycycline.
Determinants of nonadherence to chemoprophylaxis found in previous studies were visiting friends and/or relatives (VFR), younger age, extensive travel experience, adventurous travel, and (assumed) adverse reactions , , , , . We did not find VFR to be less adherent than other groups of travelers. This is probably because Lobel et al and Ropers et al studied travelers flying back from destinations, whereas VFR in our study (only 7% of the total study population) were recruited in a pretravel clinic and are therefore not representative for VFR in general. The VFR in our study were probably more aware of the risk, therefore attended our pre-travel clinic and were also more likely to follow our recommended chemoprophylaxis. Indeed, imported malaria is mostly seen in VFR who did not seek pre-travel health advice and who never intended to use chemoprophylaxis, or who used chemoprophylaxis inadequately , , .
Furthermore, we did not find a relation between travel experience and adherence, but we did find less adherence to chemoprophylaxis in younger travelers, although not significantly.
Studies have shown that detecting anti-circumsporozoite antibodies can be used in nonimmune travelers using chemoprophylaxis as a measure of P. falciparum infection , , , , , . In our study population, 0.4% had a recent infection with P. falciparum sporozoites. All 4 travelers were infected in high-endemic areas, none reported fever or malaria treatment, and all had been adherent to chemoprophylaxis. This suggests that the use of chemoprophylaxis protected these 4 travelers from clinical malaria. There are a few other prospective studies on malaria infection using anti-circumsporozoite antibody tests in nonimmune travelers using chemoprophylaxis , , . The AR of 0.4% we found was lower than the AR found in 1991–1992 by Cobelens et al (1.3%), but their IR of 1.7 per 100 person-months is comparable to our IR (IR 0.8; 95% CI 0.3–2.0). Nothdurft et al published in 1999 an AR of 4.96% and Knappik et al found in 1999 an AR of 0.95%. Differences in ARs could be due to changes in incidences , , , but also to differences in group characteristics, countries visited, or the use of different ELISA tests. Therefore the studies cannot be compared.
Our study is one of the few comprehensive studies that combined P. falciparum anti-circumsporozoite seroconversion, adherence to chemoprophylaxis, and collection of clinical malaria data among travelers. The prospective nature of this study with blood samples pre- and post-travel allowed estimating the AR and IR of exposure to P. falciparum malaria. The daily diary entries, which minimized recall bias, provided a good record of the use of chemoprophylaxis and antimosquito preventive measures, fever, and treatment for malaria during travel.
Interpretation of our study results may be influenced by a number of shortcomings. First, the daily diary entries could have served as a reminder for travelers to take their chemoprophylaxis and therefore lead to better adherence to treatment. Second, because of the study design, the end date for travelers to fill in their diaries was 2 weeks after return, so we do not know if travelers using mefloquine or proguanil continued chemoprophylaxis after the 2 weeks. Treatment adherence during stay in endemic areas, however, remains most important in protection against infection with P. falciparum. Finally, measurement of malaria exposure by anti-CSP seroconversion has limitations due to the methodology. The sensitivity is limited due to small numbers of inoculated sporozoites by infected mosquitoes and their short life span . Development of a detectable anti-CSP response is dose related and requires multiple inoculations , . Preliminary data from controlled human malaria infections in malaria naïve Dutch volunteers  show that anti-CSP seroconversion occurs in 30% of the volunteers after a single infection with 5 infected mosquitoes (Sauerwein, unpublished). Therefore, the actual number of travelers exposed to sporozoites may be higher for all endemic areas, and our AR and IR likely represent underestimations. The 3 seronegative travelers treated for malaria may have been exposed and infected.
In conclusion, travel to Africa, using mefloquine, travel duration of 14–29 days in endemic areas, and good adherence to DEET were associated with good adherence to chemoprophylaxis. Adherence to chemoprophylaxis in combination with other preventive measures was good enough to protect the travelers who seroconverted from clinical malaria. None of the travelers to low-endemic areas where chemoprophylaxis is not recommended contracted malaria, so there is no reason to adapt the Dutch national guidelines. Similar prospective studies with larger numbers to specific destinations are needed to make more specific destination- dependent advice about the use of chemoprophylaxis.
The authors thank the medical doctors and nurses of the Public Health Service Amsterdam for their assistance in subject inclusion and data collection, in particular Gijs Baaten and Joan Kint for their extensive work in this study; Floor Elfrink, Bart Maertzdorf and Gerben Rienk Visser for facilitating data management; Jane Whelan for statistical input; Martien Borgdorff for his advice and critical review of the paper; Susan T. Landry for editorial review.
Commented on the manuscript: WR RS. Conceived and designed the experiments: AvdH GS. Analyzed the data: SB. Contributed reagents/materials/analysis tools: WR RS. Wrote the paper: SB AH GS.
- 1. Hay SI, Guerra CA, Tatem AJ, Noor AM, Snow RW (2004) The global distribution and population at risk of malaria: past, present, and future. Lancet Infect Dis 4: 327–336.
- 2. World Health Organization (2012) Malaria factsheet. Available: http://www.who.int/mediacentre/factsheets/fs094/en/index.html. Accessed 23 Jan 2013.
- 3. Jelinek T, Bluml A, Loscher T, Nothdurft HD (1998) Assessing the incidence of infection with Plasmodium falciparum among international travelers. Am J Trop Med Hyg 59: 35–37.
- 4. Chen LH, Keystone JS (2005) New strategies for the prevention of malaria in travelers. Infect Dis Clin North Am 19: 185–210.
- 5. Williams CJ, Jones J, Chiodini P (2007) High case-fatality from falciparum malaria in UK travellers returning from The Gambia: a case series. Travel Med Infect Dis 5: 295–300.
- 6. Behrens RH, Carroll B, Hellgren U, Visser LG, Siikamaki H, et al. (2010) The incidence of malaria in travellers to South-East Asia: is local malaria transmission a useful risk indicator? Malar J 9: 266.
- 7. Schlagenhauf P, Petersen E (2008) Malaria chemoprophylaxis: strategies for risk groups. Clin Microbiol Rev 21: 466–472.
- 8. van Rijckevorsel GG, Sonder GJ, Geskus RB, Wetsteyn JC, Ligthelm RJ, et al. (2010) Declining incidence of imported malaria in the Netherlands, 2000–2007. Malar J 9 300 300.
- 9. Gyorkos TW, Svenson JE, Maclean JD, Mohamed N, Remondin MH, et al. (1995) Compliance with antimalarial chemoprophylaxis and the subsequent development of malaria: a matched case-control study. Am J Trop Med Hyg 53: 511–517.
- 10. Kain KC, Harrington MA, Tennyson S, Keystone JS (1998) Imported malaria: prospective analysis of problems in diagnosis and management. Clin Infect Dis 27: 142–149.
- 11. Jelinek T, Schade LC, Siikamaki H, Myrvang B, Chiodini P, et al. (2008) European cluster of imported falciparum malaria from Gambia. Euro Surveill 13: 19077.
- 12. Leder K, Wilson ME, Freedman DO, Torresi J (2008) A comparative analysis of methodological approaches used for estimating risk in travel medicine. J Travel Med 15: 263–272.
- 13. Cobelens FG, Verhave JP, Leentvaar-Kuijpers A, Kager PA (1998) Testing for anti-circumsporozoite and anti-blood-stage antibodies for epidemiologic assessment of Plasmodium falciparum infection in travelers. Am J Trop Med Hyg 58: 75–80.
- 14. DePetrillo JC, Singer C, Bergagnini IA, Kolakowski P, Edwards B, et al. (2010) Assessment of adherence to atovaquone-proguanil prophylaxis in travelers. J Travel Med 17: 217–220.
- 15. Goodyer L, Rice L, Martin A (2011) Choice of and adherence to prophylactic antimalarials. J Travel Med 18: 245–249.
- 16. Huzly D, Schonfeld C, Beuerle W, Bienzle U (1996) Malaria Chemoprophylaxis in German Tourists: A Prospective Study on Compliance and Adverse Reactions. J Travel Med 3: 148–155.
- 17. Nothdurft HD, Jelinek T, Bluml A, von SF, Loscher T (1999) Seroconversion to circumsporozoite antigen of Plasmodium falciparum demonstrates a high risk of malaria transmission in travelers to East Africa. Clin Infect Dis 28: 641–642.
- 18. Hogh B, Clarke PD, Camus D, Nothdurft HD, Overbosch D, et al. (2000) Atovaquone-proguanil versus chloroquine-proguanil for malaria prophylaxis in non-immune travellers: a randomised, double-blind study. Malarone International Study Team. Lancet 356: 1888–1894.
- 19. Knappik M, Peyerl-Hoffmann G, Jelinek T (2002) Plasmodium falciparum: use of a NANP19 antibody-test for the detection of infection in non-immune travellers. Trop Med Int Health 7: 652–656.
- 20. Cobelens FG, Leentvaar-Kuijpers A (1997) Compliance with malaria chemoprophylaxis and preventative measures against mosquito bites among Dutch travellers. Trop Med Int Health 2: 705–713.
- 21. United Nations Statistics Division (2011) Composition of macro geographical (continental) regions, geographical sub-regions, and selected economic and other groupings. Available: http://unstats.un.org/unsd/methods/m49/m49regin.htm Accessed 2013 Jan 23.
- 22. LCR (2007) Landelijk Cordinatiecentrum Reizigersadvisering. National Coördination Centre for Traveller’s Health Advice: Malariaprofylaxe Bulletin, Amsterdam, The Netherlands: LCR (in Dutch).
- 23. Robert V, Roeffen W, Brasseur P, Aribot G, Verhave JP, et al. (2000) Anti-NANP antibody and treatment efficacy in patients with acute uncomplicated falciparum malaria attacks. Parasite Immunol 22: 589–593.
- 24. Lobel HO, Baker MA, Gras FA, Stennies GM, Meerburg P, et al. (2001) Use of malaria prevention measures by North American and European travelers to East Africa. J Travel Med 8: 167–172.
- 25. Petersen E, Ronne T, Ronn A, Bygbjerg I, Larsen SO (2000) Reported side effects to chloroquine, chloroquine plus proguanil, and mefloquine as chemoprophylaxis against malaria in Danish travelers. J Travel Med 7: 79–84.
- 26. Ropers G, Du Ry van Beest Holle, Wichmann O, Kappelmayer L, Stuben U, et al. (2008) Determinants of malaria prophylaxis among German travelers to Kenya, Senegal, and Thailand. J Travel Med 15: 162–171.
- 27. Resseguier N, Machault V, Ollivier L, Orlandi-Pradines E, Texier G, et al. (2010) Determinants of compliance with malaria chemoprophylaxis among French soldiers during missions in inter-tropical Africa. Malar J 9: 41.
- 28. Sagui E, Resseguier N, Machault V, Ollivier L, Orlandi-Pradines E, et al. (2011) Determinants of compliance with anti-vectorial protective measures among non-immune travellers during missions to tropical Africa. Malar J 10: 232.
- 29. Lobel HO, Phillips-Howard PA, Brandling-Bennett AD, Steffen R, Campbell CC, et al. (1990) Malaria incidence and prevention among European and North American travellers to Kenya. Bull World Health Organ 68: 209–215.
- 30. Overbosch D, Schilthuis H, Bienzle U, Behrens RH, Kain KC, et al. (2001) Atovaquone-proguanil versus mefloquine for malaria prophylaxis in nonimmune travelers: results from a randomized, double-blind study. Clin Infect Dis 33: 1015–1021.
- 31. Lalloo DG, Hill DR (2008) Preventing malaria in travellers. BMJ 336: 1362–1366.
- 32. Neave PE, Jones CO, Behrens RH (2010) A review of risk factors for imported malaria in the European African diaspora. J Travel Med 17: 346–350.
- 33. Orlandi-Pradines E, Penhoat K, Durand C, Pons C, Bay C, et al. (2006) Antibody responses to several malaria pre-erythrocytic antigens as a marker of malaria exposure among travelers. Am J Trop Med Hyg 74: 979–985.
- 34. Druilhe P, Pradier O, Marc JP, Miltgen F, Mazier D, et al. (1986) Levels of antibodies to Plasmodium falciparum sporozoite surface antigens reflect malaria transmission rates and are persistent in the absence of reinfection. Infect Immun 53: 393–397.
- 35. Behrens RH, Carroll B, Beran J, Bouchaud O, Hellgren U, et al. (2007) The low and declining risk of malaria in travellers to Latin America: is there still an indication for chemoprophylaxis? Malar J 6: 114.
- 36. Behrens RH, Carroll B, Smith V, Alexander N (2008) Declining incidence of malaria imported into the UK from West Africa. Malar J 7: 235.
- 37. Webster HK, Boudreau EF, Pang LW, Permpanich B, Sookto P, et al. (1987) Development of immunity in natural Plasmodium falciparum malaria: antibodies to the falciparum sporozoite vaccine 1 antigen (R32tet32). J Clin Microbiol 25: 1002–1008.
- 38. Roestenberg M, McCall M, Hopman J, Wiersma J, Luty AJ, et al. (2009) Protection against a malaria challenge by sporozoite inoculation. N Engl J Med 361: 468–477.
- 39. Sauerwein RW, Roestenberg M, Moorthy VS (2011) Experimental human challenge infections can accelerate clinical malaria vaccine development. Nat Rev Immunol 11: 57–64.