To compare the risk of stillbirth and miscarriage in a subsequent pregnancy in women with a previous Caesarean or vaginal delivery.
Systematic review of the published literature including seven databases: CINAHL; the Cochrane library; Embase; Medline; PubMed; SCOPUS and Web of Knowledge from 1945 until November 11th 2011, using a detailed search-strategy and cross-checking of reference lists.
Cohort, case-control and cross-sectional studies examining the association between previous Caesarean section and subsequent stillbirth or miscarriage risk. Two assessors screened titles to identify eligible studies, using a standardised data abstraction form and assessed study quality.
11 articles were included for stillbirth, totalling 1,961,829 pregnancies and 7,308 events. Eight eligible articles were included for miscarriage, totalling 147,017 pregnancies and 12,682 events. Pooled estimates across the stillbirth studies were obtained using random-effect models. Among women with a previous Caesarean an increase in odds of 1.23 [95% CI 1.08, 1.40] for stillbirth was yielded. Subgroup analyses including unexplained stillbirths yielded an OR of 1.47 [95% CI 1.20, 1.80], an OR of 2.11 [95% CI 1.16, 3.84] for explained stillbirths and an OR of 1.27 [95% CI 0.95, 1.70] for antepartum stillbirths. Only one study reported adjusted estimates in the miscarriage review, therefore results are presented individually.
Given the recent revision of the National Institute for Health and Clinical Excellence guidelines (NICE), providing women the right to request a Caesarean, it is essential to establish whether mode of delivery has an association with subsequent risk of stillbirth or miscarriage. Overall, compared to vaginal delivery, the pooled estimates suggest that Caesarean delivery may increase the risk of stillbirth by 23%. Results for the miscarriage review were inconsistent and lack of adjustment for confounding was a major limitation. Higher methodological quality research is required to reliably assess the risk of miscarriage in subsequent pregnancies.
Citation: O’Neill SM, Kearney PM, Kenny LC, Khashan AS, Henriksen TB, Lutomski JE, et al. (2013) Caesarean Delivery and Subsequent Stillbirth or Miscarriage: Systematic Review and Meta-Analysis. PLoS ONE 8(1): e54588. https://doi.org/10.1371/journal.pone.0054588
Editor: Philippa Middleton, The University of Adelaide, Australia
Received: May 30, 2012; Accepted: December 13, 2012; Published: January 23, 2013
Copyright: © 2013 O’Neill et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors have no support or funding to report.
Competing interests: The authors have declared that no competing interests exist.
A recent review reported that in high-income countries, one in every 200 pregnant women reaching 22 weeks gestation and beyond will have a stillborn baby . The UK has one of the highest stillbirth rates of high-income countries with only France and Australia ranking higher . There were 4,100 stillbirths reported in the UK in 2009, a rate of 3.5 per 1,000 births, or 11 stillbirths every day. Globally, more than three million pregnancies result in a stillbirth annually, the majority arising in developing countries . Reform of the classification of stillbirths is urgently needed, particularly the criteria for recording the cause of death and other vital information . No one classification system is commonly accepted, with varying definitions of stillbirth used by investigators, countries, health organisations, and classification schemes . Stillbirths can be defined according to gestational age at birth typically into early stillbirths (20–28 weeks gestation) and late stillbirths (>28 weeks) . Additionally stillbirths are classified into antepartum (death occurring before the onset of labour) or intrapartum (death during or after labour) . However, the primary method for classification of stillbirth is according to the apparent cause or associated obstetric disorders.
Stillbirths were first classified using the Aberdeen classification system based solely on available clinical information . This was followed by the British perinatal mortality survey in the 1950’s which used autopsy data ,  and most recently by the Wigglesworth criteria which were developed in the 1980’s and are the most widely used criteria to date . In Australia stillbirth is defined as any fetus born weighing more than 400 grams, or more than 20 weeks in gestation . In the United Kingdom, the definition of stillbirth is any fetus stillborn after 24 weeks gestation . Furthermore, many systems include both stillbirths and neonatal deaths. These variations in the lower gestational age limit the ability to compare findings from different studies. Earlier classification systems included only a few categories (congenital malformations, immaturity, and asphyxia) whilst more recent systems have tried to include more hierarchical information on fetal growth, placental changes, and maternal disorders , . Therefore, the use of conflicting classification systems, of which there are more than thirty in existence (with an additional twelve modifications of such systems) , may result in a deficit of essential information and a large proportion of unexplained stillbirths. In keeping with this, the contribution of unexplained stillbirths has been reported to be as high as 70% . For this reason, researchers and clinicians have strived to better classify stillbirths according to the aetiology and models of causation for more than two decades .
Important known causes of stillbirth common to developed and developing countries include placental insufficiency with fetal growth restriction , infection, pre-eclampsia, congenital abnormalities, placental abruption and umbilical cord accidents , –. In addition, short inter-pregnancy intervals, prior stillbirths and a history of adverse pregnancy outcomes have all been associated with increases in stillbirth risk in developing countries . Several risk factors for stillbirth have been identified, including primiparity, advanced maternal age, high BMI, maternal conditions such as pre-eclampsia, diabetes and hypertension, low educational attainment and socioeconomic status , , although the exact cause of stillbirth is often unknown , .
Spontaneous miscarriage (before 24 weeks gestation) is the most common early pregnancy complication with miscarriage rates ranging between 10% and 15% of recognised pregnancies , . Some studies report that approximately one in five pregnancies will end in a miscarriage –. This number would be even greater if very early pregnancy losses or missed miscarriages are included, with rates of over 33% reported , , . Similar to stillbirth, there have been many definitions for miscarriage but the most accepted and widely used is the World Health Organization’s (WHO) definition developed in 1977 . Following this, miscarriage is defined as “the expulsion or extraction from its mother of an embryo or fetus weighing 500 g or less”. Miscarriage can be further sub-classified into early miscarriage (6±12 weeks gestation) or late miscarriage (12±24 weeks gestation) . Therefore, variability in the definitions of miscarriage and stillbirth may affect the precision of recordings in registration systems, community and hospital surveys, together with those for measurement and comparison .
Chromosomal aneuploidies are reported to account for about 50–70% of miscarriages ,  (the commonest being monosomy X and trisomy 16), followed by thrombophilia . Risk factors for miscarriage include caffeine consumption, alcohol and drug use, previous induced abortions and uterine defects . Stillbirth and miscarriage share several risk factors including smoking –, advanced maternal age –, history of pregnancy loss , , , , and body mass index (BMI) , , –. History of Caesarean delivery has been implicated as a risk factor for both stillbirth  and miscarriage –, however, to date, evidence is conflicting , . The underlying mechanisms for an association between Caesarean delivery and stillbirth and miscarriage are unclear but may be related to placental abnormalities. However, often these adverse events occur with no obvious underlying cause .
Understanding potential long-term adverse effects associated with Caesarean delivery is essential given the exponential rise in Caesarean rates over the past three decades –. Caesarean rates currently range from over 25% in the UK  and 35% in the USA , , to over 40% in certain Latin American countries including Brazil, Chile and Argentina . The National Institute for Health and Clinical Excellence (NICE) guidelines , which were recently updated and give women the choice to request a Caesarean delivery without medical necessity, may lead to an increase in already high Caesarean rates. The aim of this systematic review is to examine the association between Caesarean delivery and subsequent risk of stillbirth and miscarriage.
Materials and Methods
The main objective of this systematic review and meta-analysis is to synthesise the available published literature to date on the relationship between prior Caesarean delivery and risk of stillbirth or miscarriage in the subsequent delivery and to report an estimate of the increase in odds of stillbirth or miscarriage following a Caesarean delivery.
The outcomes of interest in this review are stillbirth (explained, unexplained, antepartum or intrapartum) and miscarriage following a Caesarean delivery.
In accordance with the preferred reporting items for systematic reviews and meta-analyses statement (PRISMA) , we conducted a systematic review and meta-analysis of the published literature (without language or date restrictions). We selected potentially eligible studies published between 1945 up until November 11th 2011, from CINAHL, the Cochrane Library, Embase, Medline, PubMed, SCOPUS and Web of Knowledge databases with the following combined text and Medical Subject Headings (MeSH) including the exposure, outcome and study design (#Caesarean section AND #stillbirth OR #miscarriage AND #Case-control OR #Cohort study OR #Cross-sectional (Appendix S1). We supplemented our electronic search by cross-checking the reference lists of all identified studies. We included studies which published quantitative estimates of the association between mode of delivery and stillbirth or miscarriage. Eligibility criteria for inclusion in the meta-analysis included:
- Data were from an original study (i.e. no review articles, editorials or commentaries).
- Cohort, cross-sectional or case-control studies, in which mode of delivery in the previous pregnancy was reported and stillbirth or miscarriage in the subsequent pregnancy were the outcomes of interest.
- No strict definition of stillbirth or miscarriage was followed in the review. It was necessary only that there was a clear statement or understanding that “stillbirth” or “miscarriage” was the outcome of interest in each eligible study.
- Reporting of relative risk (RR), odds ratio (OR) or hazard ratio (HR) (or adequate data in order to compute these parameters), of mode of delivery associated with stillbirth or miscarriage.
Study and Data Collection Processes
Titles and abstracts of studies retrieved from the search strategy were reviewed using the appropriate inclusion and exclusion criteria. The full text article was obtained for all potentially eligible studies for further appraisal.
Using a data abstraction form, two assessors (SMON, RAG) individually selected data on study design, year of study, mode of delivery, stillbirth, miscarriage and potential confounding variables including smoking, maternal age, history of miscarriage or stillbirth and BMI. Discrepancies in data abstraction between assessors were resolved through consensus.
Our principal analysis investigated the overall risk of stillbirth or miscarriage in women with previous Caesarean delivery versus previous vaginal delivery. Pooled estimates across studies were obtained by means of random-effect models. Studies were weighted according to an estimate of statistical size defined as the inverse of the variance of the OR. We generated a funnel plot of the overall OR and a standard error (SE) to assess publication bias for each primary outcome. For stillbirth, 10 out of the 11 eligible studies reported adjusted estimates and these are reported in the meta-analysis. Where data were presented in a way that could not be included in a meta-analysis, results of the studies are presented individually. For miscarriage, only one study out of the eight eligible studies reported adjusted estimates and therefore it was decided that a meta-analysis would not be appropriate and as a result the study estimates are presented individually.
We estimated separate ORs for studies which reported an adjusted estimate for explained stillbirths, for unexplained stillbirths and for studies where cause of stillbirth was not specified or reported. In addition, we separately analysed studies including antepartum stillbirths only. Subgroup analyses by cause and timing of stillbirth is important in terms of confounding and estimating the role of unexplained stillbirths in any potential association.
We undertook two sensitivity analyses in the stillbirth meta-analysis. First, we estimated the pooled OR by study design (cohort, cross-sectional). This was considered important as various types of study designs may differ in methodological quality. For example, cohort studies would generally have a much larger sample size, thereby generating more statistical power and potentially less biased estimates compared to smaller case-control studies. Second, sensitivity analyses by parity (primiparous versus multiparous) were performed. This was important to assess the degree of confounding by number of previous pregnancies. Analyses were performed using SAS 9.2 (SAS Institute Inc., Cary, NC), and the meta-analysis was conducted using Review Manager version 5.1 software .
Heterogeneity between studies was examined by assessing differences in study characteristics including study setting (country, origin), study design (case control, cohort, cross-sectional), sampling frame (institutional or population-based), and definition of the outcome measure used. We assessed the degree of variability amongst studies attributable to between-study heterogeneity with the I2 statistic. Thresholds for the interpretation of I2 as recommended by the Cochrane Handbook for Systematic Reviews were followed in this review . An I2 value of 0% to 40% suggests heterogeneity might not be important; 30% to 60% represents moderate heterogeneity; 50% to 90% represents substantial heterogeneity; and 75% to 100% represents considerable heterogeneity. According to the Cochrane Handbook, the importance of the I2 value is dependent on the magnitude and direction of effects as well as the strength of the evidence for heterogeneity (Chi-squared test P-value, 95% confidence interval for I2).
Population Attributable Risk (PAR).
The population attributable risk (PAR) is the proportion of the incidence of disease in the population (exposed and unexposed) that is due to the exposure. The PAR of stillbirth is an estimate of the total number of cases of stillbirth in the population that can be attributed to a particular exposure, in this instance prior Caesarean delivery. The PAR was calculated according to a formula used by Last et al.  including the adjusted estimates; , where P is the proportion of total population with the exposure. Population attributable risk percent (PAR %) which is the percent of the incidence of a disease in a population that is due to exposure was also calculated using the following formula: . PAR and PAR% were calculated for all stillbirths in the total population as well as for unexplained stillbirths only.
Two reviewers (SMON, RAG) using a quality assessment tool based on six different types of bias common in observational studies (selection, exposure, outcome, analytic, attrition and confounding) assessed the overall study quality. This bias classification tool has been described in detail elsewhere , . Study bias was classified as minimal, low, moderate or high according to the degree of expected bias present for each of the six different types of bias and an overall likelihood of bias based on the total of the six different types of bias measured was reported.
We retrieved 4,619 non-duplicated studies (Figure 1), of which 41 included data for the association between mode of delivery and stillbirth and 23 included data for the association between mode of delivery and spontaneous miscarriage. The full text for these articles was reviewed for eligibility. The most frequent reason for study exclusion was absence of the outcome or the exposure of interest or study designs other than those cited in the inclusion criteria, followed by letters, reviews or editorials. For the stillbirth review, two studies ,  were excluded to avoid duplication as the data used in each came from the same Scottish database as a later study , which is included in the final review. Ten studies were eligible for inclusion, and one additional study was identified from cross-checking the reference lists, yielding a total of eleven studies for inclusion in the stillbirth review, nine cohort studies , – and two cross-sectional studies , . For the miscarriage review, five studies met the inclusion criteria, and three additional studies were identified from cross-checking of the reference lists, yielding a total of eight articles for inclusion in the review; seven cohort studies , , – and one case-control study . Hemminki et al.  reported on two separate cohorts, and consequently two different risk estimates representing each cohort are presented.
Characteristics of Studies Included in the Stillbirth Review
A summary of the study characteristics can be found in Table 1. The definition of stillbirth used varied by study with different cut-offs for gestational age and birth weight. One third of the studies defined stillbirth from an early gestational age (> = 20 weeks) , ,  or (> = 22 weeks) whilst two thirds used a later gestation (> = 23 weeks) , , (> = 24 weeks) , ,  and (> = 28 weeks) . Minimum birth weight defined as 400 g , , 500 g  or 1000 g  was used by four studies. Six studies included antepartum stillbirths , , , , , . All but one study  excluded women with multiple births (twins or greater) and four studies excluded deaths of fetuses as a result of congenital anomalies , , , . Ten of the included studies were conducted in high-income countries including: the UK , , Germany , , Australia , , Israel , the USA ,  and Canada  and one study was conducted in a low-income country, Nigeria . Ascertainment of stillbirth was confirmed in the studies through one or more of the following methods: hospital database(s) , –, , , patient charts , ,  or nationwide registers . Four studies used the WHO International Classification of Disease (ICD) codes , , , . The causes of stillbirth were identified in two studies ,  using Wigglesworth’s classification system , . Another study  used more recent criteria by Whitfield  to classify stillbirth and two studies used autopsy , .
For the primary analysis, data were available on 1,961,829 pregnancies and 7,308 (0.37%) events. No matched groups were used in any of the eleven studies. Only one study did not adjust for confounders and is not included in the meta-analysis . A crude OR of 1.08 (95% CI 0.77, 1.52) for risk of subsequent stillbirth among women with a prior Caesarean section was reported. Most studies adjusted for the following potential confounders: maternal age, smoking, history of pregnancy loss, gestational age and parity. Adjustment for other potential confounders including BMI, socioeconomic status, marital status, maternal height, birth weight, medical complications such as diabetes or hypertension and race/ethnicity varied between the studies. Three studies reported a sample size or power calculation , , . A random-effects model is reported due to considerable heterogeneity between the studies in the fixed-effect model (I2 = 84.2%, P = >0.00001). The pooled adjusted OR of stillbirth among women with previous Caesarean delivery versus vaginal delivery was 1.23 (95% CI 1.08, 1.40) (Figure 2). Inspection of the funnel plot (Figure 3) did not indicate evidence of publication bias.
Subgroup analysis by cause of stillbirth yielded an OR of 1.47 (95% CI 1.20, 1.80) for studies including unexplained stillbirths, an OR of 2.11 (95% CI 1.16, 3.84) for the single study which reported an estimate for explained stillbirths and an OR of 1.12 (95%CI 0.97, 1.31) for those studies which included unspecified stillbirths (i.e. did not state whether the stillbirths were explained or unexplained and/or antepartum or intrapartum) (Figure 2). The Chi2 estimate to test for subgroups differences was 7.30 (P = 0.03). When studies which reported including antepartum stillbirths only were separately analysed, an OR of 1.27 (95% CI 0.95, 1.70, Chi2 38.87) was generated (Figure 4).
When the single cross-sectional study  was omitted, there was a small shift in the OR [from 1.23, 95% CI 1.08, 1.40 to 1.28, 95% CI 1.05, 1.56] (Figure 5). Separate analyses by parity produced an OR of 1.29 (95% CI 1.12, 1.49, Chi2 11.90) for primiparous women, and an OR estimate of 1.13 (95% CI 0.75, 1.72, Chi2 18.57) for multiparous women (Figure 6).
The PAR for all stillbirths in the total population attributed to previous Caesarean section was calculated to be 0.03551 per 100 and the PAR% estimated at 3.6%. PAR for unexplained stillbirths was 0.067 per 100 with a PAR% of 6.7% (data not shown).
Characteristics of Studies Included in the Miscarriage Review
A summary of the study characteristics can be found in Table 2. The definition of miscarriage used varied by study and information provided was limited in terms of the gestational age cut-off and minimum birth weight. Two studies defined miscarriage using ICD codes , . Miscarriage was simply defined as ‘spontaneous abortion’ or ‘miscarriage’ by the remaining studies. Five studies excluded women with multiple births (twins or greater) –. All of the included studies were conducted in high-income countries including: the USA , , Finland , Scotland , , , England  and Sweden . Miscarriage diagnosis was confirmed in the studies through one or more of the following methods: hospital database(s) , , , patient charts , interviewing of women , survey data  or nationwide registers , , . Two studies cited using the WHO ICD codes , . Two studies ,  divided the Caesarean group by indication (emergency or elective). For the other studies, it was assumed that the exposed group included all Caesareans. No study distinguished between early (6±12 weeks gestation) or late miscarriage (12±24 weeks gestation). Five studies used frequency matching by one or both of the following: age , , , ,  and date of delivery , , . Adjustment for potential confounders (marital status, deprivation, birth weight percentile, infant sex, maternal age, maternal height and method of induction) was only performed in one study  in the miscarriage review. None of the studies reported a sample size or power calculation. For the primary analysis of miscarriage, data were available on 147,017 women, of which 12,682 (8.6%) were reported to have experienced a miscarriage. Results of each study included in the miscarriage review are presented separately (Table 3) due to significant heterogeneity as a result of lack of adjustment for confounding. Two studies ,  reported a statistically significant increase in odds of miscarriage following Caesarean delivery by 32% and 22% respectively. However, such results must be interpreted with caution as adjustment for confounders was not possible. Only one study  reported a reduction in the odds of miscarriage following Caesarean delivery, however, this was not statistically significant.
Quality assessment of the included studies (Tables 4, 5) was based on a bias classification tool estimating six types of bias (Appendix S2). Overall, the risk of bias for the studies included in the stillbirth review was considered ‘minimal’ and ‘moderate’ for the miscarriage studies.
The characteristics of the included studies are shown in Table 1. All ten studies included in the stillbirth meta-analysis were from high income countries. In addition all sampled retrospective population-based cohorts using hospital or register-based databases. Variations in the definition of stillbirth used may account for some of the heterogeneity observed. Definitions ranged from greater than 20 weeks to greater than 28 weeks and included all, explained or unexplained stillbirths. The I2 statistic was used to measure statistical heterogeneity and varied from 0% to 89%. Heterogeneity due to cause and timing of stillbirth used and by study design and parity may explain some or all of the observed heterogeneity.
The characteristics of the included studies are shown in Table 2. All eight studies were from high income countries. Variations in the definition of miscarriage used may account for some of the heterogeneity observed as there was limited information available on gestation and birth weight. Furthermore, only one study adjusted for confounding.
The overall findings of the meta-analysis suggest that women with a previous Caesarean delivery have a 23% increased odds of subsequent stillbirth compared to women with a previous vaginal delivery. The significant effect of Caesarean delivery on stillbirth was present in the overall meta-analysis and persisted in the subgroup analysis by cause of stillbirth (explained, unexplained) as well as the sensitivity analyses by study design (cohort studies only) and parity (primiparous women only). A reduction of 0.036 stillbirths per 100 population (exposed and unexposed) is expected if women were not exposed to a Caesarean delivery (PAR = 0.036 per 100). This represents a 3.6% reduction of the incidence in the population (PAR% = 3.6%). For unexplained stillbirths only, such a reduction represents a 6.7% decrease in the incidence in the population (PAR% = 6.7%). The results for subsequent risk of miscarriage are less pronounced and due to lack of adjustment for confounders no meta-analysis was conducted. The single study in the miscarriage analysis that included adjustment for confounders did not report a significant association. This would suggest that confounding may explain some or all of this increased risk. Overall the results of this systematic review and meta-analysis underscore the importance of further research into the association between mode of delivery and risk of subsequent miscarriage or stillbirth.
To date, there are major gaps in the understanding of the aetiology of stillbirth and miscarriage. Prior to this review, the association between previous Caesarean delivery and stillbirth or miscarriage in subsequent pregnancies was unclear. Flenady et al.  undertook a meta-analysis to investigate potential risk factors for stillbirth in high income countries and also found an increased odds of stillbirth following Caesarean delivery, with unexplained stillbirths attributable to an even greater increase in odds, similar to the findings of this systematic review and meta-analysis. Given the increasing use of Caesarean delivery for reasons including maternal choice, increased maternal age, fear of litigation among clinicians and repeat Caesareans, even a small increase in risk would have important implications at a population level. Despite surgical advances, Caesarean delivery continues to be associated with a significantly increased risk of maternal morbidity including haemorrhage, chronic pain, pelvic adhesion, sub fertility, placenta accrete  and death compared with vaginal delivery , , as well as an increased risk of perinatal morbidity  in subsequent deliveries. Nevertheless, it is also important to comment on the potential benefits associated with Caesarean deliveries including reduced urinary incontinence  and its necessity in emergency situations such as breech presentation , prematurity  and dystocia . Caesarean delivery today is a much safer operation due to advances in anaesthesia, antibiotics, surgical training and blood transfusion . However, some of these indications including breech presentation for Caesarean delivery in a previous pregnancy may confer an increased risk of stillbirth or miscarriage in the next pregnancy. Therefore confounding by indication in the previous pregnancy may explain the increased risk generated in the overall pooled analysis. However the persistence of the association between mode of delivery and stillbirth in sensitivity analyses including by parity suggests that the association may be real. Moreover, with such an exponential rise in Caesarean delivery, any potential association between mode of delivery and adverse pregnancy outcomes such as stillbirth and miscarriage is of major public health and health policy importance in the future.
Strengths and Limitations of the Review
A major strength of the review includes the comprehensive literature search which included seven databases using a wide-ranging collection of search terms and two reviewers. The heterogeneity between included studies was examined using appropriate sensitivity analyses and overall pooled estimates were generated to quantify the effect of Caesarean delivery on subsequent stillbirth and miscarriage. However, several limitations were found in some of the included observational studies. Data deficits or data of poor and variable quality existed with varying definitions of both outcomes used. Only four studies for stillbirth , , ,  and two for miscarriage ,  used of the International Classification of Disease (ICD) coding. Two studies referred to Wigglesworth’s criteria for stillbirth ,  and one study used Whitfield’s criteria , both of which have their own strengths and weaknesses. As previously discussed, there was a wide variation in the definition of stillbirth as well as miscarriage used in the studies included in this review. This is to a degree due to varying definitions of stillbirth and miscarriage in different countries as well as the definitions changing over time. Such variation may as a result over or under estimate stillbirth risk, conditional on whether the association is dependent on gestational age or not. No study in the miscarriage review included a definition of miscarriage according to gestation (commonly <20–22 weeks), and only two studies used ICD codes , . However, nationwide registry data was used by one large study in the stillbirth review  and three in the miscarriage review , , , with the majority having almost complete coverage, and examination of data recording quality. The other studies used patient records, interviews, survey data, and autopsy, which may be subject to errors in the form of miscoding or information bias. Small or inadequate sample sizes in the case-control studies with the largest effect estimates based on small sample sizes, as well as heterogeneous entry criteria may also limit the findings. Only three studies reported a power or sample size calculation in the stillbirth analysis and none were cited in the miscarriage analysis. However, the majority of included studies in both reviews were cohort studies with adequate power.
While adjustment for potential confounders varied across the studies, ten out of the 11 studies included in the stillbirth review adjusted for the main confounders. However, only one study adjusted for confounders in the miscarriage review and no meta-analysis was performed as a result. The quality of the outcome data in the retrospective studies may also be subject to bias in the form of under reporting of the events in the hospital records, recall bias and measurement bias. Selection of an appropriate, unbiased comparison group in case-control studies may also bias results in any direction.
Conclusions and Implications
The overall findings would suggest that women who previously delivered by Caesarean have a 23% increased risk of subsequent stillbirth compared to women who have previously delivered vaginally. However, studies included in the miscarriage review were of poor methodological quality and overall the results are inconsistent. The results must therefore be interpreted with caution. There are a multitude of medical, social and personal factors affecting decisions regarding mode of delivery. Caesarean delivery should be performed when medically required. However, the risk of subsequent adverse pregnancy outcomes, such as stillbirth and miscarriage, should not only influence medical decision-making, but also enter the patient and clinician discourse. These results are timely given the recent revision of the NICE guidelines, which for women requesting a Caesarean section, if after discussion and offer of support (including perinatal mental health support for women with anxiety about childbirth), a vaginal birth is still not acceptable, a Caesarean section is offered.
Risks and benefits to not only the current birth but also future pregnancies and births should be carefully considered. This particularly concerns primiparous women, who are likely to want more children. Research in the future should preferably use population-based data with lengthy follow-up. Clearly defined exposure and outcome criteria should be central to the research and information on potential key confounders would be vital for investigating any risks related to Caesarean delivery. A universal definition across all countries for stillbirth and miscarriage is recommended, for the recording of vital statistics and for research to be more comparative and of higher impact and better methodological quality.
Search terms used to search CINAHL, the Cochrane Library, Embase, MEDLINE, PubMed, Scopus and Web of Knowledge to identify studies on the association between Caesarean section and risk of stillbirth or spontaneous miscarriage.
We would like to acknowledge the staff of the National Perinatal Epidemiology Centre (NPEC), the Department of Epidemiology and Public Health, the Anu Research Centre and the Irish Health Research Board (HRB) for their support on this project. We would also like to acknowledge Professor John Browne of the Department of Epidemiology and Public Health in University College Cork for his didactic postgraduate course ‘Systematic Reviews for the Health Sciences’.
Conceived and designed the experiments: SMO RAG LCK JEL TBH PMK ASK. Performed the experiments: SMO RAG LCK JEL TBH PMK ASK. Analyzed the data: SMO RAG LCK JEL TBH PMK ASK. Contributed reagents/materials/analysis tools: SMO RAG LCK JEL TBH PMK ASK. Wrote the paper: SMO RAG LCK JEL TBH PMK ASK.
- 1. Flenady V, Koopmans L, Middleton P, Frřen JF, Smith GC, et al.. (2011) Major risk factors for stillbirth in high-income countries: a systematic review and meta-analysis. The Lancet, 377(9774): p. 1331–1340.
- 2. Stanton C, Lawn JE, Rahman H, Wilczynska-Ketende K, Hill K (2006) Stillbirth rates: delivering estimates in 190 countries. The Lancet, 367(9521): p. 1487–1494.
- 3. Pattinson Robert C, Say L, Makin JD, Bastos MH (2005) Critical incident audit and feedback to improve perinatal and maternal mortality and morbidity. Cochrane database of systematic reviews, DOI: https://doi.org/10.1002/14651858.CD002961.pub2.
- 4. Silver RM, Varner MW, Reddy U, Goldenberg R, Pinar H, et al.. (2007) Work-up of stillbirth: a review of the evidence. American journal of obstetrics and gynecology, 196(5): p. 433–444.
- 5. Smith G,Fretts RC. (2007) Stillbirth. The Lancet, 370(9600): p. 1715–1725.
- 6. Goldenberg RL, McClure EM, Bann CM (2007) The relationship of intrapartum and antepartum stillbirth rates to measures of obstetric care in developed and developing countries. Acta Obstet Gynecol Scand, 86(11): p. 1303–9.
- 7. Saastad E, Frøen JF (2009) An evaluation of classification systems for stillbirth. BMC pregnancy and childbirth.
- 8. Butler NR, Bonham DG (1963) Perinatal mortality: the First Report of the 1958 British Perinatal Mortality Survey under the auspices of the National Birtway Trust Fund.
- 9. Wigglesworth J (1980) Monitoring perinatal mortality. A pathophysiological approach. Lancet, 2: p. 684–6.
- 10. Lahra MM, Gordon A, Jeffery HE (2007) Chorioamnionitis and fetal response in stillbirth. American journal of obstetrics and gynecology, 196(3): p. 229. e1–229: e4.
- 11. Collins JH (2002) Umbilical cord accidents: human studies. Semin Perinatol. 2002 Feb 26(1): 79–82.
- 12. Flenady V, Frøen JF, Pinar H, Torabi R, Saastad E, et al.. (2009) An evaluation of classification systems for stillbirth. BMC pregnancy and childbirth, 9(1): p. 24.
- 13. Gardosi J, Kady SM, McGeown P, Francis A, Tonks A (2005) Classification of stillbirth by relevant condition at death (ReCoDe): population based cohort study. BMJ, 331(7525): p. 1113–1117.
- 14. Frøen JF, Pinar H, Flenady V, Bahrin S, Charles A, et al.. (2009) Causes of death and associated conditions (Codac)–a utilitarian approach to the classification of perinatal deaths. BMC pregnancy and childbirth, 9(1): p. 22.
- 15. M Kady S, Gardosi J (2004) Perinatal mortality and fetal growth restriction. Best practice & research Clinical obstetrics & gynaecology, 18(3): p. 397–410.
- 16. Simpson LL (2002) Maternal medical disease: risk of antepartum fetal death. Seminars in Perinatology, 26: p. 42–50.
- 17. Weiss HB, Songer TJ, Fabio A (2001) Fetal deaths related to maternal injury. JAMA: the journal of the American Medical Association, 286(15): p. 1863.
- 18. Bendon RW. (2001) Review of Some Causes of Stillbirth. Pediatric and Developmental Pathology, 4(6): p. 517–531.
- 19. Flenady V, Middleton P, Smith GC, Duke W, Erwich JJ, et al.. (2011) Stillbirths: the way forward in high-income countries. The Lancet, 377(9778): p. 1703–1717.
- 20. McClure EM, Saleem S, Pasha O, Goldenberg RL (2009) Stillbirth in developing countries: a review of causes, risk factors and prevention strategies. Journal of Maternal-Fetal & Neonatal Medicine, 22(3): p. 183–190.
- 21. Cnattingius S, Lambe M (2002) Trends in smoking and overweight during pregnancy: prevalence, risks of pregnancy complications, and adverse pregnancy outcomes. Semin Perinatol, 26(4): p. 286–95.
- 22. Macintosh M, Fleming KM, Bailey JA, Doyle P, Modder J, et al.. (2006) Perinatal mortality and congenital anomalies in babies of women with type 1 or type 2 diabetes in England, Wales, and Northern Ireland: population based study. BMJ, 333(7560): p. 177.
- 23. Zinaman MJ, Clegg E, Brown CC, O’connor J, Selevan S (1996) Estimates of human fertility and pregnancy loss. Fertility and sterility, 65(3): p. 503.
- 24. Garcia-Enguidanos A, Calle M, Valero J, Luna S, Dominguez-Rojas V (2002) Risk factors in miscarriage: a review. European Journal of Obstetrics & Gynecology and Reproductive Biology, 102(2): p. 111–119.
- 25. Savitz DA, Hertz-Picciotto I, Poole C, Olshan AF (2002) Epidemiologic measures of the course and outcome of pregnancy. Epidemiologic reviews, 24(2): p. 91–101.
- 26. Maconochie N, Doyle P, Prior S, Simmons R (2007) Risk factors for first trimester miscarriage–results from a UK-population-based case–control study. BJOG: An International Journal of Obstetrics & Gynaecology, 114(2): p. 170–186.
- 27. Wilcox AJ, Weinberg CR, O’Connor JF, Baird DD, Schlatterer JP, et al.. (1988) Incidence of early loss of pregnancy. New England Journal of Medicine, 319(4): p. 189–194.
- 28. WHO (1977) WHO recommended definitions, terminology and format for statistical tables related to the perinatal period Acta Obstet Gynecol Scand, 56: p. 247–53.
- 29. Lashen H, Fear K, Sturdee D (2004) Obesity is associated with increased risk of first trimester and recurrent miscarriage: matched case–control study. Oxford, England, 19(7): p. 1644.
- 30. Woods R (2008) Long-term trends in fetal mortality: implications for developing countries. Bulletin of the World Health Organization, 86(6): p. 460–466.
- 31. Gupta N, Kabra M (2011) Genetic Causes of Recurrent Miscarriage. World Clinics Obstetrics and Gynecology: Recurrent Miscarriage: p. 76.
- 32. Regan L, Rai R (2000) Epidemiology and the medical causes of miscarriage. Best practice & research Clinical obstetrics & gynaecology, 14(5): p. 839–854.
- 33. Regan L, Rai R (2002) Thrombophilia and pregnancy loss. Journal of reproductive immunology, 55(1–2): p. 163–180.
- 34. Högberg L, Cnattingius S (2007) The influence of maternal smoking habits on the risk of subsequent stillbirth: is there a causal relation? BJOG: An International Journal of Obstetrics & Gynaecology, 114(6): p. 699–704.
- 35. Raymond EG, Cnattingius S, Kiely JL (1994) Effects of maternal age, parity, and smoking on the risk of stillbirth. BJOG: An International Journal of Obstetrics & Gynaecology, 101(4): p. 301–306.
- 36. Cnattingius S, Stephansson O (2002) The epidemiology of stillbirth. Seminars in Perinatology, 26: p. 25–30.
- 37. Sandahl B (1989) Smoking habits and spontaneous abortion. European Journal of Obstetrics & Gynecology and Reproductive Biology, 31(1): p. 23–31.
- 38. Blohm F, Fridén B, Milsom I (2008) A prospective longitudinal population-based study of clinical miscarriage in an urban Swedish population. BJOG: An International Journal of Obstetrics & Gynaecology, 115(2): p. 176–183.
- 39. Andersen AMN, Wohlfahrt J, Christens P, Olsen J, Melbye M (2000) Maternal age and fetal loss: population based register linkage study. BMJ, 320(7251): p. 1708–1712.
- 40. Jacobsson B, Ladfors L,Milsom I (2004) Advanced maternal age and adverse perinatal outcome. Obstetrics & Gynecology, 104(4): p. 727.
- 41. Huang L, Sauve R, Birkett N, Fergusson D, Van Walraven C (2008) Maternal age and risk of stillbirth: a systematic review. Canadian Medical Association Journal, 178(2): p. 165–172.
- 42. Atik RB, Hepworth-Jones BE, Doyle P (2010) Risk factors for miscarriage. Early Pregnancy: p. 9.
- 43. de La Rochebrochard E, Thonneau P (2002) Paternal age and maternal age are risk factors for miscarriage; results of a multicentre European study. Oxford, England, 17(6): p. 1649.
- 44. Reddy UM (2007) Prediction and prevention of recurrent stillbirth. Obstetrics and gynecology, 110(5): p. 1151–1164.
- 45. Robson SJ, Leader LR (2010) Management of subsequent pregnancy after an unexplained stillbirth. Journal of Perinatology, 30(5): p. 305–310.
- 46. Chu SY, Kim SY, Lau J, Schmid CH, Dietz PM, et al.. (2007) Maternal obesity and risk of stillbirth: a metaanalysis. American journal of obstetrics and gynecology, 197(3): p. 223–228.
- 47. Nohr EA, Bech BH, Davies MJ, Frydenberg M, Henriksen TB, et al.. (2005) Prepregnancy obesity and fetal death: a study within the Danish National Birth Cohort. Obstetrics & Gynecology, 106(2): p. 250.
- 48. Fedorcsák P, Storeng R, Dale PO, Tanbo T, Åbyholm T (2000) Obesity is a risk factor for early pregnancy loss after IVF or ICSI. Acta obstetricia et gynecologica Scandinavica, 79(1): p. 43–48.
- 49. Hemminki E. (1996) Impact of caesarean section on future pregnancy–a review of cohort studies. Paediatr Perinat Epidemiol, 10(4): p. 366–79.
- 50. Hemminki E (1991) Long term maternal health effects of caesarean section. J Epidemiol Community Health, 45(1): p. 24–8.
- 51. Hemminki E, Merilainen J (1996) Long-term effects of cesarean sections: ectopic pregnancies and placental problems. Am J Obstet Gynecol, 174(5): p. 1569–74.
- 52. Tower CL, Strachan BK, Baker PN (2000) Long-term implications of caesarean section. Journal of Obstetrics and Gynaecology, 20(4): p. 365–367.
- 53. Franz MB, Lack N, Schiessl B, Mylonas I, Friese K, et al.. (2009) Stillbirth following previous cesarean section in Bavaria/Germany 1987–2005. Arch Gynecol Obstet, 279(1): p. 29–36.
- 54. Matthews T, Crowley P, Chong A, McKenna P, McGarvey C, et al.. (2003) Rising caesarean section rates: a cause for concern? BJOG: An International Journal of Obstetrics & Gynaecology, 110(4): p. 346–349.
- 55. Mukherjee S (2006) Rising cesarean section rate. The Journal of Obstetrics and Gynecology of India, 56(4): p. 298–300.
- 56. AIHW (2011) Australia’s mothers and babies 2009. Perinatal statistics. Series no.25. Available: http://www.aihw.gov.au/publication-detail/?id=10737420870.
- 57. Thomas J, Callwood A, Brocklehurst P, Walker J (2000) The national sentinel caesarean section audit. BJOG: An International Journal of Obstetrics & Gynaecology, 107(5): p. 579–580.
- 58. Martin JA, Hamilton BE, Sutton PD, Ventura SJ, Mathews T, et al.. (2010) Births: final data for 2007. National vital statistics reports, 58(24): p. 1–125.
- 59. Althabe F, Sosa C, Belizán JM, Gibbons L, Jacquerioz F, et al.. (2006) Cesarean Section Rates and Maternal and Neonatal Mortality in Low-, Medium-, and High-Income Countries: An Ecological Study. Birth, 33(4): p. 270–277.
- 60. Belizán JM, Althabe F, Barros FC, Alexander S, Showalter E, et al.. (1999) Rates and implications of caesarean sections in Latin America: ecological study. BMJ, 319(7222): p. 1397.
- 61. NICE, 2011, Caesarean section: full guideline DRAFT National Insitute for Health and Clinical Excellence: London. p. 266.
- 62. Moher D, Liberati A, Tetzlaff J, Altman DG (2009) Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. BMJ, 339: p. b2535.
- 63. Cochrane, 2011, Review Manager (RevMan) [Computer program]. Version 5.1. Copenhagen: The Nordic Cochrane Centre.
- 64. Higgins JPT, Green S, Collaboration C (2008) Cochrane handbook for systematic reviews of interventions. Vol. 5: Wiley Online Library.
- 65. Last JM (2000) A dictionary of epidemiology: Oxford University Press, USA.
- 66. McDonald SD, Han Z, Mulla S, Murphy KE, Beyene J, et al.. (2009) Preterm birth and low birth weight among in vitro fertilization singletons: A systematic review and meta-analyses. European Journal of Obstetrics & Gynecology and Reproductive Biology, 146(2): p. 138–148.
- 67. McDonald SD, Murphy K, Beyene J, Ohlsson A (2005) Perinatel outcomes of singleton pregnancies achieved by in vitro fertilization: a systematic review and meta-analysis. Journal of obstetrics and gynaecology Canada: JOGC = Journal d’obstétrique et gynécologie du Canada: JOGC, 27(5): p. 449.
- 68. Smith G, Pell JP, Cameron AD, Dobbie R (2002) Risk of perinatal death associated with labor after previous cesarean delivery in uncomplicated term pregnancies. JAMA: the journal of the American Medical Association, 287(20): p. 2684.
- 69. Smith GC, Pell JP, Dobbie R (2003) Caesarean section and risk of unexplained stillbirth in subsequent pregnancy. Lancet, 362(9398): p. 1779–84.
- 70. Smith GCS, Shah I, White IR, Pell JP, Dobbie R (2007) Previous preeclampsia, preterm delivery, and delivery of a small for gestational age infant and the risk of unexplained stillbirth in the second pregnancy: a retrospective cohort study, Scotland, 1992–2001. American journal of epidemiology, 165(2): p. 194–202.
- 71. Kennare R, Tucker G, Heard A, Chan A (2007) Risks of adverse outcomes in the next birth after a first cesarean delivery. Obstet Gynecol, 109(2 Pt 1): p. 270–6.
- 72. Ohana O, Holcberg G, Sergienko R, Sheiner E (2011) Risk factors for intrauterine fetal death (1988–2009). The journal of maternal fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 24(9): p. 1079–83.
- 73. Salihu HM, Sharma PP, Kristensen S, Blot C, Alio AP, et al.. (2006) Risk of stillbirth following a cesarean delivery: black-white disparity. Obstet Gynecol, 107(2 Pt 1): p. 383–90.
- 74. Richter R, Bergmann RL,Dudenhausen JW (2007) Previous caesarean or vaginal delivery: which mode is a greater risk of perinatal death at the second delivery? Eur J Obstet Gynecol Reprod Biol, 132(1): p. 51–7.
- 75. Gray R, Quigley M, Hockley C, Kurinczuk J, Goldacre M, et al.. (2007) Caesarean delivery and risk of stillbirth in subsequent pregnancy: a retrospective cohort study in an English population. BJOG: An International Journal of Obstetrics & Gynaecology, 114(3): p. 264–270.
- 76. Reddy UM, Laughon SK, Sun L, Troendle J, Willinger M, et al.. (2010) Prepregnancy risk factors for antepartum stillbirth in the United States. Obstet Gynecol, 116(5): p. 1119–26.
- 77. Wood SL, Chen S, Ross S, Sauve R (2008) The risk of unexplained antepartum stillbirth in second pregnancies following caesarean section in the first pregnancy. BJOG, 115(6): p. 726–31.
- 78. Taylor LK, Simpson JM, Roberts CL, Olive EC, Henderson-Smart DJ (2005) Risk of complications in a second pregnancy following caesarean section in the first pregnancy: a population-based study. Med J Aust, 183(10): p. 515–9.
- 79. Olusanya BO, Solanke OA (2009) Predictors of term stillbirths in an inner-city maternity hospital in Lagos, Nigeria. Acta Obstet Gynecol Scand, 88(11): p. 1243–51.
- 80. Smith GC, Wood AM, Pell JP, Dobbie R (2006) First cesarean birth and subsequent fertility. Fertility and sterility, 85(1): p. 90–5.
- 81. Hemminki E, Graubard BI, Hoffman HJ, Mosher WD, Fetterly K (1985) CESAREAN-SECTION AND SUBSEQUENT FERTILITY - RESULTS FROM THE 1982 NATIONAL SURVEY OF FAMILY GROWTH. Fertility and sterility, 43(4): p. 520–528.
- 82. Hall MH, Campbell DM, Fraser C, Lemon J (1989) Mode of delivery and future fertility. Br J Obstet Gynaecol, 96(11): p. 1297–303.
- 83. Hemminki E (1986) Effects of cesarean section on fertility and abortions. Journal of Reproductive Medicine for the Obstetrician and Gynecologist, 31(7): p. 620–624.
- 84. Mollison J, Porter M, Campbell D, Bhattacharya S (2005) Primary mode of delivery and subsequent pregnancy. BJOG: An International Journal of Obstetrics & Gynaecology, 112(8): p. 1061–1065.
- 85. LaSala A, Berkeley A (1987) Primary cesarean section and subsequent fertility. American journal of obstetrics and gynecology, 157(2): p. 379.
- 86. Keeling J, MacGillivray I, Golding J, Wigglesworth J, Berry J, et al.. (1989) Classification of perinatal death. Archives of Disease in Childhood, 64(10 Spec No): p. 1345–1351.
- 87. Cole S, Hey E, Thomson A (1986) Classifying perinatal death: an obstetric approach. BJOG: An International Journal of Obstetrics & Gynaecology, 93(12): p. 1204–1212.
- 88. Whitfield C, Smith N, Cockburn F, Gibson A (1986) Perinatally related wastage–a proposed classification of primary obstetric factors. BJOG: An International Journal of Obstetrics & Gynaecology, 93(6): p. 694–703.
- 89. Silver RM (2010) Delivery After Previous Cesarean: Long-Term Maternal Outcomes. Seminars in Perinatology, 34(4): p. 258–266.
- 90. Lydon-Rochelle M, Holt VL, Easterling TR, Martin DP (2001) Cesarean delivery and postpartum mortality among primiparas in Washington State, 1987–1996. Obstetrics & Gynecology, 97(2): p. 169.
- 91. Hall MH, Bewley S (1999) Maternal mortality and mode of delivery. Lancet, 354(9180): p. 776.
- 92. Levine EM, Ghai V, Barton JJ, Strom CM (2001) Mode of delivery and risk of respiratory diseases in newborns. Obstetrics & Gynecology, 97(3): p. 439.
- 93. Chaliha C, Digesu A, Hutchings A, Soligo M, Khullar V (2004) Caesarean section is protective against stress urinary incontinence: an analysis of women with multiple deliveries. BJOG: An International Journal of Obstetrics & Gynaecology, 111(7): p. 754–755.
- 94. Hannah ME, Hannah WJ, Hewson SA, Hodnett ED, Saigal S, et al.. (2000) Planned caesarean section versus planned vaginal birth for breech presentation at term: a randomised multicentre trial. The Lancet, 356(9239): p. 1375–1383.
- 95. Hagan R, Benninger H, Chiflings D, Evans S, French N (1996) Very preterm birth–a regional study. Part 1: Maternal and obstetric factors. BJOG: An International Journal of Obstetrics & Gynaecology, 103(3): p. 230–238.
- 96. Gherman RB, Chauhan S, Ouzounian JG, Lerner H, Gonik B, et al.. (2006) Shoulder dystocia: the unpreventable obstetric emergency with empiric management guidelines. American journal of obstetrics and gynecology, 195(3): p. 657–672.
- 97. Jolly J, Walker J, Bhabra K (1999) Subsequent obstetric performance related to primary mode of delivery. BJOG: An International Journal of Obstetrics & Gynaecology, 106(3): p. 227–232.