Most reef-building corals in the order Scleractinia depend on endosymbiotic algae in the genus Symbiodinium for energy and survival. Significant levels of taxonomic diversity in both partners result in numerous possible combinations of coral-Symbiodinium associations with unique functional characteristics. We created and analyzed the first coral-Symbiodinium networks utilizing a global dataset of interaction records from coral reefs in the tropical Indo-Pacific and Atlantic Oceans for 1991 to 2010. Our meta-analysis reveals that the majority of coral species and Symbiodinium types are specialists, but failed to detect any one-to-one obligate relationships. Symbiont specificity is correlated with a host’s transmission mode, with horizontally transmitting corals being more likely to interact with generalist symbionts. Globally, Symbiodinium types tend to interact with only vertically or horizontally transmitting corals, and only a few generalist types are found with both. Our results demonstrate a strong correlation between symbiont specificity, symbiont transmission mode, and community partitioning. The structure and dynamics of these network interactions underlie the fundamental biological partnership that determines the condition and resilience of coral reef ecosystems.
Citation: Fabina NS, Putnam HM, Franklin EC, Stat M, Gates RD (2012) Transmission Mode Predicts Specificity and Interaction Patterns in Coral-Symbiodinium Networks. PLoS ONE 7(9): e44970. https://doi.org/10.1371/journal.pone.0044970
Editor: Sebastian C. A. Ferse, Leibniz Center for Tropical Marine Ecology, Germany
Received: December 30, 2011; Accepted: August 16, 2012; Published: September 18, 2012
Copyright: © Fabina et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Support was provided by the National Science Foundation (OCE-0752604 to RDG; OCE-1041673 to MJD; EF-0928987 to SJS; EF-0553768 to NCEAS; 04-17412 to MCR-LTER; www.nsf.gov), the United States Environmental Protection Agency (FP-917096 to ECF; www.epa.gov), start up funds from the Dean of the College of Biological Sciences to SJS, a postdoctoral fellowship to MS from the AIMS-CSIRO-UWA collaborative agreement, and the University of California Davis Center for Population Biology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Scleractinian corals, the foundation of coral reefs, are extremely sensitive to global changes , . The condition and function of the coral holobiont is intimately tied to the identity of its symbiotic partners, which enhance or constrain coral responses to environmental stressors. Thus, there is an increasing need to study the symbiome – the collection of species with enduring associations within the physical limits of the coral colony . Temperature anomalies are generally believed to present the largest threat to coral persistence , as they cause corals to disassociate from their symbiotic partners, particularly when coupled with UV stress . This process leads to coral bleaching and, in extreme or persistent cases, colony mortality.
Most scleractinian corals depend on endosymbiotic dinoflagellates in the genus Symbiodinium for nutrition and survival . The coral-Symbiodinium association is considered mutualistic, with corals receiving photosynthetic carbon and Symbiodinium receiving nitrogen, phosphorus, and carbon dioxide. Indeed, Symbiodinium commonly provide a coral with over 90% of its energetic requirements. That said, associations can also be commensalistic, or even antagonistic, depending on partner identity and environmental conditions , , . Complicating matters is the diversity present in both guilds: there are hundreds of coral species and Symbiodinium types, with known diversity rapidly increasing with each new field study . Currently, there are nine recognized evolutionary lineages of Symbiodinium, clades A through I .
Symbiodinium clades are thought to have general functional characteristics that affect coral responses to a range of environmental variables. Clade B and D Symbiodinium are tolerant of temperature extremes , while clade A symbionts produce amino acids that protect against UV radiation , . Clade D is considered the most tolerant of disturbances and is thought to thrive in marginal environments because of a weedy or opportunistic life history . Indeed, corals with clade D are much more common in thermally disturbed reefs or pools , . However, there appears to be a tradeoff between short-term responses to temperature anomalies and long-term survival. While clade D continues photosynthesis during and after temperature stress , the presence of clade C is correlated with higher relative growth and lower relative mortality, both in juvenile and adult corals , . Understanding the functional differences between Symbiodinium and the implications for coral survival is critical to forecasting coral responses to the increases in anomalous ocean temperatures predicted with climate change.
The potential for corals to respond to environmental changes is a function of partner identity, thus the ability of corals to interact with functionally diverse assemblages of symbionts could enhance their resilience. This line of thinking led to the Adaptive Bleaching Hypothesis , , which posits that under adverse environmental settings, the ability to interact with diverse partners allows corals to shift (acquire new symbiont partners from the environment) or shuffle (adjust the relative abundances of existing partners) their symbiont assemblages to enhance performance . Indeed, a diverse Symbiodinium and microbial assemblage provides more consistent and diverse services in anemones and sponges , .
On the other hand, corals with obligatory symbiotic relationships that are functionally superior may be more environmentally resilient. Corals transmit Symbiodinium either vertically, from parent colony to egg or brooded larvae, or horizontally, where propagules or new recruits acquire symbionts from the environment . Vertical transmission is expected to increase symbiotic fidelity due to the coupling of coral and Symbiodinium fitness . Thus, corals that transmit symbionts vertically may have more specialized and mutualistic Symbiodinium, while horizontally transmitted symbionts may be more generalized and antagonistic . Despite the performance implications of coral specificity and transmission mode, no studies to date have explored the existing coral-Symbiodinium association data at multiple spatial scales for specificity or transmission trends.
The fitness of a coral is a function of its potential symbiotic partners, which in turn is a function of community patterns of interaction. To better understand the coral response to stress, it is necessary to address key questions related to the symbiotic unions including: Are corals predominantly specialists interacting with select Symbiodinium types, or generalists interacting with diverse groups of symbionts? Likewise, do Symbiodinium specialize or generalize in their partnerships with corals? Does symbiont transmission mode affect coral-Symbiodinium interaction patterns? One approach to addressing these complex yet fundamental questions in coral biology is network theory. Network theory is a framework for quantifying mutualistic interaction patterns ,  and their relationship to community stability , , . In mutualistic interaction networks, vertices represent species and edges represent the interaction between those species. Species are then connected if an interaction occurs between any individuals in their populations. We use network theory and general results from existing mutualistic network studies as an approach to understand coral responses to global changes, which are determined by these symbiotic characteristics , .
In this study, we explore coral-Symbiodinium associations using a long-term, global database of observed Symbiodinium associations. First, we characterize coral and Symbiodinium specificity at reef, ocean basin, and global scales. Second, we test whether transmission mode correlates with specificity of either host or symbiont. We additionally show how interaction patterns in coral-Symbiodinium networks compare to those in other mutualistic communities, and discuss their connection to community resilience (definitions reviewed in ).
Our analysis of coral-Symbiodinium interaction data revealed that there were many specialist coral species and Symbiodinium types and few generalists at reef, ocean basin, and global scales (Table 1, Fig. 1) (note that specialist and generalist are relative terms). We then restricted our analyses to the global network of 54 well-sampled corals and their 106 symbiont partners (Tables S1 and S2, Fig. 2; see methods). Grouping of corals into “specificity clusters” based on the number of interactions for a given coral and the mean number of interactions for its associated symbionts, resulted in four specificity clusters: (1) specialist corals with specialist Symbiodinium, (2) specialist corals with generalist Symbiodinium, (3) generalist corals with specialist Symbiodinium, and (4) intermediate corals with intermediate Symbiodinium (Fig. 3a). Corals in each cluster differed in the number of interactions for host and symbiont, as well as the variance in the number of interactions for associated symbionts (Fig. 3b). For example, corals in the specialist-generalist cluster associated with few Symbiodinium types, which interacted with many other hosts on average. Corals in the generalist-specialist cluster associated with many specialist symbionts, which had few alternative hosts. Corals in the former group interacted with both extreme specialists and extreme generalists, while corals in the latter group interacted only with specialists. Tantalizingly, there appeared to be two main strategies: (1) having many indirect interactions with other corals (through shared symbionts) on a gradient ranging through all of the generalist options, versus (2) having few indirect interactions with other corals and associating in a tight specialist-specialist interaction (Fig. 3a). Notably, there were no obligate relationships between a single coral species and a single Symbiodinium type at the global level.
Each group has many specialists and few generalists. Symbiodinium interaction numbers are logarithmically binned.
Black or filled elements denote interactions that have been observed (e.g., between Acropora cervicornis and type A3), while white or empty elements denote interactions that have not been observed (e.g., between Acropora cervicornis and type A4).
(a) The number of interactions for coral species and the mean number of interactions for their Symbiodinium partners. The nearly horizontal, dashed line is the statistical partition between horizontally and vertically transmitting corals. K-means cluster numbering corresponds with text description. (b) Boxplot showing variance in symbiont specificity for well-sampled corals in each cluster. Each coral has at least 30 interaction records and colors are consistent with (a). The number of symbiont partners per coral are in parentheses.
The mean number of interactions for a coral’s associated Symbiodinium was correlated with that coral’s transmission mode (see partition in Fig. 3a). Corals that interact with specialist symbionts on average (symbionts with fewer than ∼14 coral hosts) were almost always vertical transmitters, while corals that interact with generalist symbionts on average (symbionts with more than ∼14 coral hosts) were almost always horizontal transmitters (Wilcoxon; W = 377, mvert = 9.11, mhoriz = 21.81, nvert = 11, nhoriz = 35, p = 2.14 * 10−6, two-tailed). We explored the interaction preferences for Symbiodinium in the global network by comparing their realized and expected number of interactions with horizontally transmitting corals based on proportion of horizontal transmitters in the community. Symbiodinium types interacted with either horizontal or vertical transmitters much more than expected, and most symbionts interacted with only one group (Fig. 4a). Indeed, 46% of all Symbiodinium types in the dataset were found exclusively in vertical transmitters and 41% in horizontal transmitters. Generalist symbionts were much more likely to interact with both horizontal and vertical transmitters (Fig. 4b), while symbionts that were found only in horizontal or vertical transmitters were specialists. Vertically transmitting corals and horizontally transmitting corals had no appreciable difference in their mean number of interactions, despite the differences in their symbionts’ mean number of interactions (Wilcoxon; W = 139, mvert = 8.91, mhoriz = 6.77, p = .17, two-tailed).
(a) Symbiont preferences for (positive values) or against (negative) horizontally transmitting corals versus vertically transmitting corals. Zero indicates no preference, while 1 and −1 indicate coral hosts are only horizontal or vertical transmitters, respectively. (b) From left to right, symbionts which only interacted with vertical transmitters, interacted preferentially with vertical transmitters, interacted preferentially with horizontal transmitters, or only interacted with horizontal transmitters (x = kdiff). Numbers in parentheses indicate sample size in each group.
We explored a few common, unweighted network metrics to compare coral-Symbiodinium networks to other mutualistic networks (Table 1; see methods). The global network of well-sampled corals species had few realized interactions relative to the number of potential interactions (connectance = .07), had many more symbiont types than host species (web asymmetry = −.33), and was significantly nested (N*1 = 1.07, N*2 = .47, see Table 1). The ocean basin networks were also poorly connected and significantly nested, but the central and western Indo-Pacific had more coral species than symbiont types. Most reef-level networks (87.5%) had more coral (animal) species than Symbiodinium (plant) types, which, like animal-dominated pollination networks, seems to differ from many seed-dispersal and ant-myrmecophyte networks . Reef-level nestedness values (a measure of order) were not significant when compared to nestedness values generated with null models 1 and 2. Only 46% of reefs were significantly nested under null model 1 and 33% were significantly nested under null model 2.
We explored a global compilation of coral-Symbiodinium interactions , and found that both corals and Symbiodinium were composed of many specialists and few generalists. Further, we found that symbiont transmission mode was correlated with mean Symbiodinium partner specificity, and not coral specificity. Communities were generally partitioned between vertically transmitting corals and their symbiont partners.
Coral species with the potential to interact with functionally diverse Symbiodinium assemblages are hypothesized to be more responsive to global changes, i.e., the Adaptive Bleaching Hypothesis. Mutualistic network studies in other systems, such as pollination and seed-dispersal communities, find consistent network characteristics and correlations with resilience. In particular, generalists should be more resilient to disturbances than specialists. Thus, interpreting our results under the assumption that the ability to interact with functionally diverse Symbiodinium assemblages is beneficial, or with previous network findings in other ecosystems, leads to the same conclusion. Such patterns would suggest that Pocillopora damicornis and Stylophora pistillata, the two most generalist coral species in the global network, would be more resilient species and communities with many generalist species would be the most resilient.
However, it is also possible that the high degree of intimacy in these symbioses maximizes fitness through the co-evolution of high-fidelity partners . Tight functional integration may increase the range of environmental conditions in which a partnership is stable and stress resistant. Recent evidence  links generalist corals, such as Acropora and Pocillopora, to greater environmental sensitivity than specialists , , and suggests that corals with symbiotic specialization (e.g., massive Porites; ) may be more resilient to contemporary stressors . Vertical symbiont transmission also results in lower variation in partnerships between generations, and greater potential for co-evolution and increasing levels of symbiotic integration . Corals that are both specialists and vertical transmitters, such as Porites lobata and Montipora digitata, would then have more resilient populations.
Community resilience is also dependent on symbiont functional characteristics. For example, Symbiodinium types vary in their effects on coral growth rate , , carbon fixation and photosynthate transfer , . Further, a coral’s ability to cope with environmental stressors, such as temperature anomalies, is related to symbiont identity , , . Thus, Symbiodinium vary in the benefit they provide to their hosts depending on the community composition and environmental conditions. Unfortunately, not enough is known about symbiont functional characteristics to include this in our analysis . Nonetheless, differences between Symbiodinium types and unique coral-Symbiodinium pairings are certain to make coral resilience a function of both the number and diversity of symbiont partners. While we have quantitatively described the association potential of numerous coral species, definitively answering the resilience question is beyond the scope of our study and requires further investigation into functional response at Symbiodinium type level.
Our results provide evidence that contrasts with previous thoughts about coral-Symbiodinium relationships. First, many authors have suggested that corals are strict specialists due to the evidence that corals at local scales seem to interact with only one symbiont type [40, 41, but see ). Our results show that coral species have the potential to interact with many types (Fig. 3) and local specificity may be due to sampling methodology, or the limited spread of symbiont types (suggested by ). Notably, we found no obligate relationships between a coral species and a symbiont type at global scales. Porites rus was the only well-sampled coral to host a single symbiont type at global scales, but C15, its symbiont partner, has many alternative hosts. Second, previous authors have also suggested that vertically transmitting corals may be more specialist  and we provide evidence that coral specificity is unrelated to transmission mode (although it is related to symbiont specificity) (Fig. 3). Third, Wicks et al.  suggest that vertically and horizontally transmitting corals share symbionts, but it appears that symbionts are strongly partitioned (Fig. 4). Finally, previous studies suggest that vertically and horizontally transmitting corals have equivalent symbiont diversity at local scales . In contrast, we find that there are many more recognized Symbiodinium types transmitted in vertically transmitting corals (Fig. 4a). Our results are consistent with the findings of Stat et al  on a local scale in the southern Great Barrier Reef, perhaps because vertical transmission promotes diversification via isolation and maintained associations. These results suggest that future research should discern which patterns represent true barriers to association and which reflect biogeographic distributions (and thus the difference between a global or local perspective).
Our results are a product of a still incomplete understanding of coral-Symbiodinium interactions and symbiont diversity. Symbiodinium types have been described using several markers (nrDNA, rSSU, PLSU, ITS-1 and 2) and methodologies (DGGE, RFLP, SSCP, bacterial cloning, and direct sequencing) . The range of methods makes it difficult to create a comprehensive phylogeny within clades, and the identity, number, and ecological meaning of types is sometimes disputed , , 47. Researchers generally recognize DGGE ITS-2 types as identifying dominant Symbiodinium types, and our methods were chosen according to this interpretation. While our study provides new insights on data already collected, the next step is to use detailed high-resolution datasets to explore the sensitivity of our results to more detailed interaction data. Indeed, although we have the ability to create reef and ocean basin networks with GeoSymbio, we were forced to restrict most of our analyses to the global network of well-sampled coral species. Sampling intensity is variable between reefs and ocean basins and many network analyses are sensitive to species diversity or missing links.
Further studies with rigorous sampling designs will provide the detail necessary to explore the conservation implications of these patterns. Our dataset is an aggregation of coral-Symbiodinium interactions compiled across time and space  and from different researchers employing a variety of methodological approaches. However, we focus our analyses on the most well-sampled coral species. Our qualitative results differ only when severely undersampled species are included, and only affect the partition found in Fig. 3a. All other qualitative results are robust and increasing the number of samples or references needed for data inclusion only strengthens the observed patterns. However, certain network metrics are sensitive to species diversity, rare interactions, and other phenomena , ,  and future work is needed to include more sampling information and construct specific experiments designed to test network hypotheses. Based on these early results, the combination of network analyses with ecological, evolutionary, and environmental data appears to be a powerful quantitative approach for analyzing relationships between corals and their symbiotic partners.
We studied coral-Symbiodinium networks to quantify the specificity of corals and Symbiodinium at reef, ocean basin, and global scales, and to explore how specificity correlates with transmission mode. We used data from GeoSymbio, a database of interactions between Symbiodinium and their animal hosts from 79 publications between 1982 and 2010 . Interaction records were filtered to meet several criteria such that the final subset of data was aggregated from 21 publications with data collected between 1998 and 2009. First, only interaction records between hard corals (order Scleractinia identified at the genus and species level) and Symbiodinium (identified by subtype, which corresponds to differences in the Internal Transcribed Spacer Region 2 [ITS2] sequence) were included. Second, we restricted methodology to denaturing gradient gel electrophoresis (DGGE), which identifies the dominant Symbiodinium sequence within a coral sample . Third, we removed any aquarium records. Finally, records were removed if Symbiodinium sequence data were unavailable in GenBank, and together, these criteria minimized methodological differences.
We created coral-Symbiodinium networks at reef, ocean basin, and global scales to study interaction patterns. Coral species and Symbiodinium types were connected in a community’s interaction network if a coral species had been recorded as hosting a Symbiodinium type. Mutualistic interaction networks typically represent interactions between species, rather than genera or clades, and we follow this convention as closely as possible given the current taxonomy of Symbiodinium, where ITS2 types are thought to be most closely related to the species designation. As sampling intensities were not recorded within all references, we chose to analyze unweighted networks, which tracks the presence or absence (1 or 0) of interactions, rather than weighted networks, which additionally identifies the strength of interactions.
The interaction data were filtered to create reef-level or local networks. Geographical information for coral-Symbiodinium type associations in GeoSymbio followed a hierarchical framework modified from the Ocean Biogeographic Information System data schema (v1.1). To standardize community sampling effort based on the available data for sub-ocean basin scale networks, sample location levels were examined by A) state-region, B) subregion, and C) locale in the following fashion. Interaction data were included in our analyses if more than 30 interaction records were available at the state-region level, and each of the lower levels were subsequently examined if 30 interactions were available. Thirty interaction records were chosen as a lower threshold for community sampling because of a natural break between poorly sampled and well-sampled communities within GeoSymbio and to correspond to other mutualistic networks. Western Australia and the Exuma Islands, Bahamas were the only maximally-reduced state-region communities. Ocean basin networks were constructed by combining data for only the adequately sampled reef-level networks. The spatial boundaries for the western Indo-Pacific, central Indo-Pacific, and tropical Atlantic are consistent with the biogeographical realms in . La Paz, Oahu, and Zamami Island are not included in the ocean-level analysis due to the lack of data for the associated biogeographical realms.
We created global interaction networks by aggregating only the adequately sampled reef-level networks in GeoSymbio. The first global network included all of the data from the adequately sampled reef-level networks, while a second global network only included data from well-sampled corals. For inclusion in the well-sampled global interaction network, coral species needed to be sampled at least ten times from at least three different publications to minimize artifacts associated with sampling bias. Symbiodinium types needed to be recorded in at least one of the well-sampled coral species, as poorly-sampled corals could provide an inadequate picture of their symbiont populations, while rare Symbiodinium in well-sampled corals reflect an actual interaction trend. Despite the likelihood that increased sampling effort would increase the number of interactions reported, and that Symbiodinium diversity analyses are a much needed area of research globally, many specialist taxa are particularly well sampled and still have very few associated symbionts (see Montipora digitata and Porites cylindrica, Fig. 3b), displaying a trend rather than an artifact. Following the data filtering, we quantified the specificity of coral species for Symbiodinium types using descriptive statistics, and examined correlations with previously identified transmission mode (sensu ).
The specificity of coral hosts and their symbiont partners was further explored by clustering corals according to their degree (number of interactions) and the mean or median degree of their symbiont partners. We clustered corals using the k-means clustering algorithm, where the number of clusters, k, is predefined and the algorithm locates cluster means by optimizing the within cluster sum of squares relative to the across cluster sum of squares. The optimal number of clusters was selected conservatively by determining when less than 10% variance, or between cluster sum of squares to total sum of squares, was explained by an additional cluster. Cluster identity was robust to the use of mean or median symbiont degree. Correlations between coral transmission mode  and coral interaction number, and mean symbiont interaction number were tested using the Wilcoxon rank-sum test.
The partitioning between horizontally and vertically transmitted symbionts was tested using a modified network assortment metric . Assortment measures the degree to which members of distinct groups interact with one another more or less than expected. For each symbiont in the well-sampled global network, we determined its total number of interactions, k, its total number of interactions with horizontally transmitting corals, kh, and the proportion of horizontally transmitting corals in the community, ph. The expected number of interactions with horizontally transmitting corals given random interactions would then beand the difference between the expected and realized number of interactions is
Symbionts interact with horizontally transmitting corals more than expected if kdiff is positive, and interact with vertically transmitting corals more than expected if kdiff is negative. We then normalize kdiff based on the maximum or minimum possible value so that kdiff ranges from 1 (when symbionts only interact with horizontally transmitting corals and kh = k) to −1 (when symbionts only interact with vertically transmitting corals and kh = 0). Thus,
Coral-Symbiodinium network metrics were explored to determine whether general trends exist and how those trends compare to other mutualistic networks. Mutualistic interaction networks are characterized by properties that generally hold across wide geographic scales and interaction types (reviewed in , ). These include: significantly more species in one guild than the other (web asymmetry), a low number of realized interactions (connectance), high levels of nested organization (nestedness, see below), and many specialists versus few generalists. The consistency of these patterns has led to several hypotheses for their existence, including population sizes, optimal behavior, trait matching and barriers, and phylogenetic relationships . Most importantly, these traits may relate to the resilience - the ability to withstand perturbations - of a community under certain stressors , , .
We calculated web asymmetry, connectance, and nestedness, and created degree distributions for each network . Web asymmetry identifies networks with more coral types, c, than Symbiodinium types, s, and is equal to the number of coral species minus the number of Symbiodinium types, divided by the total number of types and species ((c − s)/(c + s)). Positive web asymmetry values indicate more coral species, while negative values indicate more Symbiodinium types. Connectance is the proportion of realized interactions or links, l, out of the total potential interactions, or the number of interactions divided by the number of coral species times the number of Symbiodinium types (l/(c * s)). Degree distributions are created by identifying the relative frequency, fd, of species with degree d, or a total of d interactions.
Nestedness is a measure of order in a community, where high levels of order indicate that the partners of specialists are subsets of the partners of generalists. We calculated nestedness using the overlay and decreasing fill (NODF) method via the nestednodf() function in the R package vegan (, using method = “NODF2”). Nestedness values range between 0 and 100, with greater values indicating greater nestedness. Nestedness values for the actual network were compared to null model expectations . The first null model holds the number of links in a network constant (connectance), while the second assumes the probability of two species interacting is equal to the average of their respective probabilities of interaction. We ran 1000 replicates for each null model and calculated relative nestedness to compare nestedness between networks . Relative nestedness is defined asWhere N is the NODF2 nestedness value for the actual network and Nr is the mean NODF2 nestedness value across all null model replicates.
Well-sampled coral species from the central Indo-Pacific and western Indo-Pacific. “Trans” = transmission mode; “Syms” = number of symbiont partners; “CIP” = found in central Indo-Pacific; “WIP” = found in western Indo-Pacific; “TA” = found in tropical Atlantic; “Samp” = number of samples; “Refs” = number of references. Transmission codes are “H” = horizontal, “V” = vertical, and “M” = mixed.
Well-sampled coral species from the tropical Atlantic. “Trans” = transmission mode; “Syms” = number of symbiont partners; “CIP” = found in central Indo-Pacific; “WIP” = found in western Indo-Pacific; “TA” = found in tropical Atlantic; “Samp” = number of samples; “refs” = number of references. Transmission codes are “H” = horizontal, “V” = vertical, and “M” = mixed.
GeoSymbio data. Data from GeoSymbio (https://sites.google.com/site/geosymbio/) used for analyses.
Transmission mode information. Transmission mode information from .
Schema for GeoSymbio data (Data S1).
Raw data are found at the GeoSymbio database (https://sites.google.com/site/geosymbio/). We thank JM Abbott, GM Coop, AE Douglas, AR Ives, X Pochon, SJ Schreiber, JJ Stachowicz, and three anonymous reviewers for insight and comments. This is HIMB contribution 1511 and SOEST contribution 8726.
Conceived and designed the experiments: NSF HMP ECF MS RDG. Performed the experiments: NSF HMP ECF MS. Analyzed the data: NSF. Contributed reagents/materials/analysis tools: RDG. Wrote the paper: NSF HMP ECF MS RDG.
- 1. Hughes TP, Baird AH, Bellwood DR, Card M, Connolly SR, et al. (2003) Climate change, human impacts, and the resilience of coral reefs. Science 301: 929–933.
- 2. Parmesan C (2006) Ecological and evolutionary responses to recent climate change. Annu Rev Ecol Syst 34: 637–669.
- 3. Gates RD, Ainsworth TD (2011) The nature and taxonomic composition of coral symbiomes as drivers of performance limits in scleractinian corals. J Exp Mar Bio Ecol 408: 94–101.
- 4. Hoegh-Guldberg O (2010) Coral reef ecosystems and anthropogenic change. Reg Environ Change 11: S215–S227.
- 5. Douglas AE (2003) Coral bleaching - how and why? Mar Poll Bull 46: 385–392.
- 6. Muscatine L, Porter J (1977) Reef corals: mutualistic symbioses adapted to nutrient-poor environments. Bioscience 27: 454–460.
- 7. Berkelmans R, van Oppen MJH (2006) The role of zooxanthellae in the thermal tolerance of corals: a “nugget of hope” for coral reefs in an era of climate change. Proc Royal Soc B 273: 2305–2312.
- 8. Stat M, Morris E, Gates RD (2008) Functional diversity in coral-dinoflagellate symbiosis. PNAS 105: 9256–9261.
- 9. Stat M, Gates RD (2011) Clade D Symbiodinium in scleractinian corals: a “nugget” of hope, a selfish opportunist, an ominous sign, or all of the above? J Mar Biol 730715: 1–9.
- 10. Stambler N (2011) Zooxanthellae: the yellow symbionts inside animals. In: Coral Reefs: an Ecosystem in Transition. Heidelberg: Springer.
- 11. Pochon X, Gates RD (2010) A new Symbiodinium clade (Dinophyceae) from soritid foraminifera in Hawai’i. Mol Phylo Evo 56: 492–497.
- 12. Knowlton N, Rohwer F (2003) Multispecies microbial mutualisms on coral reefs: the host as a habitat. Am Nat 162: S51–S62.
- 13. Baker AC (2003) Flexibility and specificity in coral-algal symbiosis: diversity, ecology, and biogeography of Symbiodinium. Annu Rev Ecol Syst 34: 661–689.
- 14. Baker AC, Starger CJ, McClanahan TR, Glynn PW (2004) Corals’ adaptive response to climate change. Nature 430: 741.
- 15. Oliver TA, Palumbi SR (2009) Distributions of stress-resistant coral symbionts match environmental patterns at local but not regional scales. Mar Ecol Prog Ser 378: 93–103.
- 16. Oliver TA, Palumbi SR (2011) Many corals host thermally resistant symbionts in high-temperature habitat. Coral Reefs 30: 241–250.
- 17. Rowan R (2004) Thermal adaptation in reef coral symbionts. Nature 430: 742.
- 18. Little AF, van Oppen MJH, Willis BL (2004) Flexibility in algal endosymbioses shapes growth in reef corals. Science 304: 1492–1494.
- 19. Cantin NE, van Oppen MJH, Willis BL, Mieog JC, Negri AP (2009) Juvenile corals can acquire more carbon from high-performance algal symbionts. Coral Reefs 28: 405–414.
- 20. Buddemeier RW, Fautin DG (1993) Coral bleaching as an adaptive mechanism. BioScience 43: 320–326.
- 21. Fautin DG, Buddemeier RW (2004) Adaptive bleaching: a general phenomenon. Hydrobiologia 530: 459–467.
- 22. Venn AA, Loram JE, Trapido-Rosenthal HG, Joyce DA, Douglas AE (2008) Importance of time and place: patterns in abundance of Symbiodinium clades A and B in the tropical sea anemone Condylactis gigantean. Biol Bull 215: 243–252.
- 23. Freeman CJ, Thacker RW (2011) Complex interactions between marine sponges and their symbiotic microbial communities. Limnol Oceanogr 56: 1577–1586.
- 24. Baird AH, Guest JR, Willis BL (2009) Systematic and biogeographical patterns in the reproductive biology of Scleractinian corals. Annu Rev Ecol Syst 40: 551–571.
- 25. Douglas AE (1998) Host benefit and the evolution of specialization in symbiosis. Heredity 81: 599–603.
- 26. Douglas AE (2010) The Symbiotic Habit. Princeton: Princeton University Press.
- 27. Bascompte J (2009) Mutualistic Networks. Front Ecol Envir 7: 429–436.
- 28. Vázquez DP, Blüthgen N, Cagnolo L, Chacoff NP (2009) Uniting pattern and process in plant-animal mutualistic networks: a review. Ann Bot 103: 1445–1457.
- 29. Aizen MA, Morales CL, Morales JM (2008) Invasive mutualists erode native pollination webs. PLoS Bio 6: e31.
- 30. Okuyama T, Holland JN (2008) Network structural properties mediate the stability of mutualistic communities. Ecol Lett 11: 208–216.
- 31. Kaiser-Bunbury CN, Memmott J, Müller CB (2009) Community structure of pollination webs of Mauritian heathland habitats. Persp Plant Ecol Evol and Syst 11: 241–254.
- 32. Ives AR, Carpenter SR (2009) Stability and diversity of ecosystems. Science 317: 58–62.
- 33. Blüthgen N, Menzel F, Hovestadt T, Fiala B, Blüthgen N (2007) Specialization, constraints, and conflicting interests in mutualistic networks. Curr Biol 17: 341–346.
- 34. Franklin EC, Stat M, Pochon X, Putnam HM, Gates RD (2012) GeoSymbio: a hybrid, cloud-based web application of global geospatial bioinformatics and ecoinformatics for Symbiodinium-host symbioses. Mol Ecol Res 12: 369–373 doi: https://doi.org/10.1111/j.1755-0998.2011.03081.x.
- 35. Palmer TM, Stanton ML, Young TP (2003) Competition and coexistence: exploring mechanisms that restrict and maintain diversity within mutualist guilds. Am Nat 162: S63–S79.
- 36. Putnam HM, Stat M, Pochon X, Gates RD (2012) Endosymbiotic flexibility associates with environmental sensitivity in scleractinian corals. Proc Royal Soc B. In press.
- 37. Loya Y, Sakai K, Nakano Y, Sambali H, van Woesik R (2001) Coral bleaching: the winners and the losers. Ecol Lett 4: 122–131.
- 38. van Woesik R, Sakai K, Ganase A, Loya Y (2011) Revisiting the winners and the losers a decade after coral bleaching. Mar Ecol Prog Ser 434: 67–76.
- 39. Marshall PA, Baird AH (2000) Bleaching of corals on the Great Barrier Reef: differential susceptibilities among taxa. Coral Reefs 19: 155–163.
- 40. LaJeunesse TC, Loh WKW, van Woesik R, Hoegh-Guldberg O, Schmidt GW, et al. (2003) Low symbiont diversity in southern GBR corals relative to those of the Caribbean. Limnol Oceanogr 48: 2046–2054.
- 41. Sampayo EM, Franceschinis L, Hoegh-Guldberg O, Dove S (2007) Niche partitioning of closely related symbiotic dinoflagellates. Mol Ecol 16: 3721–3733.
- 42. van Oppen MJH (2004) Mode of zooxanthellae transmission does not affect zooxanthellae diversity in acroporid corals. Mar Biol 144: 1–7.
- 43. Wicks LC, Sampayo E, Gardner JPA, Davy SK (2010) Local endemicity and high diversity characterize high-latitude coral-Symbiodinium partnerships. Coral Reefs 29: 989–1003.
- 44. Stat M, Loh WKW, Hoegh-Guldberg O, Carter DA (2008) Symbiont acquisition strategy drives host-symbiont associations in the southern Great Barrier Reef. Coral Reefs 27: 763–772.
- 45. Apprill AM, Gates RD (2007) Recognizing diversity in coral symbiotic dinoflagellate communities. Mol Ecol 16: 1127–1134.
- 46. Correa AMS, Baker AC (2009) Understanding diversity in coral-algal symbiosis: a cluster-based approach to interpreting fine-scale genetic variation in the genus Symbiodinium. Coral Reefs 28: 81–93.
- 47. Sampayo EM, Dove S, LaJeunesse TC (2009) Cohesive molecular genetic data delineate species diversity in the dinoflagellate genus Symbiodinium. Mol Ecol 18: 500–519.
- 48. Olesen J, Bascompte J, Elberling H, Jordano P (2008) Temporal dynamics in a pollination network. Ecology 89: 1573–1582.
- 49. Petanidou T, Kallimanis AS, Tzanopoulos J, Sgardelis SP, Pantis JD (2008) Long-term observation of a pollination network: fluctuation in species and interactions, relative invariance of network structure and implications for estimates of specialization. Ecol Lett 11: 564–575.
- 50. Bosch J, González AMM, Rodrigo A, Navarro D (2009) Plant-pollinator networks: adding the pollinator’s perspective. Ecol Lett 12: 409–419.
- 51. LaJeunesse TC (2001) Investigating the biodiversity, ecology, and phylogeny of endosymbiotic dinoflagellates in the genus Symbiodinium using the ITS region: in search of a “species” level marker. J Phycol 37: 866–880.
- 52. Spalding MD, Fox HE, Allen GR, Davidson N, Ferdaña ZA, et al. (2007) Marine ecoregions of the world: a bioregionalization of coastal and shelf areas. Bioscience 57: 573–583.
- 53. Newman MEJ (2010) Networks: an Introduction. Oxford: Oxford Publishing.
- 54. Oksanen J, Blanchet FG, Kindt R, Legendre P, O’Hara RB, et al. (2011) vegan: Community Ecology Package. R package version 1: 17–10.
- 55. Guimarães PR, Sazima C, dos Reis CF, Sazima I (2007) The nested structure of marine cleaning symbiosis: is it like flowers and bees? Biol Lett 3: 51–54.
- 56. Bascompte J, Jordano P, Melian CP, Olesen JM (2003) The nested assembly of plant-animal mutualistic networks. PNAS 100: 9383–9387.