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The PICALM Protein Plays a Key Role in Iron Homeostasis and Cell Proliferation

  • Paula B. Scotland,

    Affiliation Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Jessica L. Heath,

    Affiliation Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Amanda E. Conway,

    Affiliation Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Natasha B. Porter,

    Affiliation Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Michael B. Armstrong,

    Affiliation Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Jennifer A. Walker,

    Affiliation Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Mitchell L. Klebig,

    Affiliation Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, United States of America

  • Catherine P. Lavau,

    Affiliation Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America

  • Daniel S. Wechsler

    Affiliations Division of Pediatric Hematology-Oncology, Duke University Medical Center, Durham, North Carolina, United States of America, Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States of America

The PICALM Protein Plays a Key Role in Iron Homeostasis and Cell Proliferation

  • Paula B. Scotland, 
  • Jessica L. Heath, 
  • Amanda E. Conway, 
  • Natasha B. Porter, 
  • Michael B. Armstrong, 
  • Jennifer A. Walker, 
  • Mitchell L. Klebig, 
  • Catherine P. Lavau, 
  • Daniel S. Wechsler


The ubiquitously expressed phosphatidylinositol binding clathrin assembly (PICALM) protein associates with the plasma membrane, binds clathrin, and plays a role in clathrin-mediated endocytosis. Alterations of the human PICALM gene are present in aggressive hematopoietic malignancies, and genome-wide association studies have recently linked the PICALM locus to late-onset Alzheimer's disease. Inactivating and hypomorphic Picalm mutations in mice cause different degrees of severity of anemia, abnormal iron metabolism, growth retardation and shortened lifespan. To understand PICALM’s function, we studied the consequences of PICALM overexpression and characterized PICALM-deficient cells derived from mutant fit1 mice. Our results identify a role for PICALM in transferrin receptor (TfR) internalization and demonstrate that the C-terminal PICALM residues are critical for its association with clathrin and for the inhibitory effect of PICALM overexpression on TfR internalization. Murine embryonic fibroblasts (MEFs) that are deficient in PICALM display several characteristics of iron deficiency (increased surface TfR expression, decreased intracellular iron levels, and reduced cellular proliferation), all of which are rescued by retroviral PICALM expression. The proliferation defect of cells that lack PICALM results, at least in part, from insufficient iron uptake, since it can be corrected by iron supplementation. Moreover, PICALM-deficient cells are particularly sensitive to iron chelation. Taken together, these data reveal that PICALM plays a critical role in iron homeostasis, and offer new perspectives into the pathogenesis of PICALM-associated diseases.


The PICALM (phosphatidylinositol binding clathrin assembly protein) gene was originally identified as a translocation partner for AF10 (MLLT10) in the U937 leukemic cell line [1]. The ubiquitously expressed PICALM protein is involved in clathrin-mediated endocytosis [1][4]. PICALM localizes to developing clathrin-coated vesicles on the cytoplasmic side of the plasma membrane, associates with components of the endocytic machinery, and is required for endocytosis [2], [5]. Previous studies have demonstrated that overexpression of PICALM inhibits endocytosis of both the Transferrin (Tf) Receptor (TfR) [2] and Epidermal Growth Factor Receptor (EGFR) [2], [6]. Knockdown studies have shown that the absence of PICALM results in enlarged and abnormally shaped endocytic vesicles [4]. PICALM deficiency, as seen in mice homozygous or hemizygous for Picalmfit1 ENU (N-ethyl N-nitrosourea)-induced alleles [7], results in impaired hematopoiesis, anemia, abnormal iron metabolism, growth defects, and shortened life span [8][11]. In addition to its role in endocytosis, PICALM has been implicated in transcriptional regulation, and like several other proteins involved in the endocytosis machinery (EPS15, EPN1 and α-adaptin), transiently localizes to the nucleus [12], [13]. However, PICALM’s role in the nucleus is poorly understood.

The structural and functional domains of PICALM are similar to those of the neuronal specific protein AP180. These proteins are highly homologous but are differentially expressed. AP180 is only expressed in neurons, primarily in axons, while PICALM appears to be ubiquitous [14][18]. Both proteins have an amino-terminal ENTH (Epsin N-Terminal Homology) domain responsible for membrane association with PIP2 and a carboxy-terminal region that is believed to be involved in clathrin association [19][22]. Both proteins mediate clathrin assembly at the site of endocytic vesicle invagination and are thought to participate in controlling vesicle size [4].

The human PICALM gene (located at chromosomal band 11q14) has been implicated in several clinical disorders. In leukemias and lymphomas, PICALM has been identified as a translocation partner for the AF10 transcription factor gene (10p12) [1], [3], [23], and also for the Mixed Lineage Leukemia (MLL) histone methyltransferase gene (11q23) [24]. The resulting fusion genes, PICALM-AF10 and MLL-PICALM, have been found in aggressive hematologic malignancies [25][27]. While the chromosomal translocation that gives rise to MLL-PICALM is very rare [24], the PICALM-AF10 translocation is found in various hematopoietic malignancies and notably in 5–10% of T-cell acute lymphoblastic leukemias [3], [26]. Although the oncogenic properties of PICALM-AF10 have been characterized [3], [28], the specific role of PICALM in leukemogenesis has not been investigated. PICALM has also recently been implicated in late-onset Alzheimer's disease by genome-wide association studies [29]. It had been previously hypothesized that faulty endocytosis plays a role in the neuronal degeneration associated with Alzheimer's disease [30], and this notion is supported by the detection of specific PICALM SNPs in Alzheimer’s Disease patients [29], [31][33].

The present studies explore the physiological role of PICALM by determining the effects of PICALM overexpression in HEK293 cells and loss of PICALM expression in mouse embryonic fibroblasts (MEFs) derived from PICALM-deficient (Picalmfit1–5R/Picalmfit1–5R, hereafter referred to as PicalmNULL) mice [7]. Overexpression of PICALM impairs TfR endocytosis, and mutagenesis of the PICALM cDNA has allowed us to identify the domains that are critical for this function. Studies of PICALM-deficient cells corroborated the essential role of PICALM in endocytosis and also revealed a previously unappreciated role in iron homeostasis. The rate of TfR endocytosis, the expression of total TfR protein and mRNA, and intracellular iron levels were all affected in PICALM-deficient cells. These cells also display a proliferation defect compared with their wild type (WT) counterparts. Rescue of PICALM expression by retroviral transduction restores endocytosis, TfR expression, intracellular iron levels, and proliferation in these cells. Furthermore, the proliferation defect of PICALM-deficient cells appears to be due to iron deficiency, as iron supplementation restores their proliferation to normal levels. Finally, PICALM-deficient cells are more sensitive to the growth inhibitory effect of iron chelation, further illustrating the importance of PICALM in intracellular iron homeostasis. Our findings establish that PICALM is required for endocytosis of the TfR, and that this in turn impacts the ability of cells to import iron. This study is also the first to demonstrate a role for PICALM in cellular proliferation. These observations raise the possibility that perturbed iron metabolism may play a role in PICALM-associated disease states.


A PICALM Carboxy-terminal Domain is Essential for Inhibition of Endocytosis and Mediates Clathrin Binding

Previous studies have demonstrated that overexpression of full-length PICALM impairs endocytosis [2], likely by a dominant negative mechanism whereby higher levels of PICALM sequester components of the endocytic machinery. To determine which specific PICALM regions are required in endocytosis, we prepared GFP-labeled PICALM truncation mutants missing the amino- and carboxy-terminal domains (Figure 1A). As expected, the rate of TfR internalization in transfected HEK293 cells overexpressing GFP-PICALM was reduced by >90% in comparison with cells transfected with empty GFP vector (Figure 1B, GFP vs. PICALM:1–652). Although the amino-terminal 255 amino acid residues of PICALM (PICALM:256–652) alone are not necessary for this inhibitory effect, a deletion mutant truncated at amino acid 583 (PICALM:256–583) resulted in a significant loss of the ability to inhibit endocytosis (Figure 1B and Figure S1A). These results indicate that the carboxy-terminal 69 amino acid (aa) residues of PICALM play an important role in clathrin-mediated endocytosis (CME).

Figure 1. The carboxy-terminal 69 amino acids of PICALM are essential for binding clathrin and interfering with TfR endocytosis.

(A) Schematic representation of amino-terminal-tagged GFP-PICALM fusion proteins. (B) TfR internalization in HEK293 cells transiently transfected with empty vector (control), full length PICALM (PICALM: 1–652), amino-terminal truncated PICALM (PICALM: 256–652), or a PICALM mutant missing both the amino and carboxy-terminal regions (PICALM: 256–583). The relative rate of endocytosis was calculated as described in Materials and Methods. Nexp  = 11. *p<0.001 compared with control. (C) Western blot of proteins co-immunoprecipitated (IP) with anti-GFP antibody and immunoblotted (IB) with anti-clathrin heavy chain (CHC) antibody (upper panel). Cell lysates (input) were also immunoblotted with anti-CHC and anti-PICALM antibodies (lower panels). The molecular weights of GFP-PICALM, GFP-PICALM:256–652 and GFP-PICALM:256–583 are 97kD, 70kD and 62 kD, respectively.

To determine whether an interaction with clathrin correlates with PICALM-mediated inhibition of endocytosis, full-length and truncated GFP-PICALM proteins were immunoprecipitated from transfected cells (anti-GFP antibody) and probed with an anti-clathrin heavy chain antibody. The same GFP-PICALM proteins that inhibit TfR internalization, full-length (aa 1–652) and amino-terminally truncated PICALM (aa 256–652), also bind to and immunoprecipitate clathrin (Figure 1B & C). In contrast, a mutant protein lacking both the C-terminal 69 amino acids and the N-terminal 255 residues of PICALM (aa 256–583), which failed to inhibit TfR internalization, does not interact with clathrin (Figure 1B & C, and Figure S1A, lower blot). These observations suggest that the interaction of PICALM with clathrin via its C-terminal 69 aa residues could be a mechanism for its inhibitory effect on CME when it is overexpressed in HEK293 cells.

To identify the specific amino acids within the PICALM carboxy-terminal region that are responsible for CME inhibition and clathrin binding, a series of deletion and point mutations were analyzed. These PICALM variants were expressed in HEK293 cells, and the rate of TfR internalization was measured. Analysis of the deletion mutants revealed that inhibition of TfR internalization increased progressively as more of the PICALM carboxy-terminal region was present (Figure S1A top). In addition, only truncation mutants that inhibited TfR internalization also co-immunoprecipitated with clathrin heavy chain (Figure S1A bottom), further supporting the importance of clathrin binding in PICALM’s role in endocytosis. PICALM overexpression may act through a dominant negative mechanism by sequestering clathrin. To identify the critical residues of PICALM that are required for inhibition of CME, site-directed mutagenesis spanning amino acids 583 to 652 was performed using NAAIRS mutagenesis to minimize changes in protein structure [34]. NAAIRS mutagenesis involves substituting nucleotides encoding six sequential amino acids of interest with those encoding amino acids asparagiNe-Alanine-Alanine-Isoleucine-aRginine-Serine (NAAIRS). This sequence is capable of forming either an alpha helix or a beta-sheet, and thus is more suitable for maintaining the secondary and tertiary structure of the protein while testing the function of the specific amino acids of interest. Each of eleven distinct NAAIRS mutants (Figure S1C) inhibited TfR internalization, much like full-length, normal PICALM (Figure S1B top). In addition, each of these mutants also co-immunoprecipitated with clathrin, albeit to varying degrees (Figure S1B bottom). In fact, one double mutant, NPF-IGYGIP, chosen for further study because it bound significantly less clathrin, was still able to inhibit endocytosis. Because extensive point mutagenesis of the PICALM gene within the last carboxy-terminal 69 amino acids did not identify a single specific site responsible for clathrin association and inhibition of TfR internalization, it is likely that there are multiple cooperating motifs within this region that ensure association with clathrin and possibly other components of the clathrin-mediated endocytic machinery.

PICALM-deficient MEFs Display Increased Surface TfR and Reduced Endocytosis

Overexpression studies in HEK293 cells showed that excess levels of PICALM perturb TfR internalization, consistent with previous studies [2]. Interestingly, loss of PICALM function has also been reported to impair CME and receptor internalization [4]. To analyze the effects of PICALM deficiency and the requirement for PICALM in TfR endocytosis, we isolated embryonic fibroblasts from either normal or PICALM-deficient (Picalmfit1–5R/Picalmfit1–5R) mice. The ENU-induced Picalmfit1–5R allele has a point mutation in the splice donor site immediately downstream of Picalm exon 4 that causes aberrant splicing and encodes a severely truncated PICALM protein that includes only the first 116 amino acids [7]. Because the mutant protein lacks the putative clathrin-binding domain and almost all other known domains, the Picalmfit1–5R allele is considered to be a null [7]. Heterozygous mice were bred, and Picalm+/+ or Picalmfit15R/fit15R (hereafter referred to as WT or PicalmNULL, respectively) mouse embryonic fibroblasts (MEF) were isolated from E14 embryos. The MEFs were either grown as primary cells or immortalized with SV40 T/t antigen. Seven different immortalized lines (three WT lines, four PicalmNULL lines) were generated from three different pregnancies (Figure S2A). In all experiments, PicalmNULL cells were compared with WT cells derived from littermate embryos. Flow cytometry analysis of both primary and immortalized MEFs revealed that PICALM-deficiency was accompanied by a dramatic increase in surface TfR expression (2–5 fold increase compared to WT cells, Figure 2A and 2C). We used a flow cytometry-based assay to measure endocytosis of TfR over time and because of the considerable difference in expression levels between PICALMNULL and WT cells, TfR uptake was normalized to the total amount of TfR at the cell surface. At 3 minutes, 20±1 or 23±3 (percent±SE) of TfR was internalized in non-immortalized or immortalized PICALM-deficient MEFs, respectively, while 33±3 or 37±5% of TfR was internalized in their WT counterparts (Figure 2B and 2D). Remarkably, despite the reduced efficiency of TfR internalization in PICALM-deficient MEFs, the increased cell surface levels of the receptor resulted in a higher net amount of internalized TfR in PICALM deficient cells (4,800±200 and 24,300±400, MFI±SE, non-immortalized or immortalized MEFs, respectively) compared with WT control MEFs (2,750±900 and 15,300±3,400) (raw data before normalization, not shown).

Figure 2. Absence of PICALM results in a decreased rate of internalization and increased cell surface expression of TfR in MEFs.

The level of cell surface TfR expression normalized to levels in WT cells (A,C,E) and the rate of TfR internalization (B,D,F) was measured in non-immortalized MEFs (A,B), immortalized MEFs (C,D), or PICALM shRNA knockdown WT (line 7T) MEFs (E,F). (A) Surface TfR expression in non-immortalized WT and PicalmNULL MEFs. Nexp  = 4. *p<0.02 compared with WT. (B) TfR internalization at three different time points in independently derived non-immortalized WT (lines 20, 33) and PicalmNULL (lines 24, 29, 31). Nexp  = 2. *p<0.04 compared with WT. (C) Surface TfR expression in immortalized WT and PicalmNULL MEFs. Nexp  = 4. *p<0.04 compared with WT. (D) TfR internalization in independently derived immortalized WT (lines 20T, 33T) and PicalmNULL (lines 24T, 29T, 31T). Nexp  = 7. *p<0.01 compared with WT. (E) Surface TfR expression in shRNA transduced WT MEFs. Nexp  = 4. *p<0.02 compared with WT. (F) TfR internalization in immortalized WT (line 7T) transduced with two separate PICALM shRNA retroviruses (shRNA4, shRNA5) or a control shRNA retrovirus. Nexp  = 3. *p<0.01 compared with WT. In all cases, error bars denote standard error. Two-tailed Student’s t-test was used to compare means for TfR internalization (A,C,E).

To confirm the role of PICALM in TfR endocytosis, we stably knocked down its expression using shRNAs into immortalized WT MEFs and also HEK293 cells. Two shRNAs (shRNA4 and shRNA5) that induced greater than 60% reduction of PICALM protein expression in MEFs were compared with a control shRNA (Figure S2B). Consistent with the phenotype of PicalmNULL MEFs, shRNA knockdown of Picalm expression was accompanied by an increase in surface TfR expression (Figure 2E) and a significant reduction of TfR internalization efficiency (Figure 2F). Similarly, knockdown of Picalm in HEK293 cells (Figure S2C) resulted in increased surface TfR (Figure S2D) and reduced TfR internalization efficiency (Figure S2E), confirming the observations in MEFs.

In order to determine whether the increase in surface TfR was caused by changes in TfR expression levels or mislocalization of the protein, total cell TfR protein was quantified by infrared fluorescence analysis of immunoblots. PicalmNULL MEFs showed an approximately two-fold TfR increase compared with WT controls (Figure 3A and 3B). Transcript quantification by RT-PCR analysis similarly showed higher levels of TfR mRNA in PicalmNULL cells (Figure 3C). The increased expression of TfR in PICALM-deficient cells likely reflects a state of relative iron deficiency, characterized by increased TfR mRNA stability and protein translation, and is consistent with the iron deficiency phenotype originally described in the fit1 mutant mice [7], [8].

Figure 3. PICALM-deficient cells display increased total TfR protein and mRNA.

(A) Immunoblot of total TfR protein, PICALM, and β-actin from PicalmNULL (Null), WT, PicalmNULL control MEFs infected with empty vector (Null + control), or PicalmNULL MEFs rescued with PICALM (Null + PICALM). PICALM cDNA encodes for the larger isoform. (B) Quantitation of TfR shown in panel A immunoblot normalized to β-actin, with values shown relative to TfR level in WT MEFs. Nexp  = 3. *p<0.04. (C) RT-PCR quantitation of TfR mRNA in PICALM-deficient (Null) MEFs, WT MEFs, PicalmNULL MEFs transduced with empty vector (Null + Control) or rescued with PICALM (Null + PICALM). Results are normalized to levels in Null MEFs or Null + Control cells. Nexp  = 3. *p<0.04 compared with WT cells.

The PICALM Carboxy-terminal Region is Essential for Rescue of the PICALM-deficient Phenotype

To confirm that the phenotype of PicalmNULL MEFs results from the absence of PICALM, we used retroviral transduction to restore PICALM expression. Immortalized PICALM-deficient MEF lines were stably infected with either an empty retroviral vector or a vector encoding full length PICALM. As shown in Figure 4A, expression of full-length PICALM rescued the efficiency of TfR internalization. Expression of PICALM also lowered cell surface expression of TfR protein in the different MEF lines (Figure 4B). Furthermore, both total TfR protein and mRNA levels decreased upon PICALM transduction of PicalmNULL cells (Figures 3B and 3D). The ability of PICALM to rescue the efficiency of TfR endocytosis and to reduce its surface expression were confirmed in three additional independently derived PicalmNULL MEF lines (Figures S3A, S3B). These findings demonstrate that PICALM deficiency is responsible for defective TfR endocytosis and altered TfR expression in murine cells.

Figure 4. PICALM expression rescues the PICALM-deficient phenotype, and the PICALM carboxy-terminal domain plays an essential role.

(A) Representative experiment showing rate of TfR internalization in PicalmNULL MEFs (line 31T) stably infected with PICALM or empty vector (control) compared with WT (line 33T) MEFs. (B) Surface TfR expression in WT MEFs (7T, 20T, 33T) or PicalmNULL MEFs (lines 3T, 24T, 29T, 31T) that were uninfected, stably infected with empty vector (control) or PICALM. Surface TfR levels were normalized to levels in control PicalmNULL MEFs. Nexp  = 8. *p<0.001 compared with control. TfR endocytosis and surface TfR levels measured in three independent PicalmNULL lines is shown in Figure S3. (C) Deletion mutagenesis of the PICALM carboxy-terminal domain (constructs depicted in left panel) demonstrates that the ability to rescue surface TfR expression is dependent on the PICALM carboxy-terminal domain. Nexp  = 3. *p<0.02 compared with PICALM-17a. (D) Co-immunoprecipitation of lysates from HEK293 cells transiently transfected with full length PICALM (PICALM: 1–652), carboxy-terminal truncated PICALM (PICALM: 1–583), or a PICALM mutant missing both the amino and carboxy-terminal regions (PICALM: 256–583). Extracts were immunoprecipitated (IP) with anti-GFP antibody and immunoblotted (IB) with anti-clathrin heavy chain (CHC) antibody (upper panel). Cell lysates (input) were also immunoblotted with anti-PICALM antibodies (lower panel).

We used the PICALM rescue assay of PicalmNULL cells to determine the regions of the protein required to lower surface expression of TfR. To this end, a series of PICALM deletion/truncation mutants was stably expressed in the 3T PicalmNULL line, and surface TfR levels were analyzed by flow cytometry. While full length PICALM reduced the levels of surface TfR more than two-fold, constructs lacking the carboxy-terminal 122 amino acids (1–485, 1–520, 1–530), were significantly less potent (Figure 4C). The progressive inclusion of residues 540 to 583 (1–540, 1–550, 1–583) increased the ability to rescue the PICALM-deficient phenotype. In particular, the 1–583 truncation mutant was able to rescue the surface TfR overexpression phenotype to the same extent as normal PICALM (Figure 4C), even though it lacks the C-terminal domain important for clathrin interaction (Figure 1C and 4D). These results indicate that the N-terminal 583 aa of PICALM are critical for rescuing surface TfR overexpression, and that the 530–583 domain is of particular importance for this rescue.

Little is known about the functional significance of alternatively spliced PICALM variants. Given the carboxy-terminal position of the eight amino acids (NGMHFPQY, at position 593) encoded by exon 17a, we tested the impact of their inclusion on PICALM's ability to restore surface TfR levels in PicalmNULL cells. The presence of this exon did not affect PICALM’s activity (Figure 4C, PICALM-17a vs. PICALM+17a). Likewise, the inclusion of alternatively spliced exon 13a did not alter the ability of PICALM to rescue TfR expression (data not shown). The functional role of these alternatively spliced variants remains to be discovered.

PicalmNULL Cells are Iron Deficient

Tf plays an essential role in importing iron into cells by binding to its receptor, TfR, on the cell surface, followed by clathrin-mediated endocytosis. To determine whether iron homeostasis is perturbed in PicalmNULL cells, we quantified levels of intracellular iron. We first measured the labile iron pool using the iron chelating reagent Phen Green SK (PGSK) [35]. PGSK is a membrane permeable dye, the fluorescence of which is quenched in the presence of labile iron (Fe2+ or Fe3+). Thus, the fluorescence of PGSK is inversely proportional to the amount of intracellular labile iron. In the 3T, 24T and 29T PicalmNULL lines, the PGSK fluorescence was higher than in the WT counterparts, indicating that the labile iron pool of PicalmNULL cells was reduced (Figure 5A). To limit the intrinsic variability between independent cell lines, we compared PGSK fluorescence in PicalmNULL lines with or without PICALM rescue. Retroviral transduction of PICALM in three separate PicalmNULL cell lines (3 T, 24 T, and 29 T) significantly increased PGSK quenching (Figure 5B), indicating that PICALM expression restored intracellular labile iron pool levels. As an alternative approach, we used mass spectroscopy to measure whole cell iron content. Total iron levels were measured in the PicalmNULL line 3 T transduced with either control vector or PICALM (Figure 5C). Although the difference did not reach statistical significance, PICALM rescued cells contained more iron than vector controls, further suggesting a role of PICALM in increasing cellular iron.

Figure 5. PICALM-deficient MEFs exhibit decreased intracellular iron compared with WT or PICALM rescued MEFs.

(A) Chelation of Phen Green SK (the fluorescence of which inversely correlates with intracellular iron levels) was measured in WT (lines 7T, 20T) or PICALM-deficient (PicalmNULL – lines 3T, 24T, 29T) MEFs. Values were normalized to those in WT MEFs. Nexp  = 3. *p<0.04 compared with WT. (B) Chelation of Phen Green SK in PicalmNULL cells that were uninfected or rescued with PICALM. Values are normalized to levels in PICALM-rescued cells. Nexp  = 3. *p<0.04 compared with PICALM-rescued cells. (C) Total intracellular iron levels in PicalmNULL (line 3T) and PICALM rescued MEFs, normalized to levels in PicalmNULL cells. Nexp  = 3. MFI: mean fluorescence intensity.

Insufficient Intracellular Iron Limits the Proliferation of PicalmNULL Cells

While establishing MEF cultures from PicalmNULL and WT embryos, we observed that PICALM-deficient MEFs consistently took longer to reach confluence. To determine whether PICALM influences the rate of cell proliferation, we plated non-immortalized PicalmNULL and WT MEFs at fixed densities and compared their proliferation rates over 5 days (Figure 6A, 6C). WT MEFs proliferated at considerably higher rates than PicalmNULL cells (the cell expansion at day 5 was on average 2.5-fold higher for WT compared with PicalmNULL MEFs). To determine whether the reduced proliferation of PicalmNULL MEFs was attributable to lower levels of intracellular iron, proliferation assays were repeated in the presence of supplemental iron in the form of ferric ammonium citrate (FAC). FAC is a low-molecular-weight form of iron that can penetrate into cells by bypassing the transferrin endocytic pathway [36]. When FAC (50 µM) was added to culture medium, the proliferation rate of PICALM-deficient MEFs was restored to that of WT cells (Figure 6B, 6D). This demonstrates that the growth of PICALM-deficient cells is limited by iron availability and further supports the importance of PICALM in ensuring optimal iron import into cells.

Figure 6. PICALM-deficient MEFs proliferate more rapidly in the presence of iron supplementation and are more sensitive to iron chelation.

(A,B) Representative proliferation curves of non-immortalized PicalmNULL and WT MEFs without (A) and with (B) 50 µM ferric ammonium citrate (FAC). FAC restores proliferation in PicalmNULL cells to levels in WT cells. (C,D) Mean cell numbers (+/− standard error) of non-immortalized PicalmNULL and WT MEFs grown for 5 days without (C) and with (D) 50 µM FAC. Nexp  = 3. *p<0.04 compared with WT cells. (E,F) Representative proliferation curves of immortalized PicalmNULL MEFs transduced with empty vector (control) or PICALM grown for 4 days in the absence (E) or presence (F) of 2.5 µM deferoxamine (DFO). (G) Mean cell number of PicalmNULL MEFs untransduced (Null 3T) or transduced with empty vector (control) or PICALM after 4 days of culture in the presence of 2.5 µM DFO treatment relative to number of untreated cells. Nexp  = 4. *p<0.003 compared with PICALM rescued cells.

Finally, we examined whether PicalmNULL MEFs are more sensitive to a reduction in extracellular iron concentration. To this end, the proliferation of immortalized MEFs was measured in the presence of the iron-chelating drug deferoxamine (DFO). While immortalized PicalmNULL MEFs displayed a similar high rate of proliferation in regular culture media regardless of the presence of PICALM (Figure 6E), PicalmNULL cells transduced with empty vector were more sensitive to the growth inhibitory effect of DFO than PicalmNULL cells transduced with PICALM (Figure 6F, 6G). These findings indicate that when iron concentration is limiting, the presence of PICALM is required for optimal cell proliferation.


Internalization of transferrin-bound iron via the transferrin receptor (TfR) is the primary mechanism of cellular iron import, and is dependent on clathrin-mediated endocytosis (CME). A disruption of CME would therefore be expected to reduce intracellular iron levels. PICALM, a homologue of the previously identified endocytic protein AP180 [1], is involved in promoting clathrin coat assembly in the early stages of vesicle budding from the membrane [2], [6], [37]. Overexpression of PICALM impairs endocytosis of both the TfR and the Epidermal Growth Factor Receptor (EGFR), likely via dominant negative interference [2], [6]. Previous studies using siRNAs to downregulate PICALM expression in HeLa and HEK293 cells concluded that PICALM is not required for TfR endocytosis [4], [6], [38], [39]. However, depletion of PICALM impairs endocytosis of EGFR and VAMP2 (synaptobrevin 2) [6], [38], in addition to causing defects in the size and shape of clathrin-coated structures [4], and affecting the maturation of clathrin-coated pits [5]. Furthermore, a role for PICALM in sorting of R-SNAREs (VAMP2, VAMP3, VAMP8) into endocytic vesicles has recently been described [39].

An indication of a critical role for PICALM in iron metabolism came from studies of the fit1 mutant mouse. fit1 mice were initially generated using N-ethyl-N-nitrosourea (ENU) mutagenesis, and were subsequently shown to have a nonsense point mutation in the Picalm gene resulting in a truncated and nonfunctional PICALM protein [7]. Mice homozygous or hemizygous for Picalmfit1 mutations display dramatic growth retardation compared with their normal littermates [8], [40]. These mice also show a distinct hematologic phenotype: they are anemic and have hypochromic red blood cells. Serum and tissue iron levels are also significantly lower in all but liver cells, which show a dramatic increase in iron stores [10]. Here, we characterize TfR endocytosis and proliferation properties of Picalm-deficient MEFs derived from fit1 mice. Our studies suggest a defect in iron homeostasis as a contributory mechanism for the phenotype of these mice.

Our results demonstrate that internalization of TfR is impaired in PicalmNULL MEFs and shRNA treated MEFs. Importantly, a recent report [41] also found that Tf endocytosis was impaired in erythroid cells and MEFs derived from PICALM-deficient mice, corroborating our observations. It is possible that the discrepancy of our results and those of Suzuki et al. [41] with previous reports suggesting that PICALM knockdown does not affect TfR endocytosis [4], [6], [38], [39] relates to the use of different methodologies to assess endocytosis, such as length and temperature of incubations and inclusion of acid washes; notably, previous reports used either fluorescence microscopy [38] or isotope labeled Tf [6], [39] to quantitate TfR internalization, whereas we and Suzuki et al. [41] used flow cytometry. We also measured the fraction of TfR internalized relative to the total surface TfR expression, while other reports measured the absolute amount of TfR internalized. This is of importance because the relative reduction in TfR internalization seen in PICALM-deficient cells could be due to a saturation of the cellular endocytic machinery resulting from the overexpression of TfR secondary to iron deficiency. Alternatively, the complete absence of functional PICALM in fit1−/− MEFs might be expected to have a greater impact on TfR endocytosis than RNA interference-based knockdown approaches, which do not necessarily result in a complete absence of functional PICALM. To address this possibility, we used shRNA to knockdown PICALM expression in wild type MEFs and in HEK293 cells, and found that this also results in impaired TfR endocytosis (Figure 2E, 2F, S2D, S2E). Of note, we have also observed impaired TfR endocytosis in PicalmNULL murine hematopoietic cells (data not shown) suggesting that the requirement of PICALM for TfR endocytosis is not cell type specific. Thus, it seems most likely that the use of different assays for TfR internalization may be the reason for the the apparent discrepant results about the role of PICALM in TfR endocytosis that have been reported in the literature.

Consistent with a defect in TfR endocytosis, PICALM-deficient MEFs display features of iron deficiency, including increased TfR expression at the protein and transcript levels, reduced concentrations of intracellular iron, and a defect in proliferation that can be restored by iron supplementation. The expression of TfR is regulated post-transcriptionally by iron-responsive elements in the 3′ UTR that stabilize TfR mRNA when intracellular iron levels decrease [42]. Consistent with an iron deficient state, PicalmNULL MEFs display an approximately two-fold increase in both TfR mRNA and protein levels compared to WT cells (Figure 3). The defect in TfR internalization also likely contributes to the more pronounced two- to five-fold increase in cell surface TfR seen by flow cytometry in the PicalmNULL MEFs (Figure 2D). Although the TfR internalization defect may play a causal role in the iron deficiency of PicalmNULL cells, the compensatory increase in TfR expression appears to enable the cells to internalize iron in sufficient amounts. This raises the possibility that PICALM is required for other steps that mediate the release of iron from endocytic vesicles; this could relate to PICALM’s role in endocytosis of R-SNAREs that can be expected to perturb trafficking of various proteins through the endocytic pathway [39].

One of the strengths of the PICALM-deficient MEF system is the ability to rescue the mutant phenotype (increased surface TfR expression, impaired TfR endocytosis, decreased intracellular iron, reduced proliferation rate) through restoration of PICALM expression by retroviral transduction. Previous studies have demonstrated that the carboxy-terminal third of the PICALM protein (aa 414–652) binds the clathrin heavy chain (CHC) [2]. Our overexpression studies in HEK293 cells (which have normal PICALM function) further narrowed the region involved in CHC binding to a 69 amino acid domain at the PICALM C-terminus (aa 583–652). However, in Picalm-deficient MEFs, we found that while the PICALM aa 1–530 construct is unable to rescue TfR expression, PICALM 1–583 is sufficient to achieve the same level of TfR surface expression as is achieved with normal PICALM (Figure 4C). This suggests that the PICALM aa 530–583 domain is of particular importance for rescue, and that specific clathrin binding by the C-terminal 69 aa is not required. It is possible that PICALM interacts with clathrin through aa 530–583 in the context of murine cells; this remains to be demonstrated formally. In summary, the effect of PICALM construct overexpression in Picalm-deficient MEFs (Figure 4C) appears to be different from that in PICALM-sufficient HEK293 cells (Figure 1B). We have shown that approximately 120 carboxy-terminal PICALM residues play a role in PICALM-dependent CME: aa 583–652 are required for inhibiton of TfR internalization in HEK293 cells, and aa 530–583 are essential for rescue of the iron-deficient phenotype in PicalmNULL MEFs. It is likely that different mechanisms are used under these specific circumstances, with a dominant negative effect at play in HEK293 cells, and interactions with other components of the endocytic machinery in PicalmNULL MEFs.

While the PICALM C-terminal region confers the ability to bind clathrin, no characteristic structural motif has been identified within it. Surprisingly, using scanning site-specific NAAIRS mutagenesis, we were unable to further narrow down a specific clathrin-binding domain. Immunoprecipitation studies revealed that NAAIRS mutations blanketing the entire carboxy-terminal 69 aa domain, singly and in combination, did not abolish clathrin binding. This suggests that PICALM must interact with clathrin through multiple binding sites within this domain, reminiscent of the repetitive clathrin assembly motifs present in the AP180 protein [22]. Taken together, we conclude that the iron deficiency phenotype of fit1 mice is attributable to PICALM deficiency, and we have identified the carboxy-terminus of PICALM as the essential region.

The effect of PICALM on iron import has important biological consequences, since reduced levels of intracellular iron appear to limit the rate of cellular proliferation. This was revealed by observing that the slower growth of non-immortalized PicalmNULL MEFs could be corrected by supplementing the cells with iron. The proliferation defect observed in the PicalmNULL MEFs is consistent with the smaller size of fit1 mutant mice [8]. It is curious that upon immortalization by SV40 T/t antigen, PicalmNULL MEFs did not show the same growth disadvantage (Figure 6E), suggesting that oncogenic transformation may involve alterations in metabolic pathways that lessen the need for iron or trigger other compensatory elements. However, when immortalized cells were challenged with the iron-chelating agent DFO, PICALM-deficient cells demonstrated higher sensitivity, indicating that these cells are still limited in their ability to internalize iron.

The observation that normal cell proliferation appears to require PICALM is intriguing given the involvement of PICALM in chromosomal translocations in hematopoietic malignancies. It is conceivable that PICALM fusion proteins may enhance cell proliferation by boosting iron uptake. In the case of PICALM-AF10 translocations, many of the resulting fusion proteins contain almost the entire PICALM protein (except for the last four carboxy-terminal amino acids [25], [43]); the PICALM-AF10 fusion protein may still function in endocytosis and enhance iron uptake. Alternatively, PICALM rearrangements may confer a growth advantage by impairing endocytosis, with consequent upregulation of the surface expression of growth factor receptors, leading to persistent signaling and enhanced proliferation [44][47]. Leukemias with PICALM translocations also have PICALM haploinsufficiency, which could manifest as a relative deficiency of PICALM protein expression. The resulting defective endocytosis could render PICALM-deficient leukemia cells relatively iron deficient, and therefore more sensitive to reduced levels of extracellular iron. This raises the possibility that iron chelation could be of benefit in the treatment of patients with PICALM haploinsufficient leukemias. Of note, iron chelators are being studied as novel therapeutic agents in many malignancies, including leukemias [48].

Finally, the role of PICALM in iron homeostasis may also be relevant to the pathogenesis of Alzheimer's Disease (AD). It has long been recognized that brain iron content is altered in AD [49][52]: accumulation of excess brain iron is associated with AD, and “iron mismanagement” has been postulated to contribute to AD development [50]. Recently, multiple studies have shown the genetic association of PICALM polymorphisms with late onset AD [29], [33], [53], [54], although the mechanism by which PICALM contributes to the disease is unknown [55]. In particular, it is uncertain whether PICALM polymorphisms are associated with increased or decreased PICALM activity. Our observation that modulation of PICALM expression affects intracellular iron levels suggests a possible mechanism by which PICALM gene alterations could play a role in AD pathogenesis, and further investigation of this pathway may be warranted.

In summary, we have demonstrated that PICALM expression levels affect TfR endocytosis and alter iron homeostasis. PICALM-deficient cells proliferate more slowly than their WT counterparts, and PICALM re-expression in these cells restores endocytosis, intracellular iron levels and proliferation. PICALM-deficient cells are affected by manipulation of extracellular iron levels and are particularly sensitive to iron chelation. These observations raise the possibility that perturbed iron metabolism may play a role in PICALM-associated disease states.

Materials and Methods

Ethics Statement

All in vivo and euthanasia procedures in this study were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The animal studies described here have been approved by the Duke University Institutional Animal Care & Use Committee (IACUC) (Protocol# A029-10-02). All efforts were made to minimize animal suffering.

Cell Culture

HEK293 (ATCC) and retroviral packaging Plat-E cells [56] (a generous gift of T. Kitamura), were maintained in DMEM supplemented with 10% fetal bovine serum, penicillin, and streptomycin (Invitrogen, Carlsbad, CA, USA). Mouse embryonic fibroblasts (MEFs) were maintained in the same basic medium, supplemented with non-essential amino acids, glutamine, Fungizone® and gentamicin (Invitrogen). HEK293 and Plat-E cells were transfected by calcium chloride transfection [57]. HEK293 cells stably transfected with shRNAs encoding pRSMX_PG vectors were selected using puromycin (Sigma). Analysis of endocytosis by flow cytometry was done at least two days after transfection. MEFs were infected by co-culture with filtered (0.2 µm filter) Plat-E supernatant, in the presence of Polybrene® (2 µg/mL). Transfection/infection efficiencies were verified by analysis of GFP percentage by flow cytometry (Accuri C6, Ann Arbor, MI, USA).

DNA Constructs, Vectors and Mutagenesis

A human PICALM cDNA lacking both exon 17a [NGMHFPQY] and a portion of exon 13 [DPFSATV] was subcloned into the GFPN1 vector (Stratagene, La Jolla, CA, USA), downstream of the GFP gene. Full length PICALM and all point mutants included a hemagglutinin (HA) carboxy-terminal tag followed by a stop codon. PICALM truncation mutants were generated by PCR using oligonucleotides to introduce a 3′ TAA stop codon without a HA tag. All infections into MEFs for rescue assays were performed using human PICALM cDNA subcloned into the bicistronic MSCV-IRES-eGFP (MIE) retroviral vector [58]. Deletion PICALM constructs expressed in the MIE vector were generated by standard PCR protocols using oligonucleotides that hybridized to the appropriate regions of PICALM with flanking XhoI (5′ end) or BamHI (3′ end) restriction sites and a TAA stop codon at the 3′ end immediately after the indicated amino acid. All point mutations within the PICALM gene were made using the Stratagene Site Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA). The NPF mutant changed the indicated codons to encode AAA (5′-GCGGCCGCT-3′), incorporating a NotI site within the mutation site. NAAIRS mutagenesis [34], [59] was performed by replacing consecutive stretches of six amino acids with sequence encoding the amino acids NAAIRS (5′-AATGCAGCAATAAGATCT-3′), as well as a BglII site to facilitate screening. Primary MEFs were immortalized with the SV40 DNA tumor virus early region T/t antigen in the pBABE vector (courtesy of Dr. Corinne Linardic, Duke University).

Picalm shRNA were expressed using the GFP-encoding pRSMX_PG retroviral vector [60] (kindly provided by L. Staudt, NIH). The control shRNA directed against luciferase (5′-GTGGATTTCGAGTCGTCTTTAAT-3′), Picalm shRNA4 (5′-GCCTTAATGTTGACTTTGAAT-3′), and Picalm shRNA5 (5′-GCAGCATACAATGAAGGAATT-3′) were cloned into HindIII and BglII sites.

Antibodies and Reagents

The following reagents were used: PE-conjugated-mouse-anti-CD71 (eBioscience, San Diego, CA, USA), Alexa Fluor® 633 conjugated transferrin (Invitrogen), goat-anti-PICALM antibody (C-18, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), mouse-anti-clathrin heavy chain (TD.1, Sigma-Aldrich, St. Louis, MO, USA), rabbit anti- PICALM antibody (Sigma), mouse-anti-actin antibody (AC-40, Sigma), rabbit-anti-actin antibody (Sigma), mouse-anti TfR antibody (Invitrogen), Phen Green SK diacetate (Invitrogen), deferoxamine mesylate salt (DFO, Sigma), ammonium iron (III) citrate (FAC, Sigma), and rabbit-anti-GFP antibody (Stratagene).

Cell Surface Labeling of Transferrin Receptor and Endocytosis of Transferrin/transferrin Receptor

HEK293 cells or MEFs growing exponentially were serum starved for 2 hours before being trypsinized briefly with 0.05% trypsin/EDTA (Invitrogen) until only 30–40% of the cells were lifting off the plate to minimize cleaving of surface TfR. Trypsinization was stopped with serum-containing medium and cells were washed with cold PBS and resuspended in 1% bovine serum albumin. Samples to be analyzed with anti-CD71 antibody were blocked using rat IgG (1 µg/mL). Alexa Fluor® 633-conjugated transferrin (50 µg/mL) or PE-conjugated anti-CD71 antibody was added to the cells, and the samples were incubated on ice for 1 hour in the dark. Cells were washed twice with cold PBS and analyzed by flow cytometry (C6, Accuri) to measure surface TfR levels, or further processed to measure rate of TfR endocytosis. For endocytosis assays, each sample was divided into multiple tubes that were incubated in a 25°C water bath for the indicated times. Endocytosis assays were conducted at 25°C instead of 37°C to slow down the process in order to more accurately record early time points. TfR internalization was stopped by adding ice-cold acid wash buffer (0.5 M NaCl/0.2 M acetic acid) to strip residual surface transferrin or anti-CD71 antibody. Cells were then washed twice with cold PBS and analyzed by flow cytometry. The rate of TfR endocytosis was determined from the slope of internalization measured between 3 and 5 minute time points and was normalized to the control vector. The GFP-positive gated cell population was analyzed for CD71 expression or bound transferrin. All experiments contained empty vector controls for comparison.

Western Blots

Western blots were performed according to standard protocols. Proteins were detected by either alkaline phosphatase chemiluminescence methods or by quantitative immunoblot methods using an Odyssey infrared fluorescence imaging system (Li-Cor Biosciences, Lincoln, NB, USA).

Picalmfit1 Mice

ENU saturation mutagenesis studies originally resulted in the identification of five independent fit1 mutations of varying severity [40]. Characterization of hematopoietic and iron deficiency phenotypes has previously been described in mice bearing an allele of intermediate severity, fit14R (formerly fit14226SB) [9][11]. We chose to focus on the most severe allele, fit15R (formerly fit14397SB), which results in a severely truncated and nonfunctional PICALM protein [7], [8]. Picalm+/Picalmfit1–5R (hereafter, fit1+/−) mice were obtained from Oak Ridge National Laboratory (Oak Ridge, TN, USA) and housed under normal conditions in accordance with the Duke University Institutional Animal Care and Use Committee.

Mouse Embryonic Fibroblast Preparation

Heterozygous Picalm+/Picalmfit1–5R mice were bred to generate homozygous mutant (Picalmfit1–5R/Picalmfit1–5R; herafter referred to as PicalmNULL or Null) and WT (Picalm+/Picalm+) embryos, which were harvested at 14.5 dpc. After removal of cranial (used for genotype screening) and liver tissue, the embryo was minced using a sterile razor blade. Following trypsinization, cells were serially replated several times. Within a week following extraction, primary MEFs were either analyzed or immortalized using SV40 T/t antigen. In all experiments, at least one PicalmNULL (Null) and one WT cell line was generated from the same pregnancy, and kept under identical conditions. The following immortalized MEFs were established from littermates from three separate pregnancies: pregnancy #1–3T (Null) and 7T (WT); pregnancy #2–20T (WT), 24T (Null), and 29T (Null); and pregnancy #3–31T (Null) and 33T (WT).

Co-immunoprecipitation Assay

HEK293T cells were harvested 48 hours after transfection, and protein concentration was quantified by Bradford Assay (Thermo). One µL of anti-GFP antibody was added to 500 ng of total protein and incubated overnight at 4°C. Protein-G-Sepharose (GE Life Sciences) was added to the immunoprecipitates for 2 hr at 4°C. After three washes with lysis buffer, immunoprecipitates were analyzed by western blotting.

Quantitative RT-PCR Analysis

Total RNA was isolated from MEFs using RNeasy Mini Kit (Qiagen, Valencia, CA, USA). Up to 1 µg of total RNA was treated with RNase-free DNase I and reverse transcribed using SuperScript II (Invitrogen) according to the manufacturer’s protocol. The 20 µL RT reaction was then diluted with 100 µL RNase-free H2O. One µL of diluted cDNA was used as template for quantitative PCR amplification using iQ Multiplex Powermix (Bio-Rad, Hercules, CA, USA) for 40 cycles according to the manufacturer’s protocol. Amplification data was collected using the iQ5 Optical System (Bio-Rad). The expression level of transferrin receptor transcript was quantified relative to the expression levels of the endogenous housekeeping genes, GAPDH (Sense: 5′-CCTGGAGAAACCTGCCAAGTATG-3′; Antisense: 5′-AGAGTGGGAGTTGCTGTTGAAGTC-3′) and β2M (Sense: 5′-ACCGGCCTGTATGCTATCCAGAAA-3; Antisense: 5′-GGTGAATTCAGTGTGAGCCAGGAT-3′) by the comparative threshold cycle (CT) method [61]. For transferrin receptor, the sense primer was 5′-TCATGAGGGAAATCAATGATCGTA-3′, and the antisense primer was 5′-GCCCCAGAAGATATGTCGGAA-3′.

Intracellular Iron Measurement

The relative amount of intracellular iron was measured in MEFs using two separate approaches. First, total cellular iron content was determined by plating 10 million cells in 5 × 150 cm2 flasks the day prior to harvesting. On the following day, cells were trypsinized, washed, counted, and pelleted. Cell pellets were treated with nitric acid and analyzed by mass spectroscopy at the Soil Lab at North Carolina State University (Raleigh, NC). Iron concentration was normalized to represent the value per cell. Second, the labile iron pool was measured indirectly using the fluorophor Phen Green SK (PGSK – Invitrogen). All MEFs were plated at the same density the day before analysis. A total of 100,000 trypsinized cells were aliquoted in duplicates or triplicates, and 10 µM PGSK was added to each sample in the same final volume. Cells were incubated at 37°C for 30 minutes, pelleted, washed three times with HBSS, and analyzed by flow cytometry. Samples were run without PGSK and background fluorescence was subtracted from the PGSK fluorescence.

Iron Supplementation and Chelation

For iron supplementation studies, non-immortalized MEFs were plated at a concentration of 50,000 cells/well in a 12 well plate with or without 50 µM ferric ammonium citrate (FAC) added both initially and on day 2 after plating. Cell proliferation was measured by counting viable cells following trypsinization at days two and five by flow cytometry.

For iron chelation experiments, immortalized MEFs were plated at a concentration of 25,000 cells/well in a 12 well plate with or without 2.5 µM deferoxamine (DFO) added on day 0 and day 2 after plating. Viable cells were counted at days two and four.

Statistical Analysis and Preparation of Figures

We used the Wilcoxon rank-sum (Mann-Whitney) test to compare observations among groups, unless otherwise indicated. P values <0.05 were considered to be statistically significant. Data analysis and presentation were performed using Microsoft Excel and Stata 12 (College Station, TX). Figures were assembled in Adobe Photoshop.

Supporting Information

Figure S1.

PICALM carboxy-terminal residues are required for inhibition of Tf endocytosis and clathrin binding. (A) TfR internalization in HEK293 cells transiently transfected with PICALM deletion constructs is shown relative to empty vector control (GFP; upper panel). Nexp  = 4. *p<0.002 compared with GFP vector. Lower panel shows corresponding Western blot of proteins co-immunoprecipitated using an anti-GFP antibody followed by immunoblotting with anti-CHC antibody. (B) TfR internalization in HEK293 cells transiently transfected with PICALM NAAIRS mutagenesis constructs is shown relative to empty vector control (GFP; upper panel). Point mutants are designated by the amino acids targeted by NAAIRS mutagenesis (e.g. aa 584–589 PTTAWN were mutated to NAAIRS). Nexp  = 4. *p<0.002 compared with GFP vector. Lower panel shows corresponding Western blot of proteins co-immunoprecipitated using an anti-GFP antibody followed by immunoblotting with anti-CHC antibody. PICALM band (lane 1) was originally at the far right end of the gel and was moved to the left for clarity. (C) Schematic diagram showing location of NAAIRS mutants in PICALM C-terminus. Lower panel illustrates amino acid sequence of C-terminal PICALM, with numbers above sequence corresponding to PICALM amino acids 583–652. Streteches of six consecutive amino acids that were mutated to NAAIRS (asparagiNe-Alanine-Alanine-Isoleucine-aRginine-Serine) are indicated by square brackets above or below the sequence; these residues correspond to those shown in Figure S2B.


Figure S2.

Characterization of PICALM-deficient cell lines. (A) Picalm PCR (upper) and immunoblot with anti-PICALM antibody (lower) confirm the absence of Picalm or PICALM expression in 4 PicalmNULL MEF lines (3T, 24T, 29T, 31T) compared with MEF lines derived from wildtype littermates (7T, 20T, 33T). Native PICALM protein typically appears as a doublet on immunoblots, with variable intensity of upper and lower bands. (B) Immunoblot of PICALM and β-actin protein in WT MEFs infected with shRNA vectors to knock down Picalm expression (shRNA4, shRNA5), control shRNA, or uninfected WT MEFs. (C) Immunoblot of native PICALM and β-actin in HEK293 cells stably transfected with shRNA4 or shRNA Control constructs, demonstrating knockdown of PICALM in comparison with β-actin. (D) Surface TfR expression in shRNA transduced HEK293 cells. Nexp  = 7. *p<0.001 compared with shRNA Control. (E) TfR internalization in HEK293 cells that express shRNA4 or shRNA Control retroviruses. Nexp  =  5. *p  = 0.001 at 3 min, p = 0.006 at 6 min compared with shRNA Control.


Figure S3.

Retroviral expression of PICALM in four independently derived PicalmNULL MEF cell lines rescues TfR surface expression and endocytosis. (A) Kinetics of TfR internalization in independently derived immortalized PicalmNULL lines (3T, 24T, 29T, 31T). Percentage of surface TfR internalized is shown for untransfected (Null), empty vector transfected (Control) and PICALM-transfected MEFs. (B) Relative surface TfR expression in four immortalized MEF PICALM-deficient MEF lines (3T, 24T, 29T, and 31T) derived from three different pregnancies. Surface TfR levels of empty vector transfected (Control) and PICALM-transfected MEFs are shown relative to untransfected (None) MEFs.



We appreciate the thoughtful comments and suggestions of Oren Becher, Lisa Crose, Corinne Linardic, and Rob Wechsler-Reya and the statistical expertise of Christoph Hornik.

Author Contributions

Conceived and designed the experiments: PBS JLH AEC NBP JAW CPL DSW. Performed the experiments: PBS JLH AEC NBP JAW CPL. Analyzed the data: PBS JLH AEC NBP MBA JAW CPL DSW. Contributed reagents/materials/analysis tools: MLK. Wrote the paper: PBS MBA MLK CPL DSW.


  1. 1. Dreyling MH, Martinez-Climent JA, Zheng M, Mao J, Rowley JD, et al. (1996) The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family. Proc Natl Acad Sci USA 93: 4804–4809.
  2. 2. Tebar F, Bohlander SK, Sorkin A (1999) Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic. Mol Biol Cell 10: 2687–2702.
  3. 3. Caudell D, Aplan PD (2008) The role of CALM-AF10 gene fusion in acute leukemia. Leukemia 22: 678–685.
  4. 4. Meyerholz A, Hinrichsen L, Groos S, Esk P-C, Brandes G, et al. (2005) Effect of clathrin assembly lymphoid myeloid leukemia protein depletion on clathrin coat formation. Traffic 6: 1225–1234.
  5. 5. Mettlen M, Stoeber M, Loerke D, Antonescu CN, Danuser G, et al. (2009) Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits. Mol Biol Cell 20: 3251–3260.
  6. 6. Huang F, Khvorova A, Marshall W, Sorkin A (2004) Analysis of clathrin-mediated endocytosis of epidermal growth factor receptor by RNA interference. J Biol Chem 279: 16657–16661.
  7. 7. Klebig ML, Wall MD, Potter MD, Rowe EL, Carpenter DA, et al. (2003) Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice. Proc Natl Acad Sci USA 100: 8360–8365.
  8. 8. Potter MD, Shinpock SG, Popp RA, Godfrey V, Carpenter DA, et al. (1997) Mutations in the murine fitness 1 gene result in defective hematopoiesis. Blood 90: 1850–1857.
  9. 9. Schultze AE, Schaeffer DO, Potter MD, Johnson DK (1997) Alterations in Growth, Hematopoiesis and Serum Chemistry Profiles in fitness 14226SB Mutant Mice. Comp Hematol Int 7: 143–151.
  10. 10. Schultze AE, Poppenga RH, Johnson DK (1998) Alterations in Serum and Tissue Iron Profiles Associated with Mutations in the fitness 14226SB Locus of Mice. Comp Hematol Int 8: 72–76.
  11. 11. Schultze AE, McEntee MF, Daniel GB, Johnson DK (1999) Radiographic, histologic, and cytologic lesions associated with mutations in the fitness1(4226SB) locus of mice. Lab Animal Sci 49: 260–268.
  12. 12. Vecchi M, Polo S, Poupon V, van de Loo JW, Benmerah A, et al. (2001) Nucleocytoplasmic shuttling of endocytic proteins. J Cell Biol 153: 1511–1517.
  13. 13. Archangelo LF, Glasner J, Krause A, Bohlander SK (2006) The novel CALM interactor CATS influences the subcellular localization of the leukemogenic fusion protein CALM/AF10. Oncogene 25: 4099–4109.
  14. 14. Bushlin I, Petralia RS, Wu F, Harel A, Mughal MR, et al. (2008) Clathrin assembly protein AP180 and CALM differentially control axogenesis and dendrite outgrowth in embryonic hippocampal neurons. J Neurosci 28: 10257–10271.
  15. 15. Petralia RS, Yao PJ (2007) AP180 and CALM in the developing hippocampus: expression at the nascent synapse and localization to trafficking organelles. J Compar Neurol 504: 314–327.
  16. 16. Schwartz CM, Cheng A, Mughal MR, Mattson MP, Yao PJ (2010) Clathrin assembly proteins AP180 and CALM in the embryonic rat brain. J Compar Neurol 518: 3803–3818.
  17. 17. Yao PJ, Petralia RS, Bushlin I, Wang Y, Furukawa K (2005) Synaptic distribution of the endocytic accessory proteins AP180 and CALM. J Compar Neurol 481: 58–69.
  18. 18. Morgan JR, Zhao X, Womack M, Prasad K, Augustine GJ, et al. (1999) A role for the clathrin assembly domain of AP180 in synaptic vesicle endocytosis. J Neurosci 19: 10201–10212.
  19. 19. Kalthoff C, Alves J, Urbanke C, Knorr R, Ungewickell EJ (2002) Unusual structural organization of the endocytic proteins AP180 and epsin 1. J Biol Chem 277: 8209–8216.
  20. 20. Ford MG, Pearse BM, Higgins MK, Vallis Y, Owen DJ, et al. (2001) Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. Science 291: 1051–1055.
  21. 21. Kim HL, Kim JA (2000) Purification of clathrin assembly protein from rat liver. Exp Molec Medicine 32: 222–226.
  22. 22. Morgan JR, Prasad K, Hao W, Augustine GJ, Lafer EM (2000) A conserved clathrin assembly motif essential for synaptic vesicle endocytosis. J Neurosci 20: 8667–8676.
  23. 23. Huh JY, Chung S, Oh D, Kang MS, Eom HS, et al. (2010) Clathrin assembly lymphoid myeloid leukemia-AF10-positive acute leukemias: a report of 2 cases with a review of the literature. Korean J Lab Medicine 30: 117–121.
  24. 24. Wechsler DS, Engstrom LD, Alexander BM, Motto DG, Roulston D (2003) A novel chromosomal inversion at 11q23 in infant acute myeloid leukemia fuses MLL to CALM, a gene that encodes a clathrin assembly protein. Genes Chromosom Cancer 36: 26–36.
  25. 25. Kumon K, Kobayashi H, Maseki N, Sakashita A, Sakurai M, et al. (1999) Mixed-lineage leukemia with t(10;11)(p13;q21): an analysis of AF10-CALM and CALM-AF10 fusion mRNAs and clinical features. Genes Chromosom Cancer 25: 33–39.
  26. 26. Asnafi V, Radford-Weiss I, Dastugue N, Bayle C, Leboeuf D, et al. (2003) CALM-AF10 is a common fusion transcript in T-ALL and is specific to the TCRgammadelta lineage. Blood 102: 1000–1006.
  27. 27. Savage NM, Kota V, Manaloor EJ, Kulharya AS, Pierini V, et al. (2010) Acute leukemia with PICALM-MLLT10 fusion gene: diagnostic and treatment struggle. Cancer Genet Cytogenet 202: 129–132.
  28. 28. Deshpande AJ, Cusan M, Rawat VP, Reuter H, Krause A, et al. (2006) Acute myeloid leukemia is propagated by a leukemic stem cell with lymphoid characteristics in a mouse model of CALM/AF10-positive leukemia. Cancer Cell 10: 363–374.
  29. 29. Harold D, Abraham R, Hollingworth P, Sims R, Gerrish A, et al. (2009) Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer's disease. Nature Genet 41: 1088–1093.
  30. 30. Nordstedt C, Caporaso GL, Thyberg J, Gandy SE, Greengard P (1993) Identification of the Alzheimer beta/A4 amyloid precursor protein in clathrin-coated vesicles purified from PC12 cells. J Biol Chem 268: 608–612.
  31. 31. Jones L, Harold D, Williams J (2010) Genetic evidence for the involvement of lipid metabolism in Alzheimer's disease. Biochim Biophys Acta 1801: 754–761.
  32. 32. Seshadri S, Fitzpatrick AL, Ikram MA, DeStefano AL, Gudnason V, et al. (2010) Genome-wide analysis of genetic loci associated with Alzheimer disease. JAMA 303: 1832–1840.
  33. 33. Jun G, Naj AC, Beecham GW, Wang L-S, Buros J, et al. (2010) Meta-analysis confirms CR1, CLU, and PICALM as alzheimer disease risk loci and reveals interactions with APOE genotypes. Arch Neurol 67: 1473–1484.
  34. 34. Wilson IA, Haft DH, Getzoff ED, Tainer JA, Lerner RA, et al. (1985) Identical short peptide sequences in unrelated proteins can have different conformations: a testing ground for theories of immune recognition. Proc Natl Acad Sci USA 82: 5255–5259.
  35. 35. Esposito BP, Epsztejn S, Breuer W, Cabantchik ZI (2002) A review of fluorescence methods for assessing labile iron in cells and biological fluids. Anal Biochem 304: 1–18.
  36. 36. Kaplan J, Jordan I, Sturrock A (1991) Regulation of the transferrin-independent iron transport system in cultured cells. The J Biol Chem 266: 2997–3004.
  37. 37. Hao W, Luo Z, Zheng L, Prasad K, Lafer EM (1999) AP180 and AP-2 interact directly in a complex that cooperatively assembles clathrin. J Biol Chem 274: 22785–22794.
  38. 38. Harel A, Wu F, Mattson MP, Morris CM, Yao PJ (2008) Evidence for CALM in directing VAMP2 trafficking. Traffic 9: 417–429.
  39. 39. Miller SE, Sahlender DA, Graham SC, Honing S, Robinson MS, et al. (2011) The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM. Cell 147: 1118–1131.
  40. 40. Potter MD, Klebig ML, Carpenter DA, Rinchik EM (1995) Genetic and physical mapping of the fitness 1 (fit1) locus within the Fes-Hbb region of mouse chromosome 7. Mamm Genome 6: 70–75.
  41. 41. Suzuki M, Tanaka H, Tanimura A, Tanabe K, Oe N, et al. (2012) The Clathrin Assembly Protein PICALM Is Required for Erythroid Maturation and Transferrin Internalization in Mice. PloS one 7: e31854.
  42. 42. Casey JL, Koeller DM, Ramin VC, Klausner RD, Harford JB (1989) Iron regulation of transferrin receptor mRNA levels requires iron-responsive elements and a rapid turnover determinant in the 3′ untranslated region of the mRNA. EMBO J 8: 3693–3699.
  43. 43. Silliman CC, McGavran L, Wei Q, Miller LA, Li S, et al. (1998) Alternative splicing in wild-type AF10 and CALM cDNAs and in AF10-CALM and CALM-AF10 fusion cDNAs produced by the t(10;11)(p13–14;q14-q21) suggests a potential role for truncated AF10 polypeptides. Leukemia 12: 1404–1410.
  44. 44. Di Fiore PP, Gill GN (1999) Endocytosis and mitogenic signaling. Curr Opin Cell Biol 11: 483–488.
  45. 45. Di Fiore PP, De Camilli P (2001) Endocytosis and signaling. an inseparable partnership. Cell 106: 1–4.
  46. 46. Di Fiore PP (2009) Endocytosis, signaling and cancer, much more than meets the eye. Mol Oncol 3: 273–279.
  47. 47. Hupalowska A, Miaczynska M (2012) The new faces of endocytosis in signaling. Traffic 13: 9–18.
  48. 48. Richardson DR, Kalinowski DS, Lau S, Jansson PJ, Lovejoy DB (2009) Cancer cell iron metabolism and the development of potent iron chelators as anti-tumour agents. Biochim Biophys Acta 1790: 702–717.
  49. 49. Connor JR, Snyder BS, Beard JL, Fine RE, Mufson EJ (1992) Regional distribution of iron and iron-regulatory proteins in the brain in aging and Alzheimer's disease. J Neurosci Res 31: 327–335.
  50. 50. Zecca L, Youdim MB, Riederer P, Connor JR, Crichton RR (2004) Iron, brain ageing and neurodegenerative disorders. Nature Rev Neurosci 5: 863–873.
  51. 51. Smith MA, Zhu X, Tabaton M, Liu G, McKeel DW Jr, et al. (2010) Increased iron and free radical generation in preclinical Alzheimer disease and mild cognitive impairment. J Alzheimer's Dis 19: 363–372.
  52. 52. Oshiro S, Morioka MS, Kikuchi M (2011) Dysregulation of iron metabolism in Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Adv Pharmacol Sci 2011: 378278.
  53. 53. Carrasquillo MM, Belbin O, Hunter TA, Ma L, Bisceglio GD, et al. (2010) Replication of CLU, CR1, and PICALM associations with alzheimer disease. Arch Neurol 67: 961–964.
  54. 54. Corneveaux JJ, Myers AJ, Allen AN, Pruzin JJ, Ramirez M, et al. (2010) Association of CR1, CLU and PICALM with Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals. Hum Mol Genet 19: 3295–3301.
  55. 55. Treusch S, Hamamichi S, Goodman JL, Matlack KE, Chung CY, et al. (2011) Functional Links Between Abeta Toxicity, Endocytic Trafficking, and Alzheimer's Disease Risk Factors in Yeast. Science 334(6060): 1241–1245.
  56. 56. Morita S, Kojima T, Kitamura T (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7: 1063–1066.
  57. 57. Kingston RE, Chen CA, Okayama H (2001) Calcium phosphate transfection. Cur Protocols Immunol Chapter 10: Unit 10.13.
  58. 58. Du C, Redner RL, Cooke MP, Lavau C (1999) Overexpression of wild-type retinoic acid receptor alpha (RARalpha) recapitulates retinoic acid-sensitive transformation of primary myeloid progenitors by acute promyelocytic leukemia RARalpha-fusion genes. Blood 94: 793–802.
  59. 59. Armbruster BN, Banik SS, Guo C, Smith AC, Counter CM (2001) N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo. Molecular and Cell Biol 21: 7775–7786.
  60. 60. Ngo VN, Davis RE, Lamy L, Yu X, Zhao H, et al. (2006) A loss-of-function RNA interference screen for molecular targets in cancer. Nature 441: 106–110.
  61. 61. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nature Protocols 3: 1101–1108.