Opiates, like other addictive drugs, elevate forebrain dopamine levels and are thought to do so mainly by inhibiting GABA neurons near the ventral tegmental area (VTA), in turn leading to a disinhibition of dopamine neurons. However, cholinergic inputs from the laterodorsal (LDT) and pedunculopontine (PPT) tegmental nucleus to the VTA and substantia nigra (SN) importantly contribute, as either LDT or PPT lesions strongly attenuate morphine-induced forebrain dopamine elevations. Pharmacological blockade of muscarinic acetylcholine receptors in the VTA or SN has similar effects. M5 muscarinic receptors are the only muscarinic receptor subtype associated with VTA and SN dopamine neurons. Here we tested the contribution of M5 muscarinic receptors to morphine-induced dopamine elevations by measuring nucleus accumbens dopamine efflux in response to intra-VTA morphine infusion using in vivo chronoamperometry. Intra-VTA morphine increased nucleus accumbens dopamine efflux in urethane-anesthetized wildtype mice starting at 10 min after infusion. These increases were absent in M5 knockout mice and were similarly blocked by pre-treatment with VTA scopolamine in wildtype mice. Furthermore, in wildtype mice electrical stimulation of the PPT evoked an initial, short-lasting increase in striatal dopamine efflux, followed 5 min later by a second prolonged increase in dopamine efflux. In M5 knockout mice, or following systemic pre-treatment with scopolamine in wildtype mice, the prolonged increase in striatal dopamine efflux was absent. The time course of increased accumbal dopamine efflux in wildtype mice following VTA morphine was consistent with both the prolonged M5-mediated excitation of striatal dopamine efflux following PPT electrical stimulation and accumbal dopamine efflux following LDT electrical stimulation. Therefore, M5 receptors appear critical for prolonged PPT excitation of dopamine efflux and for dopamine efflux induced by intra-VTA morphine.
Citation: Steidl S, Miller AD, Blaha CD, Yeomans JS (2011) M5 Muscarinic Receptors Mediate Striatal Dopamine Activation by Ventral Tegmental Morphine and Pedunculopontine Stimulation in Mice. PLoS ONE 6(11): e27538. https://doi.org/10.1371/journal.pone.0027538
Editor: Antonio Verdejo García, University of Granada, Spain
Received: September 2, 2011; Accepted: October 19, 2011; Published: November 15, 2011
Copyright: © 2011 Steidl et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a Canadian Institutes of Health Research (CIHR) grant to JSY and a Natural Sciences and Engineering Research Council of Canada Graduate Scholar doctoral scholarship (NSERC CGS-D) to SS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Dopamine neurotransmission in the forebrain plays an important role in the reinforcing effects of drugs of abuse . Opiate drugs like heroin and morphine are thought to affect dopaminergic neurotransmission through their actions near the ventral tegmental area (VTA), where opiates inhibit local gamma-aminobutyric acid (GABA) neurons by acting on mu opioid receptors , leading to increased firing of dopamine neurons via disinhibition –, and increased accumbal dopamine –.
Opiate-induced dopamine elevations in the forebrain also depend, however, on cholinergic inputs from the pedunculopontine (PPT) and laterodorsal (LDT) tegmental nuclei that excite midbrain dopamine neurons –. In rats, PPT or LDT lesions reduce most of the striatal or accumbal dopamine increases following systemic morphine –. Additionally, VTA or SN infusions of the non-selective muscarinic receptor antagonist scopolamine similarly reduce most striatal or accumbal dopamine increases following systemic morphine . PPT lesions also block morphine place preference  and heroin self-administration  in drug-naïve rats. The pathways through which the PPT mediates the acute rewarding effects of opiates and dopamine activation (i.e., via descending or ascending projections) are not clear, but cholinergic mechanisms are important. For example, intra-VTA infusion of the muscarinic antagonist atropine reduces morphine place preference in rats .
The M5 muscarinic acetylcholine receptor subtype, associated with dopamine neurons, is most important for cholinergic excitation of VTA and SN dopamine neurons –. In mice, for example, accumbal dopamine efflux following LDT electrical stimulation occurs in three phases. The strongest dopamine efflux in the long third phase is absent in M5 knockout mice .
M5 muscarinic excitation is also critical for opiate functions. M5 knockout mice show reduced morphine-induced accumbal dopamine efflux as measured by microdialysis, and reduced morphine conditioned place preference . Also, morphine-induced locomotion is reduced by ∼40–50% in M5 knockout mice . M5 muscarinic receptors in the VTA, therefore, contribute strongly to both the stimulant and rewarding effects of morphine.
The goal of the current studies was to better understand the role of the PPT and M5 receptors in opiate-induced dopamine activation. Increases in accumbal dopamine efflux following VTA infusions of morphine were absent in M5 knockout mice. Prolonged PPT-evoked striatal dopamine efflux, which has not been previously characterized in mice, was also reduced in M5 knockout mice. In both cases, M5-mediated increases in forebrain dopamine efflux followed a similar time course. Our results suggest that VTA opiates may indirectly activate dopamine neurons through a mechanism involving a PPT/LDT cholinergic relay.
Experiment 1: Intra-VTA morphine-induced accumbal dopamine efflux in wildtype and M5 knockout mice
Figure 1A shows VTA injection sites identified from brains of individual wildtype and M5 knockout mice in Experiment 1. Injection sites in the various treatment conditions were confined within the boundaries of the VTA and were spread along its rostro-caudal extent. Recording sites in the nucleus accumbens (Fig. 1B) were confined to the core region between 0.98 and 1.54 mm anterior to bregma. Only mice with both VTA injection and accumbens recording sites were used for statistical analysis.
In both (A) and (B), open circles show placements from M5 knockout mice (n = 4) treated with 50 ng intra-VTA morphine, closed circles show placements from M5 knockout mice treated with 0.3 µl intra-VTA saline (n = 4), open triangles show placements from wildtype mice (n = 4) treated with 50 ng intra-VTA morphine, closed triangles show placements from wildtype mice (n = 4) treated with 0.3 µl intra-VTA saline, stars show placements from wildtype mice (n = 6) pre-treated with 50 µg intra-VTA scopolamine followed by 50 ng intra-VTA morphine, and open squares show placements from wildtype mice (n = 4) pre-treated with 1 mg/kg (i.p.) naltrexone followed by 50 ng intra-VTA morphine. For clarity infusion sites are shown on both left and right side of the brain, but VTA infusions were always made on the left side of the brain. Numbers to the left of or above individual sections show distance in mm from bregma. Sections were adapted from the atlas of Paxinos and Franklin .
VTA morphine increases accumbal dopamine efflux in wildtype, but not in M5 knockout, mice.
Compared to saline, intra-VTA infusions of morphine (50 ng) in wildtype mice induced a delayed increase in accumbal dopamine efflux that began 16.5±7.9 min after infusion and steadily increased over the course of the following 2 hrs. Subsequently, dopamine efflux leveled off over a 50-min period, but never decreased back to baseline levels (Fig. 2A). By contrast, in M5 knockout mice the same dose of intra-VTA morphine induced a slight decrease in accumbal dopamine efflux relative to saline, starting at 5.9±2.0 min, that returned to pre-injection baseline levels by 92.0±9.8 min and subsequently showed a slight increase to above baseline over the final 30 min (Fig. 2B).
In all cases, thick lines represent mean dopamine oxidation current across mice, and thin lines represent ±SEM. (A) Mean change in accumbal dopamine efflux in wildtype mice following 50 ng intra-VTA morphine (black line, n = 4) or 0.3 µl intra-VTA saline (gray line, n = 4). (B) Mean change in accumbal dopamine efflux in M5 knockout mice following 50 ng intra-VTA morphine (black line, n = 4) or 0.3 µl intra-VTA saline (gray line, n = 4). (C) Effects of pre-treatment with 50 µg intra-VTA scopolamine on accumbal dopamine efflux induced by 50 ng intra-VTA morphine in wildtype mice (black line, n = 6). For comparison, data from (A) showing mean changes in accumbal dopamine efflux induced by 50 ng intra-VTA morphine without any pre-treatment in wildtype mice are reproduced (gray line, n = 4). (D) Effects of pre-treatment with naltrexone (1 mg/kg, i.p.) 5 min prior to 50 ng intra-VTA morphine in wildtype mice (black line, n = 4). For comparison, data from (A) showing mean changes in accumbal dopamine efflux induced by 50 ng intra-VTA morphine without any pre-treatment in wildtype mice are reproduced (gray line, n = 4).
A between-within repeated-measures ANOVA with genotype as the between-subjects factor and time as the within-subjects factor revealed a significant interaction between genotype and time (F (344, 1376) = 11.59, p<0.0001), confirming that the temporal profile of accumbal dopamine efflux induced by intra-VTA morphine was different between wildtype and M5 knockout mice. Post-hoc analysis using Fischer's LSD test showed significantly lower accumbal dopamine efflux in M5 knockout mice at every 30-sec time point between 32 and 165 min (p's<0.05 to p's<0.001).
VTA scopolamine prevents increases in accumbal dopamine efflux induced by intra-VTA morphine in wildtype mice.
In wildtype mice, intra-VTA pre-treatment with scopolamine (50 µg) prevented the increase in accumbal dopamine efflux typically observed following intra-VTA morphine (50 ng) (Fig. 2C). Most interestingly, the intra-VTA combination of scopolamine and morphine in wildtype mice resulted in a decrease in accumbal dopamine efflux, comparable to that observed following intra-VTA morphine alone in M5 knockout mice (Fig. 2B vs. 2C). A two-way between-within repeated-measures ANOVA with drug pre-treatment in wildtype mice (VTA scopolamine vs. no pre-treatment) as the between-subjects factor and time as the within-subjects factor revealed a significant interaction between pre-treatment and time, F (306, 2448) = 11.22, p<0.000001, indicating that the temporal profile of dopamine efflux varied as a function of VTA pre-treatment. Post-hoc analysis using Fischer's LSD test showed that scopolamine pre-treatment significantly reduced accumbal dopamine efflux at every 30-sec time point between 40 and 165 min (all p's<0.05).
Increased accumbal dopamine efflux induced by intra-VTA morphine is prevented by systemic naltrexone in wildtype mice.
Figure 2D shows accumbal dopamine efflux induced by intra-VTA morphine (50 ng) in wildtype mice that were pre-treated with systemic naltrexone (1 mg/kg i.p.). VTA morphine infusion delivered 5 min after systemic naltrexone resulted in a decrease in accumbal dopamine efflux to below baseline levels that peaked at 33.7±4.7 min and returned to pre-injection baseline levels by 57.8±9.3 min. Over the course of the next 1.5 hrs, accumbal dopamine efflux gradually increased but always remained below levels observed in VTA morphine-infused wildtype mice that were not pre-treated with naltrexone. Thus, systemic naltrexone pre-treatment effectively blocked the steady morphine-induced increase observed in wildtype mice starting between 10–20 min, but was unable to block a small increase in dopamine efflux starting at approximately 1 hr. A two-way between-within repeated measures ANOVA with group (naltrexone pre-treatment vs. no pre-treatment) as the between-subjects factor and time as the within-subjects factor revealed a significant interaction between group and time (F (305, 1830) = 3.01, p<0.00001). This indicates that the temporal profile of accumbal dopamine efflux across the 2.5-hr recording period was different between mice pre-treated with naltrexone and mice that received no pre-treatment. Post-hoc analysis using Fischer's LSD test showed significantly reduced accumbal dopamine efflux in mice pre-treated with naltrexone at every 30-sec time point between 54 and 148 min (all p's<0.05).
Experiment 2: PPT-evoked striatal dopamine efflux in wildtype and M5 knockout mice
PPT stimulating electrodes in both wildtype and M5 knockout mice were distributed along the rostro-caudal extent of the PPT as shown on sagittal sections (Fig. 3A). Recording electrodes were confined within the boundaries of the striatum, between 0.98–1.70 mm anterior to bregma in both wildtype and M5 knockout mice (Fig. 3B). At the current used in these experiments, the stimulation was estimated to activate unmyelinated PPT cholinergic axons (conduction velocities of 0.4–1.2 m/s) within a distance of 0.75 mm of the electrode tip –.
(A) Sagittal sections of the mouse brain showing tip placements of PPT stimulating electrodes in wildtype (open triangles, n = 7) and M5 knockout (open circles, n = 7) mice. Numbers above individual sections indicate lateral distance in mm from the midline. (B) Coronal sections of the mouse brain showing tip placements of recording electrodes in wildtype (open triangles, n = 7) and M5 knockout (open circles, n = 7) mice. Numbers above individual sections show distance in mm from bregma. Sections are adapted from the atlas of Paxinos and Franklin .
M5 knockout mice show reduced PPT-evoked striatal dopamine efflux.
Electrical stimulation of the PPT in wildtype mice induced two increases in striatal dopamine efflux separated by a trough near 5 min post-stimulation. The first increase began with the first measurement at 30 sec after stimulation and peaked at a mean of 1.25 min (Fig. 4A, black line and Table 1). This was followed by a more gradual decline in dopamine efflux (trough) that reached a minimum at 4.25 min. At ∼5–6 min after PPT stimulation, dopamine efflux began to increase again (second increase). This second increase peaked at 33 min and had a mean duration of 68.1±3.7 min, as measured by the time from the trough minimum to the time the signal returned to pre-stimulation baseline levels. This second increase was ∼3.4 times larger than the first increase in dopamine efflux and over 30 times longer in duration.
In all cases thick lines represent mean oxidation current corresponding to striatal dopamine efflux across mice, and thin lines represent ± SEM. (A) PPT-evoked striatal dopamine efflux in wildtype (black trace, n = 7) and M5 knockout (gray trace, n = 7) mice. (B) M5 receptor contribution to PPT-evoked striatal dopamine efflux in mice. The graph shows the difference in dopamine efflux between wildtype (n = 7) and M5 knockout (n = 7) mice. (C) Effects of scopolamine (5 mg/kg, i.p.) pre-treatment on PPT-evoked striatal dopamine efflux in 4 of the wildtype mice shown in (A). The black trace shows PPT-evoked dopamine efflux, and the gray trace shows PPT-evoked dopamine efflux following systemic scopolamine. (D) Effects of scopolamine (5 mg/kg, i.p.) pre-treatment on PPT-evoked striatal dopamine efflux in 4 of the M5 knockout mice shown in (A). The black trace shows PPT-evoked dopamine efflux and the gray trace shows PPT-evoked dopamine efflux following systemic scopolamine.
PPT stimulation in M5 knockout mice also induced two increases in striatal dopamine efflux separated by a trough (Fig. 4A, gray line and Table 1). Neither the magnitude nor the time of the first increase or the subsequent trough in striatal dopamine efflux differed significantly between M5 knockout and wildtype mice (all p's>0.1). By comparison however, the second increase was markedly attenuated in M5 knockout mice, with peak dopamine efflux significantly lower compared to wildtype mice (t(14) = 3.38, p<0.01). The minimum of the trough was always above baseline in both wildtype and M5 knockout mice.
To calculate the overall M5 contribution to PPT-evoked striatal dopamine efflux, the average PPT-evoked change in basal dopamine oxidation current in M5 knockout mice was subtracted from the average change in wildtype mice. This shows that following electrical stimulation of the PPT, M5 receptors mediate a delayed increase in striatal dopamine efflux with the greatest rise occurring between 5 and 22 min, and a peak around 30 min (Fig. 4B). This delayed M5 contribution is consistent with previous studies measuring M5 time course in pilocarpine-induced salivation and in N-methyl-scopolamine binding studies in vitro –.
Systemic scopolamine pre-treatment reduces striatal dopamine efflux in wildtype, but not in M5 knockout, mice.
In wildtype mice, systemic administration of scopolamine 30 min prior to PPT stimulation did not alter the magnitude (t(3) = 1.00, p>0.3), or the time (t(3) = 0.39, p>0.7) of the first increase in striatal dopamine efflux. The trough was slightly enhanced, with the minimum now below baseline, resulting in a statistically significant difference relative to the pre-scopolamine baseline (t(3) = 3.94, p<0.05). Pre-treatment with scopolamine almost completely abolished the second increase in striatal dopamine efflux (t(3) = 7.53, p<0.01) in wildtype mice (Fig. 4C).
In M5 knockout mice (Fig. 4D), systemic administration of scopolamine 30 min prior to PPT stimulation did not affect the magnitude (p>0.5) or the time (p>0.5) of the first increase in striatal dopamine efflux. The trough was also not affected in M5 knockout mice, either in terms of its minimum (p>0.4) or the time of the minimum (p>0.2). Scopolamine had no effect on the already significantly reduced second increase in dopamine efflux in M5 knockout mice (p>0.9).
Increases in accumbal dopamine efflux following intra-VTA morphine were absent in M5 knockout mice and blocked by VTA scopolamine pre-treatment in wildtype mice. In a similar manner, the second increase in striatal dopamine efflux following electrical stimulation of the PPT was absent in M5 knockout mice and blocked by systemic scopolamine pre-treatment in wildtype mice. In both cases M5 receptors mediated a delayed onset, prolonged increase in accumbal and striatal dopamine efflux. Together, these data suggest that M5 receptor activation in the VTA/SN is critical for delayed, long-lasting excitation of dopamine neurons by either intra-VTA morphine or PPT electrical stimulation.
In wildtype mice, morphine infusion into the VTA induced a slow and long-lasting (>2 hrs) increase in accumbal dopamine efflux that began 10–20 min after infusion. This effect was entirely absent in M5 knockout mice. By contrast, nicotine or carbachol in rats has been shown to increase basal dopamine efflux in the accumbens within 1 min following VTA infusion , –, indicating a 10–20 min delay in the activation of dopamine neurons by VTA morphine and consequent detectable increases in accumbal dopamine efflux.
Accumbal dopamine efflux rose steadily from 10–150 min following VTA morphine infusion and then leveled off between 2.5 to 3 hrs post-infusion. Previous chronoamperometric measurement of both i.v. morphine-induced accumbal and striatal dopamine efflux in urethane-anesthetized rats using similar recording electrodes showed a shorter-latency increase in dopamine efflux that peaked at ∼40 min and fully returned to pre-injection baseline levels between 2–3 hours –. Similarly, morphine-induced accumbal dopamine efflux measured by microdialysis in mice showed a peak between 60 and 80 min that gradually returned toward baseline between 2.5–3 hrs , .
The increased accumbal dopamine efflux induced by 50 ng intra-VTA morphine observed in urethane-anesthetized mice is thought to have functional relevance, as mice self-administer a 50 ng dose of morphine into the VTA, and this self-administration has been shown to be reduced by pre-treatment with 4 mg/kg (i.p.) of the non-selective opioid receptor antagonist naloxone . In a similar manner, we observed a marked reduction in the increase in accumbal dopamine efflux following pre-treatment with 1 mg/kg (i.p.) naltrexone. As morphine was infused directly into the VTA, it is likely that systemic naltrexone inhibited increases in dopamine efflux via blockade of VTA opioid receptors. Although naltrexone pre-treatment completely blocked morphine-induced accumbal dopamine efflux for the first 60 min, dopamine efflux recovered to above baseline levels over the course of the next 90 min (see Fig. 2D). Interestingly, pre-treatment with 1 mg/kg naltrexone in B6 wildtype mice has been shown to completely block morphine-induced locomotion (30 mg/kg, i.p.) for the first 90 min, but over the final 30 min of testing locomotor levels increase . Thus, opioid receptors are likely responsible, in a similar way, for both the effects of morphine on locomotion in freely-behaving mice and on accumbal dopamine efflux induced by intra-VTA morphine.
Our previous chronoamperometry study showed that electrical stimulation of the LDT in wildtype mice evoked an initial brief increase in accumbal dopamine efflux, followed by a brief decrease to below baseline levels, and thereafter by a prolonged second increase in accumbal dopamine efflux . The present study showed that electrical stimulation of the PPT in the same wildtype mice also evoked two comparable increases in striatal dopamine efflux. The first increase following PPT stimulation was not affected in M5 knockout mice. The second, prolonged increase began 5–6 min following stimulation, and in marked contrast to wildtype mice, the second increase in striatal dopamine efflux was significantly reduced in M5 knockout mice. In addition, scopolamine pre-treatment in wildtype mice prevented the second increase in striatal dopamine efflux. Together, these data suggest that M5 receptors in the SN – play a critical role in mediating sustained excitatory cholinergic input to the nigrostriatal dopamine system.
The trough in striatal dopamine efflux following PPT stimulation was reduced compared to LDT stimulation. Following LDT stimulation in rats, the trough in accumbal dopamine has been shown to be mediated by M2-like autoreceptors at the level of the LDT . The relatively smaller trough observed here in the striatum suggests that these autoreceptors may not play a significant role in regulating PPT cholinergic inputs to the SN under the current conditions. In addition, the second increase in PPT-evoked striatal dopamine efflux was much larger (∼3.4-fold) and faster (∼5 min) relative to the first increase, compared to LDT-evoked accumbal dopamine efflux (∼2-fold and ∼8 min) described previously . This suggests that the trough in PPT-evoked striatal dopamine efflux in 129 wild-type mice is partly masked by a larger M5-mediated second increase in prolonged striatal dopamine efflux. Inspection of Figure 4B supports this as the M5 contribution to increased striatal dopamine efflux following PPT stimulation began during the trough (i.e. between 5 and 22 min), thus likely masking the inhibitory effect of the trough.
The temporal changes in dopamine efflux following PPT or LDT stimulation provide several similarities to the effects of intra-VTA morphine on accumbal dopamine efflux observed in Experiment 1. Electrical stimulation of the PPT (Experiment 2) increased striatal dopamine efflux with a relatively rapid onset (<30 sec) followed by an M5 receptor-dependent increase in dopamine efflux occurring ∼5 min after stimulation (see Fig. 4B). By comparison, intra-VTA morphine also increased accumbal dopamine efflux in a similar delayed manner, and only in wildtype mice (Fig. 2A). If opiates in the VTA were affecting mesolimbic dopamine neurons exclusively through disinhibition of local GABA neurons , then elevations in basal extracellular dopamine concentrations would be expected to occur faster than 10 min. Instead, the present data suggest that the 10-min onset delay was due to opiate-induced disinhibition of PPT and LDT cholinergic neurons, which, in turn, act more slowly on midbrain dopamine neurons via M5 muscarinic receptors (see Fig. 4B). Opiates can inhibit GABA neurons in the VTA  and descending GABAergic projections to the PPT are important for opiate reward in drug-naïve animals . SN pars reticulata GABA neurons have been shown to directly inhibit cholinergic neurons in the pons  and whole-cell patch clamp recordings have shown that cholinergic neurons in the LDT are inhibited by GABA application . Conceivably, inhibition of GABAergic projections to the PPT and LDT by VTA opiates may, in turn, lead to a disinhibition of ascending PPT/LDT cholinergic inputs to the SN/VTA thereby enhancing dopamine neurotransmission via activation of M5 receptors. The indirect activation of midbrain dopamine neurons through a mesopontine cholinergic relay offers an explanation for the delayed onset action of intra-VTA morphine. This mechanism also explains the inability of intra-VTA morphine to enhance basal accumbal dopamine efflux in M5 knockout mice, as well as the ability of intra-VTA scopolamine to effectively block this increase in wildtype mice.
Our morphine infusions sites tended to be in the caudal half of the VTA (Fig. 1A), so it is possible that the 0.3 µl infusions could have spread to affect GABA neurons in the neighboring rostromedial tegmentum (RMTg), where high levels of mu opioid receptors are found . Similar to the VTA, RMTg neurons can be inhibited by morphine , and RMTg neurons project to, among other places, the VTA and SNc, as well as the PPT .
In conclusion, the present data show that prolonged increases in accumbal dopamine efflux induced by intra-VTA morphine, and prolonged increases in striatal dopamine efflux induced by PPT electrical stimulation both critically depend on M5 muscarinic receptors in the midbrain. Consistent with previous studies –, , the present data further emphasize the importance of M5 receptors in modulating cholinergic excitation of both nigrostriatal and mesolimbic dopaminergic transmission. Previous studies have shown that the PPT/LDT – and muscarinic receptors in the SN/VTA  are critical for morphine-induced dopamine activation. Here we show a complete loss of increased dopamine efflux induced by intra-VTA morphine in M5 knockout mice. Taken together, these data suggest an important role for increased cholinergic input to the VTA in mediating dopamine activation induced by morphine and specifically suggest that M5 receptors are a critical target through which opiates in the VTA activate mesencephalic dopaminergic systems.
Materials and Methods
M5 knockout mice were homozygous mutants for the M5 muscarinic acetylcholine receptor gene. Mice were created using recombinant DNA methods on a mixed 129 SvJ×CD1 background as described by Takeuchi et al. . Homozygous and wildtype mice were used to maintain a colony based on homozygous breeding. In all experiments litters of male homozygous M5 knockout and wildtype mice were age-matched (4–8 months at the time of testing). Eighteen male wildtype and 8 male M5 knockout mice were used in Experiment 1. Seven wildtype and 7 M5 knockout mice were used in Experiment 2. These studies were approved by the University of Toronto Animal Care Committee under protocol # 20007696.
Stearate-modified carbon-paste electrodes
Stearate-modified carbon paste electrodes were constructed according to the methods of Blaha and Jung . Briefly, 75 mg of stearate (99.9% purity, Sigma-Aldrich, St. Louis, MO) were dissolved in 1 ml silicone oil (Sigma-Aldrich, St. Louis, MO) heated to 40°C. To this solution 1 g of graphite powder (particle size <20 µm, Sigma, St. Louis, MO) was added and thoroughly mixed until reaching a paste-like consistency. Electrodes were constructed by packing carbon paste into a 0.5–1 mm well, created by extruding the Teflon coating off a 0.008″ o.d. Teflon-coated stainless-steel wire (Medwire, Mount Vernon, NY) beyond the tip of a ∼10 cm length of wire. The carbon paste was packed into the well tightly by dropping the electrode tip on a glass plate several times. The surface of the electrode tip was investigated under a microscope to ensure that there were no cracks or grooves in the surface of the carbon paste.
Experiment 1: Morphine-induced accumbal dopamine efflux in wildtype and M5 knockout mice
Mice were anesthetized with urethane (1.5 g/kg, i.p.). Each mouse was mounted in a stereotaxic frame (David Kopf Instruments, Tujunga, CA or MyNeuroLab, St. Louis, MO) using rat earbars (Stoelting, Wood Dale, IL) and a mouse head-holder (Stoelting, Wood Dale, IL). Temperature was maintained at 37±0.5°C with a temperature-regulated heating pad (TC-1000; CWE Inc., New York, NY). A single 26 gauge guide cannula (Plastics One, Roanoke, VA) was implanted 1 mm dorsal to the left VTA of each mouse (A/P +0.9 mm from lambda, M/L 0.4 mm D/V −4.4 mm from dura). For VTA infusions a 33 gauge cannula was inserted 1 mm past the tip of the guide cannula, and was connected to a Hamilton microsyringe via Tygon tubing. A single stearate-modified carbon paste recording electrode was implanted into the left nucleus accumbens of each mouse (A/P +1.4 mm from bregma, M/L 0.8 mm, D/V −3.6 mm from dura). A combination Ag/AgCl reference and stainless-steel auxiliary electrode was placed into contact with the contralateral parietal cortex.
Electrochemical recordings and VTA microinfusions.
After in vivo implantation of all electrodes and cannulae, the working characteristics of the recording electrode were evaluated by recording several voltammetric sweeps in the striatum (triangular wave potentials applied from −0.15 to 0.45 V vs. Ag/AgCl; ramp rate 10–20 mV/sec) using an electrometer (EChempro; GMA Technologies, Vancouver, Canada). After confirming the presence of a dopamine peak in the voltammogram, repetitive chronoamperometric measurements of oxidation current were made by applying a potential pulse from −0.15 V to +0.25 V to the recording electrode for 1 sec at 30-sec intervals and monitoring the current for the final 50 ms of each 1-sec pulse . Following at least 30 min of baseline recordings, VTA infusions of morphine (50 ng, 0.3 µl volume) were made and changes in accumbal dopamine oxidation current, corresponding to dopamine efflux, were monitored for 2–3 hrs thereafter. Changes in dopamine efflux following 50 ng intra-VTA morphine were tested in 4 wildtype and 4 M5 knockout mice. In an additional 6 wildtype mice, changes in accumbal dopamine efflux in response to 50 ng intra-VTA morphine were measured following VTA pre-treatment with 50 µg scopolamine 10 min prior to morphine. Changes in accumbal dopamine efflux following 50 ng intra-VTA morphine were also measured in an additional 4 wildtype mice following systemic pre-treatment with naltrexone hydrochloride (1 mg/kg, i.p.) 5 min prior to morphine. Finally, changes in accumbal dopamine oxidation current in response to 0.3 µl VTA saline were measured in 4 wildtype and 4 M5 knockout mice.
Pre-stimulation baseline chronoamperometric currents were normalized to zero current values, with induced changes in oxidation current, corresponding to changes in dopamine efflux, presented as absolute changes in current (increases as positive and decreases as negative). Changes in dopamine efflux following intra-VTA morphine in wildtype and M5 knockout mice, or following combinations of intra-VTA morphine and intra-VTA scopolamine or systemic naltrexone in wildtype mice were analyzed using mixed-model ANOVA. Significant interactions were further analyzed using Fisher's LSD test.
Experiment 2: PPT-evoked striatal dopamine efflux in wildtype and M5 knockout mice
Surgical procedures and chronoamperometric recordings followed those described for Experiment A single concentric, bipolar stimulating electrode (SNE-100; Rhodes Medical Co., Woodland Hills, CA) was implanted into the left PPT of each mouse (A/P −0.5 mm from lambda, M/L 1.2 mm, D/V −2.9 mm from dura) according to the atlas of Paxinos and Franklin (2004), and a single stearate-modified carbon paste electrode was implanted into the left striatum (A/P +1.4 mm from bregma, M/L 1.5 mm, D/V −2.3 mm from dura). A combination Ag/AgCl reference and stainless-steel auxiliary electrode was placed into contact with the contralateral parietal cortex.
PPT electrical stimulation.
Following at least 30 min of baseline recordings, a series of cathodal monophasic current pulses (400 µA, 0.5 ms duration) were delivered to the concentric, bipolar stimulating electrode implanted in the PPT via a programmable pulse generator (Master-8; A.M.P.I., Jerusalem, Israel) in combination with a constant current stimulus isolation unit (Iso-Flex; A.M.P.I., Jerusalem, Israel). Each stimulation of the PPT consisted of a 1 sec, 35 Hz train of 400 µA pulses (1 sec inter-train interval) applied over a 60 sec period (1050 pulses in total). These parameters were designed to mimic spontaneous firing patterns of PPT neurons in awake, naturally aroused animals . In urethane-anesthetized rats these stimulation parameters (at 800 µA) have been shown to evoke a tri-phasic pattern of striatal dopamine efflux .
Systemic drug administration.
Subsequent to monitoring the effects of PPT stimulation on striatal dopamine efflux, a sub-set of mice (4 wildtypes and 4 M5 knockouts) was injected with the non-selective muscarinic receptor antagonist scopolamine hydrobromide (5 mg/kg, i.p.). Thirty min later, the PPT was again stimulated and changes in striatal dopamine oxidation currents were monitored for an additional 80 min.
Pre-stimulation baseline chronoamperometric currents were normalized to zero current values, with stimulated change in oxidation current, corresponding to changes in dopamine efflux, presented as absolute changes in current (increases as positive and decreases as negative). The maximal and minimal PPT-evoked changes in dopamine efflux associated with the two increases and the decrease, respectively, were obtained for individual wildtype and M5 knockout mice. In the case of the 8 mice that were pre-treated with scopolamine, maximal and minimal PPT-evoked changes in dopamine efflux after scopolamine were also obtained and compared to pre-scopolamine PPT-evoked values. Mean peak efflux (in nanoamperes, nA) and peak times (in min) were compared between wildtype and M5 knockout mice (two-tailed unpaired t-test) and before and after scopolamine in all mice (two-tailed paired t-test). The peak time of the second increase in dopamine efflux, which was strongly attenuated in M5 knockout mice, was not statistically analyzed.
To calculate the overall M5 contribution to PPT-evoked striatal dopamine efflux, the average PPT-evoked change in basal dopamine oxidation current in M5 knockout mice was subtracted from the average change in wildtype mice at each 30-sec time point. The calculated differences were then plotted as a function of time following PPT stimulation.
On completion of all experiments mice were killed with a 0.25 ml intracardial injection of urethane. Their brains were removed and put into a 10% formalin solution. All brains were then sectioned on a Vibratome, examined under a light microscope, and compared to atlas sections (Paxinos and Franklin, 2004).
On completion Experiment 2, a PPT lesion was made by passing direct current (100 µA for 5 sec) through the concentric bipolar stimulating electrode. For these brains 0.1% potassium ferricyanide was included in the formalin solution. The iron deposited at the stimulating electrode tip by the electrolytic lesion reacted with the potassium ferricyanide to form a Prussian blue spot, clearly marking the PPT stimulation site.
Morphine sulfate pentahydrate, scopolamine hydrobromide, naltrexone hydrochloride, and urethane were purchased from Sigma Aldrich (St. Louis, MO) and dissolved in sterile 0.9% saline. Drugs were infused into the VTA or systemically injected as their salt weight. Intra-VTA infusions were made at a volume of 0.3 µl, and systemic naltrexone and scopolamine injections at a volume of 10 ml/kg.
We thank Esther Lee, Kasper Podgorski and Silvia Isabella for help with histological analyses. We also thank Drs. Haoran Wang, Derek van der Kooy, and Suzanne Erb for helpful discussion.
Conceived and designed the experiments: SS ADM CDB JSY. Performed the experiments: SS ADM. Analyzed the data: SS ADM. Contributed reagents/materials/analysis tools: CDB JSY. Wrote the paper: SS ADM CDB JSY.
- 1. Wise RA (2002) Brain reward circuitry: insights from unsensed incentives. Neuron 36: 229–240.
- 2. Johnson SW, North RA (1992) Opioids excite dopamine neurons by hyperpolarization of local interneurons. J Neurosci 12: 483–488.
- 3. Gysling K, Wang RY (1983) Morphine-induced activation of A10 dopamine neurons in the rat. Brain Res 277: 119–127.
- 4. Matthews RT, German DC (1984) Electrophysiological evidence for excitation of rat ventral tegmental area dopamine neurons by morphine. Neuroscience 11: 617–625.
- 5. Leone P, Pocock D, Wise RA (1991) Morphine-dopamine interaction: ventral tegmental morphine increases nucleus accumbens dopamine release. Pharmacol Biochem Behav 39: 469–472.
- 6. Spanagel R, Herz A, Shippenberg TS (1992) Opposing tonically active endogenous opioid systems modulate the mesolimbic dopaminergic pathway. Proc Natl Acad Sci USA 89: 2046–2050.
- 7. Oakman SA, Faris PL, Kerr PE, Cozzari C, Hartman BK (1995) Distribution of pontomesencephalic cholinergic neurons projecting to substantia nigra differs significantly from those projecting to ventral tegmental area. J Neurosci 15: 5859–5869.
- 8. Blaha CD, Allan LF, Das S, Inglis WL, Latimer MP, et al. (1996) Modulation of dopamine efflux in the nucleus accumbens after cholinergic stimulation of the ventral tegmental area in intact, pedunculopontine tegmental nucleus-lesioned, and laterodorsal tegmental nucleus-lesioned rats. J Neurosci 16: 714–722.
- 9. Forster GL, Falcon AJ, Miller AD, Heruc GA, Blaha CD (2002) Effects of laterodorsal tegmentum excitotoxic lesions on behavioral and dopamine responses evoked by morphine and d-amphetamine. Neuroscience 114: 817–823.
- 10. Miller AD, Forster GL, Metcalf KM, Blaha CD (2002) Excitotoxic lesions of the pedunculopontine differentially mediate morphine- and d-amphetamine-evoked striatal dopamine efflux and behaviors. Neuroscience 111: 351–362.
- 11. Miller AD, Forster GL, Yeomans JS, Blaha CD (2005) Midbrain muscarinic receptors modulate morphine-induced accumbal and striatal dopamine efflux in the rat. Neuroscience 136: 531–538.
- 12. Bechara A, Harrington F, Nader K, van der Kooy D (1992) Neurobiology of motivation: Double dissociation of two motivational mechanisms mediating opiate reward in drug-naive versus drug-dependent animals. Behav Neurosci 106: 798–807. Eur J Neurosci 21:1837–1846.
- 13. Olmstead MC, Munn EM, Franklin KBJ, Wise RA (1998) Effects of pedunculopontine tegmental nucleus lesions on responding for intravenous heroin under different schedules of reinforcement. J Neurosci 18: 5035–5044.
- 14. Rezayof A, Nazari-Serenjeh F, Zarrindast MR, Sepehri H, Delphi L (2007) Morphine-induced place preference: Involvement of cholinergic receptors of the ventral tegmental area. Eur J Pharmacol 562: 92–102.
- 15. Vilaro MT, Palacios JM, Mengod G (1990) Localization of M5 muscarinic receptor mRNA in rat brain examined by in situ hybridization histochemistry. Neurosci Lett 114: 154–159.
- 16. Weiner DM, Levey AI, Brann MR (1990) Expression of muscarinic acetylcholine and dopamine receptor mRNAs in rat basal ganglia. Proc Natl Acad Sci USA 87: 7050–7054.
- 17. Forster GL, Blaha CD (2000) Laterodorsal tegmental stimulation elicits dopamine efflux in the rat nucleus accumbens by activation of acetylcholine and glutamate receptors in the ventral tegmental area. Eur J Neurosci 12: 3596–3604.
- 18. Forster GL, Blaha CD (2003) Pedunculopontine tegmental stimulation evokes striatal dopamine efflux by activation of acetylcholine and glutamate receptors in the midbrain and pons of the rat. Eur J Neurosci 17: 751–762.
- 19. Forster GL, Yeomans JS, Takeuchi J, Blaha CD (2001) M5 muscarinic receptors are required for prolonged accumbal dopamine release after electrical stimulation of the pons in mice. J Neurosci 22: RC190.
- 20. Basile AS, Fedorova I, Zapata A, Liu X, Shippenberg T, et al. (2002) Deletion of the M5 muscarinic acetylcholine receptor attenuates morphine reinforcement and withdrawal but not morphine analgesia. Proc Natl Acad Sci USA 99: 11452–11457.
- 21. Steidl S, Yeomans JS (2008) M5 muscarinic receptor knockout mice show reduced morphine-induced locomotion, but increased locomotion following cholinergic antagonism in the ventral tegmental area. J Pharmacol Exp Ther 328: 1–13.
- 22. Takakusaki K, Shiroyama T, Kitai ST (1997) Two types of cholinergic neurons in the rat tegmental peduculopontine nucleus: electrophysiological and morphological characterization. Neuroscience 79: 1089–1109.
- 23. Yeomans JS, Maidment NT, Bunney BS (1988) Excitability properties of medial forebrain bundle axons of A9 and A10 dopamine cells. Brain Res 450: 86–93.
- 24. Ferrari-DiLeo G, Waelbroeck M, Mash DC, Flynn DD (1994) Selective labeling and localization of the M4 (m4) muscarinic receptor subtype. Mol Pharmacol 46: 1028–1035.
- 25. Takeuchi J, Fulton J, Jia ZP, Abramov-Newerly W, Jamot L, et al. (2002) Increased drinking in mutant mice with truncated M5 muscarinic receptor genes. Pharmacol Biochem Behav 72: 117–123.
- 26. Blaha CD, Winn P (1993) Modulation of dopamine efflux in the striatum following cholinergic stimulation of the substantia nigra in intact and pedunculopontine tegmental nucleus-lesioned rats. J Neurosci 13: 1035–1044.
- 27. Miller AD, Blaha CD (2005) Midbrain muscarinic receptor mechanisms underlying regulation of mesoaccumbens and nigrostriatal dopaminergic transmission in the rat.
- 28. Narita M, Nagumo Y, Hashimoto S, Narita M, Khotib J, et al. (2006) Direct involvement of orexinergic systems in the activation of the mesolimbic dopamine pathway and related behaviors induced by morphine. J Neurosci 26: 398–405.
- 29. David V, Cazala P (1994) A comparative study of self-administration of morphine into the amygdala and the ventral tegmental area in mice. Behav Brain Res 65: 205–211.
- 30. Laviolette SR, Gallegos RA, Henriksen SJ, van der Kooy D (2004) Opiate state controls bi-directional reward signaling via GABAA receptors in the ventral tegmental area. Nat Neurosci 7: 160–169.
- 31. Kohlmeier KA, Kristiansen U (2010) GABAergic actions on cholinergic laterodorsal tegmental neurons: implications for control of behavioral state. Neurosci 171: 812–829.
- 32. Jhou TC, Geisler S, Marinelli M, Degarmo BA, Zahm DS (2009) The mesopontine rostromedial tegmental nucleus: A structure targeted by the lateral habenula that projects to the ventral tegmental area of Tsai and the substantia nigra compacta. J Comp Neurol 513: 566–596.
- 33. Lecca S, Melis M, Luchicchi A, Ennas MG, Castelli MP, et al. (2011) Effects of drugs of abuse on putative rostromedial tegmental neurons, inhibitory afferents to midbrain dopamine cells. Neuropsychopharmacology 36: 589–602.
- 34. Yeomans JS, Forster G, Blaha CD (2001) M5 muscrinic receptors are needed for slow activation of dopamine neurons and for rewarding brain stimulation. Life Sci 68: 2449–2456.
- 35. Blaha CD, Jung ME (1991) Electrochemical evaluation of stearate-modified graphite paste electrodes: selective detection of dopamine is maintained after exposure to brain tissue. J Electroanal Chem 310: 317–334.
- 36. Blaha CD, Phillips AG (1996) A critical assessment of electrochemical procedures applied to the measurement of dopamine and its metabolites during drug-induced and species-typical behaviours. Behav Pharmacol 7: 675–708.
- 37. Steriade M, Datta S, Pare D, Oakson G, Curro Dossi R (1990) Neuronal activities in brain-stem cholinergic nuclei related to tonic activation processes in thalamocortical systems. J Neurosci 10: 2541–2559.
- 38. Paxinos G, Franklin KBJ (2004) The mouse brain in stereotaxic coordinates. San Diego, CA: Academic Press.