Notch signalling regulates neuronal differentiation in the vertebrate nervous system. In addition to a widespread function in maintaining neural progenitors, Notch signalling has also been involved in specific neuronal fate decisions. These functions are likely mediated by distinct Notch ligands, which show restricted expression patterns in the developing nervous system. Two ligands, in particular, are expressed in non-overlapping complementary domains of the embryonic spinal cord, with Jag1 being restricted to the V1 and dI6 progenitor domains, while Dll1 is expressed in the remaining domains. However, the specific contribution of different ligands to regulate neurogenesis in vertebrate embryos is still poorly understood.
In this work, we investigated the role of Jag1 and Dll1 during spinal cord neurogenesis, using conditional knockout mice where the two genes are deleted in the neuroepithelium, singly or in combination. Our analysis showed that Jag1 deletion leads to a modest increase in V1 interneurons, while dI6 neurogenesis was unaltered. This mild Jag1 phenotype contrasts with the strong neurogenic phenotype detected in Dll1 mutants and led us to hypothesize that neighbouring Dll1-expressing cells signal to V1 and dI6 progenitors and restore neurogenesis in the absence of Jag1. Analysis of double Dll1;Jag1 mutant embryos revealed a stronger increase in V1-derived interneurons and overproduction of dI6 interneurons. In the presence of a functional Dll1 allele, V1 neurogenesis is restored to the levels detected in single Jag1 mutants, while dI6 neurogenesis returns to normal, thereby confirming that Dll1-mediated signalling compensates for Jag1 deletion in V1 and dI6 domains.
Our results reveal that Dll1 and Jag1 are functionally equivalent in controlling the rate of neurogenesis within their expression domains. However, Jag1 can only activate Notch signalling within the V1 and dI6 domains, whereas Dll1 can signal to neural progenitors both inside and outside its domains of expression.
Citation: Ramos C, Rocha S, Gaspar C, Henrique D (2010) Two Notch Ligands, Dll1 and Jag1, Are Differently Restricted in Their Range of Action to Control Neurogenesis in the Mammalian Spinal Cord. PLoS ONE 5(11): e15515. https://doi.org/10.1371/journal.pone.0015515
Editor: Jennifer V. Schmidt, University of Illinois at Chicago, United States of America
Received: July 28, 2010; Accepted: October 10, 2010; Published: November 24, 2010
Copyright: © 2010 Ramos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was funded by Fundação para a Ciência e Tecnologia (www.fct.mctes.pt), project PTDC/SAU-OBD/75106/2006. Catarina Ramos, Susana Rocha and Claudia Gaspar are supported with fellowships from Fundação para a Ciência e Tecnologia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The vertebrate central nervous system is composed by a variety of neuronal and glial cell types, whose production has to follow three fundamental rules: i) to be generated in the correct proportion; ii) to migrate to the right position and iii) to be functionally distinct.
During embryonic spinal cord neurogenesis, neural progenitor cells are exposed to different concentrations of secreted TGFβ, Sonic hedgehog (Shh) and Wnt proteins that act in a graded manner to establish a pattern of progenitor identities along the dorso-ventral (DV) axis. This results in the generation of distinct neural progenitor domains in the spinal cord, each expressing specific combinations of transcription factors (TFs) from the homeodomain (HD) and basic-helix-loop-helix (bHLH) families, which confer specific identities to each progenitor population (reviewed in , ).
In the ventral spinal cord, five progenitor domains have been defined, four that give rise to different classes of ventral interneurons, named V0, V1, V2, and V3, and a domain from which all motoneurons (MN) arise. Similarly, neural progenitors in the dorsal spinal cord are organized into six domains that generate six early forming (dI1-6) and two late developing (dILA and dILB) classes of interneurons. Differentiating neurons arising from each progenitor domain express unique sets of TFs that regulate their final differentiation programs and their integration into the spinal cord circuitry. In the ventral spinal cord, for instance, V0 INs are characterized by the expression of Evx1, V1 INs express En1, V2a INs express Chx10, MNs express Hb9 and Isl1/2, and V3 cells express Sim1 .
Notch signalling is another mechanism that has been shown to be essential for appropriate neuronal production in the embryonic spinal cord, controlling the rate of neurogenesis , . Deletion of Notch1, which is exclusively expressed in the ventricular zone of the neuroepithelium where neurogenesis occurs results in a neurogenic phenotype that is characterized by premature and excessive neuronal differentiation in the spinal cord , . Two other Notch genes, Notch2 and Notch3, are also expressed in the embryonic neuroepithelium . Complete elimination of Notch activity could be achieved through the generation of mutant mice with simultaneous deletion of the three bHLH-O genes hes1, hes3 and hes5, which encode the main effectors of Notch signalling in the embryonic spinal cord , . Analysis of these triple-mutant mice showed that all neural progenitors in the spinal cord are dependent on Notch signalling to maintain their neurogenic potential. In the absence of Notch activity, progenitors enter differentiation prematurely and neurogenesis collapses due to progenitor depletion.
In addition to its essential role in progenitor maintenance, Notch signalling has also been shown to regulate specific neuronal fate decisions in the spinal cord, controlling for instance the generation of excitatory V2a and inhibitory V2b interneurons from the V2 domain , . These diverse Notch functions are likely mediated by different Notch ligands, all of which are expressed in the embryonic vertebrate spinal cord in unique spatio-temporal patterns. The Dll3 and Jag2 genes are expressed in differentiating neurons , , with Jag2 being expressed exclusively in differentiating motoneurons . The other ligands are specifically expressed in the ventricular region of the neuroepithelium: Dll1 and Jag1 are expressed in a strikingly complementary pattern , , with Jag1 expression restricted to the V1 and dI6 progenitor domains – and Dll1 expression present in the remaining DV progenitor domains of the embryonic spinal cord, coinciding with Dll4 in the V2 domain , .
We have previously shown that Dll1 inactivation leads to premature neuronal differentiation in all domains where the gene is expressed . Similarly, it has been recently reported that Jag1 mutants reveal accelerated neurogenesis within its domains of expression, resulting in the overproduction of V1-derived interneurons . The finding that two ligands share a common role in progenitor maintenance in adjacent domains of the embryonic spinal cord raises the question of whether one ligand could compensate for the absence of the other in regulating neuronal production. A functional equivalence between different Notch ligands has been reported in the Drosophila embryo, where complete phenocopy of Notch mutations in wing veins and sensory lineages can only be achieved after deletion of both Delta and Serrate . In addition, ectopic expression of Serrate was shown to partially rescue the severe neuronal hyperplasia observed in Delta-deficient embryos , reinforcing the notion of functional redundancy between different ligands. This is further supported by our analysis of mouse Dll1 mutants, where Dll4 can partially compensate lack of Dll1 in the spinal cord V2 domain, attenuating the overproduction of V2 INs due to Dll1 deletion .
To investigate whether Jag1 and Dll1 have differential roles in the control of neuronal production, we have used conditional mouse models to delete one or both genes specifically in the progenitor domains of the embryonic spinal cord. Analysis of neuronal production in these mutants supports a model where both ligands regulate neurogenesis in similar ways within their own domains of expression. However, Dll1 and Jag1 show different signalling capacities to adjacent domains: while Dll1 is able to signal to Jag1-expressing domains, regulating neuronal production in the absence of Jag1, the latter is unable to sustain neurogenesis in adjacent Dll1-expressing domains, when Dll1 is inactivated. Thus, Dll1 is able to compensate for the loss of Jag1 function, while Jag1 fails to do the same in the absence of Dll1.
Materials and Methods
Animal experiments were approved by the Animal Ethics Committee of Instituto de Medicina Molecular (AEC_027_2010_DH_Rdt_general_IMM) and according to National Regulations.
Mouse Strains and Sample Collection
Nestin-Cre  and Rosa26-YFP  strains were a kind gift from Rüdiger Klein (Max Planck Institute, Munich, Germany) and Nicoletta Kessaris (Wolfson Institute for Biomedical Research, London, UK), respectively. Floxed Dll1  and floxed Jag1  mice were kindly provided by Julian Lewis (Cancer Research UK, London, UK).
Mice carrying the conditional floxed Dll1 allele (Dll1f/f) or the floxed Jag1 allele (Jag1f/f) were crossed with Nestin-Cre mice (NesCre) and double heterozygous progeny was identified by PCR. Double heterozygous mice were crossed with mice homozygous for the conditional allele, to produce litters containing conditional single knockout mice (Dll1f/f;NesCre or Jag1f/f;NesCre) and littermate controls. While Dll1f/f;NesCre embryos are embryonic lethal, Jag1f/f;NesCre mice are viable and fertile.
To obtain double mutant embryos, with different allelic doses of Dll1 and Jag1, double floxed mice (Dll1f/f;Jag1f/f) were crossed to triple heterozygous mice (Dll1f/+;Jag1f/+;NesCre). From these crosses, we obtained embryos with the following genotypes: Dll1f/f;Jag1f/f;NesCre/Dll1f/f;Jag1f/+;NesCre/Dll1f/+;Jag1f/f;NesCre/Dll1f/+;Jag1f/+;NesCre. Embryos were collected at E11.5, 13.5 and 15.5.
To identify cells where Cre-mediated recombination occurred, Jag1f/f mice were made homozygous for the Rosa26-YFP transgene (Jag1f/f;Rosa26YFP/YFP). The progeny was crossed with Jag1f/f;NesCre mice to generate Jag1f/f;Rosa26YFP/+;NesCre and control littermates.
All animals were fed ad-libitum and housed in SPF facilities.
Immunofluorescence and in situ hybridization
Embryos were fixed in 4% paraformaldehyde at 4°C (2 h for immunofluorescence (IF) and O/N for in situ hybridization (ISH)), cryoprotected in 30% sucrose and embedded in 7.5% gelatin:15% sucrose and 12 µm sections were used in the analysis.
For IF, sections were degelatinized at 37°C for 15 min, followed by a pre-treatment with 3%H2O2: Methanol for 30 min at room temperature (RT), except for the antibodies against Jag1 and GFP.
Permeabilization was performed using Triton ×100 (0.5%) for 15 min, followed by blocking (10% Normal Goat Serum, 0.1% Triton ×100) for 1 h at RT. Primary antibodies were incubated O/N at 4°C. The following antibodies were used in this study: rabbit anti-Bhlhb5 (1∶10000; kind gift of Michael Greenberg), mouse anti-Calbindin (1∶500, Swant), sheep anti-Chx10 (1∶100, Exalpha), rabbit anti-En1 (1∶100, kind gift of Alex Joyner), mouse anti-Evx1 (1∶100, Developmental Studies Hybridoma Bank), rabbit anti-Foxd3 (1∶25, kind gift of Thomas Muller), rabbit anti-Foxp2 (1∶200, Abcam), rat anti-GFP (1∶1000, Nacalai Tesque), mouse anti-Islet1 (1∶1000; Developmental Studies Hybridoma Bank), rabbit anti-Jag1 (1∶50, Santa Cruz) and rabbit anti-Pax2 (1∶200, Covance). Sections were subsequently washed and incubated with appropriate Alexa Fluor (488 or 594)-conjugated secondary antibodies (1∶400, Molecular Probes) for 1 h at RT.
Double in situ hybridizations using Dll1 and Hes5 mRNA probes were performed as previously described , with modifications. Dll1 DIG-labelled probe was first detected with AP-conjugated anti-DIG antibody (1∶2000; Roche) and signal was developed using Fast-Red substract (Roche). To detect the second Hes5 Fluorescein-labelled probe, sections were incubated with HRP-conjugated anti-Fluorescein antibody (1∶1000, Roche), and signal developed by TSA-Plus Fluorescein System (Perkin-Elmer), according to manufacturer's instructions.
Cell counts and Imaging
Cell counts were performed for eight cryostat sections from at least three spinal cords (i.e. twenty four sections for each genotype). For the described antibodies, quantification of neuronal types was done by counting the number of immunopositive cells, which were normalized to the total number of cells (DAPI) in images taken with either a 20× or 40× objective on a Leica DM5000B fluorescence microscope. Statistical significance was determined using Student's t- test. Confocal images were captured with Zeiss LSM510 META confocal microscope.
Jag1 mutants exhibit a milder neurogenic phenotype than Dll1 mutants
To investigate the role of Jag1 and Dll1 in regulating neuronal production within and outside their domains of expression in the embryonic spinal cord, we have analysed in parallel the phenotypes of mutant embryos where either Jag1 or Dll1 were specifically inactivated in the neuroepithelium. These embryos were obtained by crossing floxed Jag1 and floxed Dll1 mice ,  with mice carrying a Cre recombinase under the control of the rat Nestin promoter, which drives Cre expression in all neural progenitors . Jag1 single mutant (Jag1f/f;NesCre) and Dll1 single mutant embryos (Dll1f/f;NesCre) were compared between them and with control littermates.
Comparison of Jag1f/f;NesCre with control embryos at E10.5 and E11.5 showed no differences in the general morphology of the spinal cord, whereas E11.5 Dll1f/f;NesCre spinal cords were severely affected as an enlargement of the floor plate, accompanied by the disappearance of the central lumen could be observed . A similar morphology has been reported in a conditional Notch1 mutant .
In order to monitor the production of the distinct INs arising from the Jag1-expressing V1 and dI6 domains of the embryonic spinal cord, as well as the Dll1-expressing V2 and V0 domains, we have used various markers, individually or in combinations, as depicted in Figure 1. For V1-derived interneurons (INs), we followed the expression of En1, a homeobox- containing TF, and Foxd3, a winged-helix TF, which are both expressed in all post-mitotic V1 INs , . To detect specific subsets of V1-derived neurons at later stages, we used Calbindin expression to label Renshaw cells ,  and Foxp2 expression to mark non-Renshaw cells . To identify dI6 INs, we have analysed the expression of bHLHb5, a TF present in dI6 INs and also in more ventral V1 and V2 INs, but not in V0 INs . Combined analysis with Evx1, which is selectively expressed by a more ventral subset of V0 INs (V0v) , , allows the unequivocal identification of dI6 INs. In addition, we have evaluated the expression of Pax2, a TF common to multiple spinal cord INs, including the dI6 INs, as well as the V0 and V1 INs, but not to dI5 INs . Finally, the expression of the homeodomain TF Chx10 was used to identify V2a INs arising from the Dll1-expressing V2 domain .
The mutually exclusive expression pattern of Jag1 and Dll1 in relation to the transcription factor code used to identify different types of INs arising from each progenitor domain. At E11.5, V1 INs are identified through the expression of Foxd3 and En1 , . A subpopulation of V2 INs (the excitatory V2a INs) expresses Chx10 and a subpopulation of V0 INs (the V0V) expresses Evx1 , . dI6 INs were characterized through the combined expression of Pax2, Bhlhb5 and Evx1. Pax2 is a TF common to multiple INs, such as dI6, V0 and V1 INs but not to V2 or dI5 INs. Pax2+ cells dorsally located to Evx1+ V0V INs and ventrally to Pax2− dI5 INs, are either dI6 or Evx1− V0D INs. To detect only dI6 INs, we analysed the expression of Bhlhb5 combined with Evx1. All Bhlhb5+ INs, located dorsally to Evx1+ V0V INs, are dI6 INs .
Quantification of Foxd3+ V1 INs in Jag1f/f;NesCre embryonic spinal cord at E11.5 revealed that lack of Jag1 function results in a mild, but statistically significant, increase of V1 IN production, when compared to control embryos (Fig. 2 A,C,D). On the contrary, Dll1f/f;NesCre embryos showed similar numbers of Foxd3+ V1 INs to that in control embryos (Fig. 2 A,B,D). This Jag1-specific V1 phenotype was further confirmed by a modest increase of En1+ INs found in Jag1 mutants at E11.5, when compared to control embryos (Fig. S1). A recently published work has also reported an increase in V1 INs on a different Jag1 mutant mouse , although the V1 neurogenic phenotype we observed in Jag1f/f;NesCre embryos is not as striking.
(A–C) Inactivation of Dll1 does not alter Foxd3+ V1 neurogenesis but results in Chx10+ V2a INs overproduction. Inactivation of Jag1 leads to a modest increase in Foxd3+ V1 INs but Chx10+ V2a neurogenesis remains unaffected. Scale bar 100 µm. (D, E) Cell counts of Foxd3+ cells at E11.5 show a 23% increase of V1 INs in Jag1 mutants, while quantification of Chx10+ V2a INs shows an increase of 51% when compared to control littermates. Error bars represent s.d. for biological triplicates. Student's t-test: *p<0.05; **p<0.005.
We next quantified the number of Chx10+ V2a INs in Jag1f/f;NesCre embryos (Fig. 2 C,E), and no significant alteration was observed, in contrast with the marked increase of V2a INs detected in Dll1f/f;NesCre mutants, (Fig. 2 B,E). Noticeably, the increase of V1 INs in Jag1f/f;NesCre mutants is less pronounced than the increase in V2a INs found in Dll1f/f;NesCre mutants, being also statistically less significant (t-test, p<0.05 versus p<0.005) (Fig. 2 D, E). Our findings show that Jag1 is necessary to maintain the normal pace of neurogenesis within the V1 domain, but is not controlling progenitor maintenance in the adjacent Dll1-expressing V2 domain. In addition, the relatively mild Jag1 phenotype in the V1 domain suggests that not all V1 neural progenitors are affected by the lack of Jag1-mediated Notch signalling. This is further supported by our finding that the number of later V1-derived IN sub-types (Calbindin+ and Foxp2+) is not altered in Jag1f/f;NesCre spinal cords (E15.5), when compared to control embryos (Fig. S2). Together, these results raise the hypothesis that control of V1 neurogenesis in the absence of Jag1 may be, at least partially, rescued by Dll1 signalling from the V0 and V2 neighbouring domains.
To further test this hypothesis, we analysed neuronal production in the other Jag1-expressing domain of the spinal cord, the dI6 domain. Our results show that the number of Bhlhb5+ dI6 INs in Jag1f/f;NesCre (E11.5) embryos is indistinguishable from that detected in control and in Dll1f/f;NesCre embryos (Fig. 3 A–C,G). Similarly, quantification with Pax2 confirmed that dI6 neurogenesis is not affected in Jag1 mutants, when compared to control (Fig. 3 D,F,H). The normal production of dI6 INs in Jag1 mutants offers further support to the hypothesis that Dll1 signalling from adjacent domains can compensate the absence of Jag1 and restore the control of neurogenesis. The increase in the number of Pax2+ INs detected in Dll1 mutants (Fig. 3 E,H) results from the overproduction of Pax2+/Evx1− V0D INs, and is not due to an excess of Pax2+/Bhlhb5+ dI6 INs (Fig. 3 D–F, H). In parallel, we confirmed that Dll1 is necessary and sufficient for the control of V0 neurogenesis, as an increase of Evx1+ V0V INs could only be detected in Dll1, and not in Jag1, mutants (Fig. 3 A–F, I).
(A–F and I) Dll1 mutants show a 78% increase of Evx1+ V0V INs (indicated with asterisk), whereas V0 neurogenesis in Jag1 mutants is indistinguishable from that of control littermates. Scale bar 50 µm. Student's t-test: ** p<0.005. (A–C and G) Inactivation of either Dll1 or Jag1 does not alter Bhlhb5+ dI6 INs neurogenesis. Quantification was restricted to Bhlhb5+ INs, dorsally located to Evx1+ V0V INs. Scale bar 50 µm. (D–F and H) When compared to control embryos, Dll1 mutants show a 38% increase in Pax2+ dI6 and V0D INs, located between Evx1+ V0V INs and Pax2− dI5 INs. This increase is due to Pax2+/Bhlhb5−/Evx1− V0D INs overproduction and not an increase in Pax2+/Bhlhb5+/Evx1− dI6 neurogenesis. Number of Pax2+ dI6 and V0D INs is similar in Jag1 mutants and control littermates. Error bars represent s.d. for biological triplicates. Student's t-test: * p<0.05.
Nestin-Cre driver effectively inactivates Jag1 in V1 and dI6 spinal cord progenitors
To exclude that the mild neurogenic phenotype found in Jag1f/f;NesCre embryos was due to poor Cre recombinase activity driven by the Nestin-Cre driver, we evaluated the extent of Nestin Cre-mediated recombination in the embryonic spinal cord of Jag1 mutants. To assess this, a Rosa26-derived reporter line that conditionally expresses the YFP gene (Rosa26-YFP) was bred into the Jag1f/f;NesCre line, allowing us to identify cells where Cre-mediated recombination is active . E11.5 Jag1f/f;R26-YFP/+;NesCre embryos were collected and exhibited an intense YFP immunofluorescence along the whole DV axis of the developing spinal cord, indicating widespread Cre-mediated recombination in the neuroepithelium (Fig. 4 A,B and A″,B″). In addition, we have used immunofluorescence to detect the presence of the Jag1 protein in control and Jag1 mutant embryos. Our results show that Jag1 is completely absent from the dI6 and V1 domains of Jag1 mutants, demonstrating that the Nestin-Cre driver effectively deletes Jag1 in the embryonic spinal cord (Fig. 4 A′,B′ and A″,B″).
(A, B) Immunofluorescence in Jag1f/f;Rosa26YFP/+;NesCre E11.5 embryos shows widespread YFP expression, indicating that most of the cells had undergone Cre-mediated recombination. Since YFP signal fades away during longer fixations, IF with an anti-GFP antibody was used to detect YFP expression. (A′, B′) The two characteristic stripes of Jag1-protein expression are absent in Jag1f/f;Rosa26YFP/+;NesCre embryos. (A″, B″) Merge of YFP and Jag1 expression. Scale bar 50 µm. Strong red signal in B′, B″ is due to erythrocytes.
Notch signalling is still active in the V1 domain of Jag1 mutants
Given the mild V1 phenotype detected in Jag1 mutant embryos, we next asked whether Notch signalling continues to be active in the V1 domain, even in the complete absence of Jag1 protein in the mutant neuroepithelium. To address this, we analysed the expression of Hes5, the main target and effector of Notch activity in the developing spinal cord . In situ hybridization with a Hes5 probe in Jag1f/f;NesCre and control embryos revealed that Hes5 mRNA expression is slightly diminished in the V1 domain of Jag1 mutants, but is still broadly detected in V1 progenitors (Fig. 5 A,B). Simultaneous detection of Dll1 mRNA expression shows that Dll1 transcription continues to be excluded from the V1 domain of Jag1f/f;NesCre embryos (Fig. 5). These findings confirm the absence of cross-inhibition between the two genes in the developing spinal cord, as previously suggested by studies in the chick embryo, where missexpression of Dll1 or Jag1 did not alter the endogenous expression domains of Jag1 and Dll1, respectively .
(A, B) At E11.5, expression of Hes5 mRNA can still be detected in the V1 domain (between dashed white lines) of Jag1 mutants, although levels seem reduced when compared to control spinal cords. (A′, B′) The gap of Dll1 mRNA expression, corresponding to Jag1+ V1 domain (between dashed white lines), is present in both control and Jag1 mutants. (A″, B″) Merge of double in situ hybridization for Dll1 and Hes5 showing that Hes5 mRNA is still present within Dll1− V1 cells of Jag1 mutants. Scale bar 20 µm. (C, D) High-resolution confocal images of double in situ hybridization for Dll1 and Hes5 confirm that inactivation of Jag1 does not abolish Notch signalling in the V1 domain (between dashed white lines). Images show the presence of Dll1+ cells in the V0 (dorsally to white dashed lines) and V2 (ventrally to white dashed lines) domains flanking Hes5+ V1 progenitors in the neuroepithelium of both control and Jag1 mutants. Scale bar 20 µm.
The observed Notch activity in the V1 domain of Jag1 mutant embryos favours the hypothesis that Dll1-expressing cells located at the boundary between the V0/V1 and V1/V2 domains are capable of signalling to neural progenitors in the adjacent V1 domain, preventing massive differentiation of V1 INs. Consistent with this observation, high-resolution confocal analysis of the spinal cord neuroepithelium in Jag1f/f;NesCre and control embryos after Dll1/Hes5 double in situ hybridization shows the presence of Dll1-expressing cells flanking Hes5+ V1 progenitors, suggesting that cells from neighbouring domains are indeed able to laterally signal to V1 progenitors and mediate Notch-driven Hes5 expression in these cells (Fig. 5 C,D).
Dll1-mediated signalling from adjacent domains can compensate absence of Jag1 in V1 and dI6 domains
To definitively confirm our hypothesis that Jag1 absence is compensated by Dll1 from adjacent domains, we generated mutant embryos where both Dll1 and Jag1 were conditionally deleted in the neuroepithelium. For this purpose, we crossed double-floxed Dll1;Jag1 female mice (Dll1f/f;Jag1f/f) with males carrying one floxed allele of Dll1, one floxed allele of Jag1 and one allele of the Nestin-Cre driver (Dll1f/+;Jag1f/+;NesCre). This strategy allowed us to generate an allelic series for phenotypic analysis. Neuronal production was monitored in these embryos using the previously described markers (Fig. 1). For all neuronal types assessed, double heterozygote embryos (Dll1f/+;Jag1f/+;NesCre) were indistinguishable from control embryos and were therefore used as controls (data not shown).
If Dll1 signalling from adjacent domains is able to control neurogenesis in the V1 domain of Jag1 mutants, the prediction is that the mild V1 phenotype detected in Jag1f/f;NesCre embryos would become more pronounced in the absence of the two ligands. Indeed, quantification of Foxd3+ V1 INs in E11.5 full conditional Dll1;Jag1 double mutants (Dll1f/f;Jag1f/f;NesCre) revealed the highest increase, when compared to all other genotypes. For instance, single Jag1 mutants displayed a 23% increase in Foxd3+ V1 INs (p<0.05), while Dll1f/f;Jag1f/f;NesCre mutants exhibited a 49% increase (p<0.005) (Fig. 6 A,J). This excess in V1 neurogenesis was further confirmed by the analysis of En1+ V1 INs in Dll1f/f;Jag1f/f;NesCre mutants (Fig. S3). In addition, Dll1f/f;Jag1f/f;NesCre mutants showed a marked increase in V1-derived Calbindin+ Renshaw cells and FoxP2+ non-Renshaw cells (Fig. S4), in contrast to single Jag1f/f;NesCre mutant embryos (Fig. S2).
In double mutant Dll1f/f;Jag1f/f;NesCre embryos (A), both Foxd3+ V1 INs and Chx10+ V2a INs are strongly increased, while one functional copy of Dll1 (B) rescues the V2a phenotype completely and the V1 phenotype partially. On the contrary, one functional copy of Jag1, in the absence of Dll1 (C), rescues the V1 phenotype but fails to revert the excess of V2a INs. (D–I) A neurogenic phenotype in the dI6 domain can only be observed in the absence of both ligands, using either bHLHb5 (D–F) or Pax2 (G–I) to identify dI6 neurons, located dorsally to the Evx1+ V0V INs (indicated with asterisk). The presence of one functional copy of Dll1 (E,H) or of Jag1 (F,I) is enough to prevent excessive dI6 neurogenesis. The number of Evx1+ V0V INs is only increased in the complete absence of Dll1 (D,F,G,I) as one functional copy of Dll1 is enough to revert the V0V neurogenic phenotype, even in the absence of Jag1 (E,H). Although one functional copy of Jag1 (F) is enough to revert the excess of Bhlhb5+ dI6 INs detected in Dll1f/f;Jag1f/f;NesCre embryos (D), an excess of Pax2+ INs located dorsally to Evx1+ V0V INs (indicated with asterisk) can still be detected in Dll1f/f;Jag1f/+;NesCre embryos (I), when compared to Dll1f/f;Jag1f/f;NesCre (G). The excess of Pax2+ INs arises from the Dll1-dependent V0D domain (Pax2+/Evx1−) and not from the Jag1-expressing dI6 domain (Pax2+/Evx1−/Bhlhb5+). On the contrary, one functional copy of Dll1 is enough to rescue both the V0D and dI6 neurogenic phenotypes (E,H). Scale bar 50 µm. (J–N) Graphics depicting the quantification of various types of INs in different allelic combinations of Dll1 and Jag1. The percentage of positive cells for each marker is relative to the total number of cells, detected by DAPI staining of the entire spinal cord sections where the counts were done. Error bars represent s.d. for at least three biological replicates. Student's t-test * p<0.05; ** p<0.05; *** p<0.001.
Analysis of the dI6 domain shows also a clear increase in the number of Pax2+ and Bhlhb5+ dI6 INs in full double mutant embryos (Dll1f/f;Jag1f/f;NesCre) when compared to control or to single mutant Jag1f/f;NesCre embryos (Fig. 6 D,G,K).
Together, these results indicate that the absence of Jag1 activity in the V1 and dI6 domains of the developing spinal cord can be compensated by Dll1-mediated signalling from adjacent domains. This is further supported by the finding that one functional copy of Dll1 (Dll1f/+;Jag1f/f;NesCre) is sufficient to partially compensate for the lack of Jag1 in the V1 domain, reverting the stronger V1 phenotype observed in Dll1f/f;Jag1f/f;NesCre embryos into a mild phenotype, similar to that observed in Jag1 single mutants (Fig. 6 B,J). Moreover, an identical trend is detected in the dI6 domain, where the presence of one functional copy of Dll1 results in normal numbers of dI6 INs (Fig. 6 E,M). These embryos, with just one copy of Dll1, show also a full rescue of the excessive differentiation of Chx10+ V2a (Fig. 6 B,K), and of Evx1+ V0 INs (Fig. 6 E,H,L), confirming the functional activity of the Dll1 allele.
To test gene dosage dependence of the Jag1 phenotype, we analysed embryos where only one functional copy of Jag1 is present, in the complete absence of Dll1 (Dll1f/f;Jag1f/+;NesCre). In these embryos, the number of Foxd3+ V1 INs is similar to that detected in control embryos, revealing that one functional copy of Jag1 is sufficient to ensure normal control of V1 neurogenesis (Fig. 6 C,J). The same applies to the dI6 domain, where the number of Bhlhb5+ dI6 INs detected in E11.5 Dll1f/f;Jag1f/+;NesCre embryos is comparable to that found in control littermates (Fig. 6 E,I). Quantification of Pax2+ INs in Dll1f/f;Jag1f/+;NesCre embryos shows similar numbers to those in single Dll1 and Dll1f/f;Jag1f/f;NesCre mutants (Fig. 6 I,N). From these results we could confirm that the excess of Pax2+ INs located dorsally to Evx1+ is not due to the overproduction of dI6 INs, but rather of V0D INs.
The finding that one functional copy of either Jag1 or Dll1 is able to rescue the dI6 phenotype detected in Dll1f/f;Jag1f/f;NesCre embryos, together with our data showing that dI6 neurogenesis is not affected in either Dll1 or Jag1 single mutants, imply that both ligands are able to control the rate of dI6 neurogenesis.
Finally, we evaluated V2 and V0 neurogenesis in Dll1f/f;Jag1f/+;NesCre embryos at E11.5 and found that the functional copy of Jag1 present in these embryos is unable to rescue the increases in Chx10+ V2a (Fig. 6 C,K) and Evx1+ V0 INs (Fig. 6 F,I,L) due to Dll1 deletion. These results confirm that Jag1 does not signal to adjacent Dll1-expressing domains and that neurogenesis in these domains is exclusively regulated by Dll1. (A schematic summary of the above results is presented in Fig. S5).
Although Notch signalling is widely used during several developmental processes, it is not yet clear how different Notch ligands are employed to control a multitude of distinct cellular decisions. Here, we address the function of two different ligands, Dll1 and Jag1, during spinal cord neurogenesis. These ligands are expressed in non-overlapping complementary domains of the embryonic spinal cord, and analysis of mouse embryos carrying mutations in Dll1 and Jag1, singly or in combination, reveals that the two ligands play equivalent roles in controlling the rate of neuronal production within their domains of expression. However, while Jag1 signalling is restricted to cells within its domains of expression, our results reveal that Dll1 is able to signal to neural progenitors in the adjacent Jag1-expressing domains and prevent their untimely differentiation in the absence of Jag1 function. These results imply that Dll1- or Jag1-mediated activation of Notch in the spinal cord neuroepithelium is not qualitatively different, with both ligands contributing to regulate neural progenitor maintenance but not neuronal cell type diversity.
Dll1 and Jag1 are functionally equivalent in controlling the rate of neurogenesis within their expression domains
In mammals, four Notch receptors (Notch1–4) can bind five different ligands, named Delta-like (Dll) 1, 3 and 4, and Jagged (Jag) 1 and 2 . All ligands exhibit different expression patterns during embryonic spinal cord neurogenesis. While Jag1 and Dll1 are expressed transiently in non-overlapping complementary domains along the DV axis, in cells committed to differentiation , Dll3 is expressed later in differentiated neurons, across all DV domains . A more restricted expression pattern is shown by Dll4, that is exclusively expressed by V2 differentiating neurons , , and by Jag2, which is expressed in differentiated MNs .
Our previous work showed that Dll1 signalling is necessary to regulate neurogenesis and that Dll1 deletion causes a neurogenic phenotype characterized by premature and excessive neuronal differentiation in the spinal cord domains where the gene is normally expressed . A recent paper reported that deletion of Jag1 causes an acceleration of neurogenesis in the V1 domain where this gene is expressed, suggesting that Dll1 and Jag1 play similar functions within their expression domains, controlling the rate of neuronal differentiation .
Here, we have analysed a conditional Jag1 mutation in the developing spinal cord and confirmed that Jag1 is necessary in the V1 domain to regulate neurogenesis. However, when compared to the excessive neuronal differentiation caused by Dll1 mutation in the V0 and V2 domains, the V1 neurogenic phenotype due to Jag1 deletion is milder and seems to be rescued at later stages, as two V1-derived subtypes of INs are produced in normal numbers in Jag1 mutants. This milder phenotype correlates with our finding that Notch activity is still present in the V1 domain of Jag1 mutants, as detected by the expression of the Notch target and effector Hes5.
These results led us to consider the hypothesis that deletion of Jag1 in the V1 and dI6 domains can be compensated by Dll1 signalling from adjacent domains. Since there is no evidence for any physical boundary separating the various progenitor domains along the D-V axis of the embryonic spinal cord, it is conceivable that Dll1-expressing cells, in direct contact with V1 and dI6 progenitors, may activate Notch in these cells, enabling neurogenesis to proceed at normal pace in the absence of Jag1. Neuroepithelial cells expressing Dll1 might even reach progenitors located further away inside the V1 and dI6 domains, as suggested by the recent findings that the Drosophila Delta protein is present in filopodi of signalling cells within the fly wing and notum epithelium, being able to mediate lateral inhibition over several cell diameters during specification of sensory organ precursors , .
Given the difference in width of the two Jag1-expressing domains, with the V1 domain being 2–3 times wider than the dI6 domain (see Fig. 3), the predicted long-range signalling ability of Dll1-expressing cells could account for our findings that dI6 neurogenesis is normal in Jag1-mutant embryos and that only a milder neurogenic phenotype could be detected in the V1 domain. In this scenario, neural progenitors at the centre of the wider V1 domain may be too far to be reached by neighbouring Dll1-expressing cells, and will commit to differentiation in the absence of Jag1 signalling, while all progenitors in the thinner dI6 domain receive Dll1-mediated signalling.
In Jag1-expressing domains, control of neurogenesis can be achieved by either Jag1- or Dll1-mediated Notch signalling
To test whether Jag1 inactivation can be compensated by Dll1-signalling from adjacent domains, we have generated an allelic series of Dll1;Jag1 double mutants and analysed neuronal production in the spinal cord of the various mutant combinations. Our results show that simultaneous deletion of both copies of Dll1 and of Jag1 causes an extensive differentiation of various subtypes of INs produced from the DV domains where each ligand is expressed. In the V1 domain, we could observe that absence of both Jag1 and Dll1 causes a stronger and more significant increase in the number of INs than that observed in Jag1 single mutants. In the case of the dI6 domain, a neurogenic phenotype can only be detected when both Jag1 and Dll1 are deleted. Thus, clear disruption of V1 and dI6 neurogenesis only occurs when the two ligands are deleted, showing that Dll1-signalling is indeed able to compensate for lack of Jag1. This conclusion is further supported by the finding that a single copy of Dll1 (in Dll1f/+;Jag1f/f;NesCre embryos) is enough to restore dI6 neurogenesis and revert the strong V1 neurogenic phenotype to a milder one, similar to that detected in Jag1 single mutants. In addition, the fact that the identity of dI6 and V1 INs is not altered when Jag1-mediated signalling is replaced by Dll1-mediated signalling from adjacent domains reveals that these Notch ligands do not regulate neuronal type specification within each DV domain.
Dll1 and Jag1 are differently restricted in their range of action to control neurogenesis in the developing spinal cord
While our results show that Dll1 can signal outside its own domains of expression and compensate for the absence of Jag1 in the dI6 and V1 domains, Jag1 can only control neurogenesis inside these domains, failing to compensate Dll1 deletion in adjacent domains. This is particularly evident in our analysis of dI6 neurogenesis: while double mutant embryos (Dll1f/f; Jag1f/f; NesCre embryos) show a marked increase in Pax2+ INs derived from the neighbouring dI6 and V0 domains, the presence of one functional copy of Jag1 (Dll1f/f;Jag1f/+;NesCre) is able to restore the normal number of dI6 INs (identified as Pax2+/Bhlhb5+) but not the number of the immediately adjacent dorsal V0D INs (also Pax2+ but negative for bHLHb5 and Evx1).
The described incapacity of Jag1 to signal to neighbouring cells within Dll1-expressing domains might be due to the presence of Lunatic Fringe (LFng), which is known to modulate the response of Notch receptors to different ligands –. Actually, LFng is expressed in the same domains as Dll1 and is excluded from the dI6 and V1 domains, where Jag1 is expressed . Studies in both Drosophila and vertebrates have shown that the o-fucosyltransferase activity of Fng proteins leads to a modification in Notch receptors that blocks activation of the pathway by the Serrate/Jagged class of ligands . This offers a simple explanation for the finding that Jag1 is unable to compensate the absence of Dll1 in neighbouring progenitors, as Notch receptors in these cells have been modified by LFng and are therefore unable to be activated by Jag1.
On the contrary, several reports have shown that modification of Notch by Fringe enhances Delta-mediated activation , , . This suggests that the overlapping LFng and Dll1 expression in the developing spinal cord results in high levels of Notch activity, which are necessary for the proper control of neurogenesis. However, our results indicate that Fringe activity is not absolutely needed for the ability of Notch to respond to Dll1-signalling during neurogenesis: in the absence of Jag1, a functional copy of Dll1 is sufficient to regulate neural progenitor differentiation in the Fringe-negative dI6 and V1 domains, thereby implying that the levels of Notch activity elicited by Dll1 binding are still sufficient to control neurogenesis. This is in agreement with biochemical data reported by Yang et al., who showed that, in the absence of Fringe, the levels of Notch activity elicited by Dll1 or Jag1 are identical . These findings also suggest that levels of Notch activity are not uniform along the DV axis of the developing spinal cord, being higher in Dll1+/LFng+ domains than in Jag1+/LFng− domains. Nonetheless, our results do not support the model proposed by Marklund et al., in which both Dll1 and Jag1 are prohibited from signalling across domain boundaries . This model is based on the finding that ectopic Dll1 expression in the chick spinal cord was unable to inhibit neuronal differentiation in the Jag1-expressing V1 domain. However, this data does not rule out that Dll1-signalling from cells located in adjacent domains can activate Notch in V1 and dI6 progenitors, as the endogenous expression of Jag1 in electroporated cells can result in cis-inhibition of the ectopically expressed Dll1. A similar cis-inhibition of Dll4 signalling by Jag1 has been described in stalk cells during retina angiogenesis  and might explain the lack of Dll1 activity in the chick gain-of-function experiments .
In summary, Dll1 and Jag1 can similarly activate Notch signalling in neural progenitors of the embryonic spinal cord to regulate their commitment to differentiation, although the two ligands are differently restricted in their range of action: while Jag1 is effectively prevented from signalling to progenitors located in adjacent Dll1-expressing domains, Dll1 can efficiently signal to progenitors in Jag1-expressing domains and regulate their differentiation.
Inactivation of Jag1 leads to a modest increase of En1+ V1 INs. (A, B) Immunofluorescence analysis of MNs (Islet1+) and V1 INs (En1+) in control and Jag1 mutants at E11.5 shows that inactivation of Jag1 does not alter MN neurogenesis and confirms the modest increase in the production of V1 INs. Scale bar 50 µm.
Generation of later V1-derived neuron subtypes is not affected in Jag1 mutants. At E15.5, generation of Foxp2+ non-Renshaw cells (A, B), and Calbindin+ Renshaw cells (C, D) is similar in control and Jag1 mutant spinal cords. Scale bar 100 µm.
Simultaneous inactivation of Dll1 and Jag1 results in a marked increase of En1+ V1 INs. (A, B) Immunofluorescence analysis of V1 INs (En1+) in control and Dll1f/f;Jag1f/f;NesCre embryos at E11.5 showing that inactivation of both ligands leads to a marked overproduction of V1 INs. Scale bar 100 µm.
Simultaneous inactivation of Dll1 and Jag1 results in overproduction of two later V1-derived neuron subtypes. An excess of Foxp2+ non-Renshaw cells (A, B), and of Calbindin+ Renshaw cells (C, D) is only detected in Dll1f/f;Jag1f/f;NesCre embryos, when compared to control littermates. Scale bar 100 µm.
We thank Sara Ferreira for excellent technical help, Yuri Leite for mouse handling and Elsa Abranches for critical reading of the manuscript. We are grateful to M. Greenberg, A. Joyner and T. Muller for the generous gift of antibodies. We are also thankful to J. Lewis for providing the floxed Jag1 and Dll1 mouse strains, to R. Klein for Nestin-Cre strain and to N. Kessaris for Rosa26-YFP mouse strain.
Conceived and designed the experiments: CR DH. Performed the experiments: CR CG SR. Analyzed the data: CR CG SR DH. Wrote the paper: CR DH.
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