Additional chromosomal abnormalities are currently detected in Burkitt's lymphoma. They play major roles in the progression of BL and in prognosis. The genes involved remain elusive. A whole-genome oligonucleotide array CGH analysis correlated with karyotype and FISH was performed in a set of 27 Burkitt's lymphoma-derived cell lines and primary tumors. More than half of the 145 CNAs<2 Mb were mapped to Mendelian CNVs, including GSTT1, glutathione s-transferase and BIRC6, an anti-apoptotic protein, possibly predisposing to some cancers. Somatic cell line-specific CNVs localized to the IG locus were consistently observed with the 244 K aCGH platform. Among 136 CNAs >2 Mb, gains were found in 1q (12/27), 13q (7/27), 7q (6/27), 8q(4/27), 2p (3/27), 11q (2/27) and 15q (2/27). Losses were found in 3p (5/27), 4p (4/27), 4q (4/27), 9p (4/27), 13q (4/27), 6p (3/27), 17p (3/27), 6q (2/27),11pterp13 (2/27) and 14q12q21.3 (2/27). Twenty one minimal critical regions (MCR), (range 0.04–71.36 Mb), were delineated in tumors and cell lines. Three MCRs were localized to 1q. The proximal one was mapped to 1q21.1q25.2 with a 6.3 Mb amplicon (1q21.1q21.3) harboring BCA2 and PIAS3. In the other 2 MCRs, 1q32.1 and 1q44, MDM4 and AKT3 appeared as possible drivers of these gains respectively. The 13q31.3q32.1 <89.58–96.81> MCR contained an amplicon and ABCC4 might be the driver of this amplicon. The 40 Kb 2p16.1 <60.96–61> MCR was the smallest gained MCR and specifically encompassed the REL oncogene which is already implicated in B cell lymphomas. The most frequently deleted MCR was 3p14.1 <60.43–60.53> that removed the fifth exon of FHIT. Further investigations which combined gene expression and functional studies are essential to understand the lymphomagenesis mechanism and for the development of more effective, targeted therapeutic strategies.
Citation: Toujani S, Dessen P, Ithzar N, Danglot G, Richon C, Vassetzky Y, et al. (2009) High Resolution Genome-Wide Analysis of Chromosomal Alterations in Burkitt's Lymphoma. PLoS ONE 4(9): e7089. https://doi.org/10.1371/journal.pone.0007089
Editor: Syed A. Aziz, Health Canada, Canada
Received: May 7, 2009; Accepted: August 20, 2009; Published: September 17, 2009
Copyright: © 2009 Toujani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The work was financed by the Canceropole Ile de France, the CNRS and the IGR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
First described by D. Burkitt in 1958, Burkitt's lymphoma (BL) is a monoclonal proliferation of malignant B lymphocytes. It is a mature aggressive lymphoma that accounts for 30 to 50% of lymphomas in children and for 1 to 2% in adults . Its original description, a jaw tumor, led to the discovery of Epstein-Barr virus (EBV). Because of its high frequency in children living in the African equatorial regions (the Burkitt Belt), heavily infected by EBV and malaria  this viral linked tumor was qualified of endemic. It was identical, by morphology (cytology and pathology), immunophenotype and chromosome abnormalities to mature aggressive lymphomas, mainly EBV negative, that were observed in all the world. This type of BL, coined as sporadic, accounts for 30 to 50% of lymphomas in children and for 1 to 2% in adults . The treatment of endemic and sporadic BL rely on high dose of chemotherapy with good results in children.
The hallmark of this disease is the t(8;14) translocation or one of the variant t(8;22) or t(2;8) translocations, associating the MYC gene (located at 8q24) to one of three immunoglobulin loci , . These balanced chromosomal translocations result in constitutively deregulated MYC expression by position effect, whatever the break point location and the transcription orientation , Hypothesized as a transforming factor located on 8q24, MYC was the first oncogene isolated in a human malignancy, responsible for maintaining the balance of cellular proliferation, differentiation, adhesion and apoptosis.
Additional chromosomal abnormalities, detected in 70% of pediatric BL, seem to be important factors influencing clinical outcome , . Gain of 7q and del(13q) seems strong genetic indicators of a poor prognosis . Although many of these chromosomal anomalies undoubtedly play major roles in the pathogenesis of BL, the genes involved have remained elusive.
High resolution CGH array (aCGH) ,  is a powerful method to identify pathogenic DNA copy-number changes (gain, amplification and deletion) on a genome-wide scale, and to map these changes to genomic sequence. It does not detect balanced structural rearrangements such as translocations. It is based on isolation of genomic DNA isolated from test and reference cell populations, differential labeling with fluorescent dyes and cohybridization with a high-resolution DNA microarrays. Oligonucleotide CGH arrays enable genome-wide detection of DNA copy-number changes down to 15∼20 Kb.
Recurrent chromosomal rearrangements in various tumors define Minimal Critical Regions (MCR) that are often small enough to pinpoint the few candidate genes in oncogenesis that lie in this chromosomal region. Many of these MCR contain known oncogenes and tumor suppressor genes and also help identify new genes that are critical in lymphomagenesis.
In this study, whole-genome 44K and 244K oligonucleotide arrays were used for fine mapping of recurrent copy number alterations (CNA) present in a set of 27 human Burkitt's lymphomas. These results allow a global view of genomic instability at a high level resolution (50 Kb on average) and the identification of new gene loci that are recurrently altered.
Materials and Methods
Primary Tumors and Cell Lines
The patients were studied according to various protocols approved by the French Ethics Committees. Between 1995 and 2005, 13 BL tumors were collected and frozen at disease onset. Tumor specimens were reviewed by an expert hematopathologist according to current WHO criteria for morphologic, immunophenotypic, and cytogenetic features.
Fifteen Burkitt's lymphoma-derived B cell lines were studied: BL2, BL31, BL41, BL70, BL84, BL104, BLLAL, Ly47, Ly91, Namalwa, Ramos, Oku, Salina, Seraphina and BLMer (derived from the relapse of tumor case 29124, thus counting one BL for both samples). They were cultured at 37°C in complete medium (GIBCO-BRL, Scotland) containing 2 mM L-glutamine, 1 mM pyruvate, 20 mM glucose, 20 µg/ml gentamicine and supplemented with 5% heat-inactivated fetal calf serum.
Cytogenetic analysis was performed on metaphase spreads obtained from tumor biopsy or bone marrow specimens and cell lines. In each case, 15 RHG-banded metaphases were analyzed when possible. Clonal chromosomal abnormalities were described according to the International System for Human Cytogenetic Nomenclature .
Fluorescence in situ hybridization (FISH) studies
A set of commercial probes was used to diagnosis in some cases and to resolve discrepancies between aCGH results and karyotype data (Supplementary Table S1). To confirm DNA copy number changes obtained from aCGH, a FISH analysis was carried out using individual probes on cytogenetic preparations. Five BAC clones (Table S1) were selected from Pieter De Jong libraries (http://www.chori.org/bacpac/) according to their position on the UCSC human genome (http://www.genome.ucsc.edu/). Purified BAC DNA was labeled by random priming in the presence of Alexa 488-dUTP (green) and Alexa 594-dUTP (red) (Abbott). The quality of the probes was verified by hybridization to metaphase spreads of a negative control. The preparations were observed with an epifluorescence microscope and images captured with a Vysis imaging station. Between 20 and 40 metaphase spreads and 100 nuclei were analyzed for each sample.
Tumor genomic DNA was isolated according to Qiagen protocols with modifications . Samples containing a t(8;V) were detected by cytogenetic analysis and/or FISH (dissociation of the MYC signal) in more than 60% of cells. High-molecular-weight genomic DNA was extracted from the cell lines with a DNeasy extraction kit (Qiagen).
Patient tumor samples and cell lines were analyzed using 44K and 244K microarrays (Agilent Technologies, Santa Clara, CA, USA) respectively. Only cell lines were processed as dye-swap pairs. In all experiments, sex-matched DNA from a pooled human female or male individual (Promega, Madison, WI) was used as the reference. Oligonucleotide aCGH processing was performed as detailed in the manufacturer's protocol (version 4.0; http://www.agilent.com). Data were extracted from scanned images using feature extraction software (version A.8.5.3, Agilent). Raw data text files from the latter were then imported for analysis into CGH Analytics 3.4.40. Aberrations were detected with the ADM2 algorithm and filtering options of a minimum of 5 probes and abs(log2Ratio) >0.3. Aberration segments were individually reviewed using build 35, hg17 of UCSC . Anomalies that were localized to regions with high-copy repetitive or GC-rich DNA sequences including telomeric regions were excluded. We defined gains and losses for the oligonucleotide dataset as a linear ratio ≥1.2 or ≤0.8 respectively. High and low-level amplification events were defined as a linear ratio ≥4 or 2< ratio <4 respectively. The data are described in accordance with MIAME guidelines and have been deposited in ArrayExpress under E-TABM-703 accession number.
The karyotype of the primary tumor was complex in 4 cases (Table 1).
Among the 15 cell lines, 13 exhibited a complex karyotype (Table 2); 11 harbored the same chromosomal abnormalities that were previously described , , while BLLAL, BLMER, OKU and Salina have not been reported elsewhere. The hallmark t(8;14) translocation was detected in 18 tumors or cell lines. The variant t(8;22) translocations were found in 1 tumor and 5 cell lines and the t(2;8) were found in 2 tumors and 1 cell line.
aCGH CNA correlate with conventional and molecular cytogenetic results.
A strong correlation between aCGH results and karyotype analysis was observed for CNA ≥5 Mb. Some larger CNA were detected in cell lines exclusively by aCGH. For example, in the Ly47 cell line, a gain of a 25.75 Mb region on 16q21qter <62.92–88.67> could not be detected by karyotype analysis (Fig. 1 A+B). This gain was confirmed by FISH (Fig. 1C). In the Ly91 cell line, a nonsegmented gain of 1q was observed on aCGH (Fig. 1E) that allowed FISH to identify a der(13)t(1;13)(q32.1;p12) present in only 20% of the cells (Fig. 1 D+F). Often aCGH refined the breakpoints detected by conventional cytogenetics. In addition to primary BL translocations, four other balanced translocations were only shown by karyotype analysis without any visible scar at 20 Kb resolution (Table 2).
A RHG-banded karyotype of Ly47 showing 2 normal chromosome 16 (arrow). B Karyogramme of Ly47 cell line showing a gain of chromosome 16 (arrow). C FISH with whole painting of chromosome 16 confirmed aCGH result. The additional piece of chromosome 16 was translocated at the long arm of chromosome 11. D RHG-banded karyotype of Ly91 showing an add(13)(p12) which is presented in 20% of cells (arrow). E Karyogramme of Ly91 cell line showing a non segmented gain on 1q (arrow). F FISH with whole painting of chromosome 1(red) and 13(green) allowed to identify the origin of the material translocated at 13p. Hence, the add(13) became a der (13)t(1;13)(q32.1;p12).
Analysis of small-scale copy number alteration: CNVs
Small sized CNA (n = 145) defined as ≤2 Mb long, ranging from 0.03 to 2 Mb in size, were detected in 140 occurrences originating from 244 K arrays, and 5 from 44 K. More than half of these small CNA were found to be Mendelian CNVs. This was tested by examining the sequence present at each CNA, with the University of California at Santa Cruz Genome Browser (http://genome.cse.ucsc.edu) and Toronto (http://projects.tcag.ca/variation) tools. Eighty one of the 145 small CNA were mapped to Mendelian CNV (mCNV) in the Database of Genomic Variants. Among these mCNV, there were very frequent CNVs overlapping the large group of olfactory receptor genes, the UGT2B17 and UGT2B28 genes. The genes encoding the HLA group were also detected within these common CNVs. In our set, the GSTT1 gene exhibited the most frequent CNVs. This locus was gained (5 copies) in 7 cases and homozygously lost in three cases.
Immunoglobulin Somatic Copy Number Variation
Thirty-eight of the small CNA were mapped to the immunoglobulin loci at chromosomal subbands 2p11.2 (IGK), 14q32.3 (IGH) and 22q11.2 (IGL) (Table S2, Figure 2, Figure S1, Figure S2, Figure S3, Figure S4, Figure S5, Figure S6, Figure S7, Figure S8, Figure S9, Figure S10, Figure S11). These acquired monoclonal alterations were ascertained only by the 244 K aCGH platform. The IGH locus was rearranged in all cell lines (14/15 on the two chromosomes), with a specific pattern for each cell line and it expressed surface IgM. In addition, IGK gene rearrangements were also detected in all cell lines (8/15 biallelic). IGK was homozygously rearranged in the only t(2;8). IGL locus somatic rearrangements were observed in 10 cell lines (7 biallelic). The other 5 cell lines, whose IGL locus was in germline configuration, only exhibited surface IgK expression (Table S2). In the t(8;22) the IGL gene was rearranged on the two alleles in 4 cases. The only hemizygote was observed in the OKU cell line (Figure S8) which expressed IGK. These results, consistent with the germinal center nature of BL cells, and the processes of maturation of immunoglobulin, show a very good concordance between immunophenotype and aCGH results.
The immunoglobulin loci at chromosomal subbands 2p11.2 (IGK), 14q32.3 (IGH) and 22q11.2 (IGL) showed acquired monoclonal alterations by the 244 K aCGH platform. The segmented rearrangements were identified by a stained rectangle and the individual oligonucleotides are identified by a bold dot. The Y axis is the chromosomal position, the X axis is the ratio (loss is to the left). A to D, S1: The IGH locus was rearranged in all cell lines (14/15) on the two chromosomes, with a specific pattern for each cell line. BL2 and BL31 have a t(8;14). A to D, S1: IGK gene rearrangements were detected in all cell lines and 8 have a clear biallelic rearrangement including LY91 the only t(2;8). A to D, S1: IGL loci somatic rearrangements were observed in only 10 cell lines (7 biallelics) including four cases of t(8;22) as in BLAL. Other cell lines are presented as supporting figures.
Other Small Somatic Copy Number Alterations
Among the 26 remaining small-scale CNA (Tables 1 and 2), 16 imbalances were localized to either intergenic regions or predicted gene sequences of unknown function. The significance of these CNA remains to be determined. Ten imbalances were mapped to regions containing characterized genes, many of which play roles in tumorigenesis and B-cell differentiation and proliferation. These genes allowed either the narrowing of the minimal critical regions or remained as a single CNA (see below).
Analysis of large-scale copy number alteration
The total number of CNA >2 Mb was 136 in cell lines and tumors (Table 1). There were 17 whole chromosome gains or losses and 119 structural CNA (sCNA). The distribution of these sCNA was bimodal, with primary tumors or cell lines tending to have either a large number or no large CNA. From the frequency analysis, we observed the general trends of 1q (12/27), 13q (7/27), 7q (6/27), 8q (4/27), 2p (3/27), 11q (2/27) and 15q (2/27) gains whereas losses were more frequent at the following chromosomal regions: 3p (5/27), 4p (4/27), 4q (4/27), 9p (4/27), 13q(4/27), 6p (3/27), 17p (3/27), 6q (2/27), 11pterp13 (2/27) and 14q12q21.3 (2/27).
Minimal critical region altered in BL
Some of these CNA were recurrent across different samples and allowed us to define 21 MCRs of gain/amplification or loss/deletion (Table 3). A nearly equal number of lost and gained MCR were defined and their median size was 4.96 Mb (range 0.04–71.36 Mb).
The most frequent gained region was 1q followed by 13q. Three different MCRs were mapped to 1q. The proximal one was mapped to 1q21.1q25.2. The BL 41 cell line exhibited a 6.3 Mb amplicon localized at 1q21.1q21.3 <142.87–149.17 Mb> (Fig. 3A+B+C). The region exhibiting the highest copy number (as many as 7 copies), contained 14 genes and among them, BCA2 and PIAS3 seemed to be possible oncogenes. Two other MCRs were isolated on 1q (Table 3). Three different MCRs were mapped to 13q (Table 3). The most frequent one mapped to 13q31.3q32.1, contained the Namalwa cell line amplicon (Fig. 3F). A 240 Kb MCR at 8q24.2 encompassed MYC. A loss of 13q33.1q34 was only found in tumors. In our set, the most frequent deleted region was 3p14.2 followed by 9p21.3 and a deletion of 17p (Table 3).
A. Copy number profile of 1q21.1 amplicon in BL41:the 7 copies maximal amplitude region was the <142,87–143,28 Mb> interval that included BCA2 and PIAS3 genes. B and C. The amplifications of BCA2 and PIAS3 were confirmed by FISH with BAC clones RP11-767O2 and RP11-74F4 respectively which showed a small HSR. D and E. In Namalwa a low level amplification (4 to 5 copies) of 1q25.2 <176,6–177> was confirmed by FISH with BAC clone RP11-175C8 which contains the LHX4 gene only. 3F. In Namalwa the 13q31.33q2.1 <89,58–95,82> region was amplified. The proximal part that harboured the polycistron mir-17 and a part of GPC5 gene was present in 8 copies. The distal region, with maximal amplitude (12 copies), contained 12 genes including GPC5, GPC6 and ABCC4 (see text). G and H. In BL 41 the REL gene was found amplified and FISH with BAC clone RP11-373L24 showed a small HSR. I and J Molecular cytogenetic on BLMer cell line showing a duplication of BIRC6. RP11-121M15 BAC clone was choosen on the region of BIRC6. The latter gene was duplicated as denoted by an arrow.
In this work, we report a fine mapping of chromosomal imbalances in a set of 27 BL primary tumors and cell lines. Whole-genome 44 K and 244 K oligonucleotide arrays were used to analyze recurrent copy number alterations present in tumors and cell lines respectively.
Comparison of the 15 dye-swap pairs from cell lines revealed identical aberrations. In most cases, the boundaries of the chromosomal aberrations were absolutely identical. The dye-swap helped to reduce the background level. Whole-genome oligonucleotide microarrays aCGH analysis allowed us to delineate the chromosomal imbalances at 15∼20 Kb resolution in the 15 cell lines (244 K) and at 70 Kb average resolution in the 13 primary tumors (44 K).
Each sample was also investigated using conventional cytogenetics. Close agreement between karyotype data and whole-genome aCGH CNAs was observed. When discrepancies occurred, they were mostly explained, i) by a lack of detection due to karyotype resolution; ii) by aCGH locally blurred heterogeneous cell clones, or normal cell contamination in the sample; iii) by the presence of balanced chromosomal rearrangements. In addition to BL hallmark anomalies, 4 other apparently balanced translocations were detected exclusively by conventional cytogenetics, as expected (Table 2). The karyotypes of the 11 previously published cell lines were essentially identical to those reported elsewhere , .
Four cell lines (BLLAL, OKU, SALINA, BLMer) have been karyotyped for the first time in the present paper. The BLMer cell line was established from a recurrent tumor (case 29124). Karyotype analysis of BLMer showed the same anomalies as observed in the parental tumor (data not shown). Although cell lines cannot fully recapitulate all the biological aspects of tumors , the chromosomal alterations observed in cell lines are representative of their parent histology .
In the primary tumor subgroup with CNA ≥1 (n = 6), the mean number of CNA per sample was 6 versus 9 for cell lines (n = 14), showing the same order of magnitude.
Partial duplication of the PVT1 mi RNA locus: a role in BL?
In the BL 84 and Ly 47 cell lines, the der(8)t(8;22) was totally and partially duplicated respectively. This allowed the high-resolution mapping of the translocation. The MYC 3′ breakpoints were mapped to the PVT1 locus that harbors mir1204, mir1205, mir1206 and mir1207 . Among these genes, only mir1204 and mir1205 were duplicated in the two cell lines (Fig. 4). The localization of these micro-RNAs in the 8q24 region raises questions. Are these micro-RNA implicated in lymphomagenesis. Are BL variant translocations a distinct group?
MYC region breakpoints. In Seraphina, BL 84 and Ly 47 cell lines, MYC locus was gained. MYC region breakpoints vary with primary anomalies. In BL 84 and Ly 47 cell lines with t(8;22), MYC breakpoints were mapped to PVT1(Huppi et al., 2008); mir-1205 and mir1204 (not present on UCSC map, located on chr8:128877390-128877456) defined the MCR of this gain. In Seraphina, a 240 Kb duplication was observed around MYCand located by FISH in the immediate vicinity this gene on the der(14)t(8;14) (data not shown). This duplication contained hsa mir 1204.
CNAs and CNVs in BL
A total of 145 CNA ≤2 Mb were detected. Among them, there were 81 CNA mapped to Mendelian CNV. GSTT1 was the most polymorphic gene in our set. GSTT1 is a member of a multigenic family of detoxifying enzymes . Four classes of isoenzymes have been linked to human malignancy: GSTM, GSTP, GSTA and GSTT. Several studies have shown that individuals who harbor GSTM1−/− or GSTT1−/− as well as GSTP1 variants are at an increased risk for a series of tumors , . Further studies are required to clarify its role in Burkitt's lymphoma.
CNV are not totally neutral variants. Many recent studies have directly and indirectly implicated some Mendelian CNV in genomic disorders , . A 560 Kb low-level amplification of the 2p23 region was found in the BL Mer cell line and its parental tumor, and confirmed by FISH (Figure 3 I, J). This region harbors only BIRC6 gene which encodes an inhibitor of apoptosis and a chimeric E2/E3 ubiquitin ligase . However, the 3 copies on chromosome 2 and the single copy on the other chromosome have the same boundaries as a reported CNV. Interestingly, germline amplification of BIRC6 was found in patients with a family history of pancreatic cancer .
Somatic CNVs mapped to the immunoglobulin locus ,  were the second type found in our study. BLs have clonally rearranged IGH, IGK and IGL chain genes, hence these rearrangements can be detected by the 244 K aCGH platform that showed a high sensitivity between the oligonucleotide arrays . The rearranged pattern is highly specific of each individual malignant cell line confirming that they are monoclonal proliferations. Several oligonucleotides have an intermediate value between one and two copy losses (Figure 2, Figure S1, Figure S2, Figure S3, Figure S4, Figure S5, Figure S6, Figure S9). This could be related to further rearrangements of IG genes in subclones. According to the genetic model of lymphomagenesis, the frozen differentiation stage might reflect the cell type in which the primary translocation t(8;14) took place leading to maturation arrest. An alternative hypothesis is that mature aggressive B-NHL originate from cells with stem-cell features or that stemness was acquired during lymphomagenesis by epigenetic remodeling .
In our study, the distribution of the CNA >2 Mb was bimodal, with 6/12 primary tumors and 13/15 cell lines exhibiting additional abnormalities to the IG/MYC translocation, in accordance with the low number of chromosomal changes previously reported , . A smaller group with partial uniparental disomy resulting in loss of heterozygosity without chromosomal imbalances besides classic chromosomal instability, has been reported in BL .
Gains were more frequent at the following regions 1q (12/27), 13q (7/27), 7q (6/27), 8q (4/27), 2p (3/27), 11q (2/27) and 15q (2/27). Ten MCRs were observed.
MCRs on chromosome 1
Three MCRs were observed on 1q. The major one was localized at 1q21.1q25.2 <142.87–177.00>. In a recent study using tiling-resolution aCGH, this MCR was larger and mapped to 1q12q25.2 . In the BL 41 cell line, the 1q21.1q21.3 <142.87–149.17> region harbored a 6 Mb amplicon (Fig. 3A, B, C). In a recent study, a 1.35 Mb minimal deleted region <143.65–145.00>, was associated with developmental defects . This region overlapped the BL41 amplicon, but the copy number peaks (<142.87–143.28>) seemed to be different. The genomic structure of 1q21 is extremely complex, with at least 4 large segmental-duplication blocks ranging in size from 270 Kb to 2.2 Mb. Consequently, these duplicons favored the non-allelic homologous recombination which might explain both the congenital anomalies (Mefford et al., 2008) and malignancy, particularly lymphoma. Although 1q rearrangements have no impact on the prognosis of BL , amplification does nevertheless contribute to tumor development . BCA2 and PIAS3 that mapped at the maximal amplitude of the BL 41 amplicon are thus candidate driver genes in this amplicon. BCA2 has E3 ubiquitin ligase activity and was found to be overexpressed in invasive breast cancer . PIAS3 codes for the Protein Inhibitor of Activated STAT3. PIAS3 signaling has been shown to prevent apoptosis and enhance cellular proliferation through the regulation of genes such as MYC . Overactivation of STAT3 has been identified in many cancers .
The second 1q MCR was 1q32.1 <200.97–201.36>. It encompasses MDM4. This gene has not been implicated in BL, but MDM4 inhibits P53, and in vivo development of B-cell lymphomas in Eμ-myc MDM4+/− mice is delayed compared to that occurring in Eμ-myc MDM4+/+ mice .
The third 1q MCR was 1q44 <240.24–241.35>. It harbors AKT3 which is one of the 3 isoforms of AKT. The latter is a Ser/Thr kinase in the PTEN/PI3K/AKT pathway and activation of AKT is often observed in human cancers . In primary hepatocellular carcinoma, AKT3 was up-regulated as a result of a gained 1q44 region . In the Namalwa cell line, a 0.4 Mb low-level amplification (5 copies) at 1q25.2 <176.6–177> containing 5 genes was detected. Among them, LHX4, a LIM homeobox 4 was found to be amplified by FISH (Fig. 3D+E). LHX4 is the partner of IGH in t(1;14)(q25;q32) detected in pre-B acute lymphoblastic leukemia .
MCRs on other chromosomes
The second most gained arm was 13q. The major MCR was 13q31.3q32.1 <89.58–96.81> containing a 6.24 Mb amplicon <89.58–95.82> (Fig. 3F) as frequently seen in lymphomas but also across a broad range of tumor types . The proximal segment of this amplicon contained microRNA-17 polycistron ,  which was stably upregulated in the presence of constitutive MYC expression . The highest copy number segment harbored 12 genes, including GPC5, GPC6 and ABCC4. Glypican genes (GPC5 and GPC6), belong to a family of heparan sulfate proteoglycans that are constantly expressed and up-regulated in rhabdomyosarcoma with an amplified 13q31q32 region . However, in case 29147 and in Namalwa cell line, the DNA copy number of GPC5 argues against this gene as the driver of the amplification. ABBC4 (MRP4), which encodes the multi-drug resistance protein, could also play this role. It was present in 12 copies in the Namalwa cell line. It was found to be amplified in several drug-resistant cell lines derived from various malignancies . ABBC4 transcripts were found upregulated in a set of BL with gain of 13q31q32 region .
On chromosome 8, MYC was the only gene contained in a 240 Kb gained MCR at 8q24.2, originating either from t(8;14) and t(8;22) rearrangements.
A 2p16 MCR contained a 40 Kb amplicon in BL 41. It harbored REL (Fig. 3G, H), a member of the NF-κB family of transcription factors, which was found to be amplified in primary mediastinal B-cell lymphoma .
The 11q23.1 MCR mapped to <110.62–110.81> contained POU2AF1, a B-cell specific transcriptional coactivator which is amplified in multiple myeloma .
A 1.8 Mb amplicon was found at 17p11.2, in the BL 41 cell line, with a 381 Kb peak of 10 copies <20.72–21.10> containing four genes of mostly unknown function. The proximal and distal boundaries of this amplicon with 7 copies, contained two kinases genes, AKAP10 and MAP2K3. The genomic structure of 17p11 is complex, with several segmental-duplication blocks including one totaling 183 Kb.
Gene loss regions in BL
The most frequent deleted arm was 3p in four cell lines and one tumor, a rare finding in BL. A 100 Kb MCR localized at 3p14.2 <60.43–60.53> contained the 5th exon of FHIT (Fragile Histidine Triad) (Fig. 5), a finding in agreement with earlier studies .
Based on the UCSC data base (June, 2004), the 402 Kb MCR on 3p remove the fifth exon of FHIT gene.
On 9p, the 420 Kb MCR harbored CDKN2A/CDKN2B, a gene rarely reported to be impaired in BL .
On 13q33.1q34 a 10 Mb MCR, detected only in three tumors, harbored more than thirty genes. Among these ING1, COL4A2, COL4A1, RAB20, LIG4, TNFSF13B, all in a small region, could constitute the target of this loss. In case 29125 a single10 Mb deletion at 13q14.1q14.3 removed the critical mir-16-1 and mir-15a involved in CLL . This rearrangement is not observed in the 15 cell lines.
On 17p, a large 19.86 Mb MCR had removed P53 as well as several other genes. Mutations in the P53 gene have been found in at least 33% of BL biopsy specimens  and in as much as 83% of BL cell lines , .
Some single deletions were observed. In case 28787, a deletion at 2p23.1p16.3 particularly removed MSH2 and MSH6 genes that do not seem to be frequently altered in lymphoma . EBF1 (5q33.3 <158.08–158.18>), the early B cell factor was lost in Seraphina. In a recent work on ALL, mono-allelic deletions of EBF1 were emphasized .
High resolution aCGH is a powerful method that allowed a fine mapping of additional unbalanced chromosomal abnormalities in BL, but karyotype still remain an essential tool to rapidly identify balanced chromosomal translocations. A subgroup of BL without CNAs, warrants further investigation (SNP array, whole genome sequence) in order to find the necessary additional oncogenic events to the MYC rearrangement . The identification of the target genes of the large MCR will need correlations with other genomics data sets (gene expression, nucleotide sequence, epigenetic…) in order to make the low throughput functional gene studies.
With regard to additional chromosomal abnormalities studied by cytogenomics, BL appears to exhibit non-random genetic heterogeneity as revealed by this study. The MCRs remain to be fully functionally characterized in order to design targeted and personalized therapies in poor prognosis disease .
The microarray data analyzed in this paper have been submitted to the Array Express data repository at the European Bioinformatics Institute (http://www.ebi.ac.uk/arrayexpress/) under the following accession numbers: E-TABM-703.
Commercial and BAC probes used to validate aCGH results
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Clonal Immunoglobulin phenotype linked to chromosomal abnormalities and acquired CNV
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We are grateful to the help of Dr Alexender Valent, those of Bastien Job for some analysis, and to the gift of cell lines by Dr Alan B. Rickinson. The authors thank Lorna Saint Ange for editing and Cécile Tétaud, Bernard Clausse, Philippe Léopoldi, Didier Fauvet, Sébastien Forget, Yann Lécluse for their help.
Conceived and designed the experiments: VL AB. Performed the experiments: ST CR TR LDC JW. Analyzed the data: ST PD NI YV CP VR CP AB. Contributed reagents/materials/analysis tools: GD JB VR CP JW AB. Wrote the paper: ST PD JW AB.
- 1. Harris NL, Horning SJ (2006) Burkitt's lymphoma–the message from microarrays. N Engl J Med 354: 2495–2498.
- 2. Thorley-Lawson D, Allday M (2008) The curious case of the tumour virus: 50 years of Burkitt's lymphoma. Nat Rev Microbiol 6: 913–924.
- 3. Lenoir G, Preud'homme J, Bernheim A, Berger R (1982) Correlation between immunoglobulin light chain expression and variant translocation in Burkitt's lymphoma. Nature 298: 474–476.
- 4. Hummel M, Bentink S, Berger H, Klapper W, Wessendorf S, et al. (2006) A biologic definition of Burkitt's lymphoma from transcriptional and genomic profiling. N Engl J Med 354: 2419–2430.
- 5. Battey J, Moulding C, Taub R, Murphy W, Stewart T, et al. (1983) The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma. Cell 34: 779–787.
- 6. Aoufouchi S, Faili A, Zober C, D'Orlando O, Weller S, et al. (2008) Proteasomal degradation restricts the nuclear lifespan of AID. J Exp Med 205: 1357–1368.
- 7. Lones MA, Sanger WG, Le Beau MM, Heerema NA, Sposto R, et al. (2004) Chromosome abnormalities may correlate with prognosis in Burkitt/Burkitt-like lymphomas of children and adolescents: a report from Children's Cancer Group Study CCG-E08. J Pediatr Hematol Oncol 26: 169–178.
- 8. Heerema NA, Bernheim A, Lim MS, Look AT, Pasqualucci L, et al. (2005) State of the Art and Future Needs in Cytogenetic/Molecular Genetics/Arrays in childhood lymphoma: summary report of workshop at the First International Symposium on childhood and adolescent non-Hodgkin lymphoma, April 9, 2003, New York City, NY. Pediatr Blood Cancer 45: 616–622.
- 9. Poirel HA, Cairo MS, Heerema NA, Swansbury J, Auperin A, et al. (2009) Specific cytogenetic abnormalities are associated with a significantly inferior outcome in children and adolescents with mature B-cell non-Hodgkin's lymphoma: results of the FAB/LMB 96 international study. Leukemia 23: 323–331.
- 10. Kallioniemi A (2008) CGH microarrays and cancer. Curr Opin Biotechnol 19: 36–40.
- 11. Chin L, Gray JW (2008) Translating insights from the cancer genome into clinical practice. Nature 452: 553–563.
- 12. Shaffer L, Tommerup N (2005) An International System For Human Cytogenetic Nomenclature. Basel: Karger.
- 13. Bernheim A, Toujani S, Saulnier P, Robert T, Casiraghi O, et al. (2008) High-resolution array comparative genomic hybridization analysis of human bronchial and salivary adenoid cystic carcinoma. Lab Invest 88: 464–473.
- 14. Karolchik D, Hinrichs A, Furey T, Roskin K, Sugnet C, et al. (2004) The UCSC Table Browser data retrieval tool. Nucleic Acids Res 32: 493–496.
- 15. Berger R, Bernheim A (1985) Cytogenetics of Burkitt's lymphoma-leukaemia: a review. IARC Sci Publ 65–80.
- 16. Karpova MB, Schoumans J, Blennow E, Ernberg I, Henter JI, et al. (2006) Combined spectral karyotyping, comparative genomic hybridization, and in vitro apoptyping of a panel of Burkitt's lymphoma-derived B cell lines reveals an unexpected complexity of chromosomal aberrations and a recurrence of specific abnormalities in chemoresistant cell lines. Int J Oncol 28: 605–617.
- 17. Greshock J, Nathanson K, Martin AM, Zhang L, Coukos G, et al. (2007) Cancer cell lines as genetic models of their parent histology: analyses based on array comparative genomic hybridization. Cancer Res 67: 3594–3600.
- 18. Huppi K, Volfovsky N, Runfola T, Jones TL, Mackiewicz M, et al. (2008) The identification of microRNAs in a genomically unstable region of human chromosome 8q24. Mol Cancer Res 6: 212–221.
- 19. Clapper ML (2000) Genetic polymorphism and cancer risk. Curr Oncol Rep 2: 251–256.
- 20. Morari EC, Leite JL, Granja F, da Assumpcao LV, Ward LS (2002) The null genotype of glutathione s-transferase M1 and T1 locus increases the risk for thyroid cancer. Cancer Epidemiol Biomarkers Prev 11: 1485–1488.
- 21. Sebat J (2007) Major changes in our DNA lead to major changes in our thinking. Nat Genet 39: S3–5.
- 22. Stranger BE, Forrest MS, Dunning M, Ingle CE, Beazley C, et al. (2007) Relative impact of nucleotide and copy number variation on gene expression phenotypes. Science 315: 848–853.
- 23. Ren J, Shi M, Liu R, Yang QH, Johnson T, et al. (2005) The Birc6 (Bruce) gene regulates p53 and the mitochondrial pathway of apoptosis and is essential for mouse embryonic development. Proc Natl Acad Sci U S A 102: 565–570.
- 24. Lucito R, Suresh S, Walter K, Pandey A, Lakshmi B, et al. (2007) Copy-number variants in patients with a strong family history of pancreatic cancer. Cancer Biol Ther 6: 1592–1599.
- 25. Bernheim A, Dessen P, Lazar V, Auger N, Fauvet D, et al. (2007) Cryptic del(13q14.2) and physiological deletions of immunoglobulin genes detected by high-resolution array comparative genomic hybridization in a patient with indolent chronic lymphocytic leukemia. Cancer Genet Cytogenet 176: 89–91.
- 26. Wang K, Li M, Hadley D, Liu R, Glessner J, et al. (2007) PennCNV: an integrated hidden Markov model designed for high-resolution copy number variation detection in whole-genome SNP genotyping data. Genome Res 17: 1665–1674.
- 27. Coe BP, Ylstra B, Carvalho B, Meijer GA, Macaulay C, et al. (2007) Resolving the resolution of array CGH. Genomics 89: 647–653.
- 28. Martin-Subero JI, Kreuz M, Bibikova M, Bentink S, Ammerpohl O, et al. (2008) New insights into the biology and origin of mature aggressive B-cell lymphomas by combined epigenomic, genomic and transcriptional profiling. Blood.
- 29. Nielaender I, Martin-Subero JI, Wagner F, Martinez-Climent JA, Siebert R (2006) Partial uniparental disomy: a recurrent genetic mechanism alternative to chromosomal deletion in malignant lymphoma. Leukemia 20: 904–905.
- 30. Salaverria I, Zettl A, Bea S, Hartmann EM, Dave SS, et al. (2008) Chromosomal alterations detected by comparative genomic hybridization in subgroups of gene expression-defined Burkitt's lymphoma. Haematologica 93: 1327–1334.
- 31. Davidsson J, Andersson A, Paulsson K, Heidenblad M, Isaksson M, et al. (2007) Tiling resolution array comparative genomic hybridization, expression and methylation analyses of dup(1q) in Burkitt lymphomas and pediatric high hyperdiploid acute lymphoblastic leukemias reveal clustered near-centromeric breakpoints and overexpression of genes in 1q22-32.3. Hum Mol Genet 16: 2215–2225.
- 32. Mefford HC, Sharp AJ, Baker C, Itsara A, Jiang Z, et al. (2008) Recurrent rearrangements of chromosome 1q21.1 and variable pediatric phenotypes. N Engl J Med 359: 1685–1699.
- 33. Albertson DG (2006) Gene amplification in cancer. Trends Genet 22: 447–455.
- 34. Burger AM, Gao Y, Amemiya Y, Kahn HJ, Kitching R, et al. (2005) A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer. Cancer Res 65: 10401–10412.
- 35. Bowman T, Broome MA, Sinibaldi D, Wharton W, Pledger WJ, et al. (2001) Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis. Proc Natl Acad Sci U S A 98: 7319–7324.
- 36. Inghirami G, Chiarle R, Simmons WJ, Piva R, Schlessinger K, et al. (2005) New and old functions of STAT3: a pivotal target for individualized treatment of cancer. Cell Cycle 4: 1131–1133.
- 37. Terzian T, Wang Y, Van Pelt CS, Box NF, Travis EL, et al. (2007) Haploinsufficiency of Mdm2 and Mdm4 in tumorigenesis and development. Mol Cell Biol 27: 5479–5485.
- 38. Koseoglu S, Lu Z, Kumar C, Kirschmeier P, Zou J (2007) AKT1, AKT2 and AKT3-dependent cell survival is cell line-specific and knockdown of all three isoforms selectively induces apoptosis in 20 human tumor cell lines. Cancer Biol Ther 6: 755–762.
- 39. Kim TM, Yim SH, Shin SH, Xu HD, Jung YC, et al. (2008) Clinical implication of recurrent copy number alterations in hepatocellular carcinoma and putative oncogenes in recurrent gains on 1q. Int J Cancer 123: 2808–2815.
- 40. Kawamata N, Sakajiri S, Sugimoto KJ, Isobe Y, Kobayashi H, et al. (2002) A novel chromosomal translocation t(1;14)(q25;q32) in pre-B acute lymphoblastic leukemia involves the LIM homeodomain protein gene, Lhx4. Oncogene 21: 4983–4991.
- 41. Neat MJ, Foot N, Jenner M, Goff L, Ashcroft K, et al. (2001) Localisation of a novel region of recurrent amplification in follicular lymphoma to an approximately 6.8 Mb region of 13q32-33. Genes Chromosomes Cancer 32: 236–243.
- 42. Croce CM, Calin GA (2005) miRNAs, cancer, and stem cell division. Cell 122: 6–7.
- 43. He L, Thomson JM, Hemann MT, Hernando-Monge E, Mu D, et al. (2005) A microRNA polycistron as a potential human oncogene. Nature 435: 828–833.
- 44. Tagawa H, Karube K, Tsuzuki S, Ohshima K, Seto M (2007) Synergistic action of the microRNA-17 polycistron and Myc in aggressive cancer development. Cancer Sci 98: 1482–1490.
- 45. Williamson D, Selfe J, Gordon T, Lu YJ, Pritchard-Jones K, et al. (2007) Role for amplification and expression of glypican-5 in rhabdomyosarcoma. Cancer Res 67: 57–65.
- 46. Yasui K, Mihara S, Zhao C, Okamoto H, Saito-Ohara F, et al. (2004) Alteration in copy numbers of genes as a mechanism for acquired drug resistance. Cancer Res 64: 1403–1410.
- 47. Weniger MA, Gesk S, Ehrlich S, Martin-Subero JI, Dyer MJ, et al. (2007) Gains of REL in primary mediastinal B-cell lymphoma coincide with nuclear accumulation of REL protein. Genes Chromosomes Cancer 46: 406–415.
- 48. Zhao C, Inoue J, Imoto I, Otsuki T, Iida S, et al. (2008) POU2AF1, an amplification target at 11q23, promotes growth of multiple myeloma cells by directly regulating expression of a B-cell maturation factor, TNFRSF17. Oncogene 27: 63–75.
- 49. Ohta M, Inoue H, Cotticelli MG, Kastury K, Baffa R, et al. (1996) The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 84: 587–597.
- 50. Tresnasari K, Takakuwa T, Ham MF, Rahadiani N, Nakajima H, et al. (2007) Telomere dysfunction and inactivation of the p16(INK4a)/Rb pathway in pyothorax-associated lymphoma. Cancer Sci 98: 978–984.
- 51. Gaidano G, Ballerini P, Gong JZ, Inghirami G, Neri A, et al. (1991) p53 mutations in human lymphoid malignancies: association with Burkitt lymphoma and chronic lymphocytic leukemia. Proc Natl Acad Sci U S A 88: 5413–5417.
- 52. Wiman KG, Magnusson KP, Ramqvist T, Klein G (1991) Mutant p53 detected in a majority of Burkitt lymphoma cell lines by monoclonal antibody PAb240. Oncogene 6: 1633–1639.
- 53. Sobol H, Benziane A, Kerangueven F, Yin L, Noguchi T, et al. (2002) Genome-wide search for loss of heterozygosity in Burkitt lymphoma cell lines. Genes Chromosomes Cancer 33: 217–224.
- 54. Brimmell M, Mendiola R, Mangion J, Packham G (1998) BAX frameshift mutations in cell lines derived from human haemopoietic malignancies are associated with resistance to apoptosis and microsatellite instability. Oncogene 16: 1803–1812.
- 55. Mullighan CG, Goorha S, Radtke I, Miller CB, Coustan-Smith E, et al. (2007) Genome-wide analysis of genetic alterations in acute lymphoblastic leukaemia. Nature 446: 758–764.