The cytoplasm of eukaryotic cells is a highly dynamic compartment where membranes readily undergo fission and fusion to reorganize the cytoplasmic architecture, and to import, export and transport various cargos within the cell. The double membrane of the nuclear envelope that surrounds the nucleus, segregates the chromosomes from cytoplasm and regulates nucleocytoplasmic transport through pores. Many details of its formation are still unclear. At fertilization the sperm devoid of nuclear envelope pores enters the egg. Although most of the sperm nuclear envelope disassembles, remnants of the envelope at the acrosomal and centriolar fossae do not and are subsequently incorporated into the newly forming male pronuclear envelope. Remnants are conserved from annelid to mammalian sperm.
Using lipid mass spectrometry and a new application of deuterium solid-state NMR spectroscopy we have characterized the lipid composition and membrane dynamics of the sperm nuclear envelope remnants in isolated sperm nuclei.
We report nuclear envelope remnants are relatively fluid membranes rich in sterols, devoid of sphingomyelin, and highly enriched in polyphosphoinositides and polyunsaturated phospholipids. The localization of the polybasic effector domain of MARCKS illustrates the non-nuclear aspect of the polyphosphoinositides. Based on their atypical biophysical characteristics and phospholipid composition, we suggest a possible role for nuclear envelope remnants in membrane fusion leading to nuclear envelope assembly.
Citation: Garnier-Lhomme M, Byrne RD, Hobday TMC, Gschmeissner S, Woscholski R, Poccia DL, et al. (2009) Nuclear Envelope Remnants: Fluid Membranes Enriched in STEROLS and Polyphosphoinositides. PLoS ONE 4(1): e4255. https://doi.org/10.1371/journal.pone.0004255
Editor: Richard Steinhardt, University of California, Berkeley, United States of America
Received: October 24, 2008; Accepted: December 23, 2008; Published: January 23, 2009
Copyright: © 2009 Garnier-Lhomme et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by Cancer Research UK core funding, an Amherst College Copeland Fellowship for RDB and an Amherst College Faculty Research Award from the H. Axel Schupf '57 Fund for Intellectual Life to DLP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
At fertilization, the sea urchin egg is activated by the sperm whose nucleus enters with an envelope devoid of pores. Most of this envelope is rapidly disassembled, its membranes vesiculating as the sperm chromatin decondenses from its compact conoïd shape to a uniformly euchromatic spherical mass. During this process, remnants of the sperm nuclear envelope at the tip and base of the nucleus, which line two cup-shaped cavities (the acrosomal and centriolar fossae), are not disassembled . Egg membranes bind to this nucleus and then fuse to form a nuclear envelope with pores, incorporating the polar sperm nuclear envelope remnants into the new male pronuclear envelope .
Similar remnant membranes are conserved from annelid to mammalian sperm , . Their function has mainly been studied in cell free preparations from fertilized sea urchin egg cytoplasm using a well-established system for nuclear envelope assembly on exogenously added sperm –. From these studies three major points have emerged: 1) without the envelope remnants the egg nuclear membrane precursor vesicles do not bind to the nucleus and thus nuclear envelope formation is prevented, 2) the binding of the egg nuclear membrane precursor vesicles proceeds progressively from the sites of the remnants in the acrosomal and centriolar fossae, eventually surrounding the entire surface , and 3) one subfraction of egg vesicles (MV1) is required for the remnants to be incorporated into the new nuclear envelope and can fuse with the remnants without the other vesicles . Thus the remnants are likely to have a role both in binding of precursor membranes and initiation of membrane fusion .
To mimic in vivo envelope disassembly, sperm nuclei were extracted with a 0.1% non-ionic detergent so that the lateral nuclear envelope was removed and the only membranous material left was at the two poles. We refer to these detergent treated sperm nuclei as 0.1% nuclei. It is important to note that the available evidence suggests these detergent-resistant membranous regions are not created by the extraction procedures since they are visible in untreated sperm, and both in vitro and in vivo studies have shown that the nuclear envelope remnants become incorporated into the newly formed nuclear envelope , , .
Initially the only physical property attributed to these regions was that they are by definition detergent-resistant membranes (DRMs). DRMs have been subject to a plethora of investigations and the current paradigm predicts that DRMs are typically enriched in cholesterol and saturated phospholipids . DRMs are commonly isolated from whole cell lysates by sucrose gradient centrifugation and purified using specific protein markers such as glycosyl-phosphoinositide (GPI)-anchored proteins –. DRMs obtained in this manner are enriched in cholesterol. Cholesterol confers resistance to detergent solubilization and increases phospholipid packing , .
Cholesterol has several functions in natural membranes, including acting as a scaffold for targeting various proteins , . Cholesterol is also a fusogenic lipid . Its fusogenic properties are due to its high spontaneous negative curvature and thus it has been suggested that when localized it can induce membrane fusion like other lipids displaying negative curvature such as diacylglycerol and phosphatidylethanolamine –.
Nuclear envelope remnants are essential for formation of the male pronucleus. During this process, a subset of membrane vesicles derived from the egg cortex called MV1, highly enriched in PLCγ and PtdIns(4,5)P2 , binds exclusively to the nuclear envelope remnant regions, and other membrane vesicles derived from the egg endoplasmic reticulum accumulate along the sides of the nucleus . All of these subsequently fuse to form a nuclear envelope with functional pores , .
In this paper we characterize the morphology, composition and dynamics of the remnants. We used lipid mass spectrometry to determine lipid composition and deuterium solid-state NMR spectroscopy to study membrane dynamics of the nuclear envelope remnants. We show they consist of two bilayers with an atypical composition of polyphosphoinositides: 12 mol% PtdInsP, 12 mol% PtdInsP2, and 9 mol% PtdInsP3 of total phospholipids. They are also rich in cholesterol (42 mol% of total lipids), lack sphingomyelin and are virtually devoid of fully saturated phospholipids. Their fluidity is distributed from liquid ordered to fluid states. We attribute their fluid characteristics primarily to the high fraction of cholesterol and phospholipids with polyunsaturated chains and low fraction of lipids with saturated chains. Moreover we show that by decreasing the average levels of cholesterol we affect nuclear envelope formation, indicating a role for high levels of cholesterol in membrane fusion.
Although our group has previously reported that MV1 also has atypically high amounts of phosphoinositides , we believe that this is the first time that natural membrane bilayers with both high levels of sterols and polyphosphoinositides and with fluid properties have been reported. Based on the unusual biophysical properties of the nuclear envelope remnants we suggest a possible role for these membranes in nuclear envelope formation.
Materials and Methods
Sea urchins Lytechinus pictus (L.pictus) and Strongylocentrotus purpuratus (S. purpuratus) were purchased from Marinus (Long Beach, CA). Paracentrotus lividus (P. lividus) were provided by the Unidade de Investigaçao de Biologia do Desenvolvimento (UIBD), Universidade Lusófona, Lisbon, Portugal. DiOC6 (3′3-dihexyloxacarbocyanine iodide) was from Invitrogen. The internal standards PtdIns(4)P (diC16, H+), PtdIns(4,5)P2 (diC16, H+), PtdIns(3,4,5)P3 (diC16, H+) were from Cell Signals, DiC16 PtdIns (monosodium salt) was from Echelon and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE), 1,2-dilauroyl-sn-glycero-3-[phospho-L-serine] (sodium salt) (DLPS), 1,2-dilauroyl-sn-glycero-3-phosphate (monosodium salt) (DLPA), 1,2-dilauroyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DLPG) were from Avanti Polar Lipids, Inc. The deuterium labelled POPC-2H31 was from Avanti Polar lipids, Inc. Methyl-beta cyclodextrin (MβCD) and filipin III were from Sigma-Aldrich. The Texas Red MARCKS peptide (residues 151-175) has been previously described , and was a gift from Stuart McLaughlin.
The 0.1% nuclei were prepared as previously described . Briefly, 250 μl of concentrated viable sperm was suspended in 10ml of ice-cold SXN buffer (50mM HEPES, 250mM sucrose, 150mM NaCl, 300mM glucose, 0.5mM spermidine, 0.15mM spermine, pH 7.2) and centrifuged at 2,600g for 5 min at 4°C. The pellet was resuspended in 1.5ml SXN and sonicated in an Ultrasonic bath for 6 min. Sperm were centrifuged at 2,600g, 4°C for 1.5 min. The pellets were resuspended in 990 μl SXN and 10 μl of 10% Triton X-100 and further mixed for 30 min at room temperature. These nuclei are referred to as 0.1% nuclei. The nuclei were centrifuged at 2,600g for 2 min at 4°C, washed twice with 1ml of SXN buffer at 2,600g, 1 min at 4°C and finally resuspended in 500 μl SXN plus 500 μl freezing buffer. This suspension was thoroughly homogenized, cryogenically frozen in liquid nitrogen and stored at −80°C.
The use of Triton X-100 in this procedure, and potential nuclei extract contamination does not appear to be detrimental to subsequent reactions based on the following observations: 1) 0.1% nuclei are repeatedly washed after Triton extraction (see above). 2) 0.1% and 1% Triton X-100 extracted nuclei both decondense at the same rate in a soluble fertilized egg cytosolic fraction (S150), indicating that nuclear architecture is intact after exposure to Triton . 3) NERs can be removed from 0.1% nuclei with 1% Triton X-100, and subsequently reassembled into the acrosomal and centriolar fossae, indicating that exposure to high Triton concentrations does not alter NER structure and function .
The 0.1% nuclei stored at −80°C were thawed on ice, centrifuged for 2 min at 1,500g, 4°C and gently resuspended in 50 μl Tris-saline buffer (TN: 10mM Tris-HCl, 150mM NaCl, pH 7.2) to a concentration of approximately 6×107 nuclei/ml. Decondensation assays typically consisted of adding 3 μl of nuclei to 20 μl of egg cytoplasm (S10) supplemented with 1.2 μl of ATP-generating system (ATP-GS: 33mM ATP, 333mM creatine phosphate and 0.83mg/ml creatine phosphate kinase) in a 1.5ml microcentrifuge tube (final concentration ∼7.4×106 nuclei/ml). This mixture was incubated at room temperature for 1h with agitation every 15 min. By the end of this period, 0.1% nuclei decondensed and egg membrane precursor vesicles (MVs) were bound to the chromatin. Excess MVs were discarded after centrifugation of nuclei through 30 μl of TN containing 0.5M sucrose in a 1.5ml tube at 750g for 15 min at 4°C. The pellet was processed for electron microscopy.
Nuclear envelope assembly in a cell-free system
0.1% L. pictus nuclei were thawed as above and after centrifugation were resuspended in 450 μl SXN buffer, supplemented with 10mM MβCD or vehicle control. Samples were incubated for 30 min at room temperature with periodic agitation followed by centrifugation at 1500g, 2 min, 4°C and resuspension in 50 μl TN buffer. MVs were bound to control nuclei as described in the previous section (untreated), or nuclei treated with 10mM MβCD. After 1h extracts were supplemented with 1mM GTP to induce fusion of MVs, or left unsupplemented as a negative control. After 2 hr, excess MVs were removed as above and nuclei were stained with 0.015mg/ml DiOC6 and viewed under a 100× oil immersion objective. DiOC6 was excited with the 488nm line of an Argon/Krypton laser, and the resulting fluorescence was separated using a combination of a dichroic beamsplitter (Q495LP; Chroma) and a HQ510/20nm emission filter. Alternatively, decondensed nuclei were observed with phase contrast. Images were captured with a Hamamatsu Orca camera and processed in OpenLab. Twenty nuclei were scored for NE formation on three independent occasions on the basis of their having a continuous fluorescent rim, and the mean and SEM of these results calculated.
Transmission electron microscopy (TEM)
For TEM, sperm or 0.1% nuclei were washed once in SXN and centrifuged at 500g, 4°C for 10 min or 1500g, 4°C for 2 min respectively. Pellets were fixed for 1 h in 0.1M Sorensen's buffer (81mM Na2HPO4, 19mM NaH2PO4 in distilled water, pH 7.4) containing 2.5% (v/v) glutaraldehyde and 1% (w/v) tannic acid. The pellet was washed in Sorensen's buffer by centrifugation at 500g for 10 min at 4°C. Samples were post-fixed in 1% (v/v) osmium tetroxide in 0.05M Sorensen's buffer for 30 min, washed and dehydrated in an ascending ethanol series and embedded in Araldite over 2 days. Thin sections of approximately 80nm were cut and observed on a JEOL 1010 TEM.
Cryo-TEM of nuclear envelope remnants
The method was adapted from Möbius et al. . The 0.1% nuclei were fixed in 4% (v/v) formaldehyde in 0.1M PHEM (60mM PIPES, 25mM HEPES, 2mM MgCl2, 10mM EGTA, pH 6.9) for 30 min on ice and infiltrated with 1.7M sucrose containing 4% (v/v) formaldehyde. Droplets containing sperm cells were put on cutting pins and frozen in liquid nitrogen. After cryo-sectioning, sections were picked up and thawed according to Liou et al.  in a 1∶1 mixture of 2.3M sucrose and 2% (v/v) methylcellulose.
Colorimetric determination of total phospholipids and cholesterol/cholesteryl ester (sterols)
Phospholipid concentration was determined by indirect measurement of inorganic phosphates liberated from extracted lipids. This method was adapted from Rouser et al. . Extracted lipids (see section “Mass spectrometry analysis”) were dissolved in 500 μl of TN buffer and transferred to glass test tubes. 500 μl of TN, water blank and K2HPO4 standard solutions ranging from 1 μM to 400 μM were transferred to glass test tubes. 100 μl of 10N sulfuric acid were added to each tube and the tubes were heated for 1.5h at 200°C. 100 μl of 72% (v/v) perchloric acid were added to each tube and the tubes heated for 60 min. 2ml of a distilled water/6N sulfuric acid/2.5% ammonium molybdate (w/v)/10% ascorbic acid (w/v) (7∶1∶1∶1 volume ratio) solution were added to the samples and they were incubated at 50°C for 30 min. Absorption was measured at 820nm. Cholesterol and cholesteryl ester concentrations were determined from lipid-extracted samples. Lipid pellets resuspended in 5 μl TN buffer and 5 μl of cholesterol standard solutions ranging from 0.3 to 7.8mM were supplemented with 500 μl of cholesterol liquid stable reagent provided by Thermo Electron Corporation. The solutions were probe-sonicated (Soniprep 150) at power 10 for 3 seconds and incubated for 5 minutes at 37°C. Absorption was measured at 500nm. From these assays the relative amounts of cholesterol to total lipids was determined. In the text, mol% refers to relative amount of cholesterol compared to total lipids including cholesterol.
Fluorescence imaging of 0.1% nuclei with filipin III
0.1% nuclei were fixed in 4% (v/v) formaldehyde in SXN buffer for 2.5 h at 4°C. Nuclei were centrifuged at 200g for 10 min at 4°C and resuspended in 30 μl of PHEM buffer. Filipin solution was 50 μg/ml in PHEM buffer . 1×106 nuclei were incubated in 1ml of filipin solution. Staining was carried out on a roller at 4°C for 4h. Nuclei were centrifuged at 200g for 10 min at 4°C and washed twice in 500 μl PHEM buffer. Nuclei were resuspended in 60 μl of PHEM buffer and counted using a hemocytometer. Volumes were adjusted to obtain an equivalent concentration of nuclei in all samples. 5 μl of nuclei were supplemented with 45 μl of PHEM buffer containing the anti-fade DABCO (100mg DABCO in 1ml of PHEM buffer). Filipin was excited at 360nm using a mercury source (Nikon Ltd) combined with a DAPI excitation filter (Nikon Ltd). Fluorescently labelled nuclei were viewed on a modified TE 2000 inverted microscope (Nikon Ltd). Images were captured with a Hamamatsu Orca camera and processed in OpenLab.
Fluoresence labeling of nuclei with filipin III
Unfixed L. pictus 0.1% nuclei were either untreated or extracted with 10mM MβCD as above and stained with filipin solution as described in the previous section. After PHEM washes, nuclei were counted and sample volumes adjusted to give an equal concentration of nuclei per sample. 50 μl of sample was transferred to a quartz cuvette and excited at 360nm by an arc lamp at 75W. Emission spectra were obtained from 400–650nm in a QM6/2005 spectrofluorimeter (PTI) using Felix32 Analysis software V1.0. The emission peak at 479/480nm was measured, and values normalized to control reactions. Additionally, the autofluorescence of unstained nuclei was measured and subtracted from filipin stained nuclei.
Quantification of phospholipids by HPLC coupled to tandem mass spectrometry (HPLC-ESI-MS/MS)
Biological samples were extracted in silanized glassware according to a modified Folch procedure . 200 μl of sample were added to 4ml of acidified chloroform/methanol (2.5∶1). The mixture was probe-sonicated for 10sec. Samples were filtered through a 0.22 μm Durapore membrane and supplemented with 0.2 volumes of 0.2M K4EDTA pH 6.8 solution. The extracted samples were centrifuged at 800g for 15 min at 4°C. The organic phase was dried at 37°C under nitrogen. The lipid pellet was resuspended in 100 μl chloroform/methanol/water (5∶5∶1) and transferred to a 150 μl silanized insert. The lipids were dried under nitrogen and supplemented with 2 μg of phospholipid internal standards (DLPC, DLPE, DPPI, DLPA, DLPS, DLPG) and 3 μg of phosphoinositide internal standards (PtdIns(4)P, PtdIns(4,5)P2 and PtdIns(3,4,5)P3). Before use, lipids were resuspended in chloroform/methanol/water (90∶9.5∶0.5) (phase A). A comparison of un-extracted versus extracted internal standards indicated that the filtering step does not lead to a loss of lipids (data not shown).
Mass spectrometry lipid analysis was carried out on an API 3000 instrument equipped with an ESI source (Sciex/Applied Biosystems). Lipids were separated by HPLC prior to detection using a normal phase Luna silica (2) 3 μm column (Phenomenex). A gradient elution protocol, adapted from Pettitt et al. , from 100% phase A containing 7mM ethylamine to 70% phase B1 [chloroform/acetonitrile/methanol/water (30∶30∶35∶5) containing 10mM ethylamine] over 20 min, was used to separate the phospholipids (PC, PG, PE, PI, PS and PA). The phosphoinositides were separated using a 100% phase A to 55% phase B2 [choroform/acetonitrile/methanol/water (30∶30∶32∶8) containing 10mM ethylamine] gradient over 20 min. Phospholipids were ionized at 4kV at 300°C. Phosphoinositide ionization was carried out under the same conditions except that the temperature was decreased to 150°C. Phospholipid species were determined using precursor scans: +184m/z for PtdCho, −196m/z for PtdEth, −241m/z for PtdIns, neutral loss of 87m/z for PtdSer and −153m/z for PtdAc. The collision energies were respectively: +52V, −45V, −60V, −35V and −40V. In a typical lipid analysis, precursor scans of phospholipids and multiple ion scans of phosphoinositides were acquired.
Fluoresence labelling of whole sperm and 0.1% nuclei with MARCKS peptide
Whole sperm were concentrated at 500g for 10 minutes, all centrifugations were carried out at 4°C unless otherwise stated. 250 μl of concentrated sperm were resuspended in 10ml MPSW (454mM NaCl, 9.7mM KCl, 24.9mM MgCl2, 9.6mM CaCl2, 27.1mM MgSO4, 4.4mM NaHCO3, 10mM Tris-HCl, pH 8.0) and centrifuged at 2600g for 5 minutes. The pellet was resuspended in 1.5ml Ca2+-free artificial seawater (454mM NaCl, 9.7mM KCl, 2.5mM NaHCO3, 25mM EGTA, pH 8.0). Alternatively 0.1% nuclei (previously cryogenically frozen) were thawed on ice and centrifuged at 1500g for 2 mins. The pellet was resuspended in 250 μl TN. 75 μl of either whole sperm or 0.1% nuclei sample was diluted in 425 μl Ca2+-free artificial sea water and Texas Red MARCKS effector domain peptide added to an end concentration of 120nM. The sample was incubated at room temperature in the dark for 30 minutes. Sperm/0.1% nuclei were collected by centrifugation at 1500g for 2 minutes and washed twice with Ca2+-free artificial seawater to remove excess MARCKS peptide. To visualise sperm/0.1% nuclei, 50 μl of sample was applied to a poly-lysine-coated coverslip and left for 5minutes to attach. Washing in Ca2+-free artificial seawater removed excess cells/nuclei. Whole sperm and 0.1% nuclei were viewed under a 100× oil immersion objective. Samples were excited with a mercury source and the resulting fluorescence was separated using a combination of a dichroic beamsplitter (D595DCLP; Chroma) and a HQ630/60nm emission filter. Images were captured with a Hamamatsu Orca camera and processed in OpenLab. Note that the MARCKS peptide is advantageous in these studies as polybasic peptides are cell permeant  allowing the analysis of live whole sperm to eliminate the possibility of fixation artefacts.
Deuterium-labelled vesicles for incorporation in natural membranes
This method was developed by Garnier-Lhomme et al. . Briefly, 0.1% nuclei were labelled with deuterated POPC small unilamellar vesicles (SUVs) obtained by probe-sonication above the lipid transition temperature for at least 15min using a 3mm microprobe for ultrahigh amplitude. The pulse cycles were a 4 sec sonication with a 6 sec interruption. SUVs were centrifuged at 10,000g for 10min in order to collect any metal residue from the probe that could interfere with NMR measurements. Median vesicle size was determined by dynamic light scattering to be 55nm. 5×109 frozen 0.1% nuclei were thawed on ice and centrifuged at 500g for 10min at 4°C. The pellets were resuspended in a total volume of 1ml of deuterium-depleted SXN buffer. Nuclei were centrifuged a second time as above and resuspended in 1ml of deuterium depleted SXN buffer to remove as much deuterated water as possible. For probe incorporation, a probe-to-nuclei lipid molar ratio of 0.6∶1 was used. The mixture was kept in a water bath at 40°C for 30 min and gently agitated every 10 min to avoid precipitation. The sample was transferred to a 10mm rotor.
Deuterium solid-state NMR spectroscopy
The 2H NMR spectra were acquired at 76.8MHz on a Bruker WB Avance DSX 500 (11.75T) spectrometer equipped with a static triple WB 1H/X/Y probe holding an in-house 10mm coil with 10 turns to increase sensitivity. Spectra were acquired by means of the quadrupolar echo pulse sequence 90°x-τ-90°y-τ-acq . The acquisition parameters for model membranes were as follows: spectral window of 500 kHz, π/2 pulse width 6.5 μs, recycle delay 1s and echo delay 30 μs. The number of acquisitions was 15k. Samples were allowed to equilibrate 45 minutes at a given temperature before the NMR signal was acquired. Typical experimental temperatures were 10°C for the initial acquisition, increased and equilibrated at 40°C and decreased to 10°C for the final acquisition. Carbon-deuterium order parameters, SCD, were calculated as detailed in  according to the equation:(1)ΔνQ is the quadrupolar splitting of the C–D bond considered. In non-oriented spectra, such as those of our study, the only measurable order parameter is that of the so-called “plateau” positions, i.e., labeled carbon positions from C2 to C10 and AQ the static quadrupolar splitting constant: AQ = 167kHz for paraffinic C–D bonds . Model membrane order parameter calculations were from Garnier-Lhomme et al. .
Nuclear envelope remnants line the acrosomal and centriolar fossae of 0.1% nuclei
To determine the morphology of nuclear envelope remnants we used transmission electron microscopy (TEM). Figure 1A shows a TEM image of a P. lividus sperm cell that was fixed in 2.5% (v/v) glutaraldehyde with 1% (w/v) tannic acid. The plasma membrane (PM) was osmotically swollen during sample preparation. The mitochondrial membranes (MM) and the nuclear envelope (NE) are well resolved. The remnant region is indicated by arrows.
(A) P. lividus sperm cells were fixed in the presence of 1% (w/v) tannic acid. The plasma membrane (PM) and the mitochondrial membranes (MM) are shown. AV: acrosomal vesicle, N: nucleus, F: flagellum. The nuclear envelope is tightly apposed to the chromatin but cup-like structures with nuclear envelope remnants can be seen at the poles (arrows). (B and C) S. purpuratus 0.1% nuclei were incubated in egg cytoplasm supplemented with ATP-GS and fixed in the presence of 1% (w/v) tannic acid. Electron dense structures (arrows) are shown in the centriolar (B) and acrosomal fossae (C). The two bilayers appear to have variable amounts of electron dense material between them. (D) Cryosections of S. purpuratus 0.1% nuclei prefixed in 4% (v/v) formaldehyde for 3h on ice show two membranes in the centriolar fossa (arrows). (E) S. purpuratus 0.1% nuclei were incubated in egg cytoplasm in the presence of an ATP-generating system, fixed in 2.5% (v/v) glutaraldehyde in the presence of 1% (w/v) tannic acid and viewed by TEM. The glancing cross section of the centriolar fossa shows the nuclear envelope remnants and an egg membrane vesicle (arrow) associated with the nuclear envelope remnants. Bars are 500nm (A), 400nm (B) and 200nm (C, D and E). The data are representative of nuclei observed in at least 3 experiments on independent nuclei preparations.
Figures 1B and C show 0.1% nuclei incubated in egg cytoplasm supplemented with ATP-GS and fixed with 1% (w/v) tannic acid present. Electron dense structures of ∼15nm thickness are shown in the centriolar (B) and acrosomal (C) fossae (arrows). Two bilayers are optimally resolved in certain regions where the central electron dense material is not uniform. Each bilayer was ∼6nm thick and may be continuous with each other at the ends. We refer to the structure as a double bilayer. These structures have very similar morphology to nuclear remnant membranes seen in vivo after fertilization . Electron dense structures of similar morphology have been particularly well resolved in octopus sperm . To acquire the images of the double bilayer in a less invasive manner, cryo-EM was used. S. purpuratus 0.1% nuclei were prefixed in 4% (v/v) paraformaldehyde, cryo-protected in 1.7M sucrose with 4% (v/v) formaldehyde and cryo-sectioned. Double membranes were also resolved and each was ∼7nm thick (arrows in Figure 1D).
S. purpuratus 0.1% nuclei were decondensed in egg cytoplasm with the ATP-GS and fixed in 2.5% (v/v) glutaraldehyde for TEM imaging. Figure 1E shows a section where the remnant lines the cupped shaped centriolar cavity with an egg membrane vesicle associated with the envelope remnant for reference (arrow). In places the double bilayer can be observed.
Membrane thickness in mammalian cells ranges from 4 to 7nm depending on the technique used – which agrees closely with our data. When stained with tannic acid, the two bilayers of nuclear envelope remnants were not always resolved clearly, probably due to the tannic acid interacting with either the headgroups of phosphatidylcholine  or to material between the parallel bilayers.
Both phospholipids and cholesterol localize at the nuclear envelope remnants
Given that nuclear envelope remnants are resistant to detergent solubilization, we predicted that they would be enriched in cholesterol. We therefore determined their cholesterol and cholesterol ester (sterol) content by colorimetric assay. Nuclear envelope remnants were 42±10 mol% sterol and 58±10 mol% total phospholipids. The relative amounts of sterols was higher than a typical mammalian plasma membrane of approximately 30 mol% cholesterol  but similar to the plasma membrane levels reported for the sea urchins A. punctulata (45%)  and L. variegatus (35%) . To show that the sterols were mainly localized at the nuclear envelope remnants, we labelled 0.1% nuclei with the sterol binding agent filipin. Figure 2 (top and middle left panels) shows two bright spots at both the acrosomal (middle panel) and centriolar fossae (top panel). The sterol signal was not concentrated in the chromatin. To ensure that phospholipids were also localized in the same regions as the sterols, we stained the demembranated nuclei with the lipophilic membrane dye, DiOC6 (Figure 2, bottom-left panel). The phospholipids were also located at both fossae, demonstrating that sterols are concentrated at the nuclear envelope remnants, not the chromatin.
The 0.1% nuclei were fixed in 4% (v/v) formaldehyde at 4°C and stained either with filipin to label cholesterol or DiOC6 to label nuclear envelope remnants lipids. Cholesterol labelling co-localizes with nuclear envelope remnants at both the acrosomal and centriolar fossae. DIC: differential interference contrast. Bars are 2.5 μm. Note that two panels of filipin staining are shown as in each case only one NER was in the confocal plane. Data are representative of at least 3 experiments performed on independent nuclei preparations.
Nuclear envelope remnants are enriched in polyunsaturated phosphoinositides
Once we had determined that the phospholipids and the sterols were at the site of the remnants, we analyzed the detailed phospholipid composition of the nuclear envelope remnants. For this analysis we exploited HPLC coupled to electrospray ionization tandem mass spectrometry (ESI-MS/MS).
The phospholipids of the 0.1% nuclei from L. pictus were extracted using a modified Folch method . Phospholipids separated by HPLC on a normal phase column were characterized by ESI-MS/MS using the precursor ion scans of sphingomyelin (SM), phosphatidylglycerol (PtdGly), phosphatidylethanolamine (PtdEth), phosphatidic acid (PtdAc), phosphatidylserine (PtdSer), phosphatidylcholine (PtdCho) and phosphatidylinositol (PtdIns) or using the multiple ion scans of phosphatidylinositolphosphate (PtdInsP), phosphatidylinositolbisphosphate (PtdInsP2) and phosphatidylinositoltrisphosphate (PtdInsP3). They were quantified relative to a mixture of internal standards.
Figure 3A shows the composition of the nuclear envelope remnants. Most striking is the high level of phosphoinositides and complete lack of sphingomyelin. Of total phospholipid content, the nuclear envelope remnants were 18±4 mol% PtdIns, 12±3 mol% PtdInsP, 12±2 mol% PtdInsP2 and 9±2 mol% PtdInsP3. The level of all PtdIns species together is 51%, much higher than the plasma membrane levels reported for A. punctulata (26%)  or cytoplasmic membranes in the sea urchin L. pictus (27%) . Therefore the remnants have the highest values of total PtdIns species reported for any natural membrane other than MV1 of L. pictus (60%) .
Lipid analysis of nuclear envelope remnants. (A) Lipids extracted from L. pictus demembranated sperm cells were separated by HPLC on a normal phase column and characterized by ESI-MS/MS using the precursor ion scans of sphingomyelin (SM), phosphatidylglycerol (PtdGly), phosphatidylethanolamine (PtdEth), phosphatidic acid (PtdAc), phosphatidylserine (PtdSer), phosphatidylcholine (PtdCho), phosphatidylinositol (PtdIns) or using the multiple ion scans of phosphatidylinositolphosphate (PtdInsP), phosphatidylinositolbisphosphate (PtdInsP2) and phosphatidylinositoltrisphosphate (PtdInsP3). Phospholipids were quantified using 12∶0/12∶0 (SM, PtdGly, PtdEth, PtdAc, PtdSer and PtdCho) or 16∶0/16∶0 (PtdIns, PtdInsP, PtdInsP2 and PtdInsP3) internal standards. Data expressed as mean±SEM (n = 3). (B) Alkyl-acyl versus diacyl phosphoinositides species distribution in nuclear envelope remnants. Mole percentages of diacyl species (green) and alkyl-acyl species (blue) were quantified from the multiple ion scans for each phosphoinositide class: PtdInsP, PtdInsP2 and PtdInsP3. 38% of PtdInsP, 15% of PtdInsP2 and 49% of PtdInsP3 are diacyl species. The PtdInsP2 is predominantly alkyl-acyl phosphoinositide. Data expressed as mean±SEM (n = 3).
Quantification of diacyl (Figure 3B-green) and alkyl-acyl (Figure 3B-blue) species from the multiple ion scans of the phosphoinositides showed that 38±4% of PtdInsP, 15±4% of PtdInsP2 and 49±14% of PtdInsP3 were diacyl lipids. Note that PLCγ prefers diacyl PtdIns(4,5)P2 as its substrate  and L. pictus MV1 vesicles highly enriched PLCγ are 76% diacyl PtdInsP2 whereas PtdInsP2 of the nuclear envelope remnants is predominantly alkyl-acyl. Also shown in Table 1, the major species of PtdCho, PtdEth and PtdIns were almost all polyunsaturated with the dominant species of each being arachidonyl (20∶4).
To corroborate our mass spectrometry lipid data we determined the localization of poly-phosphoinositide lipids on 0.1% nuclei. This was undertaken with a Texas Red MARCKS peptide containing a polybasic series of residues that form non-specific electrostatic interactions with all negatively charged phospholipids, but preferentially with the more highly phosphorylated phosphoinositides. The MARCKS peptide will sequester PtdIns(4,5)P2 preferentially over PtdSer, even when the latter is 300-fold in excess . Figure 4 shows the probe bound specifically to the regions of 0.1% nuclei corresponding to the NERs. This indicated that the poly-phosphoinositides detected in the ESI-MS/MS lipid analysis were localized in the NERs, and not within the chromatin region. The nuclei of eukaryoates are known to contain pools of phosphoinositides distinct from the lipids of the nuclear envelope , . To rule out that the NER phosphoinositide enrichment was not an artifact created by a preferential retention of nuclear matrix phosphoinositides during Triton extraction, we stained live whole sperm with Texas Red MARCKs. The peptide bound preferentially to the region of the NERs in over 80% of stained sperm examined. These data together indicate that NERs are enriched in poly-phosphoinositides in both live whole sperm and 0.1% nuclei. Furthermore, the lipid composition of NERs is not artificially created by detergent extraction.
L. pictus 0.1% nuclei (left) and whole live sperm (right) were incubated with the Texas Red labelled MARCKS peptide and visualised by fluorescence microscopy. The punctate staining of the acrosomal and centriolar fossae is typical of the majority of nuclei observed in experiments on two independent sperm and 0.1% nuclei preparations.
Probing microfluidity of nuclear envelope remnants by deuterium solid-state NMR spectroscopy
Given the lipid composition, we next addressed the dynamics of these membranes so high in cholesterol and polyphosphoinositide content. Our initial prediction was that the high levels of cholesterol would cause high molecular order and thus the nuclear envelope remnants would be relatively rigid membranes. To verify this hypothesis we used a method that would directly assess the order parameter of the nuclear envelope remnants.
We previously developed a deuterium solid-state NMR spectroscopy method for direct measurements of membrane dynamics . We used 16∶0(2H31)/18∶1 PtdCho (POPC-2H31) small unilamellar vesicles (SUVs) to deliver deuterated phosphatidylcholine and assess the dynamics of the nuclei devoid of lateral membranes but containing nuclear envelope remnants. Briefly, 0.1% nuclei (7×108 nuclei, 0.5 μmol lipids) were incubated with 0.29 μmol POPC-2H31 SUVs for 30 minutes at 40°C. Parallel experiments were performed with SUVs alone to show that the quadrupolar splitting variations were not due to the fusion of POPC-2H31 SUVs with each other.
Figure 5 (top panel) shows the POPC-2H31 SUV spectrum acquired at 10°C following treatment at 40°C for 22h. The isotropic peak, characteristic of small objects, was unchanged after treatment, demonstrating that the SUVs did not fuse to one another at higher temperatures. The panel below shows the 2H solid-state NMR spectrum of POPC-2H31 multilamellar vesicles (MLV) containing 30 mol% cholesterol acquired at 10°C. The broader quadrupolar splitting is referred to as “plateau” quadrupolar splitting and ΔνQ plateau (arrows of Figure 5) corresponds to the most restricted C–D bonds located close to the lipid glycerol backbone (labelled carbon positions C2 to C10). The ΔνQ plateau of MLVs containing 0 mol%, 15 mol% and 30 mol% cholesterol were respectively 28.9kHz, 35.9kHz and 45.0kHz (Table 2). Such values also indicate that the lipids are in a lamellar phase (40)
The top solid-state deuterium NMR spectrum corresponds to POPC-2H31 small unilamellar vesicles (SUV) acquired at 10°C post equilibrium at 40°C for 22h. The second spectrum corresponds to POPC-2H31 MLVs containing 30 mol% cholesterol acquired at 10°C after equilibration at 40°C. The bottom two spectra are of 0.1% nuclei incubated with SUVs of deuterated lipid (POPC-2H31) for 30 min at 40°C. Labelled nuclear envelope remnants were equilibrated at 40°C for 22h and the 2H NMR spectrum was acquired post-equilibrium at 10°C for 4h (NER Tfin1) and 20h (NER Tfin2). The dashed lines show the plateau quadrupolar splitting enlargement of labelled nuclear envelope remnants post-equilibrium. Arrows show the plateau quadrupolar splittings used to calculate the order parameters. NMR spectra are representative of those obtained in a duplicate experiment.
Labelled nuclear envelope remnants were first equilibrated at high temperature (40°C for 22h) and a spectrum acquired post-equilibrium at 10°C for 4h (Figure 5, “NER Tfin1”). Plateau quadrupolar splittings were not measured in this spectrum due to the low signal-to-noise ratio. To obtain a higher signal to noise ratio, a spectrum was also acquired after 20 hours (Figure 5, “NER Tfin2”). The isotropic peak was cropped to illustrate the powder pattern and represented about 10–15% of the total area. It was assigned to natural abundance 2H2O and/or to non-fused SUVs. Comparison of both spectra indicates that spectrum labeled “NER Tfin1” had not reached equilibrium. The observed spectrum has an unusual shape suggesting a distribution of “plateau” quadrupolar splittings ranging from 42 to 30 kHz (see dashed vertical lines).
The values of the ΔνQ plateau of nuclear envelope remnants suggest that the phospholipids were in a lamellar structure. The plateau quadrupolar splittings on the nuclear envelope remnants spectrum post-equilibrium (Tfin2), were used to calculate the order parameter (2SCD). This parameter ranged between 0.67 and 0.49, characteristic of the liquid-ordered phase, as generally promoted in the presence of sterols . The order parameter and the ΔνQ plateau of the nuclear envelope remnants were compared to model membranes with variable cholesterol contents treated in the same way (Table 2) . From this data and because the ordering promoted by cholesterol has been shown to be quasi linear with cholesterol concentration in membrane models  it can be estimated that the nuclear envelope remnant molecular order would correspond to model membranes with a cholesterol content ranging from 5 to 25 mole%. Since cholesterol esters induce ca. 20% less ordering on membranes  the amount of cholesterol esters would be ranging from 6 to 30 mole% if the whole sterol pool was only made of cholesterol esters. To sum up on the observed average ordering, the membrane is more disordered than a model membrane containing more than 40% cholesterol and cholesterol esters.
Sterol depletion of nuclei inhibits nuclear envelope formation in a cell free system
The data presented describe nuclear envelope remnants as double membrane structures with a unique lipid composition that are relatively fluid in nature. The 42 mole% sterol content of nuclear envelope remnants is closest to that of cortical vesicles (57%) . Given the role of cholesterol in facilitating cortical vesicle fusion to the plasma membrane  we hypothesized that cholesterol in the nuclear envelope remnants may play a similar role in promoting fusion events of nuclear envelope assembly. To test this, we treated 0.1% nuclei with 10mM MβCD, a sterol depleting agent , . Detection of sterols with filipin after MβCD treatment of nuclei indicated that there was considerably less sterols present, as indicated by a decrease of over 30% in filipin fluorescence intensity, compared to untreated nuclei (Figure 6A). Moreover, the same MβCD treated nuclei were less able to support nuclear envelope assembly in a cell free system (Figure 6B). In the presence of a fertilized egg cytoplasmic extract and ATP nuclei decondense to a spherical mass and bind egg MVs which fuse in the presence of GTP to form a nuclear envelope. Binding of MVs to MβCD treated nuclei was not affected but the ability of the MVs to subsequently fuse was inhibited by 35% (Figure 6B). Together, these indicate a functional role for nuclear envelope remnant sterols in the fusion events of nuclear envelope formation.
(A) 0.1% L. pictus nuclei were treated with 10mM MβCD or untreated (control). Nuclei were filipin stained, normalized to an equal nuclei concentration and excited at 360nm. The fluorescence intensity of the emission peak at 479/480nm was measured, and data were normalized to the control value. Data shown are mean±SEM of four experiments conducted in duplicate. (B) 0.1% L. pictus nuclei were treated with 10mM MβCD (white) or untreated (black). Nuclear envelope precursor MVs were subsequently bound to nuclei (ATP), and in parallel reactions their fusion was triggered with GTP to complete envelope formation. At least 20 nuclei were scored for the presence of a fully-formed nuclear envelope in 3 independent experiments. Data shown are mean±SEM.
The nuclear envelope remnants lining the acrosomal and centriolar fossae are natural membranes which have not been created due to artifacts of detergent treatment since they are present in untreated sperm. They reincorporate into the newly forming male pronuclear envelope in vivo and in vitro, are required for binding of nuclear envelope precursor membranes from the egg and are evolutionarily conserved , , . We have confirmed by TEM that the nuclear envelope remnants contain two membrane bilayers and demonstrated their lamellar structure by solid-state NMR spectroscopy. Despite their high sterol content, the membranes are more fluid than corresponding models, suggesting that other molecules in NERs counteract the ordering effect produced by sterols.
Although rich in sterols, nuclear envelope remnants are devoid of sphingomyelin, contain very polyunsaturated phospholipids and are strikingly enriched in polyphosphoinositides and arachidonyl phospholipids. Moreover, the fatty acids are mostly saturated on the sn1 position and polyunsaturated on the sn2 (Table1). Studies on model membranes, where the sn1 and sn2 are saturated and unsaturated respectively, have shown that cholesterol is intercalated parallel to the phospholipids , . Considering the above we speculate that cholesterol function may be to minimize steric and charge repulsions in the bilayer by intercalating between the large polyphosphoinositide headgroups and promote the liquid ordered phase to increase membrane stability. It is of note that Triton X-100 extraction has been proposed to alter the composition of model membranes in vitro . We believe this to be highly unlikely for NERs however, as the in vivo detection of phosphoinositides with the MARCKS effector domain indicated their enrichment in both live whole sperm and derergent extracted sperm. This is contrary to what one would expect if NERs were a detergent artefact only.
The elevated quantities of polyvalent phospholipids we observe in natural membranes lead to an obvious question of how these lipids might be distributed in the membrane bilayers. At the plasma membrane, lipid distribution is asymmetric with the polyphosphoinositides, present at much lower levels than other phospholipids, and mainly located on the inner leaflet . In the case of nuclear envelope remnants with high levels of polyphosphoinositides, the membrane would be unlikely to be so asymmetric and the phosphoinositides might be distributed on both sides of the bilayer to reduce but not eliminate charge repulsion. Some of the repulsion may be reduced by targeting cytoplasmic PH domain-containing proteins  and/or proteins containing basic/hydrophobic clusters such as the MARCKS proteins (Figure 4) and some small GTPases ,  to the PtdInsP2 and PtdInsP3. The attraction of positive amino acid residues would contribute to neutralizing the highly charged lipids. Likewise, the bilayer of nuclear envelope remnants closer to the chromatin might also have a more symmetric distribution of phosphoinositides with sterols stabilizing the charged lipids and chromatin proteins like histones neutralizing them.
We suggest that the unexpected fluidity of the nuclear envelope remnants results from the dominance of polyunsaturated lipids such as arachidonyl (20∶4) lipids in the phospholipid profile that decrease the ordering effect of high sterol concentration. These could be dispersed on both the cytoplasmic and nucleoplasmic adjacent bilayers. It has been shown that elevated quantities of polyunsaturated PtdIns enhance membrane fluidity . The “detergent resistant” properties of the remnants may be due to the association of the membrane with various proteins or chromatin attachment  and to the presence of the liquid-ordered state.
The work reported here leads to a possible explanation for the roles of enriched sterols and polyphosphoinositides in the nuclear envelope remnants. In the sea urchin, the fusion partners of the remnants, vesicles called MV1, are highly enriched in PLCγ and polyphosphoinositides , but not sterols [unpublished data]. Indeed they are the only other reported natural membranes containing more than 50% of their phospholipids as phosphoinositides. We have proposed that the role of activated PLCγ in MV1 is to produce from PtdInsP2 diacylglycerol, a lipid known to promote negative curvature and hence initiate localized membrane fusion . Indeed, experimentally elevating DAG in MV1 promotes NE formation . In contrast, the nuclear envelope remnants lack PLCγ or its preferred diacyl PtdIns(4,5)P2 substrate [unpublished data and ]. Instead they are enriched in alkyl-acyl PtdIns(4,5)P2 so in the absence of enzyme or substrate, generation of diacylglycerol from PtdIns(4,5)P2 in the remnants is unlikely.
Cyclodextrin depletion of sterols to ∼28 mole% typical of most plasma membranes  lead to partial inhibition of nuclear envelope formation. We suggest that for nuclear envelope assembly to occur, the sterols in the NERs might be needed to stabilize non-lamellar phases induced by the localized generation of DAG from MV1 to facilitate membrane fusion . The alkyl-acyl PtdIns(4,5)P2 in the nuclear envelope remnants, rather than being a source of DAG as in MV1, might instead serve to target proteins with polybasic clusters such as some GTPases which could regulate membrane fusion , .
In summary the properties and composition of the remnants and MV1 suggests that polyphosphoinositides and sterols, in addition to their many other functions , are present in a high mole fraction in some membranes to alter membrane structure so as to facilitate localized fusion. Investigations of the biophysical properties of membranes with similar composition to nuclear envelope remnants should give more direct insight into the roles of these lipids and their involvement in the molecular mechanisms of membrane fusion.
The authors thank Stuart McLaughlin for the Texas Red MARCKS peptide, scientific discussions and reading the manuscript, Fabrice Dumas for manuscript comments, Ken Blight, Trung Huynh, Christine Schmidt, Axelle Grélard, Cécile Loudet, and Vanessa Zhendre for helping with sample preparations, NMR and electron microscopy experiments and Paul Davies for his continuous support in supplying sea urchins to the Cell Biophysics Laboratory.
Conceived and designed the experiments: MGL RB RW DP ED BL. Performed the experiments: MGL RB TMH. Analyzed the data: MGL RB DP ED BL. Contributed reagents/materials/analysis tools: SG ED. Wrote the paper: MGL RB TMH DP ED BL.
- 1. Longo FJ, Anderson E (1968) The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata. J Cell Biol 39: 339–368.
- 2. Collas P, Poccia DL (1996) Conserved binding recognition elements of sperm chromatin, sperm lipophilic structures and nuclear envelope precursor vesicles. Eur J Cell Biol 71: 22–32.
- 3. Colwin AL, Colwin LH (1961) Changes in the spermatozoon during fertilization in Hydroides hexagonus (Annelida). II. Incorporation with the egg. J Biophys Biochem Cytol 10: 255–274.
- 4. Collas P, Poccia D (1995) Lipophilic organizing structures of sperm nuclei target membrane vesicle binding and are incorporated into the nuclear envelope. Dev Biol 169: 123–135.
- 5. Cameron LA, Poccia DL (1994) In vitro development of the sea urchin male pronucleus. Dev Biol 162: 568–578.
- 6. Larijani B, Poccia D (2007) Protein and lipid signaling in membrane fusion: nuclear envelope assembly. Signal Transduction 7: 142–153.
- 7. Collas P, Poccia D (1996) Distinct egg membrane vesicles differing in binding and fusion properties contribute to sea urchin male pronuclear envelopes formed in vitro. J Cell Sci 109 ( Pt 6): 1275–1283.
- 8. Franklin L (1965) Morphology of gamete membrane fusion and of sperm entry into oocytes of the sea urchin Journal of Cell Biology 25: 81–100.
- 9. Brown DA, London E (1998) Functions of lipid rafts in biological membranes. Annu Rev Cell Dev Biol 14: 111–136.
- 10. Radeva G, Sharom FJ (2004) Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells. Biochem J 380: 219–230.
- 11. Mukherjee S, Maxfield FR (2004) Membrane Domains. Annu Rev Cell Dev Biol 20: 839–866.
- 12. Brown DA (2006) Lipid rafts, detergent-resistant membranes, and raft targeting signals. Physiology (Bethesda) 21: 430–439.
- 13. Aussenac F, Tavares M, Dufourc EJ (2003) Cholesterol dynamics in membranes of raft composition: a molecular point of view from 2H and 31P solid-state NMR. Biochemistry 42: 1383–1390.
- 14. Ahn A, Gibbons DL, Kielian M (2002) The fusion peptide of Semliki Forest virus associates with sterol-rich membrane domains. J Virol 76: 3267–3275.
- 15. Ablan S, Rawat SS, Viard M, Wang JM, Puri A, et al. (2006) The role of cholesterol and sphingolipids in chemokine receptor function and HIV-1 envelope glycoprotein-mediated fusion. Virol J 3: 104.
- 16. Churchward MA, Rogasevskaia T, Hofgen J, Bau J, Coorssen JR (2005) Cholesterol facilitates the native mechanism of Ca2+-triggered membrane fusion. J Cell Sci 118: 4833–4848.
- 17. Chen Z, Rand RP (1997) The influence of cholesterol on phospholipid membrane curvature and bending elasticity. Biophys J 73: 267–276.
- 18. Kooijman EE, Chupin V, Fuller NL, Kozlov MM, de Kruijff B, et al. (2005) Spontaneous curvature of phosphatidic acid and lysophosphatidic acid. Biochemistry 44: 2097–2102.
- 19. Coorssen JR, Rand RP (1990) Effects of cholesterol on the structural transitions induced by diacylglycerol in phosphatidylcholine and phosphatidylethanolamine bilayer systems. Biochem Cell Biol 68: 65–69.
- 20. Churchward MA, Rogasevskaia T, Brandman DM, Khosravani H, Nava P, et al. (2008) Specific lipids supply critical negative spontaneous curvature–an essential component of native Ca2+-triggered membrane fusion. Biophysical journal 94: 3976–3986.
- 21. Byrne RD, Garnier-Lhomme M, Han K, Dowicki M, Michael N, et al. (2007) PLCgamma is enriched on poly-phosphoinositide-rich vesicles to control nuclear envelope assembly. Cell Signal 19: 913–922.
- 22. Collas P, Poccia DL (1995) Formation of the sea urchin male pronucleus in vitro: membrane-independent chromatin decondensation and nuclear envelope-dependent nuclear swelling. Mol Reprod Dev 42: 106–113.
- 23. Collas P, Courvalin JC, Poccia D (1996) Targeting of membranes to sea urchin sperm chromatin is mediated by a lamin B receptor-like integral membrane protein. J Cell Biol 135: 1715–1725.
- 24. Byrne RD, Barona TM, Garnier M, Koster G, Katan M, et al. (2005) Nuclear envelope assembly is promoted by phosphoinositide-specific phospholipase C with selective recruitment of phosphatidylinositol-enriched membranes. Biochem J 387: 393–400.
- 25. Gambhir A, Hangyas-Mihalyne G, Zaitseva I, Cafiso DS, Wang J, et al. (2004) Electrostatic sequestration of PIP2 on phospholipid membranes by basic/aromatic regions of proteins. Biophys J 86: 2188–2207.
- 26. Garnier-Lhomme M, Dufourc EJ, Larijani B, Poccia DL (2007) Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin. Methods in molecular biology: lipid technology and signaling: In press.
- 27. Mobius W, Ohno-Iwashita Y, van Donselaar EG, Oorschot VM, Shimada Y, et al. (2002) Immunoelectron microscopic localization of cholesterol using biotinylated and non-cytolytic perfringolysin O. J Histochem Cytochem 50: 43–55.
- 28. Liou W, Geuze HJ, Slot JW (1996) Improving structural integrity of cryosections for immunogold labeling. Histochem Cell Biol 106: 41–58.
- 29. Rouser G, Fkeischer S, Yamamoto A (1970) Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5: 494–496.
- 30. Mukherjee S, Zha X, Tabas I, Maxfield FR (1998) Cholesterol distribution in living cells: fluorescence imaging using dehydroergosterol as a fluorescent cholesterol analog. Biophys J 75: 1915–1925.
- 31. Larijani B, Poccia DL, Dickinson LC (2000) Phospholipid identification and quantification of membrane vesicle subfractions by 31P-1H two-dimensional nuclear magnetic resonance. Lipids 35: 1289–1297.
- 32. Pettitt TR, Dove SK, Lubben A, Calaminus SD, Wakelam MJ (2006) Analysis of intact phosphoinositides in biological samples. J Lipid Res 47: 1588–1596.
- 33. Takeuchi T, Kosuge M, Tadokoro A, Sugiura Y, Nishi M, et al. (2006) Direct and rapid cytosolic delivery using cell-penetrating peptides mediated by pyrenebutyrate. ACS Chem Biol 1: 299–303.
- 34. Garnier-Lhomme M, Grélard A, Byrne RD, Loudet C, Dufourc EJ, et al. (2007) Probing the Dynamics of Intact Cells and Nuclear Envelope Precursor Membrane Vesicles by Deuterium Solid State NMR Spectroscopy. Biochim Biophys Acta 1786: 2516–2527.
- 35. Davis J, Jeffrey K, Bloom M, Valic M, Higgs T (1976) Quadrupolar echo deuteron magnetic resonance spectroscopy in ordered hydrocarbon chains. Chemical Physics Letters 42: 390–394.
- 36. Burnett LJ, Müller BH (1971) Deuteron quadrupolar coupling constants in three solid deuterated paraffin hydrocarbons: C2D6, C4D10, C6D14. J. Chem. Phys. 55: 5829–5831.
- 37. Ribes E, Gimenez-Bonafe P, Martinez-Soler F, Gonzalez A, Saperas N, et al. (2004) Chromatin organization during spermiogenesis in Octopus vulgaris. I: Morphological structures. Mol Reprod Dev 68: 223–231.
- 38. Franke WW (1974) Structure, biochemistry, and functions of the nuclear envelope. Int Rev Cytol Suppl 4: 71–236.
- 39. Mitra K, Ubarretxena-Belandia I, Taguchi T, Warren G, Engelman DM (2004) Modulation of the bilayer thickness of exocytic pathway membranes by membrane proteins rather than cholesterol. Proc Natl Acad Sci U S A 101: 4083–4088.
- 40. McCaughan L, Krimm S (1980) X-ray and Neutron Scattering Density Profiles of the Intact Human Red Blood Cell Membrane. Science 207: 1481–1483.
- 41. Hochmuth RM, Evans CA, Wiles HC, McCown JT (1983) Mechanical measurement of red cell membrane thickness. Science 220: 101–102.
- 42. Kalina M, Pease DC (1977) The preservation of ultrastructure in saturated phosphatidyl cholines by tannic acid in model systems and type II pneumocytes. J Cell Biol 74: 726–741.
- 43. Maxfield FR, Wustner D (2002) Intracellular cholesterol transport. J Clin Invest 110: 891–898.
- 44. Kinsey WH, Decker GL, Lennarz WJ (1980) Isolation and partial characterization of the plasma membrane of the sea urchin egg. The Journal of cell biology 87: 248–254.
- 45. Decker SJ, Kinsey WH (1983) Characterization of cortical secretory vesicles from the sea urchin egg. Developmental biology 96: 37–45.
- 46. Irvine RF (2003) Nuclear lipid signalling. Nat Rev Mol Cell Biol 4: 349–360.
- 47. Irvine RF (2006) Nuclear inositide signalling–expansion, structures and clarification. Biochim Biophys Acta 1761: 505–508.
- 48. Beck JG, Mathieu D, Loudet C, Buchoux S, Dufourc EJ (2007) Plant sterols in “rafts”: a better way to regulate membrane thermal shocks. Faseb J 21: 1714–1723.
- 49. Leonard A, Dufourc EJ (1991) Interactions of cholesterol with the membrane lipid matrix. A solid state NMR approach. Biochimie 73: 1295–1302.
- 50. Chana RS, Cushley RJ, Wassall SR, Smith ICP, Dufourc EJ (1985) Organization of Cholesteryl Esters in Membranes-a Deuterium Nuclear Magnetic-Resonance Study. Chem Phys Lipids 37: 345–356.
- 51. John K, Kubelt J, Muller P, Wustner D, Herrmann A (2002) Rapid transbilayer movement of the fluorescent sterol dehydroergosterol in lipid membranes. Biophys J 83: 1525–1534.
- 52. Roche Y, Gerbeau-Pissot P, Buhot B, Thomas D, Bonneau L, et al. (2008) Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts. Faseb J.
- 53. Brzustowicz MR, Stillwell W, Wassall SR (1999) Molecular organization of cholesterol in polyunsaturated phospholipid membranes: a solid state 2H NMR investigation. FEBS Lett 451: 197–202.
- 54. Rand RP, Luzzati V (1968) X-ray diffraction study in water of lipids extracted from human erythrocytes: the position of cholesterol in the lipid lamellae. Biophys J 8: 125–137.
- 55. Sot J, Bagatolli LA, Goni FM, Alonso A (2006) Detergent-resistant, ceramide-enriched domains in sphingomyelin/ceramide bilayers. Biophys J 90: 903–914.
- 56. McLaughlin S, Wang J, Gambhir A, Murray D (2002) PIP(2) and proteins: interactions, organization, and information flow. Annu Rev Biophys Biomol Struct 31: 151–175.
- 57. Gray A, Van Der Kaay J, Downes CP (1999) The pleckstrin homology domains of protein kinase B and GRP1 (general receptor for phosphoinositides-1) are sensitive and selective probes for the cellular detection of phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 3,4,5-trisphosphate in vivo. Biochem J 344 Pt 3: 929–936.
- 58. Golebiewska U, Gambhir A, Hangyas-Mihalyne G, Zaitseva I, Radler J, et al. (2006) Membrane-bound basic peptides sequester multivalent (PIP2), but not monovalent (PS), acidic lipids. Biophys J 91: 588–599.
- 59. Heo WD, Inoue T, Park WS, Kim ML, Park BO, et al. (2006) PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membrane. Science 314: 1458–1461.
- 60. Larijani B, Dufourc EJ (2006) Polyunsaturated phosphatidylinositol and diacylglycerol substantially modify the fluidity and polymorphism of biomembranes: a solid-state deuterium NMR study. Lipids 41: 925–932.
- 61. Barona T, Byrne RD, Pettitt TR, Wakelam MJ, Larijani B, et al. (2005) Diacylglycerol induces fusion of nuclear envelope membrane precursor vesicles. J Biol Chem 280: 41171–41177.
- 62. Wang W, Yang L, Huang HW (2007) Evidence of Cholesterol Accumulated in High Curvature Regions: Implication to the Curvature Elastic Energy for Lipid Mixtures. Biophys J.
- 63. Di Paolo G, De Camilli P (2006) Phosphoinositides in cell regulation and membrane dynamics. Nature 443: 651–657.