Optimizing locked nucleic acid/2’-O-methyl-RNA fluorescence in situ hybridization (LNA/2’OMe-FISH) procedure for bacterial detection

• Andreia S. Azevedo,

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Writing – original draft

  Affiliations CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal, LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, IPATIMUP - IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

  http://orcid.org/0000-0002-0521-2841

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• Inês M. Sousa,

  Roles Investigation, Methodology

  Affiliation LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal

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• Ricardo M. Fernandes,

  Roles Investigation, Methodology

  Affiliations CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal, LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal, INIAV, IP- National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão, Vila do Conde, Portugal

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• Nuno F. Azevedo,

  Roles Funding acquisition, Project administration, Supervision, Validation, Writing – review & editing

  Affiliation LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal

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• Carina Almeida

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Validation, Writing – review & editing

  * E-mail: carina.almeida@iniav.pt

  Affiliations CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal, LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal, INIAV, IP- National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão, Vila do Conde, Portugal

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