PONE-D-24-01082Saturated Transposon Analysis in Yeast as a One-step Method to Quantify the Fitness Effects of Gene Disruptions on a Genome-Wide ScalePLOS ONE

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Reviewer #1: Yes

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Reviewer #1: I Don't Know

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: #Review Report#

Dear Editor.

Thank you for the opportunity given to me to review this manuscript titled “Saturated Transposon Analysis in Yeast as a One-step Method to Quantify the Fitness Effects of Gene Disruptions on a Genome-Wide Scale” for publication in your PLOS ONE Journal. I have reviewed the manuscript and found it quite interesting. The manuscript is well-composed. The dataset therein is technically sound. There is enough justification to affirm the originality of the work done; the numerical figures of the various analytical factors have been reported with a reasonable degree of accuracy. The aim of the work, as outlined in the introductory part and illustrated in Figure 1 of the manuscript has been sufficiently achieved. On the general note, the work has underscored the critical role of saturated transposon analysis in quantification of fitness effect of gene disruption on a genome-wide scale, which could undeniably help in advancing the knowledge of functional genomics, particularly in the construction of genetic interaction maps. In my opinion, the manuscript has met both the scientific and ethical standards for inclusion in the publication of scientific record. However, I have outlined few comments below that could help improve the quality of the manuscript.

Comments:

1. P2/36. L75. The word “the” Consider removing one.

2. P6/36. L235. The word figure should be written with initial capital letter to make it consistent with the other usage in the manuscript.

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Reviewer #1: No

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Saturated Transposon Analysis in Yeast as a One-step Method to Quantify the Fitness Effects of Gene Disruptions on a Genome-Wide Scale

PONE-D-24-01082R1

Dear Dr. LAAN,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Bashir Sajo Mienda, PhD

Academic Editor

PLOS ONE

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