PONE-D-21-07398

A Green-lipped mussel reduces pain behavior and chondrocyte inflammation and attenuated experimental osteoarthritis progression

PLOS ONE

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: I Don't Know

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: No

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5. Review Comments to the Author

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Reviewer #1: The paper by JooYeon Jhun et al, describes the anti-inflammatory and joint-protecting properties of green-lipped mussel in a in vivo model of MIA.

The rational behind the experiment was clear and straight forward. The manuscript is almost well written

I recommend that the paper be accepted with minor revision:

a) The authors should better check the manuscript for any typographical errors.

b) The authors should clarify in the Materials and methods section the experimental groups and the experimental conditions.

c) The discussion does a good job at explaining the importance of the results in the context of the inflammatory pathways involved. However, incorporation of previous results from other related studies with natural compound is lacking such as 10.3390/ani10101827 or 10.3390/antiox9060511 or 10.1186/s13075-016-1189-5 or 10.1186/s13075-019-2048-y or 10.1155/2021/5534614 or 10.3390/antiox10020265

d) please provide high magnification of figure 2,3,4 and 5 to better understand the signal maybe as supplementary files and better clarify the method for IHC quantification

Reviewer #2: The manuscript by JooYeon Jhun al ” A Green-lipped mussel reduces pain behavior and chondrocyte

inflammation and attenuated experimental osteoarthritis progressionnts”

The manuscript is interesting in its field, showing an overview of the GLM use in osteoarthritis progressionnts.

I recommend major revision for the paper:

• The authors should improve the english language

• The author should update the bibliography. For example in the introduction the author should insert several paper about the anti inflammatory and antioxidative application of compound present in diet(doi.org/10.3390/antiox9060511;oi.org/10.1186/s13075-019-2048-y)

• the authors should indicate the experimental design through a temporal sequence, clearly indicating the administrations and tests carried out

• The authors should increase the quality of the images, for example in figure 2C, the photos appear to have been taken in different places

• Is there a mechanism of action for GLM? The authors speak of a mediated NfkB mechanism, has a mechanism directly linked to GLM been hypothesized?

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Reviewer #1: No

Reviewer #2: No

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A Green-lipped mussel reduces pain behavior and chondrocyte inflammation and attenuated experimental osteoarthritis progression

PONE-D-21-07398R1

Dear Dr.

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Rosanna Di Paola, MD

Academic Editor

PLOS ONE

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