Peer Review History

Original SubmissionAugust 26, 2019
Decision Letter - Jeffrey Chalmers, Editor

PONE-D-19-24047

Circulating cell free DNA during definitive chemo-radiotherapy in non-small cell lung cancer patients – initial observations

PLOS ONE

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PLOS ONE

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Reviewers' comments:

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Comments to the Author

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: • The data demonstrate that there was no concordance between radiological response and cfDNA change during treatment. This in line with previous observations which have shown a lack of predictive accuracy for total cfDNA (Weiss G et al, Clin Cancer Res 2017; 23: 5074-81). The paper of Weiss, however, demonstrated that on the other hand chromosomal instability quantification in plasma cfDNA was associated with therapeutic response to immune therapy. This paper should be mentioned in the publication.

• P. 6: The authors state that samples were analyzed “… using sWGS, resembling the method of plasma-Seq” and give a citation. Some more detailed information on how they applied this method should perhaps be given in the supplement.

• To what extent was the sensitivity of somatic copy number alterations detection increased?

• P. 9: The authors state that the mean increase in cfDNA was different in AC and SCC patients. Was this difference statistically significant?

• P. 11: What was the detectable ctDNA fraction with their method? In this context, the authors could cite a paper of Fiala et al (J Appl Lab Med 2018; 03: 300-313) which provides information on the likelihood of ctDNA detection related to detectable fraction.

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Reviewer #1: Yes: Michael Oellerich

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Revision 1

Thank you very much for the revisions of our manuscript. The comments have been addressed in "Response to reviewers"

Attachments
Attachment
Submitted filename: Response to reviewers.docx
Decision Letter - Jeffrey Chalmers, Editor

Circulating cell free DNA during definitive chemo-radiotherapy in non-small cell lung cancer patients – initial observations

PONE-D-19-24047R1

Dear Dr. Nygård,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication.

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With kind regards,

Jeffrey Chalmers, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Formally Accepted
Acceptance Letter - Jeffrey Chalmers, Editor

PONE-D-19-24047R1

Circulating cell free DNA during definitive chemo-radiotherapy in non-small cell lung cancer patients – initial observations

Dear Dr. Nygård:

I am pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please notify them about your upcoming paper at this point, to enable them to help maximize its impact. If they will be preparing press materials for this manuscript, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

For any other questions or concerns, please email plosone@plos.org.

Thank you for submitting your work to PLOS ONE.

With kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Dr. Jeffrey Chalmers

Academic Editor

PLOS ONE

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