PONE-D-20-01523

Fecal shedding of Salmonella spp., Clostridium perfringens, and Clostridioides difficile in dogs fed raw meat-based diets in Brazil and their owners’ motivation

PLOS ONE

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PLOS ONE

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[This work was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Council for Scientific and Technological Development (CNPq), the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and the Pró-Reitoria de Pesquisa da UFMG (PRPq/UFMG).]

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 [This worked was supported by the Minas Gerais Research Support Foundation

(Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG), the

Brazilian Federal Agency for the Support and Evaluation of Graduate Education

(Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior - CAPES), the

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Reviewers' comments:

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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5. Review Comments to the Author

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Reviewer #1: Relevance of Salmonella in foods should be adressed ( Food Control, Volume 104, October 2019, Pages 308-312; Int J dairy technology, Volume73, Issue1, February 2020, Pages 296-300; Journal of Dairy ScienceVolume 102, Issue 8August 2019Pages 6756-676).

The author should add more pratical consideration about the study,

The English is adaequate too.

Please decrease the introduction also.

Reviewer #2: The manuscript provides actual information regarding the use of processed feed against raw food in pets and the potential risk of zoonotic bacterial transmission and spread. Besides the useful information about how the owners understand the risks associated with raw meat and they care about this issue, the manuscript provides novel information about phenotypic and genetic characteristics of Salmonella, C. perfringens and C. difficile in feed and pets. According to the vision of this reviewer, the manuscript adds a valuable and useful information to the specific literature.

Writing style should be carefully reviewed. Please see that some time you use Clostridium difficile and others Clostridioides difficile. Also, there are several empty spaces before punctuations introduced during typing that should be corrected.

Reviewer #3: The authors present results mainly about important enteropathogens such as Salmonella spp., C. perfringens, and C. difficile, in feces of dogs fed different diets, raw meat-based diets (RMBD) or commercial dry feed. The topic is interesting. The abstract and the introduction are good and show all information about the work. The presented data support the conclusions

My observations are the next:

check the spaces

Line 110- specify times and conditions

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Reviewer #1: No

Reviewer #2: Yes: Mariano Fernandez-Miyakawa

Reviewer #3: No

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Fecal shedding of Salmonella spp., Clostridium perfringens, and Clostridioides difficile in dogs fed raw meat-based diets in Brazil and their owners’ motivation

PONE-D-20-01523R1

Dear Dr. Silva,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication.

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With kind regards,

Anderson de Souza Sant'Ana, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: