Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N. lirii sp. nov.

Slow growing oomycete isolates with morphological resemblance to Phytophthora were obtained from forest streams during routine monitoring for the EU quarantine forest pathogen Phytophthora ramorum in Ireland and Northern Ireland. Internal Transcribed Spacer (ITS) sequence analysis indicated that they belonged to two previously unknown species of Nothophytophthora, a recently erected sister genus of Phytophthora. Morphological and temperature-growth studies were carried out to characterise both new species. In addition, Bayesian and Maximum-Likelihood analyses of nuclear 5-loci and mitochondrial 3-loci datasets were performed to resolve the phylogenetic positions of the two new species. Both species were sterile, formed chlamydospores and partially caducous nonpapillate sporangia, and showed slower growth than any of the six known Nothophytophthora species. In all phylogenetic analyses both species formed distinct, strongly supported clades, closely related to N. chlamydospora and N. valdiviana from Chile. Based on their unique combination of morphological and physiological characters and their distinct phylogenetic positions the two new species are described as Nothophytophthora irlandica sp. nov. and N. lirii sp. nov. Their potential lifestyle and geographic origin are discussed.


Introduction
Nothophytophthora is a monophyletic sister genus of Phytophthora, and was erected in 2017 to accommodate several slow growing previously unknown oomycete species recovered from surveys of rhizosphere soil and streams in forest habitats in Europe, Asia and South America [1][2][3]. The main features differentiating Nothophytophthora from other closely related oomycete genera are the presence of a conspicuous, opaque plug inside the sporangiophore close to the base of most mature sporangia in all known Nothophytophthora species and intraspecific co-occurrence of caducity and non-papillate sporangia with internal nested and extended proliferation in several Nothophytophthora species. Jung et al. [1] described six species within the genus Nothophytophthora. Isolates of other potentially novel Nothophytophthora taxa have been isolated by several research groups during the last decade [4][5][6][7]. Ireland has a heavily modified landscape, with over 60% of the land cover devoted to agricultural grassland [8]. The natural vegetation of the island of Ireland would consist mostly of temperate deciduous forests [9], although at present just 11% of the land area is forested [10]. Consequently, the majority of research in plant pathology in Ireland has been focussed on agricultural pathogens. The diversity of oomycetes in natural and semi-natural habitats on the island of Ireland, comprising the Republic of Ireland and the UK country Northern Ireland, has not been well studied. O'Hanlon et al. [11] presented evidence for the presence of 27 species of Phytophthora, and speculated that at least a further 11 species probably remained to be found based on species records from the UK. Surveys of forests, horticultural premises, and public horticultural gardens in the past five years have produced first records of eight Phytophthora species and several other oomycete species previously unrecorded in Ireland [12,13].
In recent surveys of Irish and Northern Irish habitats for the EU regulated forest pathogen Phytophthora ramorum, collection and testing of Rhododendron leaves from wild plants and from water baits in streams revealed several oomycete isolates which morphologically resembled Phytophthora [12][13][14]. Preliminary ITS sequence analysis indicated that these slow growing isolates belonged to two previously unknown species of Nothophytophthora. In this study, morphological and physiological characteristics were used in combination with multigene phylogenetic analyses to characterise the two new Nothophytophthora taxa, compare them with the known species of Nothophytophthora, and officially describe them as Nothophytophthora irlandica sp. nov. and Nothophytophthora lirii sp. nov.

Ethics statement
This study was performed within the frame of the annual surveys of Irish and Northern Irish habitats for the EU quarantine forest pathogen P. ramorum. The surveys were funded by, and had oversight from, the National Plant Protection Organisations of both jurisdictions. No specific permissions were required. Our field sampling did not involve endangered or protected species.

Isolate collection and maintenance
Baiting was performed in two and one streams in Ireland and Northern Ireland, respectively, (Fig 1) using young leaves of Rhododendron ponticum or Rhododendron caucasicum × ponticum 'Cunningham's White' as baits in mesh sacs floating on the water [12,13]. The baiting in the Ow stream in Ireland took place between early 2014 and late 2015, with a total of 10 baits being tested during that period. The baiting in the Shimna stream in Northern Ireland took place between mid-2017 and early 2018, with a total of 7 baits being tested. In addition, attached leaves with lesions of plants of R. ponticum near the Owenashad and Shimna streams were collected on each occasion and tested. Furthermore, naturally fallen necrotic leaves of R. ponticum and other hosts (e.g. Fagus sylvatica, Fraxinus excelsior, Quercus petraea, Corlyus avellana) floating in two streams in Ireland and one stream in Northern Ireland were also sampled [3]. Collections in the Owenashad stream in Ireland were conducted in March and December 2014; in August 2015; in July 2017; and in March, June, July and August 2018. Collections in the Shimna stream in Northern Ireland happened in February, April, June, July, Isolations from necrotic areas of baiting leaves and naturally fallen leaves were performed using selective P5ARP agar (cornmeal agar with antibiotics; [15]). For all isolates, single hyphal tip cultures were produced under the stereomicroscope from the margins of fresh cultures on V8-juice agar (V8A; 16 g agar, 3 g CaCO 3 , 100 ml Campbell's V8 juice, 900 ml distilled water). Stock cultures were maintained on grated carrot agar (CA; 16 g agar, 3 g CaCO 3 , 200 g grated carrots, 1000 ml distilled water; [16,17]) at 10˚C in the dark. All isolates of the two new Nothophytophthora spp. are preserved in the culture collections maintained at the Agri-Food and Biosciences Institute, Belfast, Northern Ireland, and the culture collection maintained at Mendel University in Brno, Czech Republic. Ex-type and additional cultures were deposited in the CBS culture collection at the Westerdijk Fungal Biodiversity Institute (previously Centraalbureau voor Schimmelcultures CBS; Utrecht, The Netherlands). Details of all isolates used in the phylogenetic, morphological and temperature-growth studies are given in Table 1.
GenBank numbers for sequences obtained in the present study are printed in italics; extype isolates are printed in bold-type; t.s., this study;-, not available.

DNA isolation, amplification and sequencing
For all Nothophytophthora isolates obtained in this study and for two isolates each of the six described Nothophytophthora species the Phire Plant Direct Master Mix (Thermo Fisher Scientific Inc., Gloucester, UK) was applied for direct PCR from fresh pure cultures growing on V8A, following the manufacturer's instructions. The mycelium extract diluted in dilution buffer was stored at -20˚C. For N. irlandica and N. lirii five nuclear and three mitochondrial loci were amplified and sequenced. The internal transcribed spacer region (ITS1-5.8S-ITS2) of the ribosomal RNA gene (ITS) and the 5' terminal domain of the large subunit (LSU) of the nuclear ribosomal RNA gene (nrDNA) were amplified separately using the primer-pairs ITS1/ITS4 [18] and LR0R/LR6-O [19,20]. Partial heat shock protein 90 (hsp90) gene was amplified with the primers HSP90F1int and HSP90R1 as described previously [21]. Segments of the β-tubulin (btub), the mitochondrial genes cytochrome c oxidase subunit 1 (cox1), and NADH dehydrogenase subunit 1 (nadh1) genes were amplified with primers TUBUF2 and TUBUR1, COXF4N and COXR4N, FM84 and FM85, and NADHF1 and NADHR1, respectively, using the PCR reaction mixture and cycling conditions as described earlier [22,23]. Partial rps10 gene was amplified according to the protocol provided by OomyceteDB (http:// oomycetedb.cgrb.oregonstate.edu/protocols.html) using primer pair rps10_DB-FOR and rps10_DB-REV. Partial tigA gene amplification was performed using primers Tig_FY and G3PDH_rev according to Blair et al. [21]. For the six described Nothophytophthora species only rps10 and tigA were amplified. All amplicons were purified and sequenced in both directions by Eurofins Genomics GmbH (Cologne and Ebersberg, Germany) using the primers of the PCR reactions except for the tigA amplicons for which primers Tig_rev and G3PDH_for were used [21]. Electropherograms were quality checked and forward and reverse reads were compiled using Geneious Prime v. 2021.0.3 (Biomatters Ltd., Auckland, New Zealand). Clearly visible pronounced double peaks were considered as heterozygous positions and labelled according to the IUPAC coding system. All sequences derived in this study were deposited in GenBank and accession numbers are given in Table 1.

Phylogenetic analysis
The sequences obtained in this work for N. irlandica, N. lirii and the six described Nothophytophthora species were complemented with sequences of the latter retrieved from GenBank [1]. The sequences of the loci used in the analyses were aligned using the online version of MAFFT v. 7 [24] by the E-INS-I strategy (ITS) or the G-INS-I strategy (all other loci). To analyse the phylogenetic positions of N. irlandica and N. lirii within the genus Nothophytophthora a 5-partition dataset (5,492 characters) of the nuclear loci ITS, LSU, btub, hsp90 and tigA and 3-partition dataset (1,762 characters) of the mtDNA genes cox1, nadh1 and rps10 were established. All analyses included five isolates of N. irlandica, 10 isolates of N. lirii, two isolates each of the six known Nothophytophthora species and Phytophthora rubi (CBS 967.95) as outgroup taxon. With both datasets Bayesian (BI) analyses were performed using MrBayes 3.1.2 [25,26] into partitions with the invgamma model. Four Markov chains were run for 20 M generations, sampling every 1,000 steps, and with a burn in at 8,000 trees. In addition, Maximum-Likelihood (ML) analyses were carried out using the raxmlGUI v. 2.0 [27] implementation of RAxML [28] with a GTR+G nucleotide substitution model. There were 10 runs of the ML and bootstrap ("thorough boostrap") analyses with 1,000 replicates used to test the support of the branches. Phylogenetic trees were visualized in MEGA X [29] and edited in figure editor programs. Datasets presented and trees deriving from Maximum likelihood and Bayesian analyses are available from TreeBASE (27579; http://purl.org/phylo/treebase/phylows/study/TB2: S27579).

Morphology of asexual and sexual structures
Formation of sporangia was induced by submersing two 12-15 mm square discs cut from the growing edge of a 3-7 d old V8A colony in a 90 mm diam Petri dish in non-sterile soil extract (50 g of filtered oak forest soil in 1000 ml of distilled water, filtered after 24 h; [30]). The Petri dishes were incubated at 20˚C in natural light and the soil extract was changed after 6 h [31]. Shape, type of apex, caducity and special features of sporangia and the formation of hyphal swellings were recorded after 24-48 h. For each isolate 40 sporangia and 25 zoospore cysts were measured at ×400 using a compound microscope (Zeiss Imager.Z2), a digital camera (Zeiss Axiocam ICc3) and a biometric software (Zeiss ZEN). The formation of chlamydospores and hyphal swellings was examined on V8A after 15-30 d growth at 20˚C in the dark. For each isolate 40 chlamydospores and hyphal swellings chosen at random were measured under a compound microscope at ×400 [1,31].
The formation of gametangia (oogonia and antheridia) and their characteristic features were examined after 21-30 d growth at 20˚C in the dark on a carrot agarose medium [32]. Isolates from both new taxa were also paired with A1 and A2 tester strains of P. ramorum using the method of Brasier and Kirk [33] and with A1 and A2 tester strains of P. cinnamomi using the method of Jung et al. [31].

Colony morphology, growth rates and cardinal temperatures
Colony growth patterns of both Nothophytophthora species were described from 14-d-old cultures grown at 20˚C in the dark in 90 mm plates on CA, V8A and potato dextrose agar (PDA; Oxoid Ltd., UK) [31,34,35]. For temperature-growth relationships, five and nine isolates of N. irlandica and N. lirii, respectively, were subcultured onto 90 mm V8A plates and incubated for 24 h at 20˚C to stimulate onset of growth [31]. Then three replicate plates per isolate were transferred to 10,15,20,25,26,27,28,29 and 30˚C. Radial growth was recorded after 6 d, along two lines intersecting the centre of the inoculum at right angles and the mean growth rates (mm/d) were calculated. To determine the lethal temperature, plates showing no growth at 26, 27, 28, 29 or 30˚C were re-incubated at 20˚C.

Nomenclature
The electronic version of this article in Portable Document Format (PDF) in a work with an ISSN or ISBN will represent a published work according to the International Code of Nomenclature for algae, fungi and plants, and hence the new names contained in the electronic publication of a PLOS ONE article are effectively published under that Code from the electronic edition alone, so there is no longer any need to provide printed copies. In addition, new names contained in this work have been submitted to MycoBank from where it will be made available to the Global Names Index. The unique MycoBank number can be resolved and the associated information viewed through any standard web browser by appending the MycoBank number contained in this publication to the prefix http://www.mycobank.org/MB/. The online version of this work is archived and available from the following digital repositories: PubMed Central, LOCKSS. For the nuclear 5-partion dataset the BI analysis provided higher support for the deeper nodes than the ML analysis (Fig 2). Nothophytophthora irlandica and N. lirii were closely related and formed a fully supported clade which clustered in sister position to N. valdiviana. Within N. lirii the three isolates from a tributary of the Shimna River in Northern Ireland (CBS 147244, P18 27A, P18 27C) constituted a distinct, well supported subclade. Nothophytophthora chlamydospora resided in a strongly supported basal position to the N. irlandica-N. lirii-N. valdiviana cluster. This clade of four sterile species clustered in sister position to a clade comprising the three homothallic species N. amphigynosa, N. intricata and N. vietnamensis. The sterile species N. caduca resided in a basal position to these two clades.

Phylogenetic analysis
The BI and ML trees of the mitochondrial 3-partition dataset had a different topology compared to the nuclear 5-loci trees and showed character conflicts at deeper nodes indicated by low support values and a polytomy (Fig 3). Nothophytophthora irlandica and N. chamydospora  (Fig 4).
Oogonia, oospores and antheridia-All five isolates of N. irlandica examined were self-sterile and did not form gametangia in single culture or in pairings with A1 and A2 tester strains of P. ramorum and P. cinnamomi.
Colony morphology, growth rates and cardinal temperatures (Figs 5 and 6)-Colonies of the five tested isolates of N. irlandica on V8A and CA were appressed to submerged and had either rosaceous or faintly striate to uniform patterns. On PDA colonies of all isolates were appressed and dense felty with a more or less clear rosaceous pattern and irregular margins (Fig 5). All five isolates of N. irlandica included in the temperature-growth test had similar growth rates and cardinal temperatures. The maximum and lethal growth temperatures were 25 and 30˚C, respectively (Table 2, Fig 6). The average radial growth rate at the optimum temperature of 20˚C was 2.1 ± 0.3 mm/d ( Table 2, Fig 6).
Colony morphology, growth rates and cardinal temperatures (Figs 5 and 6)-Colonies showed slight variations between the nine isolates tested. On V8A and CA they were mostly faintly radiate with limited, appressed-felty aerial mycelium in the center and often with irregular and sometimes submerged margins. On PDA colonies were dense-felty white, sometimes with faint concentric rings and always with irregular margins which were partly submerged (Fig 5). Temperature-growth relations are shown in Fig 6. All nine tested isolates had similar growth rates and cardinal temperatures. The maximum and lethal growth temperatures were 25 and 30˚C, respectively. The average radial growth rate at the optimum temperature of 20˚C was 1.7 ± 0.3 mm/d (Table 2; Fig 6).

Notes
Nothophytophthora irlandica and N. lirii share many features with the six described Nothophytophthora species, including slow colony growth with relatively low maximum temperatures for growth and the production of a conspicuous opaque plug at the sporangial base. Both new Nothophytophthora species differ from N. amphigynosa, N. caduca, N. intricata, N. valdiviana and N. vietnamensis by having considerably slower growth at both 20˚C and 25˚C and, in addition, from N. intricata, N. valdiviana and N. vietnamensis by having a lower optimum temperature for growth (20˚C vs 25˚C) (Fig 6; [1]). In addition, they are easily distinguished from N. amphigynosa, N. intricata and N. vietnamensis by being sterile (Table 2; [1]). Nothophytophthora chlamydospora is phylogenetically closest to the two new Nothophytophthora species and shares with them the sterile breeding system and the production of chlamydospores and of partially caducous sporangia with exclusively external proliferation ( Table 2; [1]). However, N. irlandica and N. lirii can be distinguished from N. chlamydospora by having considerably slower growth at 15 and 20˚C and faster growth at 25˚C, by producing smaller sporangial sympodia (less than 4 sporangia vs less than 6-8 sporangia) and by the absence of secondary chlamydospores on hyphae radiating from primary chlamydospores. In addition, compared to N. chlamydospora, N. lirii and N. irlandica produce on average larger chlamydospores and longer sporangia, respectively. Nothophytophthora irlandica and N. lirii differ from each other in the sizes of their sporangia and chlamydospores and in their colony morphologies on V8A and CA (Table 2; Fig 5). Furthermore, N. irlandica and N. lirii formed well supported distinct clades in the BI and ML analyses of both the nuclear 5-loci and the mitochondrial 3-loci datasets.

Hosts and geographic distribution
Nothophytophthora irlandica and N. lirii have hitherto only been detected on R. ponticum leaves floating naturally or as baits in streams in Ireland and Northern Ireland. Naturally fallen  nonpapillate, ovoid, slighly laterally attached; c. nonpapillate, elongated-ovoid; d. ellipsoid, with swollen apex before zoospore release and with beginning external proliferation (arrow); e. nonpapillate, elongated-obpyriform with two basal plugs (arrow); f. nonpapillate, limoniform, on a short lateral hypha, with vacuole and external proliferation; g. nonpapillate, elongated-ellipsoid, curved, with two basal plugs (arrow); h. ovoid, with swollen apex before release of the fully differentiated zoospores, with beginning external proliferation; almost breaking-off at the basal plug (arrow); i. same ovoid sporangium as in g releasing zoospores; j. elongated-ovoid, caducous sporangium with short pedicel-like basal plug (arrow); k. secondary, lateral sporangium forming just below the empty upper section of the sporangiophore (arrow); l. dense sympodium with two empty sporangia after zoospore release and one immature limoniform sporangium; m-t. structures formed in solid V8 agar; m-s. globose or subglobose thick-walled chlamydospores; m. leaves of other tree species (e.g. Fraxinus, Fagus, Corylus, Quercus) floating in rivers at locations where the new Nothophytophthora species had been recovered never yielded any isolates of Nothophytophthora. Similarly, testing of symptomatic foliage from R. ponticum plants near two of these streams never yielded any isolates of Nothophytophthora. Several other oomycete species were recovered from the same streams, including Phytophthora gonapodyides, P. chlamydospora, P. lacustris, and Elongisporangium undulatum. Also, P. ramorum and P. cactorum were isolated from foliage of R. ponticum plants near the streams. Although several hundred leaves were tested for oomycetes only 15 isolates of N. lirii and N. irlandica were obtained during 2 of the 17 baiting occasions and 5 of the 15 sampling occasions of naturally fallen leaves. Therefore, neither of the two new Nothophytophthora species can be considered as being common in the watercourses surveyed.

Discussion
This study has shown that the unknown oomycete isolates from streams in Ireland and Northern Ireland constitute two new distinct Nothophytophthora species, described here as N. irlandica and N. lirii. Both new species were differentiated from the six known Nothophytophthora species and from each other based on morphological characteristics, temperature-growth relationships and multi-locus phylogenetic analyses. The nuclear and mitochondrial multi-loci trees had different topologies indicating different evolutionary histories of the nuclear and mitochondrial Nothophytophthora genomes. Discordances between mitochondrial and nuclear genealogies are common and usually caused by incomplete lineage sorting or mitochondrial introgression [36][37][38][39][40]. Nonetheless, N. irlandica, N. lirii and the six known Nothophytophthora species formed in the BI and ML analyses of both the nuclear and mtDNA multi-locus datasets eight distinct strongly supported clades.
In the original description of the genus Nothophytophthora Jung et al. [1] pointed out that despite numerous oomycete surveys being carried out each year across the globe, sequences of just three strains at GenBank were matching Nothophytophthora. Two of these strains are designated here as ex-type isolates of N. irlandica (Pr13-109 = CBS 147242) and N. lirii (Pr12-475 = CBS 147293). A third strain, named "Phytophthora sp. REB326-69", was isolated from a stream in Huia in New Zealand [5] and its sequence (GenBank accession JX122744) showed 99% similarity to N. chlamydospora and N. valdiviana [1] and also to N. irlandica and N. lirii. Additional btub sequence screening of isolates derived from stream baiting in northern New Zealand between 2008 and 2010 [4] revealed 17 isolates in the N. irlandica-N. lirii clade (Gen-Bank accessions MW542641-MW542657). Further characterisation of two of these isolates with cox1 sequences (GenBank accessions MW542639 and MW542640) determined that they were N. irlandica. Nothophytophthora caduca, N. chlamydospora and N. valdiviana were described from the Valdivian region in Chile while N. amphigynosa, N. intricata and N. vietnamensis were first detected in Portugal, Germany and Vietnam, respectively [1]. In recent global surveys, using classical baiting tests or metabarcoding approaches, both described and unknown Nothophytophthora taxa were infrequently detected. These  [7] and Spain [43]. In addition, LSU, btub and cox1 sequences recently submitted to GenBank (e.g. accession nos. for isolate SM08APR_ANG1: MG685808, terminal; n. subglobose, intercalary inserted; o. laterally sessile; p-q. terminal with a few swollen, radiating hyphae (arrows); r-s. intercalary inserted; t. obpyriform sporangium that ailed to form a basal septum and continued to grow at the apex. Scale bar = 25 μm, applies to a-t.
https://doi.org/10.1371/journal.pone.0250527.g007 MG701979, MG701951) show that N. caduca occurs in Californian streams, more than 10,000 km distant from the original findings in Chile [1]. Apparently, despite their occurrence in most continents, members of the genus Nothophytophthora are only infrequently found in oomycete surveys. The most likely explanation for the scarcity of Nothophytophthora records is their slow growth in culture preventing their isolation in the presence of faster growing oomycete genera, i.e. Elongisporangium, Pythium, Phytopythium and Phytophthora [1]. In the temperature-growth test of this study both N. irlandica and N. lirii showed even slower growth than the six known Nothophytophthora species. Thus, their consistent isolation over consecutive years from the same streams in Ireland and Northern Ireland, despite the presence of the much faster growing oomycetes P. chlamydospora, P. gonapodyides, P. lacustris and E. undulatum, indicates competitive sustainable populations.
The question arises whether the two new Nothophytophthora species are native or nonnative to Ireland and Northern Ireland. The phylogenetic analyses of this study revealed that N. irlandica and N. lirii are closely related sister species of N. chlamydospora and N. valdiviana. Due to their close phylogenetic relatedness these four Nothophytophthora species must originate from the same biogeographic region, either Europe or temperate regions of South America. There are several lines of indirect evidence supporting that the species are non-native to the island of Ireland. The island of Ireland has no areas of pristine forests, with just 2% of the land area of Ireland classified as semi-natural native forests [44]. Of the total forest area of 673,000 ha, 68, 19 and 13% of the forests are composed of non-native, native or a mixture of non-native and native tree species, respectively [10]. Consequently, there are only few habitats in Ireland or Northern Ireland left undisturbed by human activities, including the inadvertent introduction of invasive plants and microorganisms to the wider environment. In recent years several Phytophthora species, including P. ramorum, P. lateralis and P. kernoviae were introduced to Irish habitats, most likely through the trade in plants-for-planting [11,45]. Phytophthora kernoviae has only been reported from the UK, Ireland, New Zealand and Chile [2,[46][47][48][49]. Phytophthora kernoviae most likely originates from the Valdivian rainforests of Chile [2]. Since both N. chlamydospora and N. valdiviana also co-occur in the same forests [1,2] it seems feasible that P. kernoviae, N. irlandica and N. lirii were all introduced from Chile to the island of Ireland, most likely on living plants. Analogous, also the populations of P. kernoviae and N. irlandica in New Zealand might have been introduced from Chile, either directly or via the UK and Ireland as steppingstones. However, population genetic analyses of Chilean, Irish, British and New Zealand populations of Nothophytophthora and P. kernoviae are needed to confirm this hypothesis. The limited distribution of Nothophytophthora species in streams on the island of Ireland also points to their non-native status, with other recent surveys for Phytophthora in Ireland failing to isolate Nothophytophthora species [12,13].
Oomycetes are increasingly emerging as one of the most significant threats to global plant health [50][51][52]. Since all known Nothophytophthora isolates were recovered from waterbodies or -less frequently -rhizosphere soil, it is important to clarify whether Nothophytophthora species are plant pathogens or saprotrophs. Aquatic saprotrophic oomycetes, in particular Phytophthora species, are usually characterised by high cardinal temperatures, fast growth, a sterile breeding system, thin-walled chlamydospores, and the abundant production of non-papillate persistent sporangia with internal proliferation [53,54]. Having very slow growth, low cardinal temperatures and partially caducous sporangia with infrequent or lacking internal proliferation, Nothophytophthora species do not fit the profile of competitive aquatic saprotrophs [1]. Instead, a partially aerial lifestyle as leaf and shoot pathogens had been proposed with stream populations resulting at least partly from canopy drip [1]. In the natural and seminatural forests in Chile, Vietnam and Portugal from which N. caduca, N. chlamydospora, N. valdiviana, N. amphigynosa and N. vietnamensis were isolated, no obvious symptoms of above-ground infections of plant tissues were noticed [1][2][3]. Likewise, in two of the streams where N. irlandica and N. lirii was present in Ireland and Northern Ireland, testing of attached symptomatic R. ponticum foliage did not reveal any Nothophytophthora species. Extensive ongoing tests of the potential aerial and soilborne pathogenicity and host ranges of the six known Nothophytophthora species, the two new Nothophytophthora species from Ireland and other yet undescribed Nothophytophthora species are currently being performed and their results will help to understand the lifestyle and pathological importance of Nothophytophthora species. Given that both of the species described here produce chlamydospores abundantly, and these structures are known to aid in survival of biologically unfavourable periods and in long-distance spread, the risk of these species spreading in plant trade should be assessed [55,56].