The influence of sample geometry and size on porcine aortic material properties from uniaxial tensile tests using custom-designed tissue cutters, clamps and molds

The aim of this study was to identify the influence of specimen geometry and size on the results of aortic uniaxial tensile tests using custom-designed tissue cutters, clamps and molds. Six descending thoracic aortas from pigs were used for rectangular sample tests, in which the circumferential and axial specimens had widths of 6 mm, 8 mm and 10 mm. The other six aortas were used for the dog-bone-shaped sample tests and were punched into circumferential, axial and oblique specimens with widths of 2 mm, 4 mm and 6 mm. We performed uniaxial tensile tests on the specimens and compared the test results. The results showed that mid-sample failure occurred in 85.2% of the dog-bone-shaped specimens and in 11.1% of the rectangular samples, which could be caused by Saint-Venant’s principle. Therefore, rectangular specimens were not suitable for aortic uniaxial tensile testing performed until rupture. The results


Introduction
Aortic rupture is an important cause of death in forensic practices with a mortality rate as high as 80%-94.4% [1][2][3].This phenomenon is often caused by internal diseases (such as aortic dissection [4,5], aneurysms [6][7][8], and atherosclerosis [9]) or blunt force [10][11][12] and can occur during cardiopulmonary resuscitation [13].It is difficult to identify the cause of rupture or the degree of participation of trauma when trauma and disease coexist.The biomechanical analysis of injury based on the finite element method is important when studying the mechanism of aortic injuries, and one of the key research bases is the material property data of the aorta.
Consequently, the reported experimental results are often incomparable, especially in regard to the failure stress and strain.Table 1 summarizes some of the previous studies documented in the literature regarding the failure stress and strain of intact artery walls.This table lists the source, geometry and size of the samples and provides the failure stress and strain values of the samples under uniaxial tensile load.This table is not a complete summary of all uniaxial rupture tests of intact artery wall samples published in the literature; however, it is intended to provide a representative overview that illustrates that failure stress and strain values are discrete.
Mohan and Melvin [28] performed pioneering research and measured circumferential and axial failure stresses and strains under quasi-static and dynamic conditions using dog-boneshaped samples that were 6.35 or 4.57 mm in width.The circumferential and longitudinal tensile strength values under quasi-static conditions were 1.7±0.2 and 1.5±0.2MPa, respectively.In studies using healthy thoracic aortas, Sherebrin et al. [14] reported lower values, of which the failure stresses in the circumferential and longitudinal directions were 177±104 kPa and 184±90 KPa, respectively, by using rectangular samples of 5 mm width.However, Garcı ´a-Herrera et al. [30] reported higher values, of which the values in circumferential and longitudinal directions were 2180±240 kPa and 1140±100 kPa, respectively.
In other studies [16,26,28,30,31], the circumferential failure stress was greater than the axial stress in both normal aortic walls and aortic aneurysm walls.However, a few studies [18,23] reported the opposite results, whereas Vorp et al. [17] found no significant difference in the different directions.The ultimate stress of the aortic aneurysm walls was found to be significantly lower in the two directions than that in the healthy aortic walls [16][17][18].Layer-specific characterization of human aortas has also been studied in recent years, and the results vary [34][35][36][37][38][39][40].
Therefore, it is still a challenge to find a scientific and reasonable experimental procedure to accurately characterize the mechanical properties of aortic tissues.The objective of this study is to identify the influence of specimen geometry and size and determine the optimal specimen by comparing the test results of specimens of different geometries and sizes processed by custom-designed tissue cutters, clamps and molds.

Specimen collection and initial processing
All aortic tissue specimens were obtained in accordance with the guidelines of the review board of the Academy of Forensic Science.Twelve descending thoracic aortas from fivemonth-old pigs were harvested from a local slaughterhouse (Shanghai Yunong Meat Products Co., Ltd., Shanghai) and immediately wrapped in saline-soaked gauze, after which they were stored in an ice-filled box for transport.The aortas reached the laboratory within two hours Note: ABA = abdominal aorta; AN = aneurysmatic, ASA = ascending aorta, BAV = bicuspid aortic valve, Circ = circumferential, DTA = descending thoracic aorta, Long = longitudinal, TAV = tricuspid aortic valve, and � = narrowed region. https://doi.org/10.1371/journal.pone.0244390.t001 after harvesting.All samples were frozen to -80˚C until experimental testing.Among them, 6 aortas were used for rectangular sample tests and the other 6 aortas were used for the dogbone-shaped sample tests.Before the test, the samples were removed from the refrigerator at -80˚C and immersed in normal saline without Ca 2+ at 4˚C overnight.Then, the loose connective tissue attached to the adventitia was carefully removed so that only the aortic wall itself remained for testing after equilibrating at room temperature.

Mechanical testing using rectangular samples
Six descending thoracic aortas were used for this test.Rectangular specimens that are 6 mm, 8 mm and 10 mm in width (the 6-mm-wide specimens were punched into a dog-bone shape, but they were clamped to the beginning of the narrow zone) were punched from the aortic wall in the circumferential and axial orientation with custom-designed tissue cutters, and the aortic ostia were avoided.Due to the large circumferential diameter at the proximal portion of the descending aortas, three circumferential tissue strips that are 6 mm, 8 mm and 10 mm in width were punched there.Below these strips, three longitudinal specimens were acquired (Fig 1A).Six specimens were taken from each aorta, and a total of 36 specimens were taken from 6 aortas.The samples with the same width and direction were divided into one group and named RC 6 , RC 8 , RC 10 , RL 6 , RL 8 and RL 10 .There were 6 specimens for each group.R indicated rectangular, C indicated circumferential, L indicated longitudinal, and the number indicated the width of the test area in mm.For example, RC 6 represented rectangular circumferential samples with a 6-mm-wide test area.Note that the specimens of each width should appear to be equal at each position in each segment.Table 2 shows a detailed description of the sample groups.
The circumferential samples were limited by the diameter of the aorta, which was approximately 50 mm, ensuring that the aspect ratio of the test area was 3:1 [21,22].The lengths of the axial samples were generally not less than 80 mm, ensuring that the aspect ratio of the test area was 4:1.The width and thickness of the samples were photographed vertically in the front and the side and measured at five locations in the test area using ImageJ software to obtain average values.The two ends of each strip were quickly sandwiched between the custom-designed clamps and coupled with cyanoacrylate while ensuring the unity of the aspect ratio on the custom-designed molds with the scale.The custom-designed cutters, clamps and molds used for specimen processing are shown in Fig 2A -2E.An Instron 5967 machine was used to perform uniaxial tests on the samples at room temperature.The clamps and samples were mounted in the jaws of the pneumatic grips of the testing machine.The distance between the grips was adjusted, starting from a configuration with some slack, and then the sample was slowly extended until the load cell recorded a tensile force of 0.01 N.That was assumed to be the load-free configuration (initial point).The distance between the grips in this initial point was the original length L 0 .The load and the grip displacement were measured by the testing machine, and the grip displacement was taken as a direct measurement of the elongation of the samples because the rest of the elements were much more rigid than the specimens.Each specimen was preconditioned by applying five cycles with a 20%×L 0 /min displacement rate at L 0 ×4% stretch to eliminate the hysteresis effect of tissues and obtain repeatable stress-strain curves.Then, tensile testing was carried out at the same speed until tissue failure.The applied force and corresponding displacement of the grips were collected synchronously and continuously at a sampling rate of 100 Hz until specimen failure.The specimens were kept wet by spraying saline solution before and during the experiment.

Mechanical testing using dog-bone-shaped samples
Six descending thoracic aortas were used for the test.The aortas were divided into three segments: proximal, middle and distal, with lengths of approximately 6 cm, 8 cm and 8 cm, respectively.The circumferential samples were obtained from the proximal section, the longitudinal section from the middle section, and the oblique 45˚samples from the distal section.In each of the three segments, customized cutters were used to punch out three dog-boneshaped samples, for which the dimensions of the narrow middle part were 12 mm×2 mm, 24 mm×4 mm and 30 mm×6 mm; moreover, note that the aortic ostia were avoided (Fig 1B).
Nine specimens were obtained from 1 aorta, and 54 specimens were obtained from 6 aortas.The specimens with the same width and direction were divided into a group and named DC 2 , DC 4 , DC 6 , DL 2 , DL 4 , DL 6 , DO 2 , DO 4 , and DO 6 .There were 6 specimens for each group.Note that D indicated dog-bone-shaped, C indicated circumferential, L indicated longitudinal, O indicated oblique, and number indicated the width of the test area.For example, DC 2 represented dog-bone-shaped samples with the circumferential direction, and the width of the test area was 2 mm.Note that the specimens of each width should appear to be equal at each position in each segment.The detailed description of the sample groups is shown in Table 2.
The measurements of the original width and thickness of the test area and the clamping of the specimens were consistent with the previous experiments.
Position diagram of samples on the aortic wall Water-proof black ink was applied to the inner side of the samples after the strips were clamped.Two white paint spots, which were spaced 4 times the width apart at the center of the narrow zone of the specimens, were tracked by the video extensometer to identify the original length L 0 and displacement of the test area (Fig 2F).If the samples were directly marked, it was difficult to recognize by the video extensometer because the color contrast was not obvious.
An Instron 5967 machine was used to perform the uniaxial tests.At the initial point, the distance between the two markers was the original length, L 0 .The applied force and corresponding displacement of the markers were collected synchronously and continuously at a sampling rate of 100 Hz.The other experimental procedures were consistent with the previous one.

Evaluation of the mechanical parameters
The applied force and the extension were recorded during each experiment.Combined with the width and thickness of the measured initial state of the specimens, engineering strain, engineering stress, real strain and real stress can be converted.The engineering strain (ε E ) is elongation (ΔL) divided by the initial length (L 0 ): The engineering stress (σ E ) is the applied load (F) divided by the initial cross-sectional area (A 0 ): The true strain (ε T ) is the natural log of the current length (L) divided by the initial length (L 0 ): The volume of the aortic wall was assumed to remain unchanged during the elongation process [41], so the current cross-sectional area A was obtained by the equation: The true stress (σ T ) is calculated as the applied load (F) divided by the current cross-sectional area (A).
Because the complex material constitutive model involves more parameter fitting and most of the parameters have no direct mechanical significance, it is not convenient for comparison between groups.To simplify the analysis and facilitate comparisons between the specimens in different groups, the mathematical model introduced by Raghavan et al. [16] was used in this study.The relationship between strain and stress is expressed as follows: where ε is the engineering strain, σ is the true stress, and K, A, and B are model parameters in the formula.According to the theory, the aorta can be simplified into containing two primary passive load bearing fibers: elastin and collagen.The stress-strain curve is divided into three phases (Fig 3).
In phase 1, the stress is low, and only the elastic fibers are taut (Fig 3 , phase 1).As σ !0 and B + σ � B, the formula for the Young's modulus of elastin (E E ) can be expressed as follows: In phase 2, the collagen fibers start contributing to load bearing, and the slope of the stressstrain curve increases gradually (Fig 3 , phase 2).Prior to tissue failure, the maximum slope of the final portion of the stress-strain curve corresponds to the combined total stiffness of the elastin and collagen fibers in phase 3 (Fig 3 , phase 3).Assume that B � σ, so that B + σ � σ, and the following formulas can be derived: where E C is the Young's modulus of collagen.For detail, as hown by Raghavan et al. [16], K is the inverse of (E E +E C ).A is the strain intercept of the final portion (phase 3) of the stress-strain curve (Fig 3 ), A is inversely proportional to the rate of recruitment of the collagen fibers for a given strain rate, or directly proportional to the average degree of tortuosity of the collagen fibers in the tissue.A can be considered the "recruitment parameter".The smaller A is, the faster the collagen fibers activate.B is the value of stress at the intersection of the lines defined by the linear responses in phase 1 and phase 3 (dashed and solid lines in Fig 3,respectively).Moreover, ε u and σ u are the ultimate strain and ultimate stress, respectively.
Thus, the stress-strain curve is simplified into three parameters to characterize the main mechanical properties of the aortic wall: K, A and B.Moreover, E E and E C can be deduced from these parameters.Compared with the use of more precise and complex material constitutive relations, this method of selecting parameters for comparison is more meaningful and easier to operate.

Data processing and statistics
The stress-strain curve was plotted, and the ultimate strain (ε u ) and ultimate stress (σ u ) were recorded for each specimen.Using the Levenberg-Marquardt for nonlinear regression (SPSS, Statistics, version 20), the mathematical model s was fit to the experimentally measured stress-strain data obtained for each specimen, yielding the best-fit parameters K, A, and B. E E and E C were deduced from these parameters for each specimen.According to the previous groups, the values of the parameters are presented as the mean ± standard deviation for each group.The groups were compared by one-way analysis of variance (ANOVA) followed by least significant difference (LSD) multiple comparison tests if the overall comparison was significant.A two-tailed independent Student's t-test was performed for the circumferential and axial specimens from the groups with a width of 6 mm between the rectangular and dog-bone-shaped groups examined in this study using SPSS v20.Significance was assumed for a p value less than 0.05.

Data fitting and comparison
The regression of all data sets converged, obtaining the best fitting parameter of each specimen.Fig 5 indicates the representative experimental data of the five groups and the corresponding fit of the model that was selected in the present study using the best fitting parameters.
The stress-strain curves for all the specimens indicated a satisfactory fit with the mathematical model, and the means of the parameters in the different groups are shown in Table 3. Fifteen model-generated stress-strain curves for each group, which were based on the mean values of the model parameters in Table 3, are shown in Fig 6.
For the rectangular circumferential specimens, the difference was in parameter B. The B value of RC 6 was significantly higher than the B values of RC 8 and RC 10 .However, for the rectangular axial specimens, none of the parameters showed significant difference.For the dogbone-shaped circumferential, axial and oblique specimens, the differences were in parameters A and B. The values of these parameters in the groups with 2-mm-wide specimens were significantly higher than the values in the other groups (Table 3).
The physical properties of each group, including the elastin and collagen fiber moduli (E E and E C ), ultimate strain (ε u ) and ultimate stress (σ u ), are shown in Table 4.
For the rectangular circumferential specimens, the difference was in E E .The E E value of RC 6 was significantly higher than the values of RC 8 and RC 10 .None of the four values showed significant difference for the rectangular axial specimens.For the dog-bone-shaped circumferential samples, the values of E E , ε u and σ u were different.The E E value of DC 2 was significantly higher than the values of DC 4 and DC 6 .Moreover, the values of ε u and σ u were significantly different among the three groups: the values of DC 2 were larger than those of DC 4 , and the values of DC 4 was larger than those of DC 6 (Fig 7A).For the dog-bone-shaped axial specimens, the difference was in E E , and DL 2 showed a significantly higher E E than DL 4 and DL 6 .For the  dog-bone-shaped oblique specimens, E E , ε u and σ u were different.The E E and σ u of DO 2 were significantly higher than those of DO 4 and DO 6 , whereas the value of ε u of DO 2 was significantly higher than that of DO 4 (Table 4).
For RC 6 and DC 6 , the ε u of RC 6 was lower than that of DC 6 , whereas the difference in σ u was not statistically significant.For RL 6 and DL 6 , the values of ε u and σ u of RL 6 were 1.96 times and 2.36 times lower than those of DL 6 , respectively (Table 4).
The results from different directions were not compared because they were punched from different locations.

Discussion
Although many aortic uniaxial tensile tests have been reported [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32], no attention has been paid to the influence of the geometry and size of the specimens on the test results; hence, there are no corresponding experimental protocols.In this study, we systematically investigated the influence of the shape and size of the specimens on the mechanical properties of porcine aortas by using custom-designed tissue cutters, clamps and molds and explored the optimal experimental conditions and parameters of material tests.

The use of custom-designed tissue cutters
In some published studies [37,42,43] with figures of aortic uniaxial tensile test specimens, the edges of the specimens are not sufficiently smooth or have notches, which will produce stress concentrations in these zones.The magnitude of these stress concentrations is usually expressed in terms of the stress-concentration factor K. K is independent of the material properties; rather, it depends only on the geometry and the type of discontinuity.The stress concentrations caused by sharp notches in specimens can lead to fracture at lower nominal stresses [44].In our study, custom-designed cutters could ensure smooth specimen edges and avoid potential stress concentrations.

The use of custom-designed clamps and molds
Another important factor for uniaxial tensile tests of soft tissue is the rapid clamping of the specimens and ensuring that the samples will not slip and fail in the clamping place.In some studies [14,15,21,22,24,29], the samples were clamped on grips using sandpaper.In other studies [26,27,31], the specimens were sandwiched between the clamps using sandpaper and glue.Our preliminary experiments showed that with the clamping method mentioned above, the strips easily slipped off when the clamping pressure was low or broke from the clamping point when the pressure was high.In the present study, custom-designed clamps were used to fix the strips.The gap between the clamps was 1.5 mm (the aorta was approximately 2 mm in thickness) (Fig 2C and 2D).The mechanical clamping of the clamps and the chemical adhesion of the glue ensured that the specimens did not slip and were not damaged by clamping; moreover, this process ensured that the samples did not fail at the clamps.Custom-designed molds with scales were utilized to fix the aspect ratio of the test zone and to quickly finish the preprocessing (Fig 2E and 2F).

The influence of the shapes of specimens
In previous studies, the shape of the strips was either rectangular or dog-bone-shaped, with a width ranging from 2 mm to 10 mm (Table 1).This is the reason why the shape and size of the specimens were chosen in this study.Rectangular specimens were not obliquely stamped because the lower diameter of the distal aorta made it difficult to ensure the corresponding aspect ratio.Sang Chao, et al. [45] studied the relationship between the specimen geometry and clamping conditions and the failure point, and their results showed that mid-sample failure occurred in 94% of dog-bone specimens, whereas this type of failure occurred in only 14% of the rectangular samples, which is similar to our findings.However, the effects on the mechanical parameters were not clarified in their study.According to our research, 88.9% of rectangular specimens failed close to the clamps, and the ultimate strain and stress of DL 6 were 1.96 and 2.35 times those of RL 6 , which had the same width and aspect ratio but different shapes.The reason for this phenomenon was mainly attributed to Saint-Venant's principle, which essentially states that the stress and strain produced at points in a body sufficiently removed from the region of load application will be the same as the stress and strain produced by any applied loads that have the same statically equivalent resultant and are applied to the body within the same region [46].The principle shows that in the uniaxial tensile test, the stress in the zone far away from the clamping positions is relatively uniform, and the stress near the clamping positions is uneven, so the specimens are prone to fail at the uneven stress location when the load is low.For the dog-bone-shaped specimens, the cross-sectional area near the clamping positions is relatively large, so the stress near the clamping positions with uneven stress is smaller than that in the test zone, making the stress in the test zone uniform and the failure point occur there.Because rectangular strips are easier to take and require lower aortic dimensions compared to dog-bone-shaped strips, rectangular strips are by fare the best option if video-extensometer is used in the central area with uniform stress for aortic uniaxial tensile test that measure physiological range and do not require stretching to rupture [38,47].However, when it comes to tension to rupture, rectangular samples are unsuitable for uniaxial tensile tests.

The influence of the sizes of specimens
Until now, there have been no comparative studies on the size effects of aortic walls or other biological specimens.This study shows that the sizes of the specimens have apparent influences on the aortic mechanical properties, regardless if the specimens are rectangular or dogbone-shaped.This effect is most obvious in the dog-bone-shaped circumferential specimens, and the ultimate strain and stress decrease with the increase in specimen width among the three groups (Fig 7A).The results should be attributed to the microstructure of the aortic wall.
The aorta is composed of the intima, media and adventitia.The intima consists of a single layer of endothelial cells, and it is mechanically negligible in healthy young individuals.The media is the middle layer of the artery and consists of a complex three-dimensional network of smooth muscle cells and elastin and collagen fibrils [48].M.K. O'Connell et al. [49] applied electron and confocal microscopy techniques to obtain 3D volumetric information of aortic medial microstructures and found that the media consisted of several concentric lamellar units bound together, each containing smooth muscle cells, whose long axis was radially tilted and angled more closely to the circumference and surrounded by collagen fibers embedded in the extracellular matrix.The adventitia consists mainly of fibroblasts and fibrocytes, histological ground substance and thick bundles of collagen fibrils forming fibrous tissue.The media and adventitia are composed of an isotropic matrix, and two families of collagen fibers helically wound along the arterial axis and symmetrically disposed with respect to the axis.The anisotropy in the mechanical response is induced by collagen fibers, so their response is orthotropic and can be considered a fiber-reinforced material [34,48].Collagen fibers of the two families are nearly symmetrically arranged with respect to the cylinder axis and are closer to the circumferential direction in the media but closer to the axial direction in the adventitia [50].Moreover, the elastic properties of the media and adventitia are different, and the media is much stiffer than adventitia [51][52][53].Therefore, for the intact aortic wall, the media should be the main passive load bearing, in which collagen fibers are close to the circumferential direction.Hence, the circumferential stretch of the artery wall is equivalent to the axial stretch of the fiber-reinforced material.For the fiber-reinforced material, the longitudinal strength is size dependent, and the strength of the material decreases with increasing diameter; however, the longitudinal modulus does not change appreciably [54].For brittle materials, it is generally believed that the probability of failure increases as the specimen size increases under the same stress conditions [55,56].The strength size effects of fiber-reinforced plastic (FRP) composite materials and structures have also been reported in other studies [57][58][59][60].The weakest-link theory was first proposed by Pierce [61], and Weibull [62] made great progress in this subject.This theory is usually used to analyze the strength size effects.The theory assumes the material is made up of smaller elements linked together and that failure of the material as a whole occurs when any of these elements or links fail.If the strength distribution of specimens is in accordance with Weibull theory, the strength values may be related to sample size.This can be expressed as follows: where σ and V are the strength and volume of the specimens, respectively, and m is the shape parameter.The equation expresses the relationship between stress and volume and thus quantifies the size effect.A logarithmic plot of stress versus volume gives a straight line relationship of slope -1/m.A detailed derivation is given in reference [59].

The influence of the shapes and sizes of specimens
It is speculated that the rectangular circumferential specimens failed under a smaller load due to Saint-Venant's principle, so they do not exhibit the same size effect as the dog-bone-shaped specimens.The ultimate stress of DC 6 was less than that of RC 6 , but there was no statistically significant difference between them.This could be explained by Weibull theory and Saint-Venant's principle.According to Weibull theory, the ultimate stress of RC 6 (aspect ratio 3:1) would be higher than that of DC 6 (aspect ratio 4:1).Moreover, rectangular specimens will easily fail at the uneven stress location near the clamping position under lower load due to Saint-Venant's principle.As a result, the RC 6 group with a small volume ruptured in advance.Therefore, the specimens should be processed into dog-bone shapes, and the aspect ratio of the test area must be reasonable and consistent for uniaxial tensile tests of aortas.
The optimal specimen choice for aortic uniaxial tensile testing performed until rupture The E E values of the 2-mm-wide dog-bone-shaped groups and the 6-mm-wide rectangular circumferential groups were higher than those of the corresponding other groups, whereas the values of E C did not show a significant difference.This may be because the width of the test zone of specimens was relatively small, making them more sensitive to load errors at the beginning of the uniaxial tensile test.The parameters (A and B) of the 2-mm-wide groups were different from those of the 4-mm-wide and 6-mm-wide groups, and the 6-mm-wide dog-boneshaped groups required a larger tissue size.Hence, the 4-mm-wide dog-bone-shaped group was more suitable for the aortic uniaxial tensile tests performed until rupture.

Limitations and future directions
The layer specificity of the aorta was not explored in our study for several reasons.(1) The aorta functions as a whole under the physiological states, and the mechanical properties of the whole layer are not a simple addition of three layers.(2) In the field of forensic injury biomechanics, layer-specific research on mechanical properties has little significance since these injuries are usually manifested as whole-layer rupture or dissection or other dangerous events.(3) This study is mainly focused on the effects of geometry and size on porcine aortic material properties and how to generate ideal material properties through our custom-designed instruments.In future research, we will use the optimized experimental method to test human aortas in a systematic group and explore how to process specimens and obtain accurate mechanical parameters for aortic tissues with relatively small lesions.

Conclusions
The study showed that sample geometry and size affect aortic uniaxial tensile tests.The rectangular specimen was not suitable for aortic uniaxial tensile testing performed until rupture according to Saint-Venant's principle.The size effect of the aorta conformed to Weibull theory.According to the results, dog-bone-shaped specimens with a width of 4 mm were the optimal choice for aortic uniaxial tensile testing performed until rupture.

Fig 2 .
Fig 2. Custom-designed cutters, clamps and molds used for specimen processing.(A) Customized cutter for rectangular sample with a 10 mm width.(B) Customized cutter for dog-bone-shaped samples with a 6 mm width.(C) Open state of the customized clamp.(D) Closed state of the customized clamp.(E) Processed RC 8 , for which the aspect ratio is 3:1.(F) Processed DC 6 , for which the aspect ratio of the test zone is 4:1.https://doi.org/10.1371/journal.pone.0244390.g002

Fig 3 .
Fig 3.The three presumed phases of the aortic elastic response and the relationship between the elastin fiber modulus, collagen fiber modulus and stress-strain curve.https://doi.org/10.1371/journal.pone.0244390.g003

Only 11 .
1% of the rectangular specimens failed in the middle part of the samples (Fig 4A), and 88.9% of them failed in the vicinity of the clamps.In contrast, mid-sample failure occurred in 85.2% of the dog-bone-shaped specimens (Fig 4B), whereas 14.8% of the dog-bone-shaped specimens failed in the vicinity of the clamps.

Fig 7 .
Fig 7. The ultimate values in the DC groups.(A) Comparison of ε u and σ u among the DC groups.(B) Logarithmic plot of the strength size effect of the DC groups.https://doi.org/10.1371/journal.pone.0244390.g007 Fig 7B shows a logarithmic plot of stress versus volume of the DC groups with the fitting equation ln(σ u ) = 2.566 − 0.306 ln(V).As shown in Fig 7B, the ultimate stress in the DC groups is basically consistent with Weibull theory.Hence, the size effect of the aorta can be explained by the Weibull theory.

Table 1 . Overview of uniaxial tensile test results on aorta full-thickness tests performed until failure.
Shanghai Key Laboratory of Crime Scene Evidence (No. 2019XCWZK03) and National Nature Science Foundation of China (No.81660309).Xuzhou Xinyou Industrial Control Technology Development Co., LTD.provided support in designing and manufacturing the customized tools in this study.The funders had no additional role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Xuzhou Xinyou Industrial Control Technology Development Co., LTD.provided support in designing and manufacturing the customized tools in this study.Additionally, the authors have two utility model patents to declare, the title and number of the first are "A clamping structure for the uniaxial tensile test of aorta" and 202020318949.7.The title and number of the second are "A tool for making standard test specimens for uniaxial tensile test of aorta" and 202020313014.X."There are no other patents, products in development or marketed products to declare.This does not alter our adherence to PLOS ONE policies on sharing data and materials.