In vitro and in vivo antifungal activity of Cuminum cyminum essential oil against Aspergillus aculeatus causing bunch rot of postharvest grapes

Bunch rot in grapes is an aggressive disease and needs to be controlled during the postharvest period. We investigate the antifungal potential of Zanthoxylum bungeanum Maxim., Zanthoxylum rhetsa, Cuminum cyminum, Coriandrum sativum, and Zingiber montanum (J. Koenig) Link ex A. Dietr. essential oils against Aspergillus aculeatus that cause bunch rot disease on postharvest grapes. C. cyminum essential oil exhibited stronger significantly inhibition percentage of 95.08% than other treatments in in vitro assay. Cumin aldehyde (33.94%) and α-terpinen-7-al (32.20%) were identified as major volatile compounds in C. cyminum oil. Antifungal potential of C. cyminum oil was then tested in conidia germination and in vitro tests compared to cumin aldehyde and α-terpinen-7-al. Their EC50 values against the conidial germination were also estimated. Significant reduction of conidia germination was also detected in C. cyminum essential oil and cumin aldehyde at a concentration of 1,000 and 100 μg/mL, respectively. EC50 values of the C. cyminum essential oil, cumin aldehyde, and α-terpinen-7-al were 67.28 μg/mL, 9.31 μg/mL, and 13.23 μg/mL, respectively. In vivo assay, the decrease of the disease severity (0.69%) and incidence (1.48%) percentage of A. aculeatus on grape berries treated at 1,000 μg/mL of C. cyminum essential oil was significantly greater than that obtained from other treatments after 10 days incubation. In addition, grape berries treated with C. cyminum essential oil decreased weight loss and retained fruit firmness. The changing of total soluble solids, total phenolic content, and antioxidant activity are also delayed in treated fruits. Therefore, essential oil of C. cyminum may be applied as a biological antifungal agent to control A. aculeatus in postharvest grapes without any negative effects on its quality.


Introduction
Grape (Vitis vinifera L.) is one of the world's most abundant fruit crops with various varieties [1]. FAO (2019) reported that grapes are produced at least 50  can be cultivated all over the world but mostly in China, which accounts for 16.8% of global production, followed by Italy and the United States at 10.9% and 9.6%, respectively [2]. Wide consumption of grapes and its products, such as wine, juice, jam and raisin showing its importance in worldwide economics. They are also generally known as an essential source of natural antioxidants, including flavonoids, phenolic acids, stilbenes, and lignans [3]. These compounds possess protective effects against many human diseases such as cancer and cardiovascular diseases [4]. In Thailand, grapes can be cultivated in many regions of the country. The Central region is the major production area of table grapes, while the Northeastern and Northern regions produce table and wine grapes. Beauty Seedless is the main grape variety cultivated in the Northern region due to its good growth, production, and quality, followed by Ruby Seedless and Early Muscat [5]. It is considered that grape production in Thailand has been successful. However, an increase in domestic consumption has made it necessary to import grapes from several countries at a value of one hundred million baht per year [6]. Grape quality depends on various factors such as cultivar type, climate, harvest and postharvest periods which affect their firmness, taste, flavor, and defect presence [7,8]. Quality degradation of grapes is mainly caused by infections from phytopathogens during cultivation and postharvest periods [9]. The major pathogen type that causes significant bunch rot disease in grapes during the postharvest period is the fungal pathogen Aspergillus aculeatus [10,11]. Infection by this pathogen contributes to a significant loss in quality and quantity during the transportation and storage process. Estimation of worldwide production loss is approximately 10 to 40% of total grape production in the world [12,13]. Sulfur dioxide is typically used to control bunch rot disease in grapes during cultivation and postharvest period [14,15]. Recently, its use in organic food has been made illegal due to environmental and health hazards, including residual toxicity, carcinogenicity, ecological pollution, and hormonal imbalance [15]. The development of safe and ecofriendly sustainable agriculture has been increased to control postharvest diseases with less impact on human, animal, and environment [16,17]. Therefore, readily accessible alternative treatments other than sulfur dioxide are needed for controlling bunch rot disease in fresh grapes during postharvest storage, transportation, and market periods.
Over the years, various biological agents have been applied extensively to control diseases from pests and phytopathogens, which is known as biocontrol [18]. Essential oils are considered as biocontrol agents inhibiting phytopathogen growth on fruit surfaces by in vitro and in vivo assay during the storage period [19]. The use of essential oils controlling postharvest phytopathogens is relatively inexpensive and may not require a sophisticated system. It can be performed by local farmers to extend the shelf lives of their agricultural products and is considered as an environmentally-and user-friendly technique. Although essential oils are advantageous as antimicrobial agent in controlling postharvest diseases, only a few studies have been reported on the control and inhibition of bunch rot disease in fresh grapes caused by A. aculeatus [10].
Cuminum cyminum L., a member of Apiaceae family, is widely known as an aromatic plant. Fruits of this plant is known as cumin seeds containing high amount of essential oil. Cumin seeds have been used therapeutic purposes in traditional medicine [20]. The essential oil of cumin seed is used as natural antioxidants, flavoring the foods and in the treatment of toothache, diarrhea, epilepsy, dyspepsia, and jaundice [20]. Cumin essential oil has been reported to possess antimicrobial activity against various human pathogens such as Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus haemolyticus, Propionibacterium acnes, Corynebacterium diphtheriae, and Candida albicans [20]. Moreover, this essential oil also exerted antifungal activity against various phytopathogens in postharvest fruits such as Aspergillus flavus, Penicillium italicum, Penicillium expansum, Penicillium commune, Rhizopus stolonifera, Rhizopus lyococcus, and Botrytis cinerea [21].
However, this study aimed to investigate inhibitory effect of essential oils produced by C. cyminum against the growth of A. aculeatus and its infection on fresh grape berries compared to those essential oils obtained from other plants; Zanthoxylum bungeanum Maxim., Zanthoxylum rhetsa, Cuminum cyminum, Coriandrum sativum, and Zingiber montanum (J. Koenig) Link ex A. Dietr. These essential oils from selected plants are known as Ayurvedic herbs, which is endemic to tropical Asia and have been used for centuries as herbal medicine to treat various symptoms such as astringent, asthma, bronchitis, diarrhea, and stomach aches. Previous reports have demonstrated that essential oils from these plants showing antimicrobial activities. Thus, the aim of the present study is to investigate the inhibitory effect of these essential oils against A. aculeatus in vitro and in vivo assay. The volatile compounds of plant species were also identified.

Extraction of essential oils
Each dried sample was subjected to hydrodistillation for 3 h according to procedures reported by Insawang et al. [22]. Anhydrous Na 2 SO 4 was added in the obtained essential oils to remove water prior placing pure essential oils in a sealed vial.

Isolation and identification of phytopathogen A. aculeatus
Isolation of fungal pathogen was performed using a modified method [23]. Infected berries of Beauty Seedless grapes (Vitis vinifera) were collected from DoiFang Vineyard Farm (195 1'59.8"N 99˚09'25.9"E), Fang district, Chiang Mai province, Thailand in July 2019. The collection site access has been approved by Ms Sawittree Kaewprasert, the owner of DoiFang Vineyard Farm. They were washed with sterile water before placing the fungal spores on Petri plates containing potato dextrose agar (PDA) mixed with chloramphenicol (30 μg/mL) to prevent bacterial growth. The plates were incubated at room temperature for seven days to complete fungal growth. The obtained fungus was further purified using the hyphal tips technique on PDA medium. It was identified based on micro-and macro-morphological characteristics using their cultures and spores according by Adesegun et al. [23]. In addition, the species level of isolated fungus was identified using molecular technique. The genomic DNA of isolated fungus was also extracted using a method of Tanapichatsakul et al. [24]. obtained sequences were compared to all sequences deposited in the National Center for Biotechnology Information database.

In vitro test of essential oils on the mycelial growth of A. aculeatus
The antagonistic activity of essential oils obtained from different plant species against A. aculeatus which caused bunch rot disease in Beauty Seedless grapes was evaluated using the contact phase method according to Aminifard & Mohammadi [25] and Pedrotti et al. [26]. with some modifications. The contact phase method was carried out using various concentrations of essential oils (200, 400, 600, 800, and 1,000 μg/mL) diluted in 5% v/v Tween 20. PDA (9.0 mL) was placed into 9 cm diameter Petri dish plates. A 100 μL sample of each essential oil solution was placed at the center of a Petri plate. After five minutes, an agar plug (5 mm) of pathogen A. aculeatus from seven days precultures was placed at the center of the PDA plate. All plates were sealed with Parafilm and further incubated at room temperature for seven days. Mycelia growth was determined after seven days. The mycelial diameter of A. aculeatus pathogen treated with essential oil in the PDA medium and the control group (no essential oil treatment) was measured daily with a digital Vernier caliper (MITUTOYO-ABS Digimatic Caliper CD-AX) in a mm unit. Plates containing 9.0 mL of PDA medium mixed with 5% v/v Tween 20 (100 μL) were used as control groups. The obtained results were reported as inhibition percentage of mycelial growth: [(mycelial diameter of the untreated-mycelial diameter of treated essential oil)/mycelial diameter of untreated essential oil) × 100]. There were five replicates for each treatment, and the experiment was repeated three times.

Viability test of the treated pathogen
To evaluate the viability of A. aculeatus after essential oils treatment in in vitro test, an agar plug (5 mm) of A. aculeatus pathogen was transferred to a fresh PDA plate and further cultured at room temperature for seven days. The mycelial growth was determined by measuring their mycelial diameter. This experiment was conducted with five replicates, and repeated three times.

Analysis of the volatile compounds of C. cyminum essential oil
The volatile compounds of C. cyminum essential oil was identified using gas chromatograph (HP6890, Agilent Technologies) interfaced to a mass-selective detector (HP model 5973, Agilent Technologies). The columns used was a HP-5MS (5% phenyl polymethylsiloxane) capillary column (30 m × 0.25 mm × 0.25 μm) (Agilent Technologies). Purified helium gas was used as the carrier gas. The temperature program was initially set at 60˚C and increased to 220˚C at a rate of 3˚C/min. Injector temperature was set at 250˚C while detector temperature was set at 280˚C, respectively. The ionization energy was 70 eV and molecular mass were scanned ranging from 30-300 AMU. The voltage of electron multiplier was programed at 1150 V. The temperature of ion source was set at 230˚C while quadrupole temperature was set at 250˚C. The volatile compounds were identified by comparing their retention indices relatively to C 8 -C 17 n-alkanes, with those obtained from Adams databases [27]. Moreover, their spectra were also compared to those found in the Wiley7N, NIST98 Mass Spectral Library. The quantitation of volatile compounds was performed using gas chromatograph (HP 6890, Agilent Technologies) equipped with a flame ionization detector. The volatile components were reported as the relative peak area percentage.

In vitro test of pure compounds on the mycelial growth of A. aculeatus
Antifungal activity of pure volatile compounds including cumin aldehyde and α-terpinen-7-al against A. aculeatus using the contact phase method described by Aminifard & Mohammadi [25] and Pedrotti et al. [26] with some modifications. These compounds were purchased from Hairui Chemical (China) and various concentrations of 20, 40, 60, 80, and 100 μg/mL were prepared by diluting in 5% v/v Tween 20. After that, 9.0 mL of PDA medium solution was placed into 9 cm diameter Petri dish plates. A 100 μL of each sample was placed at the center of a Petri plate. After five minutes, an agar plug (5 mm) of pathogen A. aculeatus from seven days precultures was placed at the center of the PDA plate. All plates were sealed with Parafilm and further incubated at room temperature for seven days. Mycelia growth was determined after seven days. The mycelial diameter of A. aculeatus pathogen treated with pure compounds in the PDA medium and the control group (no pure compound treatment) was measured daily with a digital Vernier caliper (MITUTOYO-ABS Digimatic Caliper CD-AX) in a mm unit. Plates containing 9.0 mL of PDA medium mixed with 5% v/v Tween 20 (100 μL) were used as control groups. The obtained results were reported as inhibition percentage of mycelial growth as explained previously. There were five replicates for each treatment, and the experiment was repeated three times.

Antifungal activity of C. cyminum essential oil and pure volatile compounds on conidia germination
C. cyminum essential oil showing strong inhibition percentage in in vitro experiments were selected for conidia germination experiments in accordance with the procedure described by Pedrotti et al. [26]. Pure volatile compounds including cumin aldehyde and α-terpinen-7-al were also tested to determine their effect against conidia germination of A. aculeatus. Conidia of 14-day old A. aculeatus were used in this experiment. The conidia were slowly poured in 5 mL of sterile water before scraping its surface using a sterile glass rod. The mycelia fragments extracted from the suspensions by filtering through three layers of cheesecloth. Conidial concentration of 1 × 10 6 conidia/mL was prepared using a hemocytometer under a microscope. A 50 μL conidia suspension was immersed in microtubes containing 500 μL of potato dextrose broth (PDB). Different concentrations of C. cyminum essential oil (200, 400, 600, 800, and 1,000 μg/mL) or pure volatile compounds (20,40,60,80, and 100 μg/mL) were prepared in 5% v/v Tween 20. PDB, with the addition of 5% v/v Tween 20 was used as the control treatment. All microtubes were further incubated at room temperature for 16 h before transferring samples to a hemocytometer tube. The number of conidia germination was counted under microscope at 10× magnification. EC 50 values were also calculated as the effective concentration that inhibited conidial germination by 50% in comparison to the control. This experiment was conducted with five replicates, in which 100 conidia were determined in each replicate. This experiment was repeated three times.

In vivo antifungal assay of C. cyminum essential oil and pure volatile compounds
The in vivo experiment was performed using the procedure described by Pedrotti et al. [16] with some modifications. All healthy Beauty Seedless grape berries collected from a greenhouse at DoiFang Vineyard Farm (19˚51'59.8"N 99˚09'25.9"E), Fang district, Chiang Mai province, Thailand located in March 2020. The collection site access has been approved by Ms Sawittree Kaewprasert, the owner of DoiFang Vineyard Farm. They were chosen based on their maturity, size, color without physical infections or injuries. The grape berries were sterilized by washing with distilled water for 2 min followed by 1% sodium hypochlorite for 60 s, and distilled water three times. Those berries were placed on a clean, pre-aseptic bench to airdry for 30 min before inoculation with the pathogens. One location on each berry was wounded using a sterile inoculating needle. Conidia of pathogen A. aculeatus was collected from its 14-day old colony in PDA at room temperature with a 12-h photoperiod. The suspension was prepared in sterile water to obtain a suspension concentration of 1 × 10 6 conidia/mL. The wound depth was approximately 2 mm. In the postharvest curative treatment, twenty healthy grape berries were inoculated simultaneously with 10 μL of a conidia suspension of A. aculeatus. They were then placed at the bottom of a 2 L plastic box that was covered with sterilized tissue paper. After 4 h, each grape berry was sprayed with C. cyminum essential oils at various concentrations of 200, 400, 600, 800, and 1,000 μg/mL or pure volatile compounds (cumin aldehyde and α-terpinen-7-al) solutions at various concentrations of 20, 40, 60, 80, and 100 μg/mL in 5%v/v Tween 20. Each box was closed with a fitted plastic lid and air-sealed using Parafilm. All boxes were separated in two groups and then kept at room temperature for 10 and 20 days, respectively. Different clusters of grape berries were prepared for each solution treatment: pathogen-uninoculated (negative control, NC), essential oil-treated (essential oil treatment, TE), cumin aldehyde-treated (cumin aldehyde TE), and α-terpinen-7-al-treated (α-terpinen-7-al TE) grape berries. Groups from NC treatments were treated with C. cyminum oil at a concentration of 1,000 μg/mL or pure compounds at a concentration of 100 μg/mL, while grape berries in the TE group were treated with C. cyminum oil and pure volatile compounds at various concentrations. Results from these clusters were compared to those obtained from the clusters without essential oil or pure compound treatments: pathogen-inoculated (positive control, PC) and pathogen-uninoculated (control, CC) grape berries. A digital Vernier caliper (MITUTOYO-ABS Digimatic Caliper CD-AX) was used to measure the mycelial diameter (mm) of A. aculeatus pathogen detected in each sample. Each treatment was conducted with five replicates. This experiment was repeated three times. In vivo antifungal effect was expressed in terms of disease incidence and severity percentage. Disease incidence was calculated as [(numbers of diseased fruit/total Number of fruit)×100]. Disease severity was calculated using the following formula: [(sum of symptomatic fruits and their corresponding score scale)/(total number of fruits × highest score scale)]×100. The severity scale was considered from 0 to 100% according to Pedrotti et al. [26].

Quality of grape berries obtained from all treatments
The quality of Beauty Seedless grape berries was evaluated after treatments with C. cyminum essential oil, cumin aldehyde, and α-terpinen-7-al for 10 and 20 days. In this experiment, weight loss percentage, fruit firmness, total soluble solids, and pH were measured following the study by Jahani et al. [28]. Total phenolic content and antioxidant activity of grape berries after treatment were determined following the study by Molyneux [29] and Yao et al. [30]. Each experiment was conducted with five replicates, and repeated three times.
Weight loss percentage. Weight loss of grape berries was determined as the percentage of weight loss with the following formula: (Initial weight-terminal weight)/(terminal weight) × 100.
Fruit firmness. Fruit firmness of samples was measured using a TA-XT Plus texture analyzer (Stable Micro Systems Ltd., UK).
Total soluble solids. Total soluble solids of the grape juices were determined at room temperature using a digital refractometer (RF 10, 0-32˚Brix, Extech Co., USA).
pH. The pH value of grape juices was measured at room temperature using a pH meter (Thermo Scientific Orion Star A111 Benchtop pH Meter, USA).
Total phenolic content and antioxidant activity. Grape berries from all treatments were homogenized and centrifuged at 13,000 rpm at room temperature for 10 min to extract their bioactive compounds. The obtained supernatants were used to be determined their phenolic content and antioxidant activity.
For the total phenolic content assay, extract (100 μL) was mixed with phosphate buffer (400 μL) and 10% v/v Folin-Ciocalteu phenol reagent (5 mL). After 1 min, 4 mL of 7.5% w/v sodium carbonate solution was added to the mixture and further kept in a dark place at room temperature for 1 h. Absorbance spectrum of the solution was determined using a UV/Vis spectrophotometer (Perkin-Elmer-Lamda 25) at 765 nm. Gallic acid was used as a standard.
Antioxidant activity of grape berries extracts was analyzed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Grape extract (1 mL) was added to 60 mM DPPH methanolic solution (1 mL). The solution was vigorously mixed and stored in a dark place at room temperature for 30 min. Absorbance of all solutions were determined using UV/Vis spectrophotometer (Perkin-Elmer-Lamda 25) at 517 nm. Trolox was used as a standard.

Data analysis
The differences in all experiments were analyzed using analysis of variance (ANOVA), using the SPSS 20.0 software. Duncan's post-hoc test was used to compare the mean values. A significance level at P < 0.05 was set to determine the significant differences. All data were reported as mean values with standard deviations.

In vitro antifungal activity of essential oils on the A. aculeatus mycelial growth
Z. bungeanum Maxim., C. sativum, Z. rhetsa, C. cyminum, C. sativum, and Z. montanum (J. Koenig) Link ex A. Dietr. presented essential oil yields of 0.28%, 0.48%, 0.57%, 0.89%, and 0.93%, respectively. All essential oils were screened in vitro antifungal activity against A. aculeatus on mycelial growth using contact phase assay. The effects of different essential oil concentrations on the A. aculeatus mycelial growth are listed in Table 1. The inhibition rate of essential oils on the A. aculeatus mycelial growth was most prominent at day 1, while the lowest was observed at day 7. A significant inhibition was observed until the day 7 compared to the control. High inhibition rate (>80%) was observed in C. cyminum essential oil from a concentration of 800 and 1,000 μg/mL at all days while other essential oils showed low inhibition rate (<50%) at most concentrations and number of days. Therefore, C. cyminum essential oils had significantly highest inhibition rate and were chosen for further tests.

Viability of the treated pathogen
After the treatment of A. aculeatus with the essential oils C. cyminum, pathogen A. aculeatus was able to grow and produce its mycelia slowly again on the PDA plates. The diameters of A. aculeatus pathogen after treating and without treating with C. cyminum essential oil on medium agar plates are shown in Fig 1. The growth of A. aculeatus after treating with C. cyminum essential oil was significantly slower than those obtained from untreated pates. After 14 days, diameter of A. aculeatus pathogen after treating and without treating with C. cyminum was 2.51 cm and 8.63 cm, respectively.

In vitro test of pure compound on the mycelial growth of A. aculeatus
All concentrations of pure major volatile compounds of C. cyminum oil (cumin aldehyde and α-terpinen-7-al) were tested to determine their antifungal activity against A. aculeatus using contact phase assay that showed in Table 3. The reduction on mycelial growth of A. aculeatus was most prominent at day 1 and continuously reduced at day 7. High inhibition rate (>80%) was recorded significantly in cumin aldehyde at concentration 60, 80, and 100 μg/mL, respectively, at day 1. Meanwhile, at all concentrations and number of days of α-terpinen-7-al showed low inhibition rate (<70%) at most concentrations and number of days. Hence, cumin aldehyde had significant inhibition rate on the mycelial growth of A. aculeatus.

Antifungal activity of C. cyminum essential oil and pure volatile compounds on conidia germination
The inhibitory effect of C. cyminum essential oil and pure volatile compounds from different concentrations on conidia germination of A. aculeatus is depicted in Fig 2. The conidia germination of A. aculeatus was significantly lower in the highest concentration. At all

In vivo antifungal assay of C. cyminum essential oil, cumin aldehyde, and α-terpinen-7-al
Grape berries after 10-and 20-day incubation from various treatments are shown in Figs 3 and 4, respectively. No physical damage on the grape berries was detected in the CC and NC treatments among all treatments. Less physical damage was observed on grape berries inoculated with A. aculeatus and treated with C. cyminum oil (�800 μg/mL) and cumin aldehyde (100 μg/mL) after 10-day incubation. Moderate physical damage was observed on grape berries inoculated with A. aculeatus and treated with C. cyminum oils at concentrations of �600 μg/mL, cumin aldehyde at concentrations of �80 μg/mL, and α-terpinen-7-al at all concentrations after 10-day incubation. However, high physical damage was observed on grape berries inoculated with A. aculeatus and treated with all concentrations after 20-day incubation. Incidence and severity percentage of A. aculeatus in grape berries treated with C. cyminum oil, cumin aldehyde, and α-terpinen-7-al from various concentrations after 10-and 20-day incubation are shown in Fig 5. Significant reduction of the incidence and severity percentage was observed in all treatments. After 10-day incubation, C. cyminum oil (1,000 μg/mL) and cumin aldehyde (100 μg/mL) treatments showed the highest reduction among these concentrations with 1.48% incidence and 0.69% severity for C. cyminum oil and 2.04% incidence and 1.11% severity for cumin aldehyde while no significant reduction of the incidence and severity percentage was observed in α-terpinen-7-al treatment at concentration of 60 μg/mL. After 20-day incubation, the highest reduction of incidence and severity percentage was also detected from the treatment with C. cyminum oil followed by cumin aldehyde, and α-terpinen-7-al at the highest concentration with % incidence of 3.70, 5.56, and 25.93, and % severity of 2.59, 3.33, and 18.06, respectively.

Quality of grape berries after treatments
The effects of C. cyminum oil, cumin aldehyde, and α-terpinen-7-al essential oils on the quality of grape berries after 10-and 20-day incubation in terms of weight loss percentage, firmness, total soluble solids, pH, total phenolic content, and antioxidant activity are depicted in Tables  4 and 5, respectively. After ten days of treatments, no significant difference was observed for pH of the grape berries from all treatments (3.24-3.43) while other qualities including % weight loss, firmness, total phenolic content, antioxidant activity and total soluble solids were significance among these treatments. Weight loss percentage, firmness, total phenolic content, antioxidant activity and total soluble solids among these treatments were ranged from 1.

Discussion
Essential oils from five plant species were selected for this study due to their broad-spectrum antimicrobial activity. According to previous studies, these essential oils were reported to produce volatile compounds showing antimicrobial activity against Aspergillus niger and Candida species [31,32]. Moreover, they have been evaluated to inhibit some phytopathogens such as Penicillium lilacinum, A. niger, A. flavus and Botrytis cinerea [33][34][35][36]. Therefore, essential oils extracted from these plant species may be capable of producing volatile compounds that suppress the bunch rot disease caused by A. aculeatus in postharvest grapes. Among these essential oils, C. cyminum essential oil showed high antifungal activity inhibiting the A. aculeatus mycelial growth and its infections on postharvest grapes. Based on in vitro assay, the volatile compounds produced by C. cyminum essential oil only showed high antifungal activity and their effectiveness gradually decreased over time (Table 1). This result was good agreement to the reports [36,37] in which essential oils of C. cyminum oil showed potent inhibitory activity against A. fumigatus. In this study, C. cyminum essential oil was effective in controlling A. aculeatus at all concentrations, while low concentration of C. sativum, Z. montanum (J. Koenig) Link ex A. Dietr., Z. bungeanum Maxim., and Z. rhetsa essential oils (200 and 400 μg/mL) were sufficient to control A. aculeatus.
Stronger inhibitory effect on mycelial growth and conidia germination of A. aculeatus were observed in C. cyminum oil than the pure volatile compounds treatment. This result was similar to the study from Edris & Farrag [38] reporting high antifungal activity of sweet basil essential oil compared to those obtained by pure major compounds. Moreover, Cavaleiro et al. [39] also reported that Juniperus essential oil showed greater antimicrobial activity that those found in their major compounds such as α-pinene, β-phellandrene, and myrcene.
The in vivo study, after 10 days of storage at room temperature, showed that treatments by C. cyminum essential oil at 1,000 μg/mL effectively reduced incidence and severity percentage  [40,41]. In addition, the mycelial growth of A. aculeatus was inhibited by Cinnamomum zeylanicum, Ocimum gratissimum, Syzygium aromaticum and Eugenia caryophyllata essential oils [42][43][44]. In vivo effectivity of C. cyminum essential oil was probably due to the antifungal activity of their volatile compounds. Generally, complexity of the essential oil composition has been reported to influence its antimicrobial property [45]. Antifungal activity of the C. cyminum essential oil may be attributed to cumin aldehyde and α-terpinen-7-al [46,47]. According to previous studies, cumin aldehyde has

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functional and physical decay of cell tissue of fungi, thereby causing cell membrane leakage [52]. Moreover, essential oils also have antimicrobial properties due to their disruption properties on the mycelial growth by reduction of microbial cellular metabolism [53,54]. Uribe et al. [55] and Sikkema et al. [56] reported that their antifungal action of several monoterpenes was performed at the membrane level or embedded enzymes. Changing of the fatty acid

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composition of the cell membrane, alteration of permeability, and inhibition of respiration were suggested as possible mechanisms for antifungal propertied of monoterpenes [57,58].
Marei & Abdelgaleil [59] also reported that the monoterpenes showed antifungal activity through the inhibition of pectin methyl esterase and cellulase enzymes. They were considered as the potent inhibitors of pectin methyl esterase modifying the degree of methylesterification of pectin, major component of fungal cell walls. Changing of pectin structure were resulted in cellular adhesion, plasticity, pH, and ionic contents of the cell wall and influenced fungi development, membrane integrity and permeability. Evaluation of post-treatment grape berries quality indicates that C. cyminum essential oil having concentration of at least 400 μg/mL could delay the ripening process in grape berries by inhibiting A. aculeatus infection without affecting certain grape berries qualities after 10 days. The quality of fresh fruit depends on several factors including weight loss, firmness, total soluble solids, total phenolic content, and antioxidant activity. Serrano et al. [52] reported the efficacy of thymol and eugenol essential oils exposure on cherries and grapes without effects. Fennel and thyme oil were reported to reduce loss of grape weight [60] while cinnamon and eucalyptus essential oils do not affect weight loss in strawberries and tomato fruits [61]. In similar reports, Ocimum basilicum essential oil coated on Embul banana peel to control crown rot and anthracnose did not have any significant effect on the total soluble solid [62]. Ulukanli & Oz [63] reported that there are no significant differences in pH value in strawberries treated with myrtle essential oil. In addition, Wang et al. [64] found that thymol, eugenol, and menthol from essential oils enhanced phenolic and antioxidant activity in strawberries.

Conclusion
This investigation suggests C. cyminum essential oil as an alternative biological fungicide to control bunch rot diseases caused by A. aculeatus on postharvest grape berries. Considering all evaluated parameters on grape quality within the scope of this study, C. cyminum essential oil treatment was shown to delay grape ripening. Total phenolic content and antioxidant activity assay also showed that postharvest grape berries treated with C. cyminum essential oil could be stored while maintaining their quality for up to ten days. However, further studies are required before applying C. cyminum essential oil treatments as a biocontrol agent to increase the postharvest period of grapes.