Investigating the effects of incremental conditioning and supplemental dietary tryptophan on the voluntary activity and behaviour of mid-distance training sled dogs

Serotonin is a neurotransmitter synthesized by the amino acid tryptophan, that has the potential to impact the behaviour and activity of dogs. The objective of this study was to assess the effects of supplemental tryptophan and a 12-week incremental training regimen on the voluntary activity and behaviour of client-owned Siberian Huskies. Sixteen dogs were blocked for age, BW and sex and then randomly allocated to either the control or treatment group. Both groups were fed the same dry extruded diet; however, the treatment group were supplemented with tryptophan to achieve a tryptophan: large neutral amino acid ratio of 0.075:1. Once a week, a 5-minute video recording was taken immediately pre- and post- exercise to evaluate dogs’ behaviours. Activity monitors were used to record voluntary activity on both training and rest days. Linear regression analysis was used to assess the relationship between training week and time spent performing each behaviour. Additionally, a repeated measure mixed model was used to test differences between diet groups and training week for both behavioural and activity count data. The time spent performing agonistic behaviours prior to exercise was negatively associated with week for treatment dogs (β = -0.32, 95% CI [-0.55, -0.10], P < 0.05) and no change was observed for control dogs (β = -0.13, 95% CI [-0.41, 0.15], P > 0.10). Treatment did not have any effect on activity levels (P > 0.10). For all dogs, locomotive behaviours decreased prior to exercise as weeks progressed (P < 0.05), while run day voluntary activity depended on the distance run that day (P < 0.05). These data suggest that sled dogs experience an exercise-induced reduction in voluntary locomotion in response to both single bouts and repetitive bouts of exercise. Additionally, tryptophan supplementation may decrease agonistic behaviours, without having any effect on voluntary activity.

Introduction Sled dogs are endurance athletes that perform high levels of repetitive aerobic and resistance exercise. The success of sled dogs depends on multiple factors, including aerobic capacity and physical fitness as well as workability and trainability. While physical fitness is of primary importance, in order to achieve and maintain the level of fitness required to compete at a high level, it is critical to ensure that sled dogs continue to be motivated to exercise throughout their training and racing seasons. However, repetitive training programs can induce oxidative stress and muscle damage in working dogs [1,2], which can result in muscle fatigue and soreness [3]. While researchers have examined the physiological effects of exercise and anticipation of exercise in sled dogs [4], more focus is needed on understanding how exercise can impact behaviour in both the short and long term. Exercise-induced muscle fatigue, as well as overtraining, can lead to decreased mood and lack of motivation to exercise in humans [5]. In canines, these symptoms may manifest as behavioural changes, such as a reduction in locomotive behaviours prior to a bout of exercise, or a generalized decrease in voluntary daily activity. For working dogs, voluntary activity consists of physical activity performed outside of scheduled training or racing bouts. However, there is a dearth of literature that defines how commonly implemented training regimens and repetitive bouts of exercise may impact the behaviour of performance dogs.
Exercise capacity and motivation are not the only important factors to consider when investigating the performance of sled dogs. Sled dogs work in teams of 2 to 18 and typically interact with one or more handlers throughout their lives. Therefore, another key component to a successful sled dog is the ability to work in close proximity with other dogs and humans. Undesirable behavioural states commonly reported in working dogs include fear and anxiety [6,7]. As well, both inter-dog and human-directed aggression can result in a poor team environment and may lead to pain and injury [7]. For sled dogs, inter-dog aggression can present itself as contact or non-contact social conflict, which are forms of agonistic behaviours. Previous research has found that dogs who display aggressive behaviours have lower serum and central serotonin concentrations than non-aggressive dogs [8,9].
Serotonin, a neurotransmitter associated with regulation of mood, is synthesized in the brain from the amino acid tryptophan (Trp) [10]. Increased serotonin can enhance stress resistance [11] and reduce the prevalence of undesirable emotional states and behaviours, such as anxiety [12], fear [13] and agonistic behaviours [14] in numerous monogastric species, including dogs [15]. Serotonergic activity is also linked to alterations in general voluntary activity and locomotion [16,17]. Tryptophan has sedative effects in humans [18] and increasing central serotonin during exercise has been proposed to be associated with feelings of lethargy and a lack of motivation [19]. However, other research has presented conflicting results, with some reports indicating that increased levels of dietary Trp decreases fatigue perception in humans [20,21], and has no effect on hyperactivity in client-owned dogs [15]. Increasing central serotonin concentrations may reduce various locomotory behaviours and activity; however, no previous research has looked at the effects of Trp-supplementation on the locomotive behaviours or voluntary activity levels in actively-training sled dogs.
Supplementing Trp in canine diets has been previously investigated as a means of increasing the production of serotonin [22,15]. However, Trp competes with the large neutral amino acids (LNAA) for transport across the blood-brain barrier. Most protein-containing ingredients have lower concentrations of Trp relative to other amino acids, thus resulting in a reduced Trp:LNAA ratio [10]. Therefore, diet formulation, while still meeting the requirements for Trp, may lead to an imbalance of Trp and LNAA. Thus, the ratio of Trp:LNAA should be considered when formulating diets to ensure adequate transport of Trp to the brain for serotonin synthesis. The suggested Trp:LNAA ratio is 0.061:1 [23], although the optimal ratio may be greater [24] suggesting that current sporting dog diets may not adequately supply Trp to support serotonin synthesis. Therefore, increasing dietary Trp and the ratio of Trp:LNAA may result in an increase in central serotonin production and a reduction in agonistic or other undesirable behaviours in actively training sled dogs.
The objective of this study was to investigate the effects of 12 weeks of incremental conditioning and supplemental dietary Trp on the voluntary activity and pre and post-exercise gangline behaviours of mid-distance training sled dogs. We hypothesized that dietary supplementation of Trp would decrease the time spent performing various locomotive and agonistic behaviours, pre-and post-exercise, as well as decrease daily voluntary activity, due to an increase in serotonergic activity. We also hypothesized that as exercise intensity and duration increase, the voluntary activity and locomotive behaviours performed would decrease due to exercise-induced physical fatigue.

Animals, training regimen and diet
All procedures and facilities were approved by the Animal Care Committee at the University of Guelph (AUP #4008). Sixteen client-owned domestic Siberian Huskies (9 female: 4 intact, 5 spayed; 7 males: 2 intact, 5 neutered), with an average age of 4.8 ± 2.5 years and body weight (BW) of 24.3 ± 4.3kg, were housed, fed and trained at an off-site facility (RaJenn Siberian Huskies, Ayr, Ontario, Canada). Verbal and written consent was given by the owners for the use of their animals. A training regimen was proposed where dogs ran in a standard 16-dog gangline formation four times a week (Mon-Thurs) and distance increased incrementally over a 12-week period. Dogs ran in the same position on the gangline throughout the study. Dogs were anticipated to run 8km during week 0 and reach 86km during week 11; however, due to inclement weather, the training regimen was adjusted (Table 1; refer to Templeman et al. [25] for full proposed and adjusted training regimen). Daily temperature was recorded (Table 1). Total distance travelled over the training period was reduced from~1900km to~1230km. When training, dogs pulled an all-terrain vehicle carrying one passenger while maintaining an average speed of approximately 15km per hour throughout the study period. Training began consistently at 08:30 h. When not running, dogs were group housed in free-run outdoor kennels ranging from 3.5 to 80 square meters, containing anywhere from 2-10 dogs each. Two dogs were removed from the trial (one CON dog on week 7 and one TRT dog on week 9) due to exercise-related injuries. All data collected up until their respective points of removal are included in this report. Dogs were blocked for age, gender and BW and then randomly assigned into one of two groups (n = 8 per group): the control group (CON), fed a dry extruded diet (Champion Petfoods LT., Morinville, AB; refer to Templeman et al. [25] for full diet formulation) formulated to meet or exceed all AAFCO (2016) nutrient recommendations, or the treatment group (TRT), fed the control diet top-dressed with dietary Trp so as to reach a Trp:LNAA ratio of 0.075:1. Tryptophan solution was prepared by dissolving 10g of crystalline Trp (ADM Animal Nutrition, Woodstock, ON) per L of deionized water heated to 30˚C. The solution was brought to room temperature (22˚C) and stored at 4˚C until use. Each diet was stirred for 10 minutes after the addition of Trp solution to equally coat all kibble and ensure homogenous incorporation. Dogs were individually fed once a day at approximately 16:00 h to maintain BW, with individual BW recorded weekly. Dogs were provided ad libitum access to clean water.

Behavioral evaluation
Using a digital camera (Sony HDR-CX110 HD Handycam, Sony Corp., Tokyo, Japan), video recordings were taken on one day (either Day 1 or Day 2) of each week to evaluate changes in the dogs pre-and post-exercise behaviours, where the distances they ran depended on week (Table 1). Once all dogs were put into their harnesses and individually placed in their respective positions on the 16-dog gangline, they were recorded for 5 continuous minutes immediately prior to exercise. Upon return from the training bout, 5 minutes of continuous video was again recorded while the dogs remained on the gangline. Dogs had been previously acclimatized to remain on the gangline post-exercise. Immediately upon cessation of the video, dogs were removed from the harness, and returned to their respective pens. Ten out of the 16 dogs (5 CON dogs and 5 TRT dogs) were chosen to be recorded, based on gangline position and visibility, to identify the occurrence of the following behaviours: jumping, lunging, changing posture, chewing on the gangline, sitting, lying, standing, digging, and changing posture ( Table 2). All behavioural analysis was completed by a single individual who was blind to Table 2. Description of behavioural parameters analyzed during 5 minutes of video taken immediately pre-and post exercise for dogs fed either a treatment diet containing supplemental Trp compared to dogs fed control diet.

Behaviour Description
Sitting Positioned with rear end and two front paws in contact with the ground

Activity monitoring
Three-dimensional accelerometers (Fitbarks, Fitbark Inc., Kansas City, MO) were attached to the collars of each dog to record activity on weeks 0, 6, and 11 (Table 1). For each of those weeks, activity was evaluated continuously for 24 hours during a rest day (no training) and again on an active day (training). Data is expressed as an activity count, which represents physical activity and is generated by company algorithm. Similar to a step count generated by a human activity monitor, the voluntary activity counts described herein represent physical activity and a larger voluntary activity count indicates the dog is more active. While activity was being recorded, any periods of human interference, such as feeding, owner interaction, or training, were noted and subsequently removed from the activity count data. This ultimately left 3 hours of uninterrupted data that represented the voluntary activity performed by the dogs in their kennels, which was used for further analysis. For rest days, 3 consecutive hours of data were used from 11:00 h to 14:00h, while for active days, total activity counts were combined from 1-h pre-run (7:00 h to 8:00 h), 1-h post-run (dependent on run finishing time) and 1-h post-feeding (18:15h to 19:15h). Activity data from 4 CON dogs was removed from the week 1 rest day due to unanticipated owner interaction.

Statistical analysis
The time spent performing a behaviour was converted into percentage of time [(duration of behaviour/duration of recording) x 100]. The average length of a bout of agonistic behaviour was also calculated [sum of duration of bouts/number of bouts]. The relationship between training week and the percentage of time performing a behaviour was analyzed using PROC REG of SAS (v.9.4; SAS Institute Inc., Cary, NC). If both TRT and CON groups had similar significant regression slopes for a particular behaviour, data were pooled and reanalyzed using PROC REG of SAS (v.9.4; SAS Institute Inc., Cary, NC). Behavioural data were also analyzed using PROC GLIMMIX of SAS (v.9.4; SAS Institute Inc., Cary, NC), with dog as a random effect and week and treatment as fixed effects. Week � treatment interaction effects were analyzed but removed if insignificant. Week was additionally treated as a repeated measure. Means were separated using Fisher's LSD. Results are reported as least square means (LSM) ± standard error (SE). For all models, residuals were tested for homogeneity and normality by using the Shapiro-Wilk test and plots. PROC CORR of SAS (v.9.4; SAS Institute Inc., Cary, NC) was used to assess the relationship between daily temperature (˚C), behaviour and week.
Significance was declared at P � 0.05, and trends at 0.05 < P � 0.10. Activity counts during rest days and training days were analyzed separately but using the same statistical method. Data were analyzed using the PROC GLIMMIX of SAS (v.9.4; SAS Institute Inc., Cary, NC) with dog as a random effect and week and treatment as fixed effects.
Week was treated as a repeated measure. Week � treatment interaction effects were also analyzed. Means were separated using the Tukey adjustment. Results are reported as LSM ± SE. Additionally, PROC REG of SAS (v.9.4; SAS Institute Inc., Cary, NC) was used to evaluate the relationship between distance of exercise bout and run day activity counts. PROC CORR of SAS (v.9.4; SAS Institute Inc., Cary, NC) was used to assess the relationship between daily temperature (˚C) and activity. For all models, residuals were tested for homogeneity and normality by using the Shapiro-Wilk test and plots. Significance was declared at P � 0.05, and trends at 0.05 < P � 0.10.

Behaviour
Pre-exercise gangline behaviour. There was a negative association between the duration of a bout of agonistic behaviours and week of training for dogs receiving Trp-supplementation (β = -0.32, 95% CI [-0.55, -0.09], R 2 = 0.10, P = 0.007; Fig 1B); however, no association was observed for dogs receiving the control diet (β = -0.13, 95% CI [-0.41, 0.15], R 2 = 0.01, P > 0.10; Fig 1A). For all other behaviours, similar regression slopes were found between CON and TRT dogs; therefore, data were pooled to assess the effects of exercise. When the data were pooled, there was a negative association between week of training and time spent lunging and changing posture, and a positive association between week of training and time spent lying down (P < 0.05; Table 3). No associations were found between training week and time spent chewing on the line, digging, sitting or standing (P > 0.10; Table 3).
There was no effect of dietary treatment on the overall time spent performing any pre-run behaviour evaluated (P > 0.10; S1 Table), therefore data from all dogs were pooled to examine week by week differences in behaviour. The time spent lunging was greater during weeks 1 and 2 than the latter weeks (weeks 7, 9 and 11) (P = 0.025; Table 4). Time spent changing posture was greater during weeks 0 to 5 than weeks 9 to 11 (P < 0.001; Table 4). Time spent lying down was greater during the latter weeks, specifically greater in week 9 than any other week, and greater during weeks 7 and 11 than weeks 0 to 2 and 4 to 6 (P < 0.001; Table 4). Week tended to have an effect on time spent sitting (P = 0.065; Table 4) but had no effect on time spent performing agonistic behaviours, chewing on the gangline, digging, jumping or standing (P > 0.10; Table 4).
Post-exercise gangline behaviour. The only behaviours observed post-exercise were sitting, standing or lying. Similar regression slopes were found between CON and TRT dogs for the time spent performing any behaviour and week; therefore, data from all dogs were pooled. There was a positive association between week of training and time spent lying down and a negative association was found between week of training and time spent standing (P < 0.05; Table 3). No association was found between week and time spent sitting (P > 0.10; Table 3).
There was no effect of dietary treatment on the overall time spent performing any behaviour evaluated (P > 0.10; S2 Table), therefore data from all dogs were pooled to examine week by week differences in behaviour. The time spent standing was greater during the earlier weeks (weeks 0 to 7) then weeks 8 and 11 (P < 0.05; Table 5). In contrast, the time spent lying down was greater during the latter weeks (weeks 8 and 11) than weeks 0 to 7 (P < 0.05; Table 5).
Week had no effect on time spent sitting (P > 0.10; Table 5).

Voluntary activity
Treatment had no effect on activity counts during rest days or active days throughout the 12-week conditioning period (P > 0.10; Table 6). When data from all dogs were pooled, total activity count on rest days (no regimented exercise) decreased from week 0 to week 6 and from week 6 to week 11 (P < 0.05; Fig 2A). Total activity counts on run days (regimented exercise) decreased between week 0 and 6 (P < 0.05); however, run day activity counts on week 11 did not differ from either week 0 or 6 (P > 0.05; Fig 2B). Additionally, total activity counts on active days was negatively associated with the distance run that day (β = -14.59, 95% CI [-22.03, -7.15]; P < 0.05). Daily environmental temperature was positively correlated with off day activity (r = 0.62, P <0.05) and run day activity (r = 0.31, P <0.05).

Discussion
To the authors' knowledge, this is the first study to evaluate the effects of dietary Trp and an incremental training regimen on the pre-and post-exercise behaviour and voluntary activity of dogs. Dietary Trp may influence behaviours related to anxiety [12], stress [11] and fear [13]. In the present study, dogs receiving Trp supplementation experienced a reduction in agonistic behaviours prior to exercise throughout a 12-week period. However, Trp-supplementation did not affect any other observed behaviour, nor did it affect the voluntary activity of dogs during training or rest days. Although previous research has suggested that dietary Trp can have a sedative effect resulting in a decrease in locomotion in humans [18] and mice [17], we found no  effect in actively training dogs. In agreement with the present work, research by DeNapoli et al. [15] found that dietary Trp supplementation did not reduce hyperactivity in dogs, which the authors characterized by criteria including excessive pacing, chewing of objects and the inability to remain in a sit position. These behaviours are comparable to the pre-exercise behaviours observed in the current study, which were also unaffected by dietary Trp supplementation. Additionally, Bosch et al. [22] found no differences in the percentage of time spent changing posture, walking or lying down in an open-field test following 8 weeks of Trp supplementation in client-owned dogs. Combined, these results suggest that Trp supplementation does not influence activity levels and associated locomotive behaviours in domestic dogs.

Tryptophan effect on aggression-related behaviours
Although Trp did not appear to affect overall locomotion in sled dogs, there was a significant effect of the dietary treatment on agonistic behaviours performed throughout the study period. A decrease in aggression following dietary Trp supplementation has been previously reported in pigs [26] chickens [27], vervet monkeys [28] and rats [14]. As well, dogs previously diagnosed with aggression that were being fed a high protein diet supplemented with additional Trp to reach a Trp:LNAA of 0.07:1 showed reduced aggressive behaviour [15]. In the present study, no preliminary evaluations were performed to identify any potential behavioural predispositions; however, Trp-supplementation still decreased the time spent performing agonistic behaviours prior to exercise over the 12-week period. The Trp:LNAA ratio of 0.075:1 in the treatment diet resulted in increased serum Trp concentration in the treatment dogs compared to the control dogs [25], which favors the conditions for higher transport of Trp across the blood-brain barrier. Theoretically, this suggests that more Trp will be converted to serotonin in the brain which is thought to be involved in the regulation of aggressive behaviour, likely through the enhancement of impulse control [29]. Male rhesus macaques with low levels of cerebrospinal fluid 5-hydroxyindoleacetic acid (CSF 5-HIAA), which is the primary metabolite of serotonin, were found to be more likely to show aggressive behaviours as well as experience a loss of impulse control characterized by greater risk-taking behaviours [30]. Dogs who demonstrated impulse aggression, identified by biting without warning, also had lower concentrations of CSF 5-HIAA than non-aggressive dogs [31]. Through the action of central serotonin, treatment dogs in our study may have experienced improved impulse control causing the slight reduction in agonistic behaviours prior to exercise. While the recommended Trp:LNAA ratio derived from minimal AA requirements suggested by the National Research Council is 0.061:1 [23], the ratio needed to influence a behavioural response may be higher. Various inclusion levels of Trp in canine diets leading to Trp:LNAA ratios of 0.0274:1, 0.0403:1, 0.0448:1, and 0.0581:1, all had no impact on aggressive behaviours in response to a familiar or unfamiliar human in mixed-breed hounds [32]. However, a Trp:LNAA ratio of 0.075:1 used in the present study, and 0.07:1 used by DeNapoli et al. [15] both caused reductions in agonistic behaviours. This suggests that diets aimed to reduce agonistic behaviours should be formulated to include a Trp:LNAA ratio of over 0.07:1 in order to elicit the desired behavioural change.
The results of this study further contribute to the existing literature that dietary Trp can influence behaviours related to aggression, with no apparent sedative effects on locomotive activity or behaviour. In the present study, the average level of agonistic behaviour was minimal during any given week (< 5%). Since no behavioural evaluations were performed prior to the beginning of the study, the dogs were not able to be equally distributed into treatment and control groups based on any behavioural parameter. Future research should consider incorporating a baseline evaluation of behaviour to ensure adequate inclusion of dogs that are known to exhibit agonistic behaviours, which may lead to more robust differences between diet groups.

Effect of single bout and repetitive exercise on behaviour and activity
In addition to the effects of dietary treatment on agonistic behaviours, additional findings from this study revealed that a 12-week conditioning period influenced the locomotive behaviours and voluntary activity of sled dogs, regardless of dietary treatment. Endurance exercise causes physiological changes, and recovery from exercise involves restoration of endogenous energy stores and a return to a normal heart rate, respiratory rate, and internal temperature [33]. In addition to the inevitable physiological impact, intense aerobic exercise in human athletes can lead to an increased risk of oxidative stress and the subsequent skeletal muscle damage is associated with soreness, reduced range of motion, and muscle fatigue [3,34]. These physiological effects and the extent of recovery depend on the duration and frequency of exercise [35,36]. In the present study, the amount of voluntary activity performed by the dogs in their free-run kennels during days of active training depended upon the distance run that day, which likely represents a decrease in available metabolic energy due to exercise. During the post-exercise period, dogs are likely resting, in part, to restore endogenous energy and return to homeostasis. Furthermore, as indicators of oxidative stress and muscle damage are linked to the intensity of aerobic exercise [37,38], it is expected that voluntary activity performed by sled dogs would be dependent on the duration of an exercise bout. This suggests that voluntary activity surrounding exercise may be useful as an additional indicator of intensity of a bout of exercise in sled dogs. While voluntary activity was found to be related to the duration of a single bout of exercise, it is also evident that behaviour and locomotion may be additionally affected by repetitive exercise. For all dogs, there was an overall decrease in voluntary activity during rest days and a reduction in locomotive behaviours pre-exercise over the 12-week study period. Although no markers of metabolic stress were measured in the present study, previous research has shown that repetitive exercise in sled dogs is associated with increased creatine kinase (CK) concentrations, which is an indirect marker of skeletal muscle damage [39]. Sled dogs who ran 58-km on each of three consecutive days had a significant increase in serum CK following the first exercise bout, with a further significant increase after the third exercise bout [1]. Although the distances run in the present study did not exceed 53km a day, it is possible that similar muscle fatigue was experienced, which caused the documented reduction in voluntary activity and locomotive behaviours. Taken together, this suggests that while short-term voluntary activity is related to the distance of an exercise bout on a specific day, activity and locomotion can also be affected in the long term by the repetitiveness of a training regimen. Sled dogs are simultaneously experiencing both an acute and chronic response to exercise, which should be considered when designing training regimens to optimize exercise programs. It is important to note that ambient temperature may also affect behaviour. Interestingly, in the present study, sled dogs were less active and exhibited fewer locomotive behaviours as temperatures decreased. Since the average daily temperature decreased as the study progressed, it is possible that these behavioural changes represent the effects of the repetitive training regimen rather than the decrease in temperature.
Along with the physiological impacts of endurance exercise, dogs may have also experienced additional psychological symptoms throughout the conditioning period. Although acute exercise is associated with a positive effect on mood [40], the extreme exertion experienced by sled dogs may have variable effects. In humans, over-training is most often characterized by a decrease in mood, lack of motivation to exercise and chronic fatigue [5,41]. Although these symptoms are difficult to evaluate in working canines, it is likely that sled dogs have the potential to experience similar effects of over-training. Unfortunately, no previous research has defined behavioural signs of motivation to exercise in dogs. It is possible that the prevalence of postural changes or lunging forward on the gangline could be an indicator of anticipation or motivation to exercise when exhibited prior to running. The decrease in these behaviours seen throughout the conditioning period could potentially indicate a decrease in motivation; however, more research is needed to determine how these behaviours might be associated with other indicators of fatigue and over-training. Overtraining is additionally characterized in human athletes by a decrease in performance. However, sled dogs in the current study gained more lean muscle mass throughout the 12-week period [25], indicating physiological adaptations that would improve performance. This suggests that the dogs were not experiencing over-training in the present study. It is also possible that a decrease in these behaviours could suggest that dogs were becoming habituated to the training regimen, and therefore were less responsive as it progressed. Tracking observable behaviours prior to exercise may be useful for mushers or other sporting dog owners as an indication of motivation to exercise.

Conclusion
The findings of the current study suggest that Trp supplementation decreases agonistic behaviours in actively training sled dogs, while having no effect on activity or locomotion. Future research should continue to examine the use of Trp to decrease pre-run agonistic behaviours in working dogs, with a focus on dogs who have been pre-diagnosed with behavioural issues. Additionally, the reduction in activity and locomotive behaviours, such as lunging and changes in posture, following exercise was related to both to the intensity of a bout of exercise as well as to the repetitiveness of the training regimen. Short-term voluntary activity was related to the distance of the bout of exercise performed that day, while repetitive exercise caused a progressive decrease in locomotive behaviours during a pre-and post-exercise period. Future research should focus on assessing correlations between behavioural responses to repetitive exercise and physiological markers of muscle damage, over-training or fatigue. Overall, this research is the first to show the positive impact of an increased Trp: LNAA ratio on agonistic behaviour, which ultimately improves the workability of sled dogs and potentially decreases their risk of pain and injury. These results can be used to inform the development of diets and training programs designed to maximize the performance and success of sled dogs.
Supporting information S1 Table. Average percent of time (%) spent performing observed behaviours during 5-min pre exercise throughout 12 weeks of incremental conditioning for control dogs or tryptophan-supplemented (treatment) dogs. 1 Distance ran on days when behavioural observations took place. 2 Standard error of the mean; n = 10 for wks 0 to 2, 4 to 6 and 8 to 11; n = 9 for wk 7; n = 8 for wk 3. (DOCX) S2 Table. Average percent of time (%) spent performing observed behaviours during 5-min post exercise throughout 12 weeks of incremental conditioning for control dogs or tryptophan-supplemented (treatment) dogs. 1 Distance ran on days when behavioural observations took place. 2 Standard error of the mean; n = 10 for wks 0 to 2, 4 to 6 and 8 to 11; n = 9 for wk 7; n = 8 for wk 3.