Soluble lytic transglycosylase SLT of Francisella novicida is involved in intracellular growth and immune suppression

Francisella tularensis, a category-A bioterrorism agent causes tularemia. F. tularensis suppresses the immune response of host cells and intracellularly proliferates. However, the detailed mechanisms of immune suppression and intracellular growth are largely unknown. Here we developed a transposon mutant library to identify novel pathogenic factors of F. tularensis. Among 750 transposon mutants of F. tularensis subsp. novicida (F. novicida), 11 were isolated as less cytotoxic strains, and the genes responsible for cytotoxicity were identified. Among them, the function of slt, which encodes soluble lytic transglycosylase (SLT) was investigated in detail. An slt deletion mutant (Δslt) was less toxic to the human monocyte cell line THP-1 vs the wild-type strain. Although the wild-type strain proliferated in THP-1 cells, the number of intracellular Δslt mutant decreased in comparison. The Δslt mutant escaped from phagosomes during the early stages of infection, but the mutant was detected within the autophagosome, followed by degradation in lysosomes. Moreover, the Δslt mutant induced host cells to produce high levels of cytokines such as tumor necrosis factor-α, interleukin (IL)-6, and IL-1β, compared with the wild-type strain. These results suggest that the SLT of F. novicida is required for immune suppression and escape from autophagy to allow its survival in host cells.


Introduction
Francisella tularensis, a gram-negative, facultative intracellular bacterium causes tularemia in humans and animals [1]. Its reservoirs are rabbits and rodents, and it is transmitted to humans via routes such as arthropod bites and direct contact with infected animals [2]. F. tularensis is easily aerosolized and causes disease in humans at only 10 colony-forming units (CFUs) [3]. Therefore, F. tularensis is considered a potential biological weapon and, as such, is considered a category-A bioterrorism agent [4].F. tularensis comprises the subspecies tularensis (also called type A), holarctica (type B), mediasiatica, and novicida. Among them, only F. tularensis a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 subsp. tularensis and F. tularensis subsp. holarctica are highly virulent for humans and cause tularemia [4]. Although F. tularensis subsp. novicida (F. novicida) exhibits low virulence in humans, it is a facultative intracellular pathogen that replicates within macrophages and is pathogenic for mice. Moreover, F. novicida shares considerable homology with highly virulent subspecies. F. novicida is therefore widely used as a surrogate for the study of Francisella [5].
Francisella species are ingested through the pseudopod loops of macrophages and incorporated into vacuoles possessing endosomal markers [6,7]. Subsequently, the bacteria escape from phagosomes and replicate in the cytosol [8]. The Francisella pathogenicity island (FPI) is a gene cluster of approximately 30 kb encoding [16][17][18][19] open reading frames, which are required for the intracellular growth of Francisella [9]. In these FPI members, several genes are homologous to the core genes that encode the constituents of the type VI secretion system (T6SS) [10,11]. Deletion mutants of these genes persist in the host cell's cytosol. Therefore, a different mechanism related to intracellular survival may operate. Francisella is immunosuppressive through inhibition of the induction of inflammatory cytokines or melanization, and as a consequence, escapes the immune system, allowing it to survive in mammalian and arthropod hosts [12,13]. However, the mechanisms underlying immune suppression are unknown.
Lytic transglycosylases (LTs) degrade peptidoglycans by cleaving the β-1,4 bond between N-acetylglucosamine and N-acetylmuramic acid [14]. LTs, which are present in gram-negative bacteria, contribute to the remodeling of peptidoglycans and cell division. LTs are also required for the assembly of macromolecular complexes such as flagella, pili, and secretion systems larger than the size of peptidoglycan pores [15]. Although LTs are closely associated with the virulence of certain bacteria [16], the function of LTs in Francisella is unknown.
Here we constructed a transposon mutant library and determined that soluble lytic transglycosylase (SLT) is a novel pathogenic factor of F. novicida. We showed here that SLT was associated with intracellular growth and immunosuppressive activity, independent of the type VI secretion system (T6SS).

Bacterial strains and culture conditions
F. novicida U112 was obtained from the Pathogenic Microorganism Genetic Resource Stock Center (Gifu University). F. novicida was cultured aerobically at 37˚C in a chemically defined medium (CDM) [17] or in brain heart infusion broth (Becton, Dickinson and Company, Franklin Lakes, NJ) supplemented with cysteine (BHIc) [18] and containing 1.5% agar (Wako Laboratory Chemicals, Osaka, Japan). Listeria monocytogenes strain EGD was cultured in BHI broth. Bacterial concentrations were adjusted according to the optical density (OD 595 ) of the culture medium.

Cell culture
THP-1 cells (human monocytic cell line) and J774 cells (murine macrophage-like cell line) were grown in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO) supplemented with 10% heat-inactivated fetal bovine serum at 37˚C in an atmosphere containing 5% CO 2 .

Cytotoxicity assay
THP-1 cells (4 × 10 5 cells/well) were incubated in a 12-well or 48-well tissue culture plate with 200 nM phorbol myristate acetate (PMA) for 48 h. F. novicida strains were used at multiplicity of infection (MOI) of 0.5 or 0.01. The plates were then centrifuged for 10 min at 300 × g and incubated for the indicated time. Subsequently, the cells were washed three times with RPMI1640 medium, and extracellular bacteria were killed via exposure to gentamicin (50 μg/ ml) for 1 h. To measure lactate dehydrogenase (LDH), the cells were incubated in fresh medium at 37˚C for the indicated time. The release of LDH into supernatants was measured using an LDH Cytotoxicity Detection Kit (Takara Bio, Shiga, Japan).
Plasmid construction, transformation, and transfection S1 Table shows the primer sets and templates used to construct plasmids. PCR was performed using KOD-Plus-Neo (Toyobo, Osaka, Japan), and ligation was performed using Ligation High Ver. 2 (Toyobo) or an In-Fusion HD Cloning Kit (Takara Bio). Plasmids were used to transform F. novicida via cryo-transformation [19]. Briefly, bacterial cells were suspended in transfer buffer (0.2 M MgSO4, 0.1 M Tris acetate [pH 7.5]) with 1 μg of plasmid DNA. The bacterial cells were frozen in liquid nitrogen, thawed at room temperature, cultured in CDM, collected, and cultured on BHIc plates containing 50 μg/ml kanamycin or 2.5 μg/ml chloramphenicol.

Construction of a transposon mutant library
The transposon mutant library was constructed using the Ez-Tn5 transposon system (Epicentre, Madison, WI). The MCS of pMOD3 was digested using Hind III and EcoRI, and the kanamycin resistance cassette of pKEK1140 [20] was ligated to these sites to generate pMOD3-FtKm. The transposon moiety of pMOD3-FtKm was amplified using PCR, purified, mixed with transposase according to the instruction manual, and then used to transform F. novicida via cryo-transformation. Transformed bacteria were cultured on BHIc plates containing 50 μg/ml kanamycin.

Sequence analysis of transposon mutants
pMOD3 harbors the R6Kγ origin of replication of Escherichia coli. The genomes of F. novicida transposon mutants were purified using a PureLink Genomic DNA Mini Kit (Thermo Fisher, Waltham, MA) and digested with a combination of Xho I, BglII, EcoRI, SalI, NotI, and BamHI. The ends of the digested DNAs were then blunted using a DNA Blunting Kit (Takara Bio) and ligated using Ligation High Ver. 2 (Toyobo). The ligated DNA was used to transform One Shot PIR1 Chemically Competent E. coli (Thermo Fisher). The transformed E. coli were selected for kanamycin resistance, and the plasmid DNAs were purified. Sequence analysis was performed using the primer described in the instruction manual for the Ez-Tn5 transposon system.

Construction of F. novicida mutants
The dotU homolog (FTN_1316) deletion mutants (ΔdotU) of F. novicida were generated through group-II intron insertion using the TargeTron Gene Knockout System (Sigma-Aldrich) modified for Francisella species [20], as previously described [21]. The slt (FTN_0496) deletion mutant (Δslt) was generated via homologous recombination using the Francisella suicide vector pFRSU [21]. The upstream and downstream regions of slt (1.5 kbp each) were cloned into the BamHI site of pFRSU to generate pFRSU-slt. pFRSU-slt (1 μg) was used to transform F. novicida, and the cells were cultured on BHIc plates containing 50 μg/ml kanamycin. Isolated bacteria were cultured in CDM without antibiotics for 6 h and then plated on BHIc plates containing 5% sucrose. Deletion of the slt gene was confirmed via PCR.

Intracellular growth assay
THP-1 cells (4 × 10 5 cells/well) were incubated in a 48-well tissue culture plate with 200 nM PMA for 48 h. F. novicida strains were added at MOI = 1. Plates were centrifuged for 10 min at 300 × g and incubated for 1 h at 37˚C. The cells were washed three times with RPMI1640 medium, and extracellular bacteria were killed using gentamicin (50 μg/ml) treatment for 1 h. The cells were incubated in fresh medium at 37˚C for the indicated time. Cells were incubated in the presence of 5 mM 3-methyladenine (3-MA) (Wako Laboratory Chemicals) for the indicated time. To measure intracellular growth, the cells were washed with phosphate-buffered saline (PBS), and then lysed with 0.1% Triton X-100 in CDM. The number of CFUs on BHIc plates was determined via plating serial dilutions of cultures.

Fluorescence microscopy
THP-1 cells (4 × 10 5 cells/well) on 12-mm glass coverslips in 48-well tissue culture plates were incubated with 100 nM PMA for 48 h. THP-1 cells were infected with F. novicida strains and incubated for the indicated times. To visualize lysosomes, cells were stained with LysoTracker Red (Thermo Fisher) according to the source's instruction manual. To detect LC3, cells were fixed with 4% paraformaldehyde at room temperature for 30 min and permeabilized with 100 μg/ml digitonin for 5 min. Cells were treated with an anti-LC3 antibody (PM036, 1:100, Medical & Biological Laboratories, Nagoya, Japan) and stained using an Alexa Fluor 555-conjugated anti-rabbit IgG (ab150078, 1:1000, Abcam, Cambridge, UK). To detect lysosomalassociated membrane protein 1 (LAMP-1), cells were fixed using the PLP Solution Set (Wako Laboratory Chemicals) containing 5% sucrose for 1 h at 37˚C and then permeabilized using cold methanol for 10 s. The cells were treated with an anti-LAMP-1 antibody (ab25245, 1:100, Abcam) and stained with FITC-conjugated anti-rat IgG (1:1000, Abcam). A FluoView FV100 confocal laser scanning microscope (Olympus, Tokyo, Japan) was used to acquire images of the cells.

T6SS secretion assay
The T6SS secretion assay was performed according to a published method [22]. To delete the endogenous β-lactamase gene (bla, FTN_1072), upstream and downstream sequences of bla (1.5 kbp each) were cloned into the BamHI site of pFRSU to generate pFRSU-bla, which was used to transform wild-type, Δslt, or ΔdotU mutants to generate Δbla, ΔsltΔbla, and ΔdotUΔbla mutants. The iglC gene (FTN_1322) of F. novicida encoding the T6SS effector protein was cloned using pOM5, as described above, and the ampicillin resistance gene (ampR) derived from pCMV-HA-N (Takara Bio) was cloned downstream of IglC to generate pOM5-Igl-C-AmpR. To express the fusion protein of IglC and AmpR (IglC-AmpR), pOM5-IglC-AmpR were used to transform the Δbla, ΔsltΔbla, or ΔdotUΔbla mutant. THP-1 cells (4 × 10 5 cells/ well) on 12-mm glass coverslip in a 48-well tissue culture plate were incubated with 100 nM PMA for 48 h. F. novicida strains were infected. After incubating for the indicated times, THP-1 cells were treated with the β-lactamase substrate CCF2 AM (Invitrogen, Waltham, MA).
CCF2 AM (green fluorescence) was digested by IglC-AmpR that was secreted into the cytosol of THP-1 cells, and β-lactamase activity was detected as blue fluorescence.

ELISA
THP-1 cells (4 × 10 5 cells/well) were incubated in a 48-well tissue culture plate with 100 nM PMA for 48 h and then infected with F. novicida strains, L. monocytogenes, or treated with 100 ng/ml LPS derived from E. coli (O55:B5). After incubation for 6 h, the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β in the supernatants were measured using an ELISA MAX Standard Kit (Biolegend, San Diego, CA) according to the manufacturer's instructions.

Statistical analysis
Multiple comparisons using the Bonferroni/Dunnett method or the Student's t test were used to evaluate the significance of differences between groups. P <0.05 indicates a significant difference.

Identification of genes required for the cytotoxicity of F. novicida
To identify novel virulence factors of F. novicida, we generated an F. novicida transposon mutant library. F. novicida is cytotoxic to the human monocyte cell line THP-1, and cells consequently detach from the culture plate. We therefore performed microscopic observation to screen for mutant strains of F. novicida lacking cytotoxic activity. Among 750 transposon mutants, 11 mutants were identified as less cytotoxic. To confirm these findings, we performed LDH assays. Compared with the wild-type strain, these mutants caused decreased release of LDH from THP-1 cells (Fig 1). To identify the genes responsible for cytotoxicity, the transposon insertion sites of the mutant strains were sequenced (Table 1). Here we focused on slt (FTN_0496) encoding SLT and analyzed its functions.

Effect of SLT on intracellular growth and cytotoxicity
To evaluate the effect of SLT on cytotoxicity, we constructed the slt deletion mutant (Δslt) of F. novicida via homologous recombination. The deletion and transposon mutants of slt grew at rates equivalent to those of the wild-type and the complemented (Δslt/slt) strains when grown in BHIc medium (Fig 2A). The comma-shape of the Δslt mutant differed from that of the wildtype strain (S1 Fig). The Δslt mutant induced decreased LDH release compared with that of the wild-type strain, whereas the complemented strain released amounts of LDH comparable to that of the wild-type strain (Fig 2B). Deletion of the dotU homolog (FTN_1316, ΔdotU), a gene encoding a component of the T6SS apparatus, yielded reduced LDH release. To investigate the mechanism of cytotoxicity, we measured the intracellular growth of the Δslt mutant in THP-1 cells. The wild-type strain proliferated intracellularly. In contrast, the number of intracellular Δslt mutant significantly decreased compared with that of the wild-type strain. The number of intracellular ΔdotU mutant was maintained (Fig 2C). We next visualized intracellular bacteria using GFP expressing F. novicida strains. Intracellular wild-type and Δslt mutant were observed 12 h after infection (S2 Fig). However, the larger and spherical shape of the intracellular Δslt mutant differed from that of the wild-type strain (Fig 2D). Because F. novicida exhibits low virulence in humans and is pathogenic in mice, we investigated the intracellular growth of F. novicida in the murine macrophage-like cell line J774. Similar to THP-1 cells, the intracellular growth of Δslt mutant was reduced in J774 cells compared with the wild-type strain (S3 Fig). These results suggested that slt was required for the cytotoxicity and intracellular growth of F. novicida.

Involvement of SLT in escape from phago-lysosomes
To gain further insights into the role of slt in the intracellular growth of Francisella, we observed the intracellular behavior of the Δslt mutant in THP-1 cells. THP-1 cells were   infected with GFP-expressing F. novicida strains and observed using confocal microscopy. At first, the ability of F. novicida to escape from phago-lysosome at the early stages of infection (0.5 h, 1 h, and 2 h after infection) was observed using Lysotracker. Colocalization of Lysotracker and the wild-type or Δslt strain was not observed (S4 Fig), suggesting that the wild-type and Δslt strains escaped from the phago-lysosomes.

Involvement of SLT in recognition by autophagy
We next observed the intracellular behavior of the bacterial strains during the late stages of infection. The wild-type strain proliferated intracellularly from 2 h to 24 h after infection. Consistent with the numbers of intracellular bacteria (Fig 2C), the number of Δslt mutant increased until 12 h after infection, although the number of bacteria decreased 24 h after infection ( Fig 3A). The relationship between F. novicida strains and autophagy was next evaluated.
In THP-1 cells infected with the wild-type strain, colocalization of bacteria with the autophagosome marker LC3 was infrequent (Fig 3A). Specifically, 20% of wild-type bacteria colocalized with LC3 after 2 h, and <5% of bacteria were associated with LC3 6-24 h after infection. In contrast, the Δslt mutant colocalized with LC3 throughout infection ( Fig 3A) at frequencies ranging from 50%-70% (Fig 3B), indicating that SLT was required for F. novicida to evade sequestration and destruction via autophagy. To confirm the association between the Δslt mutant and autophagy, THP-1 cells were treated with the autophagy inhibitor 3-MA and infected with F. novicida strains. In the presence of 3-MA, the intracellular growth of the Δslt mutant significantly increased 48 h after infection (Fig 3C).

Effect of SLT on the escape of bacterial cells from lysosomes
To determine whether the Δslt mutant was digested by lysosomes after capture by autophagosomes, THP-1 cells were infected with the Δslt mutant, and lysosomes were visualized using an antibody against LAMP-1 (Fig 4A). When THP-1 cells were infected with the wild-type strain, intracellular bacterial cells were observed, but few of them colocalized with LAMP-1 (Fig 4A  and 4B). In contrast, the Δslt mutant was observed 12 h after infection, although the number decreased 18 h and 24 h after infection (Fig 4A). The ratio of Δslt mutant that colocalized with LAMP-1 increased from 10% to 20% 12-24 h after infection (Fig 4B). These results suggested that F. novicida escaped degradation by lysosomes in an SLT-dependent manner.

Relationships between SLT and T6SS
LTs play an important role in forming the T6SS in E. coli [23]. Therefore, we evaluated the influence of SLT on the activity of the T6SS. To express the T6SS effector protein IglC fused with β-lactamase (IglC-AmpR), iglC and ampR encoding β-lactamase were cloned into a plasmid (iglC-ampR). The β-lactamase activity of the secreted fusion protein was detected using the β-lactamase substrate CCF2 AM (S5 Fig). Wild-type F. novicida possesses an endogenous β-lactamase gene (bla), and consequently, CCF2 AM (green fluorescence) was digested and blue fluorescence was observed. Although β-lactamase activity was not detected when the Δbla mutant was infected, the activity was detected when the Δbla mutant containing iglC-ampR (Δbla/iglC-ampR) was infected. The Activity was not detected when the Δbla-ΔdotU doublemutant containing iglC-ampR (ΔblaΔdotU/iglC-ampR) was infected. These results suggest that IglC-AmpR was secreted into the cytosol of THP-1 cells through the T6SS. In contrast, the activity was detected when the Δbla and Δslt double-mutant containing iglC-ampR (ΔblaΔslt/ iglC-ampR) was infected, indicating that the T6SS was active and effective in the Δslt mutant.

Relationship between SLT and the immune response
Francisella induces immune suppression, and the immune responses to Francisella are maintained at relatively low levels compared with those of other bacteria such as Listeria monocytogenes [24]. To assess the effect of slt on immune responses, we measured the induction of Functional analysis of Francisella soluble lytic transglycosylase cytokines produced by THP-1 cells. The induction of TNF-α, IL-6, and IL-1β was maintained at relatively low levels compared with infection with L. monocytogenes or treatment with LPS. In contrast, the Δslt mutant induced high levels of cytokines compared with cells infected with the wild-type and complemented strains (Fig 5). These results indicated that F. novicida induced immune suppression through an SLT-dependent pathway.

Discussion
The molecular mechanisms underlying the pathogenicity of Francisella species are poorly understood. Here we developed a transposon mutant library of F. novicida to isolate mutants that were less cytotoxic to THP-1 cells. Among 11 mutants, major facilitator superfamily (MFS) transport protein, intracellular growth locus protein B, and gamma-glutamyl transpeptidase had already been reported as pathogenic factors of Francisella [10,25,26]. Two strains among the rest of seven had transposon insertions in different positions of the slt gene. We reasoned therefore that slt was deeply involved in cytotoxicity and focused on the gene encoding SLT. LTs are lytic enzymes of peptidoglycan which create spaces within the peptidoglycan to insert a protein complex such as a secretion system, flagella, or pili into the peptidoglycan or outer bacterial membrane [27][28][29]. In certain bacterial species, the expression of LTs are upregulated during infection [30]. Moreover, LTs are closely associated with the pathogenicities of bacteria such as Brucella abortus or Helicobacter pylori [31,32]. However, the function of the LTs of Francisella is not well understood.
Francisella are ingested through the pseudopod loops of macrophages and taken up into spacious vacuoles possessing endosomal markers [6,7]. Subsequently, the bacteria escape from the phagosomes and replicate in the cytosol [8]. During the late stages of infection, the bacteria re-enter the autophagosomes [33,34]. F. tularensis subsp. holarctica LVS is sequestered by autophagy after escape from phagosomes to the cell cytosol, but the bacteria escape degradation and acquire amino acids from degraded proteins to replicate in LAMP-1 positive autophagosomes, called Francisella containing vacuoles [33]. However, infection with highly virulent F. tularensis subsp. tularensis SchuS4 interferes with the autophagic pathway, and only replication-deficient or damaged cytosolic bacteria are captured by autophagosomes and then degraded through the ubiquitin-SQSTM1-LC3 pathway [34]. We showed here, similar to F. tularensis subsp. tularensis SchuS4, that most F. novicida replicated within the cytosol of THP-1 cells, and only 1%-20% of the bacteria colocalized with the autophagosome marker LC3. The Δslt mutant grew intracellularly until 12 h after infection of THP-1 cells, but the numbers of intracellular bacteria decreased 24 h and 48 h after infection. Although the Δslt mutant did not colocalize with acidic organelles stained with LysoTracker during the early stages of infection (0.5 h-2 h), the mutant colocalized with the autophagosome marker LC3 and the lysosome marker LAMP-1 during the late stages of infection (12 h-48 h after infection). Moreover, the inhibitor of autophagy 3-MA partially restored the intracellular growth of the Δslt mutant. These results might suggest that the Δslt mutant was able to escape from phagosomes, but the bacteria were damaged during their replication in the cell cytosol and were captured by autophagosomes followed by degradation. Meanwhile, the autophagy inhibitor 3-MA failed to completely restore the intracellular growth of Δslt mutant. In addition, only 50% and 20% of the Δslt mutant colocalized with LC3 and LAMP-1, respectively. Therefore, we were unable to exclude the possibility that most of the Δslt mutant failed to escape from their initial phagosomes and were consequently degraded within them. Nevertheless, these findings suggest that SLT is required for the intracellular replication of F. novicida.
The Δslt mutant exhibited an increased ability to induce the production of inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Francisella species suppress inflammatory responses, enabling these bacterial pathogens to survive in the host [12]. Although induction of inflammatory cytokines via Francisella infection is suppressed compared with other bacteria such as L. monocytogenes [24], the detailed mechanisms underlying immune suppression are unknown. Here we showed that the induction of TNF-α and IL-6 production by THP-1 cells infected with wild-type F. novicida was suppressed compared with that of L. monocytogenes Functional analysis of Francisella soluble lytic transglycosylase infection or LPS treatment. However, induction was restored when the cells were infected with the Δslt mutant. The production of inflammatory cytokines such as TNF-α or IL-6 is generally induced by recognition of bacterial components by Toll-like receptors (TLRs) followed by the nuclear translocation of transcription factors such as NF-κB [35]. In host cells infected with Francisella, the production of inflammatory cytokines such as TNF-α or IL-6 is induced by recognition of Francisella by TLR2, followed by the recognition of Francisella DNA by TLR 9 [36]. These results suggest that F. novicida suppresses TLR2 and TLR9 signaling through SLTdependent mechanisms.
The induction of IL-1β production was restored in host cells infected with the Δslt mutant. For maturation and release of IL-1β, stimulation of intracellular receptor inflammasomes is required [37]. After expression of the IL-1β precursor, through TLR signaling in host cells infected with Francisella, the intracellular recognition of Francisella DNA by the AIM2 inflammasome is required for the conversion of the IL-1β precursor to its mature form [38]. However, the activation of inflammasomes by Francisella is suppressed compared with that of cells infected with other bacteria [39]. Here we showed that the induction of IL-1β increased compared with cells infected with wild-type bacteria, although the intracellular growth of the Δslt mutant was relatively decreased. These results suggest the possibility that SLT contributes to the suppression of inflammasome signaling.
LTs play an important role in constructing the T6SS in E. coli [23]. It is therefore possible that intracellular growth or immune suppression by F. novicida requires the T6SS. However, the T6SS was active in the Δslt mutant. Further, the intracellular behavior of the T6SS apparatus mutant (ΔdotU) differed from that of the Δslt mutant, and the number of intracellular bacteria was constant during the late stages of infection (12 h-24 h after infection). These results indicated that the inability of the Δslt mutant to undergo intracellular growth and mediate immune suppression was independent of the T6SS. In bacteria such as Acinetobacter, SLT is required to form type-IV pili, which is associated with bacterial pathogenicity [29]. In F. novicida, proteins associated with pathogenicity such as peptidase or chitin-binding proteins are secreted from the Type-IV pili apparatus [40]. These findings suggest that such secreted proteins may contribute to the intracellular growth or immunosuppressive activity of F. novicida.
Most recently, it was reported that the SLT of F. novicida is associated with bacterial pathogenicity for mice [41] through an unknown mechanism. Here we showed that SLT was involved in the intracellular growth of F. novicida and in its immunosuppressive activity. These findings suggest that in mouse or human macrophage cells, F. novicida may suppress the induction of immune responses in an SLT-dependent manner, allowing its escape from immune functions such as recognition and degradation via autophagy.
In conclusion, we identified SLT as a new pathogenic factor of F. novicida. However, the detailed mechanisms of SLT that contribute to intracellular growth and immunosuppressive activity remain to be identified. Highly pathogenic Francisella species such as F. tularensis subsp. tularensis harbor slt. Therefore, it is critically important to determine the function of SLT, which may provide a basis for understanding the mechanism through which Francisella exerts its pathogenicity.