Sign and goal tracker rats process differently the incentive salience of a conditioned stimulus

Sign and goal tracker animals show different behavioral patterns in response to conditioned stimuli, which may be driven by different neural circuits involved in processing stimuli. Here, we explored whether sign and goal-tracker profiles implicated different brain regions and responses to incentive salience of stimuli. We performed three experiments using male Wistar rats. Experiment 1 showed that lesioning the medial prefrontal cortex increased the prevalence of the goal-tracker phenotype. Experiment 2 assessed the developmental trajectory of the salience incentive attribution to a conditioned stimulus, showing that increased incentive salience of stimuli increased the prevalence of the sign-tracker phenotype in mature, but not preadolescent rats. In experiment 3, the functional impact of the medial prefrontal cortex circuits was analyzed with a latent inhibition procedure. Sign tracker rats showed a reduced latent inhibition to stimuli previously exposed when compared to goal tracker or intermediate rats. The overall results of this study highlight a key role of the medial prefrontal cortex for sign tracking behavior. The expression of sign and goal tracker phenotypes changed after lesion to the medial prefrontal cortex (experiment 1), differed across development (experiment 2), and showed differences in the attentional processes to previously exposed stimuli, as preexposure to CS was ineffective in sign tracker animals (experiment 3). These data indicate that the responses to the incentive salience of stimuli in sign tracker and goal tracker profiles are likely driven by different neural circuitry, with a different role of prefrontal cortical function.


Introduction
The incentive salience of a conditioned stimulus (CS) helps an animal with predicting the arrival of an unconditioned stimulus (US). Associative learning theories include incentive salience as an important variable [1][2][3]. However, animals diverge in the way in which they attribute salience to a CS, as observed in autoshaping procedures. These individual differences have allowed the observation of different patterns of behavior related to incentive salience attributed to a CS, termed goal and sign tracking [4]. a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 Goal-and sign-tracker (GT and ST) animals show different relationships with CS. While GT animals show a behavior focused on the goal or approaching the place where the reward will be released, ST animals focused on the cue [5][6][7]. This classification, despite being simplistic, assumes different behavioral patterns. Flagel et al. [8] showed that animals bred to enhance response (bred High Responders; bHR) display a ST pattern of behavior, and this pattern correlated with lower levels of anxiety than bred Low Responders (bLR, which show a GT profile), higher levels of aggressive behavior, impulsivity and addiction-related traits [8]. In addition, electrochemical studies have shown higher levels of dopamine release in nucleus accumbens (NAc) after CS presentation in ST animals [7]. These differences could impact risk for mental illnesses [9][10][11]. In a recent paper, Flagel et al. [12] described the different performance of low/ high response rats in an autoshaping procedure after cocaine exposure during adolescence, a critical developmental period for brain regions implicated in these behaviors. Cocaine administration had a higher effect on GT animals compared to ST animals, suggesting GT rats are more sensitive to stimulants than ST rats during adolescence. Similarly, we [10] reported that adult ST animals with a neonatal ventral hippocampal lesion (NVHL) showed a lower response to CS than sham animals, indicating a reduced motivational response to CS in this rodent model of altered medial prefrontal cortex (mPFC) developmental trajectory [13]. This fact is interesting since NVHL procedure disrupts the normal dopamine modulation of corticolimbic circuits [14]. In addition, NVHL animals were less sensitive to dopamine agonists such as quinpirole. Thus, GT and ST rats are likely different in the manner their mPFC processes information.
The mesolimbic dopamine system is essential for attribution of incentive value to an US. Additionally, this neurotransmitter is an important target in major brain diseases such as attention deficit hyperactivity disorder (ADHD), schizophrenia, and substance abuse [15][16][17][18]. The NAc is an essential part of the mesolimbic system that receives dopaminergic fibers from ventral tegmental area and is modulated by afferences from the hippocampus, amygdala, and prefrontal cortex among other structures [19][20][21][22][23]. The NAc is necessary for attributing incentive salience to reward cues [24], and mPFC modulates dopamine levels in the NAc by means of excitatory glutamatergic projections. It is possible that the mPFC projection to the NAc modulates attribution of incentive salience to a CS, therefore determining the expression of ST behavior. If so, this structure should play out more strongly role in ST animals than GT in autoshaping procedure. In order to analyze several aspects of the mPFC involvement in ST and GT animals, experiment 1 assessed whether mPFC lesions differentially affected ST and GT behaviors. Experiment 2 analyzed the changes in salience incentive attribution to a CS during a critical period of mPFC development. If mPFC was involved in ST/GT behavior, behavioral patterns would be different as mPFC matured. Lastly, experiment 3 tested the functional impact of mPFC circuits by assessing attentional processing in ST and GT rats with latent inhibition of a tone-shock association. Latent inhibition allows us an indirect measuring of attentional processes to future CS and it has been recently related to mPFC function [25].

Ethics statement
All procedures used in the present study were in compliance with the regulations of the European Community Council Directive 2010/63/EU and following the Spanish regulations (R.D 53/02013) for the use of laboratory animals. An ethical commission of University of Seville and Institutional Animal Care and Use Committee (Sanidad Animal, Junta de Andalucía) supervised and approved all the procedures and all protocols used in this specific study. The code of the supervision report is: 31/08/2016/153. Previous studies have showed that the mPFC is involved in controlled processes and goaldirected behavior [25,26] that would imply an increased attention to a CS. López et al. [10] found a decrease in motivational processes in adult animals with a NVHL, a procedure that impairs adolescent prefrontal cortex maturation. To determine whether the mPFC is involved in salience incentive processing, we studied the effect of mPFC lesions in an autoshaping procedure. Autoshaping describes the orientation-approach movements to a CS that appears prior to the US [27]. If mPFC is involved in incentive salience of a CS, a behavioral change should be observed in ST, but not in GT rats.

Materials and methods
Animals. Fifty adult male Wistar rats (300-350g) were obtained from the Centro de Producción y Experimentación Animal (Universidad de Sevilla). Animals were housed individually in their home boxes with 12:12 hours light/dark cycle, and all tests took place during the cycle's light period. All animals had access to food and water ad libitum during the experiment.
Surgery. Under deep isoflurane anesthesia (2-5% in air, flow rate 1 l/min, 5% induction; 2% maintenance; McKinley type 2, Everest), rats were placed in a David Kopf stereotaxic instrument. The mPFC was injured following coordinates 4.7; 4.2; 3.7 mm anterior, ±0.6 mm lateral, and 4.0 mm ventral from Paxinos and Watson [28]. This lesion was made by injecting NMDA (1 mg in 0.1 ml phosphate buffered saline, PBS 0.1 M; see [29]) into the brain through a 10 μl Hamilton syringe (Model 1701 RN). The amount of NMDA solution injected at each site was 0.25 μl, at a rate of 0.05 μl/min. The needle was left in place for an additional minute after the infusion to allow the diffusion of the solution into the tissue. No unexpected adverse effects were observed 48 h after the surgery. Sham animals received a similar manipulation, but saline solution was injected instead of the drug.
Histological analysis. Assessment of mPFC lesion placement. Upon completion of behavioral testing, rats were deeply anesthetized and perfused transcardially with a fixative solution (10% formalin in phosphate buffer 0.1 M, pH 7.4). The brains were then removed from the skull and placed in 10% formalin and buffered for 3-4 days. Next, the brains were cut with a microtome at the coronal plane at 500020μm thickness oriented according to the atlas of Paxinos & Watson [28] and Nissl stained for histological analysis. Specifically, we stained the tissue with cresyl violet method. This allows to determine the extent of mPFC lesion using an image processing software (ImageJ. NIH, USA, http://rsb.info.nih.gov/ij/). Animals included in this study showed lesions between 49-85% of damage in mPFC (Fig 1), and without significant damage to the adjacent structures.
Behavioral testing: Pavlovian conditioning. Four standard boxes from MED Associates (21.1cm x 20.5 cm area, 29.2cm high; Med Associates. St. Albans. VT) were used for Pavlovian Conditioning training. The test chambers were located inside a soundproof test room with constant light and temperature conditions. Methods and procedures were similar to Lopez et al. [10]. Briefly, each chamber was equipped with a feeder located in the middle of the right wall and 3 cm from the bottom grid. The retractable lever was located to the left of the feeder. To increase salience, the lever was illuminated with a white LED. On the opposite wall, at the same height as the feeder there was a monitoring sensor "Nose Poke". A pellet dispenser provided standard taste pellets into the feeder that served as reinforces. An infrared sensor located inside the receptacle (approximately 1.5 cm from the base of the feeder) recorded each time the animal entered the feeder (when the lever was available). A background white light located on the opposite side to the feeder was on during the whole test. Contacts with the lever were recorded as "Lever Press"; contacts with feeder were recorded as "Nose Feeder".
Boxes were programmed using Med Associates software. This software recorded the following responses during the whole test: (1) number of lever presses, (2) latency to lever press in the first response for each test, (3) number of feeder entries while illuminated lever was available, (4) latency of the first entry into the feeder when CS was presented, and (5) number of contacts with nose feeder. All test sessions took place between 9:30 and 11:30 a.m. Before the pre-training phase, animals received 5 pellets of the same kind as those used during the test as US in their home boxes for three consecutive days in order to prevent neophobia to the pellets. There were two pre-training sessions during two consecutive days. Thirty pellets were randomly administered for 45 minutes in those training days. The pavlovian conditioning sessions consisted on presenting an illuminated lever (CS) for eight seconds, 25 trials per session. Immediately after CS presentation, the lever retracted and a 45 mg pellet (MLab Rodent Tablet, manufactured by Nottingham University) was provided into the feeder. CS were presented in a 90s variable interval schedule (60s the lowest intertrial interval and 120s the highest). Training sessions continued for four days, allowing classification of rats as ST, GT, or Intermediate (Int) using the Pavlovian Conditioned Approach Index Score (PCA) [30]. This index uses the number of responses to both the lever and the feeder on a rating from -1 to 1. Scores lower than -0.5 indicate a GT phenotype, while ratings higher than 0.5 indicate a ST phenotype. Scores between -0.5 to 0.5 are defined as Int phenotype.
Data analysis. In order to analyze the PCA response and nose poke response in sham and mPFC groups, we used parametric T-student test. When it was required, for instance lever press or magazine entries, an ANOVA test (repeated measures) was used including sham and mPFC lesion groups. The different distribution of phenotypes by groups (sham and mPFC) was analyzed with Friedman and Mann-Whitney tests. Significant level was established at p < .05.

Results
Rats were trained in autoshaping procedure for four sessions. To evaluate the profiles of shamand mPFC-lesioned groups, we determine their PCA index. Both groups consumed all pellets in each session, and no motor problems were observed after mPFC lesion. In fact, we did not find differences in the number of nose pokes as a control of animal activity (all T (48) <1.4, all p>0.16; Fig 2A). However, a thorough analysis showed that mPFC animals displayed a lower rate of responses in all groups than sham animals. They showed a significant less number of press lever and magazine entries (F(3, 144) = 5,59 and 4,52; p<0.01 for lever press and magazine entries respectively; Fig 2B and 2C).
In order to analyze the global behavior, we used PCA index. This index provided a global measure of lesioned and sham animals performance. We observed a similar activity in PCA for sessions 1 and 2; i.e., the distribution of responses was not different between groups (Mann-Whitney, p = 0.11 and p = 0.30 for sessions 1 and 2; Fig 2D). However, this distribution changed with training. Although sessions 3 and 4 showed similar performances as sessions 1 and 2 in sham rats, mPFC lesioned rats showed a predominant GT behavior (Friedman, p<0.01; Fig 2D), along with a reduced proportion of ST animals (Fig 3; Mann-Whitney, both p<0.01). Sham animals displayed a normalized distribution, where the largest numbers of animals are closer to the mean (Int animals), whereas mPFC-lesioned animals showed a distribution displaced to a GT phenotype (Fig 3).

Experiment 2
Experiment 1 showed that animals with a mPFC lesion reduced lever-press behavior when CS was available. The maturation of the mPFC during adolescence is a key process for controlling attentional mechanisms [31,32]. If incentive salience of a CS could be related to attentional processes dependent of mPFC maturation, the ST/GT distribution would be different prior to the adolescent critical period compared to a later stage. In order to deepen in this issue, a program of food deprivation was started. Several authors have suggested that some physiological states like hunger could, among other effects, increase incentive salience of food and their associated cues by modulating the motivational value [16,33,34]. This increased motivational could produce an attentional bias towards food-related cue (i.e. [35,36]) and a change in the distribution of ST/GT population. Thus, we analyzed performance in an autoshaping procedure during different motivational states comparing different phases of mPFC maturation, such as pre-adolescent (4 weeks of age) and late adolescent (9 weeks of age) stages.

Materials and methods
Animals. Eighty-four male Wistar rats were used in this experiment. However, twelve of them were removed from the experiment because they did not get the food from the magazine at least 90% of the trials. After the pavlovian conditioning phase, rats were divided in three groups by age. N = 33 (GT n = 7, ST n = 14, Int n = 12) for 4-5 week-old animals (average weight of 150 g; range 130-160 g) and N = 39 (GT n = 7, ST n = 18, Int n = 14) for 8-9 weekold rats (average weight 295 g; range 270-345 g). As in experiment 1, animals were housed individually in their home boxes and all animals had access to food and water ad libitum during first phase of the experiment.
Behavioral testing: Pavlovian conditioning. Experiment 2 included three phases of autoshaping procedure. Phase A was a Pavlovian conditioning similar to experiment 1. Phase B included the same Pavlovian conditioning than in Phase 1, but in this case the rats were under a food deprivation schedule. After the initial training in Phase A, rats were deprived of food for three consecutive days until they reached 90% of their weight. Once the targeted weight was reached, Phase B started, consisting on the same pavlovian training received in the previous phase (Phase A), under the same criteria. Deprivation conditions were maintained for the four test sessions and therefore rats were kept at 90% of body weight. After four sessions in Phase B, animal returned to initial conditions and they were trained for four more additional sessions in a Phase A-like (termed Phase A'). Med Pc software recorded the same responses as in experiment 1.
Data analysis. Similar to experiment 1, we used an ANOVA test and Tukey post-hoc test. In addition, a repeated measures test helped us to analyze the progressive change across the experiment in all groups.

Results
As in experiment 1, animals learnt to obtain food from the magazine in the Skinner boxes. The PCA index allowed classifying rats into GT, ST, and Int profiles, depending on their behavior in session 4 (Phase A). A repeated-measures analysis of PCA index revealed a significant interaction of sessions and phenotype in pre-adolescent (F (6, 90) = 4.92; p<0.01; Fig 4A) and late adolescent groups (F (6, 105) = 3.62; p<0.01; Fig 4B) during Phase A. The main effect of session was significant in both GT and ST rats (all p<0.05 for pre-adolescent and late adolescent groups), but not in Int (p>0.3 for pre-adolescent and late Int groups). There was also no differences between pre-adolescents and late-adolescent performance (F(6, 198) = 0.92; p = 0.48).
Once Phase A ended, animals were food deprived to reach 90% of body weight, which took about three days. We then explored the changes in behavior produced by increasing motivation with a food deprivation program (Phase B). ST rats displayed a similar PCA index in Phase A and B (F (3, 39) = 2.33; p = 0.08; Fig 4A), although they increased lever press responses compared to Phase A (repeated measures including Phase A and B F (7, 91) = 6.86; p<0.01; Fig  4A). Magazine entries were similar in Phase A and B (F (7, 91) = 1.85; p = 0.08; Fig 4B). However, GT animals showed a shift in the response to the CS when the motivation increase program started. They increased lever pressing (F (7, 35) = 7.10; p<0.01) and magazine entries (F (7, 35) = 6.86; p<0.01; Fig 4B and 4C respectively) in Phase B compared to Phase A. This increase in both responses suggests a high level of overall activity, shifting their PCA index from GT to Int. Finally, the Int group displayed a displacement in the response ratio, approaching a ST pattern (F (3, 33) = 14.15; p = 0.01; Fig 4A). Thus, increasing incentive salience with food restriction resulted in increased activity but no clear phenotype change in immature rats.
Once finished Phase B animals started Phase A again (Phase A'). We did not find differences between this phase and the last session of Phase B in PCA index (F(3,96) = 1.31; p = 0.27; Fig 4A) in any groups. This is, animals maintained the same profile in Phase B and the new phase A (Phase A').
Late adolescent group. Late adolescent rats performed similar to pre-adolescent rats for all three phenotypes in Phase A. After 4 sessions, we classified the rats as ST, GT or Int. ST rats showed a high number of lever press responses and low number of magazine entries (both F (3,48) >6.18; both p<0.01; Fig 4B and 4C). GT did the opposite, as expected, (F (3, 18) = 3.37; p = 0.041 and F (3,18) = 1.38; p = 0.28 for magazine and press lever response, respectively; Fig 4B and 4C). Int rats did not show any change across sessions (both F (3,39) >0.61; both p>0.47; Fig 4B and 4C).
Phase B showed a clear difference between pre-adolescent and late adolescent rats for the GT group. While younger animals did not decrease magazine entries, late adolescent rats showed a ST phenotype as they increased lever press responses (F (7, 42) = 24.19; p<0.01; Fig  4B) and reduced magazine entries (F (7, 42) = 4.59; p<0.01; Fig 4C) compared to Phase A. Fig  4A shows the PCA index shift in GT (F (3, 18) = 5.66; p<0.01). In contrast, ST showed a similar phenotype compared to Phase A (F (3, 51) = 4.59; p<0.15) after motivation increase program; that is, high lever press response and low magazine entries response. Int rats showed a similar pattern as pre-adolescent rats, approaching a ST phenotype (F (3, 39) = 12.22; p<0.01; Fig 4A) as evidenced by a lever press increase and a magazine entry decrease (F (7,91) >6.29; both p< 0.01; Fig 4B). Similar to pre-adolescent animals, late adolescent group did not show any shift in the new Phase A (Phase A') in regard to the end of Phase B (F(3,111)

Experiment 3
Experiments 1 and 2 have revealed several differences between ST and GT animals, including dependence on intact mPFC, developmental state, and incentive salience state. These profiles could result in a different response to preattentive sensory stimuli, as we recently showed using a prepulse inhibition procedure [10]. In fact, mPFC sends a dense glutamatergic projection to nucleus accumbens, a structure that is itself necessary for LI expression. In addition, several studies have analyzed the involvement of mPFC in LI finding a clear increase of LI after mPFC lesion. Since mPFC showed a different involvement in ST and GT, we wondered if the different behavioral profiles could show any difference in LI processes. With this aim, in experiment 3 we analyzed the attention to a future conditioned stimulus in GT and ST animals using a latent inhibition (LI) procedure.

Materials and methods
Animals. Fifty-four naive male Wistar rats of 9 weeks age were classified into GT, ST, or Int, following the same PCA index score described in experiment 1, Fear conditioning boxes. Six identical Panlab test chambers (LE111 model), each of them 26 cm high, 25 cm long, and 25 cm wide were used for pre-exposition, conditioning, and testing. Each chamber was located inside a soundproof module (LE116 model). The walls of the test chambers were made of acrylic white plastic. The bottom of each chamber had stainless steel rods of 2 mm of diameter spaced at 10mm intervals (center to center). The US lasted for about 1s at non-codified 0.5 mA, 50 Hz AC shock in the legs from a constant current generator (LE100-26 model). A loudspeaker located on the top of the chamber at 70 dB of 2.8 kHz distributed in 25 tones lasting 30s each that were used as conditioned stimulus. The bottom of the chambers was located on top of a platform that recorded the movements of the animal. Experimental software PANLAB Start fear was used to calculate the percentage score of general activity in order to detect the ratio of total movement time.
Fear conditioning: Procedure. This phase started a week after the initial procedure in which animals were characterized as ST, GT, or Int. The experimental design was a 3x2 factorial (phenotype as ST, GT, or Int, and exposition to future CS under two conditions; notexposed (NPe) condition: animals not previously exposed to the CS; exposed condition (Pe): animals exposed to the CS prior to conditioning) ST-NPe/ST-Pe, GT-NP/GT-Pe). For Pe animals, each group received 25 occurrences of an independent tone, while the NPe group was kept for an equivalent period of time in the test chambers with no additional stimuli. The evaluation session for LI started with a 300 s period without stimulation. After that, animals were exposed to 25 trials of a 30 s tone (future CS). The inter-trial interval was 30 ± 10 s. Animals in NPe condition were trained for an equivalent period without CS.
Conditioning phase. A single conditioning trial started 30 s after the last tone (or equivalent time period for NPe groups) and consisted of a single pairing of the 30s tone (CS) and an US (electric shock, 1 s, 0,5 mA) at the end of the CS. A 180 s interval separated test from conditioning; during this time, animals underwent trials followed by a single tone for all conditions. General activity was registered during the exposition to the tone (or an equivalent period of time for animals in the NPe condition). Data analysis. As indicated above, ANOVA test and Tukey post-hoc test was used in order to analyze freezing conditioned by groups. Significant level was established at p < .05. showed that during preconditioning phase, animal activity was similar and no statistical differences were found (all ps>0.05). However, during the post-conditioning phase the analysis revealed that in GT Pe group the conditioning was less than the observed in GT NPe group (p = 0.000) and there were no differences between ST-Pe group and ST NPe group (p = 0.54). In the ST conditions there was an effect of Phase, F(1, 26) = 20.33, p = 0.000, and Preexposure, F(1, 26) = 13.04, p = .001 but there was no effect of the interaction Phase x Preexposure (p = 0.74) indicating that for this phenotype rats increased freezing to CS from pre-conditioning to post-conditioning phases but this effect was not modulated by the preexposure. There was a significant difference between preconditioning and post-conditioning phases in ST-Npe group (p = 0.039). Freezing also increased significantly from preconditioning to conditioning phase in ST Pe group (p = 0.018). In the GT conditions, the interaction Phase x Preexposure was significant, F(1, 24) = 4.74, p = 0.04 due to GT NPe rats increased freezing from Pre-conditioinig Phase to Post-conditioning phase (p = 0.002) but GT Pe group displayed a clear LI effect, there was no difference from pre-conditioning phase to conditioning phase (p = 0.519).

Discussion
We explored the role of the PFC, developmental state, incentive salience of stimuli, and processing of unrewarded stimuli in GT vs ST rats. Lesioning the mPFC affected selectively the ST phenotype. The distribution of animals showing ST and GT phenotypes changed with age, this could be related to developmental trajectory of the mPFC among others developmental factors. Finally, attention to previously exposed stimuli was processed differently in GT vs ST rats. Experiment 1 data suggest a different role of the mPFC in GT and ST rats. Even though we were not able to know the profile of an animal before lesion, since it would have affected to post-lesion performance, the comparison with a sham allowed us a general sight of the possible involvement of mPFC in ST and GT profile distribution. Although we do not have evidence that both sham and mPFC lesion animals learn the CS-US relationship equally well, lesion to mPFC selectively affected the ST group, reducing the probability of finding a ST profile and leading to an increase in the number of GT animals. This result could be explained by the data obtained by Haight et al. [37], who observed that c-fos activity increased in neurons of the prelimbic cortex after autoshaping training in both GT and ST groups. This increment in activity in the prelimbic cortex correlated with an increment in the activity of the paraventricular nucleus of the thalamus in GT, but not in ST [38]. Haight et al. [37] suggested that greater activity of mPFC neurons could reduce the incentive salience of stimuli, allowing a better topdown control. However, mPFC lesions yielded a reduction in ST, facilitating a GT response. This data is not consistent with a top-down attribution of mPFC but supports the idea of the PFC modulating the attribution of incentive salience to a stimulus. Such control could be accomplished in conjunction with other subcortical areas linked to a cortico-striatal loop that would converge on the NAc [39,40]. Experiment 2 shows the effects of incrementing the incentive salience of a stimulus by increasing the deprivation of food in juvenile vs. late adolescent rats. Immature animals showed a transfer to the ST endophenotype throughout Phase B. It is possible that an immature mPFC does not allow reducing interference processes when animals have to choose between different responses, resulting in high activity of both lever press and number of magazine entries in Phase B. Furthermore, these animals show top-down actions together with the activities typical of an ST endophenotype. On the contrary, late adolescent animals showed a clear change of strategy, moving from a GT to an ST endophenotype when the incentive salience of the CS was increased. However, when they returned to initial conditions they did not show the same performance (Phase A'). This data indicates different profiles could be effective for classification only in the first sessions due to manipulations to the procedure is sensitive to learning processes.
In rodent models, PFC undergoes a marked change in neural circuit connectivity, gene expression, and functionality [31] that could explain the differences found in juvenile vs. late adolescent rats. Previous studies suggest that ST endophenotype can be the expression of a transitory late developmental stage and the persistence of this phenotype in adulthood may be due to genetic factors [41]. The expression of ST endophenotype over GT has been associated to some aspects of impulsive behavior [42], and also with changes in brain neurotransmitters as dopamine or serotonin. The results of Experiment 2 show that there are also motivational factors that affects the expression of these two endophenotypes.
The last experiment analyzed whether exposure to unrewarded external stimuli could yield different outcome of those stimuli when converted to CS in ST vs GT rats. Experiment 3 analyzed the activity of these two groups in one of the most interesting paradigms measuring attentional processing: latent inhibition. While GT animals showed normal conditioning and latent inhibition, the ST group showed a reduced capacity for latent inhibition. These animals showed a greater conditioning than GT animals, indicating a poor ability to reduce attention to irrelevant stimuli. This data is interesting due to the mPFC ability to decrease attention to any salient cue [25]. Other studies using taste aversion conditioning have shown that lesion to the mPFC increased latent inhibition in animals with a limited exposure to future CS but had no effect in animals with extended training. These results have been interpreted as the mPFC could have a role in coordinating controlled and automatic response [25]. In Experiment 3, ST animals showed a reduced latent inhibition with a limited exposure to future CS that could be explain by a failure of attentional processes (habituation?) to irrelevant stimuli. A mPFC dysfunction has been related to increases in attentional levels and therefore to a lowered latent inhibition. For instance, a predisposition to follow cues regarding drugs in ST animals may be a combination of an excessive attribution of incentive properties to the cues, reduced attentional control, and a propensity for impulsive behavior [11,43]. These results are in line with previous data reveling differences in the neural underpinnings of GT and ST behaviors.
In conclusion, using an established model of Pavlovian conditioned approach behavior that categorize the subjects into different phenotypes depending on their behavior, we have shown that the expression of ST and GT phenotypes is modulated by development, motivational state (o incentive salience of the stimuli) and attentional processes. These findings further our understanding of the role of the prefrontal cortex in the expression of these phenotypes to amplify existing variation in behavioral and physiological responses to conditioned cues. Moreover, the differences in GT and ST rats reported here may have implications on mental health risk factors for disorders (see [44]) such as hyperactivity or substance abuse disorder. Our findings could be interpreted as the ST endophenotype presenting traits associated with vulnerability to disorders related to biogenic amines excess activity in the mPFC.