A microfluidic based biosensor for rapid detection of Salmonella in food products

An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor’s ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor’s selectivity was tested using non-specific binding of E. coli O157 and E. coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E. coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5–3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45–60%.


Introduction
The Center for Disease Control and Prevention (CDC) estimates that around 48 million people in America get sick, with 128,000 hospitalizations and 3,000 deaths due to foodborne diseases annually [1]. Salmonella is ranked first out of the five major pathogens that contribute to domestic foodborne illnesses resulting in hospitalization and death [2]. In 2013, the Economic a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 Colorimetric. For example, Suaifan et al has designed an optical colorimetric biosensor for detection of Staphylococcus aureus and achieved LOD of 7 CFU/ml in pure culture while 40 CFU/ml in food culture [28]. (3) Mass based biosensor. This includes (a) Acoustic wave-based biosensors. For example, a micro-nano-bio acoustic system using magnetic beads to capture cells has detected Salmonella at only 2 cells/μl in 7 hours [29]. (b) Quartz crystal microbalance (QCM) method. For example, a QCM-based platform using ssDNA aptamers has achieved detection of 10 3 CFU/ml of Salmonella within only 1 hour [30]. (c) Cantilever and based biosensor. For example, cantilever biosensor in dynamic-mode without surface functionalization has successfully detected E.coli at 100 cells/ml [31]. Table 1. shows detection of Salmonella using different technologies in various food matrices. Although many of these methods have provided promising research results, in some situations they have relevant drawbacks in terms of detection limit, testing setup, sensitivity and specificity. Impedimetric based biosensor [40], as one kind of the electrochemical biosensors, has shown its promising advantages for detection of foodborne pathogens in terms of detection speed, accuracy, and sensitivity [41]. Such biosensors will have impedance of the electrodes changed in response to captured bacterial cells on electrodes.
In this study, an impedance-based biosensor for simultaneous detection of multiple Salmonella serotypes has been designed, fabricated, characterized, and validated using ready-to-eat turkey (RTE) samples. A detailed study on antibody immobilization which includes antibody concentration, antibody coating time and use of cross-linker has been performed. Detection of Salmonella Typhimurium with various concentrations was utilized using both RTE turkey samples and pure culture samples. The sensor's selectivity using different bacterial cells and capability to differentiate between dead and live cells were also validated. The labeling procedure has been eliminated by immobilization with bioreceptors (in our case antibodies) on the surface of the detection electrode. Impedance change was recorded after selectively capturing the target bacteria by the specific antibody on the surface of the electrodes [42]. An impedance analyzer was used to read the impedance changes.

Biosensor design
The biosensor consists of two detection regions fabricated using SU8 microchannels as follows, Fig 1. Each detection region consists of micro-gaped interdigitated electrode (IDE) arrays fabricated in the same microchannel that incorporates impedance measurement principles to detect the presence/ absence of Salmonella. Each electrode arrays consist of 50 finger pairs with finger length, width, and spacing between the fingers are 90 μm, 10 μm, and 10 μm, respectively. A COMSOL simulation were performed in order to determine the optimum dimension of the interdigitated electrode array. The simulation is shown in Fig 2, the modeling results show that the miniaturized dimensions significantly increase the impedance measurement sensitivity, with the spacing between fingers have greater influence on the strength of the E-field intensity compared with the width of the fingers. The bonding pads are stretched away to the sides of the device and used for impedance measurements. The microchannel in the sensing region has a width and height of 100 μm, 25 μm, respectively. The two detection regions were pre-functionalized by flowing a specific anti-Salmonella antibody serotypes, B or D, to the electrode through independent antibody inlets without causing cross-contamination. All the antibodies were pre-attached with crosslinker. After the microchannel was filled with antibody solutions, the flow was stopped for 1 hour to ensure efficient adhesion of the antibodies to IDE arrays. The Salmonella samples were tested by flowing them through the sample inlet towards the sensing IDE arrays. After the sensing channel was filled with the Salmonella samples, the flow was stopped for 30 minutes to facilitate the contact and binding between Salmonella antigens and the corresponding Salmonella antibodies. It is noted that each device was used for one time only to eliminate the possibility of sample contamination.

Biosensor fabrication
The biosensor was fabricated on a 2×1.5 inch 2 glass substrate using surface micromachining in following steps: (1) The glass slide was first cleaned using Piranha solution (hydrogen peroxide and sulfuric acid with a ratio of 1:3) for 4 minutes. (2) A 4 μm layer of SU-8 2005 (Microchem) was spin-coated on the surface of the glass slide following by pre-bake, UV light exposure and The first one is used to cover the microchannel, while the second layer serves as top cover with fluidic connectors. The first layer was treated with oxygen plasma to change its surface to be more hydrophilic before it was aligned and bonded to the glass substrate. Then the bonded PDMS layer and glass slide were baked on a hotplate at 65˚C for 5 minutes to ensure the bonding between the PDMS and the glass substrate is secured. The second PDMS slab with inlet and outlet fluidic connectors were then bonded to the first layer of PDMS using oxygen plasma. Epoxy glue was used to seal the fluidic connectors further to improve the device reliability. Scanning electron micrographs (SEMs) of the fabricated devices are shown in

Salmonella typhimurium and culture preparation
Ready-to-eat turkey breast (RTE) turkey breast was purchased from a grocery store and stored at 4˚C until use. Each 325 g RTE turkey breast was weighted and placed in a sterile bag, 2925 ml buffered peptone water was poured into the bag, and the bag was shaken for one minute.
The supernatant was then filtered through a 100 μm and then a 20 μm cell strainer (pluriSelect Life Science, Leipzig, Germany) to remove big debris which may block the biosensor microchannels. The filtered rinse was used freshly to dilute Salmonella cells or aliquoted and frozen at -20˚C until used. The effect from solution has been minimized because all biological samples were prepared with buffered peptone water. An avirulent Salmonella enterica Typhimurium strain (ΔsipB, Cmr) was then used to spike RTE turkey breast or pure culture. An overnight culture (37˚C, 200 rpm, in Luria-Bertani broth) of S. enterica Typhimurium was harvested by centrifugation at 4000 rpm 10 minutes and washing with sterile distilled water 3 times and then suspended in 25% sterile glycerol. The cell suspension was aliquoted and frozen at -80˚C until used. At the same time, one aliquot was serially diluted and plated on Luria-Bertani agar plates to determine the cell concentration. The cell concentration was determined to be 2 × 10 9 CFU/ml. Before the test, one aliquot Salmonella suspension and several aliquots of RTE turkey rinse were thawed on ice. The Salmonella suspension was then diluted with the filtered RTE turkey rinse to desired concentrations. It is noted that spiked turkey samples do not mimic infections. The focus of the present study is food-safety testing, not disease diagnosis.
Our protocol did not involve grinding meat samples. Filtration through a cell strainer is actually very simple method to remove debris, which only takes less than 15 minutes. Enrichment culture was evaluated because the current food safety guideline requires considerably a large amount/volume of sample (325 g RTE-turkey sample in 2925 ml buffered peptone water). We are comparing our device with the current popular methods. Compared with PCR, our method does not need the DNA extraction and PCR amplification which can be a difficult to animal farms or food processing plants. Compared with culturing method, our method can save bacterial growing time and the following bacterial identification time which are around 1-2 days. The sample preparing (I mean sampling from food) for all methods including our method are all the same. There is a stomaching step involved with the same purpose of grinding. But, the step is only several minutes. For other methods, whether or not having an enrichment, it really depends on bacterial concentrations in the contaminated food. For real application, a test can be conducted before enrichment and a heavy contamination, though it is rare, can be identified in this step. Another test can be conducted after an enrichment to identify a light contamination.

Antibody preparation
Rabbit anti-Salmonella O antiserum poly B, D, and E (Becton, Dickinson and Company, Franklin Lakes, NJ) were used as Salmonella antibodies. The crosslinker, sulfosuccinimidyl 6-[3-(2-pyridyldithio) propionamido] hexanoate (sulfo-LC-SPDP) (Fisher Scientific, Hampton, NH), was used for antibody immobilization. Briefly, for each test, 16 μl of each antiserum was diluted with 288 μl filtered chicken rinse, mixed with 300 μl sulfo-SPDP (20 mM water solution), and then incubated at room temperature for 1 hour. To reduce the disulfide bond of the thiolated antibody, 200 μl DTT (0.1 M sodium acetate buffer, 0.1 M NaCl, pH 4.5) (Fisher Scientific, Hampton, NH) was then added into the tube to react for 30 minutes at room temperature before the antiserum mixture was loaded into the biosensor. The antiserum-crosslinker mixtures were then diluted with distilled water in the ratio of 1:100 or 1:50 to form 1× antibody concentration and 2× antibody concentration, respectively.

Antibody crosslinker
A new immobilization method using cross-linker was used to exploit natural and direct covalent bond formation between gold electrode and a thiolated antibody. The cross-linker we used is Sulfo-LC-SPDP, it is a thiolation reagent that introduce available sulfhydryl groups to an antibody. A monolayer coating of the thiolated antibody onto the gold surface can be easily achieved because the reaction potential between the gold and sulfur is very strong, which achieved 50% in signal response [43,44]. In our experiments, all the antibodies were prepared with crosslinker.

Antibody immobilization
The diluted mixtures of antibody with cross-linker were delivered to the sensor through inlets using syringe pumps at a constant flow rate for 15 minutes. Subsequently, the sensing region was then functionalized with two types of antibodies, with one for each sensing region without causing any cross contamination. The flow was stopped for 1.5 hours to allow the antibodies to get firmly immobilized on the gold IDE arrays to achieve a good and specific binding between the antibodies and the gold IDE arrays. A washing step by pumping DI water into the channel was then performed for 30 minutes to remove excess antibodies and other waste materials. Impedance was then measured by an impedance analyzer. The flow rate for all fluids was set to be 2 μL/min to prevent fluids from flowing in random fashion. To confirm antibody adhesion to the IDE arrays, we have measured the impedance of the electrode before antibody coating, and after antibody coating. The impedance change was significant (See Fig 5). The antibody used in all experiment was prepared with DI water. The measured impedance value before antibody coating was less than 0.50 MΩ, while after antibody coating, the impedance value was increased to a value above 2.1MΩ.

Antigen capturing
The prepared Salmonella sample were then introduced into the channel through the sample inlet by a syringe pump at a constant flow rate. After the channel was filled with sample, the flow was stopped for 30 minutes to allow the binding between the Salmonella antigens (type B) and Salmonella antibodies (type B). Then, the channel was washed again for 30 minutes to remove the unbound or weakly bound antigens and other unwanted particles. The impedance was then measured. The impedance change was calculated by subtracting the measured impedance after antibody coating from the measured impedance after antigen binded with antibody. The impedance difference represents the impedance value of antigens alone. All samples were filtered to remove large food particulates before they were delivered into the sensor. It is noted that the chips are one-time use for all the experiments. The chips are suitable for repeating use with appropriate cleaning process. But the food industry prefers the sensor to be disposable to eliminate the possibility of cross-contamination. In the future when the sensor is manufactured in industry, antibody coating would be a part of the sensor manufacture process. Based on the request from the food industry, the reusability of the sensor was not included in this paper.
Data acquisition. Agilent 4294A impedance analyzer was used to record the impedance. A sine wave of 500mV peak voltage was applied across the terminals of the IDM arrays and the corresponding impedance values were measured for frequencies between 100 Hz and 10MHz.

Antibody immobilization study
There are potentially three factors during antibody immobilization that would affect the overall performance of the biosensor: antibody coating time, antibody concentration and use of cross-linker. Fig 6 and Fig 7 show the study on antibody immobilization based on pure culture spiked Salmonella of 500 and 1000 Cells/ml. The antibodies were injected from the antibody inlets. Once the detection channels were filled, the flow was stopped for 0.5, 1, 1.5 and 3 hours before the channel/ electrodes are washed to remove the access components. Four different immobilization time ranged from 0.5 to 3 hours were chosen for the study while the antibody concentrations are at single (1X) and double (2X) concentration. It was observed that at 0.5 hours, there is a small change in impedance, while 1-hour coating time seems to provide a higher impedance value which indicates better results from antibody immobilization. The results from 1.5 and 3 hours gave a similar impedance change but higher than the result from 1-hour, which means the binding of antibody to IDE arrays have reached equilibrium. Therefore, 1.5 hours was demonstrated as the best immobilization time for achieving an optimum coating. To study the antibody concentration effect, we tested the biosensor with single and doubled antibody concentration, it was found that the concentration of the antibody doesn't have a significant effect on impedance response due to saturation of binding sites on IDE arrays to allow absorption of antibodies. For all the experiments, the use of crosslinker has improved the signal response by 50%. It is necessary to have a stable and firm binding of the antibody to electrode for a better sensor stability. was then delivered to the sensor. The results demonstrated a high impedance difference of the electrode with Salmonella antibody serotype B while the other electrode where antibody D was coated had a weak signal. The detection limit of the sensor was found to be 300 Cells/ml. For all concentrations, impedance decreases as a function of frequency. Fig 8(C) shows the Impedance change as a function of Salmonella concentration at 1 kHz. We found the variation of impedance change is directly proportional (linear) to Salmonella concentrations. The linear correlation coefficient is 0.965 and the sensitivity (Slope) is 0.144 kO per one Salmonella cell. All the sample concentrations were pre-confirmed by the culture method, so the number of bacterial cell colonies is known before being delivered to the sensor for testing. The device cannot quantify the number of detected bacterial cells, but it informs the presence or absence of bacterial cells.

Simultaneous detection of two types of bacterial cells
In the first set of experiments (Fig 9), two detection regions were coated with the antibody for Salmonella serotype B and Salmonella serotype D, respectively without causing cross-contamination. Then mixture of Salmonella serotype B (300 Cells/ml) and Salmonella serotype D (300 Cells/ml) was delivered to the sensor (Fig 9A). A mixture of Salmonella serotype B (630 Cells/ml) and Salmonella serotype D (290 Cells/ml) was delivered to the sensor (Fig 9B). The results demonstrate Ready-to-eat turkey culture with crosslinker. The sensor was first functionalized with Salmonella antibody type B and D with crosslinker. Baseline impedance after antibody coating was calculated. Salmonella serotype B with concentrations of 1000, 500 and 300 Cells/ml spiked with RTE turkey sample was then delivered to the sensor for detection. Then impedance after antibody was subtracted from the impedance measured after we delivered the antigen to get the impedance change. The limit of detection was found to be 300 Cells/ml. (c) Impedance change as a function of Salmonella concentration at 1 kHz. Impedance change is directly proportional (linear) to Salmonella concentrations. The linear correlation coefficient is 0.965 and the sensitivity (Slope) is 0.144 kO per one Salmonella cell. AB stands for antibody. AG stands for antigen.
https://doi.org/10.1371/journal.pone.0216873.g008 Cells/ml, 300 Cells/ml and (b) 300 Cells/ml, 300 Cells/ml. AB stands for antibody and AG stands for antigen. Impedance after antibody coating was recorded and subtracted from the impedance after we delivered the antigen to get the impedance change. https://doi.org/10.1371/journal.pone.0216873.g009 Detection of Salmonella in food products that the impedance value was doubled when the Salmonella B concentration was doubled, which demonstrated that the sensor can selectively and simultaneously detect multiple pathogens.
In the second set of experiments, (a) the two detection regions were coated with antibody for Salmonella serotype B, then a mixture of Salmonella serotype B (600 Cells/ml) and E.coli O157 (600 Cells/ml) was delivered to the sensor as shown in Fig 10A. (b) Only Salmonella serotype B (600 Cells/ml) was delivered to the sensor as shown in Fig 10B. The impedance change values in experiments from (a) and (b) were similar. The mixture of Salmonella with E. coli O157 did not increase the impedance value. This indicates that E. coli O157 contamination did not have any effect on the detection of Salmonella, and proves the detection is selective. (c) Only E.coli O157 (590 Cells/ml) was delivered to the sensor, the impedance change is low ( Fig  10C). The results demonstrated the sensor's capability to selectively detect target bacterial cell based on the specific type of antibody coating. The nontarget bacterial cell (E. coli O157) would not bind to the antibodies on electrodes thus would be washed away during the following washing step. The results demonstrated the sensor's capability to selectively detect target bacterial cell based on the use of antibody.

Sensor's Selectivity Using Salmonella and E. coli O157
Salmonella and E.coli O157 were used to test the sensor's selectivity (Fig 11). In the first set experiments, the detection regions were coated with Salmonella antibody type B. The RTE turkey samples were spiked with only Salmonella type B at different concentrations were introduced into the sensors individually without mixing. In the second set experiments, the detection electrodes were coated with the antibody for Salmonella serotype B. Then only E.coli O157 at different concentrations were introduced into the sensors without mixing. The result from these experiments demonstrated that the sensor with E.coli antigens had a weak signal response while the other sensor with Salmonella serotype B antigens has a strong signal response. This demonstrates the device ability to selectively detect Salmonella serotype B.

Live and dead Salmonella cells differentiation
The sensor was tested with live and dead Salmonella cells to demonstrate its ability to differentiate between them. The Salmonella cells were killed by brief exposure to high temperature which resembles real-life event. In each experiment, two sensing regions from single sensor were coated with the same anti-Salmonella serotype B, while dead and live bacteria were pumped into the sensor through the antigen inlet. Fig 12 indicates that the signal response for the dead bacteria was very low, which was expected because the membrane of Salmonella antigen was damaged and thus lost the function to bind with Salmonella antibody.
We have plotted the lowest concentration of Salmonella (300 cells/ml) in ready-to-eat turkey samples, dead Salmonella cells (1000 cells/ml), non-specific binding E. coli O157 (1000 cells/ml) and E.coli DH5 Alpha (1000 cells/ml) in Fig 13 to show clearly the difference in impedance values. The figure demonstrates that the lowest concentration of Salmonella can be differentiated from high concentration of dead cells and non-specific binding E. coli O157 and E. coli DH5 Alpha.

Testing time
Prior to testing, Salmonella antibodies was mixed separately with the cross-linker (Sulfo-LC-SPDP) and was injected into the device via the antibody inlet for 15 minutes. The flow was stopped for 1.5 hours to allow the antibodies-crosslinker to get firmly immobilized on the gold IDE arrays to achieve a good and specific binding between the antibodies and the gold IDE arrays. The channel was then washed for 30 minutes with DI water to remove the unbounded antibodies and other materials, and the impedance of each IDE array was measured. Next, the bacterial testing sample was introduced into the biosensor by syringe pump via the sample inlet. The flow was stopped for 30 minutes to allow the binding between the Salmonella antigens and Salmonella antibodies. After cleaning the channel with water for 30 minutes, the impedance was measured again, where the impedance change indicated the presence/absence of bacterial cells. We have chosen 30 minutes washing step to ensure that only antibody-antigens are present on the detection electrodes. The other 30 minutes for the antigens to bind to antibody, is not optimum time. We have chosen it in order to maximize the number of antibody-antigens binding. We are currently experimenting to determine the minimum time needed to wait before measuring the antibody-antigens impedance. We expect the detection time to drop much lower. Therefore, the overall testing time is 3 hours and 15 minutes which include the antibody coating time, antigens binding time and the multiple washing step time. It is noted that the biosensor will be used as a disposable device to eliminate the possibility of contamination, per the recommendation of food processing companies including major poultry industry such as Tyson, Cargill, and Pilgrim's. The device must be pre-coated with antibody cross linker mixture prior to sale. Therefore, the testing time that will be counted is the testing and washing step time, which is 1 hour.

Electrical Equivalent Circuit and simulation
An equivalent circuit of the sensor was created to study the impedance response and electrical properties. Fig 14(A). represents the equivalent circuit of the impedance biosensor which Detection of Salmonella in food products consists of two double layer capacitances (C dl ) connected in series with the solution resistance (R sol ) and parallel to another capacitance (C cell ). The electrode pair with each one has an area of A and spacing of G, were placed in parallel. When an AC voltage (v) was applied to the electrode pair, an electrical current flow (i) and a solution resistance R Sol would be generated as: R Sol represents resistance of the testing solution between the electrodes where ρ Sol is the solution resistivity. R Sol is proportional to the concentration of the pathogen in the tested sample. A thin layer of charged particles was formed on the surface of the electrodes, which would generate capacitance C dl . In the equivalent circuit, C dl and R Sol are connected in parallel with the direct capacitive coupling between the electrodes (C cell ) which depends on the solution dielectric property and the geometry of the electrodes [45]. The parasitic resistors (R pa ) represents resistance from electrical connections and wires of the circuit which is small and thus can be neglected [45,46]. R Sol and the two capacitors C dl impedance together will determine the overall impedance, which is expressed as: EIS spectrum analyzer software was used to simulate the response of the equivalent circuit. Fig 14(B) shows the results from experiment and simulation (Bode plot) for the electrical equivalent circuit. The simulation value of C dl was calculated to be around 20 nF and R Sol was calculated to be from 30 kΩ to 120 kΩ depending on the concentration of the bacteria. At low frequencies region (below 100 Hz) the impedance response is dominated by capacitive impedance C dl . At medium frequency region (100 Hz − 50 kHz) the response is due to both resistive and capacitive components while R Sol is the dominant in this region [46,47]. At high frequency (above 50 kHz), the impedance becomes pure resistive value [47]. Therefore, the impedance response at high frequencies merely depends on the resistive component of the solution, which means the effect from bacterial cells is insignificant.
The USDA/FDA set a zero-tolerance requirement for RTE poultry products. The testing method must meet the AOAC standard for certification, i.e., 1 cell/325 gr of product. Therefore, a short enrichment step is still required. However, due to the detection limit of our biosensor 300 cell/ml, the total detection time that includes an enrichment will still be less than 24 hours, which is faster than traditional food screen methods like ELISA and PCR. In experiment, by applying the USDA's Laboratory Guidebook which suggests that the enrichment culture is done by inoculating 325 gr RTE poultry into 975 ml BPW at 1:4 dilution. For example, when 10 Salmonella cells inoculated on 325 gr RTE Turkey sample, it took approximately 8 hours to reach 400 cells/ml, which can be detected with our biosensing device. For raw poultry products, rather than creating a zero-tolerance standard the USDA maintains a minimum number of samples that must be collected from broiler carcasses [48]. The initial level of bacteria in raw poultry is high, but it is lowered through the processing line by immersing the carcasses in generally recognized as safe (GRAS) substances such as peroxyacetic acid at multiple sites (e.g., Pre-Chill, Chill 1, Chill 2, and Chill 3). At the end of the processing line the remaining bacterial cells must meet the minimum requirement. For example, in a turkey plant, a maximum of 4 samples may test positive for Salmonella, in a row of 52 samples [49]. Therefore, the device can be used in the poultry slaughtering plants in the process line at multiple locations to obtain the results in < 1 hr, without the need for a sample enrichment step.
The sensor will be used to determine if the tested poultry sample is contaminated with Salmonella, i.e., the device will tell us if the sample is positive or negative to Salmonella contamination. It cannot be used to quantify the number of pathogens in the sample. Once the sample is confirmed positive, then other standard techniques can be used to confirm the results and quantify the bacterial cell number.

Conclusion
This paper has presented design and fabrication of an impedance-based MEMS biosensor for simultaneous detection of two types of Salmonella serogroup (type B, and D) detection. The biosensor enables rapid and quantitative detection of Salmonella in food source. The device consists of two microfluidic channels with each has an IDE array for bacterial cells detection. This design makes the sensor suitable for simultaneous detections of two different bacterial cells, independently without causing any cross contamination. The results demonstrate that the impedance difference increases when the concentration of target bacteria increases, with the limit of detection found to be 300 Cells/ml. The lowest measured concentration of Salmonella can be differentiated from high concentration of dead Salmonella cells, and high concentration of non-specific binding E. coli O157 and E. coli DH5 Alpha. In addition, the device can selectively detect bacterial cells based on the matching antibody. A detail study of antibody immobilization conditions that includes antibody coating time, antibody concentration, and use of cross-linker has been presented. It is shown that the sensitivity has been improved by 45-60% with the use of Sulfo-LC-SPDP as cross-linker. The binding of Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after bonding sites on electrode are saturated. 1.5 hours was found to be the optimal antibody coating time. The sensor has also shown its specificity among different Salmonella serotypes, selectivity on different types of bacterial cells, and capability to distinguish between dead and live cells with the total detection time of 1 hour.