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Courtney Davis-Vogel, Brandon Van Allen, John L. Van Hemert, Amit Sethi, Mark E. Nelson, Dipali G. Sashital
Identification and comparison of key RNA interference machinery from western corn rootworm, fall armyworm, and southern green stink bug
www.plosone.org
www.plosone.org
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(in)Tj
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(11)Tj
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(,)Tj
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(12)Tj
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4.507 0 Td
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(13)Tj
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1.8369 0 Td
(2125)Tj
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(duplexes)Tj
3.7133 0 Td
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3.2655 0 Td
(\(DCR-2\))Tj
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([)Tj
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(14)Tj
0 g
(].)Tj
-10.7092 -1.2983 Td
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(duplex)Tj
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(either)Tj
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(pathway)Tj
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(is)Tj
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(an)Tj
1.2075 0 Td
(argonaute)Tj
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(tein)Tj
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(family)Tj
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(member,)Tj
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(usually)Tj
3.0217 0 Td
(assisted)Tj
3.2485 0 Td
(by)Tj
1.1848 0 Td
(a)Tj
0.652 0 Td
(dsRBP,)Tj
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(forming)Tj
3.4866 0 Td
(an)Tj
1.2019 0 Td
(RNA-induced)Tj
5.862 0 Td
(silencing)Tj
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(complex)Tj
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1.7801 0 Td
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(ssRNA)Tj
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(targets,)Tj
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(1)Tj
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(\(AGO1\))Tj
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(and)Tj
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(the)Tj
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(dsRBP)Tj
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(\(LOQS\))Tj
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(to)Tj
1.0261 0 Td
(form)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(miRNA)Tj
3.3505 0 Td
(RISC)Tj
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(\(miRISC\))Tj
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([)Tj
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(15)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
3.5433 0 Td
(In)Tj
1.1111 0 Td
(an)Tj
1.2019 0 Td
(analogous)Tj
4.252 0 Td
(process,)Tj
3.4072 0 Td
(a)Tj
0.6576 0 Td
(guide)Tj
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(siRNA)Tj
-34.4634 -1.3039 Td
(is)Tj
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(preferentially)Tj
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(loaded)Tj
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(into)Tj
1.8368 0 Td
(Argonaute)Tj
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(2)Tj
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(\(AGO2\))Tj
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(with)Tj
2.0012 0 Td
(the)Tj
1.4683 0 Td
(assistance)Tj
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(of)Tj
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(DCR-2)Tj
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(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(dsRBP)Tj
-34.6845 -1.2983 Td
(R2D2)Tj
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(to)Tj
1.0262 0 Td
(form)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(siRNA)Tj
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(RISC)Tj
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(\(siRISC\))Tj
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0.83 0.64 0.02 0 k
(20)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(].)Tj
3.549 0 Td
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1.7801 0 Td
(final)Tj
2.0013 0 Td
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(RISCs)Tj
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(miRISC)Tj
3.4072 0 Td
(will)Tj
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(bind)Tj
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(nt)Tj
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(segmentstypically)Tj
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(within)Tj
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-35.5575 -1.3039 Td
(3')Tj
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(untranslated)Tj
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(transcriptscontaining)Tj
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1.0261 0 Td
(the)Tj
1.4684 0 Td
(miRNA)Tj
3.3505 0 Td
(seed)Tj
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(28)Tj
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(the)Tj
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(miRNA)Tj
3.3505 0 Td
(5)Tj
1.0885 0 Td
(end)Tj
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(23)Tj
0 g
(]\).)Tj
2.4151 0 Td
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1.7859 0 Td
(remainder)Tj
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(sequence)Tj
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(contain)Tj
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(imperfect)Tj
4.0536 0 Td
(complementarity,)Tj
7.2963 0 Td
(resulting)Tj
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(one)Tj
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(miRNA)Tj
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(having)Tj
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(the)Tj
1.4741 0 Td
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(many)Tj
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(transcripts)Tj
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5.2101 0 Td
(repression.)Tj
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1.7859 0 Td
(active)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 377.4047 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Study)Tj
2.7425 0 Td
(of)Tj
1.0417 0 Td
(RNAi)Tj
2.5158 0 Td
(gene)Tj
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0.978 0 Td
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2.863 0 Td
(FAW,)Tj
2.7 0 Td
(and)Tj
1.8567 0 Td
(SGSB)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
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0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
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(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
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(ne.02031)Tj
4.089 0 Td
(60)Tj
0 g
2.0906 0 Td
(September)Tj
5.0386 0 Td
(5,)Tj
1.0418 0 Td
(2018)Tj
32.8254 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(26)Tj
/F10 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(\(s\),)Tj
1.4811 0 Td
(and)Tj
1.5874 0 Td
(the)Tj
1.3677 0 Td
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(may)Tj
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(not)Tj
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(able)Tj
1.7362 0 Td
(to)Tj
0.9426 0 Td
(provide)Tj
-14.5135 -1.3748 Td
(materials)Tj
3.6071 0 Td
(including)Tj
3.5858 0 Td
(third)Tj
1.9276 0 Td
(party)Tj
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(elements)Tj
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(the)Tj
1.3677 0 Td
(requestor)Tj
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(certain)Tj
2.7142 0 Td
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4.337 0 Td
(restriction)Tj
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2.5087 0 Td
(such)Tj
2.0126 0 Td
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(directly)Tj
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1.5874 0 Td
(authors)Tj
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3.111 0 Td
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(\))Tj
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(the)Tj
1.3677 0 Td
(requester)Tj
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(use,)Tj
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1.9205 0 Td
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2.0977 0 Td
(proprietary)Tj
4.2732 0 Td
(materials.)Tj
3.8339 0 Td
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-14.804 -1.3749 Td
(any)Tj
1.5378 0 Td
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2.0055 0 Td
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-11.0055 -2.126 Td
(Funding:)Tj
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0.7583 0 Td
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1.5874 0 Td
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(JVH,)Tj
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2.2606 0 Td
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1.063 0 Td
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1.063 0 Td
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3.4157 0 Td
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1.9559 0 Td
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(and)Tj
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(publish,)Tj
3.1465 0 Td
(or)Tj
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(preparation)Tj
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q
1 j
1 J
0 w
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1 TL
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q
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BT
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3.6 0 Td
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(each)Tj
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(other,)Tj
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1.0262 0 Td
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4.2179 0 Td
(previously)Tj
4.3483 0 Td
(reported)Tj
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(insect)Tj
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(and)Tj
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2.2054 0 Td
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4.5807 0 Td
[()75(\n)-9()14()4( )10()12()12()12()]TJ
4.1896 0 Td
[()7()9()11()16()6()2()12()]TJ
/F7 1 Tf
4.4164 0 Td
(\()Tj
/F12 1 Tf
[()97()7()]TJ
/F7 1 Tf
(\),)Tj
/F12 1 Tf
2.5738 0 Td
[()-2( )11()]TJ
-34.5654 -1.2983 Td
[()12()]TJ
1.593 0 Td
[()6( )11(\n)-9()]TJ
/F7 1 Tf
2.092 0 Td
(\()Tj
/F12 1 Tf
[()17()6( )]TJ
/F7 1 Tf
(\),)Tj
2.9366 0 Td
(and)Tj
/F12 1 Tf
1.7292 0 Td
[()30()-9()-13(\n)-19()10()6( )7()10()10()10()6( )11()]TJ
5.8847 0 Td
[()11()-4()10()12()]TJ
/F7 1 Tf
2.5965 0 Td
(\()Tj
/F12 1 Tf
[()30()11()]TJ
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(\))Tj
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5.1023 0 Td
[( )31( )11()6( )]TJ
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[()-3()12()11()4()2()]TJ
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(\()Tj
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[( )31()]TJ
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(\),)Tj
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[()-4()6()]TJ
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(three)Tj
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(the)Tj
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(extent)Tj
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(possible,)Tj
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(the)Tj
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(the)Tj
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0.83 0.64 0.02 0 k
1.8425 0 Td
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1.0318 0 Td
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1.1736 0 Td
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(.)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 372.9826 cm
BT
/F14 1 Tf
1 TL
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Q
q
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(exception)Tj
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[()17()6( )]TJ
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(\(e-\)values)Tj
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