New polybranchiaspiform fishes (Agnatha: Galeaspida) from the Middle Palaeozoic of China and their ecomorphological implications

The 438–370-million-year-old galeaspids, diversified armoured jawless vertebrates (‘ostracoderms’) from China and northern Vietnam, were assumed to have a benthic feeding habit in a coastal, marine environment. Here, we describe two new genera of galeaspid fishes, Platylomaspis gen. nov. and Nanningaspis gen. nov. from the Middle Palaeozoic of China. The two new forms are characterized by a rostral process and strikingly broad ventral rim, and clustered with Gumuaspis to form a new family, Gumuaspidae, which represents the most primitive clade of Polybranchiaspiformes. They extend the earliest occurrence of Polybranchiaspiformes backward about 19 million years, and expand its geographical distribution from southern China and northern Vietnam to the Tarim Basin, northwestern China. The new taxa exhibit many morphological convergences with modern rays, and might specify a new kind of lifestyle of galeaspids, the half burrowing habit. Probably benefiting from the new lifestyle, the Gumuaspidae has become the longest lasting galeaspid family. The new findings demonstrated that the demersal galeaspids had developed three different kinds of lifestyles: semi-infaunal benthic (half buried), epibenthic, and suprabenthic (nektonic) habits to accommodate to differentiated ecological niches, and reached the peak of their diversity by the Pragian of the Early Devonian.


Introduction
The 438-370-million-year-old galeaspids are a diversified but endemic group of armoured jawless vertebrates ('ostracoderms') occurring only in China and northern Vietnam [1][2][3][4][5]. Although galeaspids have no biting jaws, they are regarded as an important stem-group of gnathostomes that provides the crucial fossil evidence for the culmination of stepwise anatomical changes towards crown gnathostomes [6][7][8]. For example, galeaspids have a diagnostic large median dorsal opening for the common nostril of paired separated nasal sacs, which PLOS  reflects an intermediate condition for the developmental establishment of complete diplorhiny and jaws in jawed vertebrates [1,2]. For a long time, galeaspids were simply assumed to be benthic jawless fishes in a coastal, marine environment because they bear a strongly flattened head-shield, dorsally-set eyes and nostril, and a ventral mouth [3]. However, a streamlined galeaspid Rhegmaspis, which was recently known from the Lower Devonian of Yunnan, displays adaptations for a suprabenthic or nektonic lifestyle probably with more active feeding habit among galeaspids [9]. Here, we further describe two new genera of polybranchiaspiform galeaspids, Platylomaspis gen. nov. from the Silurian of the Tarim Basin, Xinjiang, and Nanningaspis gen. nov. from the Devonian of Nanning, Guangxi. The two new polybranchiaspiforms exhibit a rostral process and strikingly broad ventral rim, probably denoting a new lifestyle of galeaspids.

Materials
The

Methods
All the materials were prepared mechanically using a vibro tool with a tungsten-carbide bit or a needle, and some specimens were reversed on latex casts. All specimens were measured with a digital vernier calliper, photographed with a NIKON D3X camera and studied under optical zoom. The phylogenetic character data entry and formatting were performed in Mesquite (version 3.31) [41]. The phylogenetic analysis was conducted using PAUP 4.0a, parsimony analysis package [42].

Nomenclatural acts
The electronic edition of this article conforms to the requirements of the amended International Code of Zoological Nomenclature, and hence the new names contained herein are available under that Code from the electronic edition of this article. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The ZooBank LSIDs (Life Science Identifiers) can be resolved and the associated information viewed through any standard web browser by appending the LSID to the prefix "http://zoobank.org/". The LSID for this publication is: urn:lsid:zoobank.org:pub: DEE5A348-D73F-454E-B627 -040D3B7ADE1C. The electronic edition of this work was published in a journal with an ISSN, and has been archived and is available from the following digital repositories: PubMed Central, LOCKSS.

Remarks.
A new family Gumuaspidae fam. nov. is erected for polybranchiaspid-like galeaspids including Gumuaspis, Platylomaspis, Nanningaspis and Pseudolaxaspis, which bear a rodlike rostral process, but lack laterally projecting corners. The phylogenetic analysis of Galeaspida (see below) shows that the four genera are clustered together to form a monophyletic group, which represents the most primitive clade of Polybranchiaspiformes.
The type genus Gumuaspis was found from the Posongchong Formation (Early Devonian, Pragian) in Wenshan County, Yunnan, China. The new genus Pseudolaxaspis gen. nov. is erected for 'Laxaspis rostrata' from the Xishancun Formation in Qujing, Yunnan. Pseudolaxaspis was originally referred to Laxaspis as it resembles the type species of Laxaspis, L. qujingensis, in many respects such as the shape and position of the median dorsal opening and orbital opening, the ornamentation of the head-shield, and the end of sensory canals. However, it shows evidently a long rostral process ( [43], pl. IV-1), by which 'L. rostrata' is more suggestive of Gumuaspis rostrata than Laxaspis qujingensis (Fig 2A and 2B) [39]. However, if we assign 'L. rostrata' to Gumuaspis, a problem of homonym will be produced since the type species of Gumuaspis is named as G. rostrata. Zhu and Gai suggested an alternative solution to erect a new genus for 'L. rostrata' [4]. Here, we assign a new genus Pseudolaxaspis for 'L. rostrata'. Platylomaspis gen. nov., and Nanningaspis gen. nov. are two new forms of Gumuaspidae from the Silurian of the Tarim Basin, Xinjiang, and the Devonian of Nanning, Guangxi respectively.
Type species. Pseudolaxaspis rostrata (Liu, 1975) Diagnosis (emended). Medium-sized head-shield with a rod-like rostral process; median dorsal opening near circular in outline; orbital opening just behind the level of the posterior margin of the median dorsal opening; end of sensory canal showing a multilateral loop with radial branches; oral fenestra triangular in shape; narrow ventral rim; ornamentation composed of snowflake-like tubercles.
Pseudolaxaspis rostrata (Liu, 1975 Diagnosis. Medium-sized head-shield, 70-80 mm in length (rostral process excluded), and 60-70 mm in width; rostral process rod-like; inner corner broad leaf-shaped; lateral margin of head-shield serrated; ventral rim of head-shield remarkably broad, 18-20 mm in width on each side, approximately one-third the width of the entire head-shield; ventral rim embracing a large pear-shaped oralobranchial fenestra; anterior oral fenestra small, and subtriangular in shape; posterior branchial fenestra large and near circular in shape; ventral rim of either side showing six successive notches for branchial openings; ornamentation composed of coarsegranular tubercles. Diagnosis. As for genus (monotypic).  Diagnosis. Large-sized head-shield about 225-235 mm in length, and 165-175 mm in width; rostral process rod-like; median dorsal openings and sensory canal system unclear; orbital opening small, and probably located on the dorsolateral margin of head-shield; lateral margin of the head-shield smooth; ventral rim of head-shield remarkably broad, 25-40 mm in width from caudally to rostrally, approximately one-fourth the width of the entire head-shield; ornamentation composed of coarse-granular tubercles.

Descriptions
Platylomaspis serratus. It is a moderate sized polybranchiaspiform. The pear-shaped head-shield tapers rostrally into a long rostral process (ro, Fig 3A and 3B). The length of the head-shield is 70-80 mm (rostral process excluded), the maximum width is about 60-70 mm, and the median length is about 55-60 mm. The maximum width is at the middle part of headshield, smaller than its length. The rostral process, although incomplete in both specimens, tapers anteriorly as shown by the preserved part. The preserved rostral process is 13.5 mm in the holotype, 16.4 mm in the paratype.
The paratype (Fig 3A) is an incomplete dorsal head-shield with the largest preserved length of 78.3 mm, and the largest preserved width of 45.7 mm. The paratype shows a small domeshaped depression in the center and a broad flatten marginal band. The roof of the domeshaped depression occupies the main part of the head-shield, but the median dorsal opening, orbital opening and sensory line system are not preserved on it. The space in the dome-shaped depression encloses the oralobranchial chamber (ob.c, Fig 3A). The broad flat marginal band indicates that Platylomaspis serratus has a broad the ventral rim. There are 5-6 serrations every 10 mm along the lateral margin of the head-shield (sl.b, Fig 3A).
The holotype is a nearly complete ventral head-shield (Fig 3B) with the largest preserved length of 65.9 mm, and the largest preserved width of 60.8 mm. The ventral rim on each side is New Silurian and Devonian jawless fishes very broad, about 20.0 mm in width, which is approximately one-fourth the width of the entire head-shield. Normally, the ventral rim only accounts for one-tenth of the entire head-shield in most member of Polybranchiaspiformes (e.g. Polybranchiaspis, Pentathyraspis). Therefore, the exceptional broad ventral rim can be regarded as a diagnostic character of Platylomaspis. An incomplete cornual process with the largest preserved length of 8.7 mm is preserved in the left side of the holotype. The cornual process is broad leaf-shaped, and oriented caudally. Zhu (1992) suggested that the true "corner" probably have secondary lost in the Hanyangaspidae and Polybranchiaspidae, and their developed leaf-shaped cornual process was homologous with the inner corner of Dayongaspis and eugaleaspids [44]. The shape of head-shield and cornual process of Platylomaspis are very similar to that of Polybranchiaspiformes. Therefore, the cornual process of Platylomaspis represents the inner corner, and its true corner is absent (ic, Fig 3B).
The ventral rims embrace a large pear-shaped fenestra for the oralo-branchial fenestra, through which the oralo-branchial chamber opens to outside. It remains unknown whether there is a dermal plate covering the oralobranchial fenestra. The anterior oral fenestra (or.f, Fig  3B) looks like an inverted funnel with a maximum width of 20.5 mm, and a maximum length  Fig 3B) is suborbicular in shape with the maximum width of 41.0 mm, and the maximum length of 30.1 mm. There are 5-7 successive notches probably for the external branchial opening (br.o, Fig 3B) along the margin of the branchial fenestra, which exhibits a non-polybranchic condition comparable to that of basal galeaspids Hanyangaspis, Xiushuiaspis, and the Eugaleaspiformes. In the last two branchial notches, two small canals are preserved probably for the afferent branchial artery or efferent branchial vein (abr.a/ebr.v, Fig 3C) issuing from the marginal vein or artery which is only identified in galeaspids and osteostracans [45]. Posterior to the oralo-branchial fenestra, the ventral rim protrudes inside as a medial process to embrace the fenestra caudally.
The densely-distributed canal network probably for the subcutaneous vascular plexi (va.p, Fig 3C) is discernible between the exo-and endoskeletons on the left part of ventral rim of the holotype. The ornamentation of the head-shield is composed of coarse-granular tubercles as seen in the holotype (Fig 4A and 4B).
Nanningaspis zengi. It is a large sized polybranchiaspiform, which is about three times Platylomaspis serratus in size. The pear-shaped head-shield tapers rostrally into a long rostral process. The maximum preserved length of the head-shield (including the rostral process) is 183.3 mm, the maximum preserved width is 137.0 mm. The maximum width of the headshield is estimated to be 167.0 mm. The rostral process is incomplete, but its preserved distal extremity suggests that it tapers anteriorly. The length of the rostral process is 82.8 mm ( Fig  5A-5C).
The dorsal side of the head-shield shows a dome-shaped roof with a very broad flatten margin. The median dorsal opening is partly preserved in the anterior part of head-shield, showing vaguely an oval shape as in other polybranchiaspiforms. A small opening in anterolateral part of the dome probably represents the orbital opening. It is elliptic in outline with long axis aligned with the rostro-caudal axis, and the long and short axes are 10.0 mm and 5.3 mm respectively. Compared with other polybranchiaspiforms, the orbital opening of Nanningaspis has a more lateral position and faces anterolaterally rather than dorsally (Fig 5A-5C).
Like Platylomaspis, Nanningaspis has a very broad ventral rim as inferred from the broad flattened margin of the dorsal side of the head-shield. The ventral rim gradually widened from caudal to rostral with the preserved minimum width of 25.8 mm, and the maximum width of 40.6 mm. The lateral margin of the head-shield is smooth without serrations (Fig 5A-5C).
The ornamentation of the head-shield is composed of coarse-granular tubercles (Fig 5A-5C).

Comparisons
Platylomaspis is more comparable to Gumuaspis in the pear-shaped head-shield, short rostral process, and leaf-shaped inner corners. Platylomaspis differs from Gumuaspis mainly by its much broader ventral rim, much larger inner corner, and less branchial fossae. In addition, the lateral margin of the dorsal head-shield is smooth in Gumuaspis, but serrated in Platylomaspis. The ornamentation of head-shield is composed of stellate tubercles in Gumuaspis in contrast to coarse-granular tubercles in Platylomaspis (Figs 2A, 3 and 4).
Nanningaspis resembles Gumuaspis and Platylomaspis in bearing a pear-shaped head-shield with a rod-like rostral process and without laterally projecting corner (Figs 2A and 3-5). In comparison to Gumuaspis, Nanningaspis is more similar to Platylomaspis in having a broad ventral rim and coarse-granular tubercles. Nanningaspis differs from Platylomaspis in having over two times larger sized head-shield (Figs 3-5). Galeaspids display little size variation, e.g. apart from a slight variation in size, no clear growth series has ever been observed from the rich collection of Polybranchiaspis liaojaoshanensis in Yunnan and Shuyu zhejiangensis in Zhejiang [3,46]. In addition, the lateral margin of the head-shield is serrated in Platylomaspis, but smooth in Nanningaspis. Although the median dorsal opening, orbital opening, and sensory canal system remain unclear in both taxa, the available information indicates that the orbital opening of Nanningaspis is more laterally set than that of Platylomaspis, and probably faces anterolaterally (Figs 3-5).

Phylogenetic results
To explore the phylogenetic positions of Platylomaspis and Nanningaspis, we conducted phylogenetic analysis using the dataset of Zhu and Gai [4] with the addition of Character 54, broad ventral rim: absent (0), present (1)(S1 File). The character data entry and formatting were performed in Mesquite (version 3.31). All characters were treated as unordered and weighted equally, as in the earlier versions of this dataset. A basal osteostracan Ateleaspis is selected as outgroup for the phylogenetic analysis. The analysis was conducted using PAUP 4.0a, parsimony analysis package, using the heuristic search option (1000 replicates, random addition sequence) [42]. Total number of rearrangements tried = 1.5515e+10. This analysis yielded three equally most-parsimonious trees with a tree length = 134, consistency index (CI) = 0.5038, retention index (RI) = 0.7959 (S2 File).
These three equally most-parsimonious trees differ only in the positions of 3 Silurian genera Hanyangaspis, Changxingaspis and Dayongaspis and yield three major clades as defined in earlier works, namely the Eugaleaspiformes, Polybranchiaspiformes and Huananaspiformes. Platylomaspis and Nanningaspis fall into the Polybranchiaspiformes. Within Polybranchiaspiformes, Platylomaspis is resolved as the sister group of Nanningaspis by the synapomorphy of the broad ventral rim. They are consistently clustered with Gumuaspis to form a monophyletic group, Gumuaspidae fam. nov., and positioned at the base of Polybranchiaspiformes (Fig 6).

Temporal and palaeobiogeographic distribution of Polybranchiaspiformes
Our phylogenetic analysis indicates that the Gumuaspidae occupies the most basal position of Polybranchiaspiformes. The Polybranchiaspiformes is a highly diverse group of galeaspids, which was assumed to occur from the Lochkovian to the Eifelian [4,47]. The earliest fossil record of Polybrachiaspifomes was from the Xishancun Formation (Lochkovian) including Pentathraspis, Polybranchiaspis, Laxaspis, Damaspis, Pseudolaxaspis, Diandongaspis, and Altigibbaspis [43,[48][49][50][51]. The high diversity of Polybrachiaspifomes in the Xishancun Formation indicated that the Polybrachiaspifomes should have differentiated earlier. The finding of Platylomaspis from the Telychian of Llandovery, Silurian (~438 million years ago) extends the earliest occurrence of Polybranchiaspiformes backward about 19 million years (Fig 7). By the Pragian, the diversity of Polybranchiaspiformes suddenly declined. Except for the Gumuaspidae, there are only two unusually large-sized polybranchiaspiforms: Bannhuanaspis vukhuci from the uppermost part of the late Lochkovian to the early Pragian (Si Ka Formation of Bac Bo, Vietnam) and Dongfangaspis major from the Pragian (Pingyipu Formation of Sichuan) survived [43,52]. The Gumuaspidae, as the longest lasting galeaspid family from the Telychian to the Pragian (~407 million years ago), witnessed the three major radiations of galeaspids: i.e. basal galeaspids plus Eugaleaspiformes (box A, Fig 7) in the Telychian, Polybranchiaspiformes in the Lochkovian (Node, B, Fig 7) and Huananaspiformes in the Pragian (Node, C, Fig 7).
The findings of Platylomaspis and Nanningaspis also have important palaeobiogeographic significance. For the Galeaspida, a correlation between phylogeny and palaeobiogeography is observed, e.g. a close association between Tarim and the southeastern China region is New Silurian and Devonian jawless fishes reconstructed by the phylogeny of 'basal' galeaspids [53]. Over the last decades, the polybranchiaspiforms have been assumed to be endemic to southern China and northern Vietnam, never found in Tarim [4,47]. The earliest occurrence of Platylomaspis in the Silurian Tarim Basin expands the distribution of polybranchiaspiforms from South China to Tarim (Fig 1A). As galeaspids are heavily armoured bottom-dwellers with limited locomotory and dispersal capabilities, the continents and open oceans became the major obstacles for their migration and dispersal [53,54]. Geological and paleontological evidence indicates that the South China, the North China and the Tarim blocks were very close during the Llandovery of Silurian. They were neighboured each other, and shared a highly endemic vertebrate fauna, namely "the Pan-Cathaysian Galeaspid Fauna" [54]. The finding of Platylomaspis in Tarim and Nanningaspis in Guangxi corroborates that a united Tarim-South China Block existed in the Llandovery and Wenlock of Silurian [18,20,54], and provides a potential route for early polybranchiaspiforms migrating from the Tarim Block to the South China Block. The united block was probably divided into two blocks in the Ludlow or later, when the Tarim Block began to drift away to the northwest [18].

Ecomorphological implications
Platylomaspis and Nanningaspis are characterized by a pear-shaped head-shield with a rostral process and a unique broad ham-brim-like ventral rim. The rostral process, which was regarded as a diagnostic character of Huananaspiformes [55] are also present in other two major groups of galeaspids, e.g. Gumuaspidae in Polybranchiaspiformes, Eugaleaspidae in Eugaleaspiformes [44,56]. The rostrum of galeaspids might have the same functions as the rostrum of other agnathans that lived under similar environmental conditions, e.g. boreaspidid osteostracans, pituriaspids, and amphiaspidid and pteraspidid heterostracans [3,[57][58][59]. It may have a sensory function in search of food or a hydrodynamic function [60,61]. However, the hypothesis of the protective function of the rostrum by scaring predators in osteotracans proposed by Janvier [62] are probably not applicable to Gumuaspidae as it lacks other processes, e.g., the laterally directed cornual processes which can largely intensify the effect of protective function. The recurrent evolution of similar rostral processes in various agnathan groups probably indicates similar ecological pressures upon the clades and diversifications into comparable niches [63].
The wide-brimmed head-shield is a diagnostic character uniquely shared by Platylomaspis and Nanningaspis (Fig 8A). The morphological adaptations of galeaspids, e.g. a strongly flattened head-shield, dorsally-positioned eyes and 'nostril', suggested that they are coastal bottom-dwellers living in the habitats close to palaeocontinental margin, including delta and estuary [3,54]. Like modern Cottidae and Gobiesocidae, the strongly armored and flattened head-shield of galeaspids provides greater density and helps the fish maintain contact with the substratum in moderate currents without a significant energetic cost [9,64]. Therefore, the extreme flattening of the head-shields strengthened by the wide ventral rim in Platylomaspis and Nanningaspis at least can help them better adapt to the benthic habitat or have a selective advantage when living in an environment dominated by strong currents. Here, we further show that the wide-brimmed head-shield with a rod-like rostral process in Platylomaspis and Nanningaspis is probably analogous to the pectoral disc and long duck-billed snout in modern rays (Fig 9), e.g. Dasyatis and Rajella [65]. The head shape of the latter facilitates them to burrow underneath the sands in order to avoid predators and wait for feeding opportunities.
Galeaspids have a remarkable character combination to make breathing and feeding more effective. Like modern rays, galeaspids have their mouth and gill openings on the ventral side of head-shield (Fig 9B, m, br.o), but have a diagnostic large median dorsal opening for the nostril located on the anterodorsal side of head-shield (md.o, Fig 9A). The synchrotron radiation X-ray tomographic study revealed that the large median dorsal opening penetrated the roof of oral cavity to communicate with the oral cavity and served for the main water intake device [1]. The diagnostic median dorsal opening of galeaspids is probably analogous to the spiracle in modern rays (Fig 9C, spi) through which the oxygenated water is inhaled. After oxygen is exchanged in gills, the deoxygenated water is exhaled though the ventral branchial opening. This is a useful adaptation for a benthic animal because it can respire oxygen without any need to move and have its mouth free for feeding. Janvier [3] and Pernègre [66] considered that the water intake device in some jawless fishes, e.g. the nasopharyngeal duct in hagfishes (na.p, Fig  10B), the dorsally positioned mouth (m, Fig 10C) and the adorbital openings (ad.o, Fig 10D) in pteraspid and amphiaspid heterostracans, as well as the median dorsal opening in galeaspids (md.o, Fig 10E) probably linked with the partial burrowing habits.
However, this hypothesis of the partial burrowing habit is not suitable for most galeaspids because their median dorsal openings are so subterminal, or even terminal, that the silt and mud would pour into oralobranchial chamber when they were buried to inhale water (Fig 8B). Being choked while hiding in sands means that the galeaspids cannot hide themselves in sands and are subject to the danger of predators. By contrast, the wide-brimmed head-shield and rostral process in Platylomaspis and Nanningaspis had set the median dorsal opening and orbital openings highly in the middle of the head-shield, which not only makes the head-shield easy to be buried in silt or mud, but also can keep eyes and the large median dorsal opening outside for vision and respiration (Fig 10E and 10F). In this case, the rostral process could possibly function as a detector of potential food in silt or mud like the long duck-billed snout of modern rays. In addition, the coarse ornamentation and serrated lateral margin in Platylomaspis would play a role in resisting the tendency to slide backwards while moving forward through the sediment as proposed in some ctenaspid heterostracans (sl.b, Fig 10D and 10E) [67,68]. Hence, we propose that the unique wide-brimmed head-shield together with the rostral process in Platylomaspis and Nanningaspis is most likely to be a special adaptation for a semi-infaunal benthos, representing a partial burrowing habit in galaeaspids. With the semiinfaunal habit, Platylomaspis and Nanningaspis were better hidden from predators than epifaunal galeaspids and could stay in moderate currents with less energetic cost.
Recent studies indicated that the ventral rim of galeaspids had undergone an opposite modification in the Gantarostrataspidae [9], a clade of Huananaspiformes. Compared to the very broad ventral rim in Platylomaspis and Nanningaspis, the ventral rim of the Gantarostrataspidae is largely reduced, and even completely lost to form a streamlined torpedo-like head-shield ( Fig 8C) to minimize the water drag by reducing the magnitude of the pressure gradient over the body [9]. This indicates that some galeaspids such as Rhegmaspis probably became more effective swimmer for a suprabenthic or nektonic habit (Fig 11, up). As such, the long rostral process was probably used as a scraper to plow the bottom in a relatively high speed to stir digestible materials for a more efficient filtering than the epibenthic galeaspids [9]. The discoveries of Nanningaspis from the Nakaolin Formation, Dongfangaspis from the Pingyipu Formation, and Rhegmaspis from the Posongchong Formation indicate that the demersal galeaspids had further developed three different kinds of habit types: semi-infaunal benthic (half buried) (Fig 10 bottom), epibenthic (Fig 11, middle), and suprabenthic (nektonic) (Fig 11, up) habits to occupy different vertical ecological niches by the Pragian of the Early Devonian. Coupled with such a habit differentiation, galeaspids reached the peak of their diversity during the Pragian [69]. A. a modern southern stingray Dasyatis using its spiracle for the intake of respiratory water when buried in sand (photo courtesy by Eugene Kitsios/Bimini Biological Field Station Foundation, http://www.kitsios-photography.com); B. the modern Pacific hagfish Eptatretus using its nasopharyngeal duct for the intake of respiratory water when buried in mud; C. the pteraspid heterostracan Doryaspis probably using its dorsal set mouth for the intake of respiratory water when buried in sand; D. the amphiaspid heterostracan Kureykaspis using its adorbital opening for the intake of respiratory water when buried in sand; E, F. the gumuaspid galeaspid Platylomaspis using its median dorsal opening (nostril) for the intake of respiratory water when buried in sand (B-F illustrated Dinghua Yang). Abbreviations: ad.o, adorbital opening; br.o, common external branchial opening; m, mouth; md.o, median dorsal opening; na.p, nasopharyngeal duct; orb, orbital opening; ro, rostral process; sl.b, serrated lateral brim; spi, spiracle.