Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave

Flavonoids are secondary metabolites of plants that often have medical applications. The influences of different sample drying pretreatments on flavonoids and antioxidant activity of ferns have not studies. Dryopteris erythrosora leaves used to analyze flavonoid alterations resulting from drying pretreatments. The total flavonoid content of D. erythrosora leaves exposed to different pretreatments was significantly different. The total flavonoid content of samples initially air-dried in shade and then oven-dried at 75°C were the highest (7.6%), while samples initially dried at 75°C had the lowest content (2.17%). Antioxidant activities of D. erythrosora leaves with different pretreatments varied. Group B first air-dried in the shade and then oven-dried at 75°C and group C first air-dried in the sun and then oven-dried at 75°C, both showed relatively stronger antioxidant activity. The best pretreatment for preserving the flavonoids was to first dry the plant material in the shade and then complete the drying process in an oven at 75°C. It was tentatively identified 22 flavonoids among the four different pretreatments by HPLC-ESI-TOF-MS.


Introduction
Flavonoids are plant secondary metabolites with medical applications [1]. Some factors influenced flavonoid levels, such as harvest time [2][3][4], shade netting, planting time [5], development [6], and using the light transmittance paper bags [7]. Sample processing can influence the quantity and quality of bioactive compounds [8][9][10]. For example, the flavonoid content of fresh mulberry leaves was highest and the content in leaves that were oven-dried at 100-105˚C was lowest [11]. Flavonoid content, DPPH scavenging activity, and reducing power of Salvia officinalis L. leaves dried in the shade were higher than levels in leaves oven-dried at 65˚C [12]. Paramignya trimera dried in an oven at 25˚C had a higher flavonoid content than samples dried in an oven at 100˚C [8]. Flavonoid yields differed in Belamcanda chinensis dried at a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 temperatures ranging from 40˚C to 120˚C [13]. These studies demonstrated that drying conditions could alter the flavonoid content and biological activity of plant flavonoids.
However, the manner in which different drying pretreatments affect flavonoid levels remains unclear. Ferns generally have high flavonoid contents but there is little information on the effects of drying pretreatments on flavonoid levels. Therefore, Dryopteris erythrosora (Eaton) O. Ktze. (Dryopteridaceae) used for the analysis of flavonoids. The aims of this study were to: (I) assess the effects of different drying pretreatments on the flavonoids and the antioxidant activity of D. erythrosora leaves, and (II) determine the best drying pretreatment for conserving fern flavonoids and antioxidant activity.

Preparation of plant extracts
Fresh leaves of D. erythrosora were randomly separated into four groups and then exposed to four treatments. Group A leaves cleaned and frozen in liquid nitrogen were pulverized. Group B leaves were initially dried in the shade about one day then oven-dried at 75˚C for 48 h, before grinding them to a powder. Group C leaves were initially dried in the sun (on top of absorbent old newspapers), then oven-dried at 75˚C for 48 h, then ground to a powder. Group D leaves were cleaned then initially oven-dried at 75˚C for 48 h prior to grinding. Ground samples were passed through an 80-mesh screen.
Powders (1.0 g) from groups B, C, and D were separately added to 60% ethanol (25 mL) and disposed with ultrasound machine-assist (20 min) and then water-bath at 50˚C for 2 h. The extraction process repeated twice, and the mixture was filtered using a vacuum suction filter pump with the solution volume maintained at 50 mL. To obtain the same dry weight, 3.3 g samples from group A ground in a mortar and then added to 60% ethanol (25 mL) for extraction. Extraction was prepared as the above.
One portion of the extract used for determining the total flavonoid content and antioxidant activity. The other portion was extracted by petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc), and n-butanol (nBuOH) for HPLC-ESI-TOF-MS analysis of flavonoids.
Antioxidant activity DPPH assay. The DPPH free radical scavenging activity assay was based on information in a previous study [14]. Briefly, 1 mL DPPH (0.1 mM in ethanol) and extracts with a gradient of concentrations were mixed. After incubation for 30 min, the absorbance value was measured at 517 nm. A 60% methanol sample was used as the control group. The DPPH free radical scavenging activity was calculated using the following formula: DPPH free radical scavenging activity (%) = (1-A sample 517 /A control 517 ) � 100. The experiments were performed in triplicate (RSD < 5.0%).
ABTS assay. The ABTS assay of the extracts was previously described [14]. Briefly, 150 μL extracts with gradient concentration and 3 mL of appropriately diluted ABTS solutions were mixed. After incubating for 6 min, the absorbance value at 734 nm was determined. The ABTS free radical scavenging activity was calculated by: ABTS free radical scavenging activity (%) = (1-A sample 734 /A control 734 ) � 100. The experiments were performed in triplicate (RSD < 5.0%).

Flavonoid analysis of D. erythrosora leaves with different drying pretreatments using HPLC-ESI-TOF-MS
Chromatographic separation was performed on an Agilent 1100 HPLC system (USA Agilent Technologies), equipped with a binary pump, a microdegasser, Hi-performance well-plate auto sampler, thermostat column compartment, and diode-array detector (DAD). UV spectra were recorded between 190 and 400 nm, and the UV detector was set at 254 nm. Separation was performed on a SHISEIDO MG-C18 (1003.3 mm; i.d. 3.0 mm) column using a gradient elution [methanol (A)/ water (0.1%HCOOH)(B)].
Extractions of petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc), and n-butanol (nBuOH) were each diluted 10 times. The gradient program was 0-15 min, 15-45% A; 15-25 min, 45-55% A; 25-35 min, 55-90% A; the flow rate maintained at 0.4 mL/min, and the sample injection volume was 10 μL. The column temperature was set at 25˚C. All of the MS experiments conducted on an Agilent 6220 Time-of-Flight mass spectrometer (TOF) equipped with an electrospray ionization (ESI) interface (Agilent Technologies, USA). Both the auxiliary and nebulizer gases were nitrogen with flow rates of 10 L/min. The MS analysis was performed in both positive and negative scan modes under the following operation parameters: dry gas temperature = 350˚C, voltage = 160V and the nebulizer pressure = 45 psi. Full scan data acquisition and dependent scan event data acquisition were performed from m/ z 100-1200.

Total flavonoid content of D. erythrosora leaves with different drying pretreatments
Datas from the standard curve of Rutin were list in S1 Table. The total flavonoid contents of D. erythrosora leaves with different drying pretreatments were 7.38% (Group A), 7.6% (Group B), 6.41% (Group C), and 2.17% (Group D), respectively (Fig 1). The total flavonoid content of extracts from group B, which were first dried about one day then completely oven-dried at 75˚C was the highest. The flavonoid content from group D, dried at 75˚C in oven directly after cleaning, was the lowest.
The process of drying in the shade produced a slow rate of water loss.which might result in the increasing of total flavonoid content. Sun-drying produced a faster rate of water loss, ending flavonoid metabolism. Full sunlight exposure can influence flavonoid metabolites in leaves [15][16], which results in a decreased flavonoid content. Samples from group A, which were ground with liquid nitrogen, showed the total flavonoid content similar to the live samples. We speculate that water and sunlight are the main factors affecting total flavonoid content in leaf samples. In addition, flavonoids are heat sensitive [17]. Heating at 75˚C directly can destroy enzyme activity and block the synthesis pathway of flavonoids. This may be the reason why the total flavonoid content of Group D was the lowest. Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave

Antioxidant activity
The DPPH free radical scavenging activities of D. erythrosora leaf extracts with different pretreatments were shown in Fig 2. When the doses were from 0-80 μL, the DPPH free radical scavenging potential increased. Group C was higher than group B. Fewer than 10 μL of the extracts could scavenge about 50% of the free radicals. However, group D was significantly lower than group C. The IC 50 of groups A, B, C, and D were 0.6 μg/mL, 0.38 μg/mL, 0.25 μg/ mL, and 0.35 μg/mL, respectively. The DPPH free radical scavenging activity of the extracts was arranged as group C>group B>group A>group D. The ABTS free radical scavenging activities are shown in Fig 3. When the volume was between 0-150 μL, the ABTS free radical scavenging potential increased. Group B was the highest and group D the lowest. A 120 μL amount of the extract scavenged about 50% of the free radicals. The IC 50 of groups A, B, and C were 4.62 μg/mL, 3.72 μg/mL, and 3.52 μg/mL, respectively. The ABTS free radical scavenging potential of the groups were: B> C> A> D The superoxide anion scavenging activity showed in Fig 4. In the range from 10-90 μL, the superoxide anion scavenging potential increased. The samples that were first dried in the sun and then oven-dried at 75˚C had relatively stronger superoxide scavenging activities. The IC 50 values of groups A, B, and C were 1.81 μg/mL, 2.28 μg/mL, and 1.25 μg/mL, respectively.
The FRAP assay and reducing power assay results are shown in Figs 5 and 6. Extracts from D. erythrosora leaves with different drying pretreatments possessed both antioxidant and reductive activity of Fe 3+ . With increased volume, the activity increased. The activity level between groups was: B> C> A> D.
These results illustrated that different drying pretreatments altered the antioxidant activities of D. erythrosora leaves. Except for the superoxide anion (O 2-) scavenging assay, groups B and C both had stronger antioxidant activity than group D, which might be related to the flavonoid contents.

Flavonoids analysis of D. erythrosora leaves with different drying pretreatments using HPLC-ESI-TOF-MS
HPLC-ESI-TOF-MS was used for the qualitative analysis of flavonoids of D. erythrosora leaves. Comparing the data with known chromatograms and mass spectral data, a total of 22 peaks were tentatively identified as flavonoids (Table 1). There were 8 flavonols, 4 flavones, 3 chalcones, 2 flavanols, 2 flavanones, 1 homoisoflavones, 1 isoflavone, and 1 isoflavanone in the mass spectrometry-total ions chromatogram (MS-TIC) of extracts from D. erythrosora leaves with different drying pretreatments in the negative ion mode. The main flavonoids of D. erythrosora leaves were flavone and flavonols, and this result is consistent with previous studies [18][19][20][21][22]. Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave Chalcones and isoflavones were absent in group A but present in groups B and C. This is the first report of these compounds in the Dryopteridaceae. This result showed chalcones and isoflavones could not be synthetized during natural growth of D. erythrosora leaves but could result from the stresses of sun-drying or shade-drying. Except for anthocyanin and its derivatives, all of the flavonoid types in the flavonoid synthesis pathway could be found [23]. We concluded that the flavonoid metabolic pathways of ferns are similar to spermatophytes and that the metabolic pathways are closely related to the stress response.
Loss of flavonoids from group D was the greatest. Figs 7 to 10 show that scutellarein 7-Oglucobioside, apigenin 7-O-rutinoside, apigenin-C-pentoside, and kaempferol 3-O-α-L-arabinopyranoside are common flavonoids. This suggests that the flavonoid biosynthesis of the Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave four components was unaffected by the drying pretreatments. However, the contents of the four flavonoids significantly changed (Fig 11). We used Group A samples (frozen-ground in liquid nitrogen) as the standard and found that the content of scutellarein 7-O-glucobioside increased. The largest amounts of these compounds were from group D. This result indicated that heating directly accelerated the synthesis of scutellarein 7-O-glucobioside.
The contents of apigenin 7-O-rutinoside and kaempferol 3-O-α-L-arabinopyranoside both decreased, and the lowest content was in group D. This demonstrated that the two compounds might decompose or transformed with direct heating. In addition, the amounts of apigenin-Cpentoside of groups B and C increased, but decreased in group D. This suggests that temperature might be the main factor influencing the synthesis of apigenin-C-pentoside. Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave Among groups A, B, and C, 11 common flavonoids were found. The contents of these flavonoids also changed (Fig 12). The key difference in the drying pretreatments between groups B and C was the dehydration rate, which resulted in a myricetin 3-O-glucoside, kaempferide 3-rhamnoside-7-(6"-succinylglucose), and isocarthamidin-7-O-glucuronide increase but a reduction in quercetin-3-O-β-D-xylopyranoside and apigenin-6-C-ara-8-C-glu. The content of koreanoside B changed little, indicating that the synthesis of koreanoside B was not influenced. The decreased rutin may have resulted from the decomposition or the transformation.  The number of flavonoids was greatest in group B. Compared to group A, group B had 9 additional flavonoids and the contents of 8 common compounds increased. The levels of kaempferide 3-rhamnoside-7-(6"-succinylglucose) were 2× greater than group A.
High light irradiance can influence the biosynthesis of dihydroxy B-ring-substituted flavonoids [24][25][26]. In this study, the effects of light irradiance were not obvious. Among the different drying pretreaments, the loss of flavonoids in group D was the greatest (67% lost).

Conclusion
This study examined the influences of different drying pretreatments of D. erythrosora leaves on total flavonoid contents, antioxidant activity, and flavonoid ingredients. The main conclusions were: a) The total flavonoids contents, antioxidant activities, and flavonoid ingredients in leaves with different drying pretreatments varied, and samples that were first dried in the shade then oven-dried at 75˚C had the highest flavonoid content and strongest antioxidant activities. b) The pretreatment of rapid oven drying in a 75˚C oven produced the greatest loss of flavonoids. The most successful pretreatment for conserving leaf flavonoids was the initial shade drying followed by oven drying at 75˚C. Freezing and grinding in liquid nitrogen followed by filtering was also a useful technique.
Supporting information S1