Susceptibility of influenza viruses to hypothiocyanite and hypoiodite produced by lactoperoxidase in a cell-free system

Lactoperoxidase (LPO) is an enzyme found in several exocrine secretions including the airway surface liquid producing antimicrobial substances from mainly halide and pseudohalide substrates. Although the innate immune function of LPO has been documented against several microbes, a detailed characterization of its mechanism of action against influenza viruses is still missing. Our aim was to study the antiviral effect and substrate specificity of LPO to inactivate influenza viruses using a cell-free experimental system. Inactivation of different influenza virus strains was measured in vitro system containing LPO, its substrates, thiocyanate (SCN-) or iodide (I-), and the hydrogen peroxide (H2O2)-producing system, glucose and glucose oxidase (GO). Physiologically relevant concentrations of the components of the LPO/H2O2/(SCN-/I-) antimicrobial system were exposed to twelve different strains of influenza A and B viruses in vitro and viral inactivation was assessed by determining plaque-forming units of non-inactivated viruses using Madin-Darby canine kidney cells (MDCK) cells. Our data show that LPO is capable of inactivating all influenza virus strains tested: H1N1, H1N2 and H3N2 influenza A viruses (IAV) and influenza B viruses (IBV) of both, Yamagata and Victoria lineages. The extent of viral inactivation, however, varied among the strains and was in part dependent on the LPO substrate. Inactivation of H1N1 and H1N2 viruses by LPO showed no substrate preference, whereas H3N2 influenza strains were inactivated significantly more efficiently when iodide, not thiocyanate, was the LPO substrate. Although LPO-mediated inactivation of the influenza B strains tested was strain-dependent, it showed slight preference towards thiocyanate as the substrate. The results presented here show that the LPO/H2O2/(SCN-/I-) cell-free, in vitro experimental system is a functional tool to study the specificity, efficiency and the molecular mechanism of action of influenza inactivation by LPO. These studies tested the hypothesis that influenza strains are all susceptible to the LPO-based antiviral system but exhibit differences in their substrate specificities. We propose that a LPO-based antiviral system is an important contributor to anti-influenza virus defense of the airways.


Introduction
Influenza virus epidemics and periodic pandemics affect millions of people worldwide causing serious substantial morbidity and mortality representing a major economic burden [1,2]. Current prophylactic options are problematic due to the yearly need for strain-specific vaccination, the development of drug resistance, and changes in the virus related to antigenic drift and viral reassortment [3][4][5]. Identifying novel antiviral approaches that are broadly effective against several influenza strains is urgently needed. In this regard, the respiratory innate immune system could possess such mechanisms.
Bronchial epithelial cells (BEC) are a primary target for influenza virus replication [6]. Early response by BECs to influenza virus is crucial in determining progression of viral infection, adaptive immunity, and lung pathogenesis [7]. BECs orchestrate an oxidative extracellular antimicrobial system present in the airway surface liquid consisting of LPO, its main natural substrate, the thiocyanate anion (SCN -) and hydrogen peroxide (H 2 O 2 ) (Fig 1) [8][9][10][11]. LPO is an abundant peroxidase in the airways that uses H 2 O 2 to oxidize its most abundant and preferred substrate, SCN -, into hypothiocyanite (OSCN -) [12]. Hypothiocyanite is a shortlived, antimicrobial agent that is not toxic to the host and has been shown to kill several microbes, mainly bacteria. Its antiviral action remains, however, less characterized and understood.
Hypothiocyanite was to shown to inactivate the A/H1N1 2009 pandemic influenza virus in a cell-free system [13]. We also reported that OSCNgenerated on the apical surface of BECs is capable of inactivating the A/swine/Illinois/02860/09 (swH1N2) influenza A strain [9]. These  observations suggest that the H 2 O 2 /LPO/SCNsystem is an important contributor to antiinfluenza viral defense of the respiratory immune system, and imply that this innate response could be utilized to enhance early clearance of influenza virus perhaps in a strain-independent manner. To better understand the anti-influenza virus action of this oxidative system, we aimed at characterizing its virus-inactivating effect by testing several influenza virus strains. In addition to its natural substrate, SCN -, we also tested iodide (I -), as LPO substrate to study the substrate specificity of the antiviral action of LPO. LPO readily oxidizes several halides and pseudohalides using H 2 O 2 [14]. Iodide is considered to be a better LPO substrate than SCNto inhibit growth of certain fungi and bacteria [15,16]. In this study, we tested an in vitro, cellfree experimental system to characterize the anti-influenza virus action and substrate specificity of LPO. We found that LPO inactivates a wide-range of influenza strains, but surprisingly the extent of LPO-mediated viral inactivation and LPO substrate preference differed among tested isolates.

Influenza A and B strains
Influenza A and B strains used in this work were purchased from the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH (BEI Resources) and are listed in Table 1. Influenza viruses were propagated in Madin-Darby Canine Kidney (MDCK) cells (ATCC1 CCL-34™) as described [9]. Briefly, MDCK cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing high glucose (HyClone) supplemented with 5% heat-inactivated fetal bovine serum (FBS; HyClone) and maintained at 37˚C with 5% CO 2 . Viral strains were cultured in MDCK cells using infection medium (DMEM containing high glucose supplemented with 1 mM L-glutamine with 1-μg/ml tosylsulfonyl phenylalanyl chloromethyl ketone [TPCK]-treated trypsin). Viruses were collected 24-48 hours post-infection.

Cell-free viral inactivation assay
Components of the LPO-based antiviral system were used at the following concentrations: 6.5 μg/ml LPO, 400 μM SCN -/I -, glucose (0.005 M) and glucose oxidase 0.01 U/mL. The reaction volume was set to 40 μL with the appropriate concentration of each component was also used when indicated to inhibit the system. The components were assembled in a sterile Eppendorf tube with the virus being added last. The tubes were then placed on a 37˚C heating block for one hour. After the incubation, supernatants were stored at -80˚C. Plaque assays were performed on MDCK cells to determine viral concentrations as previously described [9,17]. This assay is the "cell-free" version of the viral inactivation assay established previously on primary airway epithelial cells with the main difference that the source of H 2 O 2 here is the glucose/GO enzymatic reaction compared to Dual oxidase 1 in epithelial cells [9].

Statistics
Data for the viral inactivation assay were log 10 -transformed and significance was calculated using a one-way ANOVA followed by Tukey or Dunn's multiple comparison post-hoc test when more than two samples were compared. When virus inactivation or the substrate preference ratios were compared between two LPO substrates or influenza subtypes or species, Mann-Whitney test was used. Statistical analysis was performed using Prism 6 for Windows version 6.07 software. Ã , p<0.05; ÃÃ , p<0.01; ÃÃÃ , p<0.001.

Optimization of the cell-free, in vitro H 2 O 2 /LPO/(SCN -/I -) experimental system
LPO is produced by submucosal glands and epithelial cells in the respiratory tract and it accumulates in the airway surface liquid [18,19]. LPO concentration in airway secretions has been estimated to be in the range of 3-12 μg/ml [18]. We used a physiologically relevant LPO concentration of 6.5 μg/ml in our cell-free assay [20]. Thiocyanate is present in airway secretions in submillimolar concentrations [12,18]. Accordingly, we used 400 μM SCNin the in vitro assay, and used 400 μM iodide as alternative LPO substrate. The same LPO and SCNconcentrations were used as previously published in our prior studies using primary bronchial epithelial cells to make results comparable between the cell-free system and epithelial cultures [9]. While H 2 O 2 has been detected in airway secretions of humans and other mammalian species, estimating its basal levels is difficult due to its volatile nature. The main in vivo sources of H 2 O 2 in the airways are NADPH oxidases called Dual Oxidase 1 and 2 (Duox 1/2) [8]. Duox enzymes are highly expressed in the apical plasma membrane of BECs and generate H 2 O 2 directly into the airway surface liquid [8]. In our in vitro system containing BECs, Duox enzymes generate H 2 O 2 driving the antimicrobial action of LPO [9,20,21]. In the cell-free system described here, H 2 O 2 is provided by the enzymatic reaction of glucose (5 mM) and glucose oxidase (GO) that produces D-gluconic acid as the end product (Fig 1) [9]. Next we wanted to confirm this in the cell-free system. As shown in Fig 2A, the H 2 O 2 /LPO/SCNcell-free system had also a strong and substantial inactivating effect on the A/Swine/Illinois/02860/2009 (H1N2) strain. Importantly, viral inactivation in the cell-free system was entirely blocked by the addition of catalase, a H 2 O 2 -scavanging enzyme (Fig 2A). No viral inactivation was observed when only GO was added but LPO and SCNwere omitted from the system (Fig 2A). Similarly, having LPO and SCNwithout the H 2 O 2 -generating GO had no substantial viral-inactivating effect (Fig 2A). Comparable data were observed on BECs [9] indicating that H 2 O 2 alone is inefficient and OSCNis responsible for inactivating the H1N2 influenza strain in both systems. Next, we tested how H1N2 inactivation by the cell-free system changes if I -(an alternative LPO substrate) is used. Ihas been shown to enhance the antiviral activity of LPO against adenovirus and respiratory syncytial viruses [15,22]. Iwas used at the same concentration as SCN -(400 μM). Replacing SCNwith Iin the cell-free system led to even more effective H1N2 inactivation (Fig 2A). Similar to results obtained with SCN -, catalase blocked viral inactivation when Iwas used ( Fig 2B). Omitting any of the components of the Ibased cell-free system resulted in complete loss of H1N2 inactivation confirming that hypoiodite (OI -) is responsible for the virucidal effect ( Fig 2B). To better characterize the substrate preference of LPO in inactivating H1N2, we tested viral inactivation at doses of SCNand Ilower than 400 μM. As the dose-dependence curves shown in Fig 2C indicate, H1N2 was more susceptible to Imediated inactivation by the cell-free system. Interestingly, sensitivity to OSCNdrops drastically at SCNdoses just below 100 μM ( Fig 2C). Results presented here confirm that the cell-free system serves as a good model to study influenza inactivation by the H 2 O 2 /LPO/SCNsystem, and the swine H1N2 strain tested is susceptible to both, OSCNand OImediated inactivation by LPO, with a slightly higher sensitivity to OI -.

H1N1 influenza A strains are inactivated by LPO in a substrateindependent manner
Influenza A viruses are classified based on two of their surface proteins, hemagglutinin (HA) and neuraminidase (NA), that also determine their antigenic specificities. Since the H1N1 and H3N2 serotypes have been commonly present in human epidemics and pandemics over the last century [23], it was important to compare several H1N1 and H3N2 strains in the cell-free system for LPO-mediated inactivation (  [23][24][25]. We tested A/Brisbane/59/2007 and observed a 1.31+/-0.42 (mean+/-S.E.M., n = 3) log (SCN -) and a 1.06+/-0.28 (mean+/-S.E.M., n = 3) log (I -) inactivation by the cell-free system, respectively (Fig 3A). The extent of OSCNmediated viral inactivation was significant (p = 0.011) while that of OIwas not (p = 0.090) (Fig 3A). Catalase had an inhibitory but non-significant (p = 0.092) and incomplete effect on viral inactivation for both substrates (Fig 3A). Both LPO substrates mediated A/California/04/2009 viral inactivation beyond detection limit of the assay in the cell-free system which was highly significant (p<0.0001, for each substrate) (Fig 3A). Removing H 2 O 2 by catalase treatment significantly reversed (p<0.0001) the virus-inactivating effect of LPO by both substrates (Fig 3A). The A/Mississippi/3/2001 strain was also efficiently inactivated by LPO in a substrate-independent manner (log-based extent of inactivation: 6.16+/-0.27 for OSCNwhile 6.88+/-0.55 for OI -, n = 4) that was entirely blocked by catalase (Fig 3A). Although the A/Turkey/Kansas/ 4880/1980 H1N1 strain was also efficiently inactivated by LPO, its extent did not reach levels of significance (Fig 3A). While the four H1N1 influenza strains tested did not show a strong substrate preference in terms of inactivation by the cell-free H 2 O 2 /LPO/(SCN -/I -) system, they differed in their susceptibilities ( Fig 3A). This is also shown in Fig 3B where the extent of virus inactivation is calculated as the catalase-dependent difference in viable viral titers (viral doses detected in presence of the full cell-free system are subtracted from viral doses recovered in presence of the full cell-free system plus catalase). Virus inactivation is calculated throughout the entire manuscript the same way.
To assess potential association of hemagglutinin or neuraminidase types of IAVs with their susceptibility to the LPO-based system, we compared viral inactivation and substrate preference of all nine IAV strains tested. When IAVs tested so far were grouped according to their HA types (H1 and H3) or NA types (N1, N2), no significant differences were seen between them regarding OSCNor OImediated viral inactivation (Fig 5A and 5B, left and middle panels). To better characterize the substrate preference of LPO-mediated viral inactivation, we calculated a "SCN -/ Isubstrate preference ratio" or "OSCN -/OIsusceptibility ratio" for each strain by dividing the extent of SCNfueled virus inactivation by the extent of the Imediated one. When these SCN -/Isubstrate preference ratios of all nine IAV strains were grouped according to their HA type (irrespective of NA), they were significantly (p = 0.0159) higher with H1 compared to H3 (Fig 5A, right panel). When the substrate preference ratios were grouped according to the NA type (irrespective of HA), we found no significant (p = 0.111) difference ( Fig 5B, right panel). In summary, H3N2 IAV strains are also susceptible to the antimicrobial actions of LPO and show a surprising preference for Ias the preferred substrate for inactivation that is associated with H1 and H3 hemagglutinin subtypes.

The influenza B virus strain, B/Yamagata/16/1988, is more susceptible to hypothiocyanite than hypoiodite
IBV strains primarily infect humans, do not have established animal reservoirs and pose little risk of pandemic infection [30,31]. IBV may cause seasonal influenza infections and co-circulate with IAV strains [30]. Importantly, IBV can dominate influenza seasons and is typically more resistant to antivirals than IAVs [30,32]. We investigated whether LPO present in our cell-free system can inactivate the influenza B strain, B/Yamagata/16/1988, a prototype strain of the Yamagata lineage of IBV strains [33]. As shown in Fig 6A, OSCNled to complete, highly significant (p<0.001) inactivation of the viral dose used. Using Ias the LPO substrate, viral inactivation of IBV was significant (p = 0.021), but was reduced to a 1.6+/-0.62 log decrease (n = 5) in viral titers compared to 7.2+/-0.27 log drop (n = 5) in viral titers following OSCNproduction ( Fig 6A). As in the case of IAV strains, both, OSCNand OImediated inactivation of this IBV strain was completely inhibited by catalase, as well (Fig 6A). Since the used dose of Isustained only minor inactivation of the tested IBV strain, we increased the I - concentration to 40 mM to explore the potential improvement of the antiviral action of LPO. Unexpectedly, we did not observe improved inactivation of the B/Yamagata/16/1988 strain by up to 100-fold higher Idoses ( Fig 6B). Thus, the tested IBV strain showed a preference for SCNas the LPO substrate for inactivation.

The substrate preference of influenza B strains for LPO-mediated inactivation is strain-dependent
To test whether the observed, marked difference in susceptibility to OSCNversus OIof the B/Yamagata/16/1988 strain (Fig 6A and 6B) is a unique feature of this strain or is generally true for all IBV strains, we tested two additional IBV strains. The B/Great Lakes/1739/1954 strain that also belongs to the Yamagata lineage [34] was efficiently inactivated (n = 5) by both, OSCN -(6.00+/-0.98 log decrease) and OI -(4.34+/-1.13 log decrease), with a slight preference to OSCN - (Fig 6A). The B/New York/1056/2003 IBV strain belongs to the Victoria lineage and has been isolated from a pharyngeal swab from a human with unspecified respiratory disease (BEI Resources). This Victoria lineage strain was equally inactivated by OSCN -(3.23+/-1.00 log decrease) and OI -(3.23+/-0.75 log decrease, n = 3) (Fig 6A). LPO-mediated inactivation of all three IBV strains was entirely blocked by catalase (Fig 6A). Although there is a trend towards OSCN -, data shown in Fig 6C did not find an overall significant difference between OSCNfueled and OImediated inactivation of IBV strains.

Influenza B viruses have a significantly higher OSCN -/OIsusceptibility ratio than influenza A strains
To explore whether there are differences in the previous readouts between IAV and IBV strains, we compared susceptibilities to OSCNand OI -, as well as, their ratio called "OSCN -/ OIsusceptibility ratio" between the IAV and IBV strains analyzed. As Fig 7 shows, there was no significant difference between the two viral species in their susceptibilities to the virucidal agents. However, we observed a significant difference (p = 0.0182) in their susceptibility ratios as shown in Fig 7 (right panel). All IAV strains had very low ratios (0.73+/-0.13, mean+/-S.E. M., n = 9) while IBV strains had much higher values (4.00+/-2.35, mean+/-S.E.M., n = 3) (Fig  7). Thus, IBV strains tend to be more sensitive to OSCNwhereas IAV strains are equally sensitive to both LPO substrates or more susceptible to OI -.

Substrate specificity map of influenza inactivation by LPO
To summarize our results after surveying 12 different influenza strains for their susceptibilities and LPO substrate preferences in the H 2 O 2 /LPO/(SCN -/I -) system, we plotted our data as a "substrate preference or susceptibility map" shown in Fig 8. The X-axis represents the extent of viral inactivation that is associated with OSCN -, while the Y-axis represents the extent of viral inactivation by OI -. The higher the number, the more susceptible a particular virus strain is to the indicated, LPO-generated antiviral agent (Fig 8). The indicated diagonal crossing the map from the lower left corner to the upper right corner indicates no substrate preference (Fig  8). The H1N2 influenza strain tested localizes to the upper right corner indicating high susceptibility to the LPO-based antimicrobial system with no considerable preference for the LPO substrate (Fig 8). All H1N1 strains appear on the diagonal showing no preference for the LPO substrate. While the A/Brisbane/59/2007 strain can be found in the lower left corner, the A/ California/04/2009, A/Mississippi/3/2001, and A/Turkey/Kansas/4880/1980 strains are, however, all located in the upper right corner, close to the H1N2 strain, representing widely distinct susceptibilities of the tested H1N1 viruses towards the H 2 O 2 /LPO/(SCN -/I -) system. All four H3N2 influenza A strains showed a clear and noteworthy preference for Ias the LPO substrate for their inactivation in the cell-free system and were grouped together into an "H3N2 cluster" (Fig 8). The first IBV strain tested (B/Yamagata/16/1988) mapped into the lower right corner indicating its highly susceptible nature to OSCNbut almost resistance towards OI - (Fig 8). Two further IBV strains, however, failed to confirm this trend and localized to the vicinity of the center of the graph. Overall, our work represents an unexpected complexity of influenza virus inactivation by the H 2 O 2 /LPO/(SCN -/I -) system.

Discussion
Seasonal and pandemic infections by influenza viruses represent a major threat. Seasonal influenza viruses infect 5-15% of the human population annually, resulting in more than half a Influenza inactivation by hypothiocyanite or hypoiodite million deaths worldwide [35]. Strain complexity, virus drift, and viral reassortment make it difficult to develop vaccines providing protection against a wide range of influenza strains. Current trivalent vaccines include one H1N1, one H3N2 IAV strain and one IBV strain of either the Yamagata or Victoria lineages [35]. Quadrivalent vaccines include two influenza B strains, one Yamagata and one Victoria strain, in addition to the two IAV strains [35]. The efficacy of the annual influenza vaccine varies widely from 10-60% [36], and the vaccine does not provide universal influenza virus protection [36]. Currently, all antivirals face the problem of increasing drug resistance [37]. Therefore, there is an urgent need to find novel mechanisms by which influenza can be fought, preferably in a strain-independent fashion.
The H 2 O 2 /LPO/(SCN -/I -) system represents an oxidative, fast-reacting mechanism of the respiratory innate immune system that is capable of killing or inactivating a wide range of pathogens. Our results reported here are one of the first ones to document its efficacy against influenza virus. Our prior results established that the H 2 O 2 /LPO/SCNsystem assembled in in vitro cultures of differentiated, human and rat, tracheobronchial epithelial cells inactivates the A/Swine/Illinois/02860/2009 influenza A strain [9]. Here we confirmed that H 2 O 2 /LPO/SCNsystem behaves in a very similar fashion and inactivates the same H1N2 strain under cell-free experimental conditions. This cell-free system provides us with an important tool to address the specificity and the mechanism of action of the LPO-based system against influenza viruses. It is important to emphasize that influenza viruses are inactivated by the H 2 O 2 /LPO/(SCN -/I -) system in both the cell-free and airway epithelial model [9] in less than 60 minutes. Very few, if any, mechanism of the innate immune system reacts so fast and efficiently.
In the current work we expanded our observation made on the IAV H1N2 strain to other influenza strains. Our central focus has been to address the question of whether SCNor Iserves as a better substrate for the anti-influenza action of LPO. We revealed a LPO substrate preference of influenza inactivation that appears more complex than expected. Based on the current survey of influenza strains we conclude that H3N2 IAV strains are inactivated by OImore effectively than by OSCN -. This is presented as an "H3N2 cluster" on LPO's substrate preference map (Fig 8). We did not find this bias towards Iin case of the other IAVs (H1N1 and H1N2) that were inactivated by the LPO substrates. The potential explanation for this is unclear at the moment but redox-sensitive disulfide bonds may be different among HA or NA subtypes that could help explain this observation [38,39]. Differences in OIor OSCNmediated oxidative modifications of these bonds could lead to altered virion disassembly or inhibition of viral entry into host cells [39,40]. Oxidative stress has been shown to inactivate influenza viruses [41]. Oxidative damage of the lipid components of the viral envelope or the nucleoprotein could be also the results of the antimicrobial action of LPO [41]. Differences in susceptibilities of influenza viruses to polyphenols were linked to viral envelope glycoproteins [42]. OIhas a higher oxidative strength than OSCN - [43]. While the main potential targets of OSCN -/HOSCN are certain SH groups, OI -/HOI can target SH groups, NAD(P)H, reduced pyridine nucleotides, thioether groups and NH2 groups [43]. These molecular differences could form the basis for the observed inactivation patterns of influenza viruses in our study. The virucidal effect of OSCNand OImay be due to their ability to either interact with viral HA or NA directly and interfere with viral cell entry or to cross the outer layer of the viral envelop before oxidizing critical viral elements inside the virion. We know that beneath the lipid membrane of the influenza virus, a viral protein called M1 matrix protein is expressed and may be reacting with OSCNor OIand interfere with viral infectivity [44]. Other viral proteins that are cysteine-rich may be the targets of these antiviral agents. In bacteria, for example, the reported bacterial targets of OSCNare critical cysteine residues found in glycolytic enzymes, such as glyceraldehyde triphosphate dehydrogenase, hexokinase, glycose-6-phosphate dehydrogenase, and aldolase leading to the hypothesis that OSCNeffect on bacterial growth is glycolysis-mediated [45]. Glucose transport and respiration may also be the target of OSCNand OI -. It has been reported to inhibit the activity of urease, which is critical to the ability of H. pylori to alkalinize gastric juice and colonize the stomach [46].
IBVs can cause symptoms similar to IAVs in humans, and are responsible for a large proportion of seasonal influenza infections [30,32]. Far less is known regarding the viral and host determinants of IBV disease pathogenicity [30,32]. The immune response against IBV is affected by yearly trivalent or quadrivalent vaccines and typically is weaker in humans than that against IAVs [30]. NA inhibitors that work well against most strains of IAV are typically less effective for IBVs [30]. Therefore, novel approaches are needed to confront IBV. Our results show that the B/Yamagata/16/1988 strain is sensitive against the H 2 O 2 /LPO/SCNsystem but remains more resistant against the H 2 O 2 /LPO/Isystem. After testing two other IBV strains, this pattern failed to be confirmed suggesting that it could be strain-specific. Testing a larger number of IBV strains is needed for a firmer conclusion. Nevertheless, our findings are the first to show that the H 2 O 2 /LPO/SCNantimicrobial system efficiently inactivates IBV strains, as well.
Given the wealth of historical data showing that these LPO-generated products have antimicrobial effects, they could be used for either therapeutic or prophylactic supplementation before infection or drugs, in combination with current anti-influenza therapies to enhance viral elimination and diminish inflammation. SCNis present in the diet and no serious adverse effects of SCNin humans have been reported at physiological concentrations (reviewed in [47]). Iodide intake has already been proposed to improve viral clearance but has several hurdles. While lower dose of iodide intake is safe and associated with normal thyroid function, recent works have proven that alterations in thyroid function are associated with high iodide supplementation in maternal rats and their offspring [48]. Other findings show that treatment in vivo with an excess of iodide can induce the blockade of thyroid hormone biosynthesis [49] and excess of iodide could induce mononuclear infiltration in salivary gland as recently indicated in a Swiss albino mouse model [50] These results question whether iodide supplementation would work to boost anti-influenza defenses.
In summary, the findings presented here shows that the H 2 O 2 /LPO/(SCN -/I -) system robustly inactivates a wide range of influenza viruses that are the major circulating serotypes and species causing epidemics and are also components of the annual influenza vaccine. These results are very encouraging considering the likely contribution of this mechanism to in vivo influenza clearance and its potential as a target for pharmaceutical intervention.
Supporting information S1 Data. Plaque forming unit results. The original data of the plaque forming unit assays using MDCK cells of the virus strains tested in this work are shown for each experiment organized according to the figures. (XLSX)