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Miora Ralambondrainy, Essia Belarbi, Wildriss Viranaicken, Renata Baranauskienė, Petras Rimantas Venskutonis, Philippe Desprès, Pierre Roques, Chaker El Kalamouni, Jimmy Sélambarom
In vitro comparison of three common essential oils mosquito repellents as inhibitors of the Ross River virus
www.plosone.org
www.plosone.org
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 589.0393 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F15 1 Tf
-6.4961 -1.4976 Td
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2.4614 0 Td
(material)Tj
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Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 554.0598 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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2.4321 0 Td
(leaves)Tj
2.5625 0 Td
(of)Tj
/F14 1 Tf
1.0148 0 Td
[()35(\r)-13()17()4()11()4()2()2()11(\n)]TJ
5.3404 0 Td
[()1()14()7()20()22()7()-4()]TJ
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3.1578 0 Td
(\(DC\))Tj
2.3017 0 Td
(Strapf)Tj
2.6022 0 Td
(and)Tj
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1.7291 0 Td
[()23( )15()4()16()6()2()11(\n)18()12()12()]TJ
5.2271 0 Td
[()-8()20()22()3( )7()10()4( )2(\n)]TJ
/F9 1 Tf
4.2519 0 Td
(L'He)Tj
0 Tc
1.5477 0.051 Td
()Tj
-0.004 Tc
0.3969 -0.051 Td
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2.1203 0 Td
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1.0318 0 Td
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(2014)Tj
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(2015)Tj
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3.6623 0 Td
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2.9027 0 Td
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(of)Tj
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1.0148 0 Td
[()92( )8()7()15()3( )2()-9()4()]TJ
3.7814 0 Td
[()-3()16(\n)18()4()11()4()4( )8()]TJ
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4.5468 0 Td
(\(L.\))Tj
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(December)Tj
4.3483 0 Td
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(plant)Tj
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(samples)Tj
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(the)Tj
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(CAHEB)Tj
-32.292 -1.2983 Td
(\(Coope)Tj
0 Tc
2.5568 0.051 Td
()Tj
-0.004 Tc
0.4025 -0.051 Td
(rative)Tj
2.4661 0 Td
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3.6057 0 Td
(des)Tj
1.5363 0 Td
(Huiles)Tj
2.812 0 Td
(Essentielles)Tj
4.7508 0 Td
(de)Tj
1.1679 0 Td
(Bourbon\),)Tj
4.337 0 Td
(Le)Tj
1.1792 0 Td
(Tampon,)Tj
3.8834 0 Td
(Reunion)Tj
3.6624 0 Td
(Island.)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 487.6724 cm
BT
/F15 1 Tf
1 TL
-0.0033 Tc
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3.926 0 Td
(oil)Tj
1.3039 0 Td
(isolation)Tj
3.8504 0 Td
(and)Tj
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Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 470.7212 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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3.7303 0 Td
(oils)Tj
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(extracted)Tj
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(2.5)Tj
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(\(PG)Tj
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(CC\))Tj
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(\(ZV\))Tj
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(during)Tj
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(3)Tj
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(h)Tj
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(a)Tj
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(apparatus.)Tj
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1.5987 0 Td
(were)Tj
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(decanted)Tj
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2.2053 0 Td
(aqueous)Tj
3.5093 0 Td
(phase,)Tj
2.7042 0 Td
(dried)Tj
2.3414 0 Td
(over)Tj
1.9729 0 Td
(anhydrous)Tj
4.4844 0 Td
(sodium)Tj
3.2258 0 Td
(sulfate)Tj
2.8006 0 Td
(and)Tj
1.7291 0 Td
(filtered)Tj
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(Minisart)Tj
3.6624 0 Td
(filters)Tj
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(\(0.2)Tj
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1.7291 0 Td
[()]TJ
/F9 1 Tf
(m\).)Tj
2.1089 0 Td
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3.4923 0 Td
(were)Tj
2.1203 0 Td
(then)Tj
2.0126 0 Td
(stored)Tj
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(at)Tj
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(4C)Tj
1.7461 0 Td
(in)Tj
1.0318 0 Td
(darkness.)Tj
3.9628 0 Td
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1.7802 0 Td
(chemical)Tj
3.7757 0 Td
(composition)Tj
5.2384 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4627 0 Td
(essen-)Tj
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(tial)Tj
1.4626 0 Td
(oils)Tj
1.6044 0 Td
(was)Tj
1.6951 0 Td
(quantified)Tj
4.3087 0 Td
(by)Tj
1.1848 0 Td
(gas)Tj
1.4797 0 Td
(chromatography-flame)Tj
9.4393 0 Td
(ionization)Tj
4.2803 0 Td
(detector)Tj
3.4923 0 Td
(\(GC-FID\))Tj
4.2349 0 Td
(on)Tj
/F14 1 Tf
1.2756 0 Td
[()1()4()16()-9()-4()]TJ
-34.4577 -1.2983 Td
[()]TJ
/F9 1 Tf
1.6157 0 Td
(gas)Tj
1.4797 0 Td
(chromatograph)Tj
6.4232 0 Td
(and)Tj
1.7292 0 Td
(identified)Tj
4.0591 0 Td
(by)Tj
1.1792 0 Td
(gas)Tj
1.4854 0 Td
(chromatography-time-of-flight-ma)Tj
14.0087 0 Td
(ss)Tj
0.9411 0 Td
(spectrome-)Tj
-32.9213 -1.2982 Td
(try)Tj
1.3436 0 Td
(on)Tj
1.2755 0 Td
(a)Tj
0.652 0 Td
(GC)Tj
/F16 1 Tf
[()]TJ
/F9 1 Tf
(GC-TOF)Tj
5.7827 0 Td
(MS)Tj
1.5873 0 Td
(LECO)Tj
2.7383 0 Td
(Pegasus)Tj
3.3562 0 Td
(4D)Tj
1.4286 0 Td
(system)Tj
2.9367 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 365.3291 cm
BT
/F15 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Cell)Tj
1.8803 0 Td
(culture)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 348.3212 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Human)Tj
3.3165 0 Td
(embryonic)Tj
4.541 0 Td
(kidney)Tj
2.9367 0 Td
(cell)Tj
1.5647 0 Td
(line)Tj
1.7008 0 Td
(HEK293T)Tj
4.269 0 Td
(\(ATCC\))Tj
3.5262 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.4683 0 Td
(kidney)Tj
2.9367 0 Td
(epithelial)Tj
3.8721 0 Td
(cell)Tj
1.559 0 Td
(line)Tj
1.7065 0 Td
(Vero)Tj
-35.1267 -1.2982 Td
(\(ATCC\))Tj
3.5206 0 Td
(were)Tj
2.1203 0 Td
(grown)Tj
2.8006 0 Td
(in)Tj
1.0318 0 Td
(Dulbecco's)Tj
4.5921 0 Td
(modified)Tj
3.8494 0 Td
(Eagle's)Tj
2.931 0 Td
(medium)Tj
3.6056 0 Td
(\(DMEM,)Tj
3.8778 0 Td
(Dutscher,)Tj
4.1159 0 Td
(Issy-les-)Tj
-32.4451 -1.2983 Td
(Moulineaux,)Tj
5.2837 0 Td
(France\))Tj
3.2882 0 Td
(or)Tj
1.0998 0 Td
(modified)Tj
3.8494 0 Td
(Eagle's)Tj
2.931 0 Td
(medium)Tj
3.6057 0 Td
(\(MEM,)Tj
3.1407 0 Td
(Dutscher\))Tj
4.2293 0 Td
(supplemented)Tj
5.8563 0 Td
(with)Tj
1.9956 0 Td
(10%)Tj
-35.2797 -1.2982 Td
(fetal)Tj
1.9105 0 Td
(bovine)Tj
2.9253 0 Td
(serum)Tj
2.7213 0 Td
(heat)Tj
1.9048 0 Td
(inactivated)Tj
4.5978 0 Td
(\(FBS,)Tj
2.3698 0 Td
(Dutscher\))Tj
4.2292 0 Td
(and)Tj
1.7291 0 Td
(completed)Tj
4.4051 0 Td
(with)Tj
2.0012 0 Td
(2)Tj
0.6916 0 Td
(mmol.L)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 526.4503 313.7952 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
6.9998 0 0 6.9998 534.8409 309.3732 cm
BT
/F9 1 Tf
1 TL
-0.0057 Tc
0 0 Td
(L)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 538.5826 309.3732 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-gluta-)Tj
-33.8567 -1.3039 Td
(mine)Tj
2.279 0 Td
(\(Dutscher\),)Tj
4.7962 0 Td
(100)Tj
1.6441 0 Td
(U.mL)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 310.3937 300.8125 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 316.5732 296.3338 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(0.1)Tj
1.9162 0 Td
(mg.mL)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 364.422 300.8125 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 372.7559 296.3338 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(penicillin-streptomycin)Tj
9.6207 0 Td
(\(Dutscher\),)Tj
4.7905 0 Td
(1)Tj
0.6973 0 Td
(mmol.L)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 555.4204 300.8125 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 283.3511 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(sodium)Tj
3.2314 0 Td
(pyruvate)Tj
3.6964 0 Td
(\(Dutscher\))Tj
4.5751 0 Td
(and)Tj
1.7291 0 Td
(250)Tj
/F16 1 Tf
1.6441 0 Td
[()]TJ
/F9 1 Tf
(g.mL)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 374.3433 287.7732 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 382.7338 283.3511 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(amphotericin)Tj
5.6239 0 Td
(\(Dutscher\).)Tj
4.7961 0 Td
(Cells)Tj
2.16 0 Td
(were)Tj
2.1203 0 Td
(maintained)Tj
-32.9723 -1.2982 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6577 0 Td
(humidified)Tj
4.6601 0 Td
(atmosphere)Tj
4.9039 0 Td
(of)Tj
1.0148 0 Td
(5%)Tj
1.457 0 Td
(CO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 351.3259 268.6677 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 357.1086 270.3685 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(at)Tj
0.9524 0 Td
(37C)Tj
2.228 0 Td
(in)Tj
1.0318 0 Td
(Petri)Tj
2.143 0 Td
(dishes.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 242.9858 cm
BT
/F15 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Cytotoxicity)Tj
5.3527 0 Td
(assay)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 225.9779 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Cell)Tj
1.7971 0 Td
(viability)Tj
3.4186 0 Td
(was)Tj
1.6951 0 Td
(measured)Tj
4.1102 0 Td
(using)Tj
2.3924 0 Td
(the)Tj
1.4683 0 Td
(tetrazolium)Tj
4.8246 0 Td
(salt)Tj
1.559 0 Td
(MTT)Tj
2.3358 0 Td
(\(3-\(4,5-dimethylthiazol-2-)Tj
10.4711 0 Td
(yl\)-)Tj
-34.0722 -1.2982 Td
(2,5-diphenyltetrazolium)Tj
9.8645 0 Td
(bromide\))Tj
3.9854 0 Td
(assay)Tj
2.2621 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(].)Tj
2.0749 0 Td
(Vero)Tj
2.2167 0 Td
(or)Tj
1.0998 0 Td
(HEK293T)Tj
4.269 0 Td
(cells)Tj
1.9219 0 Td
(were)Tj
2.1203 0 Td
(seeded)Tj
2.9083 0 Td
(in)Tj
1.0375 0 Td
(96-well)Tj
-33.7604 -1.2983 Td
(plates)Tj
2.5001 0 Td
(\(2)Tj
/F16 1 Tf
[()]TJ
/F9 1 Tf
(10)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 248.3716 204.4346 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(5)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 254.0976 200.0125 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(cells/well\))Tj
4.1952 0 Td
(and)Tj
1.7291 0 Td
(allowed)Tj
3.2882 0 Td
(to)Tj
1.0261 0 Td
(adhere)Tj
2.9254 0 Td
(overnight)Tj
4.0818 0 Td
(at)Tj
0.9525 0 Td
(37C.)Tj
2.4547 0 Td
(Cells)Tj
2.16 0 Td
(were)Tj
2.1203 0 Td
(then)Tj
2.0183 0 Td
(treated)Tj
2.999 0 Td
(21)Tj
1.1736 0 Td
(h)Tj
-36.5326 -1.3039 Td
(at)Tj
0.9524 0 Td
(37C)Tj
2.228 0 Td
(with)Tj
1.9956 0 Td
(each)Tj
2.0295 0 Td
(essential)Tj
3.583 0 Td
(oil)Tj
1.2359 0 Td
(within)Tj
2.8176 0 Td
(a)Tj
0.652 0 Td
(wide)Tj
2.1203 0 Td
(range)Tj
2.4661 0 Td
(of)Tj
1.0148 0 Td
(concentrations)Tj
6.1795 0 Td
(\(0.141130)Tj
/F16 1 Tf
4.6318 0 Td
[()]TJ
/F9 1 Tf
(g.mL)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 544.6488 191.4519 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 550.8283 186.9732 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\).)Tj
0.7823 0 Td
(The)Tj
-35.8636 -1.2982 Td
(essential)Tj
3.583 0 Td
(oils)Tj
1.5987 0 Td
(were)Tj
2.126 0 Td
(solubilized)Tj
4.5127 0 Td
(in)Tj
1.0318 0 Td
(0.4%)Tj
2.1543 0 Td
(DMSO)Tj
3.0728 0 Td
(\(Sigma\).)Tj
3.5886 0 Td
(Following)Tj
4.2179 0 Td
(the)Tj
1.4684 0 Td
(treatment,)Tj
4.3596 0 Td
(20)Tj
/F16 1 Tf
1.1735 0 Td
[()]TJ
/F9 1 Tf
(L)Tj
1.2643 0 Td
(of)Tj
1.0147 0 Td
(5)Tj
0.6973 0 Td
(mg.)Tj
-35.8636 -1.2983 Td
(mL)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 213.5622 165.4299 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 221.9527 161.0078 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(MTT)Tj
2.3357 0 Td
(solution)Tj
3.4809 0 Td
(\(Sigma,)Tj
3.2485 0 Td
(Saint-Quentin)Tj
5.9527 0 Td
(Fallavier)Tj
3.6283 0 Td
(France\))Tj
3.2882 0 Td
(were)Tj
2.1203 0 Td
(added)Tj
2.6589 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.474 0 Td
(96-well)Tj
3.1181 0 Td
(plates)Tj
-34.7752 -1.2982 Td
(and)Tj
1.7292 0 Td
(cells)Tj
1.9217 0 Td
(were)Tj
2.1204 0 Td
(stored)Tj
2.7099 0 Td
(at)Tj
0.9524 0 Td
(37C)Tj
2.228 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(darkness.)Tj
3.9685 0 Td
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2.2847 0 Td
(3)Tj
0.6917 0 Td
(h)Tj
0.7483 0 Td
(of)Tj
1.0148 0 Td
(treatment,)Tj
4.3653 0 Td
(supernatant)Tj
4.9776 0 Td
(was)Tj
1.7008 0 Td
(removed)Tj
-33.9191 -1.3039 Td
(and)Tj
1.7292 0 Td
(replaced)Tj
3.5885 0 Td
(by)Tj
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(10)Tj
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q
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1 J
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BT
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1 TL
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[()]TJ
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(CC)Tj
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Q
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1 j
1 J
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BT
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1 TL
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q
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1 J
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(the)Tj
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576 703.8893 l
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354.8293 690.8893 l
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f*
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354.8293 691.3894 l
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f*
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f*
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f*
184.4751 690.8893 m
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183.975 659.8894 l
f*
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354.8293 659.3893 l
354.8293 690.8893 l
f*
354.8293 659.3893 m
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f*
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f*
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f*
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465.3763 659.8894 l
575.9991 659.8894 l
f*
184.4751 659.3893 m
184.4751 608.8893 l
183.975 608.8893 l
183.975 659.3893 l
f*
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f*
355.3294 659.3893 m
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354.8293 608.8893 l
354.8293 659.3893 l
f*
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354.8293 609.3893 l
f*
465.3763 659.3893 m
465.3763 608.8893 l
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464.8762 659.3893 l
f*
464.8762 608.8893 m
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f*
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465.3763 608.8893 l
465.3763 609.3893 l
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f*
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184.4751 577.3893 l
183.975 577.3893 l
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f*
183.975 577.3893 m
36 577.3893 l
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183.975 577.8893 l
f*
355.3294 608.8893 m
355.3294 577.3893 l
354.8293 577.3893 l
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f*
354.8293 577.3893 m
184.4751 577.3893 l
184.4751 577.8893 l
354.8293 577.8893 l
f*
465.3763 608.8893 m
465.3763 577.3893 l
464.8762 577.3893 l
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f*
464.8762 577.3893 m
355.3294 577.3893 l
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464.8762 577.8893 l
f*
575.9991 577.3893 m
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575.9991 577.8893 l
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575.9991 543.6391 m
36 543.6391 l
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f*
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36 530.589 m
186.7465 530.589 l
h
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0 g
7.9999 0 0 7.9999 36 708.7181 cm
BT
/F15 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Table)Tj
2.5512 0 Td
(1.)Tj
1.1763 0 Td
(Main)Tj
2.4024 0 Td
(characteris)Tj
4.5426 0 Td
(tics)Tj
1.6441 0 Td
(of)Tj
1.063 0 Td
(the)Tj
1.5378 0 Td
(essential)Tj
3.7559 0 Td
(oils.)Tj
0 Tc
-18.6733 -1.8567 Td
(Essentia)Tj
3.4441 0 Td
(l)Tj
0.5102 0 Td
(oil)Tj
14.8537 0 Td
(Major)Tj
2.7992 0 Td
(compone)Tj
3.8481 0 Td
(nts)Tj
1.5165 0 Td
(%)Tj
13.1954 0 Td
(CC)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 368.107 692.5606 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(50)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 375.9874 693.8645 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(\()Tj
/F17 1 Tf
[()]TJ
/F15 1 Tf
(g.mL)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 400.0251 697.4362 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(-1\)a)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 467.3763 693.8645 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(CC)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 478.148 692.5606 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(10)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 486.0283 693.8645 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(\()Tj
/F17 1 Tf
[()]TJ
/F15 1 Tf
(g.mL)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 510.1228 697.4362 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 515.1685 693.8645 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(\))Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 518.0031 697.4362 cm
BT
/F15 1 Tf
1 TL
0 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 36 681.3921 cm
BT
/F14 1 Tf
1 TL
0 0 Td
[()35(\r)-13()17()4()11()4()2()2()11(\n)]TJ
5.3716 0 Td
[()13()14()7()20()22()7()-4()]TJ
/F9 1 Tf
3.4371 0 Td
(\(CC\))Tj
10.1268 0 Td
(Geranial)Tj
3.6851 0 Td
(\(45.11)Tj
/F16 1 Tf
2.7142 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(2.46%\))Tj
-7.2001 -1.1905 Td
(Neral)Tj
2.452 0 Td
(\(32.16)Tj
/F16 1 Tf
2.7142 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.69%\))Tj
-5.967 -1.1835 Td
(Myrcene)Tj
3.7772 0 Td
(\(7.85)Tj
/F16 1 Tf
2.2394 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(1.46%\))Tj
14.5419 2.374 Td
(49.5)Tj
/F16 1 Tf
1.885 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(20.5)Tj
11.0694 0 Td
(17.6)Tj
/F16 1 Tf
1.8921 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(8.2)Tj
/F14 1 Tf
-56.7429 -3.9401 Td
[()23( )15()4()16()6()2()11(\n)]TJ
3.4441 0 Td
[()12()12()]TJ
1.8142 0 Td
[()13()20()22()3( )7()10()4( )2(\n)]TJ
3.9827 0 Td
[()]TJ
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0.5669 0 Td
(\(PG\))Tj
9.1276 0 Td
(Citronellol)Tj
4.578 0 Td
(\(23.43)Tj
/F16 1 Tf
[()]TJ
/F9 1 Tf
3.2811 0 Td
(0.14%\))Tj
-7.8591 -1.1835 Td
(Geraniol)Tj
3.756 0 Td
(\(16.85)Tj
/F16 1 Tf
2.7142 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.05%\))Tj
-7.271 -1.1906 Td
(Citronelly)Tj
4.0465 0 Td
(l)Tj
0.4819 0 Td
(formate)Tj
3.3874 0 Td
(\(12.29)Tj
/F16 1 Tf
2.7072 0 Td
[()]TJ
/F9 1 Tf
0.8078 0 Td
(0.05%\))Tj
-11.4308 -1.1834 Td
(Linalool)Tj
3.5434 0 Td
(\(10.79)Tj
/F16 1 Tf
2.7071 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.05%\))Tj
-7.0513 -1.1906 Td
(Isomenthon)Tj
4.8969 0 Td
(e)Tj
0.6591 0 Td
(\(7.06)Tj
/F16 1 Tf
2.2323 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.02%\))Tj
/F18 1 Tf
12.7702 4.7481 Td
(>)Tj
/F9 1 Tf
0.9921 0 Td
(1000)Tj
12.7631 0 Td
(533)Tj
/F16 1 Tf
1.6583 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(199)Tj
/F14 1 Tf
-56.5091 -6.3072 Td
[()92( )8()7()15()3( )2()-9()4()]TJ
3.8126 0 Td
[()14()-3()16(\n)6()11()4()4( )]TJ
3.9118 0 Td
[()]TJ
/F9 1 Tf
0.567 0 Td
(\(VZ\))Tj
10.6441 0 Td
(Khusimo)Tj
3.6922 0 Td
(l)Tj
0.4819 0 Td
(\(23.78)Tj
/F16 1 Tf
2.7142 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.13%\))Tj
-7.6891 -1.1905 Td
(\()Tj
/F14 1 Tf
[( )]TJ
/F9 1 Tf
(\)-Isova)Tj
3.6922 0 Td
(lencenol)Tj
3.6213 0 Td
(\(6.79)Tj
/F16 1 Tf
2.2323 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.12%\))Tj
/F19 1 Tf
-10.3466 -1.1906 Td
()Tj
/F9 1 Tf
(-Vetivo)Tj
3.5788 0 Td
(ne)Tj
1.2118 0 Td
(\(3.84)Tj
/F16 1 Tf
2.2323 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(0.04%\))Tj
13.5356 2.3811 Td
(169.9)Tj
/F16 1 Tf
2.3669 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(72.2)Tj
10.5875 0 Td
(29.4)Tj
/F16 1 Tf
1.8921 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(13.5)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 36 565.1716 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(a)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 40.4787 561.6566 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(Cytotox)Tj
3.2031 0 Td
(ic)Tj
0.9284 0 Td
(concentration)Tj
5.8607 0 Td
(CC)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 131.0173 560.296 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(50)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 138.6141 561.6566 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(that)Tj
1.8 0 Td
(cause)Tj
2.4094 0 Td
(death)Tj
2.452 0 Td
(to)Tj
1.0418 0 Td
(50%)Tj
ET
Q
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1 j
1 J
0 w
5.9998 0 0 5.3325 36 553.2094 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 40.8755 549.6377 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(Cytotoxic)Tj
4.1386 0 Td
(concentr)Tj
3.5575 0 Td
(ation)Tj
2.2961 0 Td
(CC)Tj
ET
Q
q
1 j
1 J
0 w
5.9998 0 0 5.3325 131.4708 548.3338 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(10)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 139.0677 549.6377 cm
BT
/F9 1 Tf
1 TL
0 0 Td
(that)Tj
1.8 0 Td
(causes)Tj
2.7709 0 Td
(death)Tj
2.4519 0 Td
(to)Tj
1.0418 0 Td
(10%.)Tj
2.1685 0 Td
(Results)Tj
3.0898 0 Td
(are)Tj
1.4598 0 Td
(expressed)Tj
4.1245 0 Td
(as)Tj
1.0346 0 Td
(mean)Tj
/F16 1 Tf
2.4591 0 Td
[()]TJ
/F9 1 Tf
0.8008 0 Td
(SD)Tj
1.4457 0 Td
(from)Tj
2.2252 0 Td
(triplicate)Tj
3.7985 0 Td
(determination.)Tj
0.83 0.64 0.02 0 k
/F11 1 Tf
-0.025 Tc
-43.5546 -2.2464 Td
(https://do)Tj
3.5575 0 Td
(i.org/10.1371/j)Tj
5.4426 0 Td
(ournal.pone)Tj
4.4078 0 Td
(.0196757.t001)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 385.7952 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Essential)Tj
4.2094 0 Td
(oils)Tj
1.6796 0 Td
(repellents)Tj
4.4717 0 Td
(modulate)Tj
4.2591 0 Td
(alphavirus)Tj
4.6913 0 Td
(replica)Tj
2.8772 0 Td
(tion)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
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