Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma

Objective To study the characterization of Fe3O4@Au-C225 composite targeted MNPs. Methods Fe3O4@Au-C225 was prepared by the absorption method. The immunosorbent assay was used to evaluate its absorption efficiency at C225 Fc. ZETA SIZER3000 laser particle size analyzer, ultraviolet photometer and its characteristics were analyzed by VSM. the targeting effect of Fe3O4@Au-C225 composite targeted MNPs on U251 cells in vitro were detected by 7.0 Tesla Micro-MR; and subcutaneous transplanted human glioma in nude mice were performed the targeting effect in vivo after tail vein injection of Fe3O4@Au-C225 composite targeted MNPs by MRI. Results The self-prepared Fe3O4@Au composite MNPs can adsorb C225 with high efficiency of adsorption so that Fe3O4@Au-C225 composite targeted MNPs were prepared successfully. Fe3O4@Au-C225 composite targeted MNPs favorably targeted human glioma cell line U251 in vitro; Fe3O4@Au-C225 composite targeted MNPs have good targeting ability to xenografted glioma on nude mice in vivo, and can be traced by MRI. Conclusion The Fe3O4@Au-C225 composite targeted MNPs have the potential to be used as a tracer for glioma in vivo.


Introduction
The primary malignant central nervous system tumor is among 1.49% of all cancer and the rate of disability and mortality is considerably high [1,2]. Most of these tumors originate from the gliocyte, which is commonly abbreviated to glioma. Despite advances in diagnosis and treatment for it, the prognosis is still disappointing. Therefore, there exists an urgent need for better and more effective treatment. Over the past few decades, neurologist and oncologist have dedicated to understanding the mechanisms of glioma formation and developing methods to stabilize, reduce or even eliminate the tumor.
The nanometer science and technology is developing rapidly nowadays. It motivates more and more interdisciplinary integration between various subjects to create new fields of research and growing branches of the subject. The nanoscale device, with merely 1~100nm in its length, can enter and leave human cells freely. It has the advantages of less volume, better biocompatibility, and superior targeting ability to specific tissus/cells compared to traditional tracers. In our previous study, we synthesized Fe 3 O 4 @Au composite magnetic nanoparticles (MNPs) with crystal growth to optimize the preparation process and its biocompatibility [3]. Gold has a strong capacity in absorbing protein molecules, therefore provides a microenvironment which is similar to the biological molecular ontology environment. Thus, using gold as active core-shell for constructing composited MNPs helps to maintain the reactivity of biological component well [4]. Furthermore, it can enhance the stability of MNPs to improve the binding ability between MNPs and targeted molecules, resulting in stable coupling between MNPs and antibody [5]. Meanwhile, gold can mediate NIR thermotherapy as a sensitizer of light for hyperthermia depended electron-dense, dielectric property, high adsorption cross section and high Light-thermal conversion efficiency [6]. Thus, it can be used for fixing EGFR monoclonal antibody (McAb) cetuximab (C225), constructing new nanocomposite which gathers molecular targeted therapy, MRI, MFH and NIR thermotherapy by preparing Fe 3 O 4 @Au composite MNPs. The preparation and application of Fe 3 O 4 @Au composite MNPs have been reported before the present study, but the research of it used as a carrier to fix commercially available EGFR McAb-C225 for anti-glioma has not been conducted before. This article uses the method of MRI to evaluate the targeting ability of the self-prepared Fe 3 O 4 @Au-C225 composite targeted MNPs in vivo and in vitro.

Main apparatus and reagents
The ultrasonic cleaner used was a CQ50 model (Ultrasonic Instrument Factory, Shanghai). The water-bathing constant temperature vibrator used was an SHZ-22 model (Medical Apparatus Factory, Taicang, Jiangsu). The automatic steam generator used was a ZFQ-B model (Xinhua Medical Apparatus Company, Shandong). The constant current electrophoresis apparatus was purchased from Liuyi Instrument Factory (Beijing). The ultraviolet detector was purchased from Tian Neng Company (Shanghai). The microplate reader used was a Multiskan MK3-353 model (USA). The double-door and dual-temperature refrigerator was purchased from Haier (China). The vacuum drying oven used was a 668 model (Dongtai Electrical Equipment Factory, Jiangsu). The vibrating sample magnetometer (VSM) used was a PPMS-9 model (Quantum Design, USA). A 752 prismatic ultraviolet-visible (UV-vis) spectrophotometer was purchased from Shanghai Precision Scientific Instrument Co, Ltd, (Shanghai, China). The 7.0Tesla Micro-MR was purchased from PharmaScan (Bruker). The Inverted microscope was purchased from Nikon (Japan). Cetuximab Solution for Infusion (C225) was purchased from Merck (Germany). Fe3O4@Au composite MNPs were prepared at the Department of

Targeting ability evaluation of Fe 3 O 4 @Au-C225 composite targeted MNPs in vitro
Glioma cell culture. U251 human glioma cell line was seeded in 10% fetal calf serum high glucose DMED medium. At 37˚C, 5% CO2 conditions in the incubator, passage every 2~3 days, to make the cells adhere to the surface.
Observation of U251 cells labeled by Fe 3 O 4 @Au-C225 composite targeted MNPs in vitro. U251 cells in logarithmic growth phase were seeded in 4 holes of a 6-well plate. About 1 x 106 cells were in each well, the cells in 3 of the 4 holes were labeled after 24h for adhere. The concentration of Fe 3 O 4 @Au was 0.01mg/ml, 0.05mg/ml, 0.1mg/ml and another unlabeled one. After incubation for 24h, the best concentration of probe labeled was observed with an inverted microscope. This concentration was used in all follow-up experiments.
MRI of labeled U251 cells in vitro. U251 cells in logarithmic growth phase were seeded in a 6-well plate at a concentration of 1 x 106 cell/well and divided into group a, b and c after 24 h. Then the cells were incubated for 24 with the Fe 3 O 4 @Au-C225 composite targeted MNPs (group a), Fe 3 O 4 @Au (group b) and Fe 3 O 4 @Au-C225composite targeted MNPs + C225 (40μg/ mL) (group c). 0.25% trypsin was used to digest the cells, after collection and centrifugation, it was resuspended in Eppendorf tube within 0.5ml 1% agarose, and another Eppendorf tube was used as control. 7.0 Tesla Micro-MR (Bruker, PharmaScan 7.0), body coil with an inner diameter of 3 cm and T2-weighted spin-echo images was used for MRI, detailed parameters go as follows: Field of view (FOV) was 5cm x 5cm; layer thickness was 1mm; matrix was 256 x 256; TR was 2000 ms, TE was 36 ms.

Targeting ability evaluation in vivo of Fe 3 O 4 @Au-C225 composite targeted MNPs
Transplanted model of human glioma in nude mice. U251 cells in logarithmic growth phase were digested by 0.25% trypsin and centrifugated. Then, the cells dissolved in 0.9% saline with a concentration of 2 x 106 cells were injected subcutaneously into the right lower extremity of nude mice. 0.15ml solution and 1cm from injection point to the needle point was carried out for injection. The tumors started to grow in inoculation site after 2-3 weeks. It was used in experiment when the diameter of tumor up to 0.5 cm after 4 weeks.
MRI of Fe 3 O 4 @Au-C225 composite MNPs targeted glioma. The mice were randomly divided into group a, b and c, with six mice in each group. Fe 3 O 4 @Au-C225 composite targeted MNPs dissolved in 0.9% saline with a concentration of 100 mg/mL was injected into tail vein of the mice at a dosages of 10mg/kg in group a, the same volume of Fe 3 O 4 @Au composite MNPs was used in group b and Fe 3 O 4 @Au-C225 composite targeted MNPs + C225(40μg/ mL) was used in group c. The changes of MR signal intensity of tumorous were observed by 7.0 Tesla MR Images before and 2h, 8h, 24h and 48h after injection. Imaging sequences include SE-T1WI, SE-T2WI and GRE-T2 Ã WI. The extent and scope of changes and the change over time of tumor signal were evaluated by quantitative analysis.

Preparation and identification of Fe 3 O 4 @Au-C225 composite MNPs
Adsorption immune response and the test of adsorption effect. The adsorption effect of Fe 3 O 4 @Au-C225 composite MNPs on C225 was tested by the dilute solution of the HRPlabeled antibody with 500, 1000, 2000, 4000, 8000, 16000 times, the OD value was tested from supernate. The mixed solution of Fe 3 O 4 @Au-BSA and C225 was used as controls. (Table 1).

The size distribution and Zeta potential of Fe 3 O 4 @Au-C225 composite targeted
MNPs. The self-prepared Fe3O4@Au-C225 composite MNPs were approximately spherical and uniform in size as observed by TEM and SEM (Fig 1A and 1B).The average diameter of Fe 3 O 4 @Au-C225 composite targeted MNPs was 46nm, showed unimodal distribution and narrow size distributed (Fig 1C). The Zeta potential value of Fe 3 O 4 @Au-C225 composite targeted MNPs was 11.1±1.8 mV in a neutral environment of pH = 7.4 ( Fig 1D).
Optical properties of Fe 3 O 4 @Au-C225 composite targeted MNPs. The absorption peaks of Fe 3 O 4 @Au solution was 612nm and Fe 3 O 4 @Au-C225 solution was 630nm. Not only the absorption peaks of Fe 3 O 4 @Au-C225 was decrease but also appeared obvious red shift at the same dilution multiple. This indicated that C225 was well adsorbed on the surface of gold nanoparticle. (Fig 1E curve b) Magnetic properties of Fe 3 O 4 @Au-C225 composite targeted MNPs. The magnetic hysteresis loop showed that the magnetism increased along with the increase of the intensity of externally applied magnetic field of Fe 3 O 4 @Au-C225 composite targeted MNPs at 300k, but they tend to be saturated eventually. The magnetization intensity was tended to 0 when the externally applied magnetic field was dropped gradually to 0, and tend to be saturated in reverse by applied a magnetic field reversed. The magnetic hysteresis loop was closed to coincidence S-curve and showed good superparamagnetism and low remanence and coercivity. The saturation magnetization (Ms) of Fe 3 O 4 @Au-C225 composite targeted MNPs was 51.2emu/g, it was closed to the Ms of Fe 3 O 4 @Au-C225 composite MNP which was 51.8emu/g ( Fig 1F).

Targeting ability evaluation of Fe 3 O 4 @Au-C225 composite targeted MNPs in vitro
Morphology observation of tumour cell and concentration exploration of labelled cells. Under reverse microscopy, the U251 cells were homogeneous, transparent and irregularly flat polygonal. They were arranged in single layer tightly, and the size and shape showed diversity. The spindle cell and polygonal cells which have relatively large nucleus were more commonly existed. They have abundant cytoplasm, obvious cellular atypia and different-sized nucleus. And the nucleus showed a large percentage and an increase hyperchromasia and nucleoli. The U251cells were labeled by Fe 3 O 4 @Au-C225 composite targeted MNPs at the concentration of Fe 3 O 4 @Au of 0.01mg/ml and 0.05mg/ml and 0.1mg/ml (Fig 2A). It was showed that, the internalization of Fe 3 O 4 @Au-C225 composite targeted MNPs by U251 cells was less at 0.01mg/ml, and the background was clear and there was no extracellular particle aggregation; the internalization was more, the background was clear and there was no extracellular particle aggregation at 0.05mg/ml; the internalization was more, but there was more extracellular particle aggregation at 0.1mg/ml. So we chose 0.05mg/ml as the label concentration for further study.
MRI in vitro. The MRI in vitro showed that, the signal intensity in T2WI was obviously reduced contrasted with control group after the U251 cells were incubated with Fe 3 O 4 @Au- C225 composite targeted MNPs (group a, targeting group). Then the signal intensity in T2WI was no obviously reduced after the U251 cells were incubated with Fe 3 O 4 @Au composite MNPs (group b, non-targeting group). And the signal intensity in T2WI was also no obviously reduced after the U251 cells were incubated with Fe 3 O 4 @Au-C225 composite targeted MNPs + C225 (group c, targeting inhibition group) (Fig 2B and 2C).

Targeting ability evaluation in vivo of Fe 3 O 4 @Au-C225 composite targeted MNPs
Transplanted model of human glioma in nude mice. The tumor forming rate of U251cells was about 70%.
MRI of Fe 3 O 4 @Au-C225 composite targeted MNPs targeted glioma in nude mice. The MRI in vivo showed that, the signal intensity and the change rate of tumor were significantly  reduced at different time points both in T2WI and T2 Ã WI after injecting Fe3O4@Au-C225 composite targeted MNPs, which has statistics difference. Although the signal showed slight decrease in group b and group c at 2 h and 8 h after injection, the decrease was less pronounced than group a, and took a shorter duration. The signal intensity of group a was still maintained at a lower level at 24h after injection, and continued to decrease with time. By contrast, the signal intensity of group b and group c were recovered to plain scan level at 24h after injection (Fig 3A and 3B).

Preparation and identification of Fe 3 O 4 @Au-C225 composite targeted MNPs Prepared scheme of Fe 3 O 4 @Au-C225 composite targeted MNPs.
Recently, the molecular pathology study of glioma showed that the overexpression of EGFR is always present in patients with malignant glioma [7]. The overexpression of EGFR is related to poor prognosis, a shortened disease-free period and drug resistance [8][9][10]. EGFR is the first member of the erbB tyrosine kinase family, encoded by the proto oncogene, to be found. The major endogenic ligand of EGFR are EGF and transforming growth factor α (TGF-α). They play an important role in the proliferation of normal and malignant epithelial cells. The EGFR-dependent downstream signaling transduction pathway mainly includes RAS/MAPK, PI3K/Akt, STAT and PLCγ [11]. These pathways can promote cell proliferation, inhibit apoptosis and differentiation, increase the expression level of the VEGF, and promote the invasion and  distant metastasis of tumors [12][13][14]. Thus, EGFR is undoubtedly a preferred key gene and target site in terms of treatment of glioma.
C225 is a human-mouse chimeric IgG1 monoclonal antibody. It competes with EGF and TGF-α to bind with the ligand in the extracellular domain of EGFR. It inhibits the autophosphorylation of EGFR, blocking downstream signaling transduction pathways, and inhibits signal transmission of cell mitosis, which is activated by growth factor and prevents tumor cell proliferation [15]. In addition, the combination of C225 and EGFR can also induce the engagement of receptors in dimerization, internalization and down-regulation [16]. C225 can inhibit the progression of the cell cycle from the G1 stage to S stage, induce apoptosis of tumor cells, inhibit tumor angiogenesis and inhibit the invasion and metastasis of tumors by a variety of activators [17]. Increased antitumor effects of cytotoxic drugs and restored sensitivity of  resistant cells were observed when C225 was combined with antitumor drug [18]. C225 can prevent radiation injuries of tumor cells.
C225 is currently the first drug approved for use on head and neck tumors by the FDA [19]. This is due to its excellent ability to target EGFR and its synergistic effect with chemotherapy and radiation. In order to explore whether or not it has a synergistic effect with the thermotherapy of glioma, EGFR-targeted composite MNPs are constructed using MNPs as a carrier and C225 as a target molecule, where C225 is fixed on the MNPs surfaces. In our previous research, the Fe 3 O 4 @Au composite MNPs with a good biocompatibility was prepared [3]. Additionally, using the absorptive abilities of antibodies on gold surfaces, Fe 3 O 4 @Au-C225 composite MNPs was successfully made through ionic and hydrophobic interaction. (Fig 4A) Adsorption of C225 and the test of adsorption effect. Presently, common methods used for antibody fixation include adsorption, entrapment, crosslinking and covalent binding. Adsorption is a method used to fix antibodies on solid surface through physical adsorption [20]. The method generally involves the immersion of a solid state transducer in a solution containing antibodies, after the solid surface has undergone appropriate treatment or modification, or consists of dropping the solution on the solid surface for adsorption via polar bonds, hydrogen bonding, hydrophobic interactions and interactions between electrons. The adsorption methods commonly includes: (1) Nano particle adsorption: the absorption between gold nanoparticle and antibody to fix the antibodies on the surface of gold nanoparticles [21]. (2) Self-assembly absorption: the characteristic interaction between the sulfenyl of antibody itself, and gold to fix onto the surface of gold [22][23].
The method of adsorption to fix C225 on the Au shell of Fe 3 O 4 @ Au composite MNPs was adopted to maintain maximum antibody activity after fixation. Other methods result in reactions that are difficult to control, large losses of antibody activity, or be complicated to operate with high costs. According to the colloidal gold labelled technique, the adsorption of gold nanoparticles onto a protein mainly depends on pH levels. They can easily form a solid combination under the conditions close to the protein isoelectric point or in alkaline conditions. The combination between gold nanoparticles and IgG was the most stable at a pH of 9.0. The combination was currently considered of the adsorption by electrostatic interaction between negative charge on the surface of gold and positive charge group of antibody and form a solid combination by stable chemical bond such as Au-S [24][25][26][27]. The Fe 3 O 4 @Au composite MNPs prepared was an average size of 35 nm with a negative charged on the surface (-23.2±1.8 mV), and was able to immobilize IgG monoclonal antibody C225 effectively. (Fig 4B) The optimum amount of C225 absorbed was based on the salts that affect the adsorption of gold colloids on antibodies to make the sol coagulation. The results showed that, Fe 3 O 4 @Au composite MNPs solution can be stable with 30-60μg/ml C225, which means the proportion of C225 to Fe 3 O 4 @Au composite MNPs was appropriate under this mass concentration range. 40μg/ml of C225 solution was chosen for adsorption to obtain the best adsorption effect and to save cost. BSA is widely used in biochemical experiments. It was added in the enzyme-cutting reaction buffer to protect the enzyme by improve the concentration of protein in the solution. That can prevent the decomposition of enzymes and non-specific adsorption, which can reduce the degeneration caused by adverse environmental factors such as heating, surface tension and chemical factors of some enzymes.
The specific action of antibody and other biomolecules was due to their specific functional areas, such as the Fab-terminal of antibody recognizing the corresponding antigen, but Fc-terminal not having this ability [28]. In order to maintain the bioactivity of antibody at a maximum, the fixed adsorption and other modifications of the antibody can only be carried out at the Fc-terminal. So, the Fc-terminal of the antibody is clearly crucial to the adsorption condition of Fe 3 O 4 @Au composite MNPs. The adsorption condition of Fe 3 O 4 @Au composite MNPs and C225 was studied by Eenzyme-Llinked Iimmunoadsorbent Aassay (ELISA), which is common. The adsorption efficiency of Fe 3 O 4 @Au-C225 composite MNPs on Fc-end of C225 can be obtained when there is complete binding capacity of Goat Anti-Human Fc-HRP to exposed Fc-end of C225. The Fe 3 O 4 @Au composite MNPs can not only be formed with antibodies but also from precipitation by magnetic separation. The Fe 3 O 4 @Au was considered to adsorb either the Fc-end or Fab-end after C225 was added into Fe 3 O 4 @Au solution. The Fc-end of C225 cannot combine with Goat Anti-Human Fc-HRP after it was adsorbed by Fe 3 O 4 @Au. Then the Fc-end of C225 without combined with Fe3O4@Au can be combined with Goat Anti-Human Fc-HRP. After that, it must lead to a decrease of Goat Anti-Human Fc-HRP in supernatant by magnetic separation. If Fc-end of C225 was completely combined with Fe 3 O 4 @Au, the quantity of Goat Anti-Human Fc-HRP would not decrease after centrifugation. Thus, the Fe 3 O 4 @Au composite MNPs-C225-Goat Anti-Human IgG complex can be separated from a solution by magnetic separation after the second antibody of Goat Anti-Human IgG Fc-HRP is added into a solution of Fe 3 O 4 @Au composite MNPs adsorbed C225. Then, the enzyme labeled antibody, which is not combined with C225, is reserved in the solution. It can result in coloration after the addition of a substrate. The OD value can be obtained via adding enzyme substrate for coloration. If same concentrations of C225 solution and Goat Anti-Human IgG are reacted at the same time, the OD value can be measured by coloration, using the addition of substrates. Then the adsorption rate of Fe 3 O 4 @Au composite MNPs on the Fc-terminal of C225 can be obtained from the difference between two OD values. The mixed solution was used as a control to calculate the adsorption efficiency to avoid the affection of coloration by Fe 3 O 4 @Au in experiment. The results showed that the absorption efficiency of Fe 3 O 4 @Au composite MNPs on the Fc-terminal of C225 was above 90%, which meant that the adsorption effect of Fe 3 O 4 @Au composite MNPs on C225 was relatively good. This procedure was simple, and an ideal result was obtained by the enzyme-linked immunosorbent assay after magnetic separation and coloration of enzyme labelled antibody.
Characterization of Fe 3 O 4 @Au-C225 composite targeted MNPs. The Fe 3 O 4 @Au-C225 composite targeted MNPs can be identified by laser particle size analysis, Zeta potential, UV-Vis spectrum, magnetic hysteresis loop and more. The average diameter of Fe 3 O 4 @Au-C225 composite targeted MNPs is 46nm, and shows unimodal and narrow size distribution by laser particle size analysis. Compared to Fe 3 O 4 @Au, which has an average diameter of 35nm, it was slightly increased. This was likely caused by the increase of particle size in the aqueous phase when a protein layer is formed by the adsorption of Fe 3 O 4 @Au surface on C225. The zeta potential value of Fe 3 O 4 @Au-C225 composite targeted MNPs was 11.1±1.8 mV in the neutral environment at a pH of 7.4. This means that C225 was adsorbed on the surface of Fe 3 O 4 @Au composite MNPs. The surface charge was also changed to positive charge. The maximum absorption peak of Fe 3 O 4 @Au composite MNPs at 612nm can be seen in the UV-Vis spectra, whilst the maximum absorption peak of Fe 3 O 4 @Au-C225 composite targeted MNPs was at 630nm. The obvious red-shift showed that antibodies were adsorbed on the surface of gold, a result which was consistent with other studies [29,30]. The magnetic hysteresis loop of Fe 3 O 4 @Au-C225 composite targeted MNPs was closed to the coincidence S-curve that is similar to Fe 3 O 4 @Au composite MNPs, showing good superparamagnetism, and low remanence and coercivity. The Ms of Fe 3 O 4 @Au-C225 composite targeted MNPs was 51.2emu/g, and was reserved in the Ms of Fe 3 O 4 @Au-C225 composite MNP (51.8emu/g).

Targeting ability evaluation of Fe 3 O 4 @Au-C225 composite targeted MNPs by MRI
The ideal tracer for displaying microinvasive lesions of tumors should have good resolution, sensitivity, specificity and safety. While the sensitivity of MRI is lower than nuclear medicine imaging, the MRI has a longer effective imaging time window, higher temporal and spatial resolution and better degree of contrast. Thus, MRI shows a wider application foreground in tracing tumor cells in vivo [31,32]. The Fe 3 O 4 @Au-C225 composite targeted MNPs synthesized in our previous study can target EGFR in glioma specifically, and the magnetic property shows super paramagnetic, and a high degree of biocompatibility [3]. So the imaging of glioma cells was taken to approach the effect of MRI on Fe 3 O 4 @Au-C225 composite targeted MNPs in vivo and in vitro by 7.0 Tesla Micro-MR, and showed the potential of tracing glioma cells in vivo by Fe 3 O 4 @Au-C225 composite targeted MNPs.

Targeting ability of Fe 3 O 4 @Au-C225 composite targeted MNPs in vitro.
The superparamagnetism of Fe 3 O 4 @Au-C225 composite targeted MNPs can produce an evident negative contrast effect to reduce target area signal in MR image. The target cell was observed and traced in MR image. The changes in the signals of U251 cells incubated with Fe 3 O 4 @Au-C225 composite targeted MNPs in each sequence of T2 Ã WI, T2WI and T1WI was compared with the agarose control group in the experiment of imaging in vitro by MR. The changes in the signal of T2WI and T2 Ã WI were the most obvious, which was consistent with the results of published SPION MRI [33,34] @Au-C225 composite targeted MNPs, which is able to easily approach the negatively charged membrane and enter cells. C225 mediates the cell endocytosis of composite targeted MNPs. It was also difficult for Fe 3 O 4 @Au composite MNPs to approach and enter U251 cells, which has a negative charge on the surface. The signal intensity of T2WI and T2 Ã WI decreased scarcely after Fe 3 O 4 @Au-C225 composite targeted MNPs were incubated with C225 by adding nutrient medium into U251 cells. This means that Fe 3 O 4 @Au-C225 composite targeted MNPs entering U251 cell was significantly reduced. It is suggested that the receptor-mediated endocytosis of Fe 3 O 4 @Au-C225 composite targeted MNPs is interrupted by dissociative C225, which shows that the entrance of Fe 3 O 4 @Au-C225 composite targeted MNPs into U251 cell was mainly dependent on receptor-mediated endocytosis ( Fig 4C).

Targeting ability of Fe 3 O 4 @Au-C225 composite targeted MNPs in vivo.
The transplanted model of human glioma in nude mice made was evaluated on targeting by MRI in vivo, in reference to the MR imaging characteristics of Fe 3 O 4 @Au-C225 composite targeted MNPs in vitro. The results of MRI in vivo show that the intensity and signal change rate of tumor tissues in T2WI and T2 Ã WI at various time points were all decreased after tail vein injection of Fe 3 O 4 @Au-C225 composite targeted MNPs. The most evident difference in statistics can be noted in T2 Ã WI. This may be due to the difference of T2 Ã WI in regards to magnetic susceptibility, where it was more sensitive. The magnetic sensitive effect caused by ferrum was also greater. The signals of stem cell in the kidney and liver, which are labelled with SPIO via renal vein and portal vein, were traced in MRI made by Bos et al. The results show that the decreased effect of imaging signals of T2 Ã WI were the most noticeable, and showed a linear correlation with the dose of iron particles [33]. Kustermann found that the size of the attenuating region of myocardial infarction signals remained consistent in the proton density weighted image and T2 Ã WI image both before and after the injection of labeled cells. However, after the injection of cell labeled iron particles, the size of the proton density weighted image was larger than the T2 Ã WI image. That means T2 Ã WI is more sensitive to SPION, and the target area is increased by the reduced effect of magnetic susceptibility signal [35]. Although the signals of Fe 3 O 4 @Au and Fe 3 O 4 @Au-C225 composite targeted MNPs with C225 were both decreased slightly after the injection at the 2 hour and 8 hour mark, but the decrease was smaller than those of Fe 3 O 4 @Au-C225 composite targeted MNPs and the duration was short. The signal returned to a plain scan level at 24h after the injection of Fe 3 O 4 @Au composite MNPs. By contrast, the signal intensity was still maintained at a lower level at 24h after injection of Fe 3 O 4 @Au-C225 composite targeted MNPs, and showed a decreasing trend with progression of time. This suggest that the content of Fe 3 O 4 @Au-C225 composite targeted MNPs is greater in glioma tissues and is mainly gathered in glioma U251 cells, without the phagocytic degradation by mononuclear phagocyte system via receptor-mediated endocytosis of C225. This is consistent with the results shown in the MRI of U251 cell in vitro. Due to the high targeting of tumor cells and obvious imaging effect on T2WI and T2 Ã WI sequences, the Fe 3 O 4 @Au-C225 composite targeted MNPs can be used as MRI negative contrast agents to trace glioma in vivo.

Conclusions
In this study, we discovered that the Fe 3 O 4 @Au-C225 composite targeted MNPs have good superparamagnetism and optical properties. And it could be excellent negative contrast media for MRI. The favorable targeting to glioma in vitro and in vivo and biocompatibility showed that they have the potential to be used as a tracer for glioma in vivo.